Environmental and Experimental Botany最新文献

{"title":"Exogenously applied MT enhanced cold tolerance in tea plants by increasing fatty acid desaturation and polyamine metabolism","authors":"Kunlong Su ,&nbsp;Xiangzong Luo ,&nbsp;Yaning Lv ,&nbsp;Ziwen Zhou ,&nbsp;Xueqiao Song ,&nbsp;Ping Yang ,&nbsp;Yeyun Li ,&nbsp;Xianchen Zhang","doi":"10.1016/j.envexpbot.2025.106173","DOIUrl":"10.1016/j.envexpbot.2025.106173","url":null,"abstract":"<div><div>MT (melatonin), an important bioactive small molecule, plays crucial roles in plant responses to temperature or water stress. However, the intrinsic mechanisms underlying the modulatory effects of MT on physiological reactions are less clear. In this study, the mechanism by which exogenous MT modulates fatty acid and polyamine (PA) metabolism in tea plants under cold stress was studied. Application of 100 μM MT significantly increased the cold tolerance of tea plants. Additionally, PCPA (MT metabolic inhibitor) further aggravated cold-induced cell damage. Consistent with the change in phenotype, foliar application of MT significantly increased fatty acid unsaturation and PA levels compared with those under cold stress. In contrast, the MT metabolic inhibitor PCPA further impaired fatty acid desaturation and polyamine levels under the PCPA + cold treatment compared with those under the cold treatment. To further elucidate the role of MT in regulating fatty acid and PA metabolism, <em>CsCOMT3</em> (a key enzyme in MT synthesis)-silenced tea leaves were generated via virus-induced gene silencing (VIGS). Compared with the control leaves, <em>CsCOMT3</em>-inhibited tea leaves presented greater wilting and weaker chlorophyll fluorescence, with lower levels of fatty acid unsaturation and PAs. Our results clearly revealed that MT application may be a feasible strategy to relieve cold injury in tea plants by enhancing fatty acid and PA metabolism.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"236 ","pages":"Article 106173"},"PeriodicalIF":4.5,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144166368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling the impact of nano-microscale polyethylene and polypropylene plastics on Nicotiana tabacum: Physiological responses and molecular mechanisms","authors":"Muhammad Arshad ,&nbsp;Zelin Zhou ,&nbsp;Yichi Zhang ,&nbsp;Shaojie Lin ,&nbsp;Muhammad Shoaib ,&nbsp;Huijuan Zhang ,&nbsp;Weichang Gao ,&nbsp;Jun Wu ,&nbsp;Feng Hu ,&nbsp;Huixin Li","doi":"10.1016/j.envexpbot.2025.106169","DOIUrl":"10.1016/j.envexpbot.2025.106169","url":null,"abstract":"<div><div>Plastics, as emerging pollutants, are increasingly found in soil, yet their systemic impact on soil ecosystems and plants remains poorly understood. This study explores the impacts of Polypropylene (PP) and Polyethylene (PE) microplastics, of varying sizes (20 nm and 100 µm) and doses (100 and 1000 mg/kg), on tobacco plant growth. Over a 55-d exposure period, PP and PE MPs exhibited a dose-dependent effect on the growth of tobacco plants. Notably, both PE and PP exposures significantly suppressed plant height, as well as fresh and dry biomass, with PP demonstrating greater toxicity. However, an exception was observed in the PP treatment, with marginal yet notable increase in growth indicators was recorded at a 20 nm particle size under high-concentration exposure. Further investigations revealed that MPs exposure at varying concentrations negatively impacted photosynthetic activity and triggered oxidative stress in leaves, with higher-dose treatments leading to a more pronounced accumulation of reactive oxygen species (ROS). To elucidate the molecular response mechanisms of tobacco leaves under PP-MP stress, a co-omics analysis was conducted. The analysis identified key pathways involved in the plant’s response to PP-MP stress, including plant hormone signal transduction, the MAPK signaling, flavonoid and phenylpropanoid biosynthesis, and photosynthesis antenna proteins. A comprehensive assessment of genes and metabolites revealed significant alterations in the biosynthesis of several plant hormones and flavonoids, including auxin, cytokinin, abscisic acid (ABA), and jasmonic acid. These findings suggest that plastics may impair photosynthetic efficiency, alter hormonal responses, and cause redox imbalance, ultimately affecting plant growth and resilience.