Dose-Response最新文献

{"title":"Ameliorative Potential of Pumpkin Seed Oil (<i>Cucurbita pepo</i> L.) Against Tramadol-Induced Oxidative Stress.","authors":"Ezebuilo U Ekpono, Ejike D Eze, Afodun M Adam, Udu A Ibiam, Orji U Obasi, Josiah E Ifie, Ejike U Ekpono, Esther U Alum, Sana Noreen, Chinaza G Awuchi, Patrick M Aja","doi":"10.1177/15593258241226913","DOIUrl":"10.1177/15593258241226913","url":null,"abstract":"<p><strong>Background of the study: </strong>The increase in the therapeutic use of tramadol in the management of moderate to severe pains in some disease conditions and its unregulated access has led to its associated toxicity and there is little or no information on the protection against its associated toxicity.</p><p><strong>Aim of the study: </strong>Considering the medicinal value of pumpkin seed oil, its availability, and neglected use, it becomes necessary to evaluate the possible potential of the seed oil in tramadol-induced oxidative stress in Wister Albino rats.</p><p><strong>Methods of the study: </strong>This study used fifty-six (56) albino rats to determine the impact of <i>Cucurbita pepo</i> seed oil (CPSO) on tramadol-induced oxidative stress. The rats were grouped into 7. After a week of acclimatization, rats in group 1 (normal control) had access to water and food, while rats in group 2 received 5 mL/Kg (b.w) of normal saline. 100 mg/kg of tramadol (TM) was delivered to groups 3-6 to induce toxicity. The third group (TM control) received no treatment, whilst the other 3 groups (TM-CPSO treatment groups) received 5, 2.5, and 1.5 mL/Kg of CPSO, respectively. Group 7 received only 5 mL/kg CPSO (CPSO group). Similarly, groups 2 through 7 had unrestricted access to food and water for 42 days and received treatments via oral intubation once per day. Indicators of oxidative stress were discovered in the brain homogenate.</p><p><strong>Results: </strong>TM toxicity was demonstrated by a considerable increase (<i>P</i> < .05) in the brain MDA level and a significant drop (<i>P</i> < .05) in the brain GSH level, as well as a significant reduction (<i>P</i> < .05) in GPx, catalase, SOD, GST, and quinone reductase activities.</p><p><strong>Conclusion: </strong>The dose-dependent delivery of CPSO was able to restore not only the activity but also the concentrations of the altered markers.</p>","PeriodicalId":11285,"journal":{"name":"Dose-Response","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10793191/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139485263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical Properties of Crataegus azarolus Berries Decoction Extract and Evaluation of its Protective Activity Against Acetic Acid-Induced Ulcerative Colitis in Rats","authors":"Houcem Sammari, S. Jedidi, Houcine Selmi, Mourad Jridi, Ala Ayari, H. Sebai","doi":"10.1177/15593258241226890","DOIUrl":"https://doi.org/10.1177/15593258241226890","url":null,"abstract":"The present study aims to evaluate the protective effect of Crataegus azarolus berries decoction extract (CAB-DE) against acetic acid-induced ulcerative colitis as well as the mechanisms implicated in such protection. Adult male Wistar rats were separated into seven groups: Control (H2O), acetic acid (AA), AA + various doses of CAB-DE (100, 200, and 400 mg/kg, b.w.,p.o.), and AA + sulfasalazine (100 mg/kg, b.w.,p.o.) or gallic acid (50 mg/kg, b.w.,p.o.) during 10 days. All rats were kept fasting overnight and ulcerative colitis was induced by rectal infusion of AA (300 mg kg−1, b.w.) (3%, v/v, 5 mL kg−1 b.w), for 30 s. The colon was rapidly excised and macroscopically examined to measure ulcerated surfaces and the ulcer index. In vitro, we found that CAB-DE exhibited a high antioxidant activity against DPPH radical (IC50 = 164.17 ± 4.78 μg/mL). In vivo, pretreatment with CAB-DE significantly protected the colonic mucosa against AA-induced damage by stimulating mucus secretion, reducing ulcer index as well as histopathological changes. Also, CAB-DE limited the oxidative status induced by AA in the colonic mucosa, as assessed by MDA and H2O2 increased levels and the depletion of both enzymatic activities and non-enzymatic levels. In addition, AA intoxication increased iron and calcium levels in colonic mucosa and plasma, while CAB-DE pretreatment regulated all intracellular mediators deregulation and significantly reduced inflammatory markers such as CRP (1.175 ± .04 ─ .734 ± .06 μg/dl) and ALP (161.53 ± 5.02 ─ 98.60 ± 4.21 UI/L) levels. We suggest that CAB-DE protected against AA-induced ulcerative colitis due in part to its antioxidant and anti-inflammatory