水飞蓟素减轻大鼠氯化钴诱导的氧化还原失衡和心肾功能障碍。

IF 2.1 4区 医学 Q3 CHEMISTRY, MULTIDISCIPLINARY
Temitayo Olabisi Ajibade, Okezi Michael Ohore, Oluwaseun Olarenwaju Esan, Bisi Olajumoke Adeoye, Ayodele Stephen Ake, Moses Olusola Adetona, Omolola Victoria Awoyomi, Olumayowa Olawumi Igado, Taiwo Olaide Oyagbemi, Adewunmi Victoria Adeogun, Ademola Adetokunbo Oyagbemi, Temidayo Olutayo Omobowale, Oluwafemi Omoniyi Oguntibeju, Evaristus Nwulia, Momoh Audu Yakubu
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引用次数: 0

摘要

水飞蓟素是水飞蓟的提取物,传统上用于治疗几种疾病。本研究旨在评估水飞蓟素对氯化钴(CoCl2)诱导的大鼠心肾毒性的保护作用。40只大鼠随机分为4组,每组10只:对照组;CoCl2 300 mg/kg;CoCl2 + 100 mg/kg水飞蓟素;和水飞蓟素100毫克/公斤。所有给药均为口服。试验期(7 d)结束时,测定大鼠心脏和肾脏组织的血压参数、氧化应激标志物、抗氧化防御状态、肾功能、组织病理学和免疫组织化学表达。水飞蓟素显著(p 2)诱导血压参数、抗氧化剂和氧化应激标志物、血尿素氮和肌酐的改变。未给予水飞蓟素处理的CoCl2暴露大鼠的组织病理学检查显示心肌有炎症细胞浸润和肾脏出血,而对照组和水飞蓟素组则没有这些病变。在没有水飞蓟素处理的CoCl2暴露的大鼠心脏组织中,心肌肌钙蛋白I和基质金属蛋白酶2 (MMP-2)的免疫组化表达增加。与对照组和水飞蓟素组相比,CoCl2未处理组胱抑素C免疫组化表达升高,而血管紧张素转换酶2 (ACE2)免疫组化表达减弱。综上所述,水飞蓟素能够有效减轻CoCl2对大鼠心脏和肾脏组织的毒性作用,其机制可能是水飞蓟素能够正向调节心脏和肾脏系统的内源性抗氧化剂活性,中和活性氧。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Silymarin attenuates cobalt chloride-induced redox imbalance and cardio-renal dysfunctions in rats.

Silymarin is an extract of Silybum marianum that is used traditionally for the treatment of several diseases. This study sought to evaluate the protective effects of silymarin on cobalt chloride (CoCl2)-induced cardio-renal toxicities in rats. Forty rats were randomly divided into four groups of 10 rats each: control; 300 mg/kg CoCl2; CoCl2 + 100 mg/kg silymarin; and 100 mg/kg silymarin only. All administrations were done orally. At the end of the experimental period (seven days), blood pressure parameters, markers of oxidative stress, antioxidant defense status, renal function test, histopathology and immunohistochemical expressions were evaluated on the heart and kidney tissues. Silymarin significantly (p < 0.05) altered CoCl2-induced alterations in blood pressure parameters, antioxidants and markers of oxidative stress, blood urea nitrogen and creatinine. Histopathological evaluation revealed area of infiltration of the myocardium by inflammatory cells and hemorrhages in the kidney of rats exposed to CoCl2 without silymarin treatment, but these lesions were absent in the control and silymarin groups. Increased immunohistochemical expression of cardiac troponin I and matrix metalloproteinase-2 (MMP-2) was observed in the cardiac tissues of rats exposed to CoCl2 without silymarin treatment. The immunohistochemical expression of cystatin C was heightened, while that of angiotensin-converting enzyme 2 (ACE2) was attenuated in the CoCl2 untreated group compared with the control and silymarin groups. In conclusion, silymarin effectively mitigated the toxic effects of CoCl2 on the heart and kidney tissues of rats due to its ability to positively modulate the activities of endogenous antioxidants and neutralize reactive oxygen species in cardiac and renal systems.

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来源期刊
Drug and Chemical Toxicology
Drug and Chemical Toxicology 医学-毒理学
CiteScore
6.00
自引率
3.80%
发文量
99
审稿时长
3 months
期刊介绍: Drug and Chemical Toxicology publishes full-length research papers, review articles and short communications that encompass a broad spectrum of toxicological data surrounding risk assessment and harmful exposure. Manuscripts are considered according to their relevance to the journal. Topics include both descriptive and mechanics research that illustrates the risk assessment implications of exposure to toxic agents. Examples of suitable topics include toxicological studies, which are structural examinations on the effects of dose, metabolism, and statistical or mechanism-based approaches to risk assessment. New findings and methods, along with safety evaluations, are also acceptable. Special issues may be reserved to publish symposium summaries, reviews in toxicology, and overviews of the practical interpretation and application of toxicological data.
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