European Journal of Dentistry最新文献

{"title":"Evaluation of Microstrain in the Regions Surrounding Morse Taper and External Hexagon Implants.","authors":"André Luiz de Melo Moreno, Marcio Campaner, Clóvis Lamartine de Moraes Melo Neto, Nathaly Vilene de Araújo Moreno, Daniela Micheline Dos Santos, Marcelo Coelho Goiato","doi":"10.1055/s-0044-1787696","DOIUrl":"10.1055/s-0044-1787696","url":null,"abstract":"<p><strong>Objective: </strong> The aim of this study was to compare the Morse taper (MT) + titanium base (Ti-Base) abutment with the external hexagon (EH) + Ti-Base abutment by using the strain gauge method in the mesial, distal, and apical-buccal areas around these types of implants.</p><p><strong>Materials and methods: </strong> This study investigated two groups, MT and EH, each comprising five polyurethane samples with a dental implant (3.75 × 11.5 mm) in the area of artificial tooth 15. The strain gauges were glued to the mesial, distal, and apical-buccal polyurethane areas of all samples in relation to the implant. Ti-Base nonangled abutments were installed on the implants in each group. Ten identical zirconia crowns were constructed by scanning and milling and were subsequently cemented onto the Ti-base abutments with calcium hydroxide cement. Then, an axial load of 100 N was applied to the occlusal region of the zirconia crowns, and strain gauge measurements were taken.</p><p><strong>Statistical analysis: </strong> Strain gauge data were assessed by a two-way analysis of variance (ANOVA) with \"implant connection\" and \"strain gauge position\" factors, followed by the Bonferroni test (<i>p</i> < 0.05).</p><p><strong>Results: </strong> The MT group showed significantly lower microstrain values in the mesial and apical strain gauges compared to the EH group.</p><p><strong>Conclusion: </strong> The MT group exhibited less microstrain in the mesial and apical areas of the polyurethane samples near the implant. Consequently, the MT connection was considered more biomechanically advantageous.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":"180-186"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11750305/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141626455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salivary Profile in Oral Submucous Fibrosis: A Scoping Review.","authors":"Fatma Yasmin Mahdani, Ajiravudh Subarnbhesaj, Nurina Febriyanti Ayuningtyas, Meircurius Dwi Condro Surboyo, Reiska Kumala Bakti, Desiana Radithia, Dimas Bayu Paramananda, Ina Indriyani, Fatimah Fauzi Basalamah","doi":"10.1055/s-0044-1788711","DOIUrl":"10.1055/s-0044-1788711","url":null,"abstract":"<p><p>Diagnosing oral submucous fibrosis (OSMF) is invariably challenging. The disease can be detected after reaching its final stage and requires complex treatment. Changes in its salivary profile can be used as a reference to see this disorder and as a basis for diagnostic prediction. This study is aimed to analyze the salivary profile as a diagnosis marker in patients with OSMF. The study using Preferred Reporting Items for Systematic Reviews and Meta-analyses was conducted using PubMed, Science Direct, and Scopus databases. A thorough literature search between 1991 and 2023 was performed. Twenty-eight full-text articles were reviewed in detail. Twenty-eight articles were included; a total of 929 patients of OSMF and 826 controls were found. The scoping review showed that levels of salivary protein (including lactate hydrogenase, immunoglobulin G, immunoglobulin A, S1007A protein, 8-hydroxydeoxyguanosine, 8-isoprostane, malondialdehyde, matrix metalloproteinase-12, salivary C-reactive protein, fibrinogen producing factor, salivary miRNA-21, and salivary lipids [cholesterol, high-density lipoprotein, triglyceride) were higher in OSMF. Meanwhile, trace elements (vitamin C, vitamin E, iron, zinc, and magnesium) were lower; only copper was higher in OSMF patients. Alteration in salivary components such as protein, lipid, and trace elements detection can be a basis for providing a noninvasive supportive examination and thus be used as a diagnosis marker of OSMF.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":"24-36"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11750339/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141893213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Finite Element Analysis of Anterior Implant-Supported Restorations with Different CAD-CAM Restorative Materials.","authors":"Usanee Puengpaiboon, Nichapat Rattanapan, Vamsi Krishna Pasam, Chamaiporn Sukjamsri","doi":"10.1055/s-0044-1785532","DOIUrl":"10.1055/s-0044-1785532","url":null,"abstract":"<p><strong>Objectives: </strong> Due to the lack of literature concerning the selection of crown materials for the restoration of anterior teeth, this study aimed to investigate the effects of six distinct computer-aided design and computer-aided manufacturing (CAD-CAM) crown materials on stress and strain distribution within implant-supported maxillary central incisor restorations, employing finite element analysis (FEA). Furthermore, a comparative analysis was conducted between models that incorporated adjacent natural teeth and those that did not, intending to guide the selection of the most suitable modeling approach.