Experimental Animals最新文献

{"title":"Electroacupuncture improves cognitive function in high-fat diet/streptozocin-induced type 2 diabetic mice by inhibiting autophagy-related ferroptosis.","authors":"Jingzhi Wang, Zhongyu Huang, Yiwen Li, Qian Li, Xi Li, Li Chen","doi":"10.1538/expanim.24-0072","DOIUrl":"10.1538/expanim.24-0072","url":null,"abstract":"<p><p>At present, there lacks a definitive pharmaceutical intervention or therapeutic approach for diabetes-associated cognitive impairment. Herein, we delved into the impact of electroacupuncture on cognitive function in high-fat diet/streptozocin (HFD/STZ)-induced type 2 diabetes mellitus (T2DM) mice and underlying mechanisms. Hippocampal insulin resistance was determined by western blot analysis. Cognitive function was evaluated by Morris water maze test. The morphology of the hippocampal neurons was observed through hematoxylin & eosin staining and Nissl staining. Synaptic plasticity was assessed by western blot analysis. Immunofluorescence, immunohistochemistry, western blot and real-time PCR were employed to detect the levels of ferroptosis markers, autophagy markers, and netrin-1. Electroacupuncture treatment exhibited ameliorative outcomes on hippocampal insulin resistance, spatial learning, memory function, neuronal damage, and synaptic plasticity in T2DM mice. Furthermore, it effectively suppressed neuronal ferroptosis in the hippocampus by upregulating GPX4 and SLC7A11 expression, and reducing 4-HNE expression. Meanwhile, electroacupuncture intervention increased the levels of Beclin1 and LC3II/LC3I, as well as decreased the levels of p62 and phosphorylated-mTOR in the hippocampus of T2DM mice, suggesting that electroacupuncture facilitated autophagy activation by inhibiting mTOR activity. 3-MA-mediated autophagy inhibition undermined the beneficial effects of electroacupuncture on neuronal ferroptosis and cognitive deficits in T2DM mice. Additionally, the beneficial effects of electroacupuncture on autophagy and ferroptosis was achieved by upregulation of netrin-1 in the hippocampus. Our study revealed that electroacupuncture therapy inhibited neuronal ferroptosis via the activation of autophagy, thereby ameliorating cognitive deficits in T2DM mice.</p>","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"197-208"},"PeriodicalIF":2.2,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12044350/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142794613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-term application of MC903 in mice prolongs the characteristic symptoms of atopic dermatitis, such as inflammation, skin barrier dysfunction, and itching.","authors":"Yuya Hoshino, Kazuyoshi Kirima, Naoya Arichika, Yusuke Kakumoto, Masafumi Shibamori, Satoshi Matsumoto, Hidetaka Hiyama","doi":"10.1538/expanim.24-0088","DOIUrl":"10.1538/expanim.24-0088","url":null,"abstract":"<p><p>Atopic dermatitis (AD) is a chronic skin disease that causes itching and is characterized by recurrent flares and remissions. The interactions among type 2 inflammation, skin barrier dysfunction, and pruritus play important roles in the pathogenesis of AD. AD symptoms persist for a long period; thus, it is desirable to have disease models that reproduce a prolonged AD-like phenotype. Although MC903-induced AD model mice reportedly exhibit type 2 inflammation, skin barrier dysfunction, and pruritus, the effects of long-term application of MC903 on the changes in these symptoms over time are not fully understood. To clarify this point, we conducted a long-term time course analysis of these symptoms by applying MC903 to the ears of mice every other day for four weeks. Increased ear thickness, transepidermal water loss, number of scratching events, and serum IgE levels were observed in the MC903 model. Histological analysis revealed the infiltration of granulocytes and CD3-positive T cells and an increase in mast cells in the dermis. Furthermore, analyses of mRNA and protein expression in ear tissue revealed increased expression of thymic stromal lymphopoietin, IL-4, IL-13, and IL-33, which are involved in type 2 inflammation. All these changes were observed within two weeks after the initial application of MC903 and thereafter persisted throughout the experimental period. In conclusion, our data indicate that the long-term application of MC903 prolongs the duration of the three major symptoms of AD.</p>","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"276-285"},"PeriodicalIF":2.2,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12044355/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142893041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological analysis of autophagy in axonal degeneration in gracile axonal dystrophy mice.","authors":"Yusuke Tokuhara, Shinichiro Ukon, Shohei Watanabe, Yoshiki Tatsumi, Hiroo Yoshikawa, Masaki Ohmuraya, Takashi Kimura","doi":"10.1538/expanim.24-0041","DOIUrl":"10.1538/expanim.24-0041","url":null,"abstract":"<p><p>Gracile axonal dystrophy (gad) mutant mice present with autosomal recessive inherited sensory ataxia in the early stages, followed by age-dependent motor ataxia. This phenotype is caused by a mutation in the ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCH-L1) gene and leads to a lack of expression of UCH-L1 protein, ubiquitin-proteasome which is related to the autophagy pathway and the ubiquitin-proteasome system (UPS). To elucidate the pathophysiology of abnormal protein accumulation in gad mice, we focused on macroautophagy. Using electron microscopy, we detected a double-membrane structure, which was characteristic of autophagosomes, in gad mice. In addition, in immunohistochemistry to investigate the expression levels of autophagy-related proteins in the gracile nuclei of the gad mouse, we found upregulation of LC3 and p62 but not LAMP-2A. These results suggested that a lack of UCH-L1 expression might induce the formation of autophagosomes, but the resulting autophagy flux might be disturbed.