properties.","PeriodicalId":11285,"journal":{"name":"Dose-Response","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139440720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Letter re: \"On Geraily et al - Comments\".","authors":"Michael Patrick Russell Waligórski","doi":"10.1177/15593258231225914","DOIUrl":"10.1177/15593258231225914","url":null,"abstract":"","PeriodicalId":11285,"journal":{"name":"Dose-Response","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10775722/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139416656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Glycogen Synthase Kinase Pathway for Assessing the Antidepressant-like Effect of Glucosamine as a Radioprotector in Rats: Behavioral and Biochemical Studies.","authors":"Mai H Mekkawy, Heba M Karam, Marwa A Mohamed, Dina M Lotfy","doi":"10.1177/15593258231217845","DOIUrl":"https://doi.org/10.1177/15593258231217845","url":null,"abstract":"<p><p>Radiotherapy is a very important tool in the treatment of cancer; nevertheless, its side effects are a hindrance to its use. The present study is designed to evaluate glucosamine effects against radiation-induced brain oxidative stress and depression-like effect in rats. Four groups of female Wister rats were used as control, irradiated (4 × 2 Gy), glucosamine (1 g/kg P.O), and glucosamine + irradiated group. The behavioral responses are estimated. The brain hippocampi of the rats are separated to evaluate oxidative stress biochemical parameters and glycogen synthase kinase pathway in addition to the biogenic amines. Irradiation exposure led to disturbances in the behavioral assessments (forced swimming test, light-dark box, and open field test) and a significant decrease in brain GSH, neurotransmitters (serotonin, norepinephrine, and dopamine), phosphatidylinositol 3 kinase (PI3K), and phosphorylated protein kinase-B (p-AKT) levels. Additionally, MDA and ROS levels increased significantly post-irradiation along with the phosphorylated glycogen synthase kinase (p-GSK3). Glucosamine administration before irradiation caused improvement in the behavioral valuations and the biochemical parameters in the brain as well. Glucosamine might be used as a radioprotector to improve brain function and as an antidepressant drug. It could be promising as a future therapy in managing depression occurring during radiotherapy.</p>","PeriodicalId":11285,"journal":{"name":"Dose-Response","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10666705/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138458578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insecticidal and Repellent Activities of Four Essential Oils Against <i>Sitophilus zeamais</i> (Coleoptera: Curculionidae).","authors":"Hany Ahmed Fouad, Cláudio Augusto Gomes da Câmara, Marcílio Martins de Moraes, Wagner de Souza Tavares, Jesusa Crisostomo Legaspi, José Cola Zanuncio","doi":"10.1177/15593258231210263","DOIUrl":"https://doi.org/10.1177/15593258231210263","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to evaluate the efficacy of <i>Corymbia citriodora</i>, <i>Melaleuca alternifolia</i> (Myrtaceae), <i>Mentha</i> × <i>piperita</i> (Lamiaceae), and <i>Schinus terebinthifolius</i> (Anacardiaceae) essential oils as an alternative to manage <i>Sitophilus zeamais</i> (Coleoptera: Curculionidae) adults.</p><p><strong>Methods: </strong>Acute contact toxicity, acute toxicity on treated maize grain, fumigation toxicity, repellency bioassays, and GC-MS analysis of the essential oils were carried out.</p><p><strong>Results: </strong><i>Corymbia citriodora</i>, <i>M. alternifolia</i>, <i>M.</i> × <i>piperita</i>, and <i>S. terebinthifolius</i> oils were toxic at different levels to <i>S. zeamais</i> through residual contact, ingestion and via fumigation, and were also repellent to adults of this pest. <i>Melaleuca alternifolia</i> oil was the most active in contact (LC₅₀ = 18.98 μL.mL^-1), ingestion (LC₅₀ = 1.03 μL.g^-1), and fumigant (LC₅₀ = 20.05 μL.L^-1 air) bioassays. Citronelal (53.6% in <i>C. citriodora</i>), terpinen-4-ol (46.9% in <i>M. alternifolia</i>), menthol (44.8% in <i>M. × piperita</i>), and <i>β</i>-caryophyllene (16.2% in <i>S terebinthifolius</i>) are the major constituents of these oils.</p><p><strong>Conclusions: </strong><i>Melaleuca alternifolia</i> and <i>M. × piperita</i> essential oils can be used by residual contact, while those of <i>C. citriodora</i>, <i>M. alternifolia</i>, and <i>M. × piperita</i> by mixing with maize grains. <i>Melaleuca alternifolia</i> essential oil can be used as a fumigant, while those of <i>C. citriodora</i> and <i>S. terebinthifolius</i> as repellents for <i>S. zeamais</i> adults.