</p><p><strong>Materials and methods: </strong> Crown materials, including Lava Ultimate, Enamic, Emax CAD, Suprinity, Celtra Duo, and Cercon xt ML, were the subjects of the investigation. FEA models incorporating Coulomb friction were developed. These models were subjected to an oblique load, simulating the average maximum bite force experienced by anterior teeth. The potential for failure in titanium implant components and the prosthesis crown was evaluated through von Mises and principal stress, respectively. Furthermore, the failure of crestal bone was assessed through principal strain values.</p><p><strong>Statistical analysis: </strong> Stress values for each implant component and strain values of the bone were extracted from the models. To assess the impact of the six groups of crown materials, Kruskal-Wallis analysis of variance and post-hoc comparisons were conducted. Additionally, a statistical comparison between the two groups with Lava Ultimate and Cercon xt ML was performed using the Mann-Whitney U test to determine the difference in the two modeling approaches.</p><p><strong>Results: </strong> Higher crown material stiffness led to decreased stress in the abutment, fixture, and retaining screw, along with reduced strain in the surrounding bone. However, the decrease in stress and strain values became less significant with increasing crown stiffness. Additionally, the model with adjacent teeth showed significantly lower stress and strain concentrations compared to the model without adjacent teeth.</p><p><strong>Conclusion: </strong> Crowns with a high elastic modulus were the optimal choice for anterior teeth restoration. Constructing FEA models with adjacent teeth was highly recommended to gain a deeper understanding of the mechanical behavior of dental implant restorations.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":"37-47"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11750350/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140921536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Limosilactobacillus reuteri DSM 17938 Inhibition of Biofilm Formation by Prevotella intermedia and Fusobacterium nucleatum Across Salivary pH: An In Vitro Study.","authors":"Nissia Ananda, Vera Julia, Endang Winiati Bachtiar","doi":"10.1055/s-0044-1786846","DOIUrl":"10.1055/s-0044-1786846","url":null,"abstract":"<p><strong>Objectives: </strong> This study aims to investigate <i>Limosilactobacillus reuteri</i> DSM 17938's antibiofilm effects on <i>Prevotella intermedia</i> and <i>Fusobacterium nucleatum</i>, common causes of alveolar osteitis. It seeks topical alternatives to prevent this condition posttooth extraction. The secondary objective is to assess these effects under different pH conditions (pH 4.5 and pH 7), mimicking oral cavity saliva pH dynamics.</p><p><strong>Materials and methods: </strong> Ethical approval was secured for the saliva collection process involving five healthy adult participants who had undergone wisdom tooth extraction. Saliva samples were diligently collected on the 7th day post-surgery. The unstimulated saliva underwent a series of treatments, including the addition of phenylmethylsulfonyl fluoride (PMSF), pH adjustments, centrifugation, and filtration. The pH levels were re-measured, and subsequent adjustments were made to achieve pH values of 4.5 or 7. <i>Limosilactobacillus reuteri</i> DSM 17938, with a concentration of 1×10⁸ colony-forming units (CFU) per 5 drops, was utilized in the study. Biofilm testing involved incubating saliva samples with varying pH (4.5 or 7) alongside bacterial suspensions (<i>Prevotella intermedia, Fusobacterium nucleatum</i>, or a mixed species). The Interlac suspension was introduced, and plates were anaerobically incubated for 24 hours. Biofilm results were obtained using a spectrometer. The test is conducted in triplicate.</p><p><strong>Statistical analysis: </strong> To scrutinize the impact of pH on biofilm development, the acquired data underwent a two-way ANOVA test in SPSS as part of the statistical analysis. A significance level of <i>p</i><0.05 was used to determine statistical significance.</p><p><strong>Results: </strong> <i>Limosilactobacillus reuteri</i> DSM 17938 significantly reduced biofilm formation across bacterial strains (<i>p</i> = 0.000). Statistical analysis indicated a significant impact of pH on biofilm development (<i>p</i> = 0.000) compared to no saliva samples, with higher formation observed under acidic conditions (pH 4.5). However, the pH levels of 4.5 and 7 did not result in significantly different bacterial biofilm formation (<i>p</i> = 0.529).</p><p><strong>Conclusion: </strong> This research highlights <i>Limosilactobacillus reuteri</i> DSM 17938's potency in inhibiting biofilm formation of <i>Prevotella intermedia</i> and <i>Fusobacterium nucleatum</i>. Salivary pH variations significantly influence biofilm development, emphasizing the need to consider pH when assessing probiotic effectiveness. Despite limitations in saliva sample sterilization, this study provides valuable insights into alternative approaches for preventing alveolar osteitis. Further research should explore clinical applications and refine sterilization methods for more accurate results.