Experimental Animals最新文献_第6页

PacBio long-read amplicon sequencing of dog leukocyte antigen genes at full-length level in beagle dogs. 比格犬白细胞抗原基因全长水平的PacBio长读扩增子测序。

IF 2 4区农林科学

Experimental Animals Pub Date : 2026-04-22 Epub Date: 2026-01-14 DOI: 10.1538/expanim.25-0111

Hiroya Konno, Jiro Miyamae, Rei Kajitani, Kazuto Kugou, Hiroko Kataoka, Makoto Akai, Tomomichi Ishizaka, Katsuyoshi Chiba

{"title":"PacBio long-read amplicon sequencing of dog leukocyte antigen genes at full-length level in beagle dogs.","authors":"Hiroya Konno, Jiro Miyamae, Rei Kajitani, Kazuto Kugou, Hiroko Kataoka, Makoto Akai, Tomomichi Ishizaka, Katsuyoshi Chiba","doi":"10.1538/expanim.25-0111","DOIUrl":"10.1538/expanim.25-0111","url":null,"abstract":"The major histocompatibility complex (MHC) plays a critical role in individual immune responses and susceptibility to various conditions, including autoimmune diseases and drug reactions. In dogs, the canine MHC (dog leukocyte antigen, DLA) polymorphism is key to understanding immune mechanisms, but technical challenges have impeded its comprehensive genetic analysis. This study addressed these issues by using a novel DLA genotyping method combining long-range PCR and PacBio single-molecule real-time sequencing to analyze the full-length DLA class I and II gene sequences in 83 beagle dogs from two different strains (TOYO and Marshall), which are commonly used as laboratory animals. As a result of genotyping using the full-length sequences, 9, 5, 2, 6, and 8 extended alleles were newly discovered for the DLA class I genes in DLA-88, DLA-12, DLA-88L, DLA-64, and DLA-79, respectively. For the DLA class II genes, 11, 18, 12, and 8 extended alleles were newly discovered in DLA-DRA, DLA-DRB1, DLA-DQA1, and DLA-DQB1, respectively. There were 25 haplotypes consisting of extended alleles, in contrast to only 10 haplotypes classified using only peptide binding site sequences. Furthermore, comparisons between the strains revealed differences in haplotype frequencies and genetic differentiation. The full-length analysis also provided preliminary insights into regulatory elements, such as promoter and CpG island polymorphisms in DLA-DQB1. The results of this research have important implications for the understanding of the relationship between DLA polymorphism at full length and individual immune responses in dogs.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"250-261"},"PeriodicalIF":2.0,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13100700/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145984880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrative network pharmacology and experimental study of Qingda granule in hypertension-induced endothelial dysfunction. 清大颗粒治疗高血压内皮功能障碍的综合网络药理学及实验研究。

IF 2 4区农林科学

Experimental Animals Pub Date : 2026-04-22 Epub Date: 2025-10-30 DOI: 10.1538/expanim.25-0080

Yanyan Yang, Qiurong Xie, Jingyi Zeng, Meizhu Wu, Daxin Chen, Wenqiang Zhang, Chenyu Lai, Aling Shen, Dawei Lian, Jun Peng

{"title":"Integrative network pharmacology and experimental study of Qingda granule in hypertension-induced endothelial dysfunction.","authors":"Yanyan Yang, Qiurong Xie, Jingyi Zeng, Meizhu Wu, Daxin Chen, Wenqiang Zhang, Chenyu Lai, Aling Shen, Dawei Lian, Jun Peng","doi":"10.1538/expanim.25-0080","DOIUrl":"10.1538/expanim.25-0080","url":null,"abstract":"Endothelial dysfunction (ED) plays a pivotal role in the pathogenesis of hypertension and its associated vascular complications. Qingda granule (QDG) exhibits significant antihypertensive properties and demonstrates therapeutic potential in ameliorating vascular dysfunction. This study aimed to explore QDG's role in alleviating endothelial injury in hypertension. An L-NAME (Nω-Nitro-L-arginine methyl ester)-induced hypertensive mouse model was used to evaluate the effects of QDG on blood pressure and endothelial function. Endothelial function was assessed through histological analysis, nitric oxide (NO) quantification, and vascular response measurements. To explore underlying mechanisms, network pharmacology was conducted using databases such as HERB, SwissTargetPrediction and STRING. Key pathways related to inflammation and cell adhesion were identified. Based on these findings, immunohistochemical staining was conducted to analyze the expression of phosphorylation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) p65 (p-NF-κB p65), NF-κB p65, intercellular adhesion molecule-1 (ICAM-1), and tumor necrosis factor-α (TNF-α) in vascular tissues. QDG treatment significantly reduced blood pressure, increased NO levels, and enhanced endothelial nitric oxide synthase (eNOS) expression in L-NAME-induced hypertensive mice, indicating its potential to restore endothelial function. Experimental validation further confirmed that QDG markedly suppressed the expression of p-NF-κB p65, TNF-α, and ICAM-1 in vascular tissues. These results suggest that QDG alleviates hypertension-induced ED primarily by inhibiting inflammation and endothelial adhesion via the NF-κB signaling pathway. Overall, QDG presents a promising therapeutic candidate for managing hypertension and its vascular complications.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"131-143"},"PeriodicalIF":2.0,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13100706/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145400285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Body weight loss without reduction in food consumption observed in cynomolgus monkeys during non-clinical toxicity studies. 在非临床毒性研究中观察到食蟹猴体重减轻而不减少食物消耗。

