Dose-Response最新文献_第6页

In Vitro Antioxidant and Inhibitory Study of Picrorhiza kurroa (Kutki), Syzygium aromaticum (Loung), Lawsonia inermis (Henna), Rheum emodi (Revand Chini), Curcuma longa (Haldi) Against Lipid Per-Oxidation in Mice Brain and Liver. 苦木（Kutki）、香附（Loung）、无叶劳松（Henna）、大黄（Revand Chini）、姜黄（Haldi）对小鼠脑和肝脂质过氧化的体外抗氧化和抑制作用研究。

IF 2.5 4区医学

Dose-Response Pub Date : 2023-10-25 eCollection Date: 2023-10-01 DOI: 10.1177/15593258231210431

Farooq Hassan, Asmat Ullah Khan, Syed Zahoor Ul Hassan Zaidi, Madiha Khan Niazi, Muhammad Amjed Ismail

{"title":"In Vitro Antioxidant and Inhibitory Study of Picrorhiza kurroa (Kutki), Syzygium aromaticum (Loung), Lawsonia inermis (Henna), Rheum emodi (Revand Chini), Curcuma longa (Haldi) Against Lipid Per-Oxidation in Mice Brain and Liver.","authors":"Farooq Hassan, Asmat Ullah Khan, Syed Zahoor Ul Hassan Zaidi, Madiha Khan Niazi, Muhammad Amjed Ismail","doi":"10.1177/15593258231210431","DOIUrl":"https://doi.org/10.1177/15593258231210431","url":null,"abstract":"The aerobic organisms not only need oxygen for survival, but oxygen is also fundamentally malignant to the aerobic organism on the grounds of free radical generation and their affiliation with free oxidative stress. This study was done to evaluate the antioxidant and protective properties of P kurroa, S aromaticum, L inermis, R emodi, and C longa against lipid peroxidation induced by different pro-oxidants. The aqueous extracts of these medicinal plants showed inhibition against thiobarbituric acid reactive species (TBARS) induced by different pro-oxidants (10 mM FeSO4 and 5 mM sodium nitroprusside) in the brain and liver of mice. Moreover, the free radical scavenging activities of the extracts were evaluated by the scavenging of the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical. L inermis, S aromaticum, and R emodi showed higher inhibitory effects, which could be attributed to their significantly reduced ability and free radical scavenging activities. Therefore, the oxidative stress in the brain and liver could be potentially managed or prevented by the dietary intake of L inermis, S aromaticum, and R emodi plants, which justifies the use of these plants in various degenerative diseases. C longa and P kurroa showed relatively weak antioxidant activities.","PeriodicalId":11285,"journal":{"name":"Dose-Response","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10605699/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71411133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fabrication, Properties, and Stability of Oregano Essential Oil and Sodium Alginate-Based Wound-Healing Hydrogels. 牛至精油和海藻酸钠基创伤愈合水凝胶的制备、性质和稳定性。

IF 2.5 4区医学

Dose-Response Pub Date : 2023-10-09 eCollection Date: 2023-10-01 DOI: 10.1177/15593258231204186

Dure Najaf Iqbal, Asia Ashraf, Arif Nazir, Samar Z Alshawwa, Munawar Iqbal, Naveed Ahmad

