Experimental Animals最新文献_第5页

Dominant effect of a single amino acid mutation in the motor domain of myosin VI on hearing in mice. 肌球蛋白VI运动结构域单个氨基酸突变对小鼠听力的显性影响。

IF 2.2 4区农林科学

Experimental Animals Pub Date : 2025-04-20 Epub Date: 2024-12-17 DOI: 10.1538/expanim.24-0141

Yuta Seki, Shumpei P Yasuda, Xuehan Hou, Kayoko Tahara, Ornjira Prakhongcheep, Ai Takahashi, Yuki Miyasaka, Hirohide Takebayashi, Yoshiaki Kikkawa

{"title":"Dominant effect of a single amino acid mutation in the motor domain of myosin VI on hearing in mice.","authors":"Yuta Seki, Shumpei P Yasuda, Xuehan Hou, Kayoko Tahara, Ornjira Prakhongcheep, Ai Takahashi, Yuki Miyasaka, Hirohide Takebayashi, Yoshiaki Kikkawa","doi":"10.1538/expanim.24-0141","DOIUrl":"10.1538/expanim.24-0141","url":null,"abstract":"An unconventional myosin, myosin VI gene (MYO6), contributes to recessive and dominant hearing loss in humans and mice. The Kumamoto shaker/waltzer (ksv) mouse is a model of deafness resulting from a splice-site mutation in Myo6. While ksv/ksv homozygous mice are deaf due to cochlear hair cell stereocilia fusion at the neonatal stage, the hearing phenotypes of ksv/+ heterozygous mice have been less clear. Due to this splicing error, most MYO6 protein expression is lost in ksv mice; however, MYO6 with a single amino acid mutation (p.E461K) remains expressed. In this study, we investigated the hearing phenotypes and effect of a p.E461K mutation in ksv/+ heterozygous mice. Hearing tests indicated that hearing loss in ksv/+ mice arises concurrently at both low and high frequencies. In the low-frequency region, stereocilia fusions were detected in the inner hair cells of ksv/+ mice. Expression analysis revealed abnormal MYO6 expression and localization, along with atypical expression of proteins in the basal region of the stereocilia, suggesting that these abnormalities may contribute to stereocilia fusion in ksv/+ mice. Conversely, although the expression patterns of MYO6 and stereociliary basal-region proteins appeared normal in the cochlear area corresponding to high-frequency sounds, stereocilia loss in the outer hair cells was observed in ksv/+ mice. These findings suggest that the ksv/+ mice exhibit distinct mechanisms underlying hearing loss across areas responsible for low- and high-frequency hearing, differing from those previously reported in heterozygous Myo6 mice with loss-of-function and missense mutant alleles.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"251-263"},"PeriodicalIF":2.2,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12044356/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142853162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DNA repair protein RAD50 is involved in the streptozotocin-induced diabetes susceptibility of mice. DNA修复蛋白RAD50参与链脲佐菌素诱导的小鼠糖尿病易感性。

IF 2.2 4区农林科学

Experimental Animals Pub Date : 2025-04-20 Epub Date: 2024-12-24 DOI: 10.1538/expanim.24-0071

Yuki Miyasaka, Tomoki Maegawa, Takuma Nagura, Misato Kobayashi, Naru Babaya, Hiroshi Ikegami, Fumihiko Horio, Tamio Ohno