IF 2 4区农林科学

Experimental Animals Pub Date : 2026-04-22 Epub Date: 2025-10-25 DOI: 10.1538/expanim.25-0047

Kazuaki Takahashi, Norio Hike, Hiroyuki Ogura, Takayuki Okamura, Dai Yamamoto, Junko Sato

{"title":"Body weight loss without reduction in food consumption observed in cynomolgus monkeys during non-clinical toxicity studies.","authors":"Kazuaki Takahashi, Norio Hike, Hiroyuki Ogura, Takayuki Okamura, Dai Yamamoto, Junko Sato","doi":"10.1538/expanim.25-0047","DOIUrl":"10.1538/expanim.25-0047","url":null,"abstract":"In pharmaceutical development, weight loss is occasionally observed in monkeys during non-clinical toxicity studies and can be difficult to differentiate from drug effects. This study retrospectively analyzed data from control group monkeys without drug treatment to investigate the incidence of weight loss and its physiological and pathological characteristics. We also investigated potential improvements through enhanced animal welfare. In the 4- and 13-week toxicity studies conducted at the test facility from 2010 to 2022, 684 control group monkeys were investigated. Among them, 3 animals in the 4-week toxicity studies and 5 animals in the 13-week toxicity studies showed a weight change rate of less than -10%, resulting in an incidence rate of 1.2%. However, these animals had adequate food consumption. Animals in the 4-week toxicity studies showed signs of stress in histopathology. Additionally, 2/3 animals in the 4-week toxicity studies had decreased blood glucose levels and 1/5 animal in the 13-week toxicity study fell into a crouching posture, suggesting hypoglycemia that was alleviated with glucose administration, indicating stress-induced metabolic abnormalities. From 2015, an enrichment program was implemented to improve animal welfare. Prior to this program, 2.4% of animals showed a weight change rate of less than -10%, which dropped to 0.25% post-implementation, suggesting the program's effectiveness in reducing stress. These results clarify the characteristics of animals that lose weight during toxicity studies and suggest that improving animal welfare can reduce the incidence rate.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"121-130"},"PeriodicalIF":2.0,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13100701/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145372217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hematologic and biochemical changes associated with age and strain in aged B6 mice breeding in the national center of geriatrics and gerontology. 在国家老年医学和老年学中心饲养的老年B6小鼠中，血液学和生化变化与年龄和品系相关。

IF 2 4区农林科学

Experimental Animals Pub Date : 2026-04-22 Epub Date: 2025-11-05 DOI: 10.1538/expanim.25-0071

Julio A Almunia, Yoshiko Munesue, Noboru Ogiso, Shunsuke Yuri, Haruka Kawasaki, Nobuko Morikawa, Satoko Noma, Kazumichi Takano, Atsushi Watanabe, Shumpei Niida, Akihiko Nishikimi

