Valeria Widita Wairooy, Dini Asrianti Bagio, Anggraini Margono, Ingrid Amelia
{"title":"In vitro Analysis of DSPP and BSP Expression: Comparing the Odontogenic Influence of Bio-C Repair and Biodentine in hDPSCs.","authors":"Valeria Widita Wairooy, Dini Asrianti Bagio, Anggraini Margono, Ingrid Amelia","doi":"10.1055/s-0044-1786984","DOIUrl":"10.1055/s-0044-1786984","url":null,"abstract":"<p><strong>Objectives: </strong> This study compared the ability of BIO-C Repair (BC) and Biodentine (BD) in relation to odontogenic differentiation by evaluating the dentin sialophosphoprotein (DSPP) and bone sialoprotein (BSP) expression and mineral deposition of human dental pulp stem cells (hDPSCs).</p><p><strong>Materials and methods: </strong> BC and BD were pulverized and sterilized (ISO 10993-5:2009). The hDPSCs were the result of primary cultures that were 80% confluent (having gone through the stem cell marker tests CD90 98%, CD105 99.7%, CD73 94%, and LinNeg 0.5%) and reached P2-3 by means of serum starvation for 24 hours. This study involved seven groups, in which the hDPSCs were cultured on osteogenic media with the addition of either BD (Septodont, United States) at concentrations of 1:1, 1:2, or 1:5; BC (Angelus, Brazil) at concentrations of 1:1, 1:2, or 1:5; or the negative control (Dulbecco's modified eagle medium + osteogenic media). The hDPSC differentiation was determined via enzyme-linked immunosorbent assays of DSPP and BSP expression performed on days 7 and 14 and alizarin red staining performed on day 21.</p><p><strong>Statistical analysis: </strong> The data were analyzed using a one-way analysis of variance, followed by Tamhane's post hoc test, to compare the differences between groups. The <i>t</i>-test dependent was also used to identify differences between groups.</p><p><strong>Results: </strong> BC and BD at 1:1 concentration, there was a statistically significant difference in DSPP and BSP expression. However, at concentrations of 1:2 and 1:5, there was no significant difference observed in either duration of observation (<i>p</i> > 0.05). The highest DSPP and BSP concentrations after 7 and 14 days of observation were observed with BD and BC at 1:5 concentration (6.6-6.71 and 13.20-13.47 ng/mL).</p><p><strong>Conclusion: </strong> The study shows that BC is as effective as BD in enhancing DSPP and BSP expression and mineral deposition in hDPSCs. The 1:5 concentration of BC showed the highest levels of DSPP and BSP expression and mineral deposition.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":"220-226"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11750311/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141727074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Oussama Benyounes, Sana Bekri, Sameh Belgacem, Amel Labidi, Mehdi Khemis, Lamia Mansour
{"title":"Oral Colonization by Different Candida Species: First Comparative Study between Denture and Nondenture Wearers in Tunisia.","authors":"Oussama Benyounes, Sana Bekri, Sameh Belgacem, Amel Labidi, Mehdi Khemis, Lamia Mansour","doi":"10.1055/s-0044-1787819","DOIUrl":"10.1055/s-0044-1787819","url":null,"abstract":"<p><strong>Objective: </strong> This study aimed to compare different <i>Candida</i> species present in patients with and without removable dentures to identify alterations in biofilm composition following denture wear within a Tunisian population.</p><p><strong>Materials and methods: </strong> A cross-sectional study was conducted, comprising a group of patients wearing removable dentures (test group) and a control group without dentures. In the test group, two mycological samples were obtained: one from the prosthetic intaglio and another from the osteomucosal area bearing the denture. For the control group, mycological samples were collected from the oral mucosa. The collected swabs were cultured on CHROMagar <i>Candida</i> medium, and yeast counts were quantified as colony forming units (CFUs). <i>Candida</i> species were identified through chromogenic analysis.</p><p><strong>Statistical analysis: </strong> The normality of quantitative variables was evaluated using the Kolmogorov-Smirnov's test. To compare means and ranks between the test and control groups, the independent samples <i>t</i>-test and the Mann-Whitney's <i>U</i> test were employed, respectively. Qualitative variables were compared using Fisher's exact test. Statistical significance was determined at a critical uncertainty value of <i>p</i> < 0.05.</p><p><strong>Results: </strong> A total of 150 participants were involved in this study, with 75 patients in each group. Wearing an acrylic removable denture was found to increase the number of detected <i>Candida</i> species (<i>p</i> < 0.001) and significantly increases the overall growth of <i>Candida</i> spp. (<i>p</i> = 0.001). Specifically, the numbers of CFUs of <i>Candida tropicalis</i> and <i>Candida glabrata</i> were elevated in denture wearers (<i>p</i> < 0.001).