Drug and Chemical Toxicology最新文献_第2页

Protective effects of glycine against diclofenac-induced toxicity in isolated rat hepatocytes. 甘氨酸对离体大鼠肝细胞双氯芬酸毒性的保护作用。

IF 1.9 4区医学

Drug and Chemical Toxicology Pub Date : 2025-10-10 DOI: 10.1080/01480545.2025.2564433

Mostafa G Elkot, Hisham A Nematalla, Ihab T Abdel-Raheem, Asser I Ghoneim

{"title":"Protective effects of glycine against diclofenac-induced toxicity in isolated rat hepatocytes.","authors":"Mostafa G Elkot, Hisham A Nematalla, Ihab T Abdel-Raheem, Asser I Ghoneim","doi":"10.1080/01480545.2025.2564433","DOIUrl":"https://doi.org/10.1080/01480545.2025.2564433","url":null,"abstract":"Diclofenac (DIC) is a common cyclo-oxygenase inhibitor that has been linked to liver toxicity. Alternatively, glycine (GLY) has several beneficial actions, including direct cytoprotective, anti-inflammatory, anti-apoptotic, and antioxidant effects. Hence, this study investigated the ability of GLY to protect isolated rat hepatocytes against DIC-induced injury. Hepatocytes were isolated using a modified collagenase-based rat liver perfusion and digestion method. Assessment was conducted on trypan blue (TB) uptake, as well as, reduced glutathione (GSH), lipid peroxidation (LPO), nitric oxide (NO), nuclear factor kappa B (NF-κB), and tumor necrosis factor-alpha (TNF-α) levels. Caspase-3 activity was also measured. Pre-incubation of hepatocytes with GLY (40 mM) for 30 minutes prior to treatment with DIC (200 µM) significantly mitigated hepatocytotoxicity. Additionally, the DIC-depleted GSH level was restored following GLY pretreatment. Moreover, levels of LPO, NO, NF-κB, TNF-α, and caspase-3 activity were normalized and diminished relative to the DIC-intoxicated group. Therefore, GLY prevented DIC-induced hepatocyte toxicity, at least partly, by its cytoprotective, antioxidative, anti-inflammatory, and anti-apoptotic properties. Further research regarding the clinical pharmacologic and toxicologic effects of administering GLY to patients treated with high-dose DIC is thus recommended.","PeriodicalId":11333,"journal":{"name":"Drug and Chemical Toxicology","volume":" ","pages":"1-12"},"PeriodicalIF":1.9,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145273923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction. 修正。

IF 1.9 4区医学

Drug and Chemical Toxicology Pub Date : 2025-09-18 DOI: 10.1080/01480545.2025.2548103

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Oxidative stress and toxicity induced by copper and zinc oxide nanoparticles in liver and kidney tissues of male mice. 氧化铜和氧化锌纳米颗粒对雄性小鼠肝脏和肾脏组织的氧化应激和毒性作用。

IF 1.9 4区医学

Drug and Chemical Toxicology Pub Date : 2025-09-18 DOI: 10.1080/01480545.2025.2543425

