European Journal of Dentistry最新文献

{"title":"Analysis of Tissue Repair of a New Cement Based on Calcium and Strontium Aluminates: A Triple-Blinded, Randomized, Controlled Trial in an Animal Model.","authors":"Elizandra Silva da Penha, Nonato Amorim de Farias Filho, Luanna Abílio Diniz Melquíades de Medeiros, Rosana Araújo Rosendo, Marco Antônio Dias da Silva, Willams Teles Barbosa, Raúl García-Carrodeguas, Miguel Angel Rodríguez, Eliseu Aldrighi Münchow, Rogério Lacerda-Santos, Marcus Vinícius Lia Fook","doi":"10.1055/s-0044-1786875","DOIUrl":"10.1055/s-0044-1786875","url":null,"abstract":"<p><strong>Objective: </strong> The focus of this triple-blind randomized study was to evaluate the biocompatibility of a new root canal filling sealer (RCFS) based on tristrontium aluminate and dodecacalcium hepta-aluminate in living tissue.</p><p><strong>Material and methods: </strong> Forty-five Wistar rats (<i>Rattus norvegicus</i>) were divided into three groups: control (polyethylene), sealer (Bio-C Sealer, Londrina, PR, Brazil), and experimental (tristrontium aluminate and dodecacalcium hepta-aluminate). The tissues were analyzed under an optical microscope to assess different cellular events at different time intervals (7, 15, and 30 days).</p><p><strong>Statistical analysis: </strong> Data were analyzed using the Kruskal-Wallis and Dunn (<i>p</i> < 0.05) tests.</p><p><strong>Results: </strong> In the initial period, a moderate inflammatory infiltrate was observed, similar between the endodontic cements groups (<i>p</i> = 0.725). The intensity of the infiltrate decreased with time, with no significant difference among the groups (<i>p</i> > 0.05). The number of young fibroblasts was elevated in all groups evaluated at 7 days. The experimental group showed the highest number of cells at all time intervals, but the difference with the sealer group at 7 (<i>p</i> = 0.001) and 15 days (<i>p</i> = 0.002) and the control group at 30 days was not significant (<i>p</i> = 0.001). Regarding tissue repair events, the amount of collagen fibers increased over the experimental intervals, with no significant difference between the sealer and control groups (<i>p</i> > 0.05).</p><p><strong>Conclusion: </strong> The experimental RCFS based on calcium and strontium aluminates proved to be biocompatible for use in close contact with periapical tissue, inducing a low inflammatory reaction and favoring rapid tissue repair.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":"1076-1084"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11479734/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141081092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interprofessional Education in Saudi Arabia: A Call to Action.","authors":"Ahmed Yaseen Alqutaibi, Rawda Awad, Mohamed M Rahhal, Muhammad S Zafar","doi":"10.1055/s-0044-1788321","DOIUrl":"10.1055/s-0044-1788321","url":null,"abstract":"","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":"963-964"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11479745/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141626457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Periodontal Diseases on Oral Health-Related Quality of Life: A Study with a Condition-Specific Questionnaire in Croatian Population.","authors":"Aleksandar Pupovac, Davor Kuiš, Ivana Mišković, Jelena Prpić","doi":"10.1055/s-0044-1785534","DOIUrl":"10.1055/s-0044-1785534","url":null,"abstract":"<p><strong>Objectives: </strong> The aim of this study was to translate and validate the condition-specific Oral Health Impact Profile (OHIP) in the Croatian cultural context and assess the impact of periodontal diseases and nonsurgical periodontal therapy on quality of life (QoL).</p><p><strong>Materials and methods: </strong> A cross-sectional study was carried out on 150 individuals: 50 periodontally healthy, 50 with gingivitis, and 50 with periodontitis who self-administrated the OHIP. The participants' age ranged between 18 and 71 years, with the median age of 45 (34-57) years. Forty-seven percent of the participants were females. The validity and reliability of the Croatian OHIP version were tested. The impact of gingivitis and periodontitis on QoL was assessed. Changes in QoL induced by nonsurgical periodontal therapy in 20 patients with periodontitis were analyzed.</p><p><strong>Statistical analysis: </strong> Categorical data were presented by absolute and relative frequencies. The normality of the distribution of continuous variables was tested by the Shapiro-Wilk test. Continuous data were described by the median and the limits of the interquartile range (IQR). Differences in continuous variables between two independent groups were tested with the Mann-Whitney <i>U</i> test, and between three groups with the Kruskal-Wallis test (post hoc Conover). The Wilcoxon signed-rank test was used to examine the differences in the total score before and after therapy. All <i>p</i> values were two-sided. The level of significance was set at alpha of 0.05.