Developmental Dynamics最新文献

{"title":"EphB2, EphB4, and ephrin-B1 expression and localization in postnatal developing epididymis in mice.","authors":"Md Royhan Gofur, Kazushige Ogawa","doi":"10.1002/dvdy.752","DOIUrl":"https://doi.org/10.1002/dvdy.752","url":null,"abstract":"<p><strong>Background: </strong>Eph receptors and ephrin ligands, the transmembrane proteins, function as a mechanism of communication between cells. Therefore, we intended to explore the expression array of EphB2 and EphB4 receptors and ephrin-B1 ligand in postnatal developing mouse epididymis during 1 day to 8 weeks using RT-PCR amplification and immunofluorescence staining.</p><p><strong>Results: </strong>RT-PCR analysis indicated that the expression levels of EphB2, EphB4, and ephrin-B1 in the epididymis declined with the advancement of age during the initial phases of postnatal development and stayed relatively near to adult levels until 4 weeks. We discovered that the predominant compartments expressing EphB2/B4 and ephrin-B1 emerged in the excurrent duct epithelia of postnatal developing epididymis until 3 weeks. Consequently, even before spermatozoa reach the excurrent duct in epididymis, at the age of 3 weeks, the epididymal excurrent duct system exhibits characteristics similar to those of an adult in terms of expression of EphB2/B4 and ephrin-B1. Moreover, ephrin-B1 was expressed in epididymal epithelial cells throughout the development and EphB4 was expressed only in early postnatal stages while basal cells expressed EphB4 throughout the postnatal development.</p><p><strong>Conclusion: </strong>The study represents the first expression analysis of ephrin-B1, EphB2, and EphB4 in the normal mouse epididymis during the postnatal development.</p>","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142399686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elp1 function in placode-derived neurons is critical for proper trigeminal ganglion development.","authors":"Margaret A Hines, Lisa A Taneyhill","doi":"10.1002/dvdy.749","DOIUrl":"10.1002/dvdy.749","url":null,"abstract":"<p><strong>Background: </strong>The trigeminal nerve is the largest cranial nerve and functions in somatosensation. Cell bodies of this nerve are positioned in the trigeminal ganglion, which arises from the coalescence of neural crest and placode cells. While this dual cellular origin has been known for decades, the molecular mechanisms controlling trigeminal ganglion development remain obscure. We performed RNA sequencing on the forming chick trigeminal ganglion and identified Elongator acetyltransferase complex subunit 1 (Elp1) for further study. Mutations in ELP1 cause familial dysautonomia (FD), a fatal disorder characterized by the presence of smaller trigeminal nerves and sensory deficits. While Elp1 has established roles in neurogenesis, its function in placode cells during trigeminal gangliogenesis has not been investigated.</p><p><strong>Results: </strong>To this end, we used morpholinos to deplete Elp1 from chick trigeminal placode cells. Elp1 knockdown decreased trigeminal ganglion size and led to aberrant innervation of the eye by placode-derived neurons. Trigeminal nerve branches also appeared to exhibit reduced axon outgrowth to target tissues.</p><p><strong>Conclusions: </strong>These findings reveal a new role for Elp1 in placode-derived neurons during chick trigeminal ganglion development. These results have potential high significance to provide new insights into trigeminal ganglion development and the etiology of FD.</p>","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11978919/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142388833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A network of transient domains for breaking symmetry during anterior-posterior axis formation in the porcine embryo.","authors":"Ruben Plöger, Nikoloz Tsikolia, Christoph Viebahn","doi":"10.1002/dvdy.739","DOIUrl":"https://doi.org/10.1002/dvdy.739","url":null,"abstract":"<p><p>Breaking radial symmetry for anterior-posterior axis formation is one of the key developmental steps of vertebrate gastrulation and is established through a succession of transient domains defined by morphology or gene expression. Three such domains were interpreted recently in the rabbit to be part of a \"three-anchor-point model\" for axis formation. To