Experimental Animals最新文献_第8页

Comparison of retinal parameters between rhesus and cynomolgus macaques. 恒河猴与猕猴视网膜参数的比较。

IF 2.4 4区农林科学

Experimental Animals Pub Date : 2024-02-14 Epub Date: 2023-07-18 DOI: 10.1538/expanim.22-0022

Chengjie He, Jingyi Peng, Jiayi Jin, Wanwen Shao, Yongxin Zheng, Liuxueying Zhong

{"title":"Comparison of retinal parameters between rhesus and cynomolgus macaques.","authors":"Chengjie He, Jingyi Peng, Jiayi Jin, Wanwen Shao, Yongxin Zheng, Liuxueying Zhong","doi":"10.1538/expanim.22-0022","DOIUrl":"10.1538/expanim.22-0022","url":null,"abstract":"Nonhuman primates are important research models for basic vision research, preclinical pathogenesis, and treatment studies due to strong similarities in retinal structure and function with humans. We compared retinal parameters between 10 healthy normal rhesus macaques (Macaca mulatta) and 10 cynomolgus macaques (Macaca fascicularis) by optical coherence tomography and electroretinography. The Heidelberg Spectralis® HRA+OCT and Roland multifocal electrophysiometer were used to analyze retinal morphology, multifocal electroretinograms (mfERGs), and full-field electroretinograms (ff-ERGs). Mean retinal thickness was lowest in the central fovea of macaques and did not differ significantly between species, but the retinal thicknesses of the nerve fiber ganglion cell layer and the inner plexiform layer were significantly different. The amplitude density of the N1 wave was lower in rhesus macaques than in cynomolgus macaques in ring and quadrant areas. Dark-adapted 3.0 oscillatory potentials (reflection of amacrine cell activity) and light-adapted 30-hz flicker ERG (a sensitive cone-pathway-driven response) waveforms of the ff-ERG were similar in both species, while the times to peaks in dark-adapted 0.01 ERG (the rod-driven response of bipolar cells) and dark-adapted 3.0 ERG (combined rod and cone system responses) as well as the implicit times of the a- and b-waves in light-adapted 3.0 ERG (the single-flash cone response) were substantially different. This study provides normative retinal parameters for nonhuman primate research on basic and clinical ophthalmology, as well as a reference for researchers in the appropriate selection of rhesus or cynomolgus macaques as models for ophthalmology studies.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"20-28"},"PeriodicalIF":2.4,"publicationDate":"2024-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10877147/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10185806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Renal transcriptome analysis of uninephrectomized db/db mice identified a mechanism for the transition to severe diabetic nephropathy. 对未切除肾脏的 db/db 小鼠的肾脏转录组分析确定了向严重糖尿病肾病转变的机制。

IF 2.4 4区农林科学

Experimental Animals Pub Date : 2024-02-14 Epub Date: 2023-07-21 DOI: 10.1538/expanim.22-0168

Mariko Maekawa, Tatsuya Maekawa, Tomohiko Sasase, Takeshi Wakashima, Atsuhiro Uemura, Kinuko Uno, Takeshi Ohta, Takahisa Yamada

