Developmental Dynamics最新文献

{"title":"The endocannabinoid system regulates both ependymoglial and neuronal cell responses to a tail amputation in the axolotl.","authors":"Michael Tolentino, Sarah E Walker, Gaynor E Spencer, Robert Carlone","doi":"10.1002/dvdy.70035","DOIUrl":"https://doi.org/10.1002/dvdy.70035","url":null,"abstract":"<p><strong>Background: </strong>The endocannabinoid system is a neuromodulatory system implicated in cellular processes during both development and regeneration. The Mexican axolotl, one of only a few vertebrates capable of central nervous system regeneration, was used to examine the role of the endocannabinoid system in the regeneration of the tail and spinal cord following amputation.</p><p><strong>Results: </strong>The endocannabinoid receptor CB1 was upregulated in the regenerating axolotl spinal cord by 4 hours following tail amputation, and this upregulation persisted for at least 14 days. The endocannabinoid receptor CB2 was upregulated later, between 7 and 14 days after tail amputation. Both CB1 and CB2 were located in ependymoglia and neurons within the regenerating spinal cord. Treatment with inverse agonists to inhibit CB1 (AM251) or CB2 (AM630) inhibited spinal cord and tail regeneration. During the first 7 days after injury, CB1 and CB2 expression was also necessary for the proliferation of ependymoglial cells and the regeneration of axons into the newly regenerated tail tissue. However, only CB1 was necessary for the differentiation of ependymoglia into immature neurons.</p><p><strong>Conclusions: </strong>These studies are the first to examine the role of the endocannabinoid system during spinal cord regeneration in a regeneration-competent vertebrate.</p>","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144076812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in culturing of the sea star Patiria miniata.","authors":"Vanessa Barone, Luisa Coronado, Deka Ismail, Sareen Fiaz, Deirdre C Lyons","doi":"10.1002/dvdy.70040","DOIUrl":"https://doi.org/10.1002/dvdy.70040","url":null,"abstract":"<p><strong>Background: </strong>The use of the sea star Patiria miniata as a model system has produced groundbreaking advances in a disparate set of biomedical research fields, including embryology, immunology, regeneration, cell biology, and evolution of development. Nonetheless, the life cycle of P. miniata has not yet been closed in the laboratory, precluding the generation of stable transgenic and mutant lines, which would greatly expand the toolset for experimentation with this model system. Rearing P. miniata in the laboratory has been challenging due to limited knowledge about metamorphosis cues, feeding habits of juveniles, and their relatively long generation time.</p><p><strong>Results: </strong>Here we report protocols to rear P. miniata embryos through sexual maturity in a laboratory setting. We provide detailed staging of early embryonic development at different temperatures, and show that larvae can be raised to competence in as little as 15 days. We find that retinoic acid induces metamorphosis effectively and present methods to rear juveniles on commercially available foods. We show that in a flow-through system, juveniles double in size every 2 months and reach sexual maturity in approximately 2 years.</p><p><strong>Conclusions: </strong>We report the first example of P. miniata raised through sexual maturity in a laboratory setting, paving the way for the generation of stable mutant sea star lines.</p>","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144076743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic potential of stem cells in colorectal cancer management: Current trends and future prospects.","authors":"Manya D Desai, Namrata B Parmar, Isha T Shah, Priyajeet S Parekh, Rajanikant Patel, Mehul R Chorawala","doi":"10.1002/dvdy.70042","DOIUrl":"https://doi.org/10.1002/dvdy.70042","url":null,"abstract":"<p><p>Colorectal cancer (CRC) ranks among the leading causes of cancer-related morbidity and mortality worldwide. Despite progress in understanding its molecular intricacies, the management of CRC, especially in advanced stages, remains a significant clinical hurdle. This review delves into the evolving landscape of stem cell-based therapeutic strategies in CRC, with a specific focus on the interplay between cancer stem cells (CSCs) and CRC pathogenesis and treatment resistance. Highlighting the pivotal roles of CSCs in tumor initiation, progression, metastasis, and recurrence, the review comprehensively examines their involvement in CRC, ranging from normal colonic tissue to cancer initiation. The potential of stem cells for medicinal purposes in CRC management is explored, encompassing diverse modalities such as transplantation, differentiation therapy, immunotherapy, and gene/cell-based approaches. Challenges and opportunities associated with these strategies are also evaluated, providing insights into their clinical potential and limitations. The review also appraises preclinical investigations contributing to the understanding of CRC and stem cells. Current clinical trials, patient stratification strategies, and regulatory considerations related to stem cell-based therapies in CRC are scrutinized. Furthermore, the review explores emerging trends and future directions, including developments in stem cell technologies and ethical considerations. It highlights the transformative potential of stem cell-based therapeutic strategies in CRC.