Experimental Animals最新文献_第4页

Disruption of intestinal barrier and dysbiosis of gut microbiota in an experimental rhesus macaque model with 6-year diabetes mellitus. 6年糖尿病恒河猴模型的肠道屏障破坏和肠道菌群失调。

IF 2.2 4区农林科学

Experimental Animals Pub Date : 2025-01-17 DOI: 10.1538/expanim.24-0125

Xiangyu Fu, Xiang Ren, Maoyuan Zhao, Lan Li, Yaojia Zhou, Yanrong Lu, Chengshi Wang

{"title":"Disruption of intestinal barrier and dysbiosis of gut microbiota in an experimental rhesus macaque model with 6-year diabetes mellitus.","authors":"Xiangyu Fu, Xiang Ren, Maoyuan Zhao, Lan Li, Yaojia Zhou, Yanrong Lu, Chengshi Wang","doi":"10.1538/expanim.24-0125","DOIUrl":"https://doi.org/10.1538/expanim.24-0125","url":null,"abstract":"This study aims to clarify the disruption of gut barrier and dysbiosis of the microbiota in an experimental macaque model with 6-year diabetes mellitus (DM), and provide evidence for the application of therapeutic strategies targeting the human microbiota in the future. A single intravenous injection of high-dose streptozotocin was used to induce the type 1 diabetes (T1D) macaque model. Hematoxylin-Eosin (HE) and Periodic Acid Schiff (PAS) staining were conducted to observe colon morphological changes. The composition of gut microbiota was detected using 16S rRNA gene sequencing, and bioinformatics analysis was adopted to predict alterations in the microbial phenotype and function. Obvious intestinal inflammation and decreased goblet cells were observed in T1D macaques. 16S rRNA gene sequencing suggested a significantly different β diversity of the microbiota in the T1D group, where expanded Proteobacteria (dominantly Escherichia-Shigella) and Actinomycetota (formerly known as Actinobacteria) replaced the dominance of Bacillota (formerly known as Firmicutes) and Bacteroidota (formerly known as Bacteroidetes), indicating an imbalance in the microbial composition. Archaea was identified as a biomarker between groups. Moreover, with the reduction of beneficial bacteria (Lactobacillaceae) and the increase of pro-inflammatory bacteria and opportunistic pathogens (Enterobacteriaceae), the phenotypes of the microbiota were reversed, resulting in abnormal up- (e.g., carbohydrate and amino acid metabolism) or down-regulation (e.g., protein digestion and absorption) of multiple metabolic pathways. There were intestinal structural disorders and gut microbiota dysbiosis in T1D macaques, indicating that strategies targeting gut microbiota may be effective to treat metabolic diseases like DM.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143002798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Trophectoderm-specific gene manipulation using adeno-associated viral vectors. 利用腺相关病毒载体进行滋养外胚层特异性基因操作。

IF 2.2 4区农林科学

Experimental Animals Pub Date : 2025-01-11 DOI: 10.1538/expanim.24-0165

Tatsuya Nakagawa, Chihiro Emori, Masahito Ikawa

{"title":"Trophectoderm-specific gene manipulation using adeno-associated viral vectors.","authors":"Tatsuya Nakagawa, Chihiro Emori, Masahito Ikawa","doi":"10.1538/expanim.24-0165","DOIUrl":"https://doi.org/10.1538/expanim.24-0165","url":null,"abstract":"In mammals, blastocyst-stage trophectoderm (TE) contacts the maternal body at the time of implantation and forms the placenta after implantation, which supports the development of the fetus. Studying gene function in TE and placenta is important to understand normal implantation and pregnancy processes and their dysfunction. However, genetically modified mice are commonly generated by manipulating pronuclear-stage zygotes, which modify both the genome of the fetus and the placenta. Therefore, we previously developed TE/placenta-specific gene expression technology by transducing blastocysts with lentiviral vectors. However, the zona pellucida (ZP) needed to be removed before transduction. In this study, we examined various adeno-associated viral (AAV) vectors to develop a new TE/placenta-specific gene transduction method. As AAV1 can path through ZP, we succeeded in trophoblast-specific gene expression without ZP removal. Furthermore, TE cells genetically modified by AAV1-Cre contributed uniformly to the placenta. Our new technology contributes to advances in implantation and placenta research and leads to the development of new assisted reproductive technology.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142978096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mouse NOD/Shi and NSY/Hos strains infected with Plasmodium berghei ANKA are models for experimental cerebral malaria. 小鼠 NOD/Shi 和 NSY/Hos 株感染了伯格氏疟原虫 ANKA，是实验性脑疟疾的模型。