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"236 ","pages":"Article 106169"},"PeriodicalIF":4.5,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144116642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Variations in heterotrophic seedling growth parameters of 28 plant species and their relationships with seed traits","authors":"Elias Soltani ,&nbsp;Keyvan Maleki ,&nbsp;Nathalie Colbach ,&nbsp;Antoine Gardarin ,&nbsp;Jay Ram Lamichhane","doi":"10.1016/j.envexpbot.2025.106170","DOIUrl":"10.1016/j.envexpbot.2025.106170","url":null,"abstract":"<div><div>The timing of seedling emergence, influenced by heterotrophic growth, is a critical determinant for the competitive success of annual plants in arable cropping systems under temperate climates. While the heterotrophic growth phase is crucial for seedling establishment under field conditions, it has been much less explored compared to other growth phases. We address this gap by: i) analyzing the heterotrophic growth of primary seminal roots and shoots, focusing on their inter- and intra-specific diversity and growth synchrony, and ii) investigating the relationship between seed traits and heterotrophic growth parameters to explore how these traits influence growth dynamics. We synthesized data from 28 plant species, including 13 crops, 11 weeds, and four model plants, combining new datasets and published data. Inter-specific variation in heterotrophic root growth was assessed across all 28 species, while intra-specific variation in heterotrophic growth was examined in detail for seven species. We also explored the relationships between heterotrophic growth parameters (for both root and shoot) and seed traits, including seed mass and hydrothermal time model parameters — base water potential (Ψ_b) and base temperature (T_b) for germination. Our results showed that the maximum seedling length increased with an increase in seed mass or a decrease in either Ψ_b or T_b. The thermal time to mid-elongation, maximal shoot growth rate, and growth synchrony were significantly correlated to seed mass in weeds. This study enhances our ability to predict plant performance in agricultural systems, informs weed management strategies, and supports ecological forecasting in changing environments.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"236 ","pages":"Article 106170"},"PeriodicalIF":4.5,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144135025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Jasmonates signalling plays a key role in modulating berry terpenes accumulation under water deficit conditions in Vitis vinifera L. (cv. Sangiovese)","authors":"Giacomo Palai ,&nbsp;Joshua VanderWeide ,&nbsp;Joana Pico ,&nbsp;Simone Diego Castellarin ,&nbsp;Claudio D’Onofrio","doi":"10.1016/j.envexpbot.2025.106171","DOIUrl":"10.1016/j.envexpbot.2025.106171","url":null,"abstract":"<div><div>Terpenes are among the most important aroma compounds synthesized in grape berries and vine water status affects their accumulation. Although previous studies showed that exogenous hormones applications significantly affected terpenes accumulation, the signalling network involved in regulating their biosynthesis under drought conditions is still unclear. This experiment imposed several deficit irrigation treatments, to elucidate the role of the endogenous hormones signalling network in regulating berry terpenes biosynthesis under water deficit conditions. Berry monoterpenes increased when water deficit was applied during the green or the lag-phase of berry development. Berry abscisic acid and jasmonic acid concentrations were significantly increased by water deficit, regardless of when it was imposed, whereas salicylic acid showed significant differences between irrigation treatments only at veraison. Under water deficit conditions, correlation analyses suggested that jasmonates may be key hormones regulating the biosynthesis of berry monoterpenes, especially in the glycosylated form. Vines subjected to water deficit before veraison showed increased jasmonic acid and methyl jasmonate levels in berries, with the highest monoterpene concentrations measured in the same berries at harvest. These results suggested that endogenous jasmonates may play a key role in water deficit induced signalling that regulates berry monoterpenes biosynthesis.