</p><p><strong>Results: </strong> The analysis detected a single-factor structure that explained for the 56.9% of the variance. Cronbach's alpha value was 0.937, which indicated an excellent internal consistency. Overall OHIP score reported a strong correlation with the subjective estimate of periodontal problems (Rho = 0.92; <i>p</i> < 0.001). Test-retest reliability was high (<i>r</i> = 0.984; <i>p</i> < 0.001). The periodontitis group had the highest OHIP score (28 [23-34]), followed by the gingivitis group (14 [12-20]) and the periodontally healthy group (9 [5-11]; <i>p</i> < 0.001). Nonsurgical periodontal therapy significantly improved the QoL in those with periodontitis (<i>p</i> < 0.001).</p><p><strong>Conclusion: </strong> The condition-specific Croatian version of the OHIP instrument can be considered adequate to measure the impact of periodontal diseases on oral health-related QoL. Periodontal diseases, especially periodontitis, have a negative effect on the patient's QoL. Nonsurgical periodontal treatment can improve patients' QoL.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":"1172-1178"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11479721/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141893212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of the Shear Bond Strength Using Primers with Different Application Numbers on Dental Zirconia.","authors":"Suphakit Opatragoon, Awiruth Klaisiri, Tool Sriamporn, Niyom Thamrongananskul","doi":"10.1055/s-0043-1777821","DOIUrl":"10.1055/s-0043-1777821","url":null,"abstract":"<p><strong>Objective: </strong> This study examined the effect of the number of phosphate-containing primer applications on the shear bond strength (SBS) of zirconia to resin cement.</p><p><strong>Materials and methods: </strong> 315 square specimens (10 × 10 × 4 mm³) were manufactured from Cercon ht presintered zirconia blocks. Alumina particles were used to sandblast zirconia specimens. These specimens were randomly divided into six primer-based groups: No primer application (NP), CLEARFIL CERAMIC PRIMER (C), PANAVIA V5 Tooth Primer (T), M&C PRIMER (MC), Monobond N (MN), and Z-PRIME plus (Z), and then separated into application number (1-4) groups (excluding NP). Each specimen was bonded with resin cement. The SBS was measured using a universal testing machine. The debonded surface was examined with a stereomicroscope.</p><p><strong>Statistical analysis: </strong> The SBSs were analyzed using two-way analysis of variance.</p><p><strong>Results: </strong> Applying the primer twice exhibited the highest SBSs in each group, with significant differences in the T, MN, and Z groups. However, the SBS in the MC group was significantly lower on the second application. One-hundred percent adhesive failure was observed in all groups.</p><p><strong>Conclusion: </strong> Within the limitations of this study, prior to cementation, the sandblasted zirconia surface should be applied twice with a phosphate-containing primer other than MC to maximize the SBS at the zirconia-resin cement interface.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":"1004-1011"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11479738/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141626426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Potential of QP3VH-Chitosan Peptide as Biomimetic Remineralization in Early Dental Caries Treatment: An In Vitro Study.","authors":"Prima Agusmawanti, Diatri Nari Ratih, Nunuk Purwanti, Tri Joko Raharjo","doi":"10.1055/s-0044-1782189","DOIUrl":"10.1055/s-0044-1782189","url":null,"abstract":"<p><strong>Objectives: </strong> The development of remineralization biomimetics using organic peptide molecules is expected to resemble the hydroxyapatite (HA) mineralization process in tooth enamel. The development of an amelogenin derivative peptide combined with antimicrobial peptide was designed, resulting in QP3VH. This combination then was mixed with chitosan as a carrier. This study aimed to evaluate the biomimetic efficacy of QP3VH as a remineralizing agent combined with chitosan.