</p>","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143997103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamics of postnatal bone development and epiphyseal synostosis in the caprine autopod.","authors":"Christopher J Panebianco, Maha Essaidi, Elijah Barnes, Ashley Williams, Karin Vancíková, Margot C Labberté, Pieter Brama, Niamh C Nowlan, Joel D Boerckel","doi":"10.1002/dvdy.70038","DOIUrl":"https://doi.org/10.1002/dvdy.70038","url":null,"abstract":"<p><strong>Background: </strong>Bones develop to structurally balance strength and mobility. Bone developmental dynamics are influenced by whether an animal is ambulatory at birth. Precocial species, which are ambulatory at birth, develop advanced skeletal maturity in utero and experience postnatal development under mechanical loading. Here, we characterized postnatal bone development in the lower forelimbs of precocial goats using microcomputed tomography and histology. Our analysis focused on the two phalanges 1 (P1) bones and the partially fused metacarpal bone of the goat autopod from birth through adulthood.</p><p><strong>Results: </strong>P1 cortical bone densified rapidly after birth, but cortical thickness increased continually through adulthood. Upon normalization by body mass, the P1 normalized polar moment of inertia was constant over time, suggestive of changes correlating with ambulatory loading. P1 trabecular bone increased in trabecular number and thickness until sexual maturity (12 months), while metacarpal trabeculae grew primarily through trabecular thickening. Unlike prenatal synostosis (i.e., bone fusion) of the metacarpal diaphysis, synostosis of the epiphyses occurred postnatally, prior to growth plate closure, through a unique fibrocartilaginous endochondral ossification.</p><p><strong>Conclusions: </strong>These findings implicate ambulatory loading in postnatal bone development of precocial goats and identify a novel postnatal synostosis event in the caprine metacarpal epiphysis.</p>","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143987352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rodent monocyte-derived macrophages do not express CD163: Comparative analysis using macrophages from living boreoeutherians.","authors":"Yoichi Saito, Yukio Fujiwara, Yasuka L Yamaguchi, Satomi S Tanaka, Kyoko Miura, Yoshiyuki Hizukuri, Kyoko Yamashiro, Yasuhiro Hayashi, Yuta Nakashima, Yoshihiro Komohara","doi":"10.1002/dvdy.70036","DOIUrl":"https://doi.org/10.1002/dvdy.70036","url":null,"abstract":"<p><strong>Background: </strong>CD163 is a scavenger receptor predominantly expressed on the surfaces of macrophages in various mammalian species and is a marker of anti-inflammatory (M2-like) macrophages. High density of CD163-positive tumor-associated macrophages (TAMs) is associated with worse prognosis in various patient tumors. Interestingly, studies on mice have shown that CD163-positive TAMs only infiltrate the margins of tumor tissues, not the center. Based on these observations, we hypothesized that circulating monocyte-derived macrophages (MDMs), which are the origin of most TAMs, do not express CD163 in mice.</p><p><strong>Results: </strong>We examined CD163 expression in MDMs, differentiated from healthy animals in vitro, and in normal, pathogenic, and tumorigenic macrophages infiltrating various tumors and organs across multiple species including primates, rodents, cetartiodactylans, and carnivores. We found that MDMs, including TAMs, do not express CD163 in mice. Our findings also suggest that murine CD163-positive macrophages likely originate from a specific subset of resident macrophages, namely fetal liver monocytes/macrophages, as indicated by fetal analysis. Furthermore, we revealed that the CD163-negative expression pattern in MDMs is a trait shared by the rodent clade.</p><p><strong>Conclusions: </strong>Rodent MDMs do not express CD163, a phenotype not shared with MDMs of other mammals. Our findings caution against the extrapolation of rodent experimental results to other animal models.</p>","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143985069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial highlights","authors":"Paul A. Trainor","doi":"10.1002/dvdy.70030","DOIUrl":"https://doi.org/10.1002/dvdy.70030","url":null,"abstract":"&lt;p&gt;Every organism is a model organism for understanding development, evolution, disease, and regeneration, and we have only begun to scratch the surface of the interdisciplinary genetic, molecular, cellular, and developmental mechanisms that regulate these biological processes. These “Highlights” denote exciting advances recently reported in &lt;i&gt;Developmental Dynamics&lt;/i&gt; that illustrate the complex dynamics of developmental biology.&lt;/p&gt;&lt;p&gt;“Stage-by-stage exploration of normal embryonic development in the Arabian killifish, &lt;i&gt;Aphanius dispar&lt;/i&gt;” by Amena Alsakran, Rashid Minhas, Atyaf Hamied, Rod Wilson, Mark Ramsdale, and Tetsuhiro Kudoh Fan, &lt;i&gt;DevDyn&lt;/i&gt; 254.5, pp. 380–395, https://doi.org/10.1002/dvdy.738.