{"title":"Fabrication, Properties, and Stability of Oregano Essential Oil and Sodium Alginate-Based Wound-Healing Hydrogels.","authors":"Dure Najaf Iqbal, Asia Ashraf, Arif Nazir, Samar Z Alshawwa, Munawar Iqbal, Naveed Ahmad","doi":"10.1177/15593258231204186","DOIUrl":"https://doi.org/10.1177/15593258231204186","url":null,"abstract":"The wound dressings fabricated by polymers and oregano essential oil (OEO) can be very effective as a hydrogel. The current study has been focused on fabricating the hydrogel membranes of oregano oil encapsulated as an antibacterial agent into sodium alginate (SA) solution by solvent casting method and then evaluated the antibacterial, antioxidant activity, and physicochemical performance of SA/OEO-based polymeric membranes. The polymeric interactions, surface morphology, water absorption capability, thermal stability, and encapsulation efficiency were investigated by FT-IR, SEM, swelling ratio, DSC, and encapsulation efficiency. The percentage encapsulation efficiency of essential oil was 40.5%. FTIR validated the presence of molecular interaction between individual components. SEM images showed a rough and porous appearance for hydrogel membranes. Moreover, DSC showed that the fabricated membranes were thermally stable. The inclusion of more content OEO decreased swelling ratios. The antioxidant test was carried out by DPPH assay and antibacterial test through disc diffusion method against microbes. The results revealed that membranes containing the highest content of OEO had more excellent antioxidant and antibacterial efficacy. Therefore, the polymeric membranes of sodium alginate loaded with oregano essential oil can be employed as an effective wound-healing candidate.","PeriodicalId":11285,"journal":{"name":"Dose-Response","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/41/f3/10.1177_15593258231204186.PMC10563497.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41194545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dose Dependent Effect of Sulfamethoxazole on Inhibiting K_ATP Channel of Mouse Pancreatic β Cell. 磺胺甲恶唑对小鼠胰腺β细胞KATP通道的抑制作用。

IF 2.5 4区医学

Dose-Response Pub Date : 2023-09-28 eCollection Date: 2023-07-01 DOI: 10.1177/15593258231203611

Hiroshi Ogata, Shigeki Kitamura, Makoto Fujiwara, Masaru Shimizu, Chengbo Tan, Songji Zhao, Yuko Maejima, Kenju Shimomura

{"title":"Dose Dependent Effect of Sulfamethoxazole on Inhibiting KATP Channel of Mouse Pancreatic β Cell.","authors":"Hiroshi Ogata, Shigeki Kitamura, Makoto Fujiwara, Masaru Shimizu, Chengbo Tan, Songji Zhao, Yuko Maejima, Kenju Shimomura","doi":"10.1177/15593258231203611","DOIUrl":"https://doi.org/10.1177/15593258231203611","url":null,"abstract":"Sulfamethoxazole (SMX) is widely used as an antibiotic in the clinical application with side effects of hypoglycemia. This is because SMX contains the sulfonamide structure, which closes ATP-sensitive potassium (KATP) channels and induces insulin secretion. However, there are no detail reports that measure the effective dose that can close KATP channels and induce insulin secretion. In this study, whole-cell patch clamp recording was utilized to measure the effect of SMX on KATP channel activity on pancreatic β cells. Also, the static incubation assay with mice islets was assessed to measure the insulin secretion capacity of SMX. SMX was shown to inhibit the KATP channel in pancreatic β cell membrane and induce insulin secretion in relatively high concentration. The half maximal inhibitory concentration (IC50) for KATP channel activity of SMX was .46 ± .08 mM. It was also shown that a near IC50 concentration of SMX (.5 mM) was able to nearly fully block the KATP channel when simultaneously applied with low concentration sulfonylurea, tolbutamide (.01 mM). Our present data provide important information for the clinical use of SMX to treat infection in diabetic patients using sulfonylureas.","PeriodicalId":11285,"journal":{"name":"Dose-Response","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/b6/b5/10.1177_15593258231203611.PMC10540586.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41170947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Toxic Potential of Crude Extract From Cassava Cortex Containing Scopoletin and Cyanide on Wistar Rats and Broilers. 含有Scopoletin和氰化物的木薯皮粗提取物对Wistar大鼠和肉鸡的毒性。

IF 2.5 4区医学

Dose-Response Pub Date : 2023-09-22 eCollection Date: 2023-07-01 DOI: 10.1177/15593258231203587

Youchahou Ndam Njankouo, Maximilienne Ascension Nyegue, Ibrahim Njingou, Paul Fewou Moundipa, Pauline Mounjouenpou

