Drug and Chemical Toxicology最新文献

Synergistic toxicity of glyphosate- and 2,4-Dichlorophenoxyacetic acid-based formulations on Caenorhabditis elegans.

IF 2.1 4区医学

Drug and Chemical Toxicology Pub Date : 2025-04-07 DOI: 10.1080/01480545.2025.2486542

Pamella Patricia Fuzzer Figueiredo, Laura Cé da Silva, Júlia Menezes, Joana Oliveira Machado, Roberta Rodrigues Zorzo, Mariana Arend Schmitt, Gabriela Endres da Rocha Pompeo, Solange Cristina Garcia, Simone Gasparin Verza, Gunther Gehlen, Natália Brucker, Mariele Feiffer Charão

{"title":"Synergistic toxicity of glyphosate- and 2,4-Dichlorophenoxyacetic acid-based formulations on Caenorhabditis elegans.","authors":"Pamella Patricia Fuzzer Figueiredo, Laura Cé da Silva, Júlia Menezes, Joana Oliveira Machado, Roberta Rodrigues Zorzo, Mariana Arend Schmitt, Gabriela Endres da Rocha Pompeo, Solange Cristina Garcia, Simone Gasparin Verza, Gunther Gehlen, Natália Brucker, Mariele Feiffer Charão","doi":"10.1080/01480545.2025.2486542","DOIUrl":"https://doi.org/10.1080/01480545.2025.2486542","url":null,"abstract":"The use and marketing of herbicides are increasing worldwide. Glyphosate and 2,4 dichlorophenoxyacetic acid (2,4D) are the most prominent herbicides in this progression, used alone and in combination. There are few reports on the effects of these herbicides in combination. The Caenorhabditis elegans (C. elegans) experimental model has a complete system and is easy to manipulate and maintain. This study evaluated the toxicity of glyphosate and 2,4D alone and in combination with the parameters of survival, development, and reproduction in C. elegans at 4 different concentrations of the herbicides (0.115-0.925 mg/mL for glyphosate and from 1.046 to 8.375 mg/mL for 2,4D). The nature herbicides' interaction was evaluated using SynergyFinder software with the Highest Single Agent (HSA) probabilistic model. The survival results indicated an LC50 (Lethal Concentration 50) of 0.66 mg/mL for glyphosate alone and 0.164 mg/mL when combined with 2,4D. The LC50 for 2,4D alone was 9.41 mg/mL and 3.011 mg/mL when combined with glyphosate. There was a significant reduction in nematode development and reproduction as well as greater herbicide toxicity when combined (p < 0.05). The HSA indicated synergistic effects at different concentrations of the mixture, demonstrating greater toxicity of these herbicides in combination. This is the first time that the synergistic effect of glyphosate and 2,4D on C. elegans has been reported in the literature. This highlights the importance of further studies to understand the impact of using these herbicides alone and in combination, as they are widely used and can affect populations and ecosystems.","PeriodicalId":11333,"journal":{"name":"Drug and Chemical Toxicology","volume":" ","pages":"1-10"},"PeriodicalIF":2.1,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143794757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histological and flow cytometric evaluation of astaxanthin's effects against cyclophosphamide induced heart injury in rats.

IF 2.1 4区医学

Drug and Chemical Toxicology Pub Date : 2025-04-07 DOI: 10.1080/01480545.2025.2487865

Tugba Zengin, Yavuz Tekelioglu, Oguzhan Keskin, Göksen Derya Reis Kose, Neziha Senem Ari, Tugba Arici, Dilan Cetinavci

