Drug and Chemical Toxicology最新文献_第4页

Hepatic histopathological and morphometric changes in male mice exposed to rosuvastatin from pre-puberty to adulthood: a possible adaptive hepatic response. 雄性小鼠从青春期前到成年期暴露于洛伐他汀的肝组织病理学和形态计量学变化：一种可能的适应性肝反应。

IF 2.1 4区医学

Drug and Chemical Toxicology Pub Date : 2025-05-01 Epub Date: 2024-08-08 DOI: 10.1080/01480545.2024.2385609

Jennyfer Karen Knorst, Tainara Fernandes de Mello, Amanda Rebonatto Oltramari, Alice Santos da Silva, Gabriel Adan Araújo Leite

{"title":"Hepatic histopathological and morphometric changes in male mice exposed to rosuvastatin from pre-puberty to adulthood: a possible adaptive hepatic response.","authors":"Jennyfer Karen Knorst, Tainara Fernandes de Mello, Amanda Rebonatto Oltramari, Alice Santos da Silva, Gabriel Adan Araújo Leite","doi":"10.1080/01480545.2024.2385609","DOIUrl":"10.1080/01480545.2024.2385609","url":null,"abstract":"Lifestyle changes, such as poor eating habits and a reduction in physical exercise, have impaired human lipid profiles. Statins are widely used to treat dyslipidemias, of which rosuvastatin shows greater improvement in the lipid profile and may be used since childhood. This study aimed to assess the hepatic effects when male mice were given 0.9% saline solution or doses of rosuvastatin of 1.5 or 5.5 mg/kg/day from postnatal day (PND) 23 until PND 80. Body mass gain and water and food consumption were monitored during the treatment. Mice were euthanized on PND 80 when blood was collected for serum obtainment, and several organs were collected and weighed. Serum was used for evaluating lipid profiles and markers of hepatic injuries. The liver was assessed for histopathological, morphometric, and stereological changes. There was a temporary reduction in body mass gain and water and food consumption in the rosuvastatin-exposed groups. Both rosuvastatin-treated groups exhibited reduced total cholesterol levels and showed signs of hepatic tissue adaptation in response to prolonged exposure, such as sinusoidal dilation, inflammatory infiltrates, and cell death of hepatocytes. These results are considered side effects of the treatment and may indicate a hepatic adaptation to the chronic exposure.","PeriodicalId":11333,"journal":{"name":"Drug and Chemical Toxicology","volume":" ","pages":"557-570"},"PeriodicalIF":2.1,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141901228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcription of biological aging markers (ANRIL, P16^INK4a, TBX2, and TERRA) and their correlations with severity of sulfur mustard exposure in veterans. 退伍军人生物衰老标志物（ANRIL、P16INK4a、TBX2 和 TERRA）的转录及其与硫芥子气暴露严重程度的相关性。

IF 2.1 4区医学

Drug and Chemical Toxicology Pub Date : 2025-05-01 Epub Date: 2024-09-03 DOI: 10.1080/01480545.2024.2395571

Leila Nasiri, Mohammad-Reza Vaez-Mahdavi, Hossein Hassanpour, Tooba Ghazanfari, Sussan Kaboudanian Ardestani, Nayere Askari, Sara Ghaffarpour, Mohammad Saber Zamani