Özge Temiz, Ferit Kargin, Hikmet Cogun, Özge Fırat

{"title":"Oxidative stress and toxicity induced by copper and zinc oxide nanoparticles in liver and kidney tissues of male mice.","authors":"Özge Temiz, Ferit Kargin, Hikmet Cogun, Özge Fırat","doi":"10.1080/01480545.2025.2543425","DOIUrl":"https://doi.org/10.1080/01480545.2025.2543425","url":null,"abstract":"Copper and Zinc Oxide Nanoparticles are widely used in pharmacy, cosmetics, agriculture and engineering fields according to their chemical and biological properties; their toxic effects on living systems up to the environment and humans are seen in physiological processes. The study included 60 male mice divided into 10 groups; 100 μl water as placebo in the control group, copper oxide (CuO-NP) and zinc oxide (ZnO-NP) nanoparticles and CuO-NP+ZnO-NP groups were administered oral gavage at different doses (1, 5 and 25 mg/kg/day) for 14 days to investigate the toxic effects on the tissues of male mice. Antioxidant enzyme activities glutathione-S-transferase (GST), catalase (CAT), superoxide dismutase (SOD) and oxidative damage parameters glutathione (GSH) and thiobarbituric acid reactive substance (TBARS) were measured in the tissues by spectrophotometric methods. Stress protein 70 (HSP70) and DNA oxidation (8-OHdG) levels were measured using ELISA methods. The exposure to CuO-NPs + ZnO-NPs, CuO-NPs, and ZnO-NPs resulted in changes in biochemical parameters in the liver and kidney tissues, with varying effects observed across the groups compared to the control. The most significant changes were observed in the CuO-NPs + ZnO-NPs group, including decreases in SOD, CAT activities, GSH levels and increases in GST activity, HSP70 and 8-OHdG levels. As the oxidative damage and biomolecular parameters stress protein and DNA oxidation were induced, which are consistent with the parameters of toxic effects in both examined tissues, the co-exposure to CuO-NPs and ZnO-NPs appears to suppress the antioxidant system, suggesting that these biomarkers may serve as potential indicators of tissue toxicity caused by nanoparticles.","PeriodicalId":11333,"journal":{"name":"Drug and Chemical Toxicology","volume":" ","pages":"1-12"},"PeriodicalIF":1.9,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145079891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of benzophenone-2 exposure on the reproductive outcomes in adult female mice. 二苯甲酮-2暴露对成年雌性小鼠生殖结果的影响。

IF 1.9 4区医学

Drug and Chemical Toxicology Pub Date : 2025-09-17 DOI: 10.1080/01480545.2025.2559816

Ahmed Lasaneya, Jogen Chandra Kalita

{"title":"Impact of benzophenone-2 exposure on the reproductive outcomes in adult female mice.","authors":"Ahmed Lasaneya, Jogen Chandra Kalita","doi":"10.1080/01480545.2025.2559816","DOIUrl":"https://doi.org/10.1080/01480545.2025.2559816","url":null,"abstract":"Benzophenone-2 (BP2) is considered a potential endocrine disruptor, but due to limited data availability, its specific impact on reproductive function is not fully understood. The current study investigated the in vivo toxic effect of BP2 in female mice at 50, 100, and 200 mg/kg body weight, which aimed to evaluate whether it can show an impact on reproductive estrous cycle, ovarian weight, biochemical parameters, histoarchitecture, and ovarian follicle count when administered daily for 7 and 21 days in female mice. This study tested the hypothesis that BP2 disrupts pregnancy parameters in mice. The data indicate that 7- and 21-day exposure to BP2 caused irregularities in different phases of the estrous cycle, such as significantly prolonged duration in estrus and significantly decreased duration in the diestrus phase. The effects of BP2 included elevated serum AST, ALT, cholesterol, and triglycerides. The percentage of different stages of developed, healthy follicles, and corpus luteum was significantly reduced, and atretic follicles increased in the treatment mice. Besides, BP2 altered prenatal fertility outcomes in pregnant mice. In conclusion, this study suggests that the BP2 exerts an anti-fertility effect on reproductive functions in adult female mice, highlighting the potential risks of reproductive issues caused in females exposed to BP2.","PeriodicalId":11333,"journal":{"name":"Drug and Chemical Toxicology","volume":" ","pages":"1-9"},"PeriodicalIF":1.9,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145074604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biological effects of epoxy resins on the human body: toxicity and allergic reactions. 环氧树脂对人体的生物效应：毒性和过敏反应。

IF 1.9 4区医学

Drug and Chemical Toxicology Pub Date : 2025-09-17 DOI: 10.1080/01480545.2025.2557405