{"title":"DNA repair protein RAD50 is involved in the streptozotocin-induced diabetes susceptibility of mice.","authors":"Yuki Miyasaka, Tomoki Maegawa, Takuma Nagura, Misato Kobayashi, Naru Babaya, Hiroshi Ikegami, Fumihiko Horio, Tamio Ohno","doi":"10.1538/expanim.24-0071","DOIUrl":"10.1538/expanim.24-0071","url":null,"abstract":"Streptozotocin (STZ) is widely used as a pancreatic beta-cell toxin to induce experimental diabetes in rodents. Strain-dependent variations in STZ-induced diabetes susceptibility have been reported in mice. Differences in STZ-induced diabetes susceptibility are putatively related to pancreatic beta-cell fragility via DNA damage response. In this study, we identified two STZ-induced diabetes susceptibility regions in chromosome 11 (Chr11) of Nagoya-Shibata-Yasuda (NSY) mice via congenic mapping using the C3H-11NSY consomic strains, in which the entire Chr11 of STZ-resistant C3H/He (C3H) mice was replaced with that of NSY mice, and named them STZ susceptibility region for NSY (Ssnsy)-1 and -2, respectively. Screening for variants in the Ssnsy1 region revealed that NSY mice exhibited a characteristic missense c.599G>T (p.G200V) variant in a highly conserved region within the DNA repair gene, RAD50 double-strand break repair protein (Rad50). Subsequently, we generated R2B1-Rad50 knock-in mice, in which c.599T in Rad50 of STZ-susceptible C3H.NSY-R2B1 subcongenic mice was replaced with c.599G via genome editing. Compared with C3H.NSY-R2B1 mice, and R2B1-Rad50 knock-in mice showed suppressed hyperglycemia, incidence of diabetes, and decrease in plasma insulin levels following single high-dose and multiple low-dose injections of STZ. Our results suggest Rad50 as a susceptibility gene for STZ-induced diabetes that is involved in pancreatic beta-cell fragility. Forward genetic approaches using inbred mouse strains with STZ susceptibility as a phenotypic indicator will further elucidate the molecular mechanisms of pancreatic beta-cell destruction via DNA damage response.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"264-275"},"PeriodicalIF":2.2,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12044362/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142893387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acot1 overexpression alleviates heart failure by inhibiting oxidative stress and cardiomyocyte apoptosis through the Keap1-Nrf2 pathway. Acot1过表达通过Keap1-Nrf2途径抑制氧化应激和心肌细胞凋亡，从而缓解心力衰竭。

IF 2.2 4区农林科学

Experimental Animals Pub Date : 2025-04-16 DOI: 10.1538/expanim.24-0129

Xiaolu Hou, Guoling Hu, Heling Wang, Ying Yang, Qi Sun, Xiuping Bai

{"title":"Acot1 overexpression alleviates heart failure by inhibiting oxidative stress and cardiomyocyte apoptosis through the Keap1-Nrf2 pathway.","authors":"Xiaolu Hou, Guoling Hu, Heling Wang, Ying Yang, Qi Sun, Xiuping Bai","doi":"10.1538/expanim.24-0129","DOIUrl":"https://doi.org/10.1538/expanim.24-0129","url":null,"abstract":"Heart failure (HF) is a clinical syndrome related to multiple causes, including oxidative stress. Acyl-CoA thioesterase 1 (Acot1) is an enzyme in fatty acids metabolism, but it remains unclear in HF. Transverse aortic coarctation induced HF mouse model and hypoxia-stimulated cardiomyocyte (HL-1) model were established. Acot1 expression was down-regulated in heart tissues of HF mice. AAV9-mediated Acot1 overexpression improved cardiac function and pathological injury of heart tissues in TAC-induced HF mice. Acot1 overexpression ameliorated oxidative stress in heart tissues of HF mice and hypoxia-stimulated HL-1 cells, as indicated by reduced ROS and MDA levels and elevated SOD and GSH levels. We found that Acot1 overexpression inhibited apoptosis both in vivo and in vitro, with decreased protein levels of cleaved PARP, cleaved caspase-3, and cleaved caspase-9. Mechanically, Acot1 activated Keap1-Nrf2 pathway, leading to the nuclear translocation of Nrf2 and increased Nrf2-regulated gene NQO1 expression. Rescue experiment indicated that ML385 (Nrf2 inhibitor) abolished the effect of Acot1 overexpression on oxidative stress. Collectively, these results suggested that Acot1 overexpression protects heart from injury by inhibiting oxidative stress and apoptosis, possibly through activating Keap1-Nrf2 pathway.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143995907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual-route medetomidine-alfaxalone-butorphanol anesthesia: a refined protocol for balanced anesthesia in male rabbits. 美托咪定-阿尔法沙龙-布托啡诺双路麻醉：一种完善的雄性家兔平衡麻醉方案。

IF 2.2 4区农林科学

Experimental Animals Pub Date : 2025-04-15 DOI: 10.1538/expanim.24-0151

Risa Iwanaga, Munekatsu Ita, Kanako Sumi, Chizuko Kodama, Mohammad Ibrahim Qasimi, Jun Tamura, Ko Nakanishi, Kayoko Matsumura, Masami Morimatsu, Yasuhiro Yoshida, Teppei Nakamura