{"title":"Renal transcriptome analysis of uninephrectomized db/db mice identified a mechanism for the transition to severe diabetic nephropathy.","authors":"Mariko Maekawa, Tatsuya Maekawa, Tomohiko Sasase, Takeshi Wakashima, Atsuhiro Uemura, Kinuko Uno, Takeshi Ohta, Takahisa Yamada","doi":"10.1538/expanim.22-0168","DOIUrl":"10.1538/expanim.22-0168","url":null,"abstract":"Diabetic nephropathy (DN), included in diabetic kidney disease (DKD), is a primary driver of end-stage renal disease (ESRD) leading to dialysis treatment. To develop new therapeutic drugs to prevent ESRD and avoid dialysis treatment, insight into DKD pathophysiology and animal models suitable for drug efficacy testing are needed. In this study, transcriptome analysis of kidneys from 26-week-old and 35-week-old uninephrectomized (UNX) db/db mice was used to identify the pathways that affect the deterioration of renal function in db/db mice. Differentially expressed genes suggested that there was increased interferon (IFN)-γ signaling during the 26 to 35-week period. Modules that changed between 26 and 35 weeks of age extracted by weighted gene co-expression network analysis (WGCNA) suggested increased the tumor necrosis factor (TNF)-α and nuclear factor-kappa B (NF-κB) signaling pathway in component cells of glomeruli. The protein-protein interaction (PPI) network analysis identified Cxcl16 as a hub gene for those signaling pathways, and it was shown that the pathways in this module changed when the glomerular filtration rate decreased in patients with DN. These results suggested the possibility that signaling mediated by Cxcl16 induced by IFN-γ and TNF-α between 26 and 35 weeks of age leads to renal fibrosis, resulting in severe disease. Drugs that target such pathways can be options for developing drugs for DN. We also think that the uninephrectomized db/db mouse can be used as an animal model of severe DKD and to evaluate efficacy in patients with DN.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"29-40"},"PeriodicalIF":2.4,"publicationDate":"2024-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10877145/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9860272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Null mutation of exocyst complex component 3-like does not affect vascular development in mice. 外囊复合体 3-like 成分的无效突变不会影响小鼠的血管发育。

IF 2.4 4区农林科学

Experimental Animals Pub Date : 2024-02-14 Epub Date: 2023-09-01 DOI: 10.1538/expanim.23-0105

Satsuki Takashima, Eiichi Okamura, Yusuke Ichiyama, Kiyoto Nishi, Akio Shimizu, Chisato Watanabe, Masanaga Muto, Shoma Matsumoto, Setsuko Tsukiyama-Fujii, Tomoyuki Tsukiyama, Hisakazu Ogita, Eiichiro Nishi, Masahito Ohji, Fumihiro Sugiyama, Satoru Takahashi, Seiya Mizuno, Ken-Ichi Mizutani, Masatsugu Ema

{"title":"Null mutation of exocyst complex component 3-like does not affect vascular development in mice.","authors":"Satsuki Takashima, Eiichi Okamura, Yusuke Ichiyama, Kiyoto Nishi, Akio Shimizu, Chisato Watanabe, Masanaga Muto, Shoma Matsumoto, Setsuko Tsukiyama-Fujii, Tomoyuki Tsukiyama, Hisakazu Ogita, Eiichiro Nishi, Masahito Ohji, Fumihiro Sugiyama, Satoru Takahashi, Seiya Mizuno, Ken-Ichi Mizutani, Masatsugu Ema","doi":"10.1538/expanim.23-0105","DOIUrl":"10.1538/expanim.23-0105","url":null,"abstract":"Exocyst is an octameric protein complex implicated in exocytosis. The exocyst complex is highly conserved among mammalian species, but the physiological function of each subunit in exocyst remains unclear. Previously, we identified exocyst complex component 3-like (Exoc3l) as a gene abundantly expressed in embryonic endothelial cells and implicated in the process of angiogenesis in human umbilical cord endothelial cells. Here, to reveal the physiological roles of Exoc3l during development, we generated Exoc3l knockout (KO) mice by genome editing with CRISPR/Cas9. Exoc3l KO mice were viable and showed no significant phenotype in embryonic angiogenesis or postnatal retinal angiogenesis. Exoc3l KO mice also showed no significant alteration in cholesterol homeostasis or insulin secretion, although several reports suggest an association of Exoc3l with these processes. Despite the implied roles, Exoc3l KO mice exhibited no apparent phenotype in vascular development, cholesterol homeostasis, or insulin secretion.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"93-100"},"PeriodicalIF":2.4,"publicationDate":"2024-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10877151/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10202084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Middle aged CAMKII-Cre:Cbs^fl/fl mice: a new model for studying perioperative neurocognitive disorders. 中年CAMKII-Cre:CBSfl/fl小鼠：研究围手术期神经认知障碍的新模型。

