European Journal of Dentistry最新文献

{"title":"Moringa oleifera L. Nanosuspension Extract Administration Affects Heat Shock Protein-10 and -70 under Orthodontics Mechanical Force In Vivo.","authors":"Ari Triwardhani, Alida Alida, Ervina Restiwulan Winoto, Adya Pramusita, Nurul Aisyah Rizki Putranti, Kristian Satrio Ariadi, Okso Brillian Pribadi, Amelia Aisyiah Anwar, Alqomariyah Eka Purnamasari, Rifqah Ananda Mappananrang, Putri Cahaya Situmorang, Wibi Riawan, Tengku Natasha Eleena Binti Tengku Ahmad Noor, Albertus Putera Nugraha, Alexander Patera Nugraha","doi":"10.1055/s-0044-1791937","DOIUrl":"10.1055/s-0044-1791937","url":null,"abstract":"<p><strong>Objective: </strong> The mechanical stimulation known as orthodontic mechanical force (OMF) causes biological reactions in orthodontic tooth movement (OTM). Heat shock protein-70 (HSP-70) needs pro-inflammatory cytokines to trigger bone resorption in OTM; nevertheless, heat shock protein-10 (HSP-10), a \"Alarmin\" cytokine, should control these pro-inflammatory cytokines to get the best alveolar bone remodeling (ABR). <i>Moringa oleifera</i> L. nanosuspension extract (MONE) has anti-inflammatory, antioxidant, and ABR-stimulating properties. The aim of the study was to examine <i>in vivo</i> HSP-10 and HSP-70 expressions under OMF following MONE application in Wistar rats (<i>Rattus norvegicus</i>).</p><p><strong>Material and methods: </strong> A total of 36 Wistar rats (<i>R. norvegicus</i>) were split up into eight groups: one for treatment (OMF + MONE) and one for control (OMF + MONE administration for days 1, 7, 14, and 21). By employing nickel-titanium coil springs and using 10 g of light force per millimeter to implant the orthodontic device, the OMF was completed. According to the day of observation, all of the samples were sacrificed. To perform an immunohistochemistry investigation, the premaxilla of the sample was isolated. Tukey's Honest Significant Different (HSD) test (<i>p</i> < 0.05) was performed after an Analysis of Variance (ANOVA) analysis of the data.</p><p><strong>Results: </strong> In both the OMF and MONE groups, HSP-70 peaked on day 14 and began to fall on day 21. HSP-10 peaked on day 21, but along with MONE, it also began to progressively decline on days 14 and 21, with significant differences (<i>p</i> < 0.05).</p><p><strong>Conclusion: </strong> According to immunohistochemistry evidence, postadministration of MONE markedly elevated HSP-10 but lowered HSP-70 expression in the alveolar bone of Wistar rats under OMF.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":"523-530"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12020578/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142946824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Comparison of Esthetic Preferences on Female Skeletal Class II Alterations among Laypeople of Different Facial Profiles.","authors":"Wiwan Tipyanggul, Chidsanu Changsiripun, Niramol Chamnannidiadha","doi":"10.1055/s-0044-1788654","DOIUrl":"10.1055/s-0044-1788654","url":null,"abstract":"<p><strong>Objectives: </strong> This study aimed to investigate the influences of assessors' different personal profiles on the esthetic perception of Class II facial profile corrections and the agreement between profile and silhouette images.</p><p><strong>Materials and methods: </strong> A profile photo of a female with skeletal Class II was digitally altered into three profile and three silhouette images (most pronounced Class II division 1 characteristic, more retruded upper lip position, and more protruded mandibular position). Ninety-six laypeople from three facial profile groups (straight, convex, and concave profiles) chose these images for facial attractiveness. Data were analyzed using an SPSS program. Cohen's kappa coefficient and intraclass correlation coefficients were applied to determine intraparticipant and intra-examiner reliabilities. Chi-square tests were used to test between-group preferences and the relationship of profile preference with other factors. Cohen's kappa coefficient was used to test the agreement in selecting profile and silhouette images (<i>p</i> = 0.05).</p><p><strong>Results: </strong> All groups favored profiles with a protruded mandibular position (11-degree facial contour angle [FCA] and 91-degree nasolabial angle [NLA]). Despite facial profile differences, preference remained consistent (<i>p</i> = 0.649). The convex group showed a stronger inclination toward an untreated-simulating profile (17-degree FCA and 91-degree NLA). Preferences were consistent regardless of sex (<i>p</i> = 0.198) and education (<i>p</i> = 0.105). The percentage of agreement between profile and silhouette images in the total sample was 67.71% (kappa = 0.386). All groups of participants chose the more retruded upper lip position (17-degree FCA and 107-degree NLA) profile in silhouette more than in photograph.