{"title":"Toxic Potential of Crude Extract From Cassava Cortex Containing Scopoletin and Cyanide on Wistar Rats and Broilers.","authors":"Youchahou Ndam Njankouo, Maximilienne Ascension Nyegue, Ibrahim Njingou, Paul Fewou Moundipa, Pauline Mounjouenpou","doi":"10.1177/15593258231203587","DOIUrl":"https://doi.org/10.1177/15593258231203587","url":null,"abstract":"The present work aimed to evaluate acute and sub-acute toxicity of cortex cassava crude extract containing scopoletin and cyanide. The limit test method of OECD at 2000 mg/kg was used to determine the LD50 of the crude extract in female Wistar rats, and the sub-acute toxicity was conducted in broilers by incorporating their feed with the cortex powder at 2 mg, 4 mg and 8 mg of powder per 100 g of standard food, corresponding to MIC, 2MIC and 4MIC: those concentrations, respectively, corresponded to .07 μg, .14 μg and .28 μg of scopoletin and 2.8 μg, 5.6 μg and 11.2 μg of cyanide. An oral LD50 greater than 2000 mg/kg was obtained, and the effective antifungal dose was non-toxic. No changes were observed in the rate of biochemical parameters and relative weight of the liver, kidneys and brain of broilers. Any damages were not observed after the histopathological exams of these organs regardless the diet considered, compared to the control group. All results ranging from efficacy and safety of cassava cortex extract containing scopoletin and cyanide allowed us to consider it as biopesticide for staple food preservation against post-harvest losses.","PeriodicalId":11285,"journal":{"name":"Dose-Response","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/6c/a5/10.1177_15593258231203587.PMC10517615.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41124613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Radioactive ¹²⁵I Seed Inhibits Cell Migration and Invasion and Promotes Apoptosis by Inactivating the VEGFR2 Signaling Pathway in Cholangiocarcinoma. 放射性125I种子通过失活VEGFR2信号通路抑制胆管癌细胞迁移和侵袭并促进细胞凋亡

IF 2.5 4区医学

Dose-Response Pub Date : 2023-07-01 DOI: 10.1177/15593258231187348

Jun Luo, Jiaping Zheng, Hongxiang Yao, Binbing Wang, Zhewei Zhang, Guoliang Shao

{"title":"Radioactive 125I Seed Inhibits Cell Migration and Invasion and Promotes Apoptosis by Inactivating the VEGFR2 Signaling Pathway in Cholangiocarcinoma.","authors":"Jun Luo, Jiaping Zheng, Hongxiang Yao, Binbing Wang, Zhewei Zhang, Guoliang Shao","doi":"10.1177/15593258231187348","DOIUrl":"https://doi.org/10.1177/15593258231187348","url":null,"abstract":"Objectives: To investigate the potential mechanisms of 125I seed implantation therapeutic treatment on inactivating the VEGFR2/PI3K/AKT pathway in cholangiocarcinoma.Methods: The human cholangiocarcinoma cell lines HCCC-9810 and HuCCT1 were purchased for in vitro studies. The BALB/c nude mice were obtained for in vivo studies. The proliferation of cells was detected by CCK-8, colony formation, and BrdU staining. The migration and invasion of cells were determined by wound healing assay and Transwell assay, respectively. Hematoxylin and eosin staining was utilized for histological evaluation. Protein expression was determined by western blotting and immunohistochemistry.Results: Compared with the control group, .6 mCi group and .8 mCi group inhibited cholangiocarcinoma cells proliferation, invasion, migration, and promoted apoptosis, the protein expression of p-VEGFR2, VEGFR2, PI3K, p-AKT/AKT, cyclin B1, cyclin A, CDK1, and Bcl-2 was decreased. Similar results were obtained from in vitro experiments. However, when VEGF is overexpressed, the inhibitory effect of .8 mCi was partially significantly reversed on cholangiocarcinoma cells. The in vivo studies further confirmed the inhibitory effects of .6 mCi group and .8 mCi group on cholangiocarcinoma.Conclusion: 125I seed irradiation could inhibit cholangiocarcinoma cells proliferation, migration, and invasion and promote apoptosis through inactivation of the VEGFR2/PI3K/AKT signaling pathway.","PeriodicalId":11285,"journal":{"name":"Dose-Response","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/c4/56/10.1177_15593258231187348.PMC10328048.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10300412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prospective Nutraceutical Effects of Cinnamon Derivatives Against Insulin Resistance in Type II Diabetes Mellitus-Evidence From the Literature. 肉桂衍生物对II型糖尿病胰岛素抵抗的前瞻性营养作用——来自文献的证据

