European Journal of Dentistry最新文献

{"title":"Comparative Analysis of CAD-CAM Workflow Variations on the Marginal and Internal Gaps and Fatigue Behavior of Ceramic and Resin Composite Dental Crowns.","authors":"Rafaela Oliveira Pilecco, Lucas Saldanha da Rosa, Andrea Baldi, Renan Vaz Machry, João Paulo Mendes Tribst, Luiz Felipe Valandro, Cornelis Johannes Kleverlaan, Nicola Scotti, Gabriel Kalil Rocha Pereira","doi":"10.1055/s-0044-1791705","DOIUrl":"10.1055/s-0044-1791705","url":null,"abstract":"<p><strong>Objectives: </strong> To analyze the marginal/internal gap and the fatigue behavior of crowns made of two different materials, using four combinations of a digital workflow-two intraoral scanners (IOSs) and two milling machines.</p><p><strong>Materials and methods: </strong> Crowns were made considering three factors: IOS (a confocal microscopy-based scanner: TRIOS 3-TR; or a combination of active triangulation and dynamic confocal microscopy: Primescan-PS), milling machines (four-axis: CEREC MC XL-CR or five-axis: PrograMill PM7-PM), and restorative material (lithium disilicate-LD or resin composite-RC) (<i>n</i> = 10). The bonding surface of each crown was treated and bonded to each respective glass fiber-reinforced epoxy resin die using a dual-cure resin cement. A computed microtomography analysis was performed to access marginal/internal gap. The specimens were subjected to a cyclic fatigue test (20 Hz, initial load = 100 N/5,000 cycles; step size= 50 N/10,000 cycles until 1,500 N, then if specimens survived, the step size was increased to 100 N/10,000 cycles).</p><p><strong>Statistical analysis: </strong> For data analysis, three-way analysis of variance and Kaplan-Meier with log-rank (Mantel-Cox) test were performed (α = 0.05).</p><p><strong>Results: </strong> TR resulted in a smaller axial-occlusal angle and occlusal gap, and five-axis milling resulted in a smaller marginal gap, axial-occlusal angle, and occlusal gap. Angled points and occlusal surface showed a tendency for overmilling. RC crowns displayed higher survival rates and a more pronounced topography compared with LD independently of the scanning and milling method. LD crowns produced with a five-axis milling machine resulted in higher fatigue performance and rougher topography compared with a four-axis machine.</p><p><strong>Conclusion: </strong> RC crowns displayed better fatigue behavior compared with LD, while LD benefited from a five-axis machine for improved survival probability.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":"638-649"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12182427/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143614117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Silanized Nanographene Oxide Concentrations in Different Primers on Bonding Durability between Resin Cement and Zirconia.","authors":"Ahmed Q Mahmoud, Tarek Ahmed Soliman, Tarek A Elkhooly, Asmaa Harhash, El-Sayed Gad Eid","doi":"10.1055/s-0044-1795126","DOIUrl":"10.1055/s-0044-1795126","url":null,"abstract":"<p><strong>Objectives: </strong> Zirconia (ZrO₂) has been used in dental restorations due to its increased mechanical properties, biocompatibility, low degree of bacterial adhesion, and acceptable optical properties. One of the major drawbacks of ZrO₂ is its short-term durable bond with resin cement. The objective of this study was to evaluate the effect of different primers embedded with silanized nanographene oxide (SGO) sheets on the wettability of ZrO₂ surface and bond strength durability between resin cement and ZrO₂.</p><p><strong>Materials and methods: </strong> Four hundred ZrO₂ specimens were divided into four main groups as each group had 100 specimens according to the type of the primer: rely X ceramic primer (Group I), monobond N primer (Group II), monobond plus primer (Group III), and Z prime plus primer (ZP, Group IV). Each main group was subdivided into five subgroups according to SGO concentrations by weight blended into primers: (1) 0% (control), (2) 0.1%, (3) 0.3%, (4) 0.6%, and (5) 0.9% as each subgroup had 20 specimens. Immediate shear bond strength (SBS) test was done for half of the specimens per each subgroup (10 specimens) by universal testing machine, the other half of the specimens per each subgroup (10 specimens) were exposed to thermocycling for 10,000 cycles that is equivalent to 1 year of clinical use at controlled temperatures (5-55°C) by thermocycler then SBS test by universal testing machine was done. Water contact angle test was done for all specimens per each subgroup (20 specimens) by computer software and an optical tensiometer.