</p><p><strong>Conclusion: </strong> All groups preferred a mandibular advancement-simulating profile. Using the photographs or silhouettes to assess the esthetic preference resulted in a similar trend. However, the flatter profile was more preferred in silhouette than in photograph.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":"366-373"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12020620/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141792255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Scientific Research Trend on Guided Tissue Regeneration: A Bibliometric Analysis.","authors":"Abdulkareem Abdullah Alhumaidan, Beenish Fatima Alam, Asim Alsuwaiyan, Eman Ahmed Aljoghaiman, Mohammad Helmi, Saqib Ali","doi":"10.1055/s-0044-1791529","DOIUrl":"10.1055/s-0044-1791529","url":null,"abstract":"<p><strong>Objectives: </strong> Guided tissue regeneration (GTR) is a widely used technique in contemporary dentistry which helps achieve regeneration of periodontal tissues. This study aims to identify leading countries, authors, institutes, journals, scientific publications, and mostly used keyword regarding role of GTR in treatment for periodontal disease using the Scopus database.</p><p><strong>Materials and methods: </strong> A well-curated search through Scopus database for significant literature related to GTR published between 1987 and 2023 was performed. Bibliographical data which comprised of abstracts, title, keywords, references, citations, and other relevant information were composed. The data was analyzed using MS Excel and VOSviewer.</p><p><strong>Results: </strong> Scientific literature on GTR was manually scrutinized, and 308 paper were analyzed using the Scopus database. The first paper on GTR was published in 1987. <i>Journal of Periodontology</i> was identified as the leading journal, while the United States and Italy were the top contributing countries. University of Sienna was the most productive organization. Roberto Pontoriero was identified as the highly cited author. A highly cited scientific paper was published by Pintippa Bunyaratavej in 2001.</p><p><strong>Conclusion: </strong> The present bibliometric study gives useful information related to the total number of scientific articles published from 1987 to 2023. A rising trend of scientific publication was identified which continued followed by a notable decline after 2004. The analysis also recognized the United States and University of Sienna, from Italy as most active country and organizations, while the <i>Journal of Periodontology</i> as the leading journal.</p><p><strong>Clinical relevance: </strong> This study may assist in continuing education and evidence-based practice for clinicians and new researchers by providing knowledge and aiding literature searches in the domain of GTR used in treatment for periodontal conditions.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":"409-419"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12020599/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142686357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocompatibility and Bioactivity Evaluation of Novel Calcium Silicate-Based Sealer: In Vitro Study on Human Dental Pulp Stem Cells.","authors":"Anggraini Margono, Redho Sara Pratiwi, Anggita Dini Nofarina, Dewa Ayu Npa, Ike Dwi Maharti, Romilda Rosseti","doi":"10.1055/s-0045-1802566","DOIUrl":"https://doi.org/10.1055/s-0045-1802566","url":null,"abstract":"<p><strong>Objective: </strong> This article evaluates the biocompatibility and bioactivity of a novel calcium silicate-based sealer by assessing its impact on the viability, mineral deposition, wound closure, and migration activity of human dental pulp stem cells (hDPSCs).</p><p><strong>Material and methods: </strong> AH Plus and AH Plus Bioceramic were pulverized and sterilized according to International Organization for Standardization 10993-5:2009. The hDPSCs were stored raw materials, reaching 80% confluence after passing stem cell marker tests (CD90 98%, CD105 99.7%, CD73 94%, and LinNeg 0.5%) and were at passage 5 to 6 after serum starvation for 24 hours. The study consisted of four groups: AH Plus at concentrations of 1:1 and 1:4, and AH Plus Bioceramic at concentrations of 1:1 and 1:4. Viability was assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay by measuring optical density values, while mineral deposition was evaluated through Alizarin red staining and analyzed with ImageJ software. Migration activity was measured by calculating migration speed and wound closure percentage using a scratch assay at 24 and 72 hours, with results analyzed by ImageJ.