Maria Zofia Lisiecka

{"title":"Biological effects of epoxy resins on the human body: toxicity and allergic reactions.","authors":"Maria Zofia Lisiecka","doi":"10.1080/01480545.2025.2557405","DOIUrl":"https://doi.org/10.1080/01480545.2025.2557405","url":null,"abstract":"This research focused on assessing the impact of epoxy resin components on human health and formulating measures to mitigate their adverse effects. The investigation highlighted the primary routes of exposure to toxic substances, including the inhalation of volatile compounds and direct contact with skin or mucous membranes. The most hazardous components were identified as epichlorohydrin, bisphenol A, and amine hardeners, which were associated with both immediate and long-term toxic effects, such as irritation, inflammatory responses, and the potential for tissue accumulation. Particular attention was directed to sensitization processes resulting from the interaction of epoxy resin constituents with proteins in the skin or mucous membranes, leading to allergic responses like contact dermatitis and systemic sensitization. Additionally, the study examined the influence of occupational settings on the toxicity of these substances, with a focus on measuring the levels of volatile organic compounds present in workplace environments. The study integrated a comprehensive review of scientific literature, experimental findings, and an assessment of toxicity, with a particular focus on industrial applications. Based on the findings, practical recommendations were proposed, including the implementation of personal protective equipment, the automation of production processes, the substitution of hazardous components with safer alternatives, and the establishment of monitoring systems to safeguard worker health. These outcomes are applicable in the manufacturing and utilization of epoxy resins, aiming to mitigate health hazards and enhance environmental sustainability.","PeriodicalId":11333,"journal":{"name":"Drug and Chemical Toxicology","volume":" ","pages":"1-11"},"PeriodicalIF":1.9,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145074662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Obesity aggravates neurotoxicity of bisphenol A in female rats via endoplasmic reticulum stress mediated death signals. 肥胖通过内质网应激介导的死亡信号加重雌性大鼠双酚A的神经毒性。

IF 1.9 4区医学

Drug and Chemical Toxicology Pub Date : 2025-09-17 DOI: 10.1080/01480545.2025.2555380

Anuradha Mangla, Mehjbeen Javed, Poonam Goswami, Garima Jindal, Iqra Mazahir, Suhel Parvez, Sheikh Raisuddin

{"title":"Obesity aggravates neurotoxicity of bisphenol A in female rats via endoplasmic reticulum stress mediated death signals.","authors":"Anuradha Mangla, Mehjbeen Javed, Poonam Goswami, Garima Jindal, Iqra Mazahir, Suhel Parvez, Sheikh Raisuddin","doi":"10.1080/01480545.2025.2555380","DOIUrl":"https://doi.org/10.1080/01480545.2025.2555380","url":null,"abstract":"Obesity is a chronic and multifactorial disease, in which activation of endoplasmic reticulum (ER) stress and resulting cell death have been widely implicated leading to the emergence of neurological diseases. Misfolded or unfolded proteins accumulation triggers ER stress, which modulates mitochondrial-associated death signals. Bisphenol A (BPA), an endocrine-disrupting chemical, is known to exert multiple deleterious effects on the brain. However, the influence of pre-established obesity in females on BPA-induced neurotoxicity remains poorly characterized. This study examines whether obesity aggravates the BPA toxicity in the cerebral cortex region of the brain. Rats were divided into five groups: control, HFD (high-fat diet), HFD + BPA, BPA, and thapsigargin (positive control). Obesity was induced by feeding 60% HFD (12 weeks), followed by chronic exposure to BPA (10 ppm in drinking water) for another 12 weeks. Thapsigargin (10 µg; 5 µg on either side) was given intracerebroventricularly 72 h before sacrifice. Transmission electron microscopy (TEM) revealed the deformed morphology of the ER and mitochondria. Expression levels of ER stress-associated proteins (BiP and CHOP) and genes (ATF4 and GADD34) were significantly higher (p < 0.05) in HFD + BPA rats compared to BPA alone. p-eif2α was significantly upregulated (p < 0.05) in all the treated rats as compared to the control. BPA-exposed obese rats had also shown a significantly increased ratio of apoptotic proteins Bax and Bcl2. Our findings suggest that the exacerbation of BPA toxicity through obesity can be attributed to the involvement of ER stress and the mitochondrial death signals it mediates.","PeriodicalId":11333,"journal":{"name":"Drug and Chemical Toxicology","volume":" ","pages":"1-12"},"PeriodicalIF":1.9,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145074624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Glyphosate in the Iberian Peninsula: Evaluating risks to Iberian wildlife. 草甘膦在伊比利亚半岛：评估对伊比利亚野生动物的风险。