IF 2.2 4区农林科学

Experimental Animals Pub Date : 2025-01-10 Epub Date: 2024-07-26 DOI: 10.1538/expanim.24-0023

Tamio Ohno, Nozomi Iwatake, Yuki Miyasaka

{"title":"Mouse NOD/Shi and NSY/Hos strains infected with Plasmodium berghei ANKA are models for experimental cerebral malaria.","authors":"Tamio Ohno, Nozomi Iwatake, Yuki Miyasaka","doi":"10.1538/expanim.24-0023","DOIUrl":"10.1538/expanim.24-0023","url":null,"abstract":"In humans, cerebral malaria is the most common cause of malaria-related mortality. Mouse C57BL/6 (B6) sub-strains are the major model system for experimental cerebral malaria (ECM) as they show similar pathophysiology to human cerebral malaria after infection with the rodent malaria parasite Plasmodium berghei ANKA. This model system has been used to analyze the molecular mechanisms of cerebral malaria. To develop new mouse models, we analyzed the ECM susceptibility of NOD/Shi (NOD) and NSY/Hos (NSY) strains established from the non-inbred ICR strain. Both NOD and NSY strains exhibited clinical symptoms and pathologies similar to ECM in C57BL/6J (B6J) mice and died within 11 days of infection. Thus, the NOD and NSY strains are susceptible to ECM and may be useful as new ECM models. The ECM susceptibility of both strains is suggested to be due to homozygosity for the cerebral malaria susceptibility allele of the ECM susceptible ICR strain. Although analyses using B6 sub-strains have proposed that complement component 5 (C5) plays an important role in ECM pathogenesis, we found that C5 was not essential as the ECM susceptible NOD strain is C5 deficient. Thus, results obtained from B6 sub-strains may not reflect the full picture of ECM in mice. Comparative analyses of multiple ECM models will contribute to a more accurate identification of the factors essential for ECM.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"31-38"},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11742482/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141787662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dosage and organic acid residue of myelin oligodendrocyte glycoprotein_35-55 peptide influences immunopathology and development of Bacillus Calmette-Guérin induced experimental autoimmune encephalomyelitis. MOG35-55肽的剂量和有机酸残留对卡介苗诱导的实验性自身免疫性脑脊髓炎（EAE）的免疫病理和发展的影响

IF 2.2 4区农林科学

Experimental Animals Pub Date : 2025-01-10 Epub Date: 2024-07-10 DOI: 10.1538/expanim.24-0012

Xiaoyan Han, Ying Wang, Kehua Zhang, Tao Na, Tingting Wu, Xiaofang Hao, Yuxuan Jin, Yuchun Wang, Haohan Wang, Shufang Meng

{"title":"Dosage and organic acid residue of myelin oligodendrocyte glycoprotein35-55 peptide influences immunopathology and development of Bacillus Calmette-Guérin induced experimental autoimmune encephalomyelitis.","authors":"Xiaoyan Han, Ying Wang, Kehua Zhang, Tao Na, Tingting Wu, Xiaofang Hao, Yuxuan Jin, Yuchun Wang, Haohan Wang, Shufang Meng","doi":"10.1538/expanim.24-0012","DOIUrl":"10.1538/expanim.24-0012","url":null,"abstract":"Experimental autoimmune encephalomyelitis (EAE) serves as a model for studying multiple sclerosis, with immunization strategies utilizing myelin oligodendrocyte glycoprotein (MOG)35-55 peptide, emulsified in adjuvant enriched with Mycobacterium tuberculosis (Mtb). This study examined the effects of Bacillus Calmette-Guérin (BCG) as an adjuvant, alongside the impact of MOG35-55 peptide doses and their residual counter ions on EAE development. We found that BCG can be effectively used to induce EAE with similar incidence and severity as heat-killed H37Ra, contingent upon the appropriate MOG35-55 peptide dose. Different immunization doses of MOG35-55 peptide significantly affect EAE development, with higher doses leading to a paradoxical reduction in disease activity, probably due to peripheral tolerance mechanisms. Furthermore, doses of MOG35-55 peptides with acetate showed a more pronounced effect on disease development compared to those containing trifluoroacetic acid (TFA), suggesting the potential influence of residual counter ions on EAE activity. We highlighted the feasibility of applying BCG to the establishment of EAE for the first time. Our findings emphasized the importance of MOG35-55 peptide dosage and composition in modulating EAE development, offering insights into the mechanisms of autoimmunity and tolerance. This could have implications for autoimmune disease research and the design of therapeutic strategies.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"16-30"},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11742474/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141579361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of inhaled anesthesia for Octodon degus using electroencephalography. 利用脑电图优化八爪鱼的吸入麻醉。