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"236 ","pages":"Article 106171"},"PeriodicalIF":4.5,"publicationDate":"2025-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144135026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Leaf chemical and structural properties govern foliar uptake of phosphorus from dust in chickpea","authors":"Elnatan Golan ,&nbsp;Avner Gross ,&nbsp;Nurit Agam ,&nbsp;Hagai Yasuor ,&nbsp;Ilana Shtein ,&nbsp;Ran Erel","doi":"10.1016/j.envexpbot.2025.106168","DOIUrl":"10.1016/j.envexpbot.2025.106168","url":null,"abstract":"<div><div>Certain plant species exhibit the capability to absorb phosphorus (P) directly from dust particles deposited on their leaves, particularly in P-limited environments. However, the underlying mechanisms that enable foliar uptake of P from insoluble sources such as dust minerals remain largely unexplored. This study investigates the chemical and structural properties of chickpea leaf surfaces that influence P absorption from dust deposition. We evaluated various chickpea varieties in a series of controlled experiments where P-rich dust was applied to their shoots. The relationship between P acquisition from dust and leaf surface pH, metabolite exudation and trichome density was examined. The tested varieties displayed variability in their leaf structural and chemical properties, which led to significantly different responses to foliar dust application. The most positive dust-responsive variety ('Mekomit') demonstrated a substantial 47–169 % increase in biomass in response to foliar dust application, whereas the most negatively responsive variety ('Cr205') exhibited a 28 % decrease in biomass following application. The positive response to dust was associated with lower leaf surface pH, higher trichome density, and enhanced metabolites exudation. Notably, the positively responsive variety showed increased exudation of sugars and organic acids, predominantly oxalic and malic acids from the leaf surfaces which facilitate P solubilization. These findings underscore the remarkable capacity of certain chickpea varieties to adapt their leaf characteristics in response to P deficiency, allowing them to absorb P from otherwise insoluble sources, such as airborne dust. This insight reveals a fundamental physiological mechanism that enhances our understanding of plant nutrient acquisition strategies under challenging environmental conditions.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"236 ","pages":"Article 106168"},"PeriodicalIF":4.5,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144146968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing citrus resilience to high temperature and intense light stress with Pseudomonas putida and Novosphingobium sp.","authors":"Fátima Terán ,&nbsp;Vicente Vives-Peris ,&nbsp;Aurelio Gómez-Cadenas ,&nbsp;Rosa M. Pérez-Clemente","doi":"10.1016/j.envexpbot.2025.106167","DOIUrl":"10.1016/j.envexpbot.2025.106167","url":null,"abstract":"<div><div>Adverse environmental conditions, aquifer contamination, and excessive fertilizer use have significantly impacted agricultural production, with these challenges worsening over the last few decades. Given the urgent need for more environmentally sustainable agricultural practices, recent studies have highlighted the potential of plant growth-promoting rhizobacteria (PGPR) as a biological alternative. PGPRs have been reported to function as biofertilizers and enhancers of plant tolerance to stress. Citrus, a globally important fruit crop, has been particularly affected by these environmental stressors. In this work, we aim to explore the effects of inoculating Cleopatra mandarin plants with PGPR strains <em>Pseudomonas putida</em> KT2440 and <em>Novosphingobium</em> sp. HR1a under combined conditions of heat stress and high light intensity (HSHL). Our findings demonstrate that inoculation with these strains provides significant protection against the detrimental effects of HSHL conditions. This protection is evident across multiple levels—phenotypically, physiologically, and molecularly—following the inoculation with <em>Pseudomonas putida</em> and <em>Novosphingobium</em> sp. Specifically, we observed under HSHL: (i) no leaf abscission in inoculated plants, (ii) improved photosynthesis efficiency under both non-stressed and stressed conditions, (iii) reduced malondialdehyde content, (iv) increased catalase activity, (v) upregulation of genes involved in stress tolerance (<em>WRKY40</em>, <em>WRKY76</em>, and <em>HSF30</em>), and (vi) increased sugar accumulation. Our study reveals that the use of <em>Pseudomonas putida</em> and <em>Novosphingobium</em> sp. offers a promising and environmentally sustainable approach for citriculture, particularly in mitigating the negative effects associated with climate change scenarios.