Experimental Animals最新文献_第4页

Loss of TMC7 causes male infertility with disrupted spermiogenesis in mice. 在小鼠中，TMC7缺失会导致雄性不育并破坏精子发生。

IF 2 4区农林科学

Experimental Animals Pub Date : 2026-07-30 DOI: 10.1538/expanim.26-0078

Yumiao Qiu, Yuki Hiradate, Chen Pan, Anh Hoang Pham, Chihiro Emori, Maki Kamoshita, Mengjiao Luo, Masahito Ikawa, Keisuke Shimada

{"title":"Loss of TMC7 causes male infertility with disrupted spermiogenesis in mice.","authors":"Yumiao Qiu, Yuki Hiradate, Chen Pan, Anh Hoang Pham, Chihiro Emori, Maki Kamoshita, Mengjiao Luo, Masahito Ikawa, Keisuke Shimada","doi":"10.1538/expanim.26-0078","DOIUrl":"https://doi.org/10.1538/expanim.26-0078","url":null,"abstract":"Spermatogenesis is a highly ordered developmental process that occurs in the seminiferous tubules and involves mitotic proliferation of spermatogonial stem cells, meiotic division of spermatocytes, and post-meiotic spermiogenesis, ultimately producing mature spermatozoa. Defects at any stage of this process can lead to male infertility. Large-scale transcriptomic and proteomic studies have identified thousands of testis-enriched genes in humans and mice, supporting the use of mouse models to uncover genetic regulators of male fertility. In this study, we focused on transmembrane channel-like protein 7 (TMC7), which is highly expressed in the testis. To investigate its physiological function, we generated a Tmc7 knockout (KO) mouse line using the CRISPR/Cas9 system. Tmc7 KO male mice were sterile, and no spermatozoa were observed in the epididymis. Instead, multinucleated giant cells containing multiple elongating spermatids were detected in the lumens of seminiferous tubules. We found that these abnormalities were associated with defective intercellular bridge (ICB) stabilization. Transmission electron microscopy further revealed that spermatid nuclei passed through gaps within the ICBs, leading to the formation of multinucleated giant cells. Given the Golgi localization of TMC7, ICB destabilization may occur secondarily due to blood-testis barrier disruption caused by alterations in the testicular microenvironment. Importantly, ectopic expression of TMC7 in Tmc7 KO mice rescued the defective spermiogenesis phenotype. Taken together, these findings demonstrate that TMC7 plays a critical role in spermiogenesis and indirectly contributes to the maintenance of ICB integrity.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148619005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The peroneal muscular atrophy mousehas aberrant projection of spinal motor axons to the gluteal region. 腓骨肌萎缩小鼠脊髓运动轴突向臀区异常投射。

IF 2 4区农林科学

Experimental Animals Pub Date : 2026-07-30 DOI: 10.1538/expanim.26-0082

Kazuko Keino-Masu, Yuta Mitani, Takuya Okada, Takuya Amanuma, Hideki Katoh, Masayuki Masu

{"title":"The peroneal muscular atrophy mousehas aberrant projection of spinal motor axons to the gluteal region.","authors":"Kazuko Keino-Masu, Yuta Mitani, Takuya Okada, Takuya Amanuma, Hideki Katoh, Masayuki Masu","doi":"10.1538/expanim.26-0082","DOIUrl":"https://doi.org/10.1538/expanim.26-0082","url":null,"abstract":"The peroneal muscular atrophy (pma) mouse shows congenital clubfoot due to atrophy of the peroneal muscles caused by lack of peroneal nerve innervation. Although it was reported that the peroneal nerve was absent in pma embryos, specific analysis of motor axon development in pma embryos has not been performed. Here, we generated a transgenic pma mouse that expresses enhanced green fluorescent protein (EGFP) in the spinal motor neurons, and examined the motor nerve trajectory specifically using wholemount preparations and three-dimensional reconstruction of EGFP-immunostained fibers. At embryonic day 11.5, when the sciatic nerve began to grow out of the spinal cord, no apparent differences were observed between the control and pma mice. From embryonic days 12.0 to 13.5, in the control mouse, the peroneal and tibial nerves extended into the hindlimb bud, whereas in the pma mouse, the tibial nerve formed normally but the peroneal nerve was never observed in its proper position. Moreover, an abnormal EGFP-positive axon bundle was observed near the gluteal nerve in the hip region. These results indicate that the pma mouse has axon guidance defects in the spinal motor neurons in addition to lack of the peroneal nerve.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148619050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Changes in microbial prevalence in mice within Japanese academic animal facilities between 2011 and 2024. 2011年至2024年间日本学术动物设施中小鼠微生物流行率的变化。