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":"90-95"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11750330/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141467122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preosteoblast Adhesion and Viability Study of Freeze-Dried Bovine Bone Block Scaffold Coated with Human Umbilical Cord Mesenchymal Stem Cell Secretome.","authors":"Arum Nur Kartika Putri, David Buntoro Kamadjaja, Andra Rizqiawan, Muhammad Subhan Amir, Ni Putu Mira Sumarta, Dewi Kartikawati Paramita","doi":"10.1055/s-0044-1787105","DOIUrl":"10.1055/s-0044-1787105","url":null,"abstract":"<p><strong>Objectives: </strong> Combining a three-dimensional scaffold with growth factors before implantation is one method used to increase scaffold bioactivity in bone tissue engineering. The mesenchymal stem cell (MSC)-conditioned medium (CM), called secretome, contains many proteins and growth factors required for tissue repair and growth. This study evaluated the bioactivity of a bovine bone scaffold combined with the secretome of human umbilical cord MSCs (hUC-MSCs) by analyzing MC3T3-E1 cell adhesion and viability on the scaffold.</p><p><strong>Materials and methods: </strong> This <i>in vitro</i> laboratory study evaluated the effect of hUC-MSC secretome applied to bovine bone scaffolds processed using various techniques on MC3T3-E1 cell adhesion and viability. The three experimental groups included deproteinized bovine bone mineral-secretome (DBBM-CM), freeze-dried bovine bone-secretome (FDBB-CM), and decellularized FDBB-CM, whereas the control group was treated with DBBM alone. The cell adhesion test was performed using the centrifugation method after 6 and 24 hours, whereas the cell viability test was conducted using the trypan blue exclusion method after 24, 48, and 72 hours. Cell attachment was visualized after 4',6-diamidino-2-phenylindole staining and viewed under inverted fluorescence microscopy.</p><p><strong>Stastical analysis: </strong> Statistical analyses were performed using one-way analysis of variance, followed by a post hoc test in cases of significant differences.</p><p><strong>Results: </strong> Statistical analyses showed significantly greater adhesion of the preosteoblasts to the FDBB-CM scaffold at 6 hours (<i>p</i> = 0.002). The results of the adhesion test at 24 hours and the viability tests at all observation times showed no significant differences (<i>p</i> > 0.05). This study found that the average MC3T3-E1 cell adhesions and viabilities were highest for the FDBB-CM and DBBM-CM scaffolds. DBBM scaffolds with the secretome had better cell adhesion and viability than those without the secretome.</p><p><strong>Conclusion: </strong> The addition of MSC secretome increased bovine bone scaffold bioactivity especially in DBBM and FDBB scaffolds.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":"197-205"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11750312/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141626458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anticancer Potential of Quercetin on Oral Squamous Cell Carcinoma: A Scoping Review and Molecular Docking.","authors":"Dhona Afriza, Utmi Arma, Raefany Faslah, Wastuti Hidayati Suriyah","doi":"10.1055/s-0044-1789016","DOIUrl":"10.1055/s-0044-1789016","url":null,"abstract":"<p><p>Oral squamous cell carcinoma (OSCC) is a type of cancer that has a low survival rate and high recurrence and metastasis rates. To date, there is still no effective treatment for OSCC. Various types of cancer, including OSCC, have reported quercetin to act as an anticancer agent, but there is no clear research data on how it may affect OSCC. To determine the anticancer potential of quercetin in OSCC, we conducted a scoping review, and to determine the interaction of quercetin with one of the proteins that plays a role in carcinogenesis, namely, BCL-2, we conducted molecular docking. The scoping review process was conducted based on the Preferred Reporting Items for Systematic Reviews and Meta-Analyses Extension for Scoping Reviews. The scoping review was searched by collecting articles related to the research topic in Google Scholar, PubMed, ScienceDirect, Cochrane, and EBSCOhost databases. All of the literature records found during the search were imported into the Mendeley software to remove duplication. Nine studies were generated after the titles and abstracts were reviewed according to the inclusion and exclusion criteria. After the full-text screening, no studies were excluded, leaving nine publications determined to be eligible for inclusion in the scoping review. Quercetin showed effects on inhibiting cancer invasion, migration, proliferation, and many protein expressions, as well as increasing cell apoptosis. Molecular docking was done for quercetin and BCl-2 protein. Doxorubicin was utilized as a comparison ligand. The <i>in silico</i> study was utilized using AutoDock Vina, AutoDock Tools 1.5.6, Biovia Discovery Studio 2021, and PyMol. Molecular docking indicated quercetin has a strong binding affinity with BCl-2 protein (ΔG -7.2 kcal/mol). Both scoping review and molecular docking revealed that quercetin is a promising candidate for anticancer agent.