Experimental Animals最新文献

Generation of mice expressing liver-specific fluorescent genes and the optimal conditions for signal detection via in vivo imaging. 表达肝脏特异性荧光基因的小鼠的产生和通过体内成像检测信号的最佳条件。

IF 1.2 4区农林科学

Experimental Animals Pub Date : 2025-09-25 DOI: 10.1538/expanim.25-0092

Shuho Hori, Hideki Hayashi, Kayoko Iwao, Ayaka Nakamura, Hideaki Sumiyoshi, Yutaka Inagaki, Masato Ohtsuka, Hiromi Miura

{"title":"Generation of mice expressing liver-specific fluorescent genes and the optimal conditions for signal detection via in vivo imaging.","authors":"Shuho Hori, Hideki Hayashi, Kayoko Iwao, Ayaka Nakamura, Hideaki Sumiyoshi, Yutaka Inagaki, Masato Ohtsuka, Hiromi Miura","doi":"10.1538/expanim.25-0092","DOIUrl":"https://doi.org/10.1538/expanim.25-0092","url":null,"abstract":"In vivo imaging enables real-time detection of excitation and emission signals and is useful for the noninvasive evaluation of temporal changes in biological tissues. The near-infrared fluorescent protein iRFP can be used for deep-tissue imaging because it emits light at wavelengths that are less attenuated by biological tissues. However, autofluorescence originating from diet, tissues, and the imaging environment can interfere with fluorescence detection; therefore, appropriate animal pretreatment and optimization of imaging conditions are essential. We generated two mouse strains: AlbeGiR reporter mice, in which enhanced green fluorescent protein (eGFP) and iRFP713 genes were tandemly inserted downstream of the Albumin gene, and hairless mice (HrΔ164/Δ164), carrying a mutation in the hairless gene. Their offspring were used in in vivo imaging experiments to investigate: (i) the localization of eGFP and iRFP713 fluorescence, (ii) the influence of hair on fluorescence detection, and (iii) suitable filter combinations for fluorescence detection. In the resulting mice, liver-specific expression of both eGFP and iRFP713 was observed at the same anatomical location. Although autofluorescence was more prominent in hairless mice than in furred mice, signal detection was improved either by using longer-wavelength excitation/emission filters or by applying spectral unmixing to separate the target signal. These findings provide practical guidance for optimizing in vivo fluorescence imaging conditions using standard IVIS platforms.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145137030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Educational efficacy of training videos and simulators for teaching basic mouse experimental skills to novice veterinary students. 训练视频及模拟器对兽医学新手小鼠基本实验技能的教学效果。

IF 1.2 4区农林科学

Experimental Animals Pub Date : 2025-09-20 DOI: 10.1538/expanim.25-0093

Atsushi Tsukamoto, Thum Su Zan, Makie Nitta, Hiromitsu Yoshida, Hirotaka Katahira, Yoshiharu Fujita, Satoshi Takagi, Shinichiro Nakamura

{"title":"Educational efficacy of training videos and simulators for teaching basic mouse experimental skills to novice veterinary students.","authors":"Atsushi Tsukamoto, Thum Su Zan, Makie Nitta, Hiromitsu Yoshida, Hirotaka Katahira, Yoshiharu Fujita, Satoshi Takagi, Shinichiro Nakamura","doi":"10.1538/expanim.25-0093","DOIUrl":"https://doi.org/10.1538/expanim.25-0093","url":null,"abstract":"Alternative educational tools, such as training videos and simulators, are recommended in the education of laboratory animal science. However, evidence supporting their educational utility in the training of rodent experimental techniques remains limited. In this study, we assessed the utility of alternative educational tools in the practice of laboratory animal science for novice veterinary students. 149 students participated in a stepwise program beginning with lectures, followed by preparatory learning sessions using training videos and two types of mouse simulators (a silicone-based model and fabric toy mouse), and then hands-on training with live mice. The program covered basic techniques: habituation, restraint, and vaginal smear sampling for estrous cycle determination. A survey-based evaluation was conducted to assess the educational utility of alternative educational tools. The contribution of each preparatory resource (videos, lectures, simulators, printed materials, and notes) to skill acquisition was evaluated, showing that videos, lectures, and printed materials highly contributed. The training videos were rated as more necessary than the simulators for skill acquisition. Psychological evaluation showed that 84% of students experienced anxiety before practice. A positive correlation was found between anxiety levels and frequency of use for all three tools, and students reported that all tools were effective in reducing anxiety during practice. All techniques showed high proficiency rates. Our findings suggest that integrating alternative tools with live-animal training promotes technical skill acquisition, enhances psychological readiness, and supports 3Rs-based laboratory animal practice.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145112288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

