Environmental and Experimental Botany最新文献

{"title":"High boron in irrigation water mitigates drought stress effects on 'Carrizo' citrange seedlings","authors":"M. Tasa ,&nbsp;M. González-Guzmán ,&nbsp;V. Arbona ,&nbsp;J.G. Pérez-Pérez","doi":"10.1016/j.envexpbot.2025.106228","DOIUrl":"10.1016/j.envexpbot.2025.106228","url":null,"abstract":"<div><div>Cultivating citrus in semi-arid areas exposes them to drought periods and sometimes irrigation with non-conventional water sources (e.g. reclaimed water and desalinated seawater) containing high boron (B) concentration. This study aims to determine how high B influences plant water relations and water transport in well-watered (WW) and drought-stressed (DS) ‘Carrizo’ citrange [<em>Citrus sinensis</em> (L.) Osb. × <em>Poncirus trifoliata</em> L.] seedlings during a stress period and after rewatering. Seedlings were well-watered with control B (0.11 mg·L⁻¹) or high B concentration (5 mg·L⁻¹) for 29 days. Then irrigation was withheld from half the plants for 7 days (drought-stressed, DS), followed by a 10-day re-irrigation period using control B. High B irrigation increased endogenous B accumulation, without reaching toxic levels as chlorophyll fluorescence parameters remained unaltered and plant water status was unaffected. However, partial stomatal closure mediated by a 1.8-fold increase in foliar ABA accumulation decreased transpiration and photosynthesis. Under drought stress, plants irrigated with high B concentration better maintained their leaf water status, with leaf water potential decreasing by only 0.9 MPa compared to 1.8 MPa decrease in Control B-DS plants relative to their respective well-watered plants. Thus, high B enhanced drought tolerance through ABA-mediated stomatal regulation, allowing more conservative water use during drought. Plant water status of high B plants recovered faster and more completely than control B plants, which experienced greater drought-induced damage.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"238 ","pages":"Article 106228"},"PeriodicalIF":4.7,"publicationDate":"2025-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144912570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seasonal changes rather than competition drive inorganic and organic nitrogen acquisition and internal allocation in temperate trees","authors":"Robert Reuter,&nbsp;Judy Simon","doi":"10.1016/j.envexpbot.2025.106227","DOIUrl":"10.1016/j.envexpbot.2025.106227","url":null,"abstract":"<div><div>For trees, soil nitrogen (N) acquisition and its internal allocation to different tissues are driven by species’ properties (e.g. growth rate, nutrient demand), the interactions among tree species, and their abiotic environment. Over the growing season, abiotic changes in, e.g. temperature and rainfall affect biogeochemical N cycling as well as N allocation and remobilisation processes in trees. We studied the influence of seasonality and developmental variation in woody seedlings, focussing on tree-tree interactions. We investigated the interaction effects among seven temperate European tree species on their inorganic and organic net N uptake capacity, internal allocation of N to metabolically active and storage tissues, and morphological traits at four leaf developmental stages over the growing season (i.e. before bud break, after leaf development, before / after leaf senescence). Seedlings of seven temperate European tree species were grown in mesocosms under natural forest conditions in intra- or interspecific competition. At the beginning of the growing season, organic N was favoured over inorganic N, whereas in autumn all N sources were taken up equally. Within species, N uptake and internal allocation generally varied over the growing season, except for N acquisition in <em>Carpinus</em>. Among tree species, the differences in N uptake and allocation were more related to species-specific morphological and physiological root traits, rather than functional properties. The outcome of the interactions was not generally affected within a tree community over the growing season but depended on the individual species. Thus, seasonal variation and species-specific properties should be considered for mixed species forests.