&lt;/p&gt;&lt;p&gt;Teleost fish such as zebrafish and medaka are attractive models for studying developmental processes due to their small size and transparency. In addition, zebrafish and medaka have classically been used to model genetic diseases and infection. However, it is still desirable to develop new fish models that can facilitate the investigation of environmental changes and pollution in estuarine, marine, and high-salinity environments. The Arabian killifish (&lt;i&gt;Aphanius dispar&lt;/i&gt;) is a small teleost fish in which the embryo and chorion are very transparent, which facilitates high resolution and high throughput imaging. &lt;i&gt;A. dispar&lt;/i&gt; has been used as an effective biological control agent for mosquito larvae. This study investigated the step-by-step development of &lt;i&gt;A. dispar&lt;/i&gt; embryos, delineating key developmental milestones from the maternal to hatching stages, and demonstrated that temperature has a significant effect on embryonic development, with accelerated development at higher temperatures. &lt;i&gt;A. dispar&lt;/i&gt; exhibits broad thermal tolerance and extended independent feeding capabilities, making it a promising model organism for ecotoxicology and disease pathogenesis studies.&lt;/p&gt;&lt;p&gt;“Effects of life history strategies and habitats on limb regeneration in plethodontid salamanders” by Vivien Bothe, Hendrik Müller, Neil Shubin, and Nadia Fröbisch Fan, &lt;i&gt;DevDyn&lt;/i&gt; 254.5, pp. 396–419, https://doi.org/10.1002/dvdy.742.&lt;/p&gt;&lt;p&gt;Salamanders are renowned as the only tetrapod capable of fully regenerating its limbs. Indeed, in addition to their limbs and tails, salamanders are the only tetrapod able to regenerate various other anatomical structures, including organs such as the lens, heart, and liver, repeatedly and throughout their entire life. However, salamanders are a highly diverse clade of tetrapods with around 850 currently known species, and yet research on tetrapod regeneration has largely been based on a small number of salamander species, primarily the Mexican axolotl &lt;i&gt;Ambystoma mexicanum&lt;/i&gt; and, to a lesser extent, &lt;i&gt;Pleurodeles waltl&lt;/i&gt; and &lt;i&gt;Notophthalmus viridescens&lt;/i&gt;. Interestingly, some studies have suggested that certain salamanders may not be able to regenerate their limbs at all. Therefore, it remains to be dete","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":"254 5","pages":"378-379"},"PeriodicalIF":2.0,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/dvdy.70030","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143900906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tissue-specific requirement of Polr1D in the prothoracic gland for ecdysone-mediated developmental transitions in Drosophila melanogaster.","authors":"Bridget M Walker, Ryan J Palumbo, Bruce A Knutson","doi":"10.1002/dvdy.70029","DOIUrl":"https://doi.org/10.1002/dvdy.70029","url":null,"abstract":"<p><strong>Background: </strong>POLR1D is a shared subunit of RNA Polymerases I and III, which transcribe the rRNA incorporated into ribosomes. Mutations in POLR1D cause Treacher Collins syndrome, a craniofacial disorder that arises from impaired ribosome biogenesis in neural crest cells. Previously, we found that RNAi knockdown of Polr1D in several non-neural Drosophila tissues caused developmental defects that phenocopy mutations affecting ecdysone signaling. Ecdysone is a steroid hormone produced in the prothoracic gland (PG) of insects that triggers developmental transitions. Here, we show that Polr1D is required for PG development and ecdysone production to facilitate larval developmental transitions.</p><p><strong>Results: </strong>We found that Polr1D RNAi in the PG causes larval developmental arrest due to defective peripheral ecdysone signaling. We also found that Polr1D is required for the growth of PG cells and for maintaining nucleolar structure. We found that Polr1D is required for the synthesis of mature ribosomes and the production of the Pol III-transcribed 7SK RNA. Furthermore, developmental arrest of Polr1D RNAi larvae and Polr1D mutant (G30R) larvae was partially rescued by treatment with exogenous ecdysone.</p><p><strong>Conclusion: </strong>These results demonstrate a role for Drosophila Polr1D in PG development and suggest that disruptions in human Polr1D might impact additional cell types during development.</p>","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143995696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Myomaker and Myomixer are required for craniofacial myoblast fusion in zebrafish.","authors":"Zhanxiong Zhang, Pengzheng Yong, Qiaomu Hu, Shaojun Du","doi":"10.1002/dvdy.70031","DOIUrl":"https://doi.org/10.1002/dvdy.70031","url":null,"abstract":"<p><strong>Background: </strong>Craniofacial and trunk skeletal muscles are derived from different progenitor populations during development. Trunk skeletal muscles contain mostly multinucleated myofibers that are formed through myoblast fusion. However, myoblast fusion in craniofacial muscles and its molecular regulation are not well understood. Recent studies revealed that genetic mutations in MYOMAKER and MYOMIXER fusogens in humans cause Carey-Fineman-Ziter Syndrome (CFZS), characterized by facial weakness and lower jaw deformity.