answer the question as to whether the model is generally applicable to mammals, the dynamic expression patterns of four marker genes were analyzed in the pig, where gastrulating epiblast forms from half the inner cell mass: EOMES and PKDCC transcripts display decreasing expression intensities in the anterior hypoblast and-together with WNT3-increasing intensity in the anterior streak domain and the node; TBX6 expression changes from an initial central expression to exclusive expression in the posterior extremity of the primitive streak. The anterior streak domain has thus a molecular footprint similar to the one in the rabbit, the end node shares TBX6 between the species, while the anterior hypoblast-mirroring specific porcine epiblast derivation and fate-is marked by PKDCC instead of WNT3. The molecular similarities in transient domains point to conserved mechanisms for establishing the mammalian anterior-posterior axis and, possibly, breaking radial symmetry.</p>","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142388832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional significance of earthworm clitellum in regulating the various biological aspects of cell survival and regeneration","authors":"Jackson Durairaj Selvan Christyraj,&nbsp;Ashwin Barath Vaidhyalingham,&nbsp;Chandini Sengupta,&nbsp;Kamarajan Rajagopalan,&nbsp;Kayalvizhi Vadivelu,&nbsp;Nandha Kumar Suresh,&nbsp;Bharathi Venkatachalam","doi":"10.1002/dvdy.751","DOIUrl":"10.1002/dvdy.751","url":null,"abstract":"<p>Earthworms are a highly abundant species in nature, with nearly 7000 different species being discovered. Despite the similarities in morphology among earthworm species, their regeneration capabilities vary based on the clitellum. The clitellum plays a crucial role in the clitellum-dependent worms, as it is involved in the processes of regeneration and reproduction in earthworms. The fascinating characteristic of the clitellum, which serves as a hub for stem cells in clitellum-dependent worms, plays a crucial role in various biological processes that require further exploration. This review focuses on the overall physiological functions and uncovers the lesser-known roles of the clitellum that have been documented in various research articles. In recent times, numerous studies have been conducted using the earthworm model to explore various areas. In that regard, the clitellum's different roles in regulating and controlling stem cells, the regeneration process, regulation of organogenesis, stress response, aging, autotomy, and various features have been briefly discussed. Ultimately, we emphasized the unique and versatile role of the clitellum in the animal model, making it an ideal choice for studying development, regeneration, stem cells, organogenesis, toxicology, autotomy, and aging response.</p>","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":"254 3","pages":"212-221"},"PeriodicalIF":2.0,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/dvdy.751","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142380261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"foxe1 mutant zebrafish show indications of a hypothyroid phenotype and increased sensitivity to ethanol for craniofacial malformations","authors":"Sophie T. Raterman,&nbsp;Frank A. D. T. G. Wagener,&nbsp;Jan Zethof,&nbsp;Vincent Cuijpers,&nbsp;Peter H. M. Klaren,&nbsp;Juriaan R. Metz,&nbsp;Johannes W. Von den Hoff","doi":"10.1002/dvdy.745","DOIUrl":"10.1002/dvdy.745","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>FOXE1 mutations in humans are associated with cleft palate and hypothyroidism. We previously developed a <i>foxe1</i> mutant zebrafish demonstrating mineralization defects in larvae. In the present study, we investigate the thyroid status and skeletal phenotype of adult <i>foxe1</i> mutants.