Experimental Animals最新文献

Comparison of the characteristics of non-alcoholic fatty liver disease in Lepr^em1hwl and Lepr^db/db mice. Leprem1hwl与Leprdb/db小鼠非酒精性脂肪性肝病特征比较

IF 2.2 4区农林科学

Experimental Animals Pub Date : 2025-05-15 DOI: 10.1538/expanim.24-0100

Seungwoo Lee, Jae-Hong Min, Myoung Jun Kim, Somi Yun, Min Kyoung Seo, Jong Kwon Lee

{"title":"Comparison of the characteristics of non-alcoholic fatty liver disease in Leprem1hwl and Leprdb/db mice.","authors":"Seungwoo Lee, Jae-Hong Min, Myoung Jun Kim, Somi Yun, Min Kyoung Seo, Jong Kwon Lee","doi":"10.1538/expanim.24-0100","DOIUrl":"https://doi.org/10.1538/expanim.24-0100","url":null,"abstract":"The Lepr gene encodes a receptor for leptin, a hormone instrumental in the regulation of appetite and metabolism. Mutations in the Lepr gene impair leptin signaling, leading to metabolic dysfunctions and facilitating the development of non-alcoholic fatty liver disease (NAFLD). In this study, we compared the NAFLD-associated phenotypes of two mutant strains of mice, C57BL/6J-Leprem1hwl/Korl (Leprem1hwl) and C57BLKS/J-Leprdb/J (Leprdb/db), carrying different alleles of the Lepr gene. Although both Leprem1hwl and Leprdb/dbmice were characterized by similar obesity phenotypes, leptin resistance, insulin resistance, and glucose intolerance, comparatively, Leprem1hwl mice were found to have relatively more severe hepatic steatosis, along with the upregulated expression of enzymes associated with lipogenesis and triglyceride synthesis, and, notably, the histological characteristics of steatohepatitis were observed only in these mice. In addition, compared with the Leprdb/dbmice, Leprem1hwl mice developed hepatic fibrosis characterized by elevated levels of collagen deposition and expression of profibrotic factors. Moreover, we detected elevated levels of pro-inflammatory mediators and increases in M1 macrophage markers in the serum and liver, respectively, of Leprem1hwl mice. These findings highlight the distinct NAFLD-associated phenotypic differences between Leprem1hwl and Leprdb/dbmice, and thereby indicate that Leprem1hwl mice could serve as a valuable model for studying NAFLD, including steatohepatitis and fibrosis.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144076517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sexual activity is predicted by digit ratio in rats. 大鼠的手指比例可以预测性行为。

IF 2.2 4区农林科学

Experimental Animals Pub Date : 2025-05-14 DOI: 10.1538/expanim.24-0159

Himeka Hayashi, Hirotaka Sakamoto

{"title":"Sexual activity is predicted by digit ratio in rats.","authors":"Himeka Hayashi, Hirotaka Sakamoto","doi":"10.1538/expanim.24-0159","DOIUrl":"https://doi.org/10.1538/expanim.24-0159","url":null,"abstract":"The ratio of the second and fourth digits (2D:4D) is a morphological marker reflecting fetal exposure to sex steroid hormones. This ratio exhibits sexual dimorphism, with males typically showing a significantly lower ratio than females, which results from higher androgen exposure during the fetal period. While studies in humans have suggested a relationship between sexual orientation and the 2D:4D ratio, this relationship in rodents remains elusive. Here, we investigated this relationship using rats as an experimental model. We found that male rats exhibited significantly shorter 2D length than females, resulting in a lower 2D:4D ratio in males, similar to humans. Observations of sexual behavior revealed that males that ejaculated during the first mating test exhibited shorter 2D length compared to males those that did not ejaculate. When males were classified into two groups based on 2D length (long-2D and short-2D groups), short-2D males were more sexually active than long-2D males. Additionally, only short-2D males showed a preference for female odors. These findings suggest that, in rats, 2D length is a useful morphological marker reflecting sexual activity and preference. Furthermore, they provide evidence supporting the potential use of the 2D:4D ratio as a tool for studying the relationship between sexual orientation and the 2D:4D ratio in humans.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144076631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Loss of non-canonical translation initiation factors impairs perinatal cardiac function in mice. 非规范翻译起始因子的缺失损害小鼠围产期心功能。