</p><p><strong>Results: </strong> The SBS was nonsignificantly decreased after thermocycling for all primers embedded with SGO except for ZP primer. The best wettability of ZrO₂ surface was found in (ZP) primer group embedded with (0.9% SGO) with a mean value of 20.60.</p><p><strong>Conclusion: </strong> Primers embedded with SGO could increase the wettability of the ZrO₂ surface and bond strength durability between resin cement and ZrO₂ even after thermocycling aging. The clinical significance of this study was the possible increase of the wettability of ZrO₂ surface and SBS of resin cement to ZrO₂ with promising long-term stability when commercial primers embedded with SGO were used. This could reduce the risk of debonding between resin cement and ZrO₂ crowns or veneers.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":"801-810"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12182426/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143596334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histone H3K9 Methylation Is Differentially Modified in Odontogenic Cyst and Tumors.","authors":"Ekarat Phattarataratip, Aroonwan Lam-Ubol","doi":"10.1055/s-0044-1791681","DOIUrl":"10.1055/s-0044-1791681","url":null,"abstract":"<p><strong>Objectives: </strong> Histone modification in odontogenic lesions is mostly unexplored. Trimethylation of histone H3 at lysine residue 9 (H3K9Me3) has been studied in various pathologic conditions and showed biological significance promising for future therapeutic application. This study aimed to investigate the level and clinical relevance of the H3K9Me3 histone modification in odontogenic cysts and tumors.</p><p><strong>Materials and methods: </strong> A total of 105 cases of odontogenic lesions, comprising 30 odontogenic keratocysts (OKCs), 30 adenomatoid odontogenic tumors (AOTs), 30 ameloblastomas, and 15 dental follicles, were included in the study. The paraffin-embedded tissues were immunohistochemically stained for H3K9Me3. Both the intensity and the distribution of staining were evaluated and calculated as H-score. The correlation between the H3K9Me3 expression and the clinical characteristics of each lesion was evaluated.</p><p><strong>Statistical analysis: </strong> The Kruskal-Wallis test followed by Bonferroni's correction was performed to assess the differences in H-score among groups. In addition, Pearson's chi-squared test or Mann-Whitney <i>U</i> test was used to analyze potential factors that could affect protein expression.</p><p><strong>Results: </strong> The reduced enamel epithelium of the dental follicle showed uniformly strong H3K9Me3 expression. All odontogenic cysts and tumors examined demonstrated a significantly reduced H3K9Me3 level compared with dental follicles. The AOT showed the lowest H3K9Me3 level, followed by OKC and ameloblastoma. Its immunoreactivity was mainly localized in the basal and parabasal cells of OKC and the whorled/duct-like structures of AOT. Ameloblastoma exhibited marked variation in the H3K9Me3 level among cases. Notably, the upregulated H3K9Me3 was related to multilocularity of OKC and ameloblastoma.</p><p><strong>Conclusion: </strong> Histone H3K9 methylation is differentially expressed in odontogenic cysts and tumors. This epigenetic modification may contribute to the pathogenesis and aggressive behavior of odontogenic lesions.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":"688-696"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12182423/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142603751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-Inflammatory Effects of Calcium Hydroxide Combined with Ellagic Acid as Pulp Capping Material: In Vivo Study.","authors":"Annisa Fitria Sari, Intan Nirwana, Anita Yuliati, Asti Meizarini, Retno Pudji Rahayu, Retno Palupi, Michelle Fidelia Alexandra, Tarissa Balqis Nuraida, Meircurius Dwi Condro Surboyo, Khairul Anuar Shariff","doi":"10.1055/s-0044-1791243","DOIUrl":"10.1055/s-0044-1791243","url":null,"abstract":"<p><strong>Objectives: </strong> Pulp capping is a pivotal treatment in dentistry aimed at preserving pulp vitality. While calcium hydroxide has long been considered the gold standard in pulp capping materials, its long-term use can induce chronic inflammation, ultimately leading to pulp necrosis and affecting human health. In this context, ellagic acid, a natural compound with potent anti-inflammatory properties, emerged as a promising adjunct to mitigate inflammation associated with calcium hydroxide application, thereby sustaining pulp vitality. This study aimed to investigate the inflammatory response by alterations in neutrophil, macrophage, lymphocyte, and tumor necrosis factor-α (TNF-α) expression following the treatment with a combination of calcium hydroxide and ellagic acid.