{"title":"Hematologic and biochemical changes associated with age and strain in aged B6 mice breeding in the national center of geriatrics and gerontology.","authors":"Julio A Almunia, Yoshiko Munesue, Noboru Ogiso, Shunsuke Yuri, Haruka Kawasaki, Nobuko Morikawa, Satoko Noma, Kazumichi Takano, Atsushi Watanabe, Shumpei Niida, Akihiko Nishikimi","doi":"10.1538/expanim.25-0071","DOIUrl":"10.1538/expanim.25-0071","url":null,"abstract":"At the National Center of Geriatric and Gerontology (NCGG), aged mice and rats are used in research on aging and the treatment and prevention of gerontological diseases. Some of the most commonly used mouse strains in our center and general research were the C57BL/6J (B6J) and C57BL/6N (B6N). In this study, hematological and biochemical changes related to age, strain, and sex, from 3 months (mo) to 24 mo, were characterized every 3 mo in the B6J and B6N strains. Hematological results showed that in B6J males at 24 mo, the levels of WBC, especially lymphocytes, were higher than in the B6N strain. In males B6J, the number of CD4+ T cells did not decrease significantly between 6 and 24 mo, but in females and strain B6N, the number of CD4+ T cells decreased significantly. The levels of red blood cells (RBC) and hemoglobin (HGB) were reduced with age in all strains, while the number of platelets (PLT) increased. Biochemical parameters, Blood urea nitrogen (BUN) and Creatinine (CRE) in B6J males were significantly higher than in the other groups at 24 mo. Glutamate oxalacetate transaminase/aspartate aminotransferase (GOT/AST) and glutamate pyruvate transaminase/alanine aminotransferase (GPT/ALT) levels were higher in the B6N strain than the B6J strain at 24 mo. The present results revealed significant variations in hematological and biochemical parameters between the two strains and between sexes as a result of genetic and hormonal differences in laboratory mice.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"172-193"},"PeriodicalIF":2.0,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13100696/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145451400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stingless bee propolis promotes hair follicle regeneration and melanocyte function in chemotherapy-induced alopecia mouse model. 无刺蜂胶促进化疗性脱发小鼠模型毛囊再生和黑素细胞功能。

IF 2 4区农林科学

Experimental Animals Pub Date : 2026-04-22 Epub Date: 2025-11-05 DOI: 10.1538/expanim.25-0060

Jonna Rose C Maniwang, Yulan Tang, Mark Joseph M Desamero, Chen Wang, Wataru Fujii, Dunfu Eer, Shigeru Kyuwa, James K Chambers, Kazuyuki Uchida, Yuri Kominami, Hideki Ushio, Cleofas R Cervancia, Maria Amelita C Estacio, Shigeru Kakuta

{"title":"Stingless bee propolis promotes hair follicle regeneration and melanocyte function in chemotherapy-induced alopecia mouse model.","authors":"Jonna Rose C Maniwang, Yulan Tang, Mark Joseph M Desamero, Chen Wang, Wataru Fujii, Dunfu Eer, Shigeru Kyuwa, James K Chambers, Kazuyuki Uchida, Yuri Kominami, Hideki Ushio, Cleofas R Cervancia, Maria Amelita C Estacio, Shigeru Kakuta","doi":"10.1538/expanim.25-0060","DOIUrl":"10.1538/expanim.25-0060","url":null,"abstract":"Chemotherapy-induced alopecia (CIA) is one of the most apparent symptoms of side effects in a cancer patient undergoing chemotherapy using anti-cancer drugs, resulting in distress and a lower quality of life. Hence, this study investigated the protective and regenerative effects of Philippine stingless bee propolis on CIA in a murine model. Female C57BL/6N mice were subjected to hair cycle synchronization through depilation, followed by cyclophosphamide (CYP) administration to induce hair loss and graying. Daily topical application of 99.5% ethanol extracted propolis diluted twice with water was performed for 30 days. Results revealed that propolis-treated mice exhibited increased folliculogenesis and epidermal thickness, but not hair length, and improved melanogenesis compared to controls. Immunohistochemical and gene expression analyses revealed increased Ki67+ proliferative cells and reduced apoptosis (TUNEL+ cells) at the early 48 h of topical treatment. Moreover, propolis upregulated expressions of Lef1 and melanogenic genes (Tyr, Tyrp1, Dct) at 30 days of treatment. These findings suggest that Philippine stingless bee propolis promotes hair follicle regeneration and melanocyte function, offering a potential natural therapeutic approach for CIA.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"156-171"},"PeriodicalIF":2.0,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13100695/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145451388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A nonsense mutation in the Mocos gene induces xanthinuria, obstructive nephropathy, and anemia in rats. Mocos基因的无义突变可引起大鼠黄尿症、阻塞性肾病和贫血。

IF 2 4区农林科学

Experimental Animals Pub Date : 2026-04-22 Epub Date: 2025-12-03 DOI: 10.1538/expanim.25-0127

Mao Urasaki, Kana Nagasaka, Minori Kido, Kenta Hayashi, Ayumi Watanabe, Kosuke Hattori, Takahiro Sekiguchi, Mitsuru Kuwamura, Miyuu Tanaka, Tomoji Mashimo, Takashi Kuramoto