IF 2.2 4区农林科学

Experimental Animals Pub Date : 2025-05-10 DOI: 10.1538/expanim.25-0021

Takehiro Asai, Ryota Tochinai, Yoshiharu Tsuru, Marie Sekiguchi, Atsushi Minami, Wataru Fujii, Shigeru Kyuwa, Tetsuhiro Ogawa, Shigeru Kakuta

{"title":"Loss of non-canonical translation initiation factors impairs perinatal cardiac function in mice.","authors":"Takehiro Asai, Ryota Tochinai, Yoshiharu Tsuru, Marie Sekiguchi, Atsushi Minami, Wataru Fujii, Shigeru Kyuwa, Tetsuhiro Ogawa, Shigeru Kakuta","doi":"10.1538/expanim.25-0021","DOIUrl":"https://doi.org/10.1538/expanim.25-0021","url":null,"abstract":"Translation regulation is crucial for cellular homeostasis. Recent studies have demonstrated that, in addition to the conventional AUG start codon, eukaryotic mRNA can also possess non-canonical start codons. These non-canonical start codons, including non-AUG codons, can be found both upstream and downstream of the conventional AUG start codon. Translation of these non-canonical open reading frames (ORFs) has been implicated in the development of diseases, such as cardiac diseases, neurodegeneration and cancer development. Non-AUG translation initiation is regulated by eukaryotic initiation factor (eIF) 2A and eIF2D; however, their target non-canonical ORFs, roles in disease development, and the underlying precise mechanisms of translation regulation remain poorly understood. To address these gaps, we generated mice lacking either or both of Eif2a and Eif2d genes on an ICR background and investigated their cardiac function using echocardiography. The results indicated that simultaneous disruption of both Eif2a and Eif2d led to perinatal cardiac impairment, as evidenced by a significant reduction in cardiac contractility as measured by ejection fraction. Furthermore, the absence of phenotypic changes in single knockouts of either Eif2a or Eif2d suggests that eIF2A and eIF2D function redundantly in their molecular roles. These findings underscore the importance of non-AUG translation initiation in maintaining cardiac function and suggest its broader implications in other physiological and pathological processes. We propose the Eif2a and Eif2d double-knockout mice as a novel stress-sensitive animal model to investigate the molecular mechanisms of translation regulation and their contribution to disease pathogenesis.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143974467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Luminescent mouse model of endometriosis: three-dimensional morphology of lesions and cytokine profiles. 子宫内膜异位症小鼠发光模型：病变的三维形态和细胞因子谱。

IF 2.2 4区农林科学

Experimental Animals Pub Date : 2025-05-09 DOI: 10.1538/expanim.25-0044

Nanda Yuli Rahmawati, Tra Thi Huong Dinh, Tomona Oikawa, Akiko Shinogi, Kyoko Ikeda, Masayo Kadota, Masaru Tamura, Takanori Amano, Atsushi Yoshiki

{"title":"Luminescent mouse model of endometriosis: three-dimensional morphology of lesions and cytokine profiles.","authors":"Nanda Yuli Rahmawati, Tra Thi Huong Dinh, Tomona Oikawa, Akiko Shinogi, Kyoko Ikeda, Masayo Kadota, Masaru Tamura, Takanori Amano, Atsushi Yoshiki","doi":"10.1538/expanim.25-0044","DOIUrl":"https://doi.org/10.1538/expanim.25-0044","url":null,"abstract":"The pathophysiology of endometriosis remains incompletely understood, necessitating the development of effective animal models for research. We generated and characterized a luminescent endometriosis mouse model utilizing luminescent B6-CAG-ELuc transgenic mice as uterine tissue donors and B6.Cg-c/c-hr/hr mice as recipients, enabling non-invasive in vivo imaging. Following transplantation of minced uterine tissue fragments into the peritoneal cavity of recipients, we monitored lesion growth via in vivo imaging system on 0, 14, 28, 42 days post transplantation. Morphology of the lesion was observed by dissecting microscopy, X-ray micro-computed tomography, and conventional histology. Inflammation-related serum cytokines were quantified using multiplex immunobeads assay. The growth of endometriotic lesions was efficiently observed by bioluminescence from day 0 through 42 days post transplantation. Comprehensive morphological observations revealed typical endometriotic lesions consisted of multiple fluid-filled cysts lined with single-layered epithelium, associated with glandular epithelial tissues and interstitial stroma. The level of IL-1β, IL-2, IL-6, IL-10, IL-12p70, IFN-γ, and TNF-α was quantified simultaneously in each serum sample to evaluate the temporal changes of each cytokine, showing four distinct patterns: IFN-γ and TNF-α showed continuous increase, IL-12p70 and IL-1β demonstrated gradual increase followed by marked elevation, IL-6 and IL-2 exhibited dramatic increase in later stages, while IL-10 showed transient increase followed by gradual decrease. In conclusion, this luminescent endometriosis mouse model using B6 luminescent transgenic mice as uterine tissue donor and B6.Cg-c/c-hr/hr recipient could be used to investigate comprehensive cytokine profiling in the development of endometriosis.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144005130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Soyasaponin I alleviates inflammation and oxidative stress in chronic obstructive pulmonary disease through inhibiting the MAPK signaling pathway. 大豆皂苷I通过抑制MAPK信号通路减轻慢性阻塞性肺疾病的炎症和氧化应激。