</p><p><strong>Materials and methods: </strong> Dental pulp perforation was made on 27 male Wistar rats on the upper first molar and then pulp capped with calcium hydroxide and ellagic acid. The pulp of the control positive group was capped with calcium hydroxide, and the control group was not capped. The teeth were then extracted after 1, 3, and 7 days posttreatment. The differences in the number of neutrophils, macrophages, lymphocytes, and TNF-α expression were analyzed using one-way analysis of variance (ANOVA) and Tukey's honestly significant difference (HSD) test.</p><p><strong>Results: </strong>The treatment combination of calcium hydroxide and ellagic acid showed the lowest neutrophil number and TNF-α expression compared with the other groups (<i>p</i> < 0.05), while the macrophage and lymphocyte numbers were the highest compared with the other groups (<i>p</i> < 0.05).</p><p><strong>Conclusion: </strong> The combination of calcium hydroxide and ellagic acid as a pulp capping material exhibited a dual effect on the inflammatory response in dental pulp. These findings suggest that calcium hydroxide and ellagic acid modulate the inflammatory response in a complex manner, promoting a more controlled and potentially beneficial healing process.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":"624-629"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12182428/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142603742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biodegradation, Angiogenesis, and Inflammatory Response of a Collagen-Chitosan-Polyvinyl Alcohol (PVA) Membrane: In Vivo Model of Guided Tissue Regeneration.","authors":"Ira Komara, Furi Andanawari, Agus Susanto, Euis Reni Yuslianti, Ina Hendiani, Prajna Metta, Amaliya Amaliya","doi":"10.1055/s-0044-1801305","DOIUrl":"10.1055/s-0044-1801305","url":null,"abstract":"<p><p>The aim of this study was to examine the biodegradation, angiogenesis, and inflammatory response in collagen-chitosan-polyvinyl alcohol (PVA) membranes.This study employed an experimental approach utilizing a randomized controlled trial design. Wistar rats were used as subjects, with 51 rats divided into three groups. Each group received a different treatment: application of the collagen-chitosan-PVA membrane, pericardial membrane, or cross-linked pericardial membrane, administered subcutaneously. On days 0, 7, 14, and 30, the rats were terminated, and the membranes and surrounding tissues were collected for analysis. A histological examination was performed to evaluate the membrane biodegradation rate, the number of blood vessels formed, and the inflammatory response.The data were analyzed using the Kruskal-Wallis and Mann-Whitney tests, with a <i>p</i>-value of < 0.05 considered statistically significant.The collagen-chitosan-PVA membrane remained in the tissue up to day 30, while the pericardial membrane and cross-linked pericardial membrane were completely degraded by day 7. The average number of new blood vessels formed in the collagen-chitosan-PVA membrane on days 7, 14, and 30 was greater than that in the pericardial membrane and cross-linked pericardial membrane, which was statistically significant (<i>p</i> < 0.005). The average number of inflammatory cells in the collagen-chitosan-PVA membrane on day 30 was lower than that in the pericardial membrane and cross-linked pericardial membrane, which was statistically significant (<i>p</i> < 0.005) for neutrophils, monocytes, and lymphocytes. However, the difference was not statistically significant (<i>p</i> > 0.05) for eosinophils and mast cells.Biodegradation, angiogenesis, and the inflammatory response in collagen-chitosan-PVA membranes showed better results compared with other membranes. Collagen-chitosan-PVA membranes exhibit potential for application in guided tissue regeneration treatment for periodontal disease.