D-galactose treatment accumulates AGEs but induces no further detrimental effects in HR-1 mouse skin. d -半乳糖处理会累积AGEs，但不会对HR-1小鼠皮肤产生进一步的有害影响。

IF 1.2 4区农林科学

Experimental Animals Pub Date : 2025-09-05 DOI: 10.1538/expanim.25-0026

Mako Isemura, Ryoga Kinosita, Sakura Hattori, Karina Kouzaki, Yuki Tamura, Hiroya Urabe, Hiroyuki Uno, Ryuji Akimoto, Koichi Nakazato

{"title":"D-galactose treatment accumulates AGEs but induces no further detrimental effects in HR-1 mouse skin.","authors":"Mako Isemura, Ryoga Kinosita, Sakura Hattori, Karina Kouzaki, Yuki Tamura, Hiroya Urabe, Hiroyuki Uno, Ryuji Akimoto, Koichi Nakazato","doi":"10.1538/expanim.25-0026","DOIUrl":"https://doi.org/10.1538/expanim.25-0026","url":null,"abstract":"As aging affects the appearance of the skin, anti-aging research has intensified in dermatology, skincare, and aesthetic medicine. Because natural aging takes a very long time, one essential anti-aging approach is to pharmacologically mimic aging, such as with D-galactose treatment. Hairless mice (HR-1) have been extensively used in skin research because of their lack of body hair and ease of animal care. In the present study, HR-1 mice were treated with D-galactose to determine whether detrimental effects were induced in the skin. After 3 months of D-galactose treatment, AGEs in the skin significantly increased. On the other hand, no signs of skin disorders (dermal thickness, type I collagen content, expression of various genes, collagen synthesis, and degradation signals) were observed. Even when the concentration of D-galactose increased, no apparent changes in dermal thickness were observed. These findings suggest that D-galactose treatment induces AGEs accumulation but no further detrimental effects in the HR-1 skin.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145014185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rat polyomavirus 2 infection - secondary publication. 大鼠多瘤病毒2型感染-继发出版物。

IF 1.2 4区农林科学

Experimental Animals Pub Date : 2025-09-03 DOI: 10.1538/expanim.25-0072

Miyuu Tanaka

{"title":"Rat polyomavirus 2 infection - secondary publication.","authors":"Miyuu Tanaka","doi":"10.1538/expanim.25-0072","DOIUrl":"https://doi.org/10.1538/expanim.25-0072","url":null,"abstract":"In 2016, an outbreak of Rattus norvegicus polyomavirus 2 (RatPyV2) infection was reported in a colony of X-linked severe combined immunodeficiency (XSCID) rats in the United States. While RatPyV2 infection persists asymptomatically in immunocompetent rats, immunodeficient XSCID rats develop variable respiratory symptoms, emaciation, impaired breeding performance, and systemic deteriorating condition. RatPyV2 is an epitheliotropic virus targeting epithelial cells of the salivary glands, Harderian glands, extraorbital lacrimal glands, respiratory system, and reproductive or accessory reproductive organs. Histopathologically, the formation of large basophilic nuclear inclusion bodies in the infected epithelial cells is a characteristic feature, along with hyperplasia or dysplasia. Glandular atrophy and loss, accompanied by fibrosis and mononuclear cell infiltration, are also observed in the salivary glands, Harderian glands, and extraocular lacrimal glands. In particular, the parotid salivary glands are prone to be severely and extensively affected with relatively severe and diffuse lesions even at one month of age. Severely affected animals also develop interstitial pneumonia. Among target tissues, the parotid salivary glands appear to be higher susceptible to RatPyV2, therefore pathological examination and PCR examination of the salivary glands, including the parotid salivary glands, are essential for the diagnosis of RatPyV2 infection. This review paper provides a comprehensive summary of the features (clinical signs, pathological findings, and transmission), diagnostic methods, and prevalence of RatPyV2 infection, based on our research and reports from research groups in the United States.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144992006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Early-stage therapeutic efficacy of TNAP inhibition using a novel milder murine model of CKD-MBD. 用一种新型轻度CKD-MBD小鼠模型研究TNAP抑制的早期治疗效果。