</p>","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"173-180"},"PeriodicalIF":2.2,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12044348/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142617015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Canine regional gastric blood flow measurement using perfusion computed tomography.","authors":"Kazuya Kushida, Miu Matsumoto, Mizuki Tamazawa, Kentaro Yamazaki, Eisei Shimizu, Airi Kaneyama, Aritada Yoshimura, Shunsuke Miyahara, Kodai Tashiro, Kija Lee, Masaaki Katayama, Ryuji Fukushima, Miori Kishimoto","doi":"10.1538/expanim.24-0134","DOIUrl":"10.1538/expanim.24-0134","url":null,"abstract":"<p><p>This study aimed to determine the feasibility of using perfusion computed tomography (CT) to assess blood flow in different regions of the stomach in dogs. Dynamic perfusion CT scans were conducted on five beagle dogs, and blood flow analysis was performed using the maximum slope and Patlak plot methods. The findings revealed significant variations in blood flow among the fundus, body, and pylorus of the stomach. Specifically, the body showed approximately 1.3 times higher blood flow than the fundus and approximately 5 times higher blood flow than the pylorus. There were no significant differences in blood flow between the two analysis algorithms. The findings suggest that gastric perfusion CT can accurately detect variations in blood flow within the stomach. Using the maximum slope method for analysis allows for noninvasive and rapid measurement of gastric blood flow. This technique may have clinical applications in detecting submucosal diseases that are challenging to identify with endoscopies and serve as a valuable noninvasive tool for longitudinal observations in experimental animal studies.</p>","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"209-215"},"PeriodicalIF":2.2,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12044351/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142779534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic potential of omentin-1 in preeclampsia: enhancing fetal outcomes, vascular function, and reducing inflammation.","authors":"Min Song, Bo Jiao, Xiu-Juan Tian, Bang-Ruo Qi","doi":"10.1538/expanim.24-0092","DOIUrl":"10.1538/expanim.24-0092","url":null,"abstract":"<p><p>This study evaluated the therapeutic potential of omentin-1 in preeclampsia (PE). A PE-like mouse model received recombinant human omentin-1 protein (rh-omentin) from gestation day (gd) 13.5 to 16.5. On gd 17.5, fetuses and placentas were weighed, and soluble fms-like tyrosine kinase-1 (sFlt-1) levels were measured. Maternal aortic rings were used for ex vivo vascular reactivity assays. Inflammatory factors and Krüppel-like factor 2 (KLF2) expression in placental and aortic tissues were assessed using qPCR. Human umbilical vein endothelial cells (HUVECs) were exposed to plasma from PE patients or healthy pregnant individuals to evaluate omentin-1 and KLF2 expression by qPCR, with additional evaluation of KLF2 after rh-omentin treatment. Rh-omentin treatment reduced blood pressure in the PE-like model, accompanying by increased fetal and placental weights and higher fetal/placental weight ratios compared to untreated PE mice. Additionally, rh-omentin enhanced endothelial function in maternal aortic rings, as well as reduced placental necrosis and promoted CD31-positive vasculature in the labyrinth zone. Moreover, rh-omentin decreased pro-inflammatory factors in aortic and placental tissues of PE mice. KLF2 expression was restored in both aortic and placental tissues of PE mice and in HUVECs exposed to PE plasma following rh-omentin treatment. Rh-omentin improved fetal and placental outcomes in PE-like mice, enhancing vascular function and reducing inflammation in aortic and placental tissues. It also restored KLF2 expression in PE tissues and HUVECs exposed to PE plasma, suggesting therapeutic potential for addressing endothelial dysfunction in PE.