Experimental Animals最新文献_第9页

Effect of felodipine on indomethacin-induced gastric ulcers in rats. 非洛地平对消炎痛致大鼠胃溃疡的影响。

IF 2.4 4区农林科学

Experimental Animals Pub Date : 2023-11-09 Epub Date: 2023-06-13 DOI: 10.1538/expanim.23-0052

Nergis Akbaş, Bahadır Süleyman, Renad Mammadov, Mine Gülaboğlu, Emin Murat Akbaş, Halis Süleyman

{"title":"Effect of felodipine on indomethacin-induced gastric ulcers in rats.","authors":"Nergis Akbaş, Bahadır Süleyman, Renad Mammadov, Mine Gülaboğlu, Emin Murat Akbaş, Halis Süleyman","doi":"10.1538/expanim.23-0052","DOIUrl":"10.1538/expanim.23-0052","url":null,"abstract":"Felodipine is a calcium channel blocker with antioxidant and anti-inflammatory properties. Researchers have stated that oxidative stress and inflammation also play a role in the pathophysiology of gastric ulcers caused by nonsteroidal anti-inflammatory drugs. The aim of this study was to investigate the antiulcer effect of felodipine on indomethacin-induced gastric ulcers in Wistar rats and compare it with that of famotidine. The antiulcer activities of felodipine (5 mg/kg) and famotidine were investigated biochemically and macroscopically in animals treated with felodipine (5 mg/kg) and famotidine in combination with indomethacin. The results were compared with those of the healthy control group and the group administered indomethacin alone. It was observed that felodipine suppressed the indomethacin-induced malondialdehyde increase (P<0.001); reduced the decrease in total glutathione amount (P<0.001), reduced the decrease superoxide dismutase (P<0.001), and catalase activities (P<0.001); and significantly inhibited ulcers (P<0.001) at the tested dose compared with indomethacin alone. Felodipine at a dose of 5 mg/kg reduced the indomethacin-induced decrease in cyclooxygenase-1 activity (P<0.001) but did not cause a significant reduction in the decrease in cyclooxygenase-2 activity. The antiulcer efficacy of felodipine was demonstrated in this experimental model. These data suggest that felodipine may be useful in the treatment of nonsteroidal anti-inflammatory drug-induced gastric injury.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"505-512"},"PeriodicalIF":2.4,"publicationDate":"2023-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10658091/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9636158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Annual two-dose tetanus toxoid vaccination induces protective humoral immunity to all age groups of rhesus macaques. 每年两次破伤风类毒素疫苗接种可对所有年龄组的恒河猴产生保护性体液免疫。

IF 2.4 4区农林科学

Experimental Animals Pub Date : 2023-11-09 Epub Date: 2023-06-06 DOI: 10.1538/expanim.23-0040

Megumi Murata, Anastasiia Kovba, Akihisa Kaneko, Mayumi Morimoto, Akiyo Ishigami, Takayoshi Natsume, Ayaka Washizaki, Takako Miyabe-Nishiwaki, Juri Suzuki, Hirofumi Akari

{"title":"Annual two-dose tetanus toxoid vaccination induces protective humoral immunity to all age groups of rhesus macaques.","authors":"Megumi Murata, Anastasiia Kovba, Akihisa Kaneko, Mayumi Morimoto, Akiyo Ishigami, Takayoshi Natsume, Ayaka Washizaki, Takako Miyabe-Nishiwaki, Juri Suzuki, Hirofumi Akari","doi":"10.1538/expanim.23-0040","DOIUrl":"10.1538/expanim.23-0040","url":null,"abstract":"A tetanus outbreak occurred during 2014-2015 in the rhesus macaques reared in an open enclosure in our facility. As the soil of the facility was suspected to be contaminated with Clostridium tetani spores, there was a risk of further tetanus occurring among the macaques. To protect them from tetanus, a tetanus toxoid vaccination was recommended; however, the vaccinated elderly animals might not be effectively protected due to insufficient humoral immune responses. Hence, we evaluated the dynamics of antibody responses among rhesus macaques of all age groups vaccinated with two-dose tetanus toxoid at a 1-year interval during a 3-year follow-up study. The vaccination developed anti-tetanus toxin-specific antibodies in animals of all age groups, the antibody levels peaked 1 year after the second vaccination, and the peak levels decreased with age. However, the levels among elderly individuals (aged ≥13 years) were still higher than the threshold level, which was supposed to protect them from tetanus development. Although the rhesus macaques in our facility had a risk of occasional exposure to the spores due to the outbreak, no incidence of tetanus has ever occurred to date. These results indicate that the vaccination protocol is effective in protecting not only younger but also older animals from tetanus.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"490-495"},"PeriodicalIF":2.4,"publicationDate":"2023-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10658093/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9583648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Humanized CD36 (hCD36) mouse model supports the preclinical evaluation of therapeutic candidates targeting CD36. 人源化CD36 (hCD36)小鼠模型支持靶向CD36的候选治疗方案的临床前评估。