{"title":"Histological and flow cytometric evaluation of astaxanthin's effects against cyclophosphamide induced heart injury in rats.","authors":"Tugba Zengin, Yavuz Tekelioglu, Oguzhan Keskin, Göksen Derya Reis Kose, Neziha Senem Ari, Tugba Arici, Dilan Cetinavci","doi":"10.1080/01480545.2025.2487865","DOIUrl":"https://doi.org/10.1080/01480545.2025.2487865","url":null,"abstract":"In this study, the protective effect of astaxanthin (AST) against cyclophosphamide (CP) induced adult rat heart damage was investigated. Eighteen rats were divided into 3 groups as Group 1: control, Group 2: cyclophosphamide and Group 3: cyclophosphamide + astaxanthin. The CP group, received a 200 mg/kg single dose intraperitoneal (i.p.) injection of CP on the seventh day of the experiment, while the control group received no treatment. For CP+AST group 25 mg/kg/day AST administered by oral gavage on days 1-7 and on the 7th day 200 mg/kg/day CP was administered by i.p injection. On the 8th day, the rats were sacrificed by exsanguination and the hearts were dissected. Histopathological examinations were performed by Hematoxylin&Eosin (H&E), Masson Trichrome and Periodic Acid-Schiff (PAS) staining methods; Annexin-V and Anti-NOX2/gp91phox analyzes were performed by flow cytometry. In histological evaluation of the CP Group; disruptions in cardiac histology and increased PAS(+) staining were observed. These findings were reduced in the CP+AST group compared to the CP group. According to flow cytometry measurements, there was an increase in Annexin-V and Anti-NOX2/gp91phox bound cells in the CP group. With the AST pretreatment, in the CP+AST group Annexin-V and Anti-NOX2/gp91phox bound cell level showed decrease. Based on our study's data, CP may alter cardiac histology and have a negative impact on apoptosis and oxidative damage processes. Astaxanthin may ameliorate these effects of CP on the heart. To enhance the assessment of this protective effect, we propose conducting future research utilizing varied dosages, application durations and advanced analytical techniques.","PeriodicalId":11333,"journal":{"name":"Drug and Chemical Toxicology","volume":" ","pages":"1-14"},"PeriodicalIF":2.1,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143794751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel synthesized, characterization and in vitro toxicological evaluation of amino acid-based-cerium oxide nanoparticles (CeO₂ NPs).

IF 2.1 4区医学

Drug and Chemical Toxicology Pub Date : 2025-03-31 DOI: 10.1080/01480545.2025.2484461

Sedanur Güngör, Buket Bakan

{"title":"Novel synthesized, characterization and in vitro toxicological evaluation of amino acid-based-cerium oxide nanoparticles (CeO2 NPs).","authors":"Sedanur Güngör, Buket Bakan","doi":"10.1080/01480545.2025.2484461","DOIUrl":"https://doi.org/10.1080/01480545.2025.2484461","url":null,"abstract":"Surface modifications improve the properties of nanomaterials and make them more appropriate for various applications. Various materials are used for surface modification of cerium nanoparticles (CeO2 NPs); however, there is no modification process of cerium with glutamic acid. This study focused on the preparation of L-glutamic acid-coated CeO2 NPs and reveal its potential toxicity depending on the characteristic properties. Characterization analysis was performed by Transmission electron microscopy (TEM), Fourier transform infrared (FT-IR), ZETA sizer/potential and X-ray diffraction (XRD). In vitro toxicities of CeO2 NPs and Glu-CeO2 NPs were investigated by using WST-1 (2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt), hemolysis and HET-CAM (Hen's egg-chorioallantoic membrane) tests. Both NPs-group were determined to be non- cytotoxic on L929 and MCF-10A cell lines, but slightly decrease the viability of A549 and MCF-7 cells after 24 exposure. Glu-CeO2 NPs increased cell migration in MCF-10A cell line but inhibited in A549 cells. In contrast to CeO2 NPs, Glu-CeO2 NPs reduced oxidative stress and hemolysis rate. CeO2 NP was caused lysis after 0.5nd min, while Glu-CeO2 NPs didn't exhibit any irritation effect. This is the first report on the synthesis of Glu-CeO2 NPs. This formulation may be used in drug delivery systems due to its potential to reduce toxicity of CeO2 NPs.","PeriodicalId":11333,"journal":{"name":"Drug and Chemical Toxicology","volume":" ","pages":"1-12"},"PeriodicalIF":2.1,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143751330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effects of alumina and polystyrene nanoparticles on global DNA methylation, antimicrobial peptides and intergenerational inheritance of Galleria mellonella.