IF 2 4区农林科学

Experimental Animals Pub Date : 2026-07-30 DOI: 10.1538/expanim.26-0041

Hanako Morita, Mai Tanaka, Yurina Tomiyama, Masafumi Yamamoto, Tomoko Ishida, Masahiko Yasuda, Masami Ueno, Nobuhito Hayashimoto

{"title":"Changes in microbial prevalence in mice within Japanese academic animal facilities between 2011 and 2024.","authors":"Hanako Morita, Mai Tanaka, Yurina Tomiyama, Masafumi Yamamoto, Tomoko Ishida, Masahiko Yasuda, Masami Ueno, Nobuhito Hayashimoto","doi":"10.1538/expanim.26-0041","DOIUrl":"https://doi.org/10.1538/expanim.26-0041","url":null,"abstract":"The microbiological quality of laboratory animals evolves along with changes in the research environment. To identify recent trends in modern Japanese research facilities, this study analyzed longitudinal contamination data for 16 major pathogens (including Helicobacter hepaticus, Pseudomonas aeruginosa, Staphylococcus aureus, Mycoplasma pulmonis, Mouse hepatitis virus (MHV), Murine norovirus (MNV) and various protozoa) collected over a 14-year period from 2011 to 2024. Our analysis revealed a significant decline in the testing request-level positivity rates (the number of positive testing requests per total requests) for most pathogens, including Helicobacter hepaticus, Pasteurella pneumotropica, Staphylococcus aureus, MHV, MNV and several intestinal protozoa and pinworms. Notably, the highest positivity rate, recorded for Staphylococcus aureus, decreased from 21.45% in 2011 to 13.65% in 2024. Similarly, the rate for Entamoeba dropped from 9.75% to 5.76%. Spironucleus muris, which maintained a low prevalence, showed a slight increase from 0.05% to 0.09% during the same period. These results demonstrate that the overall microbiological quality of mice from academic institutions has improved significantly over the past 14 years. While this progress reflects the success of modern bio-exclusion strategies, the data indicate that certain microorganisms persist at low levels and are not easily eradicated. These findings suggest that maintaining this high standard of quality requires continuous vigilance and the further refinement of quality control protocols to safeguard the integrity and reproducibility of biomedical research.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148618895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-invasive individual identification of adult Pleurodeles waltl using Deep Learning. 利用深度学习的非侵入性成年侧耳虫个体识别。

IF 2 4区农林科学

Experimental Animals Pub Date : 2026-07-30 DOI: 10.1538/expanim.26-0089

Kosuke Kagamiyama, Naofumi Saiki, Kosuke Takaya, Atsuro Koga, Masaru Ueki, Toshinori Hayashi, Tetsuya Ohbayashi

{"title":"Non-invasive individual identification of adult Pleurodeles waltl using Deep Learning.","authors":"Kosuke Kagamiyama, Naofumi Saiki, Kosuke Takaya, Atsuro Koga, Masaru Ueki, Toshinori Hayashi, Tetsuya Ohbayashi","doi":"10.1538/expanim.26-0089","DOIUrl":"https://doi.org/10.1538/expanim.26-0089","url":null,"abstract":"Reliable individual identification is essential for long-term tracking and reproducible laboratory animal studies. In amphibians, invasive marking methods like tags and dye injections can cause welfare concerns and may be unreliable because of tag loss or migration. We developed and evaluated a non-invasive identification system for 25 adult Pleurodeles waltl using smartphone-captured images and the pre-trained convolutional neural network EfficientNetV2. To determine the most informative imaging region, separate models were trained and tested using head and whole-body images. The head-image model achieved 95.3% accuracy on the independent test dataset (macro F1-score = 0.946; Cohen's kappa = 0.951), markedly outperforming the whole-body model (56.6% accuracy). Grad-CAM visualization showed that the model primarily focused on dorsal head spot patterns, indicating that these markings are more informative for individual recognition than whole-body patterns. Because this method requires only a smartphone and a trained model, it can be implemented without specialized marking equipment. This approach enables accurate individual identification, while avoiding invasive marking and therefore supports both animal welfare and research reproducibility. It may provide a practical basis for standardizing individual identification in future amphibian research.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148618990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Involvement of CD72 in membranoproliferative glomerulonephritis induced by Plasmodium chabaudi AS infection in NC/Jic mice. CD72参与NC/Jic小鼠chabaudi疟原虫感染所致的膜增殖性肾小球肾炎。