{"title":"Dual-route medetomidine-alfaxalone-butorphanol anesthesia: a refined protocol for balanced anesthesia in male rabbits.","authors":"Risa Iwanaga, Munekatsu Ita, Kanako Sumi, Chizuko Kodama, Mohammad Ibrahim Qasimi, Jun Tamura, Ko Nakanishi, Kayoko Matsumura, Masami Morimatsu, Yasuhiro Yoshida, Teppei Nakamura","doi":"10.1538/expanim.24-0151","DOIUrl":"https://doi.org/10.1538/expanim.24-0151","url":null,"abstract":"Injectable anesthesia is widely used in laboratory animals because of its ease of administration and minimal equipment requirements. However, it necessitates careful monitoring as well as thermal and oxygen support. This study evaluated the efficacy of medetomidine-alfaxalone-butorphanol (MAB) anesthesia in male rabbits using a dual-route administration protocol. The anesthetic doses were as follows: medetomidine, 0.2 mg/kg; alfaxalone, 2.0 mg/kg; and butorphanol, 2.0 mg/kg. MAB anesthesia, administered via intravenous and subcutaneous routes, induced rapid and smooth induction, achieving anesthetic scores comparable to those of medetomidine-midazolam-butorphanol (MMB) anesthesia. MAB anesthesia resulted in mild hypothermia during the procedure. Upon atipamezole administration, rabbits under MAB anesthesia exhibited faster recovery of the righting reflex and respiration rate than those under MMB. Importantly, no abnormal behaviors, such as jumping or agitation, were observed during induction or recovery, as reported with alfaxalone use in other species. Both protocols maintained spontaneous breathing, although transient hypoxemia was observed in all rabbits. The dual-route MAB protocol provided effective anesthesia while addressing the limitations of conventional MMB anesthesia in rabbits, suggesting its potential as a refined anesthetic method for this species. Unlike mice, which showed weaker anesthetic effects with MAB compared to MMB, MAB demonstrated superior anesthetic properties in rabbits. This study highlights the importance of species-specific anesthetic protocols and the potential benefits of MAB anesthesia in rabbits, particularly its smooth induction and recovery profile, without adverse behaviors often associated with alfaxalone in other species.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143974465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Disruption of intestinal barrier and dysbiosis of gut microbiota in an experimental rhesus macaque model with 6-year diabetes mellitus. 6年糖尿病恒河猴模型的肠道屏障破坏和肠道菌群失调。

IF 2.2 4区农林科学

Experimental Animals Pub Date : 2025-01-17 DOI: 10.1538/expanim.24-0125

Xiangyu Fu, Xiang Ren, Maoyuan Zhao, Lan Li, Yaojia Zhou, Yanrong Lu, Chengshi Wang

{"title":"Disruption of intestinal barrier and dysbiosis of gut microbiota in an experimental rhesus macaque model with 6-year diabetes mellitus.","authors":"Xiangyu Fu, Xiang Ren, Maoyuan Zhao, Lan Li, Yaojia Zhou, Yanrong Lu, Chengshi Wang","doi":"10.1538/expanim.24-0125","DOIUrl":"https://doi.org/10.1538/expanim.24-0125","url":null,"abstract":"This study aims to clarify the disruption of gut barrier and dysbiosis of the microbiota in an experimental macaque model with 6-year diabetes mellitus (DM), and provide evidence for the application of therapeutic strategies targeting the human microbiota in the future. A single intravenous injection of high-dose streptozotocin was used to induce the type 1 diabetes (T1D) macaque model. Hematoxylin-Eosin (HE) and Periodic Acid Schiff (PAS) staining were conducted to observe colon morphological changes. The composition of gut microbiota was detected using 16S rRNA gene sequencing, and bioinformatics analysis was adopted to predict alterations in the microbial phenotype and function. Obvious intestinal inflammation and decreased goblet cells were observed in T1D macaques. 16S rRNA gene sequencing suggested a significantly different β diversity of the microbiota in the T1D group, where expanded Proteobacteria (dominantly Escherichia-Shigella) and Actinomycetota (formerly known as Actinobacteria) replaced the dominance of Bacillota (formerly known as Firmicutes) and Bacteroidota (formerly known as Bacteroidetes), indicating an imbalance in the microbial composition. Archaea was identified as a biomarker between groups. Moreover, with the reduction of beneficial bacteria (Lactobacillaceae) and the increase of pro-inflammatory bacteria and opportunistic pathogens (Enterobacteriaceae), the phenotypes of the microbiota were reversed, resulting in abnormal up- (e.g., carbohydrate and amino acid metabolism) or down-regulation (e.g., protein digestion and absorption) of multiple metabolic pathways. There were intestinal structural disorders and gut microbiota dysbiosis in T1D macaques, indicating that strategies targeting gut microbiota may be effective to treat metabolic diseases like DM.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143002798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mouse NOD/Shi and NSY/Hos strains infected with Plasmodium berghei ANKA are models for experimental cerebral malaria. 小鼠 NOD/Shi 和 NSY/Hos 株感染了伯格氏疟原虫 ANKA，是实验性脑疟疾的模型。