</p><p><strong>Statistical analysis: </strong> Viability, migration, and wound closure results were analyzed using one-way analysis of variance. Mineral deposition was analyzed descriptively.</p><p><strong>Results: </strong> There were significant differences in the viability and mineral deposition of hDPSCs between calcium silicate-based sealers and epoxy resin-based sealers. Calcium silicate-based sealers showed higher viability and better mineral deposition. The migration speed rate of hDPSCs at 24 hours and wound closure at 24 and 72 hours were significantly greater with the novel calcium silicate-based sealer compared with the epoxy resin-based sealer.</p><p><strong>Conclusion: </strong> This study suggests that calcium silicate-based sealers offer advantages over traditional epoxy resin-based sealers, demonstrating superior biocompatibility and bioactivity. These properties may lead to improved clinical outcomes, such as faster healing and fewer posttreatment complications. Further research is needed to explore the full potential of these materials in endodontics.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143977562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating a Novel Visualization Device for Improving File Insertion Accuracy During Root Canal Treatment.","authors":"Abubaker Qutieshat, Rajmohan Sivamani Chidambaram, Gurdeep Singh, Samiya Al Ghammari, Ritaj Al Busaidi, Iman Al Sukaiti, Fatima Al Rawas, Mariam Al Balushi, Zahra Al Lawati, Doaa Ahmed, Taif Al Shirawi","doi":"10.1055/s-0045-1806961","DOIUrl":"https://doi.org/10.1055/s-0045-1806961","url":null,"abstract":"<p><p>This study aimed to evaluate whether adopting a horizontal viewpoint, facilitated by a novel digital assistive device, could enhance endodontic file placement accuracy and reduce operator-dependent variability during root canal treatment.A total of 40 modified upper jaw dental stone models, each accommodating a plastic tooth replacing the upper right second molar, were divided into two groups (<i>n</i> = 20 each). The mesiobuccal canal of each tooth was prepared to a standardized working length of 21.0 mm and a working width of size 35. A size 40 file with a stopper preadjusted to 19.0 mm was then inserted. In the first (conventional) group, files were placed without assistance; in the second (device-assisted) group, a horizontal-view digital device was designed, developed, and used to align and insert the file. The second operator, blinded to the device's purpose, performed all insertions under simulated clinical conditions. An intraoral scanner subsequently captured the vertical distance from the stopper's bottom surface to a standardized anatomical landmark. Pairwise comparisons between the two groups were computed using alignment software to account for potential measurement artifacts.Normality in both groups was confirmed via the Shapiro-Wilk test. An independent-samples <i>t</i>-test compared mean vertical distances. Additionally, differences in stopper positioning were calculated for all aligned virtual models in CloudCompare.The device-assisted group exhibited a significantly shorter mean stopper-to-landmark distance (0.425 mm, standard deviation [SD] = 0.225) than the conventional group (0.971 mm, SD = 0.432) (<i>t</i> = -5.014, <i>p</i> = 2.534 × 10^-5). Pairwise analysis highlighted closer apical positioning in the device-assisted group, closely matching pilot study findings that a 26.57° vertical viewing angle can distort perceptions by 0.5 mm. The device's mean intraoral mounting time was 224 s (SD = 35.2), considered negligible over the full treatment duration.Adopting a horizontal perspective with a novel digital assistive device significantly improved file placement accuracy and reduced operator-dependent variability during root canal treatment. Optimizing this device's design and assessing its cost-effectiveness may facilitate broader clinical adoption and further enhance endodontic procedural outcomes.