{"title":"A nonsense mutation in the Mocos gene induces xanthinuria, obstructive nephropathy, and anemia in rats.","authors":"Mao Urasaki, Kana Nagasaka, Minori Kido, Kenta Hayashi, Ayumi Watanabe, Kosuke Hattori, Takahiro Sekiguchi, Mitsuru Kuwamura, Miyuu Tanaka, Tomoji Mashimo, Takashi Kuramoto","doi":"10.1538/expanim.25-0127","DOIUrl":"10.1538/expanim.25-0127","url":null,"abstract":"Xanthinuria type II is a rare hereditary disorder caused by mutations in the MOCOS gene, leading to dual deficiency of xanthine dehydrogenase and aldehyde oxidase. To establish a robust animal model for this condition, we generated Mocos knock-in (KI) rats carrying the Arg419Ter nonsense mutation identified in Japanese patients. Homozygous KI rats exhibited severe growth retardation, anemia, and reduced survival, with all individuals dying by 14 weeks of age. Biochemical analyses revealed elevated levels of hypoxanthine and xanthine, along with decreased uric acid in both serum and urine, confirming xanthinuria. Homozygous KI rats also showed increased blood creatinine (CRE) and urea nitrogen (UN), and decreased urinary CRE and UN, indicating renal dysfunction. Histopathological examination showed obstructive nephropathy characterized by tubular atrophy, crystal deposition, and inflammation. Compared to existing mouse models, Mocos KI rats demonstrated extended lifespan, enabling more detailed investigation of disease mechanisms. This rat model provides a valuable tool for studying the pathogenesis of xanthinuria type II and exploring potential therapeutic strategies.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"202-213"},"PeriodicalIF":2.0,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13100697/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145667851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional behavioral profiling of multiple Cre-driver rat lines. 多种cred驱动大鼠系的功能行为分析。

IF 2 4区农林科学

Experimental Animals Pub Date : 2026-04-22 Epub Date: 2026-01-09 DOI: 10.1538/expanim.25-0115

Asahi Haijima, Hirohiko Imai, Natsuki Matsushita, Kohtaro Morita, Kazuto Kobayashi, Saeko Ishida, Ryuya Iida, Tomoji Mashimo, Masahide Asano

{"title":"Functional behavioral profiling of multiple Cre-driver rat lines.","authors":"Asahi Haijima, Hirohiko Imai, Natsuki Matsushita, Kohtaro Morita, Kazuto Kobayashi, Saeko Ishida, Ryuya Iida, Tomoji Mashimo, Masahide Asano","doi":"10.1538/expanim.25-0115","DOIUrl":"10.1538/expanim.25-0115","url":null,"abstract":"The Cre-loxP system has been widely used in neuroscience for spatial and temporal control of gene expression. Although it is considered a powerful genetic tool, increasing evidence suggests that the Cre recombinase itself, without targeting loxP sites, may affect brain function and behavior. In this study, we assessed behavioral phenotypes in several Cre-driver rat lines, each expressing Cre under a different promoter (CAG, Pvalb, TH, Drd2, Tac1, or Thy1) to determine whether Cre expression influences behavioral outcomes. Behavioral testing included open field (for locomotor activity), hot plate (for nociceptive responses), prepulse inhibition (for sensorimotor gating), and contextual and cued fear conditioning (for associative learning). The Drd2-Cre rats exhibited significantly increased locomotor activity and movement speed compared to wild-type controls, suggesting heightened baseline activity. The CAG-Cre rats spent more time in the center area of the open field and showed reduced freezing during fear conditioning, suggesting changes in emotional regulation or learning. In contrast, no significant differences in the nociceptive responses or prepulse inhibition were observed in any of the Cre-driver lines. Following behavioral testing, we conducted structural MRI scans and found no obvious abnormalities in brain morphology across any of the Cre-driver lines, suggesting that the observed behavioral changes may reflect subtle neural alterations. These results suggested that Cre expression can produce promoter-dependent behavioral alterations, particularly in emotion or cognition. Therefore, behavioral validation of Cre-driver lines is recommended in neural circuit studies.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"241-249"},"PeriodicalIF":2.0,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13100707/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145959264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exposure to xylene early in life induces memory abnormalities of adult male mice. 幼年接触二甲苯会引起成年雄性小鼠的记忆异常。

IF 2 4区农林科学

Experimental Animals Pub Date : 2026-04-22 Epub Date: 2025-12-17 DOI: 10.1538/expanim.25-0064

Hirokatsu Saito, Kousuke Suga, Koji Fujihara, Motoh Mutsuga, Satoshi Yokota, Takuya Nishimura, Satoshi Kitajima

