Abdul Samad Khan, Ahlam AlAbdali, Nadia Irshad, Othoob AlMusayyab, Norah AlQahtani, Asma Tufail Shah, Sultan Akhtar, Yassine Slimani
{"title":"Evaluation of Mechanical and Elemental Properties of Bioceramic-Coated Orthodontic Brackets and Enamel Surface.","authors":"Abdul Samad Khan, Ahlam AlAbdali, Nadia Irshad, Othoob AlMusayyab, Norah AlQahtani, Asma Tufail Shah, Sultan Akhtar, Yassine Slimani","doi":"10.1055/s-0044-1789003","DOIUrl":"https://doi.org/10.1055/s-0044-1789003","url":null,"abstract":"<p><strong>Objective: </strong> The aim is to coat orthodontic brackets with two different bioactive materials and to compare the mechanical and morphological properties of coated brackets and tooth surfaces.</p><p><strong>Materials and methods: </strong> A total of 120 stainless steel brackets were divided equally into three groups, that is, the uncoated brackets and nanohydroxyapatite (nHA)-coated, and nanobioactive glass (nBG)-coated brackets using a spin coater machine. The brackets were bonded on the enamel surface and underwent remineralization/demineralization cycles for days 1, 7, 14, and 30. At each time interval, the bond strength of the brackets was assessed using mechanical loading. An optical and scanning electron microscope (SEM) were used for surface evaluation, and the adhesive remanent index (ARI) values were obtained and quantified.</p><p><strong>Statistical analysis: </strong> One-way analysis of variance using Tukey's test was used to compare the differences among the groups.</p><p><strong>Results: </strong> A uniform distribution of nanoparticles occurred on the surfaces of brackets. The shear bond strength (SBS) showed no significant differences in any tested groups on days 1, 7, and 14. However, control and nBG showed a significant difference from nHA at day 30. On days 7, 14, and 30, the nHA group showed the highest SBS values among the groups. For ARI, most samples showed an adhesive nature of failure at the enamel-brackets interface. The images confirmed the presence of coated particles on brackets and remnants of adhesives after SBS.</p><p><strong>Conclusion: </strong> This study confirmed that the nHA- and nBG-coated brackets have a high potential for application in orthodontics regarding structural and mechanical properties.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142282558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comparison of Water Sorption and Water Solubility Properties of Current Restorative Materials with Different Contents.","authors":"Magrur Kazak, Tugba Toz Akalin, Fevzi Esen","doi":"10.1055/s-0044-1789270","DOIUrl":"https://doi.org/10.1055/s-0044-1789270","url":null,"abstract":"<p><strong>Objectives: </strong> This study aimed to investigate and compare water sorption and solubility properties of current restorative materials with different contents.</p><p><strong>Materials and methods: </strong> Alkasite, self-adhesive restorative material (Cention N, Ivoclar Vivadent AG, Schaan, Liechtenstein), bulk-fill glass hybrid restorative material (EQUIA Forte HT, GC Corp., Tokyo, Japan), nanohybrid universal composite material (OptiShade, Kerr Dental, United States), and bulk-fill composite material (Filtek One Bulk Fill Restorative, 3M ESPE, St. Paul, Minnesota, United States) were used. Samples (<i>n =</i> 6) were prepared (2 × 10 mm) according to the ISO 4049 standards. Water sorption and solubility values were calculated according to the ISO 4049 standards.</p><p><strong>Statistical analysis: </strong> One-way ANOVA, Tukey's post-hoc, Tamhane's T2 post-hoc, Pearson's correlation, and independent samples <i>t</i>-tests were used for statistical analysis (<i>p <</i> 0.05).</p><p><strong>Results: </strong> Group EQUIA Forte HT significantly showed the highest water sorption values (57.278 ± 3.174), while Group Filtek One Bulk Fill Restorative exhibited the lowest (4.429 ± 0.174; <i>p <</i> 0.05). The water sorption values for Group Cention N were 5.000 ± 0.542. Group EQUIA Forte HT significantly had the lowest water solubility values (-99.799 ± 1.909), while Group Cention N (-2.966 ± 0.402) significantly exhibited the highest (<i>p <</i> 0.05). There was no significant correlation between water sorption and solubility values for each material (<i>p ></i> 0.05).</p><p><strong>Conclusion: </strong> The bulk-fill nano-filled composite resin material was successful in terms of water sorption while the bulk-fill glass hybrid restorative system in terms of water solubility. Alkasite can be recommended to be used as a base material due to its high solubility feature. Monomer, filler type, and amount had an impact on the water sorption and solubility properties of the tested materials.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142282542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ike Dwi Maharti, Romilda Rosseti, Dini Asrianti, Nendar Herdianto, Winda Rianti