</p>","PeriodicalId":11285,"journal":{"name":"Dose-Response","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10664452/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138458518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Complexing Properties of Synthesized 1,3,5-Triaza-7-Phosphaadamantane Derivatives Towards Some Lanthanides and Transition Metal Cations With Significant Antimicrobial and Antioxidant Activities.","authors":"Lassaad Baklouti, Besma Mellah, Waleed S Koko","doi":"10.1177/15593258231216274","DOIUrl":"https://doi.org/10.1177/15593258231216274","url":null,"abstract":"<p><p>The synthesis of new water-soluble N-alkylated derivatives of 1,3,5-triaza-7-phosphaadamantane is presented. Ru(PPh₃)₂Cl₂ has been used to react with 1-(4-nitrobenzyl)-3,5-triaza-1-azonia-7-phosphaadamantane bromide (PTAR). By using elemental analysis, NMR, and IR spectroscopy, the obtained compounds were identified. The UV-visible absorption spectroscopy has been used to monitor the complexation of various transition metal cations. Studies on conductivity have been utilized to validate the complexes' stoichiometries. Using the disc diffusion method, five bacteria strains were used for the study of the antimicrobial activity of compounds <b>1-3</b>. All tested pathogens, including <i>M luteus</i> LB 141107, were found to have strong biologic activity against the compounds tested in this study. Additionally, DPPH (2,2-diphenyl-1-picrylhydrazyl) has been tested for its ability to scavenge hydrogen peroxide and free radicals. According to our results, these compounds exhibit excellent radical scavenging properties.</p>","PeriodicalId":11285,"journal":{"name":"Dose-Response","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10655660/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138458577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green Lead Nanoparticles Induced Apoptosis and Cytotoxicity in MDA-MB-231 Cells by Inducing Reactive Oxygen Species and Caspase 3/7 Enzymes.","authors":"Wadyan Lafi Alsulami, Daoud Ali, Bader O Almutairi, Khadijah N Yaseen, Saad Alkahtani, Rafa A Almeer, Saud Alarifi","doi":"10.1177/15593258231214364","DOIUrl":"10.1177/15593258231214364","url":null,"abstract":"<p><p>Nanoparticles are widely used in the pharmaceutical, agriculture, and food processing industries. In this study, we have synthesized green lead nanoparticles (gPbNPs) by using an extract of <i>Ziziphus spina-christi</i> leaves and determined their cytotoxic and apoptotic effect on the human breast cancer MDA-MB-231 cell line. gPbNPs were characterized by using X-ray diffraction (XRD), energy dispersive X-ray (EDX) scanning electron microscope (SEM), and transmission electron microscope (TEM). The toxicity of gPbNPs was determined on the MDA-MB-231 cell line using MTT and NRU assays and as a result cell viability was reduced in a concentration-dependent manner. MDA-MB-231 cells were more sensitive at the highest concentration of gPbNPs exposure. In this experiment, we observed the production of intracellular ROS in cells, and induction of caspase 3/7 was higher in cells at 42 µg/ml of gPbNPs. Moreover, the Bax gene was upregulated and the Bcl-2 gene was downregulated and increased caspase 3/7 activity confirmed the apoptotic effect of gPbNPs in cells. Our observation showed that gPbNPs induced cell toxicity, increased generation of intracellular ROS, and gene expression of Bcl-2 and Bax in the MDA-MB-231 cell line. In conclusion, these findings demonstrated that gPbNPs executed toxic effects on the MDA-MB-231 cell line through activating caspase 3/7 activity.</p>","PeriodicalId":11285,"journal":{"name":"Dose-Response","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10638878/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89717345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Air Pollution and Cardiac Diseases: A Review of Experimental Studies.","authors":"Laura C Palacio, Diana C Pachajoa, Carlos A Echeverri-Londoño, Javier Saiz, Catalina Tobón","doi":"10.1177/15593258231212793","DOIUrl":"https://doi.org/10.1177/15593258231212793","url":null,"abstract":"<p><p>Air pollution is associated with around 6.5 million premature deaths annually, which are directly related to cardiovascular diseases, and the most dangerous atmospheric pollutants to health are as follows: NO₂, SO₂, CO, and PM. The mechanisms underlying the observed effects have not yet been clearly defined. This work aims to conduct a narrative review of experimental studies to provide a more comprehensive and multiperspective assessment of how the effect of atmospheric pollutants on cardiac activity can result in the development of cardiac diseases. For this purpose, a review was carried out in databases of experimental studies, excluding clinical trials, and epidemiological and simulation studies. After analyzing the available information, the existence of pathophysiological effects of the different pollutants on cardiac activity from exposure during both short-term and long-term is evident. This narrative review based on experimental studies is a basis for the development of recommendations for public health.