</p><p><strong>Conclusion: </strong> Findings stemming from this study indicate that removable dentures promote the growth of <i>Candida</i> species. This can be a predisposing factor for <i>Candida</i>-associated denture stomatitis in cases of poor oral hygiene or compromised immunity. Therefore, it is imperative to emphasize the fabrication of high-quality dentures and the implementation of rigorous postdenture maintenance protocols to prevent or limit <i>Candida</i> infection.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":"206-213"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11750328/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141751428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Joo Ming Cheong, Nur Adlina A Halim, Norsyamimi Mohammad, Mangaiyarkarasi Sivagnanam, Mohamad Shafiq Mohd Ibrahim
{"title":"Establishing H Angle Hard Tissue and H Angle Soft Tissue Norms in Skeletal Class I Malay Adult Females and the Correlation between the H Angles and Visual Perception of Laypersons.","authors":"Joo Ming Cheong, Nur Adlina A Halim, Norsyamimi Mohammad, Mangaiyarkarasi Sivagnanam, Mohamad Shafiq Mohd Ibrahim","doi":"10.1055/s-0044-1787005","DOIUrl":"10.1055/s-0044-1787005","url":null,"abstract":"<p><strong>Objectives: </strong> This study aimed to establish the cephalometric norms of H angle soft tissue and H angle hard tissue of Malaysian Malay adult females, and to evaluate the correlation between H angles and visual perception in skeletal Class I Malay adult females.</p><p><strong>Materials and methods: </strong> Eighty-five lateral cephalograms of skeletal Class I (mean ANB value = 3.15 ± 0.77) Malaysian Malay female patients aged 20 to 40 years (mean age = 28.6 ± 5.86 degrees) taken from October 2017 to December 2021 were measured for H angle soft tissue and H angle hard tissue. Twenty silhouettes were then converted from cephalometric films and were rated according to facial convexity/concavity by 20 laypersons, with re-evaluation after 2 weeks for intra- and interexaminer reliabilities.</p><p><strong>Results: </strong> The means of the H angle soft tissue and H angle hard tissue were 15.75 ± 4.16 degrees and 11.64 ± 4.71 degrees, respectively. The intraexaminer reliability test for visual perception ranged from -0.89 to 0.99 indicating poor to excellent reliability, whereas the interexaminer reliability test was 0.82 indicating good reliability. A highly statistically significant association between the H angle soft tissue and H angle hard tissue (<i>r</i> = 0.938; <i>p</i> < 0.01) was found. There was no correlation between H angles and visual perception.</p><p><strong>Conclusion: </strong> The cephalometric norms of H angle hard tissue and H angle soft tissue in the Malaysian Malay female population were established: 11.64 degrees (±4.71 degrees) and 15.75 degrees (±4.16 degrees), respectively. There was a strong correlation between H angle soft tissue and H angle hard tissue among skeletal Class I Malay adult females. There was no correlation between H angles and the visual perception of laypersons.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":"214-219"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11750322/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141626454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Dealing with Evidence in Dental Professional Liability Lawsuits-General Recommendations for Dental Expert Witness Work Using the Example of Germany: A Narrative Review.","authors":"Hans Ulrich Brauer, Andreas Bartols","doi":"10.1055/s-0044-1788320","DOIUrl":"10.1055/s-0044-1788320","url":null,"abstract":"<p><p>Dental treatment can lead to disputes between patients and dentists, which are then brought before the civil courts as medical malpractice cases. The court regularly commissions a dental expert to answer questions of evidence in court. In the majority of cases, the expert is able to answer these questions based on his professional expertise and practical experience. In order to make the assessment comprehensible and credible for the judge and the parties, it can be helpful and reasonable to cite relevant literature references from dental textbooks and specialist journals. In individual cases, it may be necessary to carry out a systematic literature search on specific topics. Based on the situation in Germany, this narrative review makes recommendations of how to perform a literature search and make citations specifically for expert opinions that are generally applicable independent of national jurisdiction.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11750329/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141626453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fatma Makkeyah, Nesrine A Elsahn, Mahmoud M Bakr, Mahmoud Al Ankily
