Experimental Animals最新文献_第7页

Dual specificity protein phosphatase 5, transcriptionally inhibited by SRY-box transcription factor 11, inhibits T helper 2 differentiation in CD4⁺ T cells: a promising therapeutic target for allergic rhinitis. 被SOX11转录抑制的Dusp5抑制CD4+ T细胞中Th2的分化：变应性鼻炎的一个有希望的治疗靶点

IF 2 4区农林科学

Experimental Animals Pub Date : 2026-04-22 Epub Date: 2025-10-25 DOI: 10.1538/expanim.25-0076

Li Jiang, Chunrui Wang, Wei Han, Shijia Xu, Qi Hu

{"title":"Dual specificity protein phosphatase 5, transcriptionally inhibited by SRY-box transcription factor 11, inhibits T helper 2 differentiation in CD4+ T cells: a promising therapeutic target for allergic rhinitis.","authors":"Li Jiang, Chunrui Wang, Wei Han, Shijia Xu, Qi Hu","doi":"10.1538/expanim.25-0076","DOIUrl":"10.1538/expanim.25-0076","url":null,"abstract":"Allergic rhinitis (AR) is an inflammatory disorder driven primarily by aberrant T helper 2 (Th2) differentiation in CD4+ T cells. Although dual-specificity phosphatase 5 (DUSP5) has been implicated in inflammatory and autoimmune regulation, its role in AR remains unexplored. In this study, an AR mouse model was established via intraperitoneal sensitization and intranasal challenge with ovalbumin. We observed significant downregulation of DUSP5 expression in the nasal mucosa, particularly within CD4+ cells. To elucidate its function, a lentiviral vector overexpressing DUSP5 was constructed and used to transduce naive CD4+ T cells isolated from BALB/c mouse spleens. Overexpression of DUSP5 suppressed Th2-specific cytokine production and inhibited Th2 differentiation. Mechanistic investigations using a luciferase reporter assay revealed that Dusp5 is transcriptionally repressed by SRY-box transcription factor 11 (SOX11), a known transcription factor that promotes the progression of AR. Furthermore, DUSP5 overexpression counteracted the pro-Th2 effects mediated by SOX11. These results demonstrate that DUSP5, transcriptionally inhibited by SOX11, attenuates AR-associated inflammation by restraining Th2 differentiation. Our findings identify DUSP5 as a potential therapeutic target for AR.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"110-120"},"PeriodicalIF":2.0,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13100704/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145372191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ring finger protein 10 is atherosclerosis protective and modulates macrophage polarization. RNF10对动脉粥样硬化具有保护作用并调节巨噬细胞极化。

IF 2 4区农林科学

Experimental Animals Pub Date : 2026-04-22 Epub Date: 2025-12-02 DOI: 10.1538/expanim.25-0088

Ke-Xin Zhao, Shu-Xu Jin, Ming-Hao Li

{"title":"Ring finger protein 10 is atherosclerosis protective and modulates macrophage polarization.","authors":"Ke-Xin Zhao, Shu-Xu Jin, Ming-Hao Li","doi":"10.1538/expanim.25-0088","DOIUrl":"10.1538/expanim.25-0088","url":null,"abstract":"Macrophages can develop into pro-inflammatory M1-like macrophages and anti-inflammatory M2-like macrophages when stimulated by distinct internal environment. Dynamic changes of the two kinds of macrophages play key roles in atherosclerosis progression. The study aims to explore the role of ring finger protein 10 (RNF10) in regulating macrophage polarization during atherosclerosis. Mice with macrophage-specific depletion of RNF10 (RNF10Mac-KO/ApoE-/-) and control mice (RNF10fl/fl/ApoE-/-) mice were fed with high-fat diet to generate atherosclerotic lesion, from which peritoneal macrophages were isolated and transfected with RNF10-overexpressing vector. Murine macrophages, RAW264.7, were transfected with RNF10-overexpressing vector or RNF10 siRNA and stimulated with oxidized low-density lipoprotein (ox-LDL) to induce foam cell formation. The RNF10Mac-KO/ApoE-/- mice showed greater atherosclerotic lesions, more resident macrophages, higher expression of iNOS (M1-like macrophage marker), and lower expression of Arginase-1 (M2-like macrophage marker) than the RNF10fl/fl/ApoE-/- mice. RNF10 overexpression could reduce expressions of IL-1β, IL-6, and iNOS (M1 marker genes), increase expressions of IL-10 and Arg-1 (M2 marker genes) in the peritoneal macrophages isolated from RNF10Mac-KO/ApoE-/- mice. RNF10 overexpression reduced lipid accumulation in ox-LDL-induced foam cells, whereas RNF10 silencing yielded opposite results. Our data suggest that RNF10 is associated with M1-like macrophage suppression and M2-like increase, indicating RNF10 in macrophages has an anti-atherosclerotic role.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"214-221"},"PeriodicalIF":2.0,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13100705/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145667819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Young Investigator Award. 青年研究者奖。

