Adilson Bazachi, Nelson G. C. Astrath, Elton L. Savi, Luis C. Malacarne, Mauro L. Baesso, Alex S. Torquato, Angela C. Rodrigues, Gustavo V. B. Lukasievicz, Leandro S. Herculano
{"title":"Spectroscopic and chemometric analysis and oil stability index characterization of thermo-oxidized edible vegetable oils","authors":"Adilson Bazachi, Nelson G. C. Astrath, Elton L. Savi, Luis C. Malacarne, Mauro L. Baesso, Alex S. Torquato, Angela C. Rodrigues, Gustavo V. B. Lukasievicz, Leandro S. Herculano","doi":"10.1002/ejlt.202300204","DOIUrl":"10.1002/ejlt.202300204","url":null,"abstract":"<p>Edible vegetable oils are sources of polyunsaturated fatty acids, necessary for a balanced diet capable of providing elements that act on the energetic, structural, and hormonal composition of humans. The growing consumption of these foods has encouraged the search for techniques capable of characterizing their compositions and transformations when subjected to industrial processes or during domestic use. We propose to analyze the transformations undergone by edible vegetable oils originating from different plants due to thermal oxidation. For this, dynamic viscosity, oxidative stability index, fatty acid profile, and infrared spectra determined before and after being subjected to thermal oxidation. The results from infrared spectroscopy were improved through Principal Component Analysis (PCA). Among other results, it was possible to establish correlations between the FTIR spectra, dynamic viscosity, and the profile of fatty acids, allowing the prediction of the concentration of polyunsaturated fatty acids (PUFA) after thermal oxidation by measuring the spectrum of samples before the thermal oxidation process. Furthermore, it is observed that the dynamic viscosity is strongly altered by thermal oxidation, which is directly related to the decrease in PUFA content. The results obtained can be used to predict quality factors of edible vegetable oils, helping to choose the right type of oil for each industrial or domestic process.</p><p><i>Practical Applications</i>: This research holds significant practical implications, particularly in detecting adulteration and fraud of edible vegetable oils. The developed method uses physicochemical properties and infrared spectroscopy with principal component analysis to characterize oils and to determine the oil stability index.</p>","PeriodicalId":11988,"journal":{"name":"European Journal of Lipid Science and Technology","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141744079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Cover Picture: Eur. J. Lipid Sci. Technol. 7/2024","authors":"","doi":"10.1002/ejlt.202470053","DOIUrl":"10.1002/ejlt.202470053","url":null,"abstract":"<p>Simplified cholesterol backbone with images of a random spectral trace, a palm leaf (Rainer Sturm/pixelio.de), fluorescently stained lipid droplets (red) and associated proteins (green) in a T3T-L1 adipocyte (Johanna Spandl, University of Bonn, Germany), and fish (Vera/pixelio.de).\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":11988,"journal":{"name":"European Journal of Lipid Science and Technology","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ejlt.202470053","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141547331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Juliette Wind, Pierre Villeneuve, Mignon Prince Exaucé Taty, Maria Cruz Figueroa-Espinoza, Bruno Baréa, Rémi Pradelles, Erwann Durand
{"title":"Improving the triphenylphosphine/triphenylphosphine oxide (TPP/TPPO)-based method for the absolute and accurate quantification by FTIR-ATR of hydroperoxides in oils or lipid extracts","authors":"Juliette Wind, Pierre Villeneuve, Mignon Prince Exaucé Taty, Maria Cruz Figueroa-Espinoza, Bruno Baréa, Rémi Pradelles, Erwann Durand","doi":"10.1002/ejlt.202400030","DOIUrl":"10.1002/ejlt.202400030","url":null,"abstract":"<p>Research on natural sources of polyunsaturated fatty acids (PUFAs) for both food and nutraceutical prospects has significantly grown in recent years. Some plant oils and lipid extracts contain carotenoids, xanthophylls, sterols, and/or phenolic compounds that can provoke a lipid hydroperoxides (LOOHs) overestimation when quantifying them using nonselective colorimetric assays. Herein, we have optimized a mid-infrared method using Fourier-transform infrared spectroscopy coupled with attenuated total reflectance (ATR) to quantify LOOHs in oils or lipid extracts. This method is based on the conversion of triphenylphosphine (TPP) to triphenylphosphine oxide (TPPO) in the presence of hydroperoxides, with a direct assessment of TPPO levels following the formation of an oil film on the ATR crystal's surface, allowing for a low detection limit of 0.5 mmol of LOOH kg<sup>−1</sup>. The concentration of oil and TPP, as well as the reaction time, were optimized. It was demonstrated that the presence of pigments, unsaponifiable compounds, phenolics, and/or PUFAs on oils, do not disrupt the analysis. Furthermore, the stoichiometry of the TPP/LOOH reaction was examined, confirming the reliability of the method in detecting various forms of hydroperoxides. An accelerated oxidation study was carried out and the hydroperoxide contents measured using TPP/TPPO were found to be comparable to those obtained using the ferric thiocyanate method. Our method offers a fast, simple, robust, and sensitive approach to accurately quantify hydroperoxides, regardless of the chemical composition of oil or lipid extracts.