IF 2 4区农林科学

Experimental Animals Pub Date : 2026-07-30 DOI: 10.1538/expanim.26-0062

Yuki Miyasaka, Shunsuke Fujii, Yuko Shibata, Yuta Masuda, Yoshiaki Kikkawa, Masashi Mizuno, Tamio Ohno

{"title":"Involvement of CD72 in membranoproliferative glomerulonephritis induced by Plasmodium chabaudi AS infection in NC/Jic mice.","authors":"Yuki Miyasaka, Shunsuke Fujii, Yuko Shibata, Yuta Masuda, Yoshiaki Kikkawa, Masashi Mizuno, Tamio Ohno","doi":"10.1538/expanim.26-0062","DOIUrl":"https://doi.org/10.1538/expanim.26-0062","url":null,"abstract":"NC/Jic mice infected with the rodent malaria parasite Plasmodium chabaudi AS (P. chabaudi AS) develop membranoproliferative glomerulonephritis (MPGN), a Type III hypersensitivity reaction. MRL/MpJ-lpr/lpr (MRL/lpr) mice, a murine model of systemic lupus erythematosus, also develop nephropathy, which is classified as a Type III hypersensitivity reaction. This nephropathy in MRL/lpr mice involves cluster of differentiation 72 (CD72), a negative regulator of B cell activation. NC/Jic mice harbored the same Cd72c haplotype as MRL/lpr mice. This haplotype is characterized by a seven amino acid deletion and 13 amino acid substitutions in the C-type lectin-like domain (CTLD) encoded by exon 8. In the present study, we investigated the contribution of the Cd72c haplotype to the development of MPGN in NC/Jic mice. NC/Jic-Cd72 knock-in (NC-Cd72 KI) mice, in which exon 8 of NC/Jic mice was replaced with normal exon 8 derived from the Cd72b haplotype, developed MPGN to a degree comparable to that in NC/Jic mice. In contrast, NC/Jic mice lacking exon 8 of Cd72 (NC-Cd72 Δex8) exhibited significantly attenuated MPGN severity. These results suggest that exon 8 of Cd72 is involved in the development of malaria-induced MPGN in NC/Jic mice, whereas the Cd72c haplotype is not the primary cause.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148618869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

T cell transfer into immunodeficient mice uncovers a paralytic phenotype beyond colitis. T细胞转移到免疫缺陷小鼠中，发现结肠炎以外的麻痹表型。

IF 2 4区农林科学

Experimental Animals Pub Date : 2026-07-09 DOI: 10.1538/expanim.26-0035

Rumi Katsumoto, Shin Ebihara

{"title":"T cell transfer into immunodeficient mice uncovers a paralytic phenotype beyond colitis.","authors":"Rumi Katsumoto, Shin Ebihara","doi":"10.1538/expanim.26-0035","DOIUrl":"https://doi.org/10.1538/expanim.26-0035","url":null,"abstract":"Adoptive transfer of CD45RBhigh T cells into immunodeficient mice is a well-established model for inducing colitis. Here, we report for the first time that a subset of these mice unexpectedly developed paralysis, a phenotype resembling experimental autoimmune encephalomyelitis (EAE). Paralytic mice exhibit the hallmark features of central nervous system (CNS) pathology, including spinal cord demyelination and immune cell infiltration, with prominent involvement of ex-Th17 (also called Th1-like) cells. The presence of ex-Th17 cells in the CNS of the affected mice suggests their contributory role in the development of paralysis. Notably, ex-Th17 cells have also been implicated in the pathogenesis of T cell transfer-induced colitis. Given the shared involvement of ex-Th17 cells in both the colitis model and the EAE-like CNS autoimmune model, we conducted comparative single-cell RNA sequencing analyses of ex-Th17 populations from colonic samples of patients with Crohn's disease and cerebrospinal fluid samples of patients with multiple sclerosis. These analyses revealed a convergent upregulation of the IFN-γ signaling pathway in both disease contexts. Collectively, our findings establish this model as a valuable platform for investigating how identical immune challenges can lead to tissue-specific autoimmune pathology and highlight ex-Th17 cells as a potential mechanistic link between systemic and CNS autoimmunity.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148410976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative study of renal filtration barrier structure and key gene expression between Lepus yarkandensis and Oryctolagus cuniculus. 小黄羊与小黄羊肾滤过屏障结构及关键基因表达的比较研究。

IF 2 4区农林科学

Experimental Animals Pub Date : 2026-07-01 Epub Date: 2026-02-24 DOI: 10.1538/expanim.25-0084