IF 2 4区农林科学

Experimental Animals Pub Date : 2026-01-01 DOI: 10.1538/expanim.75suppl-L2

引用次数: 0

Symposium 4. 研讨会4。

IF 2 4区农林科学

Experimental Animals Pub Date : 2026-01-01 DOI: 10.1538/expanim.75suppl-S4

引用次数: 0

Symposium 2. 研讨会2。

IF 2 4区农林科学

Experimental Animals Pub Date : 2026-01-01 DOI: 10.1538/expanim.75suppl-S2

引用次数: 0

A novel miniTurbo knock-in mouse reveals a protein interaction network of USP46 in the brain. 一种新型迷你iturbo敲入小鼠揭示了大脑中USP46的蛋白质相互作用网络。

IF 1.2 4区农林科学

Experimental Animals Pub Date : 2026-01-01 Epub Date: 2025-08-08 DOI: 10.1538/expanim.25-0082

Kazuya Murata, Noa Haneishi, Reiko Nakagawa, Yoko Daitoku, Seiya Mizuno

{"title":"A novel miniTurbo knock-in mouse reveals a protein interaction network of USP46 in the brain.","authors":"Kazuya Murata, Noa Haneishi, Reiko Nakagawa, Yoko Daitoku, Seiya Mizuno","doi":"10.1538/expanim.25-0082","DOIUrl":"10.1538/expanim.25-0082","url":null,"abstract":"Uncovering protein interaction networks in vivo is essential for understanding physiological and pathological processes. Here, we report the generation of a novel knock-in mouse model expressing miniTurbo, a highly active biotin ligase, fused to the endogenous Usp46 gene. This model enables proximity-dependent biotin labeling (BioID) of USP46-associated proteins in the brain. In adult mice, biotinylation was induced by feeding a 0.1% biotin diet. We further evaluated whether the combination of miniTurbo and dietary biotin supplementation is effective for BioID in the developing brain. Biotinylation was successfully induced in embryonic and neonatal brains via maternal biotin intake, demonstrating the transfer of biotin to the offspring through the placenta during pregnancy and through milk during lactation. This strategy enables proximity labeling under physiological conditions without invasive procedures, such as repetitive subcutaneous injections, during developmental stages. Using mass spectrometry, we identified USP46-proximal proteins, including known cofactors WDR48 and WDR20, in the adult brain. Gene Ontology analysis revealed enrichment in postsynaptic pathways, consistent with known localization of USP46. Among the identified proteins, PLPP3, a phospholipid phosphatase, was significantly downregulated in the hippocampus of Usp46-knockout mice. These findings establish the USP46-miniTurbo knock-in mouse as a powerful tool for in vivo interactome analysis and provide new insights into the molecular functions of USP46 in the brain.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"63-72"},"PeriodicalIF":1.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12826572/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144821001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rat polyomavirus 2 infection: secondary publication. 大鼠多瘤病毒2型感染-继发出版物。

IF 1.2 4区农林科学

Experimental Animals Pub Date : 2026-01-01 Epub Date: 2025-09-03 DOI: 10.1538/expanim.25-0072

Miyuu Tanaka

{"title":"Rat polyomavirus 2 infection: secondary publication.","authors":"Miyuu Tanaka","doi":"10.1538/expanim.25-0072","DOIUrl":"10.1538/expanim.25-0072","url":null,"abstract":"In 2016, an outbreak of Rattus norvegicus polyomavirus 2 (RatPyV2) infection was reported in a colony of X-linked severe combined immunodeficiency (XSCID) rats in the United States. While RatPyV2 infection persists asymptomatically in immunocompetent rats, immunodeficient XSCID rats develop variable respiratory symptoms, emaciation, impaired breeding performance, and systemic deteriorating condition. RatPyV2 is an epitheliotropic virus targeting epithelial cells of the salivary glands, Harderian glands, extraorbital lacrimal glands, respiratory system, and reproductive or accessory reproductive organs. Histopathologically, the formation of large basophilic nuclear inclusion bodies in the infected epithelial cells is a characteristic feature, along with hyperplasia or dysplasia. Glandular atrophy and loss, accompanied by fibrosis and mononuclear cell infiltration, are also observed in the salivary glands, Harderian glands, and extraocular lacrimal glands. In particular, the parotid salivary glands are prone to be severely and extensively affected with relatively severe and diffuse lesions even at one month of age. Severely affected animals also develop interstitial pneumonia. Among target tissues, the parotid salivary glands appear to be higher susceptible to RatPyV2, therefore pathological examination and PCR examination of the salivary glands, including the parotid salivary glands, are essential for the diagnosis of RatPyV2 infection. This review paper provides a comprehensive summary of the features (clinical signs, pathological findings, and transmission), diagnostic methods, and prevalence of RatPyV2 infection, based on our research and reports from research groups in the United States.This is a secondary publication of \"Rat polyomavirus 2 infection\" [25] published in JALAS Newsletter (in Japanese) 2021, 70 (1): 17-25. It also includes updated content.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"1-9"},"PeriodicalIF":1.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12826565/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144992006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Educational efficacy of training videos and simulators for teaching basic mouse experimental skills to novice veterinary students. 训练视频及模拟器对兽医学新手小鼠基本实验技能的教学效果。