</p><p><i>Practical Application</i>: The hydroperoxide assay method outlined in this study allows for the rapid and straightforward detection of hydroperoxides in pure oil matrices. The method's elevated sensitivity facilitates the early identification of oxidation indicators in oils, especially those with significant carotenoid or xanthophyll contents since these compounds may affect the results when using methods based on colorimetric quantification of hydroperoxides. This accurate, precise, and reproducible approach requires only small quantities of test samples and chemical products, making it well suited to routine application in laboratories and industrial environments.</p>","PeriodicalId":11988,"journal":{"name":"European Journal of Lipid Science and Technology","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ejlt.202400030","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141547330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Editorial Board: Eur. J. Lipid Sci. Technol. 7/2024","authors":"","doi":"10.1002/ejlt.202470054","DOIUrl":"10.1002/ejlt.202470054","url":null,"abstract":"","PeriodicalId":11988,"journal":{"name":"European Journal of Lipid Science and Technology","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ejlt.202470054","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141552500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Contents: Eur. J. Lipid Sci. Technol. 7/2024","authors":"","doi":"10.1002/ejlt.202470055","DOIUrl":"10.1002/ejlt.202470055","url":null,"abstract":"","PeriodicalId":11988,"journal":{"name":"European Journal of Lipid Science and Technology","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ejlt.202470055","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141552499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yalda Khodadadi Jokar, Mohammad Goli, Mojtaba Nasr Esfahani, Mohammad Fazel, Afsaneh Najarian
{"title":"Microencapsulation of fish oil rich in EPA and DHA using mixture of Arabic gum and Persian gum through spray-drying technique","authors":"Yalda Khodadadi Jokar, Mohammad Goli, Mojtaba Nasr Esfahani, Mohammad Fazel, Afsaneh Najarian","doi":"10.1002/ejlt.202300239","DOIUrl":"10.1002/ejlt.202300239","url":null,"abstract":"<p>The microencapsulation of fish oil by the spray-drying technique was conducted using Arabic gum (AG) and Persian gum (PG) as wall materials. AG-to-PG ratios, including 29:1, 28:2, 27:3, 26:4, and 25:5 (%w/w), wall-to-oil ratios, including 5:1, 4:1, 3:1, 2;1, and 1:1, drying temperature (180, 190, 200, 210, and 220°C), and feed flow rate at high and low states were optimized using response surface methodology. Microencapsulation efficiency (MEE), moisture content (MC), peroxide value (PV), and particle size (PS) were determined. Results showed that the highest MEE and the lowest MC, PV, and PS were attained when 26:4, 4:1, 210°C, and high speed were considered, respectively. At this point, the MEE, MC, PV, and PS were 79.49%, 3.39%, 10.98 meq O<sub>2</sub>/kg oil, and 39.05 µm, respectively. The microstructure of optimum microencapsulated powder exhibited no observable cracks, fissures, or pores while having a typical spherical and smooth surface. Microencapsulation of fish oil using a mixture of AG and PG showed higher oxidative stability associated with high MEE, low MC, and low PV at the final product. Moreover, the optimized emulsion formulation and drying conditions increased the storage stability.</p>","PeriodicalId":11988,"journal":{"name":"European Journal of Lipid Science and Technology","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141511994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yasmine M. Ben Amar, Angela G. Potortì, Ambrogina Albergamo, Federica Litrenta, Rossana Rando, Lahkim B. Mouad, Jamal Brigui, Noureddine Chouaibi, Giuseppa Di Bella
{"title":"Study of the lipid fraction of Moroccan and Italian carobs (Ceratonia siliqua L.)","authors":"Yasmine M. Ben Amar, Angela G. Potortì, Ambrogina Albergamo, Federica Litrenta, Rossana Rando, Lahkim B. Mouad, Jamal Brigui, Noureddine Chouaibi, Giuseppa Di Bella","doi":"10.1002/ejlt.202400036","DOIUrl":"10.1002/ejlt.202400036","url":null,"abstract":"<p>Carob (<i>Ceratonia siliqua</i> L.) is a characteristic fruit from the Mediterranean area, composed of a hard pulp and numerous seeds that have been unfairly undervalued and underutilized over time. To enhance the Mediterranean's biodiversity and support food production from sustainable resources, the carob has recently received attention for its nutritional value, and it has advantageously served as a healthy ingredient of