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Mutant fish have increased expression of <i>tshβ</i> in the pituitary, and of hepatic <i>dio1</i> and <i>dio2</i>. In plasma, we found higher Mg levels. Together these findings are indicative of hypothyroidism. We further observed mineralization defects in scales due to enhanced osteoclast activity as measured by increased expression levels of <i>tracp</i>, <i>ctsk</i>, and <i>rankl</i>. Gene–environment interactions in the etiology of FOXE1-related craniofacial abnormalities remain elusive, which prompts the need for models to investigate genotype–phenotype associations. We here investigated whether ethanol exposure increases the risk of developing craniofacial malformations in <i>foxe1</i> mutant larvae that we compared to wild types. We found in ethanol-exposed mutants an increased incidence of developmental malformations and marked changes in gene expression patterns of cartilage markers (<i>sox9a</i>), apoptotic markers (<i>casp3b</i>), retinoic acid metabolism (<i>cyp26c1</i>), and tissue hypoxia markers (<i>hifaa, hifab</i>).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Taken together, this study shows that the <i>foxe1</i> mutant zebrafish recapitulates phenotypes associated with FOXE1 mutations in human patients and a clear <i>foxe1</i>-ethanol interaction.</p>\u0000 </section>\u0000 </div>","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":"254 3","pages":"240-256"},"PeriodicalIF":2.0,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/dvdy.745","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142364776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seasonal heterochrony of reproductive development and gene expression in a polymorphic salamander","authors":"Madison A. Herrboldt,&nbsp;Claire N. C. Wright,&nbsp;Ronald M. Bonett","doi":"10.1002/dvdy.744","DOIUrl":"10.1002/dvdy.744","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Life cycle evolution includes ecological transitions and shifts in the timing of somatic and reproductive development (heterochrony). However, heterochronic changes can be tissue-specific, ultimately leading to the differential diversification of traits. Salamanders exhibit alternative life cycle polymorphisms involving either an aquatic to terrestrial metamorphosis (biphasic) or retention of aquatic larval traits into adulthood (paedomorphic). In this study, we used gene expression and histology to evaluate how life cycle evolution impacts temporal reproductive patterns in males of a polymorphic salamander.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>We found that heterochrony shifts the distribution of androgen signaling in the integument, which is correlated with significant differences in seasonal reproductive gland development and pheromone gene expression. In the testes, androgen receptor (<i>ar</i>) expression does not significantly vary between morphs or across seasons. We found significant differences in the onset of spermatogenesis, but by peak breeding season the testes were the same with respect to both histology and gene expression.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>This study provides an example of how seasonal heterochronic shifts in tissue-specific <i>ar</i> gene expression can disparately impact seasonal development and expression patterns across tissues, providing a potential mechanism for differential diversification of reproductive traits.</p>\u0000 </section>\u0000 </div>","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":"254 4","pages":"330-347"},"PeriodicalIF":2.0,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142364778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hypoxia regulate developmental coronary angiogenesis potentially through VEGF-R2- and SOX17-mediated signaling","authors":"Halie E. Vitali,&nbsp;Bryce Kuschel,&nbsp;Chhiring Sherpa,&nbsp;Brendan W. Jones,&nbsp;Nisha Jacob,&nbsp;Syeda A. Madiha,&nbsp;Sam Elliott,&nbsp;Eddie Dziennik,&nbsp;Lily Kreun,&nbsp;Cora Conatser,&nbsp;Bhupal P. Bhetwal,&nbsp;Bikram Sharma","doi":"10.1002/dvdy.750","DOIUrl":"10.1002/dvdy.750","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>The development of coronary vessels in embryonic mouse heart involves various progenitor populations, including sinus venosus (SV), endocardium, and proepicardium. ELA/APJ signaling is known to regulate coronary growth from the SV, whereas VEGF-A/VEGF-R2 signaling controls growth from the endocardium. Previous studies suggest hypoxia might regulate coronary growth, but its specific downstream pathways are unclear. In this study, we further investigated the role of hypoxia and have identified SOX17- and VEGF-R2-mediated signaling as the potential downstream pathways in its regulation of developmental coronary angiogenesis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>HIF-1α stabilization by knocking out von Hippel Lindau (VHL) protein in the myocardium (cKO) disrupted normal coronary angiogenesis in embryonic mouse hearts, resembling patterns of accelerated coronary growth. VEGF-R2 expression was increased in coronary endothelial cells under hypoxia in vitro and in VHL cKO hearts in vivo. Similarly, SOX17 expression was increased in the VHL cKO hearts, while its knockout in the endocardium disrupted normal coronary growth.