{"title":"Calcium Silicate-Based Sealers: Apatite Deposition on Root Canal Dentin and pH Variation Analysis.","authors":"Ike Dwi Maharti, Romilda Rosseti, Dini Asrianti, Nendar Herdianto, Winda Rianti","doi":"10.1055/s-0044-1788685","DOIUrl":"https://doi.org/10.1055/s-0044-1788685","url":null,"abstract":"<p><strong>Objective: </strong> This study analyzes the biomineralization potential of calcium silicate-based sealers Ceraseal (Meta Biomed Co., Cheongju, Korea) and AH Plus Bioceramic (Dentsply Sirona, United States), focusing on evaluating apatite deposition in root canal dentin and pH increases.</p><p><strong>Materials and methods: </strong> Calcium silicate-based sealers Ceraseal (Meta Biomed Co.) and AH Plus Bioceramic (Dentsply Sirona) were applied to the root canal dentin of premolars that had undergone root canal preparation procedures. This was followed by a 14-day immersion in phosphate-buffered saline (PBS). Biomineralization analysis was performed by analyzing the formation of the apatite layer after the 14-day immersion. The thickness of the apatite layer deposits was observed using a scanning electron microscope (SEM). Additionally, the sealers were placed in molds and submerged in PBS solution with pH measurements taken on days 0, 7, and 14 using a digital pH meter.</p><p><strong>Statistical analysis: </strong> The average thickness of apatite deposition in the interfacial layer was analyzed using the Mann-Whitney's test. The pH value differences among the groups were analyzed using a one-way analysis of variance test, followed by a post hoc least significant difference.</p><p><strong>Results: </strong> There were differences in the apatite deposition in the interfacial layer between Ceraseal and AH Plus Bioceramic within 14 days of observation. There was a significant difference (<i>p</i> < 0.05) between the pH values of Ceraseal and AH Plus Bioceramic at 7 and 14 days of observation. Ceraseal showed greater alkalizing activity compared with AH Plus Bioceramic.</p><p><strong>Conclusion: </strong> Calcium silicate-based sealer Ceraseal showed better biomineralization potential than AH Plus Bioceramic.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142282541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Evaluating the Efficacy of Resveratrol-Containing Mouthwash as an Adjunct Treatment for Periodontitis: A Randomized Clinical Trial.","authors":"Sura A Mohammed, Hadeel Mazin Akram","doi":"10.1055/s-0044-1788686","DOIUrl":"https://doi.org/10.1055/s-0044-1788686","url":null,"abstract":"<p><strong>Objectives: </strong> To evaluate the effectiveness of resveratrol mouthwash as an adjunct to nonsurgical periodontal treatment of periodontitis.</p><p><strong>Materials and methods: </strong> This study was a randomized, double-blind clinical trial study. The study included 57 participants with periodontitis. Clinical parameters (plaque index [PI], bleeding on probing [BOP], probing pocket depth [PPD], and clinical attachment loss [CAL]) were examined at the baseline visit, after 7 days, and after 30 days of using resveratrol mouthwash as an adjunct to nonsurgical periodontal treatment. The salivary levels of (interleukin [IL]-6) and RANKL (receptor activator of nuclear factor-kappa B ligand) were measured and compared before and after treatment. The participants answered the visual analog scale-based assessment questionnaire at the last visit.