IF 2.1 4区医学

Drug and Chemical Toxicology Pub Date : 2025-03-31 DOI: 10.1080/01480545.2025.2483970

Zülbiye Demirtürk, Fevzi Uçkan

{"title":"The effects of alumina and polystyrene nanoparticles on global DNA methylation, antimicrobial peptides and intergenerational inheritance of Galleria mellonella.","authors":"Zülbiye Demirtürk, Fevzi Uçkan","doi":"10.1080/01480545.2025.2483970","DOIUrl":"https://doi.org/10.1080/01480545.2025.2483970","url":null,"abstract":"The epigenetic and immunological effects of nanoparticles (NPs), which have started to be described as nano-pollutants today, are of great interest in living organisms. Particularly alumina (Al) and polystyrene (PS) are among the most produced NPs. Galleria mellonella larvae, an ideal model for the multi-generational effects of these NPs on global DNA methylation and the immune system, were used in the experiments. Al-NPs were bought, and PS-NPs were produced by the single emulsion solvent evaporation method. Al and PS-NPs were administered to larvae at different concentrations by changing only the water content in the diet. Global DNA methylation levels in the first and second generations were determined by HPLC. The expression levels of β-actin, transferrin, galiomycin, and p38 MAPK genes which constitute antimicrobial peptides, one of the humoral immune responses, were determined by RT-qPCR in two generations. The data obtained revealed that Al and PS-NPs increased global DNA methylation, and partially suppressed humoral immune responses. Furthermore, changes in genomic DNA methylation and immune-related gene expression levels induced by NPs in first generation larvae were found to be inherited by the next generation. Considering the importance of multigenerational epigenetic effects and changes in the immune system, our study results contribute to the literature and reveal the importance of such studies.","PeriodicalId":11333,"journal":{"name":"Drug and Chemical Toxicology","volume":" ","pages":"1-14"},"PeriodicalIF":2.1,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143751533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MiR-27b-3p ameliorates DOX-induced cardiotoxicity by suppressing myocardial inflammation and oxidative stress in mice and cardiomyocytes.

IF 2.1 4区医学

Drug and Chemical Toxicology Pub Date : 2025-03-31 DOI: 10.1080/01480545.2025.2481873

Ying Gao, Shujun Yang

{"title":"MiR-27b-3p ameliorates DOX-induced cardiotoxicity by suppressing myocardial inflammation and oxidative stress in mice and cardiomyocytes.","authors":"Ying Gao, Shujun Yang","doi":"10.1080/01480545.2025.2481873","DOIUrl":"https://doi.org/10.1080/01480545.2025.2481873","url":null,"abstract":"Doxorubicin (DOX), a chemotherapeutic drug used for cancer treatment, faces limitations in clinical use due to its cardiotoxicity. The study intended to investigate the effect of microRNA (miR)-27b-3p on DOX-induced cardiotoxicity. Quantitative polymerase chain reaction was conducted to identify the miR-27b-3p expression in cardiac tissues of 24 mice exposure to doxorubicin for 0-7days. To investigate the functions of miR-27b-3p, the remaining 40 mice were assigned into 4 experimental groups (n=10 per group): Control+miR-scramble, Control+miR-27b-3p, chronic heart failure (CHF) + miR-scramble, and CHF+miR-27b-3p. Specifically, C57BL/6J mice received a tail vein injection of adeno-associated viral 9 (AAV9)-miR-27b-3p/miR-scramble and/or intraperitoneal injection of 15mg/kg DOX. Echocardiography was used to measure basic cardiac function parameters. Hematoxylin-eosin and Sirius red staining were performed to assess cardiac structural changes and fibrotic areas. For cellular experiments, neonatal mouse cardiomyocytes were exposure to 5μg/ml DOX. The levels of inflammatory factors and oxidative stress indicators in cardiac tissues or cardiomyocytes were assessed by western blotting, enzyme-linked immunosorbent assay, or corresponding detection kits. The results showed that miR-27b-3p expression was downregulated in mouse cardiac tissues following DOX treatment. Overexpression of miR-27b-3p improved cardiac function and ameliorated pathological changes in mice. In addition, DOX-induced myocardial inflammation and oxidative stress were mitigated by miR-27b-3p overexpression both in vivo and in vitro. MiR-27b-3p negatively regulated the expression of four target genes (Plk2, Adora2b, Apaf1 and Nrk) in DOX-stimulated cardiomyocytes. In conclusion, miR-27b-3p ameliorates DOX-induced cardiac dysfunction and myocardial injury by inhibiting inflammation and oxidative stress.","PeriodicalId":11333,"journal":{"name":"Drug and Chemical Toxicology","volume":" ","pages":"1-16"},"PeriodicalIF":2.1,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143751328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Piribedil and thymol mitigate vancomycin-evoked nephrotoxicity in rats through modulation of Keap-1/Nrf2/HO-1 and NF-κB/Bax/caspase 3 signalings.