{"title":"Effect of Acidic Media on Surface Topography and Color Stability of Two Different Glass Ceramics.","authors":"Fatma Makkeyah, Nesrine A Elsahn, Mahmoud M Bakr, Mahmoud Al Ankily","doi":"10.1055/s-0044-1786842","DOIUrl":"10.1055/s-0044-1786842","url":null,"abstract":"<p><strong>Objective: </strong> The aim of this study was to determine the effect of two acidic beverages (orange juice and H<sub>3</sub>PO<sub>4</sub>-containing fizzy drink) on the surface topography and color stability of two commonly used computer-aided design/computer-aided manufacturing (CAD/CAM) ceramic materials.</p><p><strong>Materials and methods: </strong> Sixty samples of two CAD/CAM ceramic materials, lithium disilicate (IPS e-max CAD) and zirconia reinforced lithium silicate (Vita Suprinity), were prepared according to the manufacturer's instructions. The samples were immersed in one of three media (artificial saliva, orange juice and H<sub>3</sub>PO<sub>4</sub>-containing fizzy drink) and then stored in an incubator at 37 °C for 24 hours). Before and after immersion in different media, the surface roughness (Ra) of the samples was assessed using profilometer (JITAI8101 Surface Roughness Tester-Beijing Jitai Tech Detection Device Co. Ltd, China) and the color parameters were measured using VITA Easyshade Advance 4.01 (VITA shade, VITA made, VITA). Surface topography was observed using scanning electron microscope (SEM) and surface mineral content was compared before and after immersion. Paired sample <i>t</i>-test was used to determine the change in Ra before and after immersion. Two-way analysis of variance was used to determine the effect of different CAD/CAM materials and immersion media on the mean ∆Ra and mean ∆E of the studied groups. Tukey's honest significant difference posthoc test was used for multiple comparisons at a level of significance (α = 0.05).</p><p><strong>Results: </strong> A significant increase in Ra and a decrease in the color stability of the two investigated ceramic materials were detected after immersion in the acidic media than in artificial saliva. SEM and energy-dispersive X-ray results revealed the dissolution of the glassy matrix and the exposure of silicate crystals.</p><p><strong>Conclusion: </strong> The surface topography and color stability of glass ceramics are affected by the pH of different acidic media.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":"173-179"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11750313/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141081168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Evaluation of Physical Properties in Carboxymethyl Chitosan Modified Glass Ionomer Cements and the Effect for Dentin Remineralization: SEM/EDX, Compressive Strength, and Ca/P Ratio.","authors":"Aditya Wisnu Putranto, Ratna Meidyawati, Senyan Dwiseptyoga, Dicky Yudha Andhika Zikrullah","doi":"10.1055/s-0044-1786864","DOIUrl":"10.1055/s-0044-1786864","url":null,"abstract":"<p><strong>Objective: </strong> The aim of this article was to evaluate the effects of modifying glass ionomer cement (GIC) with carboxymethyl chitosan (CMC) on surface morphology and remineralization outcomes by examining dentin morphology and calcium ion composition changes.</p><p><strong>Materials and methods: </strong> Thirty holes in a cylindrical acrylic mold were filled with three groups of restorative materials: GIC, GIC modified with CMC (GIC-CMC) 5%, and GIC-CMC10%. The surface morphology of each group's materials was observed using scanning electron microscopy (SEM). The compressive strength measurement was performed using a universal testing machine. The dentin remineralization process was performed by applying GIC, GIC-CMC5%, and GIC-CMC10% materials for 14 days on demineralized dentin cavities treated with 17% ethylenediamine tetraacetic acid (EDTA) for 7 days. A morphological evaluation was conducted using SEM. The calcium ion composition and calcium-to-phosphorous (Ca/P) ratio were examined using an energy-dispersive X-ray (EDX).</p><p><strong>Statistical analysis: </strong> The one-way ANOVA and post-hoc Bonferroni test were used to evaluate the compressive strength within the three groups (<i>p</i> < 0.05). The Kruskal-Wallis and subsequent Mann-Whitney U tests were conducted to compare the four groups of calcium ions (<i>p</i> < 0.05).</p><p><strong>Results: </strong> The modification of GIC with CMC affected the morphological changes in the materials in the form of reduced porosity and increased fractures. A significant difference was found in compressive strength between the GIC-CMC modification materials of GIC-CMC5% and GIC-CMC10% and the GIC control group. The dentin tubule morphology and surface changes were observed after applying GIC, GIC-CMC5%, and GIC-CMC10% materials for 14 days, as evaluated by SEM. The EDX examination showed an increase in calcium ion content and hydroxyapatite formation (Ca/P ratio) after applying the GIC-CMC10% material.</p><p><strong>Conclusion: </strong> The surface porosity of the GIC modification material with the addition of CMC tended to decrease. However, an increase in cracked surfaces that widened, along with the rise in CMC percentage, was found. This modification also reduced the compressive strength of the materials, with the lowest average yield at 10% CMC addition. Therefore, the modification of GIC with CMC affects changes in morphology, calcium ion composition, and Ca/P ratio in demineralized dentin.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":"187-196"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11750317/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141626456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Arrad Ibrahim Rambey, Nina Ariani, Lindawati Soetanto Kusdhany