{"title":"Transcription of biological aging markers (ANRIL, P16INK4a, TBX2, and TERRA) and their correlations with severity of sulfur mustard exposure in veterans.","authors":"Leila Nasiri, Mohammad-Reza Vaez-Mahdavi, Hossein Hassanpour, Tooba Ghazanfari, Sussan Kaboudanian Ardestani, Nayere Askari, Sara Ghaffarpour, Mohammad Saber Zamani","doi":"10.1080/01480545.2024.2395571","DOIUrl":"10.1080/01480545.2024.2395571","url":null,"abstract":"Sulfur mustard (SM) exposure has delayed harmful effects, including premature biological aging. This study aimed to evaluate the expression of aging markers (i.e., ANRIL, P16INK4a, TBX2, and TERRA) and assess their correlation with the severity of SM exposure in the long term. The study was conducted on two volunteer groups. 1) SM-exposed group, exposed to SM once in 1987 during the war; divided into three subgroups based on the injury severity, asymptomatic (without any clinical signs), mild, and severe; 2) Non-exposed group. In the SM-exposed group, ANRIL transcript was decreased, especially in subgroups of mild and severe. TBX2 transcript was also decreased in the total SM-exposed group. This decrease was more significant in the mild and severe subgroups than in asymptomatic ones. P16INK4a transcript was increased in the SM-exposed group, especially in the asymptomatic subgroup. The increase in TERRA transcript was also significant in all subgroups. There was a positive correlation between the TERRA transcript and the severity of injury, while this correlation was negative for the ANRIL. It is concluded that the delayed toxicity of SM may be associated with dysregulation of aging markers leading to premature cellular aging. These markers' alterations differed according to the severity of SM injury.","PeriodicalId":11333,"journal":{"name":"Drug and Chemical Toxicology","volume":" ","pages":"668-676"},"PeriodicalIF":2.1,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142125146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reproductive and developmental toxicity of α-terpineol in Wistar rats. α-松油醇对 Wistar 大鼠生殖和发育的毒性。

IF 2.1 4区医学

Drug and Chemical Toxicology Pub Date : 2025-05-01 Epub Date: 2024-10-16 DOI: 10.1080/01480545.2024.2412022

Sneha Suma Hegde, Vijaykumar B Malashetty

{"title":"Reproductive and developmental toxicity of α-terpineol in Wistar rats.","authors":"Sneha Suma Hegde, Vijaykumar B Malashetty","doi":"10.1080/01480545.2024.2412022","DOIUrl":"10.1080/01480545.2024.2412022","url":null,"abstract":"Alpha-terpineol (α-T) is a type of monoterpenoid alcohol commonly present in essential oils, it contributes to a pleasant floral fragrance similar to that of lilacs. This research aimed to evaluate the impact of α-terpineol on the reproductive functions of both male and female rats, including gonadal activity, mating behavior, conception, conceptus development and parturition. Six male and female Wistar rats per group received α-terpineol through gavage at doses of 0, 75, 150 and 300 mg/kg/day. The study revealed changes in body weight gain inhibition, food consumption, azoospermia, decreased testosterone levels (0.7 ± 1.61 ng/mL, 0.7 ± 2.30 ng/mL) as well as histopathological variations in testis and epididymis among males exposed to doses of 150 and 300 mg/kg/day. Moreover, this exposure led to significantly decreased serum T4 levels in both adult males (21.85 ± 12.68 ng/mL, 16.20 ± 9.15 ng/mL) and dams (11.24 ± 12.37 ng/mL, 9.48 ± 11.74 ng/mL) at the dose range of 150 and 300 mg/kg/day without affecting TSH concentrations. In summary, the present study showed that α-terpineol induced reproductive toxicities in male rats. Therefore, a detailed toxicological assessment is highly recommended.","PeriodicalId":11333,"journal":{"name":"Drug and Chemical Toxicology","volume":" ","pages":"488-505"},"PeriodicalIF":2.1,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142460506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro and in vivo genotoxicity assessments of Ficus erecta Thunb. leaves extract support its functional and pharmaceutical potential. 榕树体内外遗传毒性评价。叶提取物支持其功能和药物潜力。

IF 2.1 4区医学

Drug and Chemical Toxicology Pub Date : 2025-04-29 DOI: 10.1080/01480545.2025.2495363

Mee-Young Lee, Yu Jin Kim, Woo-Young Jeon, Eunjin Sohn, Sae-Rom Yoo, Soo-Jin Jeong