IF 2.1 4区医学

Drug and Chemical Toxicology Pub Date : 2025-03-26 DOI: 10.1080/01480545.2025.2481857

Rania Yahia, Gehad Gamal Hassan, Amira M Abo-Youssef, Heba M Mahmoud

{"title":"Piribedil and thymol mitigate vancomycin-evoked nephrotoxicity in rats through modulation of Keap-1/Nrf2/HO-1 and NF-κB/Bax/caspase 3 signalings.","authors":"Rania Yahia, Gehad Gamal Hassan, Amira M Abo-Youssef, Heba M Mahmoud","doi":"10.1080/01480545.2025.2481857","DOIUrl":"https://doi.org/10.1080/01480545.2025.2481857","url":null,"abstract":"Nephrotoxicity is a sign in which endogenous or exogenous toxicants have damaged the kidney-specific detoxification and excretion processes. Vancomycin (VAN) exposure mostly causes kidney damage and a loss of body homeostasis regulation. This study aimed to investigate the protective effects of piribedil and thymol and its basic mechanisms against nephrotoxicity caused by VAN. Randomly, the animals were categorized into six groups (n = 8). For 7 d, Group I only received vehicles, Group II received piribedil (5 mg/kg/once daily, i.p.), Group III received thymol (25 mg/kg/once daily, i.p), Group IV was administered a single daily dose of VAN (200 mg/kg, i.p.), VAN+ piribedil was administered to Group V, and VAN + thymol was administered to Group VI. The findings showed that piribedil or thymol improved renal function parameters by an increase in serum albumin level in parallel to a decrease in serum creatinine and blood urea nitrogen (BUN) levels in addition to decreased levels of KIM-1 and serum cystatin C. Furthermore, enhanced oxidative stress biomarkers as GSH, myeloperoxidase (MPO), and malondialdehyde (MDA) as well as tumor necrosis factor-alpha (TNF-α) and interleukin-1beta (IL-1β), indicators of inflammatory mediators, were markedly reduced compared to VAN group. Moreover, piribedil or thymol markedly improved the histopathological aberrations provoked by VAN, increased the Nrf-2 and HO-1 renal protein expressions and reduced VAN-induced elevation of Keap-1 protein expression. In addition, NF-kB, Bax, and caspase 3 expression levels were considerably declined after piribedil or thymol co-treatment. These findings revealed that co-administration of piribedil or thymol with VAN may be a sensible therapeutic approach for reducing renal intoxication caused by VAN.","PeriodicalId":11333,"journal":{"name":"Drug and Chemical Toxicology","volume":" ","pages":"1-16"},"PeriodicalIF":2.1,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143718341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oleuropein mitigates acetaminophen overdose-induced kidney injury in male rats by enhancing antioxidant defense and suppressing inflammatory and apoptotic pathways.

IF 2.1 4区医学

Drug and Chemical Toxicology Pub Date : 2025-03-26 DOI: 10.1080/01480545.2025.2483338

Zahra Sedghi, Ayat Kaeidi, Jalal Hassanshahi

{"title":"Oleuropein mitigates acetaminophen overdose-induced kidney injury in male rats by enhancing antioxidant defense and suppressing inflammatory and apoptotic pathways.","authors":"Zahra Sedghi, Ayat Kaeidi, Jalal Hassanshahi","doi":"10.1080/01480545.2025.2483338","DOIUrl":"https://doi.org/10.1080/01480545.2025.2483338","url":null,"abstract":"Acetaminophen (APAP) is a well-known analgesic, and antipyretic drug and its overdose or chronic consumption can lead to kidney damage. Oleuropein (OLE) exhibits various pharmacological properties. This study aimed to determine the possible therapeutic benefits of OLE in improving APAP-induced kidney injury. In this experimental study, 36 male Wistar rats were assigned to six groups (n = 6). The rats initially received a single dose of APAP (500 mg/kg) and then 1 hour later were treated with a single dose of OLE at 50, 100, and 200 mg/kg depending on their groups. 24 hours after treatment with OLE, various indicators including kidney biochemical tests, histopathological changes, oxidative stress markers, and anti-apoptotic and anti-inflammatory parameters were investigated in the renal tissue. OLE (100 mg/kg) significantly decreased serum creatinine, caspase-3, kidney tissue damage score (P < 0.05), malondialdehyde (MDA) (P < 0.01), and increased superoxide dismutase (SOD) and total antioxidant capacity (TAC) (P < 0.05) in the APAP + OLE 100 mg/kg group versus APAP group. Additionally, OLE (200 mg/kg) significantly reduced blood urea nitrogen (BUN), NF-κB, p53, Bax (p < 0.05), serum creatinine, TNF-α, caspase-3, kidney tissue damage score (p < 0.01), MDA, and Bax: Bcl-2 ratio (p < 0.001) in the APAP + OLE 200 mg/kg group versus APAP group. Also, OLE (200 mg/kg) significantly enhanced glutathione peroxidase (GPx), SOD, Bcl-2 (p < 0.05), and TAC (p < 0.01) in the APAP + OLE 200 mg/kg group contrasted to APAP group. However, OLE at 50 mg/kg didn't alter measured parameters. These findings demonstrate that OLE (200 mg/kg) could attenuate acetaminophen-induced kidney injury through its anti-oxidant, anti-inflammatory, and anti-apoptosis properties.","PeriodicalId":11333,"journal":{"name":"Drug and Chemical Toxicology","volume":" ","pages":"1-10"},"PeriodicalIF":2.1,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cardiotoxicity induced by xanthatin via activating apoptosis and ERS pathways in zebrafish.