IF 2.2 4区农林科学

Experimental Animals Pub Date : 2025-01-10 Epub Date: 2024-08-22 DOI: 10.1538/expanim.24-0017

Yuki Ikai, Goro A Nagura-Kato, Shinsuke H Sakamoto, Akio Shinohara, Chihiro Koshimoto

{"title":"Optimization of inhaled anesthesia for Octodon degus using electroencephalography.","authors":"Yuki Ikai, Goro A Nagura-Kato, Shinsuke H Sakamoto, Akio Shinohara, Chihiro Koshimoto","doi":"10.1538/expanim.24-0017","DOIUrl":"10.1538/expanim.24-0017","url":null,"abstract":"Physiological responses to inhaled anesthetics vary among species. Therefore, a precise anesthetic technique is important for each individual species. In this study, we focused on the degu (Octodon degus), a small herbivorous rodent. Degus have recently begun to be used as laboratory models for brain research because of certain human-like characteristics, such as spontaneous development of Alzheimer's disease. In this study, we evaluated appropriate induction and maintenance anesthesia conditions for isoflurane and sevoflurane in degus by a stimulation test, electroencephalography (EEG), minimum alveolar concentration (MAC), and vital signs. During induction, more rapid time to loss of the righting reflex and deeper anesthesia in degus were observed in isoflurane. The MAC value for degus were 1.75 ± 0.0% in isoflurane and 2.25 ± 0.27% in sevoflurane. Whereas some degus were awake during maintenance anesthesia using both anesthetics at concentrations of ≤2%, no rats were awake when using sevoflurane at a concentration of 2%. The duration of the total flat EEG, a measure of the depth of maintenance anesthesia, was longer for isoflurane than for sevoflurane. Furthermore, higher concentrations of both anesthetics suppressed the respiratory rate in degus. These new findings regarding inhalation anesthesia in degus will contribute to future developments in the fields of laboratory animals and veterinary medicine.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"93-103"},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11742480/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142016861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hematological and biochemical characterization of aging farm male rat strains in the national center for geriatrics and gerontology. 国家老年医学和老年学中心的老龄化农场雄性大鼠品系的血液学和生物化学特征。

IF 2.2 4区农林科学

Experimental Animals Pub Date : 2025-01-10 Epub Date: 2024-08-08 DOI: 10.1538/expanim.24-0028

Julio A Almunia, Yoshiko Munesue, Haruka Kawasaki, Kazumichi Takano, Chisato Kayahara, Satoko Noma, Nobuko Morikawa, Shumpei Niida, Noboru Ogiso

{"title":"Hematological and biochemical characterization of aging farm male rat strains in the national center for geriatrics and gerontology.","authors":"Julio A Almunia, Yoshiko Munesue, Haruka Kawasaki, Kazumichi Takano, Chisato Kayahara, Satoko Noma, Nobuko Morikawa, Shumpei Niida, Noboru Ogiso","doi":"10.1538/expanim.24-0028","DOIUrl":"10.1538/expanim.24-0028","url":null,"abstract":"Laboratory rats, like mice, are a type of animal commonly used in scientific investigations as well as in basic aging and geriatric research. The selection of a rat strain is an important first step in the planning and design of an experiment due to physiological, anatomical, and ethological variations in each strain, which may significantly modify the expected results. In the present study, we characterized age-related changes, from 3 months old (mo) to 24 mo, in three male rat strains commonly used in medical research: RccHan®️:WIST (RccHan:WIST), F344/NSlc (F344), and Slc:SD Rat (SD). The body weight, water/food consumption, and survival rate of each strain were physiologically evaluated. Hematological and biochemical values were analyzed every three months. Hematological results showed a decrease in lymphocytes and increases in other leukocytes from 12 mo in F344 and SD rats. The incidence of hematological disorder was 10-15% in F344 and SD rats from 18 mo. Increases in hepatic biochemical parameters (alanine transaminase (GPT/ALT) and aspartate transaminase (GOT/AST)) and cytopathological parameters (creatine phosphokinase (CPK)) were observed in male F344 rats at 12 mo. Triglycerides (TG) serum levels were significantly elevated in the 12 mo RccHan:WIST rats, while Lipase (LIP) levels were significantly reduced in 24 mo. The present results revealed significant variations in hematological and biochemical values in the different laboratory male rat strains due to genetic and nutritional-metabolic factors specific to each strain.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"66-82"},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11742475/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141901411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibition of tumor necrosis factor receptor-associated factor 6 alleviates secondary brain injury by reducing neuronal pyroptosis after intracerebral hemorrhage. 抑制 TRAF6 可减少脑出血后神经元的脓毒症，从而减轻继发性脑损伤。