</p><p><strong>Statistical analysis: </strong> A one-way ANOVA (analysis of variance) test was used to compare the means of multiple groups (test, positive control, negative control) at baseline and after treatment. A paired <i>t</i>-test was also used to compare the means of a single group before and after treatment. In addition, Tukey's multiple comparisons test was used to identify specific pairwise differences between the three groups after finding significant differences with ANOVA. The Chi-square test was also used to compare the distribution of categorical variables like sex between the groups.</p><p><strong>Results: </strong> All interventions significantly reduced PI, BOP, PPD, and CAL, but resveratrol and chlorhexidine had a higher significant effect than placebo except for CAL without a significant difference between them. All mouthwashes significantly reduced the salivary concentration of IL-6. However, resveratrol and chlorhexidine had a significantly higher effect than placebo, while the concentration of RANKL was decreased in all groups without a significant difference between them. The participants' responses to the mouthwash questionnaire showed that resveratrol and chlorhexidine had the same feedback without significant differences.</p><p><strong>Conclusion: </strong> Resveratrol-containing mouthwash could be used as an alternative to chlorhexidine as an adjunct to nonsurgical periodontal treatment of periodontitis.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142282543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Salivary Profile in Oral Submucous Fibrosis: A Scoping Review.","authors":"Fatma Yasmin Mahdani, Ajiravudh Subarnbhesaj, Nurina Febriyanti Ayuningtyas, Meircurius Dwi Condro Surboyo, Reiska Kumala Bakti, Desiana Radithia, Dimas Bayu Paramananda, Ina Indriyani, Fatimah Fauzi Basalamah","doi":"10.1055/s-0044-1788711","DOIUrl":"https://doi.org/10.1055/s-0044-1788711","url":null,"abstract":"<p><p>Diagnosing oral submucous fibrosis (OSMF) is invariably challenging. The disease can be detected after reaching its final stage and requires complex treatment. Changes in its salivary profile can be used as a reference to see this disorder and as a basis for diagnostic prediction. This study is aimed to analyze the salivary profile as a diagnosis marker in patients with OSMF. The study using Preferred Reporting Items for Systematic Reviews and Meta-analyses was conducted using PubMed, Science Direct, and Scopus databases. A thorough literature search between 1991 and 2023 was performed. Twenty-eight full-text articles were reviewed in detail. Twenty-eight articles were included; a total of 929 patients of OSMF and 826 controls were found. The scoping review showed that levels of salivary protein (including lactate hydrogenase, immunoglobulin G, immunoglobulin A, S1007A protein, 8-hydroxydeoxyguanosine, 8-isoprostane, malondialdehyde, matrix metalloproteinase-12, salivary C-reactive protein, fibrinogen producing factor, salivary miRNA-21, and salivary lipids [cholesterol, high-density lipoprotein, triglyceride) were higher in OSMF. Meanwhile, trace elements (vitamin C, vitamin E, iron, zinc, and magnesium) were lower; only copper was higher in OSMF patients. Alteration in salivary components such as protein, lipid, and trace elements detection can be a basis for providing a noninvasive supportive examination and thus be used as a diagnosis marker of OSMF.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141893213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A Comparison of Esthetic Preferences on Female Skeletal Class II Alterations among Laypeople of Different Facial Profiles.","authors":"Wiwan Tipyanggul, Chidsanu Changsiripun, Niramol Chamnannidiadha","doi":"10.1055/s-0044-1788654","DOIUrl":"https://doi.org/10.1055/s-0044-1788654","url":null,"abstract":"<p><strong>Objectives: </strong> This study aimed to investigate the influences of assessors' different personal profiles on the esthetic perception of Class II facial profile corrections and the agreement between profile and silhouette images.</p><p><strong>Materials and methods: </strong> A profile photo of a female with skeletal Class II was digitally altered into three profile and three silhouette images (most pronounced Class II division 1 characteristic, more retruded upper lip position, and more protruded mandibular position). Ninety-six laypeople from three facial profile groups (straight, convex, and concave profiles) chose these images for facial attractiveness. Data were analyzed using an SPSS program. Cohen's kappa coefficient and intraclass correlation coefficients were applied to determine intraparticipant and intra-examiner reliabilities. Chi-square tests were used to test between-group preferences and the relationship of profile preference with other factors. Cohen's kappa coefficient was used to test the agreement in selecting profile and silhouette images (<i>p</i> = 0.05).