Enzyme最新文献_第9页

Control of translation of masked mRNAs in clam oocytes. 蛤卵母细胞中隐藏mrna翻译的控制。

Enzyme Pub Date : 1990-01-01 DOI: 10.1159/000468751

N Standart, T Hunt

引用次数: 13

Age-dependent excretion of alanine aminopeptidase, alkaline phosphatase, gamma-glutamyltransferase and N-acetyl-beta-D-glucosaminidase in human urine. 人尿中丙氨酸肽酶、碱性磷酸酶、γ -谷氨酰转移酶和n -乙酰- β -d -氨基葡萄糖酶的年龄依赖性排泄。

Enzyme Pub Date : 1990-01-01 DOI: 10.1159/000468700

K Jung, A Hempel, K D Grützmann, R D Hempel, G Schreiber

引用次数: 32

Regulation of viral and cellular RNA turnover in cells infected by eukaryotic viruses including HIV-1. 包括HIV-1在内的真核病毒感染的细胞中病毒和细胞RNA周转的调控。

Enzyme Pub Date : 1990-01-01 DOI: 10.1159/000468769

M G Katze, M B Agy

引用次数: 16

Ornithine transcarbamylase deficiency in male adolescence and adulthood. 男性青春期和成年期鸟氨酸转氨基甲酰基酶缺乏症。

Enzyme Pub Date : 1990-01-01 DOI: 10.1159/000468724

M Yoshino, J Nishiyori, F Yamashita, R Kumashiro, H Abe, K Tanikawa, T Ohno, K Nakao, N Kaku, H Fukushima

引用次数: 33

Preferential inhibition of lysosomal beta-mannosidase by sucrose. 蔗糖对溶酶体-甘露糖苷酶的优先抑制作用。

Enzyme Pub Date : 1990-01-01 DOI: 10.1159/000468720

N R McCabe, W Biliter, G Dawson

{"title":"Preferential inhibition of lysosomal beta-mannosidase by sucrose.","authors":"N R McCabe, W Biliter, G Dawson","doi":"10.1159/000468720","DOIUrl":"https://doi.org/10.1159/000468720","url":null,"abstract":"The lysosomal storage disease beta-mannosidosis, described in both goats and humans, can be detected by measuring a deficiency in hydrolysis of the fluorogenic substrate 4-methylumbelliferyl-beta-D-mannoside. An inhibitor of guinea pig beta-mannosidase (beta-man) activity was detected when tissue was homogenized in phosphate-buffered-saline (pH 7.4) containing 0.25 mol/l sucrose. The existence of such an inhibitor was apparent when the enzyme was immunoprecipitated from tissue using a specific beta-man polyclonal antibody. There was up to a threefold increase in activity in the immunoprecipitated enzyme (antibody-enzyme complex) compared to the activity of the nonimmunoprecipitated enzyme. An extensive study was therefore undertaken to determine the nature and specificity of this inhibitor by analyzing the effect of a range of metal ions and sugars on beta-man activity compared to other lysosomal hydrolase activities. Although ferrous, ferric, cobalt, and manganese ions were highly inhibitory to beta-man, they also inhibited other lysosomal hydrolases to a similar extent. Likewise, mannose inhibited both alpha- and beta-man activities equally. The only compound to specifically inhibit beta-man in a manner similar to that observed in the tissue homogenate was glucosyl(beta, 2)fructofuranoside (sucrose). This is an important finding in that tissue samples are commonly prepared in buffers containing sucrose and this could lead to a wrong diagnosis of beta-man deficiency. In order to determine if the absence of an activator factor or alternatively the presence of a specific inhibitor was a contributing factor in the lack of beta-man activity in cultured fibroblasts from affected humans and goats, mixing studies with normal and affected cell extracts were performed but no restoration or inhibition of beta-man activity was found.","PeriodicalId":11933,"journal":{"name":"Enzyme","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000468720","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13250474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Translational regulation of the expression of ribosomal protein genes in Xenopus laevis. 非洲爪蟾核糖体蛋白基因表达的翻译调控。

Enzyme Pub Date : 1990-01-01 DOI: 10.1159/000468750

F Amaldi, P Pierandrei-Amaldi

引用次数: 30

cis-acting determinants of c-myc mRNA stability. c-myc mRNA稳定性的顺式决定因素。

Enzyme Pub Date : 1990-01-01 DOI: 10.1159/000468755

M D Cole, S E Mango

引用次数: 19

Translational control in influenza virus-infected cells. 流感病毒感染细胞的翻译控制。

Enzyme Pub Date : 1990-01-01 DOI: 10.1159/000468764

M G Katze, R M Krug

引用次数: 0

Inflammatory bowel disease and serum beta-N-acetylhexosaminidase. 炎症性肠病和血清β - n -乙酰己糖氨酸酶。

Enzyme Pub Date : 1990-01-01 DOI: 10.1159/000468721

E Scapa, M Neuman, J Eshchar

引用次数: 3

Cap-independent translation of picornavirus RNAs: structure and function of the internal ribosomal entry site. 小核糖核酸不依赖于帽的翻译:内部核糖体进入位点的结构和功能。

Enzyme Pub Date : 1990-01-01 DOI: 10.1159/000468766

S K Jang, T V Pestova, C U Hellen, G W Witherell, E Wimmer

{"title":"Cap-independent translation of picornavirus RNAs: structure and function of the internal ribosomal entry site.","authors":"S K Jang, T V Pestova, C U Hellen, G W Witherell, E Wimmer","doi":"10.1159/000468766","DOIUrl":"https://doi.org/10.1159/000468766","url":null,"abstract":"Picornaviruses are mammalian plus-strand RNA viruses whose genomes serve as mRNA. A study of the structure and function of these viral mRNAs has revealed differences among them in events leading to the initiation of protein synthesis. A large segment of the 5' nontranslated region, approximately 400 nucleotides in length, promotes 'internal' entry of ribosomes independent of the non-capped 5' end of the mRNA. This segment, which we have called the internal ribosome entry site (IRES), maps approximately 200 nt down-stream from the 5' end and is highly structured. IRES elements of different picornaviruses, although functionally similar in vitro and in vivo, are not identical in sequence or structure. However, IRES elements of the genera entero- and rhinoviruses, on the one hand, and cardio- and aphthoviruses, on the other hand, reveal similarities corresponding to phylogenetic kinship. All IRES elements contain a conserved Yn-Xm-AUG unit (Y, pyrimidine; X, nucleotide) which appears essential for IRES function. The IRES elements of cardio-, entero- and aphthoviruses bind a cellular protein, p57. In the case of cardioviruses, the interaction between a specific stem-loop of the IREs is essential for translation in vitro. The IRES elements of entero- and cardioviruses also bind the cellular protein, p52, but the significance of this interaction remains to be shown. The function of p57 or p52 in cellular metabolism is unknown. Since picornaviral IRES elements function in vivo in the absence of any viral gene products, we speculate that IRES-like elements may also occur in specific cellular mRNAs releasing them from cap-dependent translation. IRES elements are useful tools in the construction of high yield expression vectors, or for tagging cellular genetic elements.","PeriodicalId":11933,"journal":{"name":"Enzyme","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000468766","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13126173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 210