{"title":"In vitro and in vivo genotoxicity assessments of Ficus erecta Thunb. leaves extract support its functional and pharmaceutical potential.","authors":"Mee-Young Lee, Yu Jin Kim, Woo-Young Jeon, Eunjin Sohn, Sae-Rom Yoo, Soo-Jin Jeong","doi":"10.1080/01480545.2025.2495363","DOIUrl":"https://doi.org/10.1080/01480545.2025.2495363","url":null,"abstract":"Ficus erecta Thunb. has been used to treat various inflammatory diseases owing to its anti-inflammatory and antioxidative properties. However, scientific evidence on the safety of F. erecta extract is limited. We evaluated the genotoxicity of ethanol extract of F. erecta (EEFE) using multiple tests. The bacterial reverse mutation (Ames) test showed that EEFE treatment of five bacterial strains (Salmonella typhimurium TA98, TA100, TA1535, TA1537, and Escherichia coli WP2uvrA) did not affect the number of revertant colonies in the absence or presence of S9 mix, a metabolic activator, up to a concentration of 312.5 µg/plate. The number of chromosomal aberrations associated with EEFE was similar to that in the negative controls, with no significant increase up to a concentration of 31.3 µg/mL EEFE. The chromosomal aberration assay showed that EEFE was not associated with any abnormalities in combination with or without S9 mix. The in vivo micronucleus test revealed that EEFE did not influence the frequency of bone marrow micronuclei up to 2000 mg/kg body weight of male ICR mice. Moreover, no changes in mouse body weight or mortality rates were observed. Our study demonstrates the safety of EEFE in genotoxicity tests, providing proof-of-concept for its use as a medicine and functional food.","PeriodicalId":11333,"journal":{"name":"Drug and Chemical Toxicology","volume":" ","pages":"1-10"},"PeriodicalIF":2.1,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143959125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Computational investigation of m-acetamide and 3MPAEA: Characterization, toxicity, and molecular docking and dynamic analyses. 间乙酰胺和3MPAEA的计算研究：表征、毒性、分子对接和动力学分析。

IF 2.1 4区医学

Drug and Chemical Toxicology Pub Date : 2025-04-28 DOI: 10.1080/01480545.2025.2496358

Nevin Çankaya, Mehmet Hanifi Kebiroğlu, Serap Yalcin Azarkan

{"title":"Computational investigation of m-acetamide and 3MPAEA: Characterization, toxicity, and molecular docking and dynamic analyses.","authors":"Nevin Çankaya, Mehmet Hanifi Kebiroğlu, Serap Yalcin Azarkan","doi":"10.1080/01480545.2025.2496358","DOIUrl":"https://doi.org/10.1080/01480545.2025.2496358","url":null,"abstract":"In this study, 2-(3-methoxyphenylamino)-2-oxoethyl acrylate (3MPAEA) molecule was synthesized in two steps. In the first step, 2-chloro-N-(3-methoxyphenyl)acetamide (m-acetamide) was obtained. Density functional theory (DFT) calculations were performed to obtain information about the electronic and structural properties of the synthesized molecules. The Raman Spectrum and UV-Visible analysis were calculated using the Gaussian package program. Additionally, Natural Bond Orbital (NBO) Analysis, Electron Localization Function (ELF), Electrostatic Potential Map (ESP), Average Local Ionization Energy (ALIE), and the toxicological properties of the molecules were examined. Simultaneously, molecular docking and dynamic analyses were conducted to investigate the interaction of m-acetamide and 3MPAEA with proteins involved in nuclear receptor signaling pathways, stress response pathways, molecular initiating events, and metabolism, as identified in the protox analysis. The findings aligned with the protox analysis results. The results obtained provide new insights into the electronic and toxicological properties of these molecules.","PeriodicalId":11333,"journal":{"name":"Drug and Chemical Toxicology","volume":" ","pages":"1-16"},"PeriodicalIF":2.1,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143971604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of bioinformatics techniques to discovery of mechanisms by which plasticizers promote acute myelogenous leukemia. 生物信息学技术在发现增塑剂促进急性骨髓性白血病机制中的应用。

IF 2.1 4区医学

Drug and Chemical Toxicology Pub Date : 2025-04-26 DOI: 10.1080/01480545.2025.2496324