</p><p><strong>Results: </strong> All groups favored profiles with a protruded mandibular position (11-degree facial contour angle [FCA] and 91-degree nasolabial angle [NLA]). Despite facial profile differences, preference remained consistent (<i>p</i> = 0.649). The convex group showed a stronger inclination toward an untreated-simulating profile (17-degree FCA and 91-degree NLA). Preferences were consistent regardless of sex (<i>p</i> = 0.198) and education (<i>p</i> = 0.105). The percentage of agreement between profile and silhouette images in the total sample was 67.71% (kappa = 0.386). All groups of participants chose the more retruded upper lip position (17-degree FCA and 107-degree NLA) profile in silhouette more than in photograph.</p><p><strong>Conclusion: </strong> All groups preferred a mandibular advancement-simulating profile. Using the photographs or silhouettes to assess the esthetic preference resulted in a similar trend. However, the flatter profile was more preferred in silhouette than in photograph.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141792255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Precision of All-on-Four Implant Position Recorded from Three Different CBCT Machines.","authors":"Osamah Alsulimani, Abdulrahman Alhaddad, Mosa Altassan, Asmaa Bukhari, Lulu Munshi, Ghalia Sabir","doi":"10.1055/s-0044-1788613","DOIUrl":"https://doi.org/10.1055/s-0044-1788613","url":null,"abstract":"<p><strong>Objective: </strong> To investigate the dimensional discrepancy and degree of deviation of All-on-Four implant position between different cone-beam computed tomography (CBCT) machines.</p><p><strong>Materials and methods: </strong> Four implants (4.5 × 10 mm Superline II, Dentium, South Korea) were placed in an All-on-Four style in an artificial mandible. The jaw was radiated 30 times using three different CBCT machines (Rainbow CT, Dentium; Veraview X800, Morita, Japan; Planmeca Viso G3, Planmeca OY, Finland). A total of 30 Digital Imaging and Communications in Medicine (DICOM) files were exported, <i>n</i> = 10. All-on-Four implants from each DICOM file were segmented and exported as an STL file (three-dimensional image) using Blue Sky Plan software (version 4.12.13/Blue Sky Bio, United States). All-on-Four implant zone dimensions (<i>X</i>, <i>Y</i>, and <i>Z</i> axes) and the total degree of deviation between All-on-Four implants per CBCT machine were measured using Autodesk Meshmixer software (version 3.5.474/California, United States). The data distribution's normality and variances' equality were tested with Shapiro-Wilk's and Levene's tests, respectively (<i>p</i>-value < 0.05). Data were analyzed using Brown-Forsythe one-way analysis of variance and Tamhane's post hoc tests to compare the differences between the groups (<i>p</i>-value <0.05).</p><p><strong>Results: </strong> The respective <i>X</i>, <i>Y</i>, and <i>Z</i> mean dimensions of the All-on-Four implant zone were: Dentium (34.95, 14.71, and 9.97); Morita (34.88, 14.74, and 10.56); and Planmeca (34.73, 15.15, and 12.33). Significant differences between CBCT machines were found in all axes (<i>p</i>-value < 0.05); however, the <i>Z</i>-axis had the most differences. Notably, Planmeca exhibited the highest standard deviation (SD) in all axes (0.16-0.35), exhibiting the lowest consistency in the CBCT machines' readings. The Dentium exhibited the lowest deviation in the implant position, with the lowest SD (0.61). A significant difference in the total degree of deviation was spotted when only Morita was included in the comparison (<i>p</i>-value < 0.05).</p><p><strong>Conclusion: </strong> This study's findings are of significant importance as they reveal that the implant position recorded from the CBCT machines was most discrepant in the buccolingual dimension (<i>Z</i>-axis). Planmeca exhibited the least implant-dimensional accuracy of the CBCT machines, while Dentium exhibited the highest implant position accuracy. These results could significantly impact the choice of CBCT machine for implant placement, especially since an accurate CBCT image is crucial for digital implant planning.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141751429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Oussama Benyounes, Sana Bekri, Sameh Belgacem, Amel Labidi, Mehdi Khemis, Lamia Mansour