</p>","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"216-228"},"PeriodicalIF":2.2,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12044363/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142794614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing the neural differentiation capabilities of genetically asymmetric mouse F1 hybrid embryonic stem cell lines.","authors":"Ayaka Saito, Hidemasa Kato, Hidenori Kiyosawa","doi":"10.1538/expanim.24-0094","DOIUrl":"10.1538/expanim.24-0094","url":null,"abstract":"<p><p>Allele-specific, monoallelic expression in diploid organisms represents an extreme case of allelic imbalance resulting from incompatibility between cis- and trans-elements. Due to haploinsufficiency, such monoallelic expression can lead to sporadic genetic diseases. In mice, allelic imbalances can be introduced into F1 offspring from inbred strains. Previously, we established F1 hybrid embryonic stem (ES) cell lines derived from four different mouse strains, each belonging to a different subspecies with substantial genetic polymorphisms. In this study, we investigated the neural differentiation capacity of the established ES cell lines. By introducing different culture conditions, which kept the ES cells undifferentiated under various pluripotencies, we succeeded in differentiating the majority of ES cell lines (eight out of eleven) with our default neural differentiation paradigm. Still, three lines exhibited insufficient differentiation despite combining culture conditions promoting undifferentiated as well as differentiated status. In addition, Ube3a imprinting was seen in two lines. Our findings contribute to the methodological understanding of mouse ES cell pluripotency and lead to the practical utility of F1 hybrid ES cells as a model for studying phenotypes resulting from gene locus interactions.</p>","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"143-150"},"PeriodicalIF":2.2,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12044357/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142461307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Royal jelly reduced non-rapid eye movement sleep fragmentation and restored sleep stability in diet-induced obese mice.","authors":"Chiaki Sugiura, Mao Sato, Shun Tanaka, Nobuaki Okumura, Akira Terao","doi":"10.1538/expanim.24-0128","DOIUrl":"10.1538/expanim.24-0128","url":null,"abstract":"<p><p>Royal jelly (RJ) is recognized due to its high nutritional value and potential health benefits. Previous research showed that RJ supplementation decreased fat accumulation, resulting in weight loss and improvements in hyperglycemia and insulin resistance in high-fat diet (HFD)-induced obese mice. To expand the weight-reducing properties of RJ, this study aimed to investigate the effects of RJ supplementation on HFD-induced obese mice with impaired sleep stabilization. Over a 20-week period, the C57BL/6J mice were divided into the following dietary groups: normal diet (ND), ND supplemented with 5% lyophilized RJ powder (ND+RJ), HFD, and HFD supplemented with 5% lyophilized RJ powder (HFD+RJ) groups. Compared with the HFD group, the HFD+RJ group exhibited a significant reduction in body weight via a decrease in fat mass. Moreover, much like the ND group, the HFD+RJ group demonstrated improvements in the fragmentation of non-rapid eye movement (NREM) sleep and wakefulness. These processes contributed to the reestablishment of sleep/wake continuity and restored the overall stability of sleep. In contrast, the ND+RJ and ND groups exhibited a similar sleep/wake architecture. Thus, RJ supplementation in the ND demonstrated no substantial effect on sleep/wake. According to these findings, dietary RJ improves the sleep/wake architecture and restores sleep stability. Hence, RJ is a promising dietary component for addressing obesity and restoring sleep stability.</p>","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"300-309"},"PeriodicalIF":2.2,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12044360/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142978095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential regulation of K⁺-Cl^- cotransporter 2 (KCC2) and Na⁺-K⁺-Cl^- cotransporter 1 (NKCC1) expression by zolpidem in CA1 and CA3 hippocampal subregions of the lithium-pilocarpine status epilepticus rat model.","authors":"Muhammad Zulfadhli Othman, Mohd Hamzah Mohd Nasir, Wan Amir Nizam Wan Ahmad, Jafri Malin Abdullah, Ahmad Tarmizi Che Has","doi":"10.1538/expanim.24-0120","DOIUrl":"10.1538/expanim.24-0120","url":null,"abstract":"<p><p>Status epilepticus is linked to cognitive decline due to damage to the hippocampus, a key structure involved in cognition. The hippocampus's high vulnerability to epilepsy-related damage is the main reason for this impairment. Convulsive seizures, such as those observed in status epilepticus, can cause various hippocampal pathologies, including inflammation, abnormal neurogenesis, and neuronal death. Interestingly, substantial evidence points to the therapeutic potential of the sedative/hypnotic agent zolpidem for neurorehabilitation in brain injury patients, following the unexpected discovery of its paradoxical awakening effect. In this study, we successfully established an ideal lithium-pilocarpine rat model of status epilepticus, which displayed significant deficits in hippocampal-dependent learning and memory. The Morris water maze test was used to assess zolpidem's potential to improve learning and memory, as well as its impact on anxiety-like behavior and motor function. Immunohistochemical staining and fluorescence analysis were used to examine the effect of zolpidem on K⁺-Cl^- cotransporter 2 (KCC2) and Na⁺-K⁺-Cl^- cotransporter 1 (NKCC1) protein expression in the hippocampal CA1 and CA3. Our findings showed that zolpidem did not improve learning and memory in status epilepticus rats. Additionally, its sedative/hypnotic effects were not apparent in the status epilepticus condition. However, immunohistochemical results revealed that zolpidem significantly restored altered NKCC1 levels in the CA1 and CA3 to levels similar to those seen in normal rats. These findings suggest that zolpidem may contribute to molecular restoration, particularly through its impact on NKCC1 protein expression in the hippocampus, which is crucial for proper inhibitory neurotransmission in the brain.