IF 2.2 4区农林科学

Experimental Animals Pub Date : 2025-01-10 Epub Date: 2024-08-07 DOI: 10.1538/expanim.24-0078

Qianxin Hu, Haixin Zeng, Chengao Feng, Wei Tian, Yuxin He, Bing Li

{"title":"Inhibition of tumor necrosis factor receptor-associated factor 6 alleviates secondary brain injury by reducing neuronal pyroptosis after intracerebral hemorrhage.","authors":"Qianxin Hu, Haixin Zeng, Chengao Feng, Wei Tian, Yuxin He, Bing Li","doi":"10.1538/expanim.24-0078","DOIUrl":"10.1538/expanim.24-0078","url":null,"abstract":"Secondary brain injury (SBI) is one of the main causes of high mortality and disability rates following intracerebral hemorrhage (ICH). Tumor necrosis factor receptor-associated factor 6 (TRAF6) plays a crucial role in the process of pyroptosis, and modulating its expression may present a novel therapeutic strategy for mitigating brain injury. This study aims to explore the mechanisms of TRAF6 in pyroptosis after ICH. C57BL/6J mice were used to establish the ICH model. Brain was collected at different time points for q-PCR and western blot to detect the level of TRAF6. After the C25-140 (the TRAF6 inhibitor) was administrated, the mice were divided into four groups. Then, the neurological deficit, brain water content, and blood-brain barrier (BBB) damage were detected. Immunofluorescence and western blot were used to detect the level of pyroptosis proteins, and ELISA and q-PCR were used to detect the levels of IL-18 and IL-1β. TRAF6 expression was upregulated after ICH and was mainly expressed in neurons. Inhibition of TRAF6 expression with C25-140 alleviated neurological deficits and reduced brain edema after ICH. In addition, inhibition of TRAF6 also reduced the expression of pyroptosis inflammasomes such as GSDMD, NLRP3, and ASC, as well as neurological damage caused by IL-18 and IL-1β after ICH. TRAF6 regulates neuronal pyroptosis in SBI after ICH. Inhibition of TRAF6 may be a potential target for alleviating inflammatory damage after ICH.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"39-48"},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11742478/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141901412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Endothelial GATA3 is involved in coagulofibrinolytic homeostasis during endotoxin sepsis. 内皮 GATA3 参与了内毒素败血症期间的凝血纤维蛋白溶解平衡。

IF 2.2 4区农林科学

Experimental Animals Pub Date : 2025-01-10 Epub Date: 2024-09-04 DOI: 10.1538/expanim.24-0079

Moyuru Hayashi, Shin'ya Ohmori, Yoshiko Kawai, Takashi Moriguchi

{"title":"Endothelial GATA3 is involved in coagulofibrinolytic homeostasis during endotoxin sepsis.","authors":"Moyuru Hayashi, Shin'ya Ohmori, Yoshiko Kawai, Takashi Moriguchi","doi":"10.1538/expanim.24-0079","DOIUrl":"10.1538/expanim.24-0079","url":null,"abstract":"Sepsis-induced acute lung injury represents a significant threat to human health and is frequently associated with pulmonary thrombosis due to dysregulation of the coagulofibrinolytic system. Plasmin, the major protease that degrades fibrin aggregates, is activated predominantly by tissue-plasminogen activator (tPA), whereas tPA is negatively regulated by plasminogen activator inhibitor (PAI-1). Under septic conditions, the imbalance between coagulation and fibrinolysis results in excessive microthrombosis. Pulmonary capillary endothelial cells serve as a primary source of tPA and PAI-1. The molecular pathways regulating their expression levels depend on the differential activity of transcription factors. In this study, we elucidated the role of the zinc-finger transcription factor GATA3 in response to sepsis-induced pulmonary embolism. Endothelial cell-specific GATA3-deficient mice (G3-ECKO) presented increased susceptibility to bacterial endotoxin-induced pulmonary embolism, which was associated with increased PAI-1 expression levels and decreased tPA expression levels in the lungs. Septic lung extracts from G3-ECKO mice consistently presented decreased plasmin activity, which likely underlies the increased coagulation. These results demonstrate that GATA3 plays a protective role against bacterial endotoxin-induced pulmonary vascular embolism. Our findings will contribute to understanding the molecular mechanisms involving GATA3 in preventing pulmonary embolism.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"104-113"},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11742476/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142132233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of gene mutations associated with the phenotype of short-limb mice emerging from a foundation colony of severely immunodeficient mice. 鉴定与严重免疫缺陷小鼠基础群中出现的短肢小鼠表型相关的基因突变。