{"title":"Oral Colonization by Different Candida Species: First Comparative Study between Denture and Nondenture Wearers in Tunisia.","authors":"Oussama Benyounes, Sana Bekri, Sameh Belgacem, Amel Labidi, Mehdi Khemis, Lamia Mansour","doi":"10.1055/s-0044-1787819","DOIUrl":"https://doi.org/10.1055/s-0044-1787819","url":null,"abstract":"<p><strong>Objective: </strong> This study aimed to compare different <i>Candida</i> species present in patients with and without removable dentures to identify alterations in biofilm composition following denture wear within a Tunisian population.</p><p><strong>Materials and methods: </strong> A cross-sectional study was conducted, comprising a group of patients wearing removable dentures (test group) and a control group without dentures. In the test group, two mycological samples were obtained: one from the prosthetic intaglio and another from the osteomucosal area bearing the denture. For the control group, mycological samples were collected from the oral mucosa. The collected swabs were cultured on CHROMagar <i>Candida</i> medium, and yeast counts were quantified as colony forming units (CFUs). <i>Candida</i> species were identified through chromogenic analysis.</p><p><strong>Statistical analysis: </strong> The normality of quantitative variables was evaluated using the Kolmogorov-Smirnov's test. To compare means and ranks between the test and control groups, the independent samples <i>t</i>-test and the Mann-Whitney's <i>U</i> test were employed, respectively. Qualitative variables were compared using Fisher's exact test. Statistical significance was determined at a critical uncertainty value of <i>p</i> < 0.05.</p><p><strong>Results: </strong> A total of 150 participants were involved in this study, with 75 patients in each group. Wearing an acrylic removable denture was found to increase the number of detected <i>Candida</i> species (<i>p</i> < 0.001) and significantly increases the overall growth of <i>Candida</i> spp. (<i>p</i> = 0.001). Specifically, the numbers of CFUs of <i>Candida tropicalis</i> and <i>Candida glabrata</i> were elevated in denture wearers (<i>p</i> < 0.001).</p><p><strong>Conclusion: </strong> Findings stemming from this study indicate that removable dentures promote the growth of <i>Candida</i> species. This can be a predisposing factor for <i>Candida</i>-associated denture stomatitis in cases of poor oral hygiene or compromised immunity. Therefore, it is imperative to emphasize the fabrication of high-quality dentures and the implementation of rigorous postdenture maintenance protocols to prevent or limit <i>Candida</i> infection.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141751428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Valeria Widita Wairooy, Dini Asrianti Bagio, Anggraini Margono, Ingrid Amelia
{"title":"In vitro Analysis of DSPP and BSP Expression: Comparing the Odontogenic Influence of Bio-C Repair and Biodentine in hDPSCs.","authors":"Valeria Widita Wairooy, Dini Asrianti Bagio, Anggraini Margono, Ingrid Amelia","doi":"10.1055/s-0044-1786984","DOIUrl":"https://doi.org/10.1055/s-0044-1786984","url":null,"abstract":"<p><strong>Objectives: </strong> This study compared the ability of BIO-C Repair (BC) and Biodentine (BD) in relation to odontogenic differentiation by evaluating the dentin sialophosphoprotein (DSPP) and bone sialoprotein (BSP) expression and mineral deposition of human dental pulp stem cells (hDPSCs).</p><p><strong>Materials and methods: </strong> BC and BD were pulverized and sterilized (ISO 10993-5:2009). The hDPSCs were the result of primary cultures that were 80% confluent (having gone through the stem cell marker tests CD90 98%, CD105 99.7%, CD73 94%, and LinNeg 0.5%) and reached P2-3 by means of serum starvation for 24 hours. This study involved seven groups, in which the hDPSCs were cultured on osteogenic media with the addition of either BD (Septodont, United States) at concentrations of 1:1, 1:2, or 1:5; BC (Angelus, Brazil) at concentrations of 1:1, 1:2, or 1:5; or the negative control (Dulbecco's modified eagle medium + osteogenic media). The hDPSC differentiation was determined via enzyme-linked immunosorbent assays of DSPP and BSP expression performed on days 7 and 14 and alizarin red staining performed on day 21.