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":"835-842"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12182392/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143960529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Characteristic of Transverse Dental Arch Relationship Study in Unilateral Cleft and Palate versus Noncleft in Thai Northeastern Population.","authors":"Nattharin Wongsirichat, Araya Pisek, Aggasit Manosudprasit","doi":"10.1055/s-0044-1795121","DOIUrl":"10.1055/s-0044-1795121","url":null,"abstract":"<p><strong>Objectives: </strong> To evaluate and compare the transverse dental arch relationship of patients with unilateral cleft lip and palate (UCLP) versus the noncleft controls in Northeastern region of Thailand.</p><p><strong>Materials and methods: </strong> A cross-sectional study involving 80 participants comprising 40 nonsyndromic children with UCLP and 40 healthy noncleft children of similar age (mean age: 11 years). The study was conducted at the Faculty of Dentistry, Khon Kaen University, Thailand. Each participant underwent assessments for Angle's classification, overjet, overbite, and transverse dental arch width, along with the evaluation of the modified Huddart/Bodenham (MHB) index using three-dimensional digital dental casts. Comparisons between groups were performed using paired sample <i>t</i>-tests and nonparametric Mann-Whitney tests, with a significance level established at <i>p</i> < 0.05.</p><p><strong>Results: </strong> The sample was categorized into two groups: the early mixed dentition group (ages 7-10 years) and the late mixed dentition group (ages 11-14 years). In the antero-posterior plane, individuals with UCLP and those without cleft presented with class II Angle's classification. In the transverse plane, the maxillary transverse arch width was notably narrower in UCLP cases compared with noncleft cases, with more pronounced differences observed in the late mixed dentition group. However, there was no statistically significant difference in mandibular transverse width between the two groups. The mean MHB index scores were -9.35 in the early mixed dentition group and -12.63 in the late mixed dentition group, indicating a more severe score in the latter. When compared with the noncleft control group, both UCLP groups showed significantly lower MHB index scores.</p><p><strong>Conclusion: </strong> In comparison to noncleft individuals, the majority of UCLP cases exhibited class II angles with negative overjet. A significantly smaller transverse arch width was observed in the maxilla of UCLP patients, with no significant variances noted in the mandible. Analysis using the MHB index indicated greater total arch constriction in UCLP cases, particularly in the anterior tooth region. Furthermore, the severity of these findings was observed to escalate with age.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":"758-767"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12182398/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143986862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improvement of Dentin Growth Parameters (Beta-catenin, bFGF, CD105, and BMP4) with Propolis as Adjuvant in Dental Caries Treatment.","authors":"Retno Pudji Rahayu, Nirawati Pribadi, Ira Widjiastuti, Nur Ariska Nugrahani","doi":"10.1055/s-0044-1791939","DOIUrl":"10.1055/s-0044-1791939","url":null,"abstract":"<p><strong>Objective: </strong> The purpose of the study was to evaluate the efficacy of calcium hydroxide (Ca(OH)₂) and propolis in pulp capping for dental caries treatment, focusing on dentin growth parameters. The study also aims to determine the role of propolis as a natural adjuvant therapy in enhancing reparative dentin development while emphasizing the importance of proper technique and material preparation with markers for the expression of beta-catenin, bFGF, CD105, and BMP4.</p><p><strong>Materials and methods: </strong> The left bottom molar teeth from 28 Wistar rats were divided into four groups. The first group, the control group, was given only aqua dest, and the second group received drilling treatment and additional therapies with Ca(OH)₂ (Ca(OH)₂) 0.625 μg. The third group was given drilling treatment and additional therapies with a combination of propolis with Ca(OH)₂ 0.781 μg until day 7. Finally, the fourth group received a combination of propolis with Ca(OH)₂ 0.781 μg until day 14. This research analyzed the expression of essential basic fibroblast growth factor (bFGF), CD105, beta-catenin, and bone morphogenetic protein 4 (BMP4).