IF 2.2 4区农林科学

Experimental Animals Pub Date : 2025-05-08 DOI: 10.1538/expanim.25-0007

Ruoqi Zhang, Jiabo Yuan, Congyao Wang, Ruiqi Zhao, Fengli Gao, Zhuying Li

{"title":"Soyasaponin I alleviates inflammation and oxidative stress in chronic obstructive pulmonary disease through inhibiting the MAPK signaling pathway.","authors":"Ruoqi Zhang, Jiabo Yuan, Congyao Wang, Ruiqi Zhao, Fengli Gao, Zhuying Li","doi":"10.1538/expanim.25-0007","DOIUrl":"https://doi.org/10.1538/expanim.25-0007","url":null,"abstract":"Chronic obstructive pulmonary disease (COPD) is a prevalent lung disease that mainly induced by cigarette smoking (CS). Soyasaponin I is an amphiphilic oleanane triterpenoid glycoside extracted form Astragali Radix. In order to investigate treatment strategies of COPD, this study focused on the effect of soyasaponin I on the lung tissue of COPD model. The mouse model of COPD was induced by CS exposure for 12 weeks, and was administrated with different doses of soyasaponin I. Subsequently, the morphology and histopathology of lung tissue, the proportion of inflammatory cell, the levels of inflammatory cytokines, and indicators of oxidative stress were assessed and analyzed. The signaling pathway potentially regulated by soyasaponin I in the pathogenesis of COPD were predicted by network pharmacology analysis and validated by western blot. Our results demonstrated that soyasaponin I mitigated the lung injury and bronchial lesions induced by COPD through reducing the lung coefficient, wall area of the bronchioles and Periodic Acid Schiff (PAS)-positive cells in the lung tissue. The CS-induced inflammation and oxidative stress was alleviated by soyasaponin I through reversing the levels of inflammatory cytokines and oxidative stress indicators. In addition, the phosphorylation of p38, JNK and ERK1/2 was activated in COPD model, and was reverted by soyasaponin I in the lung tissue. Collectively, the present study confirmed that soyasaponin I is an effective compound that attenuates the lung injury through inhibiting inflammatory response and oxidative stress via the MAPK signaling pathway.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143994469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of culture-preservation methods to maintain developmental competence in porcine metaphase II (MII) oocytes post-in vitro maturation (IVM). 猪MII卵母细胞体外培养保存方法的优化。

IF 2.2 4区农林科学

Experimental Animals Pub Date : 2025-04-20 Epub Date: 2024-11-29 DOI: 10.1538/expanim.24-0107

Haruhisa Tsuji, Rei Maeyama, Yoko Kato

引用次数: 0

Dual-route administration of balanced anesthesia using medetomidine, midazolam, and butorphanol provides both suitable anesthetic depth and reduced tissue injury in rabbits. 使用美托咪定、咪达唑仑和丁吗啡诺双管齐下进行平衡麻醉，既能为兔子提供合适的麻醉深度，又能减少组织损伤。

IF 2.2 4区农林科学

Experimental Animals Pub Date : 2025-04-20 Epub Date: 2024-11-23 DOI: 10.1538/expanim.24-0132

Risa Iwanaga, Kanako Sumi, Chizuko Kodama, Munekatsu Ita, Mohammad Ibrahim Qasimi, Jun Tamura, Ko Nakanishi, Yasuhiro Yoshida, Masami Morimatsu, Kayoko Matsumura, Teppei Nakamura