</p><p><strong>Statistical analysis: </strong> The data were analyzed using a one-way analysis of variance, followed by Tamhane's post hoc test, to compare the differences between groups. The <i>t</i>-test dependent was also used to identify differences between groups.</p><p><strong>Results: </strong> BC and BD at 1:1 concentration, there was a statistically significant difference in DSPP and BSP expression. However, at concentrations of 1:2 and 1:5, there was no significant difference observed in either duration of observation (<i>p</i> > 0.05). The highest DSPP and BSP concentrations after 7 and 14 days of observation were observed with BD and BC at 1:5 concentration (6.6-6.71 and 13.20-13.47 ng/mL).</p><p><strong>Conclusion: </strong> The study shows that BC is as effective as BD in enhancing DSPP and BSP expression and mineral deposition in hDPSCs. The 1:5 concentration of BC showed the highest levels of DSPP and BSP expression and mineral deposition.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141727074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Arrad Ibrahim Rambey, Nina Ariani, Lindawati Soetanto Kusdhany
{"title":"Cross-Cultural Adaptation of The Indonesian Version Functional Assessment of Denture Instrument as a Measuring Tool for Complete Denture Functional Quality.","authors":"Arrad Ibrahim Rambey, Nina Ariani, Lindawati Soetanto Kusdhany","doi":"10.1055/s-0044-1786843","DOIUrl":"https://doi.org/10.1055/s-0044-1786843","url":null,"abstract":"<p><strong>Objective: </strong> Making good quality dentures is necessary to avoid patients' discomfort when adapting to new dentures. Several studies regarding the assessment of the quality of dentures have been carried out in many other countries, such as using the Functional Assessment of Dentures (FAD) by Anastassiadou. However, studies have yet to be conducted in Indonesia. This study aims to obtain a valid and reliable instrument to measure the quality of complete dentures (CDs). This study also aims to find out whether the functional quality of a CD measured with the Indonesian version of the FAD Instrument (PFGT) can affect patient satisfaction in receiving CD treatment (measured with Indonesian version of Patient's Denture Assessment [PDA-Id]) and to find contributing factors to the functional quality of a CD.</p><p><strong>Materials and methods: </strong> The study was conducted in two stages. The first stage was a qualitative study using cross-cultural adaptation methods and focus group discussions with experts. The second stage was a quantitative test with a total of 40 subjects for statistical analysis in the form of a kappa test, test-retest, Kuder-Richardson (KR) 20, and a correlation test between PDA-Id and PFG. Multivariate analysis was done to analyze contributing factors to the functional quality of CD.</p><p><strong>Results: </strong> The final instrument was obtained from the qualitative test, which was tested for content and face validation. The interrater kappa test result of 0.828 shows an almost perfect agreement. The results of the intrarater test-retest (0.564; <i>p</i> > 0.05; intraclass correlation coefficient 0.889) showed excellent instrument stability. The results of the internal consistency test with Kuder-Richardson 20 (1.08; KR > 1) showed good internal consistency. The correlation test results between PFGT and PDA-Id (0.044; <i>p</i> < 0.05) showed a positive correlation. Multivariate analysis showed a relationship between the quality of the CD, the length of time the CD was used, and the patient's satisfaction with CD treatment.</p><p><strong>Conclusion: </strong> The PFGT instrument is considered a valid and reliable tool to measure the functional quality of a denture that can distinguish between good and bad quality.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141727072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}