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":"15-23"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11750326/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142344213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Successful Regenerative Endodontic Therapy of a Dens Evaginatus Mandibular Second Premolar with an Acute Apical Abscess and Extensive Periapical Bone Loss: A Case Report.","authors":"Francesco J DeMayo, Jackson T Seagroves, Takashi Komabayashi","doi":"10.1055/s-0044-1791218","DOIUrl":"10.1055/s-0044-1791218","url":null,"abstract":"<p><p>Regenerative endodontics is a rapidly growing field within dentistry that aims to replace damaged tooth structures and cells of the pulp-dentin complex. This case report presents the successful management of an immature permanent second mandibular premolar with an acute apical abscess and extensive apical bone loss caused by a dens evaginatus. This tooth was unexpectedly treated with long-term calcium hydroxide (Ca(OH)₂) that was replenished several times over 6 months. During the treatment process, this tooth became reinflamed developing a chronic apical abscess after resolution of the initial acute apical abscess, which resulted in the replacement and prolonged use of Ca(OH)₂. The final regenerative procedures were completed using a bioceramic fast set putty placed directly over the blood clot and an occlusal composite restoration. At the 6-month follow-up, the patient was asymptomatic with a fully healed radiographic lesion, new periodontal ligament, and the apex closed with thickening of the mesial and distal aspects of the root. At the 15-month follow-up, the patient remained asymptomatic with continued evidence of radiographic development of the apical aspect of this tooth, displaying root end closure and thickening. This is a unique case report of the management of a complex infection process in an immature tooth with regenerative techniques with repeated and long-term use of Ca(OH)₂. This novel report provides dental practitioners with a new potential protocol for the management of these immature cases with advanced periapical infections that require extensive disinfection to have successful outcomes.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":"255-264"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11750349/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142406271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histopathological Evaluation of Human Placental Extract as a Direct Pulp-Capping Material in Dogs' Teeth.","authors":"Rehab Khalil Safy, Mai Hamdy Ragab, Heba Bahgat Abdel-Maksoud","doi":"10.1055/s-0044-1786841","DOIUrl":"10.1055/s-0044-1786841","url":null,"abstract":"<p><strong>Objective: </strong> The current research aimed to evaluate the histopathological pulpal alterations in dogs' teeth following direct pulp capping using either mineral trioxide aggregate (MTA) or human placenta extract (HPE).</p><p><strong>Materials and methods: </strong> Forty-eight incisors with mature apices from four dogs were involved. The teeth were randomly allocated to three groups (<i>n</i> = 16) based on the material utilized for direct pulp capping: MTA, HPE, and Teflon as the negative control group. All involved teeth were capped and restored at the same session. Each group was subsequently divided into two subgroups (<i>n</i> = 8) based on the post treatment evaluation period: 2 and 4 weeks. The histopathologic changes in each specimen's pulp tissues, including pulp inflammation, hyperemia, necrosis, and dentin bridge development, were assessed. Then, all the data were statistically analyzed using chi-square, <i>t</i>-test, and one-way analysis of variance (<i>p</i> < 0.05).</p><p><strong>Results: </strong> At 2 weeks, chronic inflammation was observed in 100% of MTA and 50% of HPE subgroups with a significant difference between them whereas the remaining 50% exhibited no inflammation. In contrast to Teflon that showed acute inflammation, remission of inflammation was time-dependent at both MTA and HPE subgroups as there was a substantial difference between the 2- and 4-weeks evaluation periods within the same group. However, throughout the initially observed 2 weeks, all subgroups exhibited essentially no complete calcified bridge; at 4 weeks, all MTA and HPE subgroups developed dentin bridge formation, with a significant difference between them regarding its thickness.</p><p><strong>Conclusion: </strong> HPE is a promising pulp-capping material inducing less intense chronic inflammation accompanied with thicker dentine bridge formation in comparison to MTA.