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>These findings provide further evidence that hypoxia regulates developmental coronary growth potentially through VEGF-R2 and SOX17 pathways, shedding light on mechanisms of coronary vessel development.</p>\u0000 </section>\u0000 </div>","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":"254 2","pages":"174-188"},"PeriodicalIF":2.0,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142364777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of Meis2 knockout mice reveals Sonic hedgehog-mediated patterning of the cochlear duct","authors":"Hei Yeun Koo,&nbsp;Jae Hwan Oh,&nbsp;María Beatriz Durán Alonso,&nbsp;Iris López Hernández,&nbsp;Margarita González-Vallinas,&nbsp;María Teresa Alonso,&nbsp;Juan J. Tena,&nbsp;Alejandro Gil-Gálvez,&nbsp;Fernando Giraldez,&nbsp;Jinwoong Bok,&nbsp;Thomas Schimmang","doi":"10.1002/dvdy.747","DOIUrl":"10.1002/dvdy.747","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>The mechanisms underlying the formation of complex structures such as during the outgrowth of the cochlear duct are still poorly understood.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>We have analyzed the morphological and molecular changes associated with cochlear development in mouse mutants for the transcription factor Meis2, which show defective coiling of the cochlea. These morphological abnormalities were accompanied by the formation of ectopic and extra rows of sensory hair cells. Gene profiling of otic vesicles from Meis2 mutants revealed a dysregulation of genes that are potentially involved in Sonic hedgehog (Shh)-mediated patterning of the cochlear duct. Like in Shh mutants, Meis2 defective mice showed a loss of genes that are expressed in the apical part of the cochlear duct.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Taken together, these data reveal that the loss of Meis2 leads to a phenotype that resembles Shh mutants, suggesting that Meis2 is instrumental for cochlear Shh signaling. The modulation of the same subset of genes provides an interesting insight into which Shh responsive genes are essential for outgrowth and patterning of the cochlear duct.</p>\u0000 </section>\u0000 </div>","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":"254 4","pages":"365-372"},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142343560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial highlights","authors":"Paul A. Trainor","doi":"10.1002/dvdy.748","DOIUrl":"10.1002/dvdy.748","url":null,"abstract":"&lt;p&gt;Every organism is a model organism for understanding development, evolution, disease, and regeneration, and we have only begun to scratch the surface of the interdisciplinary genetic, molecular, cellular, and developmental mechanisms that regulate these biological processes. These “Highlights” denote exciting advances recently reported in &lt;i&gt;Developmental Dynamics&lt;/i&gt; that illustrate the complex dynamics of developmental biology.&lt;/p&gt;&lt;p&gt;&lt;b&gt;Blood Development&lt;/b&gt; “Establishment of a Diamond-Blackfan anemia like (DBAL) model in zebrafish”, by Yiming Ling, Jiaye Wu, Yushi Liu, Panpan Meng, Ying Sun, Dejian Zhao, and Qing Lin; &lt;i&gt;Dev Dyn&lt;/i&gt; 253:10, pp. 906–921. https://doi.org/10.1002/dvdy.703. Red blood cells (erythrocytes), which have a typical lifespan of 90–120 days, are essential for oxygen delivery throughout the body. Deficiencies in erythrocyte number or morphology, or hemoglobin levels can result in anemia. Zebrafish, which have transparent embryos, are a powerful model for studying human hematological disorders. In this study, the authors generated &lt;i&gt;epoa&lt;/i&gt;-deficient zebrafish as a model of Diamond–Blackfan anemia like (DBAL), which occurs in humans in association with recessive loss-of-function mutations in EPO. EPO is crucial for erythrocyte development and oxygen transport and &lt;i&gt;epoa&lt;/i&gt;&lt;sup&gt;&lt;i&gt;szy8/zy8&lt;/i&gt;&lt;/sup&gt; mutants carrying the human EPO mutation c.530G&gt;A, developed DBAL due to reduced &lt;i&gt;EPO&lt;/i&gt; expression. The severe anemia observed in &lt;i&gt;epoa&lt;/i&gt;&lt;sup&gt;&lt;i&gt;szy8/zy8&lt;/i&gt;&lt;/sup&gt; mutant zebrafish can be used to screen drugs for treating epoa-deficiency anemia, and recombinant human EPO significantly improved erythrocyte numbers. Zebrafish &lt;i&gt;epoa&lt;/i&gt; models of DBAL are therefore beneficial for in vivo assessments of patient-derived &lt;i&gt;EPO&lt;/i&gt; variants, and for developing potential therapeutic approaches for treating DBAL.&lt;/p&gt;&lt;p&gt;&lt;b&gt;Craniofacial and Hair Development&lt;/b&gt; “Lineage-specific requirements of Alx4 function in craniofacial and hair development” by Yu Lan, Zhaoming Wu, Han Liu, and Rulang Jiang; &lt;i&gt;Dev Dyn&lt;/i&gt; 253:10, pp. 940–948. https://doi.org/10.1002/dvdy.705. The ALX family of transcription factors are key regulators of craniofacial development. Variants in &lt;i&gt;ALX4&lt;/i&gt; have been associated with autosomal dominant parietal foramina and autosomal recessive frontonasal dysplasia with alopecia in humans, but the mechanisms connecting their etiology and pathogenesis remain poorly understood. &lt;i&gt;Alx4&lt;/i&gt; is broadly expressed throughout development, making it difficult to determine its cell-autonomous and non-cell autonomous functions. Here the authors report the generation and characterization of &lt;i&gt;Alx4&lt;/i&gt;&lt;sup&gt;&lt;i&gt;fx/fx&lt;/i&gt;&lt;/sup&gt; conditional mice as a valuable new resource for investigating the pathogenic mechanisms underlying ALX4-related developmental disorders and alopecia. &lt;i&gt;Alx4&lt;/i&gt; tissue-specific loss-of-function in neural crest cells and limb bud mesenchyme, results in craniofacial and limb bud developmental def","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":"253 10","pages":"880-881"},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/dvdy.748","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142343565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Patterns of early embryogenesis and growth in the olfactory system of chick (Gallus gallus domesticus) based on iodine-enhanced micro-computed tomography","authors":"Aneila V. C. Hogan,&nbsp;Donald G. Cerio,&nbsp;Gabriel S. Bever","doi":"10.1002/dvdy.746","DOIUrl":"10.1002/dvdy.746","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>The vertebrate olfactory system entails a complex set of neural/support structures that bridge morphogenetic regions. The developmental mechanisms coordinating this bridge remain unclear, even for model organisms such as chick, <i>Gallus gallus</i>. Here, we combine previous growth data on the chick olfactory apparatus with new samples targeting its early embryogenesis. The purpose is to illuminate how early developmental dynamics integrate with scaling relationships to produce adult form and, potentially, evolutionary patterns. Olfactory structures, including epithelium, turbinate, nerve, and olfactory bulb, are considered in the context of neighboring nasal and brain structures.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Axonal outgrowth from the olfactory epithelium, which eventually connects receptor neurons with the brain, begins earlier than previously established. This dynamic marks the beginning of a complex pattern of early differential growth wherein the olfactory bulbs scale with positive allometry relative to both brain volume and turbinate area, which in turn scale isometrically with one another.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The mechanisms driving observed patterns of organogenesis and growth remain unclear awaiting experimental evidence. We discuss competing hypotheses, including the possibility that broad-based isometry of olfactory components reflects constraints imposed by high levels of functional/structural integration. Such integration would include the frontonasal prominence having a strong influence on telencephalic patterning.</p>\u0000 </section>\u0000 </div>","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":"254 4","pages":"348-364"},"PeriodicalIF":2.0,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142343563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}