IF 2.2 4区农林科学

Experimental Animals Pub Date : 2025-01-10 Epub Date: 2024-07-26 DOI: 10.1538/expanim.24-0023

Tamio Ohno, Nozomi Iwatake, Yuki Miyasaka

{"title":"Mouse NOD/Shi and NSY/Hos strains infected with Plasmodium berghei ANKA are models for experimental cerebral malaria.","authors":"Tamio Ohno, Nozomi Iwatake, Yuki Miyasaka","doi":"10.1538/expanim.24-0023","DOIUrl":"10.1538/expanim.24-0023","url":null,"abstract":"In humans, cerebral malaria is the most common cause of malaria-related mortality. Mouse C57BL/6 (B6) sub-strains are the major model system for experimental cerebral malaria (ECM) as they show similar pathophysiology to human cerebral malaria after infection with the rodent malaria parasite Plasmodium berghei ANKA. This model system has been used to analyze the molecular mechanisms of cerebral malaria. To develop new mouse models, we analyzed the ECM susceptibility of NOD/Shi (NOD) and NSY/Hos (NSY) strains established from the non-inbred ICR strain. Both NOD and NSY strains exhibited clinical symptoms and pathologies similar to ECM in C57BL/6J (B6J) mice and died within 11 days of infection. Thus, the NOD and NSY strains are susceptible to ECM and may be useful as new ECM models. The ECM susceptibility of both strains is suggested to be due to homozygosity for the cerebral malaria susceptibility allele of the ECM susceptible ICR strain. Although analyses using B6 sub-strains have proposed that complement component 5 (C5) plays an important role in ECM pathogenesis, we found that C5 was not essential as the ECM susceptible NOD strain is C5 deficient. Thus, results obtained from B6 sub-strains may not reflect the full picture of ECM in mice. Comparative analyses of multiple ECM models will contribute to a more accurate identification of the factors essential for ECM.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"31-38"},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11742482/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141787662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dosage and organic acid residue of myelin oligodendrocyte glycoprotein_35-55 peptide influences immunopathology and development of Bacillus Calmette-Guérin induced experimental autoimmune encephalomyelitis. MOG35-55肽的剂量和有机酸残留对卡介苗诱导的实验性自身免疫性脑脊髓炎（EAE）的免疫病理和发展的影响

IF 2.2 4区农林科学

Experimental Animals Pub Date : 2025-01-10 Epub Date: 2024-07-10 DOI: 10.1538/expanim.24-0012

Xiaoyan Han, Ying Wang, Kehua Zhang, Tao Na, Tingting Wu, Xiaofang Hao, Yuxuan Jin, Yuchun Wang, Haohan Wang, Shufang Meng