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"238 ","pages":"Article 106227"},"PeriodicalIF":4.7,"publicationDate":"2025-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144912569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tissue expression pattern and regulatory function of ERD15 in response to UV-B signaling in Arabidopsis thaliana","authors":"Yuxin Xie ,&nbsp;Man Shu ,&nbsp;Can Yuan ,&nbsp;Yang Fang ,&nbsp;Wenjing Xu ,&nbsp;Lei Feng ,&nbsp;Hong Cai ,&nbsp;Tiantian Xiong","doi":"10.1016/j.envexpbot.2025.106226","DOIUrl":"10.1016/j.envexpbot.2025.106226","url":null,"abstract":"<div><div>Ultraviolet B band (UV-B) radiation, as an important component of the solar radiation spectrum and a key environmental factor, can regulate the expression of numerous genes and thus affects plant growth and development. Previous studies have shown that EARLY RESPONSIVE TO DEHYDRATION 15 (ERD15), acting downstream of BBX24, is known to mediate UV-B-induced hypocotyl growth inhibition, its precise regulatory function and molecular mechanisms within UV-B signaling remain unclear. In this study, we explored the tissue-specific expression patterns and regulatory roles of ERD15 in UV-B responses at the phenotypic, physiological, and transcriptional levels, revealing the regulatory network of ERD15 involvement in UV-B signaling. GUS staining revealed that UV-B radiation significantly suppressed ERD15 expression in hypocotyls and leaves, without causing obvious changes in roots. Phenotypic and physiological assessment further demonstrated that ERD15 inhibited the enhancement of leaf photosynthetic capacity under UV-B radiation. Transcriptome analysis indicated that ERD15 modulates UV-B responses through MAPK kinase, phytohormone, and antioxidant pathways, and interacts with multiple transcription factor families. Collectively, these findings elucidated the roles of ERD15 within the UV-B signaling regulatory network, and improved the current understanding of its molecular mechanisms.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"237 ","pages":"Article 106226"},"PeriodicalIF":4.7,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144895541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sulfonamides impede duckweed growth by inducing an excessive accumulation of salicylic acid","authors":"Aolin Ma,&nbsp;Siqi Liu,&nbsp;Zhixuan Du,&nbsp;Longfei Zhu,&nbsp;Guanping Feng","doi":"10.1016/j.envexpbot.2025.106224","DOIUrl":"10.1016/j.envexpbot.2025.106224","url":null,"abstract":"<div><div>Sulfonamides, owing to their therapeutic properties and cost-effectiveness, are widely employed in clinical settings and the animal husbandry industry. However, the misuse of these compounds has resulted in severe water pollution, thereby posing significant threats to the growth and development of aquatic plants such as duckweed. Our controlled experiments revealed that sulfonamide exposure disrupts the plasma membrane integrity in duckweed root tip cortex cells and inhibits the root growth. Utilizing ESI-HPLC-MS/MS, we identified that sulfonamide exposure elicits an immune response in duckweed, characterized by the generation of substantial amounts of salicylic acid (SA). The exogenous application of SA exacerbated the sulfonamide-induced growth inhibition, whereas the addition of the SA synthesis inhibitor 1-aminobenzotriazole (ABT) mitigated the stress induced by sulfonamides. A loss-of-function mutant of ICS1, a key enzyme in the SA biosynthesis pathway, exhibited complete insensitivity to sulfonamide exposure. Furthermore, transcription factors SARD1 and CBP60g, which regulate <em>ICS1</em> gene expression, were induced by sulfonamides, and their double mutant showed insensitivity to sulfonamide exposure. Reactive oxygen species (ROS) detection indicated abnormal accumulation of ROS in duckweed roots under sulfonamide exposure, which is likely to induce SA synthesis. These findings suggest that sulfonamide-mediated growth inhibition in plants is dependent on the ICS1-catalyzed SA biosynthesis pathway, thereby enhancing our understanding of the effects and mechanisms of sulfonamide exposure on aquatic plants.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"237 ","pages":"Article 106224"},"PeriodicalIF":4.7,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144893194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nitrogen fertilization modulates the endophytic bacteria of Aconitum carmichaelii Debx. and the quality of medicinal materials","authors":"Yichuan Liang ,&nbsp;Hongji Hou ,&nbsp;Guangfei Wei ,&nbsp;Yujia Fu ,&nbsp;Qian Wang ,&nbsp;Tianwei Su ,&nbsp;Lan Zou ,&nbsp;Jing Huang","doi":"10.1016/j.envexpbot.2025.106223","DOIUrl":"10.1016/j.envexpbot.2025.106223","url":null,"abstract":"<div><div><em>Aconitum carmichaelii</em> Debx. is a traditional medicinal plant with substantial economic value. Prolonged use of chemical fertilizers or high nitrogen (N) input may reduce the accumulation of pharmacodynamic compounds and adversely influence ecosystems. Although the impact of N fertilization on soil microorganisms has been extensively investigated, how N fertilization modulates the endophytic bacterial community and thus affects secondary metabolite accumulation in the host plant remains unclear. This study hypothesizes that moderate nitrogen fertilization enhances alkaloid accumulation by modulating endophytic bacterial communities, thereby altering plant metabolic pathways. The field experiment demonstrated that moderate N fertilization (N2: 350 kg N ha⁻¹) significantly increased the total alkaloid content in <em>A. carmichaelii</em> by 14.45 % (<em>P</em> &lt; 0.05) compared with that in the zero N control. High-throughput 16S rRNA gene sequencing indicated that moderate N fertilization significantly increased the diversity (Chao 1 and Shannon index, <em>P</em> &lt; 0.05) and altered the community composition (R² = 0.5081, <em>P</em> = 0.001) of endophytic bacteria. 