Yi Zhan, Jiandong Shi

{"title":"Application of bioinformatics techniques to discovery of mechanisms by which plasticizers promote acute myelogenous leukemia.","authors":"Yi Zhan, Jiandong Shi","doi":"10.1080/01480545.2025.2496324","DOIUrl":"https://doi.org/10.1080/01480545.2025.2496324","url":null,"abstract":"This study aims to elucidate the potential roles of commonly used plasticizers, including Diethyl Phthalate (DEP), Dimethyl Phthalate (DMP), and Dioctyl Phthalate (DOP), in the pathogenesis of Acute Myeloid Leukemia (AML). The focus is to highlight the complex interactions between these environmental chemicals and key molecular pathways involved in tumorigenesis. We employed network toxicology and molecular docking techniques to analyze the interactions between plasticizers and key proteins associated with AML. Utilizing databases such as The Cancer Genome Atlas (TCGA), we divided selected key genes from AML bone marrow samples into two groups based on gene expression and compared their survival analyses. Enrichment analysis was conducted to identify the biological pathways associated with these genes. The enrichment analysis underscored the association between the plasticizer-targeted genes and essential pathways in AML development, indicating a broad impact of plasticizers on various cancers, including hematologic malignancies. Subsequent expression analysis using TCGA data for AML demonstrated that these genes have significant statistical relevance to the survival in AML, confirming their critical roles in tumor biology. This study provides evidence that exposure to plasticizers could influence the pathogenesis of AML through interactions with key proteins and signaling pathways. By utilizing network pharmacology and protein interaction analysis, our findings emphasize the potential risks associated with plasticizers. These results highlight the necessity for further epidemiological and clinical research to fully understand the impact of plasticizer exposure on AML risk, thereby informing future preventive and therapeutic strategies.","PeriodicalId":11333,"journal":{"name":"Drug and Chemical Toxicology","volume":" ","pages":"1-10"},"PeriodicalIF":2.1,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143973444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ginkgo biloba leaf extract alleviates paraquat-induced RLE-6TN cell damage via the miR-155-5p/SIRT1/TLR4 axis. 银杏叶提取物通过miR-155-5p/SIRT1/TLR4轴缓解百草枯诱导的RLE-6TN细胞损伤。

IF 2.1 4区医学

Drug and Chemical Toxicology Pub Date : 2025-04-14 DOI: 10.1080/01480545.2025.2489032

Genlin Liu, Ting Sun, Hong Zhou, Anmin Liu, Le Li

{"title":"Ginkgo biloba leaf extract alleviates paraquat-induced RLE-6TN cell damage via the miR-155-5p/SIRT1/TLR4 axis.","authors":"Genlin Liu, Ting Sun, Hong Zhou, Anmin Liu, Le Li","doi":"10.1080/01480545.2025.2489032","DOIUrl":"https://doi.org/10.1080/01480545.2025.2489032","url":null,"abstract":"Ginkgo biloba extract (GBE) has demonstrated therapeutic potential in paraquat (PQ)-induced injury; however, its molecular mechanism remains unclear. The cell viability, lactate dehydrogenase (LDH) release, and apoptosis of RLE-6TN cells were assessed using CCK-8, LDH assay, and flow cytometry. Oxidative stress was evaluated using the DCFH-DA probe, while inflammatory cytokine levels (TNF-α, IL-1β, and IL-6) were measured via ELISA. The expression of miR-155-5p was analyzed using qRT-PCR. Bioinformatics analysis, dual-luciferase reporter assay, and RNA immunoprecipitation (RIP) assay were employed to investigate the interaction between SIRT1 and miR-155-5p. Protein expression levels of SIRT1, TLR4, phosphorylated P65 (p-P65), and total P65 were determined by western blotting. The results showed that miR-155-5p expression was upregulated in PQ-induced cell injury. Knockdown of miR-155-5p improved cell viability and reduced apoptosis, oxidative stress, and inflammatory damage in PQ-treated cells. In RLE-6TN cells, miR-155-5p directly targeted the SIRT1/TLR4 axis. GBE mitigated PQ-induced cell injury by modulating the miR-155-5p/SIRT1/TLR4 axis, thereby enhancing cell viability and reducing apoptosis, oxidative stress, and inflammation. This study indicates that GEB may protect against PQ-induced RLE-cell injury by regulating the miR-155-5p/SIRT1/TLR4 signaling pathway and that miR-155-5p could be a promising biomarker for PQ intoxication, providing novel insights into potential therapeutic strategies for PQ poisoning.","PeriodicalId":11333,"journal":{"name":"Drug and Chemical Toxicology","volume":" ","pages":"1-10"},"PeriodicalIF":2.1,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143986982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of cytotoxic properties of tetrahydroisoquinoline oximes in breast, prostate and glioblastoma cancer cells. 四氢异喹啉肟对乳腺、前列腺和胶质母细胞瘤癌细胞的细胞毒性评估。