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"235 ","pages":"Article 106167"},"PeriodicalIF":4.5,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143941158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alleviation of competitive constraints through long-term adaptation to high CO2 in mixed cultures of two diatom species","authors":"Yunyue Zhou ,&nbsp;Yuan Jia ,&nbsp;Peixuan Liu ,&nbsp;Baoyi Peng ,&nbsp;Jingyao Li ,&nbsp;Hao Zhang ,&nbsp;Leyao Xu ,&nbsp;Bin Huang ,&nbsp;Fangzhou Liu ,&nbsp;Jiamin Lin ,&nbsp;Fenghuang Wu ,&nbsp;Mengcheng Ye ,&nbsp;Jianrong Xia ,&nbsp;Peng Jin","doi":"10.1016/j.envexpbot.2025.106163","DOIUrl":"10.1016/j.envexpbot.2025.106163","url":null,"abstract":"<div><div>Diatoms play a pivotal role in marine ecosystems, contributing significantly to global primary production and carbon cycling. Understanding their responses to high CO₂ is critical for predicting oceanic changes under future climate scenarios. This study investigates the long-term adaptation of two diatom species, <em>Thalassiosira weissflogii</em> and <em>Phaeodactylum tricornutum</em>, to high CO₂ (1000 µatm) over 3.5–4 years and the consequences of their interactions in mixed cultures. Mono- and mixed-species cultures were maintained under both ambient (400 µatm) and high CO₂ conditions to assess various physiological performances. Our results revealed that most measured parameters (growth rate, photosynthesis and respiration rate, chlorophyll fluorescence parameters, and pigment concentration) were significantly reduced in mixed cultures compared to mono-cultures under both CO₂ conditions, underscoring the detrimental effects of interspecific competition. However, long-term adaptation to high CO₂ partially alleviated these reductions, particularly in photosynthesis, respiration, and chlorophyll-a content. These findings highlight the complex interplay between physiological adaptation and interspecific competition in shaping diatom responses to high CO₂. This study advances our understanding of the ecological and evolutionary implications of ocean acidification and underscores the importance of long-term experimental approaches for assessing the impacts of climate change on marine phytoplankton.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"235 ","pages":"Article 106163"},"PeriodicalIF":4.5,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143922047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single versus recurrent heat stress: A case study on the impact of a stress at flowering on the sorghum response to a subsequent post-flowering stress","authors":"Angelique Berger ,&nbsp;Belmys Cakpo ,&nbsp;Cyndi Suire ,&nbsp;Loic Pagan ,&nbsp;Sandrine Roques ,&nbsp;Raoul Vial ,&nbsp;Gregory Aguilar ,&nbsp;Nancy Terrier ,&nbsp;Christine Granier","doi":"10.1016/j.envexpbot.2025.106162","DOIUrl":"10.1016/j.envexpbot.2025.106162","url":null,"abstract":"<div><div>The increasing intensity and frequency of heat waves due to climate change pose a growing threat to global food security. Two experiments were conducted under controlled conditions using six temperature scenarios to compare the effects of single and recurrent heat stresses on maximum photosystem II quantum efficiency (<em>F</em>_v/<em>F</em>_m), above-ground plant biomass, grain yield and grain yield components in two sorghum genotypes. This study confirmed that the responses of grain number and thousand grain weight are highly dependent on stage development for both single and recurrent heat stresses. Recurrent heat stresses have synergistic effects, leading to around 20 % more yield losses than the cumulative responses to the corresponding single stresses. In contrast, <em>F</em>_v/<em>F</em>_m was less reduced by heat stress if the plants had been subjected to a previous stress. The large reduction in thousand grain weight under recurrent heat stresses may result in a strong remobilization of carbohydrates to sinks other than the main stem panicle. The increased tillering induced by single heat stresses and further amplified under recurrent heat stresses could partly explain this effect. The study highlights the complexity of plant responses to heat stress and the challenges of predicting outcomes under recurrent stress conditions.