IF 1.2 4区农林科学

Experimental Animals Pub Date : 2025-09-02 DOI: 10.1538/expanim.25-0065

Kaori Soma, José Luis Millán, Anthony Pinkerton, Masanori Izumi

{"title":"Early-stage therapeutic efficacy of TNAP inhibition using a novel milder murine model of CKD-MBD.","authors":"Kaori Soma, José Luis Millán, Anthony Pinkerton, Masanori Izumi","doi":"10.1538/expanim.25-0065","DOIUrl":"https://doi.org/10.1538/expanim.25-0065","url":null,"abstract":"Chronic kidney disease (CKD) is a complicated systemic disease displaying various pathophysiological symptoms including mineral bone disorder (CKD-MBD). Ideally, early intervention for CKD-MBD would be desirable, however, there is not enough evidence regarding treatment of CKD-MBD, especially in its early stages, due to its multifactorial pathophysiology and the difficulty in generating adequate animal models. In this study, we evaluated the efficacy of a tissue nonspecific alkaline phosphatase (TNAP) inhibitor, SBI-425 in a CKD-MBD animal model, produced by a combination of nephrectomy and high inorganic phosphate (Pi) diet. This combination induced renal damage, and significantly elevated blood urea nitrogen (BUN). Plasma levels of fibroblast growing factor 23 (FGF-23), parathyroid hormone (PTH) and phosphate were also elevated, leading to ectopic calcification in the kidneys, particularly in the renal tubules. We orally administered SBI-425 twice daily for 12 weeks at doses of 1 and 10 mg/kg, and this treatment significantly inhibited the progression of calcium deposition in the renal tubules. Furthermore, SBI-425 effectively prevented the deterioration of plasma parameters, BUN, FGF-23, PTH, and phosphate. In conclusion, our findings suggest that TNAP inhibition can effectively slow the progression of CKD-MBD by inhibiting the calcification in the renal tubules. These results may have implications for better clinical care of patients with CKD.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144992076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel miniTurbo knock-in mouse reveals a protein interaction network of USP46 in the brain. 一种新型迷你iturbo敲入小鼠揭示了大脑中USP46的蛋白质相互作用网络。

IF 1.2 4区农林科学

Experimental Animals Pub Date : 2025-08-08 DOI: 10.1538/expanim.25-0082

Kazuya Murata, Noa Haneishi, Reiko Nakagawa, Yoko Daitoku, Seiya Mizuno

{"title":"A novel miniTurbo knock-in mouse reveals a protein interaction network of USP46 in the brain.","authors":"Kazuya Murata, Noa Haneishi, Reiko Nakagawa, Yoko Daitoku, Seiya Mizuno","doi":"10.1538/expanim.25-0082","DOIUrl":"https://doi.org/10.1538/expanim.25-0082","url":null,"abstract":"Uncovering protein interaction networks in vivo is essential for understanding physiological and pathological processes. Here, we report the generation of a novel knock-in mouse model expressing miniTurbo, a highly active biotin ligase, fused to the endogenous Usp46 gene. This model enables proximity-dependent biotinylation (BioID) of USP46-associated proteins in the brain. In adult mice, biotinylation was induced by feeding a 0.1% biotin diet. We further evaluated whether the combination of miniTurbo and dietary biotin supplementation is effective for BioID in the developing brain. Biotinylation was successfully induced in embryonic and neonatal brains via maternal biotin intake, demonstrating the transfer of biotin to the offspring through the placenta during pregnancy and through milk during lactation. This strategy enables proximity labeling under physiological conditions without invasive procedures, such as repetitive subcutaneous injections, during developmental stages. Using mass spectrometry, we identified USP46-proximal proteins, including known cofactors WDR48 and WDR20, in the adult brain. Gene Ontology analysis revealed enrichment in postsynaptic pathways, consistent with known localization of USP46. Among the identified proteins, PLPP3, a phospholipid phosphatase, was significantly downregulated in the hippocampus of Usp46-knockout mice. These findings establish the USP46-miniTurbo knock-in mouse as a powerful tool for in vivo interactome analysis and provide new insights into the molecular functions of USP46 in the brain.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144821001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A partial deletion of the Tardbp 3´UTR affects TDP-43 regulation and leads to motor dysfunction in mice. tardbp3´UTR的部分缺失影响TDP-43的调节并导致小鼠运动功能障碍。

IF 2.2 4区农林科学

Experimental Animals Pub Date : 2025-07-19 DOI: 10.1538/expanim.25-0061

Tra Thi Huong Dinh, Chigusa Imura, Mayu Shiokawa, Shinya Ayabe, Atsushi Yoshiki, Haruhisa Inoue, Takanori Amano