</p>","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"286-299"},"PeriodicalIF":2.2,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12044352/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142947083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biological characteristics of age-related changes in C57BL/6 mice sub-strains in the national center for geriatrics and gerontology aging farm.","authors":"Noboru Ogiso, Julio A Almunia, Yoshiko Munesue, Shunsuke Yuri, Akihiko Nishikimi, Atsushi Watanabe, Morihiro Inui, Kazumichi Takano, Shumpei Niida","doi":"10.1538/expanim.24-0095","DOIUrl":"10.1538/expanim.24-0095","url":null,"abstract":"<p><p>Aging is a complex biological process. Several animal models, including nematodes, Drosophila, and rodents, have been used in research on aging mechanisms and the extension of healthy life expectancy. The present study investigated the physiological and anatomical changes associated with aging in two sub-strains of aged C57BL/6 mice used in aging research: C57BL/6NCrSlc (B6N) and C57BL/6J (B6J). The survival rate before 24 months old (mo) was higher in B6J mice than in B6N mice; however, after 24 mo, it was markedly lower in the former than in the latter. Body weight increased in male C57BL/6 mice until 15-18 mo and in females until 21-24 mo and then began to decrease. Body temperature was lower in B6N mice than in B6J mice until 24 mo. Food and water intakes increased from 18 mo in both strains. The incidence of alopecia was higher in female C57BL/6J mice from 3 mo. Necropsy findings showed a high rate of spontaneous tumors in both sub-strains. The incidence of cutaneous ulcerative infections and hepatic pathologies was significantly higher in the B6N strain. A high incidence of renal lesions was also observed in B6J mice, particularly in males. These results provide insights into the characteristics of these sub-strains and the phenotypic changes associated with aging, which will facilitate the use of aged mice as a quality resource for geriatric and gerontological research.</p>","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"229-238"},"PeriodicalIF":2.2,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12044361/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142812316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomics and metabolomics analysis of the pathogenesis of a novel hyperlipidemia-susceptible rat strain.","authors":"Xiufeng Ai, Qian Zhang, Quanxin Ma, Mingsun Fang, Keyan Zhu, Yueqin Cai, Qinqin Yang, Lizong Zhang","doi":"10.1538/expanim.24-0080","DOIUrl":"10.1538/expanim.24-0080","url":null,"abstract":"<p><p>Wistar-SD Hypercholesterolemia (WSHc) Rat is a novel hyperlipidemia-susceptible rat that we discovered and bred earlier, which can be used as an ideal animal model for the study of non-alcoholic fatty liver disease (NAFLD). However, its pathogenesis of hyperlipidemia and genetic and biological characteristics need to be further investigated. In the current study, WSHc rats were fed a high-fat diet (HFD) and standard chow (SC), with age-matched Wistar rats as the control group undergoing the same treatment, followed by serum lipid level measurement. It was found that HFD-fed WSHc rats developed dyslipidemia. Transcriptomic analysis was performed to detect genes associated with cholesterol metabolism in the liver, and 119 differentially expressed genes were discovered through bioinformatics analysis and molecular biology verification. Additionally, Srebf1 was identified as a HUB gene and Nr1d1 as an independent key gene using the protein-protein interaction network and one-cluster clustering analysis. The two genes had also been further validated in molecular biology experiments and were consistent with transcriptomic results. Serum lipid metabolomics analysis identified 7 lipid subclasses and 84 lipid molecules using UHPLC-Q-TOF/MS. There were 62 and 70 lipid molecules with significant differences in the metabolic profiles of serum lipid mediators in the WSHc+HFD group compared to the WSHc+SC and Wistar+HFD groups, respectively, and the differential metabolites were mainly produced via sphingolipid and glycerophospholipid metabolism. In sum, the hypercholesterolemia model can be established with WSHc rats after the HFD induction, and the pathogenesis involves the Srebf1 and Nr1d1 genes and the sphingolipid and glycerophospholipid metabolism pathways.</p>","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"160-172"},"PeriodicalIF":2.2,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12044359/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142575520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}