IF 2.4 4区农林科学

Experimental Animals Pub Date : 2023-11-09 Epub Date: 2023-07-06 DOI: 10.1538/expanim.23-0021

Xiulong Xie, Zhenlan Niu, Linlin Wang, Xiaofei Zhou, Xingyan Yu, Hongyan Jing, Yi Yang

{"title":"Humanized CD36 (hCD36) mouse model supports the preclinical evaluation of therapeutic candidates targeting CD36.","authors":"Xiulong Xie, Zhenlan Niu, Linlin Wang, Xiaofei Zhou, Xingyan Yu, Hongyan Jing, Yi Yang","doi":"10.1538/expanim.23-0021","DOIUrl":"10.1538/expanim.23-0021","url":null,"abstract":"CD36 (also known as scavenger receptor B2) is a multifunctional receptor that mediates lipid uptake, advanced oxidation protein products, and immunological recognition, and has roles in lipid accumulation, apoptosis, as well as in metastatic colonization in cancer. CD36 is involved in tumor immunity, metastatic invasion, and therapy resistance through various molecular mechanisms. Targeting CD36 has emerged as an effective strategy for tumor immunotherapy. In this study, we have successfully generated a novel hCD36 mouse (Unless otherwise stated, hCD36 mouse below refer to homozygous hCD36 mouse) strain where the sequences encoding the extracellular domains of the mouse Cd36 gene were replaced with the corresponding human sequences. The results showed that the hCD36 mice only expressed human CD36, and the proportion of each lymphocyte was not significantly changed compared with wild-type mice. Furthermore, CD36 monoclonal antibody could significantly inhibit tumor growth after treatment. Therefore, the hCD36 mouse represent a validated preclinical mouse model for the evaluation of tumor immunotherapy targeting CD36.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"535-545"},"PeriodicalIF":2.4,"publicationDate":"2023-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10658083/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9752575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Apolipoprotein E-depletion accelerates arterial fat deposition in the spontaneously hypertensive rat. 载脂蛋白e耗竭加速自发性高血压大鼠动脉脂肪沉积。

IF 2.4 4区农林科学

Experimental Animals Pub Date : 2023-11-09 Epub Date: 2023-04-20 DOI: 10.1538/expanim.23-0012

Hiroyuki Matsuo, Kohei Kawakami, Hiroki Ohara, Takehito Kaneko, Tomoji Mashimo, Takaya Yamada, Toru Nabika

{"title":"Apolipoprotein E-depletion accelerates arterial fat deposition in the spontaneously hypertensive rat.","authors":"Hiroyuki Matsuo, Kohei Kawakami, Hiroki Ohara, Takehito Kaneko, Tomoji Mashimo, Takaya Yamada, Toru Nabika","doi":"10.1538/expanim.23-0012","DOIUrl":"10.1538/expanim.23-0012","url":null,"abstract":"Hypertension and atherosclerosis are often found in one patient causing serious cardiovascular events. An animal model simultaneously expressing hypertension and atherosclerosis would be useful to study such a complex risk status. We therefore attempted to introduce a null mutation of the apolipoprotein E (ApoE) gene into the spontaneously hypertensive rat (SHR) using CRISPR/Cas9 to establish a genetic model for atherosclerosis with hypertension. We successfully established SHRApoE(-/-) having a 13-bps deletion in the 5'-end of ApoE gene. Deletion of ApoE protein was confirmed by Western blotting. Blood pressure of SHRApoE(-/-) was comparable to that of SHR. Feeding the rats with high fat high cholesterol diet (HFD) caused a significant increase in LDL cholesterol as well as in triglyceride in SHRApoE(-/-). After 8 weeks of HFD loading, superficial fat deposition was observed both in the aorta and the mesenteric arteries of SHRApoE(-/-) instead of mature atheromatous lesions found in humans. In addition, a null mutation of peroxiredoxin 2 (Prdx2) was introduced into SHRApoE(-/-) to examine the effect of increased oxidative stress on the development of atherosclerosis. SHR with the double depletion of ApoE and Prdx2 did not show mature atheroma either. Further, salt loading did not promote development of atheroma although it accelerated the development of fat deposition. These results indicated that when compared with ApoE-knockout mice, SHRApoE(-/-) was more resistant to atherosclerosis even though they have severe hypertension.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"439-445"},"PeriodicalIF":2.4,"publicationDate":"2023-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10658095/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9790544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Targeted proteomic analysis reveals that crocodile oil from Crocodylus siamensis may enhance hepatic energy metabolism in rats. 目标蛋白质组学分析表明，鳄鱼油可促进大鼠肝脏能量代谢。