Siquan Liao, Dandan Peng, Bing Chao, Fang Deng, Bu He, Jianping Zhang

{"title":"Comparative study of renal filtration barrier structure and key gene expression between Lepus yarkandensis and Oryctolagus cuniculus.","authors":"Siquan Liao, Dandan Peng, Bing Chao, Fang Deng, Bu He, Jianping Zhang","doi":"10.1538/expanim.25-0084","DOIUrl":"10.1538/expanim.25-0084","url":null,"abstract":"The Lepusyarkandensis (L. yarkandensis) is an endemic species inhabiting the arid ecosystem of the Tarim Basin, characterized by extreme dryness and scarce water resources. In contrast, Oryctolagus cuniculus (O. cuniculus) is adapted to temperate environments rich in water. Over the course of long-term evolution, the L. yarkandensis has likely developed unique physiological and histological adaptations to cope with the survival challenges posed by such arid conditions. This study aims to investigate the differences between the L. yarkandensis and O. cuniculus in terms of hematological and biochemical blood parameters, renal histology, water-salt regulation capacity, and the expression of glomerular filtration barrier proteins, in order to investigate the adaptive strategies of the L. yarkandensis to drought environments. The results showed that the L. yarkandensis exhibited higher red blood cell counts, hematocrit levels, urine osmolality, and total urinary protein levels compared to O. cuniculus, while the urine pH was lower. Histological analysis revealed increased collagen content in the outer medulla and more complex tubular structures in the kidneys of the L. yarkandensis. Molecular analysis further demonstrated upregulated expression of glomerular filtration barrier proteins nephrin, podocin, and CD2AP, alongside downregulated expression of laminin and WT1. In summary, these findings provide insights into the potential physiological and molecular adaptations of L. yarkandensis, laying the groundwork for future research into the evolution of desert fauna.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"306-321"},"PeriodicalIF":2.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13349739/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147276022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Repeated surgical oocyte collection from severe immunodeficient NOG mice after repeated superovulation with inhibin antiserum and eCG. 用抑制素抗血清和心电图反复采集重度免疫缺陷NOG小鼠多次超排卵后的手术卵母细胞。

IF 2 4区农林科学

Experimental Animals Pub Date : 2026-07-01 Epub Date: 2026-02-05 DOI: 10.1538/expanim.24-0140

Kumiko Takezawa, Motohito Goto, Kanako Oda, Nae Saito, Toshikuni Sasaoka, Riichi Takahashi

{"title":"Repeated surgical oocyte collection from severe immunodeficient NOG mice after repeated superovulation with inhibin antiserum and eCG.","authors":"Kumiko Takezawa, Motohito Goto, Kanako Oda, Nae Saito, Toshikuni Sasaoka, Riichi Takahashi","doi":"10.1538/expanim.24-0140","DOIUrl":"10.1538/expanim.24-0140","url":null,"abstract":"Repeated surgical oocyte collection in mice enables the harvest of multiple cohorts of eggs from a single female, thereby reducing the need for animal use. Traditionally, oocyte collection for the mouse requires euthanasia, and only two sequential collections have been reported. The most efficient superovulation protocol involves the use of inhibin antiserum; however, repeated administration may pose a risk of anaphylactic reactions. In this study, we used severely immunodeficient NOG mice to evaluate the feasibility of performing three serial oocyte collections with inhibin antiserum treatments. We found that three consecutive cycles of antiserum administration followed by surgical retrieval cycles were achieved without mortality. Although the number of mice from which oocytes could be collected decreased over successive cycles, oocyte retrieval remained feasible. The application of the anti-adhesion film improved the number of mice from which oocytes could be recovered. Furthermore, embryos derived from the third retrieval developed to term after transfer. However, compared with the control group, we were unable to achieve a proportional increase in the number of retrieved oocytes corresponding to the number of collection cycles. Further improvements to this repeated collection method may include increasing the number of consecutive retrievals, optimizing the protocol to enhance both the oocytes yield and the fertilization rates. Such advances could increase oocyte yield per female, enable the mass production of embryos from fewer animals.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"288-295"},"PeriodicalIF":2.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13349741/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146117909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Honghe Fujie lotion ameliorates pregnancy-associated vulvovaginal candidiasis in mice by inhibiting the activation of NLRP3 inflammasome. 红河扶洁洗剂通过抑制NLRP3炎性体的激活改善妊娠相关小鼠外阴阴道念珠菌病。

IF 2 4区农林科学

Experimental Animals Pub Date : 2026-07-01 Epub Date: 2026-04-10 DOI: 10.1538/expanim.25-0130

Ying Wang, Yiqun Jiang, Simao Shi, Yu Wang, Jiyu Yang, Hongying Kuang, Xiaoling Feng