IF 1.9 4区医学

Drug and Chemical Toxicology Pub Date : 2025-09-03 DOI: 10.1080/01480545.2025.2553870

Catarina Jota Baptista, Gonçalo Nogueira Marques, Luísa Lima Gonçalves, Ricardo Assunção, Mónica Martinez-Haro

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Metabolomic insights into rotenone-induced dysregulation of purine metabolism and impaired insulin secretion in murine pancreatic beta cells. 代谢组学洞察鱼藤酮诱导嘌呤代谢失调和小鼠胰腺β细胞胰岛素分泌受损。

IF 1.9 4区医学

Drug and Chemical Toxicology Pub Date : 2025-09-02 DOI: 10.1080/01480545.2025.2552761

Surachai Ngamratanapaiboon, Pracha Yambangyang, Phichanan Duchda, Jenyuk Lohwacharin, Watcharaporn Devakul Na Ayutthaya

{"title":"Metabolomic insights into rotenone-induced dysregulation of purine metabolism and impaired insulin secretion in murine pancreatic beta cells.","authors":"Surachai Ngamratanapaiboon, Pracha Yambangyang, Phichanan Duchda, Jenyuk Lohwacharin, Watcharaporn Devakul Na Ayutthaya","doi":"10.1080/01480545.2025.2552761","DOIUrl":"https://doi.org/10.1080/01480545.2025.2552761","url":null,"abstract":"Rotenone, an insecticide, and herbicide has been associated with various environmental and health concerns. This study investigates the molecular alterations in rotenone-treated murine pancreatic beta cells, using untargeted metabolomics based on liquid chromatography-mass spectrometry. We established a model mimicking rotenone toxicity in MIN6 cells and observed decreased insulin secretion despite no significant loss of cellular viability. Our untargeted metabolomics analysis revealed a decrease in 19 metabolites and an increase in 62 metabolites following exposure to rotenone. Mapping these changes onto metabolic pathways, we found that purine metabolism underwent significant alterations. Critical metabolites in this pathway, including adenine, adenosine monophosphate, guanine, and others, exhibited a substantial increase upon rotenone treatment. This study underscores the utility of untargeted metabolomics for investigating molecular alterations due to rotenone exposure. It further highlights rotenone's significant impact on purine metabolism, providing potential insights into the mechanisms of rotenone-associated diabetes risk.","PeriodicalId":11333,"journal":{"name":"Drug and Chemical Toxicology","volume":" ","pages":"1-8"},"PeriodicalIF":1.9,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144946761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of telomere length and shelterin genes in men and women leukocytes and their correlations with lipid peroxidation in sulfur mustard gas intoxication. 硫芥子气中毒中男性和女性白细胞端粒长度和庇护蛋白基因的表达及其与脂质过氧化的关系。

IF 1.9 4区医学

Drug and Chemical Toxicology Pub Date : 2025-09-02 DOI: 10.1080/01480545.2025.2553203

Leila Nasiri, Mohammad-Reza Vaez-Mahdavi, Tooba Ghazanfari, Hossein Hassanpour, Sussan Kaboudanian-Ardestani

