Enzyme Pub Date : 1990-01-01 DOI: 10.1159/000468699

S K Das, B L Fanburg

引用次数: 2

In vitro regulation of rat liver L-threonine deaminase by different effectors. 不同效应物对大鼠肝脏l -苏氨酸脱氨酶的体外调节作用。

Enzyme Pub Date : 1990-01-01 DOI: 10.1159/000468718

R Pagani, R Leoncini, L Terzuoli, R Guerranti, E Marinello

引用次数: 2

Overview: phosphorylation and translation control. 概述:磷酸化和翻译控制。

Enzyme Pub Date : 1990-01-01 DOI: 10.1159/000468744

J W Hershey

引用次数: 31

Translationally regulated genes in higher eukaryotes. 高等真核生物中翻译调控的基因。

Enzyme Pub Date : 1990-01-01

引用次数: 0

Maternal mRNA expression in early development: regulation at the 3' end. 母体mRNA在发育早期的表达:在3'端调控。

Enzyme Pub Date : 1990-01-01 DOI: 10.1159/000468753

J D Richter, J Paris, L L McGrew

引用次数: 14

delta-Aminolevulinic acid dehydratase: is there a form unique to renal cortex? 氨基乙酰丙酸脱水酶:是否有肾皮质独有的形式?

Enzyme Pub Date : 1990-01-01 DOI: 10.1159/000468701

K S Roth, P D Spencer, L C Moses, B E Carter

{"title":"delta-Aminolevulinic acid dehydratase: is there a form unique to renal cortex?","authors":"K S Roth, P D Spencer, L C Moses, B E Carter","doi":"10.1159/000468701","DOIUrl":"https://doi.org/10.1159/000468701","url":null,"abstract":"Succinylacetone (SA) is known to be a potent inhibitor of delta-aminolevulinic acid dehydratase (ALAD) in the liver. We have examined the effects of SA on the rat renal cortical enzyme, our observations indicating very different behaviour of renal versus hepatic ALAD with SA treatment. While the temperature response of ALAD in both tissues was similar, addition of 4 mmol/l SA inhibited liver ALAD at 37 and 55 degrees C and enhanced renal ALAD activity 2- to 3-fold at each temperature. This increase in renal ALAD was progressive with SA concentrations form 1 to 10 mmol/l. A pH titration curve for both liver and kidney ALAD showed the hepatic enzyme to have a single pH optimum, while the renal enzyme had two, each of which was distinct from that in liver. Kinetic studies with and without 4 mmol/l SA over a 50-fold ALA concentration range indicated SA-induced enhancement of renal ALAD over the entire range at both pH optima. Using 14C-labelled ALA, we have confirmed these observations made on the basis of a colorimetric assay for PBG, the enzyme product. We conclude that renal ALAD may be a different molecular species from the liver enzyme. Further studies may clarify the significance of these observations to renal heme synthesis.","PeriodicalId":11933,"journal":{"name":"Enzyme","volume":"43 1","pages":"17-25"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000468701","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13510344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Effect of alcohol on lipoprotein metabolism. II. Lipolytic activities and mixed function oxidases. 酒精对脂蛋白代谢的影响。2脂溶活性和混合功能氧化酶。

Enzyme Pub Date : 1990-01-01 DOI: 10.1159/000468704

J G Parkes, W Auerbach, D M Goldberg

{"title":"Effect of alcohol on lipoprotein metabolism. II. Lipolytic activities and mixed function oxidases.","authors":"J G Parkes, W Auerbach, D M Goldberg","doi":"10.1159/000468704","DOIUrl":"https://doi.org/10.1159/000468704","url":null,"abstract":"The mechanism by which alcohol increases plasma total high density lipoproteins (HDLs) and HDL-cholesterol is unknown, but it may involve modulation of the lipolytic enzymes, hepatic triglyceride lipase (HTGL) and/or lipoprotein lipase (LPL) in hepatic and extrahepatic tissues. The modulation of HDL metabolism by alcohol may also be related to its potential to induce mixed function oxidases in liver microsomes. These possibilities were examined by a pair-feeding protocol in which rats were fed diets with 35% of the caloric content as ethanol; control groups received a diet with an isocaloric amount of sucrose or were fed chow ad libitum. Alcohol caused a significant decrease in HTGL activity of liver microsomes, but there was no significant effect of alcohol upon the activities of LPL in adipose tissue and heart muscle. The relative rates of mixed function oxidases, assayed in control liver microsomes using ethoxy-,pentoxy- and benzyloxy-resorufin as substrates, were benzyloxy greater than ethoxy greater than pentoxy. This order was not affected by alcohol, but the oxidation of ethoxy- and pentoxyresorufin was reduced in liver microsomes from the ethanol-fed group. HTGL synthesis and secretion were also measured using primary rat hepatocyte cultures isolated from animals on the above dietary regimes and maintained for up to 3 days in basal medium alone or supplemented with 10 mmol/l ethanol. In basal media the order of activity of extracellular HTGL, released by the addition of heparin, was sucrose-fed greater than chow-fed greater than ethanol-fed. The rate of HTGL secretion from hepatocytes was stimulated in ethanol-containing medium, and was greater in hepatocytes from the sucrose-fed controls.(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":11933,"journal":{"name":"Enzyme","volume":"43 1","pages":"47-55"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000468704","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13510345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21

Acid lability of the mutated glucosylceramide-beta-glucosidase in a lymphoid cell line from type 2 Gaucher disease. 2型戈谢病淋巴样细胞系中突变的葡萄糖神经酰胺- β -葡萄糖苷酶的酸不稳定性。

Enzyme Pub Date : 1990-01-01 DOI: 10.1159/000468712

A Maret, R Salvayre, M Troly, L Douste-Blazy

引用次数: 1

Selective translation and degradation of heat-shock messenger RNAs in Drosophila. 果蝇热休克信使rna的选择性翻译和降解。

Enzyme Pub Date : 1990-01-01 DOI: 10.1159/000468754

S Lindquist, R Petersen

引用次数: 84

Enzymatic activities related to intermediary metabolism of glucose in circulating mononuclear cells from obese humans: relationship to enzyme activity in adipose tissue. 肥胖人群循环单核细胞中与葡萄糖中间代谢相关的酶活性:与脂肪组织中酶活性的关系。

Enzyme Pub Date : 1990-01-01 DOI: 10.1159/000468702

P Moghetti, E Bonora, M Cigolini, M Querena, V Cacciatori, M Muggeo

引用次数: 4

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