{"title":"Dual-route administration of balanced anesthesia using medetomidine, midazolam, and butorphanol provides both suitable anesthetic depth and reduced tissue injury in rabbits.","authors":"Risa Iwanaga, Kanako Sumi, Chizuko Kodama, Munekatsu Ita, Mohammad Ibrahim Qasimi, Jun Tamura, Ko Nakanishi, Yasuhiro Yoshida, Masami Morimatsu, Kayoko Matsumura, Teppei Nakamura","doi":"10.1538/expanim.24-0132","DOIUrl":"10.1538/expanim.24-0132","url":null,"abstract":"Medetomidine, midazolam, and butorphanol (MMB) anesthesia is the preferred choice for rodents but requires excess volume of intramuscular injection in rabbits, which can lead to muscular damage. This study aimed to evaluate a dual-route MMB administration via the intravenous and subcutaneous routes in rabbits. MMB was administered to male Kbs:JW rabbits with an intravenous injection of 0.2 ml/kg followed by a subcutaneous injection of 0.8 ml/kg, totaling 0.2 mg/kg medetomidine, 2.0 mg/kg midazolam, and 2.0 mg/kg butorphanol. We compared the anesthetic effects of this dual-route method with those of intramuscular administration. The dual-route method resulted in a shorter induction time and similar anesthetic duration compared with those of the intramuscular route. While it induced a temporary decrease in body temperature within 30 min post-injection, other vital signs, such as respiration rate, heart rate, and O2 saturation, remained similar. Notably, unlike intramuscular administration, dual-route administration did not increase tissue injury marker levels. This dual-route MMB administration provided sufficient anesthetic depth during surgery, eliminating pain reflexes. Double-dose administration extended anesthetic duration but resulted in rare fatalities, indicating room for protocol improvement. In conclusion, the novel anesthetic method is preferable for injectable anesthesia in rabbits, providing rapid induction and sufficient anesthetic duration, while potentially minimizing muscle injury. This technique may be beneficial for both laboratory and companion animals and significantly enhance animal welfare in anesthesia by reducing the pain associated with injectable anesthesia.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"181-188"},"PeriodicalIF":2.2,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12044349/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142709669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histidine and soy isoflavones co-ingestion induces browning of white adipose tissue and promotes lipolysis in female rats. 组氨酸和大豆异黄酮同时摄入可诱导雌性大鼠白色脂肪组织褐变并促进脂肪分解。

IF 2.2 4区农林科学

Experimental Animals Pub Date : 2025-04-20 Epub Date: 2024-12-13 DOI: 10.1538/expanim.24-0138

Riku Asahi, Haruhide Udagawa, Remiko Oshiro, Shigeru Nakajima, Nobuyuki Kanzawa, Kaori Sano, Yukiko Shimizu, Tadashi Okamura, Takahiko J Fujimi

{"title":"Histidine and soy isoflavones co-ingestion induces browning of white adipose tissue and promotes lipolysis in female rats.","authors":"Riku Asahi, Haruhide Udagawa, Remiko Oshiro, Shigeru Nakajima, Nobuyuki Kanzawa, Kaori Sano, Yukiko Shimizu, Tadashi Okamura, Takahiko J Fujimi","doi":"10.1538/expanim.24-0138","DOIUrl":"10.1538/expanim.24-0138","url":null,"abstract":"Beige adipocytes arise from white adipocytes in response to cold or other stimuli, known as browning of white adipose. Beige adipocytes play a role similar to that of brown adipocytes, express high levels of uncoupling protein 1 (UCP1), and are responsible for energy consumption via heat production, thus aiding in fat loss. Although histidine (His) and soy isoflavones (Iso) co-ingestion reportedly reduces food intake, body weight, and fat accumulation in female rats, the underlying mechanism remains unclear. Therefore, this study aimed to elucidate the mechanisms whereby histidine and soy isoflavones (His-Iso) co-ingestion suppresses fat accumulation. Female rats were fed a control diet or diet containing Iso, His, or His-Iso for 2 weeks, followed by sampling of periovarian white adipose tissue (poWAT) and retroperitoneal white adipose tissue (rWAT) and adipocyte morphology analysis. Additionally, the expression of browning- and lipid metabolism-related genes was examined. Histochemical analysis revealed the presence of multilocular lipid droplets, representative of beige adipocytes, in the poWAT and rWAT of rats in the His-Iso co-ingestion group. Quantitative PCR analysis showed that His-Iso co-ingestion upregulated brown adipocyte and beige adipocyte markers, including UCP1, indicating that His-Iso intake induces beige adipocytes. Moreover, His-Iso co-ingestion upregulated genes related to fatty acid oxidation (carnitine palmitoyl transferase 1A) and lipolysis (adipose triglyceride lipase) in WATs. In conclusion, His-Iso co-ingestion increases UCP1 expression and morphological changes to beige adipocytes, and suppresses fat accumulation by promotion of lipolysis and fatty acid oxidation in WAT.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"239-250"},"PeriodicalIF":2.2,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12044354/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142827816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-frequency ultrasound for assessing the renal characteristics of spontaneous type 2 diabetes mellitus db/db mice. 高频超声评估自发性 2 型糖尿病 db/db 小鼠的肾脏特征。