IF 2.5 4区医学

Dose-Response Pub Date : 2023-07-01 DOI: 10.1177/15593258231200527

Saima Naz Mohsin, Faiza Saleem, Ayesha Humayun, Afifa Tanweer, Ambreen Muddassir

{"title":"Prospective Nutraceutical Effects of Cinnamon Derivatives Against Insulin Resistance in Type II Diabetes Mellitus-Evidence From the Literature.","authors":"Saima Naz Mohsin, Faiza Saleem, Ayesha Humayun, Afifa Tanweer, Ambreen Muddassir","doi":"10.1177/15593258231200527","DOIUrl":"https://doi.org/10.1177/15593258231200527","url":null,"abstract":"Apart from advances in pharmaceutical antidiabetic agents, efforts are being made toward hypoglycemic agents derived from natural sources. Cinnamon has been reported to have significant benefits for human health, particularly as an anti-inflammatory, antidiabetic, and anti-hypertriglyceridemic agent. The phytochemicals in cinnamon can be extracted from different parts of plant by distillation and solvent extraction. These chemicals help in decreasing insulin resistance and can act against hyperglycemia and dyslipidemia, inflammation and oxidative stress, obesity, overweight, and abnormal glycation of proteins. Cinnamon has shown to improve all of these conditions in in vitro, animal, and/or human studies. However, the mechanism of action of active ingredients found in cinnamon remains unclear. The current review presents the outstanding ability of cinnamon derivatives to control diabetes by various pathways modulating insulin release and insulin receptor signaling. It was also found that the type and dosage of cinnamon as well as subject characteristics including drug interactions are likely to affect the response to cinnamon. Future research directions based on this review include the synergistic usage of various cinnamon derivatives in managing and/or preventing diabetes and possible other relevant chronic diseases.","PeriodicalId":11285,"journal":{"name":"Dose-Response","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ce/bb/10.1177_15593258231200527.PMC10494518.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10244527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Valorization of Baker Yeast Industry Waste in Agriculture by Improving Germination and Growth of Barley and Pea. 通过促进大麦和豌豆的发芽和生长，发酵酵母工业废弃物在农业中的价值。

IF 2.5 4区医学

Dose-Response Pub Date : 2023-07-01 DOI: 10.1177/15593258231198974

Nadhem Aissani, Rania Aissani, Makrem Ghidaoui, Ferjeni Zouidi, Hichem Sebai

{"title":"Valorization of Baker Yeast Industry Waste in Agriculture by Improving Germination and Growth of Barley and Pea.","authors":"Nadhem Aissani, Rania Aissani, Makrem Ghidaoui, Ferjeni Zouidi, Hichem Sebai","doi":"10.1177/15593258231198974","DOIUrl":"https://doi.org/10.1177/15593258231198974","url":null,"abstract":"Industrial waste still present an environmental danger for the nature and survival of all living beings. Among these toxic products, the focus has been on liquid effluents from the baker's yeast industry that cause real environmental problems mainly due to their pollutant load and the release of unpleasant odors. In order to minimize these hazards and to take advantage of these wastes for the sake of our environment, the present work consists on valorizing effluents from the baker's yeast industry on barley (Hordeum vulgare) and pea (Pisum sativum), two important agricultural products of Tunisian north-west. Results showed that this waste is characterized by its richness in organic matter, and the presence of proteins traces with high chemical and biochemical oxygen demand (COD and BOD5) values. Diluted effluent at a dose of 2.5 mg/g significantly improves germination of both plant seeds by germination index (GI) calculation, to reach a maximum of 190 ± 17% and 150 ± 14% for barley and pea, respectively. In fertigation experiment, the use of a lower dose of .62 mg/g of diluted effluent promotes plant length to reach 52 ± 4 cm and 45 ± 1.4 cm, respectively, for H. vulgare and P. sativum. Gas chromatography coupled to mass spectrometry (GC-MS) analysis after derivatization showed significant enhancement of auxin production in pea treated with .62 mg/g of cream compared to control with a concentration of 10.60 ± .81 and 8.16 ± .43 ng/gFW, respectively. In another experiment, the irrigation of pea plants with furfural, as major compound of cream, promotes length and auxin production to reach 9.89 ± .56 ng/gFW for a furfural dose of .31 mg/g. This leads us to valorize baker's yeast effluent as an environment-friendly natural product in pea and barley agricultural and give insight to its mode of action.","PeriodicalId":11285,"journal":{"name":"Dose-Response","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/c4/cb/10.1177_15593258231198974.PMC10475235.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10297399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pharmacological Activities and In-Silico Studies of Bioactive Compounds Identified in Organic Fractions of the Methanolic Extract of Citrullus Colocynthis. 甜瓜醇提物有机组分的药理活性及生物活性化合物的计算机合成研究。