64 endophytic bacterial taxa were identified differentially abundant taxa varying N treatments. Among them, 20 belonged to the N2 group, 16 of which demonstrated a strong positive correlation with alkaloid contents. Subsequent experiments confirmed that <em>Bacillus subtilis</em> JY-7–2L significantly enhanced alkaloid accumulation in <em>A. carmichaelii</em> (<em>P</em> &lt; 0.05) by producing alkaloids and inducing host metabolic pathways. Further analysis revealed that the metabolites from <em>B. subtilis</em> JY-7–2L remarkably up-regulated the genes related to signal transduction and alkaloid biosynthesis, thereby enhancing alkaloid accumulation in <em>A. carmichaelii</em>. This study demonstrated that rational fertilization significantly enhances plant–microbe interactions, particularly through the role of <em>B</em>. <em>subtilis</em> JY-7–2L in promoting alkaloid accumulation. These findings laid the foundation for optimizing the N fertilization strategy for <em>A. carmichaelii</em> cultivation and developing <em>B</em>. <em>subtilis</em> JY-7–2L strain into environmentally friendly biofertilizers to advance agricultural sustainability.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"238 ","pages":"Article 106223"},"PeriodicalIF":4.7,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145118189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In situ chemical imaging shows differential element mobilization in the rhizosphere of the Ni hyperaccumulator Odontarrhena chalcidica","authors":"Alice Tognacchini ,&nbsp;Markus Puschenreiter ,&nbsp;Thomas Prohaska ,&nbsp;Stefan Wagner","doi":"10.1016/j.envexpbot.2025.106221","DOIUrl":"10.1016/j.envexpbot.2025.106221","url":null,"abstract":"<div><div>Nickel (Ni) hyperaccumulating plants can accumulate Ni in their aboveground biomass at mass fractions exceeding 1000 μg g⁻¹ (dry weight). However, the processes controlling the acquisition of soil-borne Ni by hyperaccumulators remain poorly understood, particularly in relation to root-induced changes of rhizosphere chemistry. Using <em>in situ</em>, high-resolution chemical imaging via planar optodes and diffusive gradients in thin-films (DGT) combined with laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS), this study investigated localized changes in the spatial distribution of pH along with Ni, iron (Fe), zinc (Zn), and phosphorus (P) availability in the rhizosphere of the Ni hyperaccumulator <em>Odontarrhena chalcidica</em> grown on two ultramafic soils differing in pH (5.9 vs. 6.5) as well as total (552 vs. 1465 mg kg⁻¹) and extractable (42 vs. 158 mg kg⁻¹) Ni. Significant rhizosphere alkalinization of up to 1.5 pH units was observed at the immediate root surface in both soils, indicating root exudation of alkaline compounds. While Ni, Fe, and P fluxes were generally depleted around roots, increased Ni fluxes were observed only at root tips in the lower-Ni soil, highlighting a distinct biogeochemical niche for Ni mobilization. In contrast, increased Zn fluxes were observed consistently, irrespective of the soil or root type, revealing a previously unrecognized process for enhanced Zn availability in the rhizosphere. These findings suggest that <em>O. chalcidica</em> employs a highly selective rhizosphere modification strategy, combining pH shifts with element-specific mobilization mechanisms to control trace element availability and plant uptake.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"237 ","pages":"Article 106221"},"PeriodicalIF":4.7,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144892037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From the shoot to the rhizosphere: The short-term cascade impact of aerial microplastic","authors":"Oussama Bouaicha ,&nbsp;Fabio Trevisan ,&nbsp;Raphael Tiziani ,&nbsp;Martin Brenner ,&nbsp;Wolfram Weckwerth ,&nbsp;Elisabetta Onelli ,&nbsp;Alessandra Moscatelli ,&nbsp;Tanja Mimmo ,&nbsp;Luigimaria Borruso","doi":"10.1016/j.envexpbot.2025.106222","DOIUrl":"10.1016/j.envexpbot.2025.106222","url":null,"abstract":"<div><div>We hypothesized that foliar exposure to polyethylene microspheres (PEMS) in tomato plants (<em>Solanum lycopersicum</em> L.) triggers a cascade of physiological responses in leaves that ultimately reshape the root metabolome and exudate composition, thereby modulating the root-associated microbiome. Tomato plants were grown in soil and