</p>","PeriodicalId":11285,"journal":{"name":"Dose-Response","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10625734/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71479329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Curcumin's Dose-Dependent Attenuation of Gastric Cancer Cell Progression Via the PI3K Pathway Blockade.","authors":"Gen Xi, Qingtao Dong, Bo Yang, Desheng Jiao, Shahanavaj Khan","doi":"10.1177/15593258231203585","DOIUrl":"https://doi.org/10.1177/15593258231203585","url":null,"abstract":"<p><p><b>Background:</b> Gastric cancer stands as a primary cause of cancer-related deaths worldwide, making the discovery of new therapeutic agents essential for enhancing treatment outcomes. Curcumin, a polyphenolic compound found in turmeric (<i>Curcuma longa</i>), has demonstrated potential in multiple cancer types due to its anti-cancer characteristics. This research aimed to examine the impact of curcumin on gastric cancer cell growth, migration, and invasion, as well as its influence on the phosphoinositide 3-kinase (PI3K) signaling cascade. <b>Methods:</b> Gastric cancer cell lines were exposed to varying curcumin concentrations, followed by assessments of cell viability, migration, and invasion. Furthermore, gene and protein expression levels associated with the PI3K signaling cascade were evaluated using reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis. <b>Results:</b> The findings revealed a dose-dependent decrease in cell viability, migration, and invasion in gastric cancer cells treated with curcumin. Additionally, curcumin administration led to the downregulation of key genes and proteins within the PI3K signaling process, such as PI3K, Akt, and mTOR. <b>Conclusion:</b> These findings propose that curcumin may exercise its anti-cancer effects on gastric cancer cells, partly by suppressing the PI3K signaling pathway. This study's outcomes support curcumin's potential as a therapeutic agent for gastric cancer and encourage further exploration of its underlying molecular mechanisms and in vivo effectiveness.</p>","PeriodicalId":11285,"journal":{"name":"Dose-Response","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10625731/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71479330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing the Risk of Secondary Cancer Induction in Radiosensitive Organs During Trigeminal Neuralgia Treatment With Gamma Knife Radiosurgery: Impact of Extracranial Dose.","authors":"Ghazale Geraily,&nbsp;Ali Ameri,&nbsp;Atefeh Mahmoudi,&nbsp;Mohadese Moafee,&nbsp;Javad Teymouri","doi":"10.1177/15593258231210432","DOIUrl":"https://doi.org/10.1177/15593258231210432","url":null,"abstract":"<p><strong>Purpose: </strong>Gamma knife radiosurgery (GKRS) delivers high-dose external radiation to a small intracranial lesion. However, scattering and leaked radiation can deposit a portion of the dose outside the radiation field, which may pose a risk to radiation-sensitive patients, such as pregnant women. Trigeminal Neuralgia (TN) is treated with one of the highest GKRS doses (80-90 Gy). This study aimed to estimate the risk of secondary cancer induction in the uterus, ovaries, thyroid gland, and eyes of TN patients undergoing GKRS.</p><p><strong>Methods: </strong>Radiation doses to the uterus, ovary, eyes, and thyroid gland were measured for 25 female TN patients, with a mean age of 35 years, utilizing Thermo Luminescent Dosimeters (TLD).</p><p><strong>Results: </strong>The mean absorbed dose for the uterus, ovary, thyroid gland, and eyes were .63 ± .24, .471 ± .2, 8.26 ± 1.01, and 10.64 ± 1.08 cGy, respectively. Lifetime Attributable Risk (LAR) has been calculated using BEIR VII (2006) method. LAR for the uterus, ovary, and thyroid gland was 1, 2, and 23, respectively.</p><p><strong>Conclusion: </strong>The results of this study and its comparison with standard values demonstrate that on average, mean doses to mentioned organs were smaller than their tolerance doses, and there is no limitation to treating patients suffering from TN by GK.</p>","PeriodicalId":11285,"journal":{"name":"Dose-Response","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10605703/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71411132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}