{"title":"Cross-Cultural Adaptation of The Indonesian Version Functional Assessment of Denture Instrument as a Measuring Tool for Complete Denture Functional Quality.","authors":"Arrad Ibrahim Rambey, Nina Ariani, Lindawati Soetanto Kusdhany","doi":"10.1055/s-0044-1786843","DOIUrl":"10.1055/s-0044-1786843","url":null,"abstract":"<p><strong>Objective: </strong> Making good quality dentures is necessary to avoid patients' discomfort when adapting to new dentures. Several studies regarding the assessment of the quality of dentures have been carried out in many other countries, such as using the Functional Assessment of Dentures (FAD) by Anastassiadou. However, studies have yet to be conducted in Indonesia. This study aims to obtain a valid and reliable instrument to measure the quality of complete dentures (CDs). This study also aims to find out whether the functional quality of a CD measured with the Indonesian version of the FAD Instrument (PFGT) can affect patient satisfaction in receiving CD treatment (measured with Indonesian version of Patient's Denture Assessment [PDA-Id]) and to find contributing factors to the functional quality of a CD.</p><p><strong>Materials and methods: </strong> The study was conducted in two stages. The first stage was a qualitative study using cross-cultural adaptation methods and focus group discussions with experts. The second stage was a quantitative test with a total of 40 subjects for statistical analysis in the form of a kappa test, test-retest, Kuder-Richardson (KR) 20, and a correlation test between PDA-Id and PFG. Multivariate analysis was done to analyze contributing factors to the functional quality of CD.</p><p><strong>Results: </strong> The final instrument was obtained from the qualitative test, which was tested for content and face validation. The interrater kappa test result of 0.828 shows an almost perfect agreement. The results of the intrarater test-retest (0.564; <i>p</i> > 0.05; intraclass correlation coefficient 0.889) showed excellent instrument stability. The results of the internal consistency test with Kuder-Richardson 20 (1.08; KR > 1) showed good internal consistency. The correlation test results between PFGT and PDA-Id (0.044; <i>p</i> < 0.05) showed a positive correlation. Multivariate analysis showed a relationship between the quality of the CD, the length of time the CD was used, and the patient's satisfaction with CD treatment.</p><p><strong>Conclusion: </strong> The PFGT instrument is considered a valid and reliable tool to measure the functional quality of a denture that can distinguish between good and bad quality.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":"48-54"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11750306/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141727072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Josuel Siqueira Azarias, Victor Augusto Alves Bento, Clóvis Lamartine de Moraes Melo Neto, Manuel Martin Adriazola Ique, Daniela Micheline do Santos, Marcelo Coelho Goiato
{"title":"What Is the Survival Rate of Implants Loaded Immediately with a Branemark Protocol Prosthesis? A Review.","authors":"Josuel Siqueira Azarias, Victor Augusto Alves Bento, Clóvis Lamartine de Moraes Melo Neto, Manuel Martin Adriazola Ique, Daniela Micheline do Santos, Marcelo Coelho Goiato","doi":"10.1055/s-0044-1787818","DOIUrl":"10.1055/s-0044-1787818","url":null,"abstract":"<p><p>The aim of this review was to determine the survival rate of implants loaded immediately with a Branemark protocol prosthesis. An electronic search was performed in the PubMed/MEDLINE database from 2006 to February 2024, using a combination of Medical Subject Headings descriptors: \"completely edentulous\" and \"immediate loading.\" Human clinical articles in English that evaluated the survival rate of implants loaded immediately with a Branemark protocol prosthesis after placement in the bone were included. A total of 546 articles were identified, of which 15 were included in this study. The 15 articles included in the literature showed a high implant survival rate (more than 93%) after immediate loading.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":"7-14"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11750347/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141626461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Antibacterial Efficacy of Graphene Nanoparticles against Enterococcus faecalis: In Vitro Study.","authors":"Omer Sheriff Sultan, Preena Sidhu, Kiran Rehman, Thiagrajan Madheswaran, Amalraj Fabian Davamani","doi":"10.1055/s-0044-1786863","DOIUrl":"10.1055/s-0044-1786863","url":null,"abstract":"<p><strong>Objective(s): </strong> This study compared the antimicrobial efficacy of nanographene (NG) particles with chlorhexidine (CHX) and calcium hydroxide (Ca(OH)<sub>2</sub>) against <i>Enterococcus faecalis</i>.