IF 2.1 4区医学

Drug and Chemical Toxicology Pub Date : 2025-04-14 DOI: 10.1080/01480545.2025.2491534

Antonio Zandona, Matea Jurić, Ludovic Jean, Pierre-Yves Renard, Maja Katalinić

{"title":"Assessment of cytotoxic properties of tetrahydroisoquinoline oximes in breast, prostate and glioblastoma cancer cells.","authors":"Antonio Zandona, Matea Jurić, Ludovic Jean, Pierre-Yves Renard, Maja Katalinić","doi":"10.1080/01480545.2025.2491534","DOIUrl":"https://doi.org/10.1080/01480545.2025.2491534","url":null,"abstract":"Tetrahydroisoquinoline (THIQ) oximes have been investigated as antidotes for poisoning by toxic organophosphorus compounds. Recent studies have shown that some THIQ oximes induce cytotoxic effects and trigger apoptosis in various cell types. Since this pathway activation is desirable for anticancer drugs, we further explored the effects of three selected THIQ oximes on well-known cancer cell models: breast (MDA-MB-231 and MCF-7), prostate (PC-3) cancer and malignant glioblastoma (U251). The tested THIQ oximes were cytotoxic to breast cancer cells and, to a lesser extent, to glioblastoma cells, but not to PC-3 cells at concentrations up to 200 µM within a 24-h exposure period. The MCF-7 cells exhibited the highest sensitivity, with all three oximes affecting it in a time-dependent manner (IC50 from 7-74 µM). While the membrane integrity of affected cells was maintained after treatment with the tested THIQ oximes, they disrupted mitochondrial membrane potential and activated caspase 9 indicating triggering of the mitochondria-mediated apoptosis. Overall, these findings suggest that the THIQ oxime scaffold could be a foundation for developing targeted therapies, especially for breast cancer, and other derivatives may be worthier of exploration.","PeriodicalId":11333,"journal":{"name":"Drug and Chemical Toxicology","volume":" ","pages":"1-8"},"PeriodicalIF":2.1,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143991689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective effect of Leuco-methylene blue against acetaminophen-induced liver injury: an experimental study. 亚甲基蓝对对乙酰氨基酚所致肝损伤的保护作用的实验研究。

IF 2.1 4区医学

Drug and Chemical Toxicology Pub Date : 2025-04-10 DOI: 10.1080/01480545.2025.2485347

Majid Efati, Amirhossein Sahebkar, Shima Tavallaei, Soodeh Alidadi, Hossein Hosseini, Daryoush Hamidi-Alamdari