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143980414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Cross-sectional Designs on Torsional Resistance of Endodontic Nickel-Titanium Files: A Finite Element Study.","authors":"Natchaphon Nanthaprathip, Sarita Morakul, Sirawut Hiran-Us, Pairod Singhatanadgid","doi":"10.1055/s-0044-1791785","DOIUrl":"10.1055/s-0044-1791785","url":null,"abstract":"<p><strong>Objectives: </strong> This study aimed to assess the influence of two key design parameters on the torsional resistance of endodontic rotary files: the ratio of the equivalent radius (<i>re</i> ) to the polar moment of inertia (<i>J</i>), or <i>re</i> /<i>J</i> ratio, and the percentage of the inner core area. Understanding these factors can guide the development of files with improved performance during root canal procedures.</p><p><strong>Materials and methods: </strong> Finite element analysis was employed to simulate the behavior of rotary files under torsional loading conditions. This method allowed for the investigation of maximum shear stress across various cross-sections (D₄-D₁₆) of the files. The relationship between the <i>re</i> /<i>J</i> ratio and the maximum shear stress was also evaluated. To assess the impact of cross-sectional design modifications on stress distribution, the study analyzed files with progressively changing configurations.</p><p><strong>Results: </strong> Regions situated outside the inner core circle experienced lower shear stress compared with a circular shaft. Furthermore, a strong linear correlation was observed between the maximum shear stress experienced by the file, the applied torque during operation, and the <i>re</i> /<i>J</i> ratio. Significantly, the study established a connection between the percentage of the inner core area and the torsional resistance of the file. Files with a larger inner core area exhibited a lower coefficient (<i>C</i>) within a newly derived torsional formula. This lower <i>C</i> value directly translated to a reduction in the maximum shear stress experienced by the file. In essence, files with a higher percentage of inner core area demonstrated enhanced torsional resistance, allowing them to withstand higher torsional loads encountered during root canal procedures.</p><p><strong>Conclusion: </strong> This study identified the <i>re</i> /<i>J</i> ratio and the percentage of inner core area as the most critical design factors influencing the torsional resistance of rotary files. Files with a lower <i>re</i> /<i>J</i> ratio and a larger inner core area experienced lower shear stress, resulting in enhanced torsional resistance and potentially reducing the risk of torsional fracture during use. These findings offer valuable insights for both clinicians selecting rotary files and manufacturers designing future iterations, ultimately contributing to improved safety and efficacy during root canal treatments.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":"513-522"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12020593/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142603748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hypoxia-Preconditioned Human Umbilical Cord Mesenchymal Stem Cells Transplantation Ameliorates Inflammation and Bone Regeneration in Peri-Implantitis Rat Model.","authors":"Mefina Kuntjoro, Nike Hendrijantini, Eric Priyo Prasetyo, Bambang Agustono, Guang Hong","doi":"10.1055/s-0044-1791530","DOIUrl":"10.1055/s-0044-1791530","url":null,"abstract":"<p><strong>Objective: </strong> The failure of dental implant treatments is predominantly attributed to peri-implantitis, which entails chronic inflammation within the peri-implant tissue, ultimately leading to tissue degradation. Addressing this condition, human umbilical cord mesenchymal stem cell (hUCMSC) transplantation serves as a regenerative therapy; however, concerns regarding the viability and efficacy of transplanted cells in inflamed regions persist. Hypoxic preconditioning of hUCMSCs has emerged as a potential strategy for augmenting their regenerative and immunomodulatory capacities. This study aimed to evaluate the expression of inflammatory (tumor necrosis factor [TNF]-α) and bone regenerative biomarkers (nuclear factor of activated T-cell [NFATc1], osteocalcin, collagen type I alpha 1 [COL1α1]) within peri-implantitis models subsequent to the transplantation of hypoxia-preconditioned hUCMSCs.</p><p><strong>Materials and methods: </strong> Peri-implantitis models were established through the insertion of implants into the femur bone of 42 Wistar strain Rattus norvegicus, followed by intrasocket injection of lipopolysaccharide. The experimental animals were categorized into three groups (control, normoxia, and hypoxia) and underwent observation on days 14 and 28. The expression levels of TNF-α, NFATc1, COL1α1, and osteocalcin were evaluated using immunohistochemical staining, and the resulting data were subjected to one-way analysis of variance analysis (<i>p</i> < 0.05).