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":"144-153"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11750309/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141467121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and Characterization of Key Genes Associated with Amelogenesis.","authors":"Tahsinul Haque, Fatema Akhter, Nourelhoda Alim, Abdullah Nabhan, Fawzia Al Kahtani, Abdullah Mohammed Sambawa","doi":"10.1055/s-0044-1787958","DOIUrl":"10.1055/s-0044-1787958","url":null,"abstract":"<p><strong>Objectives: </strong> The identification of key genes associated with amelogenesis would be helpful in finding solutions to genetic disorders in oral biology. The study aimed to use <i>in silico</i> analysis to identify the key genes involved in tooth development associated with preameloblasts (PABs) and secretory ameloblasts (SABs).</p><p><strong>Material and methods: </strong> The data was subjected to quality analysis and uniform manifold approximation and projection analysis. To examine the distribution of the genes and identify important upregulated loci, a <i>p</i>-value histogram, a quantile plot, a mean difference and mean-variance plot, and a volcano plot were generated. Finally, protein-protein interaction and gene enrichment analyses were performed to determine the ontology, relevant biological processes, and molecular functions of selected genes.</p><p><strong>Results: </strong> A total of 157 genes were found to be significant in the PAB versus SAB comparison. HIST1H31 revealed strong interaction with HIST1H2BM, and EXO1, ASPM, SPC25, and TTK showed strong interactions with one other. The STRING database revealed that NCAPG, CENPU, NUSAP1, HIST1H2BM, and HIST1H31 are involved in biological processes. NCAPG, CENPU, SPC25, ETV5, TTK, ETV1, FAM9A, NUSAP1, HIST1H2BM, and HIST1H31 are involved in cellular components.</p><p><strong>Conclusion: </strong> The TTK, NUSAP1, CENPU, NCAPG, FAM9A, ASPM, SPC25, and HIST1H31 genes demonstrate functions in cell division. These genes might play a role in ameloblast development. These results will be useful in developing new methods to stimulate ameloblast development, which is essential for tooth regeneration and tissue engineering. However, more research is required to validate the functions of these genes and the genes with which they interact. A wide variety of genetic, epigenetic, and exogenous signaling factors regulate these genes and pathways throughout development and differentiation, cell fate, and behavior.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":"116-123"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11750345/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142282559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Vertical Force Generated with Single File Systems during Shaping of Constricted Root Canals.","authors":"Abdulmohsen Alfadley, Ahmed Jamleh","doi":"10.1055/s-0044-1786840","DOIUrl":"10.1055/s-0044-1786840","url":null,"abstract":"<p><strong>Objectives: </strong> This study aimed to evaluate the shaping force generated with OneShape (OS) and HyFlex EDM (HEDM) systems designed for single file shaping, in comparison with ProTaper Next (PTN).</p><p><strong>Materials and methods: </strong> Maxillary premolar teeth received access cavity preparation and their canals were shaped with OS, HEDM, or PTN to size 25 according to manufacturer's instructions with consistent pressure on the files to give a gentle \"in-and-out\" movements of 2 mm amplitude. The canal shaping was completed with a total of three insertions. After each insertion, 1% NaOCl irrigation and recapitulation with K-file size 15 were performed. The vertical shaping force was measured using a force gauge (M5-20 Advanced Digital Force Gauge; Mark-10 Corporation, NY).</p><p><strong>Statistical analysis: </strong> The shaping time was analyzed by using a one-way analysis of variance (ANOVA) test and differences between the mean apical and coronal maximum force values were analyzed using the Kruskal-Wallis and Mann-Whitney U tests. The level of significance was set as 0.05.</p><p><strong>Results: </strong> The magnitude of the vertical forces increased with successive advancements of the instruments within the canal. During canal shaping procedures in all groups, the apical and coronal maximum force values of the OS and HEDM ranged from 2.5 to 7.2 N and 1.3 to 2.9 N, respectively. PTN generated the lowest maximum apical forces during the second and third insertions (<i>p</i> < 0.05). HEDM generated significantly less maximum coronal forces than both OS and PTN during the first insertion while the use of OS was associated with the highest amount of force values in the second and third insertions (<i>p</i> < 0.05). In terms of shaping time, no significant differences were detected among the three tested systems (<i>p</i> = 0.606).</p><p><strong>Conclusion: </strong> The tested single file systems were associated with higher shaping forces in the apical direction that were significant in the second and third insertions.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":"110-115"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11750318/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141626425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}