{"title":"A partial deletion of the Tardbp 3´UTR affects TDP-43 regulation and leads to motor dysfunction in mice.","authors":"Tra Thi Huong Dinh, Chigusa Imura, Mayu Shiokawa, Shinya Ayabe, Atsushi Yoshiki, Haruhisa Inoue, Takanori Amano","doi":"10.1538/expanim.25-0061","DOIUrl":"https://doi.org/10.1538/expanim.25-0061","url":null,"abstract":"Amyotrophic lateral sclerosis (ALS) is a devastating neurodegenerative disease that causes the selective loss of motor neurons. A histopathological hallmark of ALS is the cytoplasmic aggregation of TDP-43, a ubiquitously expressed RNA-binding protein involved in transcription and splicing regulation. To prevent abnormal accumulation, TDP-43 controls its expression levels through an autoregulatory feedback loop. While most ALS studies have focused on pathogenic variants that impair the protein function of TDP-43, the mechanisms underlying endogenous TDP-43 dysregulation mediated by non-coding elements, including the 3´ untranslated region (3´UTR), remain incompletely understood. In this study, we generated a mouse model carrying a targeted deletion of the Tardbp 3´UTR that encompasses the TDP-binding region, polyadenylation signals, and alternative intronic sequences. Our findings demonstrate that the Tardbp 3´UTR is essential for normal mouse development. Loss of this region led to decreased Tardbp mRNA expression and embryonic lethality after gastrulation. Young heterozygous mice were phenotypically normal with no overt disruption in TDP-43 autoregulation. However, aged heterozygous mice displayed mild locomotor dysfunction accompanied by a modest increase in spinal cord TDP-43 protein levels and a reduction in motor neuron numbers. These findings indicate that regulatory elements within the Tardbp 3´UTR play a pivotal role in normal development and contribute to TDP-43 pathology relevant to ALS.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144682271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Suppression of USP2 in mouse skeletal muscle: a model of oxidative stress in muscle tissue. 小鼠骨骼肌中USP2的抑制：肌肉组织氧化应激的模型。

IF 2.2 4区农林科学

Experimental Animals Pub Date : 2025-07-19 DOI: 10.1538/expanim.25-0032

Masaki Fujimoto, Tomohito Iwasaki, Marina Hosotani Saito, Naoki Takahashi, Mayuko Hashimoto, Eiki Takahashi, Hiroshi Kitamura

{"title":"Suppression of USP2 in mouse skeletal muscle: a model of oxidative stress in muscle tissue.","authors":"Masaki Fujimoto, Tomohito Iwasaki, Marina Hosotani Saito, Naoki Takahashi, Mayuko Hashimoto, Eiki Takahashi, Hiroshi Kitamura","doi":"10.1538/expanim.25-0032","DOIUrl":"https://doi.org/10.1538/expanim.25-0032","url":null,"abstract":"Emerging evidence indicates that oxidative stress in skeletal muscle is a prerequisite for sarcopenia in diabetic patients. In this study, we show that ubiquitin-specific protease (USP) 2 mitigates the accumulation of reactive oxygen species (ROS) in mature muscle cells. Treatment with ML364, a canonical USP2 inhibitor, robustly increased mitochondrial ROS in mouse C2C12 myotubes and caused an accompanying increase in the glutathione disulfide (GSSG)/glutathione (GSH) ratio. ML364 also caused mitochondrial damage in C2C12 myotubes, resulting in a reduction in intracellular adenosine triphosphate levels. Correspondingly, under diabetic condition, the muscle-specific Usp2-knockout (msUsp2KO) C57BL/6N mice exhibited a significantly higher lipid peroxide level and GSSG/GSH ratio in skeletal muscle than the control mice. The msUsp2KO mice also exhibited augmented insulin resistance and glucose intolerance, but showed no obvious deterioration in muscle weight or histology relative to the control mice. However, damaged mitochondria in the soleus muscle were more frequently observed in msUsp2KO mice than in the control mice. Together, these data suggest that USP2 mitigates ROS accumulation and subsequent mitochondrial damage in muscle cells in mice.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144682272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the role of zolpidem in alleviating cognitive and motor impairments in chronic cerebral hypoperfusion: a rat model study with in vivo and in silico insights. 探索唑吡坦在缓解慢性脑灌注不足的认知和运动障碍中的作用：一项具有体内和计算机见解的大鼠模型研究。

IF 2.2 4区农林科学

Experimental Animals Pub Date : 2025-07-17 DOI: 10.1538/expanim.25-0038

Sherilyn M T Choo, Fatin H Mohamad, Syarifah Maisarah Sayed Mohamad, Jafri Malin Abdullah, Khairul Bariyyah Abd Halim, Azzmer Azzar Abdul Hamid, Ahmad Tarmizi Che Has