IF 2.4 4区农林科学

Experimental Animals Pub Date : 2023-11-09 Epub Date: 2023-04-07 DOI: 10.1538/expanim.23-0009

Wirasak Fungfuang, Krittika Srisuksai, Pitchaya Santativongchai, Sawanya Charoenlappanit, Narumon Phaonakrop, Sittiruk Roytrakul, Phitsanu Tulayakul, Kongphop Parunyakul

{"title":"Targeted proteomic analysis reveals that crocodile oil from Crocodylus siamensis may enhance hepatic energy metabolism in rats.","authors":"Wirasak Fungfuang, Krittika Srisuksai, Pitchaya Santativongchai, Sawanya Charoenlappanit, Narumon Phaonakrop, Sittiruk Roytrakul, Phitsanu Tulayakul, Kongphop Parunyakul","doi":"10.1538/expanim.23-0009","DOIUrl":"10.1538/expanim.23-0009","url":null,"abstract":"The liver is a key organ governing body energy metabolism. Dietary fats influence energy metabolism and mitochondrial functioning. Crocodile oil (CO) is rich in mono- and polyunsaturated fatty acids that contain natural anti-inflammatory and healing properties. Our study examined how CO affects the expressions of liver proteins involved in energy metabolism in rats. Twenty-one male Sprague Dawley rats were divided into three groups and underwent oral gavage with 3 ml/kg of sterile water (N group), CO (CO group), or palm oil (PO group) for 7 weeks. Body weight, energy intake, liver weight, liver indexes, blood lipid profiles, and liver-energy intermediates were measured. The liver proteome was analyzed using shotgun proteomics, and the functions and network interactions of several candidate proteins were predicted using the STITCH v.5.0 software. Body weights, energy intake, liver contents, and lipid profiles did not differ between the groups. However, hepatic oxaloacetate and malate levels were significantly higher in the CO group than in the PO group. Targeted proteomics reveals that 22 out of 1,790 unique proteins in the CO group were involved in energy-generating pathways, including the tricarboxylic acid cycle and oxidative phosphorylation (OXPHOS), and were correlated with the AMP-activated protein kinase signaling pathway. Cluster analysis of 59 differentially expressed proteins showed that OXPHOS-associated proteins were upregulated in the CO group and that three glycolytic metabolism-related proteins were downregulated in the CO group. CO may enhance hepatic energy metabolism by regulating the expressions of energy expenditure-related proteins.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"425-438"},"PeriodicalIF":2.4,"publicationDate":"2023-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10658085/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9318537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Intraplacental injection of AAV9-CMV-iCre results in the widespread transduction of multiple organs in double-reporter mouse embryos. 胎盘内注射AAV9-CMV-iCre可导致双报告小鼠胚胎中多个器官的广泛转导。

IF 2.4 4区农林科学

Experimental Animals Pub Date : 2023-11-09 Epub Date: 2023-05-12 DOI: 10.1538/expanim.23-0044

Natalia Gogoleva, Zeynab Javanfekr Shahri, Atsushi Noda, Ching-Wei Liao, Arata Wakimoto, Yuri Inoue, Hyojung Jeon, Satoru Takahashi, Michito Hamada

引用次数: 0

Establishment of a novel experimental system using single cell-derived pleomorphic rhabdomyosarcoma cell lines expressing K-RasG12V and deficient in p53. 利用表达K-RasG12V和p53缺失的单细胞衍生多形性横纹肌肉瘤细胞系建立新的实验系统。

IF 2.4 4区农林科学

Experimental Animals Pub Date : 2023-11-09 Epub Date: 2023-04-19 DOI: 10.1538/expanim.22-0177