</p><p><strong>Results: </strong> This research reports that the average expression of BMP4 and bFGF showed a significant result in treatment with additional therapies with propolis and Ca(OH)₂. The experiment indicates that propolis and Ca(OH)₂ could induce reparative dentine on days 7 and 14.</p><p><strong>Conclusion: </strong> Propolis as an adjuvant shows better reparative dental formation with improvement in the expression of bFGF and BMP4 in 14 days of therapy.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":"729-734"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12182417/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142806569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of Vertical Marginal Discrepancy in High and Low Translucent Monolithic Zirconia Crowns in Repeated Firing Cycles.","authors":"Mehrzad Moazzam, Amirhossein Fathi, Mahsa Ghorbani, Ramin Mosharraf","doi":"10.1055/s-0044-1801303","DOIUrl":"10.1055/s-0044-1801303","url":null,"abstract":"<p><strong>Objective: </strong> Increase in vertical marginal discrepancy (VMD) during repeated firing cycles and its clinical outcomes is a major concern for high and low translucent monolithic zirconia crowns. The purpose of this in vitro study was to evaluate and compare VMD in high and low translucent monolithic zirconia crowns in repeated firing cycles.</p><p><strong>Material and methods: </strong> To perform this study, 10 monolithic zirconia crowns made by computer-aided design and computer-aided manufacturing method were used in two groups of five with high and low translucency, which were designed on Zimmer tissue-level implant abutment. The crowns in each group were randomly numbered from 1 to 5 and each underwent 1, 3, and 5 firing cycles. After completing each mentioned cycle, the VMD was measured at eight predetermined points on abutment by optical microscope and their average was recorded for each sample. Data analysis was done by SPSS 22 software through repeated-measure analysis of variance, paired <i>t</i>-test, and <i>t</i>-test with a 5% significance threshold.</p><p><strong>Results: </strong> A total of 240 measurements were made for the VMD, which, due to the presence of five samples in each translucency group and eight examined points in each sample, was finally summed up to six averages for each translucency group in the mentioned three stages of firing cycles. The averages for the low-translucency group after 1, 3, and 5 firing cycles were 76.86, 85.02, and 90.55 μm, respectively, and for the high-translucency group after 1, 3, and 5 firing cycles were 80.38, 87.33, and 97.78 μm, respectively. The average VMD of the samples regardless of the translucency level after 1, 3 and 5 firing cycles was calculated as 78.62, 86.18, and 94.16 μm, respectively.</p><p><strong>Conclusion: </strong> This study found that VMD increased with repeated firing cycles, with no significant difference between high- and low-translucency zirconia crowns. Repeated firings significantly raised VMD, but all values remained within clinically acceptable limits, supporting the suitability of both translucency types for clinical use.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":"843-850"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12182429/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143614125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fracture Resistance of Resin Matrix Ceramic Post and Core Compared to Prefabricated Fiber-Reinforced Composite Post and Core in Non-Ferrule Effect Teeth: An In Vitro Study.","authors":"Keeratikarn Kunawongkrit, Basel Mahardawi, Pheeradej Na Nan, Palawat Laoharungpisit, Kwanchanok Ratanakupt, Napapa Aimjirakul","doi":"10.1055/s-0044-1789001","DOIUrl":"10.1055/s-0044-1789001","url":null,"abstract":"<p><p>This research studies the fracture resistance of a non-ferrule endodontically treated tooth restored with two types of resin matrix ceramic (Enamic and Cerasmart) post and core compared with the conventional prefabricated fiber-reinforced composite (FRC) post and core.Thirty single-root-canal premolars were sectioned to 13 mm root length and then all the roots were filled, using a crown-down technique for root canal preparation and one cone technique for root canal obturation, All the roots were randomly divided into three groups (<i>n</i> = 10) according to post and core material; (1) Enamic group (EN), (2) Cerasmart group (CM), and (3) prefabricated fiber post group (FRC). Three groups of specimens were tested using a universal testing machine (EZ Test Series, Shimadzu, Japan). Specimen