Dose-Response最新文献_第7页

Exposure to Cadmium Telluride Quantum Dots and Gene Expression Profile of Huh-7 Hepatocellular Carcinoma Cell Line. 碲化镉量子点暴露与Huh-7肝癌细胞系基因表达谱

IF 2.5 4区医学

Dose-Response Pub Date : 2023-07-01 DOI: 10.1177/15593258231185457

Hani Alothaid, Mashael R Al-Anazi, Arwa A Al-Qahtani, Dilek Colak, Azeez Yusuf, Mohammed S Aldughaim, Ali M Mahzari, Mahmoud M Habibullah, Saud Alarifi, Saad Alkahtani, Ahmed A Al-Qahtani

{"title":"Exposure to Cadmium Telluride Quantum Dots and Gene Expression Profile of Huh-7 Hepatocellular Carcinoma Cell Line.","authors":"Hani Alothaid, Mashael R Al-Anazi, Arwa A Al-Qahtani, Dilek Colak, Azeez Yusuf, Mohammed S Aldughaim, Ali M Mahzari, Mahmoud M Habibullah, Saud Alarifi, Saad Alkahtani, Ahmed A Al-Qahtani","doi":"10.1177/15593258231185457","DOIUrl":"https://doi.org/10.1177/15593258231185457","url":null,"abstract":"Nanoparticles have shown promising potential for efficient drug delivery, circumventing biological interferences like immunological and renal clearance and mechanical and enzymatic destruction. However, a handful of research papers have questioned the biomedical use of metal-based nanoparticles like cadmium telluride quantum dots (CdTe-QDs) for their cytotoxic, genotoxic, and carcinogenic potential. Herein, we examined the effects of CdTe-QD NPs on gene expression profile of hepatocellular carcinoma (Huh-7) cell line. Huh-7 cells were treated with CdTe-QD NPs (10 μg/ml for 6, 12, and 24 hours, and 25 μg/ml for 6 and 12 hours), and transcriptomic analysis was performed using microarray to evaluate the global gene expression profile. Differential expressed genes (DEGs) were observed for both the doses (10 and 25 μg/ml) of CdTe-QD NPs at different time points. Gene ontology (GO) analysis revealed that genes involved in molecular function of cell cycle, organizational injury and abnormalities, cell death and survival, gene expression, cancer, organismal survival, and cellular development were differentially expressed. Overall, we have demonstrated differential expression of several genes, involved in maintaining cell survival, metabolism, and genome integrity. These findings were confirmed by RT-qPCR study for some canonical pathway genes signifying possible implication in NP toxicity-mediated cell survival and inhibition of cell death.","PeriodicalId":11285,"journal":{"name":"Dose-Response","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/69/cf/10.1177_15593258231185457.PMC10467207.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10305355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Condiment Recipes Lead to Reduced Generation of Carcinogenic Polycyclic Aromatic Hydrocarbons in Duck Variety Meat During Charcoal Grilling 调味料配方可减少木炭烧烤过程中鸭肉致癌物多环芳烃的生成

4区医学

Dose-Response Pub Date : 2023-07-01 DOI: 10.1177/15593258231203212

Gulshan Shahid, Muhammad Yousaf, Haseeb Anwar, Imran Mukhtar, Abdul Basit

{"title":"Condiment Recipes Lead to Reduced Generation of Carcinogenic Polycyclic Aromatic Hydrocarbons in Duck Variety Meat During Charcoal Grilling","authors":"Gulshan Shahid, Muhammad Yousaf, Haseeb Anwar, Imran Mukhtar, Abdul Basit","doi":"10.1177/15593258231203212","DOIUrl":"https://doi.org/10.1177/15593258231203212","url":null,"abstract":"The current study aimed to evaluate the impact of charcoal grilling in the generation of various polycyclic aromatic hydrocarbons in the tissues of 5 different organs (leg, chest, wings, liver, and heart) of falcated ducks ( Mareca falcata) before and after pasting them with different condiment recipes (R1, R2, R3, and R4). All condiment-pasted and control samples before/after charcoal grilling were pursued in RP-HPLC for quantification of unknown PAHs. Tissues from grilled raw leg meat of the control sample showed significantly higher ( P ≤ .05) concentration (42.40 ng/g) of overall PAHs as compared to all other grilled samples. However, overall PAHs concentration (9.99 ng/g) in charcoal grilled tissues of leg meat pasted with R4 condiment recipe was decreased 76.43% significantly ( P ≤ .05) as compared to all other recipes of pasted charcoal grilled samples. All PAHs, particularly naphthalene, fluorene, phenanthrene, and acenaphthalene were decreased significantly ( P ≤ .05) to none detectable level in all tissue samples when grilled after treating with R4 condiment recipe. All condiment recipes reduced total PAHs level below MRL’s set by the international guidelines. Recipe R4, a rich source of antioxidants, significantly neutralized and reduced the generation of PAHs in duck leg meat tissue sample during wood charcoal grilling.","PeriodicalId":11285,"journal":{"name":"Dose-Response","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135807062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential In Vivo Effects on Cancer Models by Recorded Magnetic Signals Derived From a Healing Technique. 通过记录一种治疗技术产生的磁信号对癌症模型产生不同的体内效应。