hydroponics. Leaves were sprayed with PEMS (10–100–1000 mg L⁻¹) or water (Control), avoiding direct contact with the growing media. After 31 days, biomass, SPAD, root metabolome, and rhizosphere microbial communities in soil-grown plants and exudome in hydroponic were assessed; root metabolome and exudates were analyzed via GC-MS, and rhizosphere communities via DNA metabarcoding. Additionally, shoots and roots were examined using transmission electron microscopy. Foliar PEMS exposure increased shoot and root biomass and SPAD index in the early days post-treatments, while reducing shoot water content, likely due to PEMS-induced ultrastructural cellular damage. In roots, PEMS significantly reduced the concentrations of several key metabolites, including serine, tryptophan, 5,6-dihydrouracil, lactic acid, tartaric acid, palmitic acid, and stearic acid. Root exudates also showed declines in isoleucine, malic, succinic, citric, aspartic, gluconic, and threonic acids. These changes significantly altered rhizobacterial alpha and beta diversity, notably enriching taxa linked to plant growth-promoting rhizobacteria (PGPR) functions. In contrast, fungal communities were unaffected, indicating lower responsiveness to short-term root exudate shifts. This underscores the short-term substantial impact of airborne microplastics on plant–rhizosphere system functioning. Overall, the aerial microplastics rapidly propagate effects from foliage to roots, altering belowground chemistry and selectively reshaping microbial communities, with potential consequences for nutrient cycling, plant health, and ecosystem resilience.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"237 ","pages":"Article 106222"},"PeriodicalIF":4.7,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144889137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spectral effects of green and blue light on lettuce morphology, growth, phytochemicals, and antioxidant capacity are temperature-dependent","authors":"Sang Jun Jeong ,&nbsp;Shuyang Zhen ,&nbsp;Qianwen Zhang ,&nbsp;Md Noor E. Azam Khan ,&nbsp;Genhua Niu","doi":"10.1016/j.envexpbot.2025.106214","DOIUrl":"10.1016/j.envexpbot.2025.106214","url":null,"abstract":"<div><div>Substituting green (G; 500–600 nm) for blue (B; 400–500 nm) light has been shown to enhance crop yield in indoor cultivation through increasing leaf expansion and photon capture. This effect is primarily mediated by cryptochrome photoreceptors. However, cryptochrome activity further depends on temperature. In our study, we investigated how B and G light and temperature interactively regulate plant growth, morphology, physiology, and phytochemical accumulation. Two lettuce (<em>Lactuca sativa</em>) cultivars, ‘Rex’ and ‘Rouxai’, were grown under three temperature conditions (20, 24, and 28 ℃) and five spectral treatments composed of B, G, and red (R; 600–700 nm) light (B₄₀G₀R₆₀, B₃₀G₁₀R₆₀, B₂₀G₂₀R₆₀, B₁₀G₃₀R₆₀, and B₀G₄₀R₆₀). The subscript number represents the percentage of each waveband in photosynthetic photon flux density (PPFD; 400–700 nm). PPFD was maintained at 200 μmol m⁻² s⁻¹ with 18-h photoperiod. Our results showed that light spectral quality and temperature interactively influenced lettuce growth and morphology. Within a moderate temperature range (20–24 ℃), substituting G for B light and warmer temperature synergistically enhanced leaf expansion and biomass production. However, at 28 ℃, higher G percentages (i.e., lower B percentages) caused excessive stem elongation and downward leaf rolling, limiting leaf development and overall growth. Moreover, substituting G for B light, combined with warmer temperature, synergistically decreased phytochemical accumulation and antioxidant capacity, likely due to a reduction in reactive oxygen species content and lower oxidative stress. These findings demonstrate that temperature influences cryptochrome-mediated morphological and physiological responses to light quality, which, in turn, affect plant growth and phytochemical accumulation.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"237 ","pages":"Article 106214"},"PeriodicalIF":4.7,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144889136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Natural genetic variation in nodal root growth angle and anatomy underlies drought tolerance in bread wheat","authors":"Md. Nurealam Siddiqui ,&nbsp;Abebaw M. Ambaw ,&nbsp;Asis Shrestha ,&nbsp;Kailash Pandey ,&nbsp;Tesfaye J. Teferi ,&nbsp;Melesech T. Gabi ,&nbsp;Diana Duarte-Delgado ,&nbsp;Jens Léon ,&nbsp;Agim Ballvora","doi":"10.1016/j.envexpbot.2025.106220","DOIUrl":"10.1016/j.envexpbot.2025.106220","url":null,"abstract":"<div><div>Root angle (RA) and anatomical attributes are pivotal for the acquisition and transport of water and nutrient during drought stress conditions. Here, we aimed to identify natural genetic