{"title":"Exposure to xylene early in life induces memory abnormalities of adult male mice.","authors":"Hirokatsu Saito, Kousuke Suga, Koji Fujihara, Motoh Mutsuga, Satoshi Yokota, Takuya Nishimura, Satoshi Kitajima","doi":"10.1538/expanim.25-0064","DOIUrl":"10.1538/expanim.25-0064","url":null,"abstract":"Xylene, an aromatic hydrocarbon, is widely used as a solvent in industries, such as printing and rubber and leather manufacturing. Despite the significant industrial benefits of xylene, there are increasing concerns about its environmental and health impacts. However, the effects of early exposure to xylene on the central nervous system are poorly understood. Here, using novel and unique approach, we investigated the neurobehavioral effects of xylene using postnatal lactation C57BL/6N male mice (Mus musculus) exposed to 0 (control) or 2 different doses (2 or 20 ppm) of xylene for 7 consecutive days at 22 h/d. The concentrations of xylene used corresponded respectively to 40 and 400 times the indoor air quality standard in Japan. We examined the effects of xylene on the murine central nervous system using a battery of behavioral tests, comprising the open field test, light-dark transition test, and contextual-cued fear conditioning test at 12 weeks of age. We found that mice exposed to xylene (2 and 20 ppm) presented reduced spatial-associative or tone-cued associative memory in the contextual-cued fear conditioning test. In addition, immunohistochemical analysis revealed a decrease in doublecortin positive cells in the hippocampal dentate gyrus in the xylene exposure group compared with the control group. We confirmed that xylene exposure during postnatal lactation affects the formation of the neural circuit base and behavior in adulthood, suggesting that it is detrimental to postnatal brain development.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"222-233"},"PeriodicalIF":2.0,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13100703/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145774140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neu5Ac promotes high-fat diet-induced progression of atherosclerosis in ApoE-deficient mice. Neu5Ac促进apoe缺陷小鼠高脂肪饮食诱导的动脉粥样硬化进展。

IF 2 4区农林科学

Experimental Animals Pub Date : 2026-04-22 Epub Date: 2025-12-03 DOI: 10.1538/expanim.25-0100

Dong Huang, Chengyong Yin, Di Wang

引用次数: 0

Intron polymorphism in Camk2d is associated with ventricular arrhythmias in normal adult Sprague-Dawley rats. Camk2d内含子多态性与正常成年sd大鼠室性心律失常有关。

IF 2 4区农林科学

Experimental Animals Pub Date : 2026-04-22 Epub Date: 2025-11-01 DOI: 10.1538/expanim.25-0074

Rong Luo, Chunyun Zhao, Yi Wang, Yilin He, Chang Liu, Xiaoping Li, Xin Cao

{"title":"Intron polymorphism in Camk2d is associated with ventricular arrhythmias in normal adult Sprague-Dawley rats.","authors":"Rong Luo, Chunyun Zhao, Yi Wang, Yilin He, Chang Liu, Xiaoping Li, Xin Cao","doi":"10.1538/expanim.25-0074","DOIUrl":"10.1538/expanim.25-0074","url":null,"abstract":"Calcium/calmodulin-dependent protein kinase II (CAMKII) is a critical regulator of cardiac electrophysiology. However, the role of the four bases deletion polymorphism in Camk2d which codes delta subunit of CAMKII, particularly those involving intron sequences, remains poorly understood. This study aimed to investigate the impact of Camk2d c.1044+125_128delGTTT missing polymorphism on cardiac morphology and arrhythmogenesis in normal adult Sprague-Dawley (SD) rats. A total of 85 SD rats were genotyped by Sanger sequencing, revealing a distribution of 25.9% wild-type (WT), 48.2% heterozygous, and 25.9% homozygous variants. Echocardiography, Hematoxylin-Eosin staining, Masson's trichrome staining and transmission electron microscopy indicated no significant differences in cardiac structure or baseline function among the three groups. In freely moving rats, premature atrial arrhythmias were detected in 2 of 9 WT rats, 1 of 9 heterozygous rats, and 1 of 9 homozygous rats. Premature ventricular contractions (PVCs) were observed in none of 9 WT or homozygous rats, 3 of 9 heterozygous rats, with one heterozygous rat exhibiting frequent PVCs. Electrical programmed stimulation revealed a higher incidence of inducible atrial fibrillation in homozygous rats compared to WT rats and a higher incidence of inducible ventricular tachycardia in heterozygous rats compared to WT rats. These findings suggest that deletion polymorphism in the intron sequences of Camk2d are unexpectedly common in normal SD rat populations and that such polymorphism predispose to ventricular arrhythmias without overt structural heart disease. Our study highlights the potential arrhythmogenic risk associated with non-coding DNA sequence alterations in Camk2d and underscores the importance of genetic screening in experimental animal models.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"144-155"},"PeriodicalIF":2.0,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13100694/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145437669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0