IF 2.2 4区农林科学

Experimental Animals Pub Date : 2025-04-20 Epub Date: 2024-10-25 DOI: 10.1538/expanim.24-0114

Jiazhi Cao, Hao Feng, Lutong Li, Wenwu Ling, Hong Wang

{"title":"High-frequency ultrasound for assessing the renal characteristics of spontaneous type 2 diabetes mellitus db/db mice.","authors":"Jiazhi Cao, Hao Feng, Lutong Li, Wenwu Ling, Hong Wang","doi":"10.1538/expanim.24-0114","DOIUrl":"10.1538/expanim.24-0114","url":null,"abstract":"There are few ultrasonographic studies on the spontaneous type 2 diabetes mellitus db/db mouse. Our objective was to dynamically investigate and assess renal morphological and hemodynamic changes in spontaneous T2DM db/db mice through high-frequency ultrasound. Eighteen male db/db mice (the model group) and twelve male db/+ mice (the control group) were included. Body weight and fasting blood glucose were measured at the ages of 8, 16 and 32 weeks. High-frequency ultrasound examinations were conducted at the same ages. Compared with those in the control group, hematoxylin-eosin and Masson staining revealed pathological changes in the renal tissue of the db/db mice at 16 weeks of age, and the lesions were significantly aggravated at 32 weeks of age. The body mass of the mice in the model group increased significantly at 8, 16 and 32 weeks of age, and the kidney volume measured by ultrasound also increased with age. Compared with those of the control group, the blood flow scores determined via power Doppler were significantly different. The peak systolic velocity (PSV), end diastolic velocity (EDV), and resistive index (RI) of the renal artery and the PSV, EDV, and RI of the segmental artery were significantly different at the sixteenth week compared with those that at the eighth week. The results of high-frequency ultrasound revealed that the renal hemodynamics of db/db mice changed at the sixteenth weeks.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"151-159"},"PeriodicalIF":2.2,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12044364/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142497650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adriamycin-induced nephropathy models: elucidating CKD pathophysiology and advancing therapeutic strategies. 阿霉素诱导的肾病模型：阐明慢性肾功能衰竭的病理生理学并推进治疗策略。

IF 2.2 4区农林科学

Experimental Animals Pub Date : 2025-04-20 Epub Date: 2024-11-23 DOI: 10.1538/expanim.24-0133

Masaki Watanabe, Hayato R Takimoto, Nobuya Sasaki

{"title":"Adriamycin-induced nephropathy models: elucidating CKD pathophysiology and advancing therapeutic strategies.","authors":"Masaki Watanabe, Hayato R Takimoto, Nobuya Sasaki","doi":"10.1538/expanim.24-0133","DOIUrl":"10.1538/expanim.24-0133","url":null,"abstract":"The Adriamycin-induced nephropathy (AN) model plays a crucial role in advancing our understanding of and research on chronic kidney disease (CKD). This review outlines methodologies for generating AN models in mice and rats, discusses their pathophysiologic and molecular characteristics, highlights their advantages and limitations, describes therapeutic interventions that have been evaluated in these models, and presents future research perspectives. The AN model replicates key features observed in human CKD, such as proteinuria, podocyte injury, glomerulosclerosis, and tubulointerstitial fibrosis. Notably, genetic factors significantly influence the onset and severity of AN, with mutations in the Prkdc gene linked to nephrotoxicity and systemic toxicity. To evaluate therapeutic interventions for CKD, agents such as ACE inhibitors, corticosteroids, and SGLT2 inhibitors have been tested in the AN model, demonstrating promising renoprotective effects. However, the systemic toxicity of Adriamycin and variability across models pose limitations, highlighting the need for caution when translating findings to human CKD. Future advancements in genetic engineering and the application of CRISPR-Cas9 technology are expected to improve the fidelity of AN models to human disease. Additionally, discovery of biomarkers by using the AN model enables us to improve early diagnosis. These efforts are anticipated to deepen our understanding of CKD pathophysiology and contribute to developing more effective diagnostic tools and targeted therapies.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"132-142"},"PeriodicalIF":2.2,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12044353/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142709663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0