{"title":"Exploring the role of zolpidem in alleviating cognitive and motor impairments in chronic cerebral hypoperfusion: a rat model study with in vivo and in silico insights.","authors":"Sherilyn M T Choo, Fatin H Mohamad, Syarifah Maisarah Sayed Mohamad, Jafri Malin Abdullah, Khairul Bariyyah Abd Halim, Azzmer Azzar Abdul Hamid, Ahmad Tarmizi Che Has","doi":"10.1538/expanim.25-0038","DOIUrl":"https://doi.org/10.1538/expanim.25-0038","url":null,"abstract":"The ε-containing GABA (A) receptors (GABAARs), a lesser-studied subtype within the GABAAR family, have garnered attention due to their distinct pharmacological properties and potential involvement in brain injury. Zolpidem (ZPM), a widely used Z-drug, is known to induce paradoxical effects in patients with brain injury, although the underlying molecular mechanisms remain unclear. In this study, a chronic cerebral hypoperfusion (CCH) rat model was established using Permanent Bilateral Occlusion of the Common Carotid Arteries (PBOCCA), followed by administration of ZPM at doses of 1.0, 2.0, and 4.0 mg/kg. Behavioral assessments demonstrated that the 1.0 mg/kg dose of ZPM significantly improved spatial learning and memory acquisition (P<0.01) and enhanced memory retention (P<0.001), whereas higher doses resulted in sedation and cognitive impairment. Immunohistochemical analysis revealed an upregulation of the ε subunit expression in the hippocampal CA1 and CA3 regions of CCH rats (P<0.05), suggesting alterations in receptor composition in response to cerebral hypoperfusion. Further investigation of ZPM's interaction with ε-containing GABAARs (specifically the α1β2ε subtype) was conducted using in silico techniques. Molecular docking identified the α1+/ε- binding interface as a favorable ZPM binding site, with key residues being either conserved or suitably replaced. Molecular dynamics simulations demonstrated that ZPM stabilizes the receptor while permitting conformational flexibility, consistent with its role as a positive allosteric modulator. These findings provide evidence that ZPM interacts with ε-containing GABAARs, potentially explaining its paradoxical effects observed in brain injury models.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144648925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diverse Cre recombinase expression pattern in Albumin-Cre driver rats. 白蛋白驱动大鼠不同Cre重组酶表达模式。

IF 2.2 4区农林科学

Experimental Animals Pub Date : 2025-07-11 Epub Date: 2025-01-22 DOI: 10.1538/expanim.24-0174

Saeko Ishida, Keiko Taguchi, Ryuya Iida, Kosuke Hattori, Hiroaki Taketsuru, Kazuto Yoshimi, Masayuki Yamamoto, Tomoji Mashimo

{"title":"Diverse Cre recombinase expression pattern in Albumin-Cre driver rats.","authors":"Saeko Ishida, Keiko Taguchi, Ryuya Iida, Kosuke Hattori, Hiroaki Taketsuru, Kazuto Yoshimi, Masayuki Yamamoto, Tomoji Mashimo","doi":"10.1538/expanim.24-0174","DOIUrl":"10.1538/expanim.24-0174","url":null,"abstract":"Rats (Rattus norvegicus) have been widely utilized as model animals due to their physiological characteristics, making them suitable for surgical and long-term studies. They have played a crucial role in biomedical research, complementing studies conducted in mice. The advent of genome editing technologies has facilitated the generation of genetically modified rat strains, advancing studies in experimental animals. Among these innovations, Cre-driver rat models have emerged as powerful tools for spatiotemporal control of gene expression. However, their development and characterization remain less advanced compared to mouse models. In this study, we developed liver-targeting Cre knock-in rats and reporter knock-in rats to evaluate Cre recombinase expression profiles in different genetic contexts. Our results revealed that insertion orientation and promoter origin significantly influence Cre expression patterns. Notably, forward insertion of the Albumin (Alb) promoter-driven Cre sequence at the ROSA26 locus resulted in ubiquitous Cre expression, while reverse insertion confined Cre expression predominantly to the liver. Interestingly, Cre expression under an endogenous Alb promoter unexpectedly induced expression in non-liver tissues, which may suggest a potential link to the in vivo dynamics of albumin. These findings underscore the importance of rigorous characterization in Cre-based transgenic systems. By elucidating the roles of promoter origin, insertion site, and orientation, our study provides valuable insights for optimizing Cre-driver rat models. These findings pave the way for refining genetic strategies to enhance tissue specificity and reliability in functional genomics and disease modeling.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"328-334"},"PeriodicalIF":2.2,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12270594/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143022672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0