IF 2.2 4区农林科学

Experimental Animals Pub Date : 2024-02-14 Epub Date: 2023-09-27 DOI: 10.1538/expanim.23-0065

Zhen Li, Mengfan He, Danqing Dai, Xiaofei Gao, Huazheng Liang, Lize Xiong

{"title":"Middle aged CAMKII-Cre:Cbsfl/fl mice: a new model for studying perioperative neurocognitive disorders.","authors":"Zhen Li, Mengfan He, Danqing Dai, Xiaofei Gao, Huazheng Liang, Lize Xiong","doi":"10.1538/expanim.23-0065","DOIUrl":"10.1538/expanim.23-0065","url":null,"abstract":"Postoperative complications, such as perioperative neurocognitive disorders (PND), have become a major issue affecting surgical outcomes. However, the mechanism of PND remains unclear, and stable animal models of middle-aged PND are lacking. S-adenosylmethionine (SAM), a cystathionine beta-synthase (CBS) allosteric activator, can reduce the level of plasma homocysteine and prevent the occurrence of PND. However, the time and resource-intensive process of constructing models of PND in elderly animals have limited progress in PND research and innovative therapy development. The present study aimed to construct a stable PND model in middle-aged CAMKII-Cre:Cbsfl/fl mice whose Cbs was specifically knocked out in CAMKII positive neurons. Behavioral tests showed that these middle-aged mice displayed cognitive deficits which were aggravated by exploratory laparotomy under isoflurane anesthesia. Compared with typical PND mice which were 18-month-old, these middle-aged mice showed similar cognitive deficits after undergoing exploratory laparotomy under isoflurane anesthesia. Though there was no significant difference in the number of neurons in either the hippocampus or the cortex, a significant increase in numbers of microglia and astrocytes in the hippocampus was observed. These indicate that middle-aged CAMKII-Cre:Cbsfl/fl mice can be used as a new PND model for mechanistic studies and therapy development for PND.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"109-123"},"PeriodicalIF":2.2,"publicationDate":"2024-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10877146/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41096860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

L-lysine supplementation attenuates experimental autoimmune hepatitis in a chronic murine model. 补充 L-赖氨酸可减轻慢性小鼠模型中的实验性自身免疫性肝炎。

IF 2.4 4区农林科学

Experimental Animals Pub Date : 2024-02-14 Epub Date: 2023-08-31 DOI: 10.1538/expanim.23-0053

Yu Lei, Yu Chen, Shuhui Wang, Zhuoying Lin, Ping Han, Dean Tian, Han Wang, Mei Liu

{"title":"L-lysine supplementation attenuates experimental autoimmune hepatitis in a chronic murine model.","authors":"Yu Lei, Yu Chen, Shuhui Wang, Zhuoying Lin, Ping Han, Dean Tian, Han Wang, Mei Liu","doi":"10.1538/expanim.23-0053","DOIUrl":"10.1538/expanim.23-0053","url":null,"abstract":"The incidence of autoimmune hepatitis (AIH) has increased significantly worldwide. The present study aims to explore the protective effect of L-lysine supplementation against AIH and to investigate its potential underlying mechanisms. A chronic experimental AIH mouse model was established by repeated tail vein injection of human cytochrome P450 2D6 (CYP2D6) plasmid. Starting from day 14 of the modeling, mice in the CYP2D6-AIH +L-lysine group were given 200 µl of purified water containing 10 mg/kg L-lysine by gavage until day27, once a day, and mice in the healthy control group and model group were given an equal volume of purified water by gavage. Our results showed that L-lysine supplementation partially reversed the liver injury mediated by CYP2D6 overexpression. These effects were consistent with the restraining impacts of L-lysine supplementation on decreasing pro-inflammatory cytokines expression level and CD4+ and CD8+ T lymphocytes infiltration, as well as curbing hepatic oxidative stress. Furthermore, L-lysine supplement relieved liver fibrosis in the context of AIH. In conclusion, L-lysine supplementation attenuates CYP2D6-induced immune liver injury in mice, which may serve as a novel nutrition support approach for AIH.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"83-92"},"PeriodicalIF":2.4,"publicationDate":"2024-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10877156/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10176966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differences in exercise capacity and muscle glycogen metabolism in C57BL/6J and BALB/cA mice. C57BL/6J 和 BALB/cA 小鼠运动能力和肌糖原代谢的差异。