{"title":"Protective effect of Leuco-methylene blue against acetaminophen-induced liver injury: an experimental study.","authors":"Majid Efati, Amirhossein Sahebkar, Shima Tavallaei, Soodeh Alidadi, Hossein Hosseini, Daryoush Hamidi-Alamdari","doi":"10.1080/01480545.2025.2485347","DOIUrl":"https://doi.org/10.1080/01480545.2025.2485347","url":null,"abstract":"Acetaminophen is a commonly used drug for mild to moderate pain relief; however, acetaminophen toxicity due to the formation of toxic metabolites is a major cause of drug-induced liver injury. Methylene blue is an FDA-approved drug for the treatment of methemoglobinemia and has potential applications in the treatment of carbon monoxide and cyanide poisoning. Leuco-methylene blue, a colorless form of methylene blue, is more effective in entering cells and counteracting oxidative stress, making it a valuable option in regulating mitochondrial function and ATP production. In this study, we aimed to evaluate the effect of LMB on liver damage caused by acetaminophen toxicity. Thirty-six rats were divided into six groups: control, APAP, NAC, LMB, MB, and NAC+LMB. All groups except the control received acetaminophen (1500 mg/kg), followed by treatments with NAC (100 mg/kg), LMB (5 mg/kg), MB (5 mg/kg), and NAC+LMB after 3 hours. The rats were sacrificed 24 hours post-acetaminophen administration. LMB significantly reduced serum levels of liver enzymes (ALT, AST, and ALP) and increased the expression of genes involved in mitochondrial biogenesis and antioxidant defense (PGC-1, Nrf2, and Tfam). Additionally, LMB significantly increased total antioxidant capacity and glutathione reductase levels, decreased the prooxidant-antioxidant balance (PAB), and reduced the expression of inflammatory cytokines (IL-6 and TNF-α) in the liver tissue. LMB effectively reduced the severity of acetaminophen-induced liver damage through antioxidant and anti-inflammatory effects. LMB can effectively ameliorate APAP-induced toxicity in rats, with comparable efficacy to N-acetylcysteine with respect to most complications of acetaminophen-induced toxicity in rats.","PeriodicalId":11333,"journal":{"name":"Drug and Chemical Toxicology","volume":" ","pages":"1-13"},"PeriodicalIF":2.1,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143968921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phytochemical profiling of Sansevieria trifasciata and its efficacy against Dynapar-induced toxicity to Danio rerio. 三海参的植物化学特征及其对达纳帕的抗毒作用。

IF 2.1 4区医学

Drug and Chemical Toxicology Pub Date : 2025-04-10 DOI: 10.1080/01480545.2025.2487875

Vandana Dohre, Surabhi Yadav, Kaneez Zahra

{"title":"Phytochemical profiling of Sansevieria trifasciata and its efficacy against Dynapar-induced toxicity to Danio rerio.","authors":"Vandana Dohre, Surabhi Yadav, Kaneez Zahra","doi":"10.1080/01480545.2025.2487875","DOIUrl":"https://doi.org/10.1080/01480545.2025.2487875","url":null,"abstract":"Sansevieria trifasciata (S. trifasciata) has been widely recognized in traditional medicinal systems for its therapeutic potential from the Bundelkhand region of India. The current study investigates the potential efficacy of S. trifasciata against Dynapar (diclofenac)-induced toxicity. We conducted phytochemicals screening and gas chromatography-mass spectrometry (GC-MS) analysis of S. trifasciata leaf aqueous extract (STLAE), revealing diverse bioactive compounds, including saponins, phenols, tannins, glycosides, carbohydrates, and terpenoids. GC-MS analysis further identified 10 specific compounds, notably nitrogen-containing heterocycles, esters, amides, and methoxyacetic acid derivatives, providing molecular insights into the plant's traditional medicinal applications. Using zebrafish (Danio rerio) as an ethically sound vertebrate model, we established Dynapar's 96-hour median lethal concentration (LC50) at 1.5 mL/L (112.5 mg/L). Co-administration experiments demonstrated that STLAE at 2.2 mL/L (220 mg/L) completely prevented Dynapar-induced mortality, suggesting a defined therapeutic window for protective effects. Interestingly, STLAE showed a dose-dependent response curve, with lower and higher concentrations exhibiting reduced protection, highlighting the importance of precise dosing in natural product applications. Our findings provide the first experimental evidence supporting S. trifasciata's traditional use in treating inflammatory conditions and suggest its potential as a natural intervention against NSAID-induced toxicity. The identified nitrogen-containing compounds and other phytoconstituents likely contribute to the observed protective effects by modulating oxidative stress pathways implicated in Dynapar toxicity. This work establishes groundwork for further investigation into S. trifasciata as a safer alternative for managing chronic inflammatory conditions.","PeriodicalId":11333,"journal":{"name":"Drug and Chemical Toxicology","volume":" ","pages":"1-10"},"PeriodicalIF":2.1,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143996211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0