IF 2.5 4区医学

Dose-Response Pub Date : 2023-07-01 DOI: 10.1177/15593258231187357

Haseeb Akram Sindhu, Muhammad Afzal, Izzah Shahid

{"title":"Pharmacological Activities and In-Silico Studies of Bioactive Compounds Identified in Organic Fractions of the Methanolic Extract of Citrullus Colocynthis.","authors":"Haseeb Akram Sindhu, Muhammad Afzal, Izzah Shahid","doi":"10.1177/15593258231187357","DOIUrl":"https://doi.org/10.1177/15593258231187357","url":null,"abstract":"Medicinal plants have been extensively exploited for their immense pharmacological and immune-supporting potential. Fruit of Citrullus colocynthis has several active secondary metabolites such as phenolics, flavonoids, and essential oils that are used in traditional medicines as antidiabetic, anti-inflammatory, antioxidant, and antimicrobial agents. In this study, phytoconstituents in organic fractions (n-hexane, chloroform, and ethyl acetate) of the methanolic extract of C. colocynthis were analyzed and identified by FT-IR, HPLC, and GC-MS analysis. Ethyl acetate fraction showed the highest antioxidant scavenging (76 ± .769%) and anti-inflammatory (40 ± .473%) activities at the concentration of 3 mg/mL. Similarly, antidiabetic effect was measured by inhibition of α-amylase where, ethyl acetate fraction (77 ± .844%) exhibited the highest antidiabetic activity. Among all organic fractions, ethyl acetate exhibited strong antimicrobial potential followed by n-hexane and chloroform fractions against selected pathogenic bacteria. Various concentrations of the ethyl acetate extract were tested in-vivo for cytotoxicity and results indicated minor morphological changes in liver cells including ballooning, fatty droplets, and slight accumulation of extracellular matrix even at concentrations of 400 mg/kg. In-silico study showed that stigmasta-7,16-dien-3-ol had a strong interaction with COX-1 and COX-2 to reduce inflammation. The abovementioned results indicate the pharmacological strengths of C. colocynthis to fight several diseases.","PeriodicalId":11285,"journal":{"name":"Dose-Response","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/70/c6/10.1177_15593258231187357.PMC10331210.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10300888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Olea europaea L. Leaf Extract Alleviates Fibrosis Progression and Oxidative Stress Induced by Bleomycin on a Murine Model of Lung Fibrosis. 油橄榄叶提取物减轻博来霉素诱导的肺纤维化小鼠模型的纤维化进程和氧化应激。

IF 2.5 4区医学

Dose-Response Pub Date : 2023-07-01 DOI: 10.1177/15593258231200972

Sana Bahri, Anouar Abidi, Afef Nahdi, Raed Abdennabi, Mona Mlika, Ridha Ben Ali, Saloua Jameleddine