{"title":"Honghe Fujie lotion ameliorates pregnancy-associated vulvovaginal candidiasis in mice by inhibiting the activation of NLRP3 inflammasome.","authors":"Ying Wang, Yiqun Jiang, Simao Shi, Yu Wang, Jiyu Yang, Hongying Kuang, Xiaoling Feng","doi":"10.1538/expanim.25-0130","DOIUrl":"10.1538/expanim.25-0130","url":null,"abstract":"Vulvovaginal candidiasis, a common infection of the female reproductive tract, poses heightened risks when occurring during pregnancy. Although Honghe Fujie lotion (HFL), a traditional Chinese medicine, is clinically used for mycotic vaginitis, its therapeutic efficacy and mechanisms in pregnancy-associated vulvovaginal candidiasis (pVVC) remain unclear. In this study, HPLC-MS was used to analyze and identify the compounds in HFL. This study investigated HFL's effects through in vitro and in vivo approaches. A murine model of vaginal Candida albicans (C. albicans) infection was established to evaluate the therapeutic effect of HFL on pVVC. Vaginal fungal load was quantified by analyzing vaginal lavage fluid, while the impact of HFL on adverse pregnancy outcomes was assessed through placental developmental analysis. Furthermore, the effects of HFL on pVVC-induced vaginitis and NLRP3 inflammasome activation were examined using immunofluorescence, enzyme-linked immunosorbent assay, and western blot. The results included: (1) High performance liquid chromatography coupled with mass spectrometry (HPLC-MS) analysis identified 545 chemical constituents in HFL. (2) HFL significantly inhibited C. albicans growth and biofilm metabolic activity in vitro; (3) in a murine pVVC model, HFL reduced vaginal fungal load and mitigated adverse pregnancy outcomes, while also restoring trophoblast cell distribution in placental tissues; (4) mechanistically, HFL suppressed NLRP3 inflammasome activation, leading to Caspase-1 activity downregulation and subsequent impairment of pro-inflammatory cytokine secretion. These findings demonstrate that HFL alleviates pVVC by targeting the NLRP3 signaling pathway, providing a preclinical foundation for its therapeutic application in pregnancy-associated VVC.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"343-356"},"PeriodicalIF":2.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13349744/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147671852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of sex hormones on the development of diabetes and pancreatic islet gene expression in obese type 2 diabetes model ZFDM rat. 性激素对肥胖2型糖尿病模型ZFDM大鼠糖尿病发生及胰岛基因表达的影响

IF 2 4区农林科学

Experimental Animals Pub Date : 2026-07-01 Epub Date: 2026-01-17 DOI: 10.1538/expanim.25-0122

Norihide Yokoi, Saki Shigenaka, Chiaki Nakata, Misato Takagi, Yuting Xiao, Tomoki Hirokoji, Tomohiko Sasase, Kinuko Uno, Takeshi Ohta

{"title":"Effects of sex hormones on the development of diabetes and pancreatic islet gene expression in obese type 2 diabetes model ZFDM rat.","authors":"Norihide Yokoi, Saki Shigenaka, Chiaki Nakata, Misato Takagi, Yuting Xiao, Tomoki Hirokoji, Tomohiko Sasase, Kinuko Uno, Takeshi Ohta","doi":"10.1538/expanim.25-0122","DOIUrl":"10.1538/expanim.25-0122","url":null,"abstract":"Sex differences have been observed in the onset of type 2 diabetes, with men being more likely to develop the disease than women. There is a clear sex difference in the development of diabetes in obese type 2 diabetes model ZFDM rats, with all males developing diabetes, but none of the females. In the present study, we examined the effects of sex hormones on the development of diabetes and gene expression in the pancreatic islets of ZFDM rats. All experiments were performed using 6-week-old ZFDM rats homozygous for the leptin receptor mutation (fatty, fa). Orchiectomy did not affect the development of diabetes but slightly suppressed non-fasting blood glucose levels in male ZFDM rats. Except for one diabetic rat, ovariectomy did not affect non-fasting blood glucose levels but impaired glucose tolerance in female ZFDM rats, which was accompanied by accelerated islet fibrosis and pancreatic β-cell loss. Administration of the female hormone (17β-estradiol, E2) did suppress the development of diabetes in male ZFDM rats. Pair feeding matched with the E2-administered group also suppressed the development of diabetes in male ZFDM rats. Direct E2 treatment of the pancreatic islets of diabetic male ZFDM rats changed the expression of genes related to type 2 diabetes and insulin secretion closer to those of obese non-diabetic male ZF rats. These results suggest that female hormones have the suppressive effects on the development of diabetes, whereas male hormones, if any, have the opposite effects in ZFDM rats.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"262-276"},"PeriodicalIF":2.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13349738/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145997702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0