many food products. Hence, in this study, the composition of the Mediterranean carob was further deepened by investigating its lipid fraction on a dry weight basis in relation to the fruit part (i.e., pulp and seeds) and the geographical origin (i.e., different Moroccan and Italian provinces). On average, lipids of pulp and seeds amounted to 1.71% and 2.46%, respectively. Predominant fatty acids were palmitic, oleic, and linoleic acids. Palmitic and oleic acids were higher in pulp (20.21%–26.50% and 37.63%–44.49%) than seeds (13.93%–17.26% and 31.92%–36.66%), whereas linoleic acid showed an opposite accumulation trend (pulp: 8.32%–15.46%, seeds: 38.19%–43.45%). Among sterols, β-sitosterol (57.49%–72.47%), Δ-5-avenasterol (10.26%–21.27%), and stigmasterol (3.97%–8.11%) accounted for more than 70% of total sterols of pulp, whereas β-sitosterol alone represented more than 70% of sterols in seeds. Considering tocopherols, γ-tocopherol was the most abundant isomer both in pulp (10.37–22.78 mg/100 g) and seeds (4.95–22.86 mg/100 g). Carob also resulted in a good source of squalene, which on average was 2.17 mg kg<sup>−1</sup> in pulp and 19.07 mg kg<sup>−1</sup> in seeds. A principal component analysis pointed out that the study of the lipid fraction of carob can help in discriminating samples based on their Moroccan or Italian origin.</p><p><i>Practical Applications</i>: The study provides further insights into the still-little-explored lipid fraction of Mediterranean carob and fills a relevant gap in the literature by contributing to a more exhaustive framework of the nutritional and functional value of this fruit. In a wider perspective, it can be of practical help in the formulation of carob-based products and in the establishment of a more informed market of carob and carob-based products.</p>","PeriodicalId":11988,"journal":{"name":"European Journal of Lipid Science and Technology","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141511925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Design and optimization of metformin solid lipid microparticles for topical application","authors":"Daya Mancer, Farid Agouillal, Kamel Daoud","doi":"10.1002/ejlt.202300221","DOIUrl":"10.1002/ejlt.202300221","url":null,"abstract":"<p>This study aimed to improve metformin skin administration by creating solid lipid microparticles (SLMs). To obtain optimal metformin delivery, SLMs were created using a double emulsion hot homogenization technique with a rotor-stator. The effects of the two surfactants and homogenization time on particle characteristics and performance were studied using Response Surface Methodology (RSM). Tween 60 concentration had the most significant effect on particle size. The simple effects of the studied factors did not significantly affect encapsulation efficiency. However, the interactions between these parameters influenced this response. Moreover, the particle size was affected more by the surfactant concentration. After optimizing the three factors, the results showed an optimum encapsulation efficiency of 82% and a particle size of 2 µm with a desirability of 0.915. The topical drug release profile of lipid microparticle suspensions is characterized by an early burst, followed by sustained drug release. The release of metformin from solid lipid particles followed the Higuchi release model, whereas it followed the Weibull model for release from the gel formulation. Based on the in vitro drug release results, we can conclude that the particles containing the drug are in the shape of a matrix.</p><p><i>Practical Applications</i>: The SLM formulation produces a film on the cutaneous surface, retaining the active component in the skin's superficial layer. Furthermore, owing to their micron size, SLM increases the contact surface of the encapsulated drug with the stratum corneum, which might improve cutaneous delivery and provide sustained release of the drug.</p>","PeriodicalId":11988,"journal":{"name":"European Journal of Lipid Science and Technology","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141511926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Asma El Kaourat, Said El Harkaoui, Badr Eddine Kartah, Hasnae Choukri, Dominique Guillaume, Bertrand Matthäus, Hanae El Monfalouti
{"title":"Assessment of lipid profile and antioxidant activity of pulp, shell, and kernel of Moroccan Balanites aegyptiaca fruit","authors":"Asma El Kaourat, Said El Harkaoui, Badr Eddine Kartah, Hasnae Choukri, Dominique Guillaume, Bertrand Matthäus, Hanae El Monfalouti","doi":"10.1002/ejlt.202400013","DOIUrl":"10.1002/ejlt.202400013","url":null,"abstract":"<p>The <i>Balanites aegyptiaca</i> tree is widely used in many countries where it occurs. However, in Morocco its nutritional properties and chemical composition have not been extensively studied. This study aims to investigate its potential health benefits, focusing on bioactive compounds found in the fruit, including the pulp, shell and kernel. The fatty acid composition, phytosterol content, triacylglycerols and tocopherol levels of