</p><p><strong>Materials and methods: </strong> Fifty deciduous mandibular incisor enamel samples were used in this study. The artificial enamel lesions were created on a buccal surface and were randomly assigned to five groups of 10 each according to the remineralizing agent used: QP3VH, NaF, QP3VH + NaF, QP3VH + CS (QP3VH + chitosan), and saline distilled water (SDW). Each group was performed pH cycling for seven days. Enamel surface morphology and evaluation of mineral content Ca/P (calcium and phosphate) using scanning electron microscopy and energy dispersive X-ray analysis. The assessment was carried out, after demineralization, and after application with remineralization agents.</p><p><strong>Statistical analysis: </strong> Data were analyzed using a one-way analysis of variance followed by least significance difference post-hoc test. The paired <i>t</i>-test was utilized to compare the demineralization and remineralization results. The significance level used was 95%.</p><p><strong>Results: </strong> The remineralized group exhibited a significant increase in calcium and phosphate content on the enamel surface (<i>p</i> <0.05), and QP3VH + CS produced the maximum Ca/P mass percent after remineralization.</p><p><strong>Conclusion: </strong> Combining QP3VH with chitosan produces greatest remineralization than QP3VH, QP3VH + NaF, Naf, and SDW; therefore, QP3VH peptide has potential as a remineralizing agent, in the future.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":"1149-1156"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11479723/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140956944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Capsaicin on Migration and Alkaline Phosphatase Activity of Dental Pulp Cells.","authors":"Kittipot Khonglim, Boontharika Chuenjitkuntaworn, Yukihiko Tamura, Pornpoj Fuangtharnthip","doi":"10.1055/s-0044-1782191","DOIUrl":"10.1055/s-0044-1782191","url":null,"abstract":"<p><strong>Objectives: </strong> Dental pulp, a specialized mesenchymal tissue within teeth, is pivotal in dental health and tissue repair. Capsaicin, the primary pungent component of chili peppers, is known for its diverse pharmacological properties. While capsaicin's effects on various cell types have been studied, its impact on dental pulp cells remains relatively unexplored. This study investigated the influence of pure capsaicin extract on dental pulp cell behavior, focusing on cell viability, proliferation, migration, and alkaline phosphatase (ALP) activity.</p><p><strong>Materials and methods: </strong> Capsaicin solution was prepared and diluted to various concentrations (1 nM, 0.01 µM, 0.1 µM, 1 µM, 10 µM, and 100 µM), then was tested on rat dental pulp cells (RPC-C2A). Cell viability and proliferation were assessed using the MTT assay. Boyden chamber tests and wound healing were used for evaluating cell migration. The activity of ALP was determined to show cell function during dental pulp repair.</p><p><strong>Statistical analysis: </strong> The data were analyzed using a one-way analysis of variance or an independent-sample Kruskal-Wallis, followed by multiple comparison tests.</p><p><strong>Results: </strong> Capsaicin of 100 µM exhibited cytotoxicity, whereas those with lower concentrations stimulated cell proliferation. Wound healing assays revealed increased cell migration, particularly when cultured with 1 nM capsaicin (<i>p</i> = 0.002). Boyden chamber assays demonstrated enhanced cell invasion without statistical significance. ALP activity of dental pulp cells increased significantly at 1 nM (<i>p</i> < 0.001) and 1 µM (<i>p</i> = 0.021) capsaicin concentrations, indicating potential dentinogenesis and pulp repair.</p><p><strong>Conclusion: </strong> Capsaicin of lower concentrations, less than 10 µM, is likely to promote proliferation, migration, and ALP activity of dental pulp cells. Our findings offer potential applications for capsaicin as a medication for dental pulp repair.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":"1157-1163"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11479730/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140852431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of Stress Distribution and Displacement Based on the Miniscrew Positions of the Palatal Slope Bone-borne Expander: A Finite Element Study.","authors":"Chakree Leeisaramas, Nattapon Chantarapanich, Samroeng Inglam, Kanlaya Insee","doi":"10.1055/s-0043-1777823","DOIUrl":"10.1055/s-0043-1777823","url":null,"abstract":"<p><strong>Objectives: </strong> This study aimed to investigate the stress distribution pattern of the palatal slope bone-borne expander on the maxillary area according to a different anteroposterior position of anchored miniscrews using finite element analysis.