IF 1.2 4区农林科学

Experimental Animals Pub Date : 2026-01-01 Epub Date: 2025-09-20 DOI: 10.1538/expanim.25-0093

Atsushi Tsukamoto, Thum Su Zan, Makie Nitta, Hiromitsu Yoshida, Hirotaka Katahira, Yoshiharu Fujita, Satoshi Takagi, Shinichiro Nakamura

{"title":"Educational efficacy of training videos and simulators for teaching basic mouse experimental skills to novice veterinary students.","authors":"Atsushi Tsukamoto, Thum Su Zan, Makie Nitta, Hiromitsu Yoshida, Hirotaka Katahira, Yoshiharu Fujita, Satoshi Takagi, Shinichiro Nakamura","doi":"10.1538/expanim.25-0093","DOIUrl":"10.1538/expanim.25-0093","url":null,"abstract":"Alternative educational tools, such as training videos and simulators, are recommended in the education of laboratory animal science. However, evidence supporting their educational utility in the training of rodent experimental techniques remains limited. In this study, we assessed the utility of alternative educational tools in the practice of laboratory animal science for novice veterinary students. 149 students participated in a stepwise program beginning with lectures, followed by preparatory learning sessions using training videos and two types of mouse simulators (a silicone-based model and fabric toy mouse), and then hands-on training with live mice. The program covered basic techniques: habituation, restraint, and vaginal smear sampling for estrous cycle determination. A survey-based evaluation was conducted to assess the educational utility of alternative educational tools. The contribution of each preparatory resource (videos, lectures, simulators, printed materials, and notes) to skill acquisition was evaluated, showing that videos, lectures, and printed materials highly contributed. The training videos were rated as more necessary than the simulators for skill acquisition. Psychological evaluation showed that 84% of students experienced anxiety before practice. A positive correlation was found between anxiety levels and frequency of use for all three tools, and students reported that all tools were effective in reducing anxiety during practice. All techniques showed high proficiency rates. Our findings suggest that integrating alternative tools with live-animal training promotes technical skill acquisition, enhances psychological readiness, and supports 3Rs-based laboratory animal practice.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"90-98"},"PeriodicalIF":1.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12826564/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145112288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plenary Lecture 3. 全体讲座3。

IF 2 4区农林科学

Experimental Animals Pub Date : 2026-01-01 DOI: 10.1538/expanim.75suppl-PL3

引用次数: 0

Early-stage therapeutic efficacy of TNAP inhibition using a novel milder murine model of CKD-MBD. 用一种新型轻度CKD-MBD小鼠模型研究TNAP抑制的早期治疗效果。

IF 1.2 4区农林科学

Experimental Animals Pub Date : 2026-01-01 Epub Date: 2025-09-02 DOI: 10.1538/expanim.25-0065

Kaori Soma, José Luis Millán, Anthony Pinkerton, Masanori Izumi

{"title":"Early-stage therapeutic efficacy of TNAP inhibition using a novel milder murine model of CKD-MBD.","authors":"Kaori Soma, José Luis Millán, Anthony Pinkerton, Masanori Izumi","doi":"10.1538/expanim.25-0065","DOIUrl":"10.1538/expanim.25-0065","url":null,"abstract":"Chronic kidney disease (CKD) is a complicated systemic disease displaying various pathophysiological symptoms including mineral bone disorder (CKD-MBD). Ideally, early intervention for CKD-MBD would be desirable, however, there is not enough evidence regarding treatment of CKD-MBD, especially in its early stages, due to its multifactorial pathophysiology and the difficulty in generating adequate animal models. In this study, we evaluated the efficacy of a tissue nonspecific alkaline phosphatase (TNAP) inhibitor, SBI-425 in a CKD-MBD animal model, produced by a combination of nephrectomy and high inorganic phosphate (Pi) diet. This combination induced renal damage, and significantly elevated blood urea nitrogen (BUN). Plasma levels of fibroblast growing factor 23 (FGF-23), parathyroid hormone (PTH) and phosphate were also elevated, leading to ectopic calcification in the kidneys, particularly in the renal tubules. We orally administered SBI-425 twice daily for 12 weeks at doses of 1 and 10 mg/kg, and this treatment significantly inhibited the progression of calcium deposition in the renal tubules. Furthermore, SBI-425 effectively prevented the deterioration of plasma parameters, BUN, FGF-23, PTH, and phosphate. In conclusion, our findings suggest that TNAP inhibition can effectively slow the progression of CKD-MBD by inhibiting the calcification in the renal tubules. These results may have implications for better clinical care of patients with CKD.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"73-80"},"PeriodicalIF":1.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12826570/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144992076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0