{"title":"Olea europaea L. Leaf Extract Alleviates Fibrosis Progression and Oxidative Stress Induced by Bleomycin on a Murine Model of Lung Fibrosis.","authors":"Sana Bahri, Anouar Abidi, Afef Nahdi, Raed Abdennabi, Mona Mlika, Ridha Ben Ali, Saloua Jameleddine","doi":"10.1177/15593258231200972","DOIUrl":"https://doi.org/10.1177/15593258231200972","url":null,"abstract":"In this study, we aim to investigate the effect of industrial Olea europaea L. leaf extract (OLE) against bleomycin (BLM)-induced pulmonary fibrosis (PF) in rats. Male Wistar rats were treated with a single intratracheal injection of BLM (4 mg/kg) and a daily intraperitoneal injection of OLE (10, 20, and 40 mg/kg) for 4 weeks. Results of HPLC and LC-MS analysis revealed a large amount of oleuropein (15.43%/DW) in OLE. BLM induced apparent damage of lung architecture with condensed collagen bundles, increased lipid peroxidation which has been deduced from malondialdehyde (MDA) levels: (.9 ± .13 vs .25 ± .12 nmol/mg protein) and hydroxyproline content (.601 ± .22 vs .154 ± .139 mg/g of lung tissue) and decreased catalase (CAT) (5.93.10-5 ± 4.23.10-5 vs 6.41.10-4 ± 2.33.10-4 μmol/min/mg protein) and superoxide dismutase (SOD) (28.73 ± 3.34 vs 50.13 ± 2.1 USOD/min/mg protein) levels compared to the control. OLE treatment (40 mg/kg) stabilized MDA content (.32 ± .15 and .27 ± .13 vs .9 ± .13 nmol/mg protein), normalized SOD (61.27 ± 13.37 vs 28.73 ± 3.34 USOD/min/mg protein), and CAT (5.2.10-4 ±1.8.10-4 vs 5.93.10-5 ± 4.23.10-5 μmol/min/mg protein) activities and counteracted collagen accumulation and hydroxyproline content (.222 ± .07 vs .601 ± .22 mg/g of lung tissue) in the lung parenchyma. Finally, OLE might have a potent protective effect against PF by regulating oxidative parameters and attenuating collagen deposition, due to the existence of large amount of bioactive phenolic molecules.","PeriodicalId":11285,"journal":{"name":"Dose-Response","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/7c/65/10.1177_15593258231200972.PMC10475267.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10297397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Scutellarin Inhibits Glioblastoma Growth in a Dose-dependent Manner by Suppressing the p63 Signaling Pathway. 黄芩素通过抑制p63信号通路以剂量依赖性方式抑制胶质母细胞瘤的生长。

IF 2.5 4区医学

Dose-Response Pub Date : 2023-07-01 DOI: 10.1177/15593258231197101

Yongjie Chen, Wenlan Li

{"title":"Scutellarin Inhibits Glioblastoma Growth in a Dose-dependent Manner by Suppressing the p63 Signaling Pathway.","authors":"Yongjie Chen, Wenlan Li","doi":"10.1177/15593258231197101","DOIUrl":"https://doi.org/10.1177/15593258231197101","url":null,"abstract":"Although scutellarin has been extensively investigated, its effects on glioma are unclear. This study intended to reveal this regulation and the underlying mechanisms. The U251, M059K, and SF-295 cell lines were treated with gradient concentrations of scutellarin and then IC50 was calculated. SF-295 cells selected for subsequent procedures were treated with four concentrations of scutellarin. Then, proliferation, apoptosis, and cell cycle, as well as the protein and mRNA expression of significantly differentially expressed genes identified by next-generation sequencing (NGS), were examined. The curative effect of scutellarin was validated by 5-FU as the positive control. Scutellarin inhibited proliferation and induced apoptosis and G2/M cell cycle arrest in the SF-295 cell line in a dose-dependent manner. The effect of scutellarin was similar to but significantly weaker than the effect of 5-FU. The NGS results showed that genes associated with anti-apoptosis signaling pathways were significantly reduced after treatment. The Western blotting results indicated that the expressions of TP63/BIRC3/TRAF1/Bcl-2 were reduced in a dose-dependent manner, as well as the mRNA levels determined by qRT‒PCR. Our original conclusion revealed that scutellarin may inhibit glioma growth in a dose-dependent manner via the p63 signaling pathway which may provide a potential medicine for glioma chemotherapy.","PeriodicalId":11285,"journal":{"name":"Dose-Response","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/88/96/10.1177_15593258231197101.PMC10467202.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10307677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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