the lipid fraction extracted from different parts of <i>Balanites</i> fruit were analyzed using chromatographic techniques. Additionally, the antioxidant activity was assessed through the DPPH, ABTS, and FRAP assays. Results showed that the pulp, shell and kernel are rich in oleic acid, with percentages of 47.2%, 45.9%, and 41.9%, respectively. The linoleic acid content was consistent across all three parts. The shell had the highest phytosterols content, with β-sitosterol being the most abundant in all three parts. The analysis revealed some variability in the identification of tocopherols, with the kernel containing the highest content of tocopherols, mainly α-Tocopherol at 399 mg kg<sup>−1</sup>. The fruit, in its entirety, exhibits antioxidant activity as evaluated by three different methods: DPPH, ABTS, and FRAP. Overall, the study demonstrates that the fruit of <i>B. aegyptiaca</i> growing in Morocco possesses properties that could be utilized in the food and medical industries.</p><p><i>Practical Application</i>: The fruit parts of the Balanites tree (<i>Balanites aegyptiaca</i>) are rich in fatty acids, tocochromanols, and phytosterols, exhibiting significant antioxidant capacity. This study reveals the potential of the bioactive compounds of the fruit, which offers substantial practical applications as a natural source of antioxidants in food formulations and nutraceuticals. By leveraging the bioactive compounds identified in this study, researchers and industries can investigate the development of functional food and cosmeceuticals formulations to enhance human health and well-being.</p>","PeriodicalId":11988,"journal":{"name":"European Journal of Lipid Science and Technology","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141530409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Raoudha Ben Ameur, Basma Hadjkacem, Mohamed Ayadi, Ben Amor Ikram, Amira Feki, Jalel Gargouri, Ali Gargouri, Noureddine Allouche
{"title":"Phytochemical profile of Tunisian Pistacia lentiscus fruits oil: Antioxidant, antiplatelet, and cytotoxic activities assessment","authors":"Raoudha Ben Ameur, Basma Hadjkacem, Mohamed Ayadi, Ben Amor Ikram, Amira Feki, Jalel Gargouri, Ali Gargouri, Noureddine Allouche","doi":"10.1002/ejlt.202300274","DOIUrl":"10.1002/ejlt.202300274","url":null,"abstract":"<p>This study aims to comprehensively examine the chemical composition, physicochemical characterization, as well as antioxidant and antiplatelet activities of <i>Pistacia lentiscus</i> fruit oil (PLFO). Our results showed that the prominent class of fatty acids was represented by monounsaturated fatty acids (42.73%), followed by saturated fatty acids (33.99%) and polyunsaturated fatty acids (23.13%). The different ratios of fatty acids were determined (PUFA/MUFA = 0.54, PUFA/SAFA = 0.68 and MUFA/SAFA = 1.25). The obtained results related to saturated and polysaturated fatty acids ratios were in line with the recommendations of the World Health Organization (WHO). The principal fatty acid (FA) consisted in oleic acid (41.32%), followed by linoleic acid (23.08%). A comparison was made with Moroccan and Algerian PLFO showing that Tunisian PLFO is the richest in linoleic acid. The main tentatively identified triacylglycerols, which are in monounsaturated and polyunsaturated forms, were SLL+PLO (19.73%), OOL+LnPP (13.12%) and POO (10.57%). The primary sterol identified in PLFO was <i>β</i>-sitosterol (80.19%). However, tocopherols were quantified at a content of 1249.16 mg g<sup>–1</sup> of oil. The effect on platelet aggregation was evaluated on human platelet-rich plasma treated with various PLFO concentrations, dissolved in ethanol (named POE) and then induced by ADP, collagen, and arachidonic acid (AA). The outcome revealed that 8 µL mL<sup>–1</sup> of POE strongly inhibited ADP-induced platelet aggregation in humans. Additionally, the expression of CD63 and P-selectin as markers of platelet secretion, and <i> α</i>ΙΙ<i>β</i>3 integrin activation were assessed by flow cytometry. It has been found that PLFO significantly reduced platelet activation as well as alpha and dense granule secretion. Moreover, the cytotoxicity assay on normal HEK-293 cells, the hemolysis test and the platelet viability confirmed that PLFO is a safe substance. These findings are valuable for assessing the nutritional composition of PLFO within a preventive and/or therapeutic framework in a clinical context.</p><p><i>Practical applications</i>: PLFO is mainly used to alleviate problems related to poor blood circulation, especially for women but it is also employed to treat problems such as heavy legs and varicose veins. Furthermore, PLFO would prevent certain diseases such as phlebitis, and alleviates various disorders, including cardiovascular problems.</p>","PeriodicalId":11988,"journal":{"name":"European Journal of Lipid Science and Technology","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ejlt.202300274","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141511928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}