</p><p><strong>Materials and methods: </strong> Nasomaxillary stereolithography files with three different anteroposterior anchored miniscrew positions of the palatal slope bone-borne expander were determined as model A, B, and C. Each model consists of four supported miniscrews. Model A: two anterior miniscrews were located between the maxillary canine and the first premolar, and two posteriors between the second premolar and the first molar. Model B: two anteriors were between the lateral incisor and the canine, and two posteriors were the same as in model A. Model C: two anteriors were the same as in model A, and two posteriors were distal to the first molar. One turn of expander screws was applied. Maximum principal stress, equivalent elastic strain, equivalent von Mises stress, and transverse displacement were evaluated.</p><p><strong>Results: </strong> The maximum principal stress was mostly found at the bone-miniscrew interface. Model A exhibited an intersecting area of stress between the supported miniscrews. The highest value of principal stress was in model B, while model C showed a uniform distribution pattern. The elastic strain pattern was similar to the principal stress in all models. The highest value of equivalent von Mises stress was located on the expander screw. The largest amount of transverse displacement of teeth was in model A, while model C exhibited a more consistent transverse displacement than other models. Vertical displacement of posterior teeth was also noticed.</p><p><strong>Conclusion: </strong> Based on the result, it revealed that the various anteroposterior miniscrew placements of the palatal slope bone-borne expander had various patterns of stress distribution and resulted in various outcomes. It may be inferred that model A's miniscrew location was advantageous for obtaining expansion quantities, but model C's miniscrew position was advantageous for maintaining consistent biomechanics.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":"1012-1021"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11479739/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140331663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anticancer Potential of Quercetin on Oral Squamous Cell Carcinoma: A Scoping Review and Molecular Docking.","authors":"Dhona Afriza, Utmi Arma, Raefany Faslah, Wastuti Hidayati Suriyah","doi":"10.1055/s-0044-1789016","DOIUrl":"https://doi.org/10.1055/s-0044-1789016","url":null,"abstract":"<p><p>Oral squamous cell carcinoma (OSCC) is a type of cancer that has a low survival rate and high recurrence and metastasis rates. To date, there is still no effective treatment for OSCC. Various types of cancer, including OSCC, have reported quercetin to act as an anticancer agent, but there is no clear research data on how it may affect OSCC. To determine the anticancer potential of quercetin in OSCC, we conducted a scoping review, and to determine the interaction of quercetin with one of the proteins that plays a role in carcinogenesis, namely, BCL-2, we conducted molecular docking. The scoping review process was conducted based on the Preferred Reporting Items for Systematic Reviews and Meta-Analyses Extension for Scoping Reviews. The scoping review was searched by collecting articles related to the research topic in Google Scholar, PubMed, ScienceDirect, Cochrane, and EBSCOhost databases. All of the literature records found during the search were imported into the Mendeley software to remove duplication. Nine studies were generated after the titles and abstracts were reviewed according to the inclusion and exclusion criteria. After the full-text screening, no studies were excluded, leaving nine publications determined to be eligible for inclusion in the scoping review. Quercetin showed effects on inhibiting cancer invasion, migration, proliferation, and many protein expressions, as well as increasing cell apoptosis. Molecular docking was done for quercetin and BCl-2 protein. Doxorubicin was utilized as a comparison ligand. The <i>in silico</i> study was utilized using AutoDock Vina, AutoDock Tools 1.5.6, Biovia Discovery Studio 2021, and PyMol. Molecular docking indicated quercetin has a strong binding affinity with BCl-2 protein (ΔG -7.2 kcal/mol). Both scoping review and molecular docking revealed that quercetin is a promising candidate for anticancer agent.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142344213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Variability in Positions and Factors Contributing to Surgical Difficulty of Impacted Third Molars.","authors":"Endang Sjamsudin, Anggun Rafisa, Nuroh Najmi","doi":"10.1055/s-0044-1788796","DOIUrl":"https://doi.org/10.1055/s-0044-1788796","url":null,"abstract":"<p><strong>Objectives: </strong> This study aimed to provide valuable insights into the variability of third molar positions and factors influencing their surgical time and technique.