Enzyme Pub Date : 1990-01-01 DOI: 10.1159/000468762

J Curran, D Kolakofsky

引用次数: 35

Translation of poliovirus mRNA. 脊髓灰质炎病毒mRNA的翻译。

Enzyme Pub Date : 1990-01-01 DOI: 10.1159/000468765

N Sonenberg, K Meerovitch

引用次数: 28

Sialidase activity in rimantadine-resistant and -sensitive influenza A viruses. 金刚乙胺耐药和敏感甲型流感病毒唾液酸酶活性。

Enzyme Pub Date : 1990-01-01 DOI: 10.1159/000468733

A Garcia-Sastre, E Villar, C Hannoun, J A Cabezas

引用次数: 2

Differences in the renal handling of pancreatic and salivary amylase in the rat. 大鼠胰腺和唾液淀粉酶的肾脏处理差异。

Enzyme Pub Date : 1990-01-01 DOI: 10.1159/000468719

M Mályusz, P Wrigge, D Caliebe, J Das

引用次数: 2

Control of translation in adenovirus-infected cells. 腺病毒感染细胞中翻译的控制。

Enzyme Pub Date : 1990-01-01 DOI: 10.1159/000468763

M B Mathews

引用次数: 29

Translational frameshift suppression in mouse mammary tumor virus and other retroviruses. 小鼠乳腺肿瘤病毒和其他逆转录病毒的翻译移码抑制。

Enzyme Pub Date : 1990-01-01 DOI: 10.1159/000468768

J Majors

引用次数: 2

Coordinate post-transcriptional regulation of ferritin and transferrin receptor expression: the role of regulated RNA-protein interaction. 铁蛋白和转铁蛋白受体表达的转录后协调调控:受调控的rna -蛋白相互作用的作用。

Enzyme Pub Date : 1990-01-01 DOI: 10.1159/000468745

J B Harford, R D Klausner

{"title":"Coordinate post-transcriptional regulation of ferritin and transferrin receptor expression: the role of regulated RNA-protein interaction.","authors":"J B Harford, R D Klausner","doi":"10.1159/000468745","DOIUrl":"https://doi.org/10.1159/000468745","url":null,"abstract":"Excess iron results in an increase in the translation of ferritin mRNA and a decrease in the stability of transferrin receptor (TfR) mRNA. These coordinate regulatory events are mediated by similar sequence/structure motifs that exist within the 5' untranslated region (5'UTR) of the ferritin mRNA and the 3'UTR of the TfR mRNA. We have referred to these motifs as iron-responsive elements (IREs). The IREs from both transcripts interact with a cytoplasmic protein that we have called the IRE-binding protein (IRE-BP). The activity but not the amount of the IRE-BP is dependent on the cellular iron status. The biochemical basis for the altered activity of the IRE-BP appears to be the reversible oxidation-reduction of one or more cysteines in the IRE-BP. The IRE-BP is a 90- to 95-kD cytosolic protein that has been purified to homogeneity by RNA affinity chromatography, and the cDNA corresponding to the IRE-BP has been molecularly cloned. Collectively, our data support a model in which the interaction between the IRE-BP and the ferritin IRE results in attenuation of translation, and similar interaction with TfR mRNA can protect the transcript from rapid degradation mediated by a rapid turnover determinant within the 3'UTR.","PeriodicalId":11933,"journal":{"name":"Enzyme","volume":"44 1-4","pages":"28-41"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000468745","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13287639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 72

Deadenylylation: a mechanism controlling c-fos mRNA decay. 死基化:一种控制c-fos mRNA衰变的机制。

Enzyme Pub Date : 1990-01-01 DOI: 10.1159/000468756

M E Greenberg, A B Shyu, J G Belasco

引用次数: 27

Multiforms of mammalian adenylate kinase and its monoclonal antibody against AK1. 多种形式的哺乳动物腺苷酸激酶及其抗AK1单克隆抗体。