IF 2.1 4区医学

Drug and Chemical Toxicology Pub Date : 2025-03-24 DOI: 10.1080/01480545.2025.2481863

Lixin Feng, Liyan Xu, Jing Huang, Yuxin Wang, Qing Xia, Jin Meng, Rongchun Wang, Kechun Liu

{"title":"Cardiotoxicity induced by xanthatin via activating apoptosis and ERS pathways in zebrafish.","authors":"Lixin Feng, Liyan Xu, Jing Huang, Yuxin Wang, Qing Xia, Jin Meng, Rongchun Wang, Kechun Liu","doi":"10.1080/01480545.2025.2481863","DOIUrl":"https://doi.org/10.1080/01480545.2025.2481863","url":null,"abstract":"Xanthatin, a sesquiterpene lactone compound, isolated from Chinese herb, Xanthium strumarium L, has various activities, including anti-inflammatory, anti-tumor, anti-ulcer effects. However, it has been less studied in terms of its toxicity, especially the potential toxicity on heart. This study is mainly aimed to assess the cardiotoxicity of xanthatin in vivo using zebrafish larva and in vitro using cardiomyocytes H9C2. The cardiotoxicity in zebrafish was assessed by the pericardial edema, blood flow dynamics, SV-BA distance, and sub-intestinal vein. The apoptosis was determined by AO staining, the blood red cell reduction and distribution was detected by O-dianisidine staining, histopathological evaluations were detected by HE staining. The anti-proliferative and pro-apoptotic activities in H9C2 cells were assessed by EdU staining and Hoechst 33342/PI double staining. The in vivo results showed that xanthatin caused cardiac malformations and dysfunctions, including decreased heart rate, reduced red blood cell count, hemodynamics, stroke volume, increased SV-BA distance and sub-intestinal vein congestion. Furthermore, apoptosis occurred in the heart of the zebrafish after xanthatin exposure. Additionally, cat, Mn-sod, chop, perk, and hspa5 related to oxidative stress and ERS also changed by xanthatin. Apoptotic genes caspase3 and caspase9 were also increased. Moreover, the in vitro results showed that xanthatin had proapoptotic and antiproliferative effects. To sum up, these results suggest that xanthatin has cardiotoxicity and the oxidative stress, ERS and apoptosis pathways are involved in the cardiotoxicity induced by xanthatin. This finding will be helpful for the better understanding of the potential cardiotoxicity of xanthatin and the underlying mechanism.","PeriodicalId":11333,"journal":{"name":"Drug and Chemical Toxicology","volume":" ","pages":"1-12"},"PeriodicalIF":2.1,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143691535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neuroprotective effects of gastrodin against bisphenol A induced-ADHD-like symptoms in rats.