IF 2.3 4区医学

Dose-Response Pub Date : 2023-06-02 eCollection Date: 2023-04-01 DOI: 10.1177/15593258231179903

William Bengston, Paul Cizdziel, Akane Tanaka, Hiroshi Matsuda

{"title":"Differential In Vivo Effects on Cancer Models by Recorded Magnetic Signals Derived From a Healing Technique.","authors":"William Bengston, Paul Cizdziel, Akane Tanaka, Hiroshi Matsuda","doi":"10.1177/15593258231179903","DOIUrl":"10.1177/15593258231179903","url":null,"abstract":"Previous research on \"healing-with-intent\" has reasonably demonstrated the validity of the phenomenon at least when a human healer is present and involved. However, in order for healing to be adopted into more conventional therapies, it must be able to be made scalable. The present study tests the effects of a scalable recording of the Bengston Healing Method on 3 cancer models. BalbC mice engrafted with 4T1 breast cancer cells, C57BL mice with melanoma B16 cells, and C3H mice with bladder MBT-2 wells were exposed to a recording of healing intent for 4 hours/day for approximately 1 month. In the breast cancer model, there was significant tumor suppression and a reduction of anemia marker HCT in treated vs control mice. In the melanoma model, there were no significant differences except for a reduction in platelet count among the treated mice. For unknown reasons, tumor growth never became evident in the bladder cancer model. While the effects of the recording seem to vary by model, there appears reason to pursue scalable delivery systems in multiple models and with multiple doses.","PeriodicalId":11285,"journal":{"name":"Dose-Response","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2023-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/60/e0/10.1177_15593258231179903.PMC10265331.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10351457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of Concern: "Optimization of Thymoquinone-Loaded Self-Nanoemulsion for Management of Indomethacin-Induced Ulcer". 关注表达："优化胸腺喹酮负载自纳米乳液用于治疗吲哚美辛诱发的溃疡"。

IF 2.5 4区医学

Dose-Response Pub Date : 2023-05-29 eCollection Date: 2023-04-01 DOI: 10.1177/15593258231175716

引用次数: 0

MCNPX Estimation of Photoneutron Dose to Eye Voxel Anthropomorphic Phantom From 18 MV Linear Accelerator. 18 MV直线加速器对眼体素拟人化幻影的光子中子剂量MCNPX估计

IF 2.5 4区医学

Dose-Response Pub Date : 2023-04-01 DOI: 10.1177/15593258231169807

Ali Aa Alghamdi

{"title":"MCNPX Estimation of Photoneutron Dose to Eye Voxel Anthropomorphic Phantom From 18 MV Linear Accelerator.","authors":"Ali Aa Alghamdi","doi":"10.1177/15593258231169807","DOIUrl":"https://doi.org/10.1177/15593258231169807","url":null,"abstract":"The dose due to photoneutron contamination outside the field of irradiation can be significant when using high-energy linear accelerators. The eye is a radiation-sensitive organ, and this risk increases when high linear energy transfer neutron radiation is involved. This study aimed to provide a fast method to estimate photoneutron dose to the eye during radiotherapy. A typical high-energy linear accelerator operating at 18 MV was simulated using the Monte Carlo N-Particle Transport Code System extended version (MCNPX 2.5.0). The latest International Atomic Energy Agency photonuclear data library release was integrated into the code, accounting for the most known elements and isotopes used in typical linear accelerator construction. The photoneutron flux from a 5 × 5 cm2 field size was scored at the treatment table plane and used as a new source for estimating the absorbed dose in a high-resolution eye voxel anthropomorphic phantom. In addition, common shielding media were tested to reduce the photoneutron dose to the eye using common shielding materials. Introducing a 2 cm thickness of common neutron shielding medium reduced the total dose received in the eye voxel anthropomorphic phantom by 54%. In conclusion, individualized treatment based on photoneutron dose assessment is essential to better estimate the secondary dose inside or outside the field of irradiation.","PeriodicalId":11285,"journal":{"name":"Dose-Response","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ee/fa/10.1177_15593258231169807.PMC10107973.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9383945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of Brown Micro-Algae Synergies With Low Dose γ-Radiation Against Chronic Hepatitis Induced by D-Galactosamine in Rats. 褐微藻配合低剂量γ辐射对d -半乳糖胺所致大鼠慢性肝炎的协同作用评价。