</p><p><strong>Results: </strong> Transplantation of hypoxia-preconditioned hUCMSCs significantly ameliorated inflammation and osteoclastogenesis, as evidenced by significant reductions in TNF-α and NFATc1 expression compared with the control group. Furthermore, hypoxic preconditioning of hUCMSCs demonstrated a significant elevation in the expression of osteocalcin and COL1α1 relative to the control group.</p><p><strong>Conclusion: </strong> The transplantation of hypoxia-preconditioned hUCMSCs exhibited a capacity to ameliorate inflammation and enhance bone regenerative processes in peri-implantitis rat models.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":"420-427"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12020601/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142603752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Expression of HIF-1α and VEGF in Radicular Cysts and Periapical Granulomas.","authors":"Mohammed Amjed Alsaegh, Shishir Ram Shetty, Okba Mahmoud, Sudhir Rama Varma, Alaa Muayad Altaie, Surendra Singh Rawat","doi":"10.1055/s-0044-1795078","DOIUrl":"10.1055/s-0044-1795078","url":null,"abstract":"<p><strong>Objectives: </strong> This study aimed to evaluate the expression levels of hypoxia-inducible factor 1-α (HIF-1α) and vascular endothelial growth factor (VEGF) in radicular cysts and periapical granulomas, thereby contributing to the understanding of their potential significance in the differential diagnosis and treatment of these lesions.</p><p><strong>Materials and methods: </strong> In the present cross-sectional study, 51 samples of periapical lesions were included. Of them, 24 samples were radicular cysts, and 27 samples were periapical granulomas. Samples were immunohistochemically analyzed for HIF-1α and VEGF proteins expression. Chi-square tests and Spearman's rank correlation coefficient tests were used to detect differences and correlations among the parameters, respectively.</p><p><strong>Results: </strong> In radicular cysts, HIF-1α expression was absent in 1 (4.2%), weak in 5 (20.8%), mild in 7 (29.2%), and strong in 11 (45.8%) cases, while VEGF expression was absent in 1 (4.2%), weak in 6 (25.0%), mild in 9 (37.5%), and strong in 8 (33.3%) of the cases; nevertheless, in periapical granulomas, HIF-1α expression was absent in 8 (29.6%), weak in 6 (22.2%), mild in 9 (33.3%), and strong in 4 (14.8%) of the cases, whereas VEGF expression was absent in 4 (14.8%), weak in 16 (59.3%), mild in 4 (14.8%), and strong in 3 (11.1%) of the cases. Chi-square test revealed a significant difference in the expression of HIF-1α and VEGF between radicular cysts and periapical granuloma (chi-square test = 8.906, <i>p</i> = 0.031; chi-square test = 10.401, <i>p</i> = 0.015, respectively). Spearman's correlation test showed a significant correlation between HIF-1α and VEGF in the total samples of both radicular cysts and periapical granulomas (rho = 0.385, <i>p</i> = 0.005).</p><p><strong>Conclusion: </strong> There is high expression of both HIF-1α and VEGF throughout the odontogenic epithelium and connective tissue of the radicular cyst and periapical granuloma. Both HIF-1α and VEGF are more highly expressed in radicular cysts than in periapical granulomas. These findings may aid in the diagnosis and management of suspected periapical lesions, suggesting that radicular cysts exhibit more advanced hypoxic conditions and associated pathways compared with periapical granulomas.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":"531-539"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12020581/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142806575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prediabetes Associates with Matrix Metalloproteinase-8 Activation and Contributes to the Rapid Destruction of Periodontal Tissues.","authors":"Kehinde Adesola Umeizudike, Nur Rahman Ahmad Seno Aji, Katariina Niskanen, Iina Rantala, Dimitra Sakellari, Andreas Grigoriadis, Tommi Pätilä, Shipra Gupta, Timo Sorsa, Ismo T Räisänen","doi":"10.1055/s-0044-1788797","DOIUrl":"10.1055/s-0044-1788797","url":null,"abstract":"<p><strong>Objective: </strong> The aim of this cross-sectional study was to investigate the relationship between periodontitis, potential periodontitis oral fluid biomarkers, and prediabetes.