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"235 ","pages":"Article 106162"},"PeriodicalIF":4.5,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143941157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of soil water availability on apple tree physiology during heatwaves and on post-stress recovery","authors":"Nicola Giuliani ,&nbsp;Mario Wegher ,&nbsp;Dolores Asensio ,&nbsp;Damiano Zanotelli ,&nbsp;Carlo Andreotti ,&nbsp;Massimo Tagliavini","doi":"10.1016/j.envexpbot.2025.106161","DOIUrl":"10.1016/j.envexpbot.2025.106161","url":null,"abstract":"<div><div>In this study, the physiological response of potted apple trees to combined drought and heat stress was evaluated. After establishing different levels of soil water availability, the trees were exposed to a five-day simulated heatwave with daily maximum temperatures of 40°C. Stem water potential, leaf gas exchange, chlorophyll fluorescence, and tree transpiration were monitored before, during and after the combined application of heat and water stress, therefore providing insights into the extent and rapidity of the recovery. Drought caused stomatal closure that limited net photosynthesis and transpiration both at leaf and at tree level, leading to structural damage through leaf loss. On drought-stressed plants, chlorophyll fluorescence was significantly reduced by heat stress, suggesting additional leaf damage although net photosynthesis was not lower than under drought stress alone. On the other hand, well-watered trees showed low midday stem water potentials and high transpiration rates during the heatwave, while net photosynthesis was not affected. Water use efficiency of well-watered trees at 33°C was reduced to 60 % of that at 23°C. After the heatwave, transpiration rate in well-watered trees immediately declined to pre-stress levels, underscoring the strong atmospheric control on transpiration in apple trees. In drought-stressed trees, predawn stem water potential reached pre-stress values already on the first day of recovery. Stomatal conductance, net photosynthesis, and chlorophyll fluorescence, however, required a longer period to recover, indicating that drought stress induced transient hydraulic limitations. Nevertheless, all parameters fully recovered within five days after the end of the heatwave, showing that apple trees can withstand periods of combined heat and drought stress. The key role of water in modulating the response to heat stress highlights the need for improved irrigation management in apple orchards under climate change.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"235 ","pages":"Article 106161"},"PeriodicalIF":4.5,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143917659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genotypic variations in sensitivity of root K+ and Ca2+ transporters to H2O2 explains differential salt tolerance in wheat and barley","authors":"Haiyang Wang ,&nbsp;Ping Yun ,&nbsp;Lana Shabala ,&nbsp;Zhong-Hua Chen ,&nbsp;Meixue Zhou ,&nbsp;Sergey Shabala","doi":"10.1016/j.envexpbot.2025.106160","DOIUrl":"10.1016/j.envexpbot.2025.106160","url":null,"abstract":"<div><div>Wheat and barley are known as important staple food worldwide, but their growth and yield are severely affected by soil salinity, prompting a need for regaining their stress tolerance lost during domestication, to meet food security targets under current climate scenarios. The bottle neck in this process is plant phenotyping. In the past decades, approach to plant phenotyping for salinity stress tolerance was predominantly driven by the need for a high throughput screening and focused on the whole-plant level traits by advocating various non-destructive and/or analytical methods. This approach, though useful for assessing overall plant performance under salinity stress, fails to account for tissue- and cell-specific operation of contributing mechanisms and, as a result, lack the predictive power. In this work, we propose and validate a new approach for phenotyping cereal crops for salinity stress tolerance by measuring H₂O₂-induced K⁺ and Ca²⁺ flux responses from mature root epidermis. By screening 44 barley, 20 durum and 20 bread wheat accessions, we show that tolerant genotypes reduce sensitivity of cation (Na⁺, K⁺ and Ca²⁺) permeable ion channels to ROS and argue that such desensitization may allow plants to efficiently regulate its ionic homeostasis in a cell- and tissue-specific manner, without compromising stress-induced ROS signaling to downstream targets, for transcriptional regulation purposes. Being conducted on young (4-d old) seedlings, this cell-based phenotyping platform offer breeders a possibility to target new (previously unexplored) traits and may be instrumental for assisting breeders in engineering salinity stress tolerance in future breeding programs.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"235 ","pages":"Article 106160"},"PeriodicalIF":4.5,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143927601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}