</p><p><strong>Materials and methods: </strong> Forty extracted human mandibular premolar teeth were cleaned using a scaler, and the middle-third of the root (6 mm) was decoronated using a rotary diamond disk. The inner diameter of the teeth was made consistent using Gates Glidden Drills #3, treated with ethylene diamine tetra-acetic acid and sodium hypochlorite before sterilization. The samples were then contaminated with <i>E. faecalis</i> grown in Tryptic soy broth for 21 days. Tooth samples were then randomly divided into four groups: Group I (Control), untreated saline; Group II, Ca(OH)<sub>2</sub>; Group III, CHX; and Group IV, NG. The assessment of bacterial growth was carried out by harvesting dentin chips at the end of 1, 3, and 7 days. The colonies were physically counted and tabulated after 24 hours from seeding. Statistical analysis of the collected data was performed with analysis of variance and Tukey's post hoc test using SPSS Version 20.0.</p><p><strong>Results: </strong> The contaminated dentine blocks irrigated with NG (0.5 µg) and CHX (0 ± 0; <i>p</i> < 0.001) had no growth of <i>E. faecalis</i> colonies compared to blocks of Ca(OH)<sub>2</sub> (10 ± 21) and saline (927 ± 455). All concentrations of NG (0.5 and 1.0 µg) showed effectiveness higher (<i>p</i> < 0.001) than 2% CHX when measured by the zone of inhibition against <i>E. faecalis.</i> CONCLUSION: It may be concluded that NG is effective against growth of <i>E. faecalis</i> and may be used as a promising antimicrobial agent during root canal treatment. However, further studies should be done to investigate the effect of NG against other dental pathogens.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":"103-109"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11750351/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141558425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A Reappraisal of the Accuracy of the Tactile Method for the Detection of the Subgingival Cementoenamel Junction: An In Vivo Study.","authors":"Jaruta Mokhagul, Attawood Lertpimonchai, Lakshman Samaranayake, Orawan Charatkulangkun","doi":"10.1055/s-0044-1786865","DOIUrl":"10.1055/s-0044-1786865","url":null,"abstract":"<p><strong>Objectives: </strong> This article reappraises the accuracy and factors associated with the detection of the cementoenamel junction (CEJ) using the tactile method.</p><p><strong>Materials and methods: </strong> A total of 111 tooth sites of 7 patients scheduled for flap surgery were selected for the study. The CEJ was detected in a blind manner using the conventional tactile method with a standard periodontal probe by a single, trained examiner. A custom-made stent was prepared to standardize the measurements and the distance from a fixed reference point on the stent to the CEJ was measured before (apparent CEJ) and after (real CEJ) opening a gingival flap. To evaluate the effect of local anesthesia (LA) on the measurement error, assessment with and without LA given prior to the measurement was also evaluated. The bone crest-CEJ distance at each site was also recorded in all sites.</p><p><strong>Statistical analysis: </strong> The measurement error of apparent versus real distance, if any, was compared using Cohen's weighted kappa coefficient (WKC) (± 1 mm).</p><p><strong>Results: </strong> A weak WKC (WKC = 0.539) was found between the apparent and real CEJ distance. Higher WKCs were noted at posterior and proximal sites than the anterior and buccal/lingual sites, respectively (0.840 and 0.545 vs. 0.475 and 0.488). A higher confluence of the agreements was noted when CEJ distance was measured in anesthetized sites (WKC = 0.703). Sites without bone loss showed more coronal deviation of CEJ detection, as opposed to apical deviation seen at sites with bone loss.</p><p><strong>Conclusion: </strong> The conventional CEJ detection using the tactile method was relatively imprecise depending on the anatomical location of the tooth and the bone loss at the site of measurement. However, the detection accuracy improved when the sites were anesthetized. In clinical terms, our data, reported here for the first time imply that, in the absence of visual cues, posterior tooth site measurements of periodontal attachment loss were more reliable in comparison to the other sites. The bone crest level also impacted the measurement deviation to some extent, implying that, possible overestimate of clinical attachment loss may occur at sites without bone loss.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":"96-102"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11750324/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140956939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}