</p><p><strong>Materials and methods: </strong> This study included 150 Greek adults aged 25 to 78 years who were tested with an Hemoglobin A1C (HBA1c) diagnostic system, an active-matrix metalloproteinase-8 (aMMP-8) point-of-care (PoC) test, and several salivary biomarkers enzyme-linked immunosorbent assay tests and gelatin zymography. A full-mouth clinical examination was performed to assess their periodontal and oral health status.</p><p><strong>Statistical analysis: </strong> The Kruskal-Wallis test was used to determine the statistically significant difference in the levels of periodontal oral fluid biomarkers between the different periodontitis stages, periodontitis grades, and the stages and grades of periodontitis combined. Spearman's rank correlation was performed to assess the strength and direction of the association between aMMP-8 and HbA1c levels (<5.7 and ≥5.7%) and with the other oral fluid biomarkers among patients with severe periodontitis. A two-sided <i>p</i>-value below 0.05 was considered statistically significant in this study.</p><p><strong>Results: </strong> aMMP-8, but not total MMP-8 or other biomarkers, associated significantly with the stage and grade of periodontitis combined (<i>p</i> < 0.001, Kruskal-Wallis test). Among stage III grade C periodontitis patients, aMMP-8 levels were significantly positively correlated with prediabetes (Spearman's rho = 0.646, <i>p</i> = 0.044), total MMP-8 (rho = 0.636, <i>p</i> = 0.048), PMN Elastase (rho = 0.729, <i>p</i> = 0.017), total MMP-9 (rho = 0.721, <i>p</i> = 0.019), and total MMP-8/TIMP-1 molar ratio (rho = 0.879, <i>p</i> < 0.001).</p><p><strong>Conclusion: </strong> Prediabetic disease development can upregulate MMP-8 expression (total MMP-8) in rapidly progressing, severe periodontitis, where MMP-8 latent species are further activated into their active forms (aMMP-8). Simultaneously, several proinflammatory biomarker levels are elevated in this tissue-destructive biomarker cascade. This development is easily detectable online/in real-time within 5 minutes by aMMP-8 PoC testing at the dentist's office.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":"305-314"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12020604/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142364881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Particulate Matter 2.5 on Primary Gingival Keratinocyte and Human Gingival Fibroblast Cell Lines.","authors":"Supaporn Mala, Supranee Buranapraditkun, Kanidta Sooklert, Amornpun Sereemaspun, Puangwan Lapthanasupkul, Dulyapong Rungraungrayabkul, Nakarin Kitkumthorn","doi":"10.1055/s-0044-1789269","DOIUrl":"10.1055/s-0044-1789269","url":null,"abstract":"<p><strong>Objective: </strong> Particulate matter 2.5 (PM2.5), an important air pollution particle, has been previously studied for its effects on various normal and cancer tissues. However, research on the impact of PM2.5, specifically on normal cavity tissue, is still limited. This study aimed to assess the effects of PM2.5 on cell vitality, cell cycle, and apoptosis in PGK (normal oral keratinocyte) and HGF (human gingival fibroblast) cell lines.</p><p><strong>Materials and methods: </strong> The effect of PM2.5 was examined through cell vitality using the Cell Counting Kit-8 (CCK8) assay, while cell cycle and apoptosis were determined via flow cytometry. Cells incubated with 0.05% dimethyl sulfoxide were used as the negative control.</p><p><strong>Results: </strong> In a concentration-dependent manner, PM2.5 inhibited the proliferation of HGF and PGK cells. The half-maximal inhibitory concentration (IC50) of PM2.5 after 24 hours of incubation was 400 ng/µL for HGF cells and 100 ng/µL for PGK cells. This particulate matter arrested the cell cycles of both HGF and PGK cells at the G0/G1 phase. Additionally, PM2.5 was found to trigger apoptosis in both HGF and PGK cell lines and also cause necrosis in the PGK cell line at higher concentrations.</p><p><strong>Statistical analysis: </strong> Kruskal-Wallis tests were employed to evaluate all quantitative data.</p><p><strong>Conclusion: </strong> The findings indicated that PM2.5 decreases cell viability, halts cell cycle progression, and triggers apoptosis in normal oral cavity cell lines. Therefore, it is advisable to avoid PM2.5 exposure in order to mitigate potential health risks. To understand PM2.5-induced oral cellular damage, more research is needed.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":"464-471"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12020600/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142921325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}