IF 2.4 4区农林科学

Experimental Animals Pub Date : 2024-02-14 Epub Date: 2023-09-12 DOI: 10.1538/expanim.23-0074

Tohru Miyata, Akira Shogatsudani, Ayaka Igarashi, Haruna Tsutiya, Kyouka Yoshida

{"title":"Differences in exercise capacity and muscle glycogen metabolism in C57BL/6J and BALB/cA mice.","authors":"Tohru Miyata, Akira Shogatsudani, Ayaka Igarashi, Haruna Tsutiya, Kyouka Yoshida","doi":"10.1538/expanim.23-0074","DOIUrl":"10.1538/expanim.23-0074","url":null,"abstract":"This study compared differences in exercise capacity as well as muscle glycogen content and degradation, and mitochondrial enzyme activity between C57BL/6J and BALB/cA mice. In exercise tests, grip strength was higher in BALB/cA mice. In Rotarod and Inverted screen test, C57BL/6J mice had significantly longer exercise durations and showed differences in motor function and muscle endurance time. Glycogen in the liver and muscle of C57BL/6J mice was significantly decreased after 20 min of swimming. Muscle glycogen content in BALB/cA mice was higher than in C57BL/6J, but swimming induced no decrease in glycogen content. Glycogen phosphorylase in muscle was inactive in the absence of AMP, and its activity increased in a concentration-dependent manner with the addition of AMP in C57BL/6J mice. In BALB/cA mice, phosphorylase activity was increased by AMP, but not further increased by higher concentrations of AMP. The citrate synthase activity in muscle did not differ between C57BL/6J and BALB/cA mice. The results of this study suggested that the reactivity of muscle glycogen phosphorylase to AMP differs among strains of mice and affects glycogen availability during exercise.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"101-108"},"PeriodicalIF":2.4,"publicationDate":"2024-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10877153/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10222345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cardiorenal damages in mice at early phase after intervention induced by angiotensin II, nephrectomy, and salt intake. 血管紧张素 II、肾切除术和食盐摄入诱导干预后早期小鼠的心肾损害。

IF 2.4 4区农林科学

Experimental Animals Pub Date : 2024-02-14 Epub Date: 2023-07-18 DOI: 10.1538/expanim.23-0071

Naoto Muromachi, Junji Ishida, Kazuyuki Noguchi, Tomoki Akiyama, Syunsuke Maruhashi, Kaori Motomura, Joichi Usui, Kunihiro Yamagata, Akiyoshi Fukamizu