Hiromitsu Saito, Noboru Suzuki

{"title":"Establishment of a novel experimental system using single cell-derived pleomorphic rhabdomyosarcoma cell lines expressing K-RasG12V and deficient in p53.","authors":"Hiromitsu Saito, Noboru Suzuki","doi":"10.1538/expanim.22-0177","DOIUrl":"10.1538/expanim.22-0177","url":null,"abstract":"Pleomorphic rhabdomyosarcoma (PRMS) predominantly arises in adult skeletal musculature and is usually associated with poor prognosis. Thus, effective treatments must be developed. PRMS is a rare tumor; therefore, it is critical to develop an experimental system to understand the cellular and molecular mechanisms of PRMS. We previously demonstrated that PRMS develops after p53 gene deletion and oncogenic K-Ras expression in the skeletal muscle tissue. In that study, oncogenic K-Ras-expressing cells were diverse and the period until disease onset was difficult to control. In this study, we developed an experimental system to address this problem. Single cell-derived murine cell lines, designated as RMS310 and RMSg2, were established by limiting the dilution of cells from a lung metastatic tumor colony that were positive for various cancer stem cells and activated skeletal muscle-resident stem/progenitor cell marker genes by RT-PCR. All cell lines stably recapitulated the histological characteristics of human PRMS as bizarre giant cells, desmin-positive cells, and lung metastases in C57BL/6 mice. All subclones of the RMSg2 cells by the limiting dilution in vitro could seed PRMS subcutaneously, and as few as 500 RMSg2 cells were sufficient to form tumors. These results suggest that the RMSg2 cells are multipotent cancer cells that partially combine the properties of skeletal muscle-resident stem/progenitor cells and high tumorigenicity. Thus, our model system's capacity to regenerate tumor tissue in vivo and maintain stable cells in vitro makes it useful for developing therapeutics to treat PRMS.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"446-453"},"PeriodicalIF":2.4,"publicationDate":"2023-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10658087/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9476812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Administration of lipid emulsion reduced the hypnotic potency of propofol more than that of thiamylal in mice. 脂质乳剂对异丙酚的催眠作用比硫胺醛对小鼠的催眠作用更大。

IF 2.4 4区农林科学

Experimental Animals Pub Date : 2023-11-09 Epub Date: 2023-06-02 DOI: 10.1538/expanim.23-0010

Michiko Higashi, Saori Taharabaru, Yushi U Adachi, Maiko Satomoto, Takahiro Tamura, Naoyuki Matsuda, Aiji Sato-Boku, Masahiro Okuda

{"title":"Administration of lipid emulsion reduced the hypnotic potency of propofol more than that of thiamylal in mice.","authors":"Michiko Higashi, Saori Taharabaru, Yushi U Adachi, Maiko Satomoto, Takahiro Tamura, Naoyuki Matsuda, Aiji Sato-Boku, Masahiro Okuda","doi":"10.1538/expanim.23-0010","DOIUrl":"10.1538/expanim.23-0010","url":null,"abstract":"Administration in a lipid emulsion can modify the pharmacodynamics of drugs via a process known as lipid resuscitation. However, the detailed mechanism remains unclear. We studied the volume and another pharmacodynamic effect, the lipid sink, using propofol and thiamylal. Male adult mice (ddY) were intravenously administered 10 ml/kg propofol or thiamylal diluted with physiological saline, 10% soybean oil, or 20% soybean oil. The 50% effective dose (ED50) for achieving hypnosis was calculated using probit analysis. To investigate the volume effect, 0, 10, or 20 ml/kg of saline or soybean oil was administered, either simultaneously or beforehand. Next, a two- or three-fold dose of the anesthetics was administered and the durations of anesthesia were measured. Finally, at 30 s after the first injection, supplemental soybean oil was administered. The mean (± SE) ED50 values of propofol and thiamylal were 5.79 mg/kg (0.61) and 8.83 mg/kg (0.84), respectively. Lipid dilution increased the ED50 values of both anesthetics. After injection of a dose two-fold the ED50 value, the respective mean (± SD) durations of anesthesia were 125 ± 35 s and 102 ± 38 s. Supplemental administration of soybean oil significantly shortened the duration of anesthesia of propofol, but not that of thiamylal. The results indicate that administration of a lipid emulsion vitiated the anesthetic effect of propofol by reducing the non-emulsified free fraction in the aqueous phase, which may elucidate the lipid resuscitation likely caused by the lipid sink mechanism.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"468-474"},"PeriodicalIF":2.4,"publicationDate":"2023-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10658092/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9572313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differences in susceptibility to ADR nephropathy among C57BL/6 substrains. C57BL/6亚型对不良反应肾病的易感性差异

IF 2.4 4区农林科学

Experimental Animals Pub Date : 2023-11-09 Epub Date: 2023-06-21 DOI: 10.1538/expanim.23-0003