blocks were fixed into a holder with an inclination of 45 degrees. The force was loaded to a palatal incline of buccal cusp at a crosshead speed of 0.5 mm/minute until there was split or fracture of the tooth. The maximum braking force was recorded in Newton (N), and the mode of failure was observed by a stereomicroscope.The dependent variable was fracture resistance (maximum breaking force) and the data were analyzed using one-way analysis of variance and then multiple comparison Tukey's tests were used, aiming to find means that are significantly different among the groups. Moreover, the failure mode was analyzed using the chi-square test.A significant difference was recorded. Teeth restored with prefabricated FRC had the most fracture resistance 342.19 ± 79.34 N (<i>p</i> = 0.007), followed by the resin matrix ceramic group (265.10 ± 48.58 N: Cerasmart) and Enamic group (260.98 ± 43.96 N: Enamic). No significant difference between the Enamic and Cerasmart groups was noted. Additionally, no significant difference in the mode of failure was recorded among the three groups.The fracture resistance of a non-ferrule endodontically treated tooth restored with the conventional prefabricated FRC post and core is greater than that restored with either type of resin matrix ceramic (Enamic and Cerasmart). This explains the higher failure rate resulting from the use of resin matrix ceramics.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":"630-637"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12182421/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143623980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocompatibility and Bioactivity Evaluation of Novel Calcium Silicate-Based Sealer: In Vitro Study on Human Dental Pulp Stem Cells.","authors":"Anggraini Margono, Redho Sara Pratiwi, Anggita Dini Nofarina, Dewa Ayu Npa, Ike Dwi Maharti, Romilda Rosseti","doi":"10.1055/s-0045-1802566","DOIUrl":"10.1055/s-0045-1802566","url":null,"abstract":"<p><strong>Objective: </strong> This article evaluates the biocompatibility and bioactivity of a novel calcium silicate-based sealer by assessing its impact on the viability, mineral deposition, wound closure, and migration activity of human dental pulp stem cells (hDPSCs).</p><p><strong>Material and methods: </strong> AH Plus and AH Plus Bioceramic were pulverized and sterilized according to International Organization for Standardization 10993-5:2009. The hDPSCs were stored raw materials, reaching 80% confluence after passing stem cell marker tests (CD90 98%, CD105 99.7%, CD73 94%, and LinNeg 0.5%) and were at passage 5 to 6 after serum starvation for 24 hours. The study consisted of four groups: AH Plus at concentrations of 1:1 and 1:4, and AH Plus Bioceramic at concentrations of 1:1 and 1:4. Viability was assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay by measuring optical density values, while mineral deposition was evaluated through Alizarin red staining and analyzed with ImageJ software. Migration activity was measured by calculating migration speed and wound closure percentage using a scratch assay at 24 and 72 hours, with results analyzed by ImageJ.</p><p><strong>Statistical analysis: </strong> Viability, migration, and wound closure results were analyzed using one-way analysis of variance. Mineral deposition was analyzed descriptively.</p><p><strong>Results: </strong> There were significant differences in the viability and mineral deposition of hDPSCs between calcium silicate-based sealers and epoxy resin-based sealers. Calcium silicate-based sealers showed higher viability and better mineral deposition. The migration speed rate of hDPSCs at 24 hours and wound closure at 24 and 72 hours were significantly greater with the novel calcium silicate-based sealer compared with the epoxy resin-based sealer.</p><p><strong>Conclusion: </strong> This study suggests that calcium silicate-based sealers offer advantages over traditional epoxy resin-based sealers, demonstrating superior biocompatibility and bioactivity. These properties may lead to improved clinical outcomes, such as faster healing and fewer posttreatment complications. Further research is needed to explore the full potential of these materials in endodontics.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":"777-783"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12182422/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143977562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}