{"title":"Cardiorenal damages in mice at early phase after intervention induced by angiotensin II, nephrectomy, and salt intake.","authors":"Naoto Muromachi, Junji Ishida, Kazuyuki Noguchi, Tomoki Akiyama, Syunsuke Maruhashi, Kaori Motomura, Joichi Usui, Kunihiro Yamagata, Akiyoshi Fukamizu","doi":"10.1538/expanim.23-0071","DOIUrl":"10.1538/expanim.23-0071","url":null,"abstract":"The interconnection of heart performance and kidney function plays an important role for maintaining homeostasis through a variety of physiological crosstalk between these organs. It has been suggested that acute or chronic dysfunction in one organ causes dysregulation in another one, like patients with cardiorenal syndrome. Despite its growing recognition as global health issues, still little is known on pathophysiological evaluation between the two organs. Previously, we established a preclinical murine model with cardiac hypertrophy and fibrosis, and impaired kidney function with renal enlargement and increased urinary albumin levels induced by co-treatment with vasopressor angiotensin II (A), unilateral nephrectomy (N), and salt loading (S) (defined as ANS treatment) for 4 weeks. However, how both tissues, heart and kidney, are initially affected by ANS treatment during the progression of tissue damages remains to be determined. Here, at one week after ANS treatment, we found that cardiac function in ANS-treated mice (ANS mice) are sustained despite hypertrophy. On the other hand, kidney dysfunction is evident in ANS mice, associated with high blood pressure, enlarged glomeruli, increased levels of urinary albumin and urinary neutrophil gelatinase-associated lipocalin, and reduced creatinine clearance. Our results suggest that cardiorenal tissues become damaged at one week after ANS treatment and that ANS mice are useful as a model causing transition from early to late-stage damages of cardiorenal tissues.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"11-19"},"PeriodicalIF":2.4,"publicationDate":"2024-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10877154/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9882070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nucleic acid sensing Toll-like receptors 3 and 9 play complementary roles in the development of bacteremia after nasal colonization associated with influenza co-infection. 核酸感应 Toll 样受体 3 和 9 在与流感合并感染相关的鼻腔定植后发生菌血症的过程中发挥互补作用。

IF 2.2 4区农林科学

Experimental Animals Pub Date : 2024-02-14 Epub Date: 2023-08-03 DOI: 10.1538/expanim.23-0001

Denisa Nanushaj, Masamitsu Kono, Hideki Sakatani, Daichi Murakami, Muneki Hotomi

{"title":"Nucleic acid sensing Toll-like receptors 3 and 9 play complementary roles in the development of bacteremia after nasal colonization associated with influenza co-infection.","authors":"Denisa Nanushaj, Masamitsu Kono, Hideki Sakatani, Daichi Murakami, Muneki Hotomi","doi":"10.1538/expanim.23-0001","DOIUrl":"10.1538/expanim.23-0001","url":null,"abstract":"Streptococcus pneumoniae can cause mortality in infant, elderly, and immunocompromised individuals owing to invasion of bacteria to the lungs, the brain, and the blood. In building strategies against invasive infections, it is important to achieve greater understanding of how the pneumococci are able to survive in the host. Toll-like receptors (TLRs), critically important components in the innate immune system, have roles in various stages of the development of infectious diseases. Endosomal TLRs recognize nucleic acids of the pathogen, but the impact on the pneumococcal diseases of immune responses from signaling them remains unclear. To investigate their role in nasal colonization and invasive disease with/without influenza co-infection, we established a mouse model of invasive pneumococcal diseases directly developing from nasal colonization. TLR9 KO mice had bacteremia more frequently than wildtype in the pneumococcal mono-infection model, while the occurrence of bacteremia was higher among TLR3 KO mice after infection with influenza in advance of pneumococcal inoculation. All TLR KO strains showed poorer survival than wildtype after the mice had bacteremia. The specific and protective role of TLR3 and TLR9 was shown in developing bacteremia with/without influenza co-infection respectively, and all nucleic sensing TLRs would contribute equally to protecting sepsis after bacteremia.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"50-60"},"PeriodicalIF":2.2,"publicationDate":"2024-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10877144/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9929353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Eriocalyxin B alleviated ischemic cerebral injury by limiting microglia-mediated excessive neuroinflammation in mice. Eriocalycin B通过限制小胶质细胞介导的小鼠过度神经炎症来减轻缺血性脑损伤。

IF 2.4 4区农林科学

Experimental Animals Pub Date : 2024-02-14 Epub Date: 2023-10-14 DOI: 10.1538/expanim.23-0070

Yanqiang Chen, Cong Zhang, Liming Zhao, Rong Chen, Peipei Zhang, Junxia Li, Xueping Zhang, Xiangjian Zhang