variation and genomic loci associated with nodal RA and anatomical traits in response to drought stress. RA and anatomy traits were evaluated from nodal roots of the main shoot and tiller under well-watered and drought conditions in field. Marker-trait association were identified using a genome-wide association study (GWAS) coupled with linkage disequilibrium in a diverse set of 200 winter wheat genotypes. We observed a highly heritable change in RA and anatomy under drought in a genotype-dependent manner. Narrow RA and smaller cortex diameter of nodal root at the expense of increased stele diameter was the main adaptive response under drought stress. Increased stele diameter was caused by more and larger metaxylem vessel under drought than the control conditions. In addition, we observed a high phenotypic correlation in root anatomical traits of nodal roots between the main shoot and tillers, indicating possible overlapping genetic control. Next, we detected 24 significant marker-trait association for RA and anatomy traits resolved into 14 loci, indicating quantitative inheritance. Linkage disequilibrium and haplotype analysis revealed that the favorable phenotype at detected loci was represented by minor allele in the studied population. We identified 161 candidate genes and some of them were involved in root morphogenesis and differentiation. Of these, two promising candidate genes highly expressed in roots, namely, <em>TraesCS2A02G109700</em> and <em>TraesCS1B02G363700</em> are orthologs to auxin-responsive genes, <em>IBR3</em> and <em>IBR10</em>, respectively. They encode acyl-CoA dehydrogenase-like proteins that are required for root hair elongation and are potential targets for functional studies. The QTL detected in this study are reliable and can be used for marker-assisted selection of favorable root features for breeding drought-tolerant wheat cultivars.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"237 ","pages":"Article 106220"},"PeriodicalIF":4.7,"publicationDate":"2025-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144889138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Leveraging hyperspectral phenotyping for accurate, non-destructive prediction of metabolite profiles in poplar under drought stress","authors":"Mengjun Shu ,&nbsp;Antoine L. Harfouche ,&nbsp;Martin Trtílek ,&nbsp;Klára Panzarová ,&nbsp;Omar F. Alasia ,&nbsp;John H. Lagergren ,&nbsp;Audrey Labbé ,&nbsp;Nancy L. Engle ,&nbsp;Miranda M. Clark ,&nbsp;Jin-Gui Chen ,&nbsp;Gerald A. Tuskan ,&nbsp;Timothy J. Tschaplinski","doi":"10.1016/j.envexpbot.2025.106218","DOIUrl":"10.1016/j.envexpbot.2025.106218","url":null,"abstract":"<div><div>Accurately predicting drought tolerance in woody perennial bioenergy crops is critical for sustainable biomass production under fluctuating precipitation. Hyperspectral imaging (HSI) in the visible-near-infrared (VNIR) and shortwave-infrared (SWIR) ranges offers a promising approach for predicting plant biochemical traits, yet its application in metabolite profiling remains underexplored. We integrated VNIR+SWIR HSI with untargeted metabolomics to investigate drought-induced metabolic shifts in <em>Populus</em> leaves from eight <em>Populus</em> genotypes. Metabolite profiling identified 127 compounds, with 73 showing significant drought responses spanning amino acids (AA), carbohydrates (CHO), phenolic glycosides (PG), organic acids (OA), fatty acids and alcohols (FA), terpenes (T), phenolic metabolites (P), and unclassified metabolites. Spectral analysis revealed consistently higher reflectance across VNIR and SWIR wavelengths in drought-stressed plants, corresponding with increased accumulation of AA and reduced CHO and PG levels. Least absolute shrinkage and selection operator (LASSO) regression modeling identified robust spectral predictors of metabolite concentrations, associating VNIR wavelengths (500–700 nm) predominantly with AA and P, whereas SWIR wavelengths (1680–1700 nm) reliably predicted CHO, OA, and T. Several stable spectral-metabolite associations persisted across the two watering regimes (drought vs. well-watered), highlighting their potential as spectral biomarkers for non-destructive stress monitoring. Minimal genotype-specific variation suggests that observed spectral and metabolic responses were driven primarily by environmental factors, likely reflecting limited genetic diversity among the commercial <em>Populus</em> genotypes examined. This work establishes VNIR+SWIR hyperspectral imaging as a powerful, non-destructive phenotyping tool for precision monitoring and targeted improvement of drought resilience in bioenergy crops.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"237 ","pages":"Article 106218"},"PeriodicalIF":4.7,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144861220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}