IF 2.5 4区医学

Dose-Response Pub Date : 2023-04-01 DOI: 10.1177/15593258231169405

Salma Elsaman, Sawsan M Elsonbaty, Fatma S M Moawed, Marwa G A Hegazy

{"title":"Evaluation of Brown Micro-Algae Synergies With Low Dose γ-Radiation Against Chronic Hepatitis Induced by D-Galactosamine in Rats.","authors":"Salma Elsaman, Sawsan M Elsonbaty, Fatma S M Moawed, Marwa G A Hegazy","doi":"10.1177/15593258231169405","DOIUrl":"https://doi.org/10.1177/15593258231169405","url":null,"abstract":"Introduction: Hepatic inflammation is considered key driver of hepatic tissue impairment.We aimed to explore the interaction of Halamphora coffeaeformis (Amph.) with low dose ionizing γ radiation (γR) exposure against D-galactosamine (D-GaIN)-induced chronic hepatitis in Albino rats.Methods: Chronic hepatitis was induced with single dose of D-GalN (400 mg/kg BW i.p.). Rats received 400 mg Amph/kg BW daily by gastric gavage concomitant with .25 Gy γ-R. Liver oxidative stress and inflammatory status were assessed. Gene expression levels of signal transducer and activator of transcription 3 (STAT3) and nuclear factor kappa B (NFKB) were estimated by q-PCR. D-Galactosamine injection significantly encouraged hepatic oxidative damage and inflammatory disturbance accompanied with improved intercellular adhesion molecule-1 level (ICAM-1).Results: messenger RNA gene expression levels of STAT3 and NF-kB were expressively higher in D-GaIN-treated animals. Histopathological examination supported results. Interestingly, Amph treatment with γ-radiation (γ-R) subjection displayed significant improvement of oxidative and inflammatory status along with controlled signaling molecular factors which was supported by amended histological structure of induced liver hepatitis.Conclusion: Results conclude the efficacious control of liver hepatitis progression by dual collaboration of Amph. with low dose γ-R via control of vital growth signaling factors linked with inflammation thru anti-inflammation, antioxidative and anti-proliferative activities.","PeriodicalId":11285,"journal":{"name":"Dose-Response","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ee/8c/10.1177_15593258231169405.PMC10107994.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9383948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective Effect of Trillium tschonoskii Maxim Components Against Glutamate-Induced SH-SY5Y Cells Damage Through Regulating Apoptosis. 兆子成分通过调节细胞凋亡对谷氨酸诱导的SH-SY5Y细胞损伤的保护作用

IF 2.5 4区医学

Dose-Response Pub Date : 2023-04-01 DOI: 10.1177/15593258231169585

Yanwen Li, Xingnan Yue, Shuo Wang, Pengyue Li, Man Zhang, Kuokui Song, Xiulan Huang, Zhiyong Li