</p><p><strong>Results: </strong>Previous studies in zebrafish revealed that knockout of myomaker and myomixer resulted in deformed craniofacial formation. To establish the causal connection between loss of fusogen function and craniofacial deformities, we characterized myoblast fusion in zebrafish craniofacial muscles. Our results demonstrate that myomaker and myomixer are expressed in both slow and fast craniofacial muscles, and loss of these fusogens results in defects in craniofacial myoblast fusion. Interestingly, unlike trunk muscles of early embryos and larvae that show fast-fiber-specific fusogen expression and fusion while slow muscle fusion only occurs at 3 weeks post-fertilization, both slow and fast craniofacial muscles fuse as early as 3 days post-fertilization.</p><p><strong>Conclusions: </strong>Collectively, this study demonstrates that myomaker and myomixer are expressed in both slow and fast-twitch craniofacial muscles and are essential for myoblast fusion and the development of craniofacial muscles.</p>","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143988809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pre-oviposition development of the brown anole (Anolis sagrei).","authors":"Antonia Weberling, Natalia A Shylo, Bonnie K Kircher, Hannah Wilson, Melainia McClain, Marta Marchini, Katherine B Starr, Thomas J Sanger, Florian Hollfelder, Paul A Trainor","doi":"10.1002/dvdy.70027","DOIUrl":"https://doi.org/10.1002/dvdy.70027","url":null,"abstract":"<p><strong>Background: </strong>The brown anole, Anolis sagrei, has emerged as a representative squamate species for developmental studies during the past decades. Novel functional tools have been established to manipulate embryogenesis through genome editing or the introduction of small molecule inhibitors, and their effective use requires a thorough understanding of early anole embryogenesis. To enable precise and reproducible staging of anole embryos, we need knowledge of the progression of anole embryogenesis and morphogenesis. While post-oviposition development has been described, the pre-oviposition period remains to be explored.</p><p><strong>Results: </strong>We provide the first staging series of pre-oviposition development for the brown anole. Analyzing the follicles and embryos through brightfield imaging, SEM, STEM, histology, and DAPI staining, we define 26 distinct developmental stages. Furthermore, we followed heart development, neural crest cell migration, and central nervous system development using immunofluorescence analyses and provide new comparative insights into the morphogenesis of each of these organ systems.</p><p><strong>Conclusions: </strong>Our dataset reveals that peri-gastrulation morphogenesis up to the initiation of neurulation diverges significantly from chick, the common representative model of reptile embryogenesis. With this study, we establish the brown anole as a squamate model organism for cross-clade evolutionary studies of early embryogenesis.</p>","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143977491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamic expression of lamin B1 during adult neurogenesis in the vertebrate brain.","authors":"Diana Zhilina, Lizbeth A Bolaños Castro, Juan Sebastian Eguiguren, Sara Zocher, Anne Karasinsky, Dimitri Widmer, Alexandre Espinós, Victor Borrell, Michael Brand, Kyoko Miura, Oliver Zierau, Maximina H Yun, Tomohisa Toda","doi":"10.1002/dvdy.70023","DOIUrl":"https://doi.org/10.1002/dvdy.70023","url":null,"abstract":"<p><strong>Background: </strong>In mammals, specific brain regions such as the dentate gyrus (DG) of the hippocampus and the subventricular zone (SVZ) of the lateral ventricles harbor adult neural stem/progenitor cells (ANSPCs) that give rise to new neurons and contribute to structural and functional brain plasticity. In contrast, other vertebrates such as salamanders and zebrafish exhibit a widely distributed neurogenic niches throughout the brain, suggesting a greater neurogenic capacity in adulthood. However, the mechanisms underlying this divergence in neurogenic potential among vertebrates remain elusive. To address this, we examined the expression dynamics of a critical epigenetic regulator for the long-term maintenance of murine ANSPCs, lamin B1, during adult neurogenesis across the vertebrate spectrum.</p><p><strong>Results: </strong>Lamin B1 expression patterns during adult neurogenesis are conserved among mammals including mouse, naked mole-rat, and ferret. However, these patterns differ between mammals and anamniotes. In mammals, neural stem cells and neuroblasts exhibited higher lamin B1 levels, and differentiated neurons possessed lower lamin B1 levels. On the other hand, anamniotes showed the opposite patterns of lamin B1 expression, with higher levels in neurons compared to stem cells.</p><p><strong>Conclusions: </strong>Our study shows that the lamin B1 expression pattern during adult neurogenesis differs between species, and that changes in lamin B1 protein sequence may contribute to the differences in lamin B1 expression patterns. This study highlights potential differences in cell-autonomous epigenetic regulation in the maintenance of ANSPC pools in the adult brain among species.</p>","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143991519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}