{"title":"Dosage and organic acid residue of myelin oligodendrocyte glycoprotein35-55 peptide influences immunopathology and development of Bacillus Calmette-Guérin induced experimental autoimmune encephalomyelitis.","authors":"Xiaoyan Han, Ying Wang, Kehua Zhang, Tao Na, Tingting Wu, Xiaofang Hao, Yuxuan Jin, Yuchun Wang, Haohan Wang, Shufang Meng","doi":"10.1538/expanim.24-0012","DOIUrl":"10.1538/expanim.24-0012","url":null,"abstract":"Experimental autoimmune encephalomyelitis (EAE) serves as a model for studying multiple sclerosis, with immunization strategies utilizing myelin oligodendrocyte glycoprotein (MOG)35-55 peptide, emulsified in adjuvant enriched with Mycobacterium tuberculosis (Mtb). This study examined the effects of Bacillus Calmette-Guérin (BCG) as an adjuvant, alongside the impact of MOG35-55 peptide doses and their residual counter ions on EAE development. We found that BCG can be effectively used to induce EAE with similar incidence and severity as heat-killed H37Ra, contingent upon the appropriate MOG35-55 peptide dose. Different immunization doses of MOG35-55 peptide significantly affect EAE development, with higher doses leading to a paradoxical reduction in disease activity, probably due to peripheral tolerance mechanisms. Furthermore, doses of MOG35-55 peptides with acetate showed a more pronounced effect on disease development compared to those containing trifluoroacetic acid (TFA), suggesting the potential influence of residual counter ions on EAE activity. We highlighted the feasibility of applying BCG to the establishment of EAE for the first time. Our findings emphasized the importance of MOG35-55 peptide dosage and composition in modulating EAE development, offering insights into the mechanisms of autoimmunity and tolerance. This could have implications for autoimmune disease research and the design of therapeutic strategies.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"16-30"},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11742474/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141579361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of inhaled anesthesia for Octodon degus using electroencephalography. 利用脑电图优化八爪鱼的吸入麻醉。

IF 2.2 4区农林科学

Experimental Animals Pub Date : 2025-01-10 Epub Date: 2024-08-22 DOI: 10.1538/expanim.24-0017

Yuki Ikai, Goro A Nagura-Kato, Shinsuke H Sakamoto, Akio Shinohara, Chihiro Koshimoto

{"title":"Optimization of inhaled anesthesia for Octodon degus using electroencephalography.","authors":"Yuki Ikai, Goro A Nagura-Kato, Shinsuke H Sakamoto, Akio Shinohara, Chihiro Koshimoto","doi":"10.1538/expanim.24-0017","DOIUrl":"10.1538/expanim.24-0017","url":null,"abstract":"Physiological responses to inhaled anesthetics vary among species. Therefore, a precise anesthetic technique is important for each individual species. In this study, we focused on the degu (Octodon degus), a small herbivorous rodent. Degus have recently begun to be used as laboratory models for brain research because of certain human-like characteristics, such as spontaneous development of Alzheimer's disease. In this study, we evaluated appropriate induction and maintenance anesthesia conditions for isoflurane and sevoflurane in degus by a stimulation test, electroencephalography (EEG), minimum alveolar concentration (MAC), and vital signs. During induction, more rapid time to loss of the righting reflex and deeper anesthesia in degus were observed in isoflurane. The MAC value for degus were 1.75 ± 0.0% in isoflurane and 2.25 ± 0.27% in sevoflurane. Whereas some degus were awake during maintenance anesthesia using both anesthetics at concentrations of ≤2%, no rats were awake when using sevoflurane at a concentration of 2%. The duration of the total flat EEG, a measure of the depth of maintenance anesthesia, was longer for isoflurane than for sevoflurane. Furthermore, higher concentrations of both anesthetics suppressed the respiratory rate in degus. These new findings regarding inhalation anesthesia in degus will contribute to future developments in the fields of laboratory animals and veterinary medicine.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"93-103"},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11742480/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142016861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hematological and biochemical characterization of aging farm male rat strains in the national center for geriatrics and gerontology. 国家老年医学和老年学中心的老龄化农场雄性大鼠品系的血液学和生物化学特征。

IF 2.2 4区农林科学

Experimental Animals Pub Date : 2025-01-10 Epub Date: 2024-08-08 DOI: 10.1538/expanim.24-0028

Julio A Almunia, Yoshiko Munesue, Haruka Kawasaki, Kazumichi Takano, Chisato Kayahara, Satoko Noma, Nobuko Morikawa, Shumpei Niida, Noboru Ogiso