{"title":"Protective Effect of Trillium tschonoskii Maxim Components Against Glutamate-Induced SH-SY5Y Cells Damage Through Regulating Apoptosis.","authors":"Yanwen Li, Xingnan Yue, Shuo Wang, Pengyue Li, Man Zhang, Kuokui Song, Xiulan Huang, Zhiyong Li","doi":"10.1177/15593258231169585","DOIUrl":"https://doi.org/10.1177/15593258231169585","url":null,"abstract":"Context Among the Tujia people, the root or rhizome of Trillium tschonoskii Maxim.in Bull.Acad (TTM) is considered a miraculous herb for headaches. Previous studies have shown ethyl acetate extract (TTM1) can protect SH-SY5Y cells against glutamate injury. Objective This study clarified TTM1’s mechanism against glutamate-induced cell damage, focusing on the regulation of apoptosis. The compounds were separated, identified, and performed molecular docking with pro-apoptotic proteins. Materials and Methods SH-SY5Y cells were treated with glutamate (2 mM) for 12 hour, and the effect of TTM1 (2.5, 5, 10, and 20 μg/mL) was evaluated with MTT and LDH release assays, taking EGb761(40 μg/mL) as a control. Cell apoptosis was detected with Hoechst 33258 and Annexin V-FITC and measurements of intracellular calcium and caspase-3. The major components were separated and identified by LCMS-IT-TOF and NMR, then the proapoptotic activity of TTM1 was confirmed by molecular docking method. Results TTM1 protected SH-SY5Y cells by resisting apoptosis, TTM1 (10 and 20 μg/mL) decreased apoptotic bodies and nuclear fragments, increased the proportion of normal cells to 68.38 ± 5.63% and 92.80 ± .88%, decreased VA cells to 4.30 ± .76% and 3.58 ± .45% and caspase-3 to .365 ± .034 and .344 ± .047 ng/mL.TTM1 (10 μg/mL) decreased intracellular free calcium to 2.77 ± .40. Polyphyllin VI and pennogenin 3-O-β-chacotrioside were identified in TTM1 at 15.04% and 2.84%, and had potential anti-apoptosis activities. Discussion and Conclusions Folk records of TTM for headache may be related to its anti-apoptosis of nerve cells. Identification and content determination of index components based on effective extract provides research paradigms for rare and endangered ethnic plants.","PeriodicalId":11285,"journal":{"name":"Dose-Response","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/79/95/10.1177_15593258231169585.PMC10240882.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10290713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Important Methodological Concern Regarding the Article "Effect of Leg Half-Squat Training With Blood Flow Restriction Under Different External Loads on Strength and Vertical Jumping Performance in Well-Trained Volleyball Players": A Letter to the Editor. 关于《不同外负荷下限制血流的腿半深蹲训练对训练有素的排球运动员力量和垂直跳跃性能的影响》一文的重要方法学关注:致编辑的一封信。

IF 2.5 4区医学

Dose-Response Pub Date : 2023-04-01 DOI: 10.1177/15593258231173494

Nicholas Rolnick, Okan Kamis

{"title":"Important Methodological Concern Regarding the Article \"Effect of Leg Half-Squat Training With Blood Flow Restriction Under Different External Loads on Strength and Vertical Jumping Performance in Well-Trained Volleyball Players\": A Letter to the Editor.","authors":"Nicholas Rolnick, Okan Kamis","doi":"10.1177/15593258231173494","DOIUrl":"https://doi.org/10.1177/15593258231173494","url":null,"abstract":"We would like to commend Wang et al 1 on their investigation into the 8-week chronic training effects of blood ﬂ ow restriction (BFR) on trained volleyball players. Novel to the ﬁ eld was the comparison between low-load BFR (30% 1-repetition maximum, RM) and heavy loads (70% 1-RM) (HL) with and without BFR applied at an estimated 50% arterial occlusion pressure (AOP). BFR was prescribed in a commonly rec-ommended ﬁ xed scheme (30 repetitions followed by 3 sets of 15) 2 while HL strength training was performed for 4 sets of 8 repetitions. Results indicate that HL strength training with BFR performed better in thigh muscle strength and jumping performance than low-load BFR exercise and in some measures exhibited non-signi ﬁ cant improvements over HL strength training. The results are surprising given previous research has indicated no additional bene ﬁ t with the addition of BFR to HL on muscle strength measures 3 although one study did show improvements of performance in rugby athletes. 4 Nonetheless, the results of this study provide prelim-inary support for the use of BFR during HL strength training to elicit signi ﬁ cant gains in strength and jumping performance — factors thought to be important in improving volleyball performance. However, we want to highlight a signi ﬁ cant methodological limitation that impacts the interpretation of the study. We agree that utilizing a limb circumference equation is an appropriate surrogate for determining AOP when using single-chambered bladder systems capable of achieving full arterial","PeriodicalId":11285,"journal":{"name":"Dose-Response","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/0c/b1/10.1177_15593258231173494.PMC10141283.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9761971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dose Dependent Effects of Aqueous Extract of Garcinia cambogia Desr. Against Letrozole Induced Polycystic Ovarian Syndrome in Female Adult Rats With Possible Mechanisms Exploration. 黄藤水提物的剂量依赖性研究。来曲唑诱导雌性成年大鼠多囊卵巢综合征的作用机制探讨。

IF 2.5 4区医学

Dose-Response Pub Date : 2023-04-01 DOI: 10.1177/15593258231169381

Saba Rana, Liaqat Hussain, Uzma Saleem, Muhammad Asif, Arslan H Lodhi, Muhammad Q Barkat, Muhammad Riaz, Alina Jamil