IF 2.2 4区农林科学

Experimental Animals Pub Date : 2025-01-10 Epub Date: 2024-09-11 DOI: 10.1538/expanim.24-0045

Yui Kaneko, Kayo Tomiyama, Masahiko Yasuda, Yuji Komaki, Tomoyuki Ogura, Riichi Takahashi, Masafumi Yamamoto

{"title":"Identification of gene mutations associated with the phenotype of short-limb mice emerging from a foundation colony of severely immunodeficient mice.","authors":"Yui Kaneko, Kayo Tomiyama, Masahiko Yasuda, Yuji Komaki, Tomoyuki Ogura, Riichi Takahashi, Masafumi Yamamoto","doi":"10.1538/expanim.24-0045","DOIUrl":"10.1538/expanim.24-0045","url":null,"abstract":"Here, we report the identification of causative genes for limb-shortening in individuals repeatedly found in a population of severely immunodeficient NOG mice maintained via sibling mating. First, we conducted a pedigree survey to determine whether limb-shortening was a recessive genetic trait and then identified it using a crossing test. Simultaneously, the symptoms were identified in detail using pathological analysis. Accordingly, a mouse strain exhibiting a recessive trait caused by a single gene trait and similar symptoms was identified, suggesting growth differentiation factor 5 (Gdf5) as a causative gene. Genome walking via PCR and sequence analysis of Gdf5 revealed a deletion of approximately 1.1 kb from the latter half of exon 2 of Gdf5. Furthermore, we established NOG-Gdf5bpJic by removing other modified genes and confirmed that the inheritance pattern was reconfirmed semi-dominant. In recent years, regenerative medicine research using immunodeficient mice has been actively conducted, and this murine strain is expected to contribute to niche stem cell analysis and transplantation research.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"122-131"},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11742472/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142282552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Chinese hamster as an excellent experimental animal model. 中国仓鼠是一种优秀的实验动物模型。

IF 2.2 4区农林科学

Experimental Animals Pub Date : 2025-01-10 Epub Date: 2024-08-28 DOI: 10.1538/expanim.24-0029

Xiaoqi Chang, Jiping Gao, Junting Yang, Yunhui Ma, Guohua Song

{"title":"The Chinese hamster as an excellent experimental animal model.","authors":"Xiaoqi Chang, Jiping Gao, Junting Yang, Yunhui Ma, Guohua Song","doi":"10.1538/expanim.24-0029","DOIUrl":"10.1538/expanim.24-0029","url":null,"abstract":"Hamsters are valuable rodent models that are distinct from mice and rats. Currently, the main hamster species used for experimental research are the Syrian golden hamster and Chinese hamster, in addition to hamster species from other countries. Chinese hamsters are small, easy to run and feed, and inexpensive. They are prominent species found only in China and are part of the experimental animal resources of Chinese specialty. Chinese hamsters are distinguished by a black stripe on their back, short tail, pair of easily retractable cheek pouches, and pair of large drooping testes in males with 22 chromosomes. Due to their unique anatomical structure and biological features, Chinese hamsters have been used as a model in biomedical research. Moreover, the breeding and use of Chinese hamsters was comprehensively studied in 1958, with significant breakthroughs. We present a thorough review of the current developments and applications of Chinese hamsters and support the use of this species as a suitable and innovative experimental research model. With the success of Chinese hamster transgenic technology, this species will become more commonly employed in biological and medical research in the future.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"1-15"},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11742471/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142092562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0