Masaki Watanabe, Momoka Kakutani, Koki Hiura, Hayato Sasaki, Nobuya Sasaki

{"title":"Differences in susceptibility to ADR nephropathy among C57BL/6 substrains.","authors":"Masaki Watanabe, Momoka Kakutani, Koki Hiura, Hayato Sasaki, Nobuya Sasaki","doi":"10.1538/expanim.23-0003","DOIUrl":"10.1538/expanim.23-0003","url":null,"abstract":"Adriamycin (ADR) nephropathy is the most widely used nephropathy model to study the pathophysiological mechanisms of chronic kidney disease (CKD) in mice. However, its application is limited to a few mouse strains such as the BALB/c strain; the standard strain, C57BL/6J (B6J), does not develop ADR nephropathy. Nevertheless, Arif et al. reported that C57BL/6N (B6N), another standard strain, is ADR-susceptible. Since then, no follow-up reports or other studies have been published on ADR nephropathy in B6N mice. Therefore, the goal of this study was to determine whether B6N mice are indeed susceptible to ADR nephropathy and whether there are differences in ADR susceptibility among the substrains of C57BL/6NCrl (NCrl) and C57BL/6NJcl (NJcl). NCrl mice showed marked albuminuria and mesangial cell proliferation, which are associated with mild ADR nephropathy, confirming that NCrl mice were susceptible to ADR nephropathy. On the other hand, NJcl mice did not exhibit these symptoms. ADR nephropathy models are usually generated by administering ADR through the tail vein, but Arif et al. administered ADR through the orbital vein. Therefore, we investigated the effect of the route of administration on ADR nephropathy. The degree of ADR nephropathy was found to vary based on the route of administration: more severe nephropathy was observed upon administration through the tail vein than through the orbital vein. Therefore, we conclude that NCrl mice are susceptible to ADR nephropathy, and the severity of ADR-induced nephropathy through orbital vein administration is relatively lower than that through the tail vein.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"520-525"},"PeriodicalIF":2.4,"publicationDate":"2023-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10658096/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9671396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Generation of reporter mice for detecting the transcriptional activity of nuclear factor of activated T cells. 用于检测活化T细胞核因子转录活性的报告小鼠的产生。

IF 2.4 4区农林科学

Experimental Animals Pub Date : 2023-11-09 Epub Date: 2023-04-27 DOI: 10.1538/expanim.23-0043

Norimasa Yamasaki, Kento Miura, Sawako Ogata, Shuka Miura, Arikuni Uchimura, Yasunari Satoh, Masaaki Toshishige, Naohisa Hosomi, Maribet Gamboa, Noriko Kitamura, Osamu Kaminuma

{"title":"Generation of reporter mice for detecting the transcriptional activity of nuclear factor of activated T cells.","authors":"Norimasa Yamasaki, Kento Miura, Sawako Ogata, Shuka Miura, Arikuni Uchimura, Yasunari Satoh, Masaaki Toshishige, Naohisa Hosomi, Maribet Gamboa, Noriko Kitamura, Osamu Kaminuma","doi":"10.1538/expanim.23-0043","DOIUrl":"10.1538/expanim.23-0043","url":null,"abstract":"Nuclear factor of activated T cells (NFAT) is a transcription factor essential for immunological and other biological responses. To develop analyzing system for NFAT activity in vitro and in vivo, we generated reporter mouse lines introduced with NFAT-driven enhanced green fluorescent protein (EGFP) expressing gene construct. Six tandem repeats of -286 to -265 of the human IL2 gene to which NFAT binds in association with its co-transcription factor, activator protein (AP)-1, was conjunct with thymidine kinase minimum promoter and following EGFP coding sequence. Upon introduction of the resulting reporter cassette into C57BL/6 fertilized eggs, the transgenic mice were obtained. Among 7 transgene-positive mice in 110 mice bone, 2 mice showed the designated reporter mouse character. Thus, the EGFP fluorescence of CD4+ and CD8+ T cells in these mice was enhanced by stimulation through CD3 and CD28. Each of phorbol 12-myristate 13-acetate (PMA) and ionomycin (IOM) stimulation weakly but their combined stimulation strongly enhanced EGFP expression. The stimulation-induced EGFP upregulation was also observed following T cell subset differentiation in a different manner. The EGFP induction by PMA + IOM stimulation was more potent than that by CD3/CD28 stimulation in helper T (Th)1, Th2, Th9, and regulatory T cells, while both stimulation conditions displayed the equivalent EGFP induction in Th17 cells. Our NFAT reporter mouse lines are useful for analyzing stimulation-induced transcriptional activation mediated by NFAT in cooperation with AP-1 in T cells.","PeriodicalId":12102,"journal":{"name":"Experimental Animals","volume":" ","pages":"454-459"},"PeriodicalIF":2.4,"publicationDate":"2023-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10658084/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9706937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0