{"title":"Hematological and biochemical characterization of aging farm male rat strains in the national center for geriatrics and gerontology.","authors":"Julio A Almunia, Yoshiko Munesue, Haruka Kawasaki, Kazumichi Takano, Chisato Kayahara, Satoko Noma, Nobuko Morikawa, Shumpei Niida, Noboru Ogiso","doi":"10.1538/expanim.24-0028","DOIUrl":"10.1538/expanim.24-0028","url":null,"abstract":"Laboratory rats, like mice, are a type of animal commonly used in scientific investigations as well as in basic aging and geriatric research. The selection of a rat strain is an important first step in the planning and design of an experiment due to physiological, anatomical, and ethological variations in each strain, which may significantly modify the expected results. In the present study, we characterized age-related changes, from 3 months old (mo) to 24 mo, in three male rat strains commonly used in medical research: RccHan®️:WIST (RccHan:WIST), F344/NSlc (F344), and Slc:SD Rat (SD). The body weight, water/food consumption, and survival rate of each strain were physiologically evaluated. Hematological and biochemical values were analyzed every three months. Hematological results showed a decrease in lymphocytes and increases in other leukocytes from 12 mo in F344 and SD rats. The incidence of hematological disorder was 10-15% in F344 and SD rats from 18 mo. Increases in hepatic biochemical parameters (alanine transaminase (GPT/ALT) and aspartate transaminase (GOT/AST)) and cytopathological parameters (creatine phosphokinase (CPK)) were observed in male F344 rats at 12 mo. Triglycerides (TG) serum levels were significantly elevated in the 12 mo RccHan:WIST rats, while Lipase (LIP) levels were significantly reduced in 24 mo. The present results revealed significant variations in hematological and biochemical values in the different laboratory male rat strains due to genetic and nutritional-metabolic factors specific to each strain.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"66-82"},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11742475/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141901411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibition of tumor necrosis factor receptor-associated factor 6 alleviates secondary brain injury by reducing neuronal pyroptosis after intracerebral hemorrhage. 抑制 TRAF6 可减少脑出血后神经元的脓毒症，从而减轻继发性脑损伤。

IF 2.2 4区农林科学

Experimental Animals Pub Date : 2025-01-10 Epub Date: 2024-08-07 DOI: 10.1538/expanim.24-0078

Qianxin Hu, Haixin Zeng, Chengao Feng, Wei Tian, Yuxin He, Bing Li

{"title":"Inhibition of tumor necrosis factor receptor-associated factor 6 alleviates secondary brain injury by reducing neuronal pyroptosis after intracerebral hemorrhage.","authors":"Qianxin Hu, Haixin Zeng, Chengao Feng, Wei Tian, Yuxin He, Bing Li","doi":"10.1538/expanim.24-0078","DOIUrl":"10.1538/expanim.24-0078","url":null,"abstract":"Secondary brain injury (SBI) is one of the main causes of high mortality and disability rates following intracerebral hemorrhage (ICH). Tumor necrosis factor receptor-associated factor 6 (TRAF6) plays a crucial role in the process of pyroptosis, and modulating its expression may present a novel therapeutic strategy for mitigating brain injury. This study aims to explore the mechanisms of TRAF6 in pyroptosis after ICH. C57BL/6J mice were used to establish the ICH model. Brain was collected at different time points for q-PCR and western blot to detect the level of TRAF6. After the C25-140 (the TRAF6 inhibitor) was administrated, the mice were divided into four groups. Then, the neurological deficit, brain water content, and blood-brain barrier (BBB) damage were detected. Immunofluorescence and western blot were used to detect the level of pyroptosis proteins, and ELISA and q-PCR were used to detect the levels of IL-18 and IL-1β. TRAF6 expression was upregulated after ICH and was mainly expressed in neurons. Inhibition of TRAF6 expression with C25-140 alleviated neurological deficits and reduced brain edema after ICH. In addition, inhibition of TRAF6 also reduced the expression of pyroptosis inflammasomes such as GSDMD, NLRP3, and ASC, as well as neurological damage caused by IL-18 and IL-1β after ICH. TRAF6 regulates neuronal pyroptosis in SBI after ICH. Inhibition of TRAF6 may be a potential target for alleviating inflammatory damage after ICH.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"39-48"},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11742478/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141901412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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