IF 2.1 4区医学

Drug and Chemical Toxicology Pub Date : 2025-03-10 DOI: 10.1080/01480545.2025.2473472

Mohd Anas Saifi, Huma Khan, Mehjbeen Javed, Shahzad Ahmad, Zuber Khan, Anuradha Mangla, Garima Jindal, Suhel Parvez, Nidhi Agarwal, Sheikh Raisuddin

{"title":"Neuroprotective effects of gastrodin against bisphenol A induced-ADHD-like symptoms in rats.","authors":"Mohd Anas Saifi, Huma Khan, Mehjbeen Javed, Shahzad Ahmad, Zuber Khan, Anuradha Mangla, Garima Jindal, Suhel Parvez, Nidhi Agarwal, Sheikh Raisuddin","doi":"10.1080/01480545.2025.2473472","DOIUrl":"https://doi.org/10.1080/01480545.2025.2473472","url":null,"abstract":"Gastrodin (GAS) is a potent pharmaceutical compound extracted from the dried roots of a Chinese medicinal herb, Gastrodia elata Blume. It has been used as a neuroprotective treatment for a range of neurological disorders. Bisphenol A (BPA) has been implicated in the induction of attention-deficit hyperactivity disorder (ADHD)-like symptoms. We investigated the neuroprotective effects of GAS against BPA-induced ADHD-like symptoms in the rat model. Weanling male Wistar rats treated with BPA (50 µg/kg b.w. × 30 days per os) on completion of treatment were subsequently treated with GAS at two doses (30 and 60 mg/kg b.w. i.p. × 7 days). After 24 hours of completion of the treatment regimen, neurobehavioral parameters such as open field test (OFT), novel object recognition (NOR), and elevated plus maze (EPM) test were evaluated. Lipid peroxidation, reduced glutathione (GSH), monoamine oxidase (MAO) activity, and dopamine (DA) levels were measured in the cerebral cortex (CC) and hippocampus (HC) regions of the brain. Additionally, to asses astrocyte activatio the expression of glial fibrillary acidic protein (GFAP) was analyzed by immunostaining. Results show that GAS treatment ameliorated locomotory activity (OFT), memory dysfunction (NOR), and anxious (EPM) behavioral alterations in BPA-treated animals. GAS treatment also reduced lipid peroxidation, enhanced GSH, MAO activity, and DA levels, and reduced GFAP-positive cells in the CC and HC regions thus providing experimental evidence for a neuroprotective role for GAS against BPA-induced ADHD-like symptoms. GAS demonstrated potential preventive effects against BPA-induced ADHD-like symptoms in rats which highlights its therapeutic value.","PeriodicalId":11333,"journal":{"name":"Drug and Chemical Toxicology","volume":" ","pages":"1-14"},"PeriodicalIF":2.1,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143585065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cytotoxicity of methamphetamine exposure on Sertoli cells: a pilot study with implications for male infertility.

IF 2.1 4区医学

Drug and Chemical Toxicology Pub Date : 2025-03-04 DOI: 10.1080/01480545.2025.2471383

David Fisher, Omar Zabida, Sahar Abdul-Rasool, Chontrelle Willemse

{"title":"Cytotoxicity of methamphetamine exposure on Sertoli cells: a pilot study with implications for male infertility.","authors":"David Fisher, Omar Zabida, Sahar Abdul-Rasool, Chontrelle Willemse","doi":"10.1080/01480545.2025.2471383","DOIUrl":"https://doi.org/10.1080/01480545.2025.2471383","url":null,"abstract":"Methamphetamine (Meth), a psychoactive drug, has been shown to reduce testicular weight and decrease sperm count, indicating its potential role in contributing to male infertility. We therefore assessed Meth's effects (0.1-100 μM) on TM4 Sertoli cell viability, toxicity, and proliferation (trypan blue exclusion assay), mitochondrial activity (MA) (XTT assay), while transepithelial electrical resistance (TEER) was used to examine monolayer permeability. The acute study (only 24-hour Meth exposure) mimics recreational users and the chronic study, the Meth addicts who require daily doses (24-96 hours). Acute Meth treatment had minimal impact on TM4 Sertoli cell viability and toxicity, while chronic exposure resulted in reduced cell viability and increased toxicity in a dose-related manner. Acute exposure suppressed cell division at 72 hours, while chronic exposure suppressed cell division at both 72 and 96 hours. Long-term suppression of MA was observed for both acute and chronic Meth exposure (20 µM and 100 µM). Both acute and chronic Meth exposure affected permeability across the blood-testis barrier (BTB), which persisted for up to 96 hours. Given the pivotal role of Sertoli cells in spermatogenesis, our findings provide a two-pronged mechanism for Meth-induced male infertility and indicate that short-term exposure may have long-term effects on the germinal epithelium.","PeriodicalId":11333,"journal":{"name":"Drug and Chemical Toxicology","volume":" ","pages":"1-11"},"PeriodicalIF":2.1,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143540550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0