Deciphering the genotoxic and cytotoxic properties of teicoplanin: a combined laboratory and computational investigation.

IF 2.1 4区医学 Q3 CHEMISTRY, MULTIDISCIPLINARY

Drug and Chemical Toxicology Pub Date : 2025-05-08 DOI:10.1080/01480545.2025.2502446

Ahmet Ali Berber, Işıl Deniz Aliravci, Nihan Akinci Kenanoğlu, Şefika Nur Demir

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引用次数: 0

Abstract

In this study, the mutagenicity and carcinogenicity of the teicoplanin antibiotic were first investigated using the Vega Hub and Toxtree software through in silico prediction. The cytotoxic and genotoxic effects were evaluated using in vitro assays, including the mitotic index (MI), micronucleus (MN), nuclear division index (NDI), and Comet Assay (CA) in human lymphocytes. In the in vitro studies, both 24-hour and 48-hour exposures were conducted for MI, and teicoplanin significantly decreased MI compared to the control at all concentrations. In addition, a significant increase was detected in the MN frequency compared to the negative control at all concentrations. In the Comet assay, tail length significantly increased compared to the control at all concentrations except for 5.6 µg/mL, while tail moment and comet tail intensity significantly increased at all concentrations compared to the control. In conclusion, within the concentration range used in this study, teicoplanin was found to have cytotoxic and genotoxic effects.

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破译替柯planin的基因毒性和细胞毒性：一项联合实验室和计算研究。

本研究首次利用Vega Hub和Toxtree软件，通过计算机预测对teicoplanin抗生素的致突变性和致癌性进行了研究。通过体外实验，包括人淋巴细胞的有丝分裂指数（MI）、微核（MN）、核分裂指数（NDI）和彗星测定（CA），评估了细胞毒性和基因毒性效应。在体外研究中，对心肌梗死进行了24小时和48小时的暴露，与所有浓度的对照组相比，替柯planin显著降低了心肌梗死。此外，在所有浓度下，与阴性对照相比，检测到MN频率显著增加。在Comet实验中，除5.6µg/mL外，在所有浓度下，尾长都比对照组显著增加，而尾矩和彗尾强度在所有浓度下都比对照组显著增加。总之，在本研究使用的浓度范围内，发现teicoplanin具有细胞毒性和基因毒性作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Drug and Chemical Toxicology 医学-毒理学

CiteScore

6.00

自引率

3.80%

发文量

审稿时长

3 months

期刊介绍： Drug and Chemical Toxicology publishes full-length research papers, review articles and short communications that encompass a broad spectrum of toxicological data surrounding risk assessment and harmful exposure. Manuscripts are considered according to their relevance to the journal. Topics include both descriptive and mechanics research that illustrates the risk assessment implications of exposure to toxic agents. Examples of suitable topics include toxicological studies, which are structural examinations on the effects of dose, metabolism, and statistical or mechanism-based approaches to risk assessment. New findings and methods, along with safety evaluations, are also acceptable. Special issues may be reserved to publish symposium summaries, reviews in toxicology, and overviews of the practical interpretation and application of toxicological data.