Enzyme Pub Date : 1990-01-01 DOI: 10.1159/000468708

Y Kurokawa, H Takenaka, M Sumida, K Oka, M Hamada, S A Kuby

{"title":"Multiforms of mammalian adenylate kinase and its monoclonal antibody against AK1.","authors":"Y Kurokawa, H Takenaka, M Sumida, K Oka, M Hamada, S A Kuby","doi":"10.1159/000468708","DOIUrl":"https://doi.org/10.1159/000468708","url":null,"abstract":"An attempt has been made to determine the intracellular distribution of the multiforms of the adenylate kinase (AK) isoenzymes in mammalian tissues, to shed some light on their physiological roles, especially in energy metabolism. The adenylate kinase zymograms obtained from isoelectric focusing yielded two typical isoform patterns: (1) with a pI greater than or equal to 9 and 8.6, specific for bovine skeletal muscle, heart, aorta and brain, and (2) with a pI = 7.9 and 7.1, specific for liver and kidney. Pattern (1) was attributed to the cytosolic isoenzyme (AK1) as demonstrated by immunostaining with anti-AK1. Pattern (2) was attributed to the mitochondrial isoenzyme (AK2). These results were largely confirmed by chromatofocusing experiments. The AK1 isoenzyme was partially purified from the cytosol fraction of bovine aortic smooth muscle and had an apparent Mr of 23.5 kilodaltons. Its kinetic features are discussed from a comparative standpoint. Finally, the human serum AK1 isoform was also detected by Western blotting with a monoclonal antibody directed against crystalline porcine muscle AK1. These results are to form the basis of further studies on the 'aberrant' adenylate kinase isoenzyme from the serum of Duchenne muscular dystrophics.","PeriodicalId":11933,"journal":{"name":"Enzyme","volume":"43 2","pages":"57-71"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000468708","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13413399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Purine synthesis de novo and salvage in hypoxanthine phosphoribosyltransferase-deficient mice. 次黄嘌呤磷酸核糖转移酶缺陷小鼠嘌呤的新生合成和回收。

Enzyme Pub Date : 1990-01-01 DOI: 10.1159/000468723

J Allsop, R W Watts

{"title":"Purine synthesis de novo and salvage in hypoxanthine phosphoribosyltransferase-deficient mice.","authors":"J Allsop, R W Watts","doi":"10.1159/000468723","DOIUrl":"https://doi.org/10.1159/000468723","url":null,"abstract":"Extreme degrees of hypoxanthine phosphoribosyltransferase (HPRT) deficiency in man are associated with gross sex-linked neurological dysfunction, gout and urinary stones (the Lesch-Nyhan or 'complete HPRT-deficiency' syndrome). The less severe degrees of enzyme deficiency (sex-linked recessive gout and/or urolithiasis or the 'partial HPRT-deficiency' syndrome) may be associated with minor neurological manifestations. Whole body purine synthesis de novo is accelerated in both these groups of patients. A strain of mice with an experimentally produced mutation at the HPRT locus showed some residual 'apparent HPRT activity' in brain, liver, testicular, splenic, kidney and ovarian tissues but not in erythrocyte haemolysates. The mutation removes exons 1 and 2 of the coding region of the gene together with the promotor and about 10 kb of upstream sequence from the gene. It is therefore possible that the observed 'apparent HPRT activity' in these mice is due to the operation of an alternative metabolic pathway. Purine synthesis de novo was markedly accelerated in their brain, testicular, splenic and kidney tissues. It was not accelerated in the liver tissue of male mice hemizygous for the mutation and the degree of acceleration in the female homozygotes only just reached statistical significance at the p = 0.02 level. This observation casts doubt on the importance of modulations in the rate of hepatic purine synthesis de novo as a mechanism for maintaining a steady supply of purines for translocation to other organs.","PeriodicalId":11933,"journal":{"name":"Enzyme","volume":"43 3","pages":"155-9"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000468723","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13250348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

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