{"title":"Eriocalyxin B alleviated ischemic cerebral injury by limiting microglia-mediated excessive neuroinflammation in mice.","authors":"Yanqiang Chen, Cong Zhang, Liming Zhao, Rong Chen, Peipei Zhang, Junxia Li, Xueping Zhang, Xiangjian Zhang","doi":"10.1538/expanim.23-0070","DOIUrl":"10.1538/expanim.23-0070","url":null,"abstract":"Excessive neuroinflammation mediated by microglia has a detrimental effect on the progression of ischemic stroke. Eriocalyxin B (EriB) was found with a neuroprotective effect in mice with Parkinson's disease via the suppression of microglial overactivation. This study aimed to investigate the roles of EriB in permanent middle cerebral artery occlusion (pMCAO) mice. The pMCAO was induced in the internal carotid artery of the mice by the intraluminal filament method, and EriB (10 mg/kg) was administered immediately after surgery by intraperitoneal injection. The behavior score, 2,3,5-triphenyltetrazole chloride staining, Nissl staining, TUNEL, immunohistochemistry, immunofluorescence, PCR, ELISA, and immunoblotting revealed that EriB administration reduced brain infarct and neuron death and ameliorated neuroinflammation and microglia overactivation in pMCAO mice, manifested by alterations of TUNEL-positive cell numbers, ionized calcium binding adaptor molecule 1 (Iba-1)-positive cell numbers, and expression of tumor necrosis factor-α, interleukin 6, IL-1β, inducible nitric oxide synthase, and arginase 1. In addition, EriB suppressed ischemia-induced activation of nuclear factor kappa B (NF-κB) signaling in the brain penumbra, suggesting the involvement of NF-κB in EriB function. In conclusion, EriB exerted anti-inflammatory effects in ischemia stroke by regulating the NF-κB signaling pathway, and this may provide insights into the neuroprotective effect of EriB in the treatment of ischemic stroke.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"124-135"},"PeriodicalIF":2.4,"publicationDate":"2024-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10877152/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41233601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Errata: Middle aged CAMKII-Cre:Cbs^fl/fl mice: a new model for studying perioperative neurocognitive disorders. 勘误：中老年 CAMKII-Cre:Cbsfl/fl 小鼠：研究围手术期神经认知障碍的新模型

IF 2.2 4区农林科学

Experimental Animals Pub Date : 2024-01-01 DOI: 10.1538/expanim.23-0065e

{"title":"Errata: Middle aged CAMKII-Cre:Cbsfl/fl mice: a new model for studying perioperative neurocognitive disorders.","authors":"","doi":"10.1538/expanim.23-0065e","DOIUrl":"10.1538/expanim.23-0065e","url":null,"abstract":"Zhen Li, Mengfan He, Danqing Dai, Xiaofei Gao, Huazheng Liang and Lize Xiong Exp. Anim. 73(1), 109-123, 2024 https://doi.org/10.1538/expanim.23-0065 In the original publication of the article, the Funding section was incomplete. The correct Funding information is provided below: Funding This work was supported by a grant from the Shanghai Commission of Science and Technology (201409003500), Major Project of National Natural Science Foundation of China (No. 82293640, No. 82293643), Key Project of National Natural Science Foundation of China (No. 82130121), the second round of the three-year action plan for \"strengthening and promoting Traditional Chinese Medicine\" of Hongkou District (HKGYQYXM-2022-06), and Scientific and technological innovation 2030 - major project of Brain Science and Brain-Like Intelligence Technology (2021ZD0202804) to Dr. Lize Xiong, a grant from the National Natural Science Foundation of China (No.81974535) to Dr. Huazheng Liang, and the Talent Promotion Program of Shanghai Fourth People's Hospital Affiliated to Tongji University School of Medicine (SY-XKZT-2019-3006) and the Discipline Promotion Program of Shanghai Fourth People's Hospital Affiliated to Tongji University School of Medicine (SY-XKZT-2019-1012) to Dr. Zhen Li.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":"73 2","pages":"233"},"PeriodicalIF":2.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11091357/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140862635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0