{"title":"Expression of telomere length and shelterin genes in men and women leukocytes and their correlations with lipid peroxidation in sulfur mustard gas intoxication.","authors":"Leila Nasiri, Mohammad-Reza Vaez-Mahdavi, Tooba Ghazanfari, Hossein Hassanpour, Sussan Kaboudanian-Ardestani","doi":"10.1080/01480545.2025.2553203","DOIUrl":"https://doi.org/10.1080/01480545.2025.2553203","url":null,"abstract":"Sulfur mustard (SM), a chemical warfare agent, inflicts severe acute and chronic health effects. This study investigates the impact of SM-induced oxidative stress on telomere length (TL) and shelterin gene expression, which are crucial for telomere maintenance in exposed veterans. This study involved SM-exposed veterans and non-exposed controls. The SM-exposed group was divided into three subgroups based on exposure severity (severe, mild, and asymptomatic) and gender. Leukocyte TL, transcript of shelterin genes (TPP1, POT1, TIN2, TRF1, TRF2, RAP1), and plasma MDA were measured. TL was decreased in the SM-exposed group compared to the non-exposed group, while the MDA level was increased. The SM-exposed group showed lower expression of TIN2, TRF2, and the composite shelterin genes compared to the control group. In the SM-exposed subgroups, TL, TRF2 transcript, and composite shelterin gene expression were reduced compared to the non-exposed group, while the MDA levels were significantly increased. There are negative correlations between MDA and both TIN2/TRF2 expression and TL, and positive correlations between TL and composite shelterin gene expression. In the gender comparison, there were different effects of SM toxicity on TIN2, TPP1, TRF2, and the composite of shelterin gene expression between SM-exposed men and women. SM-exposed men had significantly higher MDA levels, while women showed no significant change. Also, there was no difference between non-exposed men and women. It is concluded that SM exposure increases lipid peroxidation, shortens telomeres, and alters shelterin genes in a gender-specific manner, suggesting accelerated biological aging as a delayed toxic effect.","PeriodicalId":11333,"journal":{"name":"Drug and Chemical Toxicology","volume":" ","pages":"1-11"},"PeriodicalIF":1.9,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144946719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Furan impairs cell function by inducing oxidative stress, DNA damage and apoptosis in mouse Sertoli cells in vitro. 呋喃在体外诱导小鼠 Sertoli 细胞氧化应激、DNA 损伤和细胞凋亡，从而损害细胞功能。

IF 1.9 4区医学

Drug and Chemical Toxicology Pub Date : 2025-09-01 Epub Date: 2024-12-16 DOI: 10.1080/01480545.2024.2437056

Yasemin Aydin, Yasemin Ulku Dikbasan, Banu Orta-Yilmaz

{"title":"Furan impairs cell function by inducing oxidative stress, DNA damage and apoptosis in mouse Sertoli cells in vitro.","authors":"Yasemin Aydin, Yasemin Ulku Dikbasan, Banu Orta-Yilmaz","doi":"10.1080/01480545.2024.2437056","DOIUrl":"10.1080/01480545.2024.2437056","url":null,"abstract":"Research on heat-induced food contaminants, such as furan, has shown its harmful effects on various systems. However, the impact of furan on Sertoli cells, a crucial male reproductive system cell, has not been studied. The investigation involved the treatment of furan to TM4 Sertoli cells at various concentrations (750, 1500, and 3000 µM) over a period of 24 h. This in vitro study determined that furan causes a decrease in Sertoli cell viability and an increase in lactate dehydrogenase activity, leading to cytotoxicity. Additionally, we observed an increase in MDA, one of the oxidative stress markers, in Sertoli cells, indicating that furan exposure leads to lipid peroxidation. It was determined that enzyme activities in the antioxidant defense system in Sertoli cells decreased after furan exposure. The findings indicate that furan induces oxidative damage in Sertoli cells by impairing the activity of antioxidant enzymes and promoting the production of ROS. This study discovered that furan triggers apoptosis in Sertoli cells by damaging DNA and altering the expression levels of apoptotic genes. Moreover, results suggest that furan causes cellular toxicity and apoptosis, leading to damage to Sertoli cells and thus causing male infertility.","PeriodicalId":11333,"journal":{"name":"Drug and Chemical Toxicology","volume":" ","pages":"1067-1079"},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142834585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0