{"title":"Dose Dependent Effects of Aqueous Extract of Garcinia cambogia Desr. Against Letrozole Induced Polycystic Ovarian Syndrome in Female Adult Rats With Possible Mechanisms Exploration.","authors":"Saba Rana, Liaqat Hussain, Uzma Saleem, Muhammad Asif, Arslan H Lodhi, Muhammad Q Barkat, Muhammad Riaz, Alina Jamil","doi":"10.1177/15593258231169381","DOIUrl":"https://doi.org/10.1177/15593258231169381","url":null,"abstract":"Background: Polycystic ovarian syndrome (PCOS) is an endocrine metabolic disorder of women.Purpose: This study aimed to explore the potential of aqueous extract of Garcinia cambogia Desr. (AEGC) in PCOS.Methodology: The HPLC was used to determine the phytoconstituents present in Garcinia cambogia. Thirty adult female albino rats were divided into 6 groups: Normal control (NC) disease Control (PCOS; letrozole 1 mg/kg), plant extract (AEGC 100, 300, 500 mg/kg) and standard (metformin; 20 mg/kg). Disease was confirmed by vaginal smear cytology. After 10 weeks, animals were euthanized, ovaries dissected for histopathology, blood collected for hormonal and biochemical analysis.Results: HPLC analysis showed the presence of phenolic contents; chlorogenic acid, gallic acid, coumaric acid while flavonoid contents were quercetin, kaempferol, and rutin. After treatment, there was dose dependent reduction of weight, ovarian cysts, improvement of follicle growth. DPPH radical scavenging percentage was 67.89%. Hormonal analysis showed a significant improvement (P < .05) in follicle stimulating hormone (FSH), estrogen, and progesterone while a reduction in testosterone, luteinizing hormone (LH) and insulin level. Antioxidant enzymatic markers were significantly (P < .05) increased. Lipid profile and LFTs were also improved.Conclusions: The study validated the potential of Garcinia cambogia in the management of PCOS.","PeriodicalId":11285,"journal":{"name":"Dose-Response","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/90/c0/10.1177_15593258231169381.PMC10103256.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9316200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Radiation Protection of Polydatin Against Radon Exposure Injury of Epithelial Cells and Mice. 聚丹素对氡照射上皮细胞及小鼠的辐射防护作用。

IF 2.5 4区医学

Dose-Response Pub Date : 2023-04-01 DOI: 10.1177/15593258231172271

Huiqin Chen, Fajian Luo, Huisheng Song, Huiqiang Long, Na Chen, Liang Sun, Fengmei Cui, Jun Wan, Yu Tu

{"title":"Radiation Protection of Polydatin Against Radon Exposure Injury of Epithelial Cells and Mice.","authors":"Huiqin Chen, Fajian Luo, Huisheng Song, Huiqiang Long, Na Chen, Liang Sun, Fengmei Cui, Jun Wan, Yu Tu","doi":"10.1177/15593258231172271","DOIUrl":"https://doi.org/10.1177/15593258231172271","url":null,"abstract":"Radon exposure is significantly associated with lung cancer. Radon concentration is currently reduced mainly by physical methods, but there is a lack of protective drugs or biochemical reagents for radon damage. This study aimed to explore the protective effect of polydatin (PD) on the radon-exposed injury. The results showed that PD can significantly reduce ROS level, raise SOD activity, weaken the migration ability, increase E-cad, and decrease mesenchymal cell surface markers (FN1, Vimentin, N-cad, α-SMA, and Snail) in radon-exposed epithelial cells. In vivo, PD increased the mice weight, promoted SOD activity, and decreased MDA content, the number of bullae, pulmonary septum thickness, lung collagenous fibers, and mesenchymal cell surface markers. Furthermore, PD inhibited p-PI3K, p-AKT, and p-mTOR expression. Compared with directly adding PD on radon-exposed cells, adding PD before and after radon exposure could more obviously improve the adhesion of radon-exposed cells, significantly alleviate the migration ability, and more significantly reduce mesenchyme markers and p-AKT and p-mTOR. These results indicate that PD can reduce oxidative stress, weaken epithelial-mesenchymal transition (EMT) and lung fibrosis in radon-exposed cells/mice, and have good radiation protection against radon injury. The mechanism is related to the inhibition of the PI3K/AKT/mTOR pathway.","PeriodicalId":11285,"journal":{"name":"Dose-Response","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/37/5b/10.1177_15593258231172271.PMC10134130.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9392791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1