</p><p><strong>Materials and methods: </strong> This cross-sectional study included a total of 48 eligible participants, aged 18 to 45 years, diagnosed with impacted teeth, and who had undergone surgery. Exclusion criteria comprised the absence of the second molar, the presence of systemic diseases, a history of radiation therapy, and pregnancy or lactation. Participants completed a questionnaire covering demographic data, physical metrics, and information on systemic conditions and disorders. Preoperative assessments included vital sign measurements. Panoramic imaging was employed to evaluate the third molar distance to the occlusal plane, degrees of angulation, eruption space, and distance to the alveolar inferior canal. Surgical difficulty in this study was measured by two outcomes: surgical time and technique.</p><p><strong>Statistical analysis: </strong> To assess differences in impacted third molar positions among groups, Independent samples <i>t</i>-test and One-way analysis of variance were used for normally distributed data without outliers; otherwise, the Mann-Whitney U test and Kruskal-Wallis H test were utilized. The Spearman's rank correlation was utilized to explore relationships between vital signs, impacted third molar positions, surgical time, and surgical technique.</p><p><strong>Results: </strong> There were no significant differences in third molar positions between age and body mass index groups. Significant differences in distance to the occlusal plane were observed between third molars in quadrants 2 and 3 (<i>p</i> = 0.002) and quadrants 2 and 4 (<i>p</i> = 0.005). A significant difference in eruption space was found between sexes (<i>p</i> = 0.016). A significant negative correlation was discovered between surgical time and respiration rate per minute (<i>p</i> = 0.028).</p><p><strong>Conclusion: </strong> This study found that males have greater third molar eruption space than females, and maxillary third molars have a greater distance to the occlusal plane compared with mandibular third molars. The importance of vital signs as contributing factors to surgical difficulty is highlighted, emphasizing their relevance in clinical practice.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142344214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and Characterization of Key Genes Associated with Amelogenesis.","authors":"Tahsinul Haque, Fatema Akhter, Nourelhoda Alim, Abdullah Nabhan, Fawzia Al Kahtani, Abdullah Mohammed Sambawa","doi":"10.1055/s-0044-1787958","DOIUrl":"https://doi.org/10.1055/s-0044-1787958","url":null,"abstract":"<p><strong>Objectives: </strong> The identification of key genes associated with amelogenesis would be helpful in finding solutions to genetic disorders in oral biology. The study aimed to use <i>in silico</i> analysis to identify the key genes involved in tooth development associated with preameloblasts (PABs) and secretory ameloblasts (SABs).</p><p><strong>Material and methods: </strong> The data was subjected to quality analysis and uniform manifold approximation and projection analysis. To examine the distribution of the genes and identify important upregulated loci, a <i>p</i>-value histogram, a quantile plot, a mean difference and mean-variance plot, and a volcano plot were generated. Finally, protein-protein interaction and gene enrichment analyses were performed to determine the ontology, relevant biological processes, and molecular functions of selected genes.</p><p><strong>Results: </strong> A total of 157 genes were found to be significant in the PAB versus SAB comparison. HIST1H31 revealed strong interaction with HIST1H2BM, and EXO1, ASPM, SPC25, and TTK showed strong interactions with one other. The STRING database revealed that NCAPG, CENPU, NUSAP1, HIST1H2BM, and HIST1H31 are involved in biological processes. NCAPG, CENPU, SPC25, ETV5, TTK, ETV1, FAM9A, NUSAP1, HIST1H2BM, and HIST1H31 are involved in cellular components.</p><p><strong>Conclusion: </strong> The TTK, NUSAP1, CENPU, NCAPG, FAM9A, ASPM, SPC25, and HIST1H31 genes demonstrate functions in cell division. These genes might play a role in ameloblast development. These results will be useful in developing new methods to stimulate ameloblast development, which is essential for tooth regeneration and tissue engineering. However, more research is required to validate the functions of these genes and the genes with which they interact. A wide variety of genetic, epigenetic, and exogenous signaling factors regulate these genes and pathways throughout development and differentiation, cell fate, and behavior.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142282559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}