Developmental Dynamics最新文献

{"title":"Molecular insights into the role of Wnt signaling in supernumerary maxillary incisor formation.","authors":"Resmi Raju, Jeremie Oliver Piña, Aye Chan Myo, Parna Chattaraj, Taishi Komori, Mitsuaki Ono, Ziyi Wang, Didier Portilla, Rena N D'Souza","doi":"10.1002/dvdy.70190","DOIUrl":"https://doi.org/10.1002/dvdy.70190","url":null,"abstract":"<p><strong>Background: </strong>Sclerostin domain-containing 1 (SOSTDC1) deficiency enhances BMP and Wnt signaling, causing supernumerary teeth formation. Competing hypotheses of this formation at different sites have been proposed, and the underlying molecular mechanisms and cell types remain poorly understood.</p><p><strong>Results: </strong>We examined Sostdc1 knockout effects on spatiotemporal expression of Wnt pathway genes in developing maxillary incisors. In wild type mice, we observed an inverse spatiotemporal expression of Sostdc1 and Wnt10a. The knockout caused ectopic expression of Wnt10a, Wnt6, Lrp4, Dkk1, and Sost and produced a distinct subpopulation of mesenchymal cells. Their spatial transcriptomic analysis and validation of marker genes by in situ mRNA hybridization suggested an unexpected mix of osteogenic and odontogenic differentiation. These cells evolved at the rudimentary labial incisor outgrowth at ~E15.5. At E16.5, their population expanded toward the lingual cervical loop, where a separate supernumerary tooth formed at E17.5.</p><p><strong>Conclusion: </strong>The loss of Wnt and BMP signaling inhibition by SOSTDC1 causes aberrant mixed osteogenic and odontogenic differentiation of mesenchymal cells, contributing first to the labial outgrowth and then to the formation of the lingual supernumerary tooth. We hypothesize that this aberrant differentiation underlies the pathology, yet its direct functional role in the supernumerary tooth formation remains to be established.</p>","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149105506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The emerging role of ER-localized TMEM proteins in neurodevelopmental disorders.","authors":"Shreni Priyadarshini, Rishi Kumar, Debdeep Dutta","doi":"10.1002/dvdy.70189","DOIUrl":"https://doi.org/10.1002/dvdy.70189","url":null,"abstract":"<p><p>Neurodevelopmental disorders (NDDs) comprise a spectrum of conditions that impair brain development and nervous system function, resulting in lifelong cognitive, behavioral, and motor disabilities. Globally, more than 3% of children are affected by NDDs. Although advances in next-generation sequencing technology have identified many disease-associated genes, the cellular and molecular mechanisms underlying NDD pathogenesis remain poorly understood. Recent genomic and clinical studies have highlighted the transmembrane (TMEM) family of proteins as emerging contributors to NDDs. TMEM proteins represent a structurally and functionally diverse group that localize to membranes of various cellular organelles, including the endoplasmic reticulum (ER). Several ER-resident TMEM proteins have been increasingly implicated in neurodevelopmental impairments, suggesting a critical role for ER-associated processes in brain development and function. In this review, we describe the key ER-resident TMEM proteins and their association with neurodevelopmental disorders. We discuss the known cellular functions of these proteins, summarize evidence linking their dysfunction to neurodevelopmental phenotypes, and highlight emerging mechanisms by which ER-resident TMEM proteins may contribute to NDD pathogenesis. Finally, we outline key knowledge gaps and discuss how elucidating the functions of these proteins may provide new insights into disease etiology.</p>","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149105446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Embryonic and larval development of the Pacific saury Cololabis saira: Distinctive characteristics of a rapidly growing beloniform fish.","authors":"Rie Kusakabe, Shinya Yamauchi, Shigehiro Kuraku","doi":"10.1002/dvdy.70188","DOIUrl":"https://doi.org/10.1002/dvdy.70188","url":null,"abstract":"<p><strong>Background: </strong>Pacific saury Cololabis saira is one of the important food resources drawing attention for its recent rapid decline of catch. Their life cycle and embryonic development have been largely unknown. It is important to clarify how the habitat and reproduction of this species have been affected by the global changes of the aquatic environment.</p><p><strong>Results: </strong>We obtained fertilized eggs of C. saira by spontaneous spawning and artificial fertilization and observed the embryonic development up to larval stages. Embryonic stages are documented with major periods of developmental events: cleavage, gastrulation (epiboly), and somitogenesis and organogenesis. Remarkably, segmentation of somites starts in the middle of epiboly, unlike other well-documented teleost species such as zebrafish and medaka. Morphological changes in the larval stage up to feeding juvenile are also described. Growth speed of larval Pacific saury is dramatically rapid in comparison to closely related beloniform fish such as medaka.</p><p><strong>Conclusions: </strong>In comparison to medaka, early embryogenesis of saury proceeds relatively slowly, but is followed by an earlier onset of somitogenesis, revealing a distinct developmental timetable. Despite reaching adult body length exceeding 20 cm in only half a year, the developmental basis of the remarkable growth of saury remains unclear. The developmental staging framework established here provides a foundation for future studies investigating the molecular mechanisms underlying saury development, as well as the effects of environmental factors, including ocean warming, on embryonic development and early life-history traits.</p>","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2026-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149016057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Meet the Editorial Team. An interview with Angelo Iulianella, Assistant Editor, Dalhousie University Faculty of Medicine, Canada","authors":"Paul Trevorrow,&nbsp;Angelo Iulianella","doi":"10.1002/dvdy.70139","DOIUrl":"10.1002/dvdy.70139","url":null,"abstract":"&lt;p&gt;I had quite an unusual journey into science. I was born and raised in Montreal and went to a public school that didn't particularly emphasize science, but for reasons I can't fully explain, I was always drawn to it. Growing up in a part of the suburbs that was still being developed, I spent a lot of time exploring the wooded areas around our house. I think that early curiosity about the natural world sparked my interest in biology.&lt;/p&gt;&lt;p&gt;By my final year of high school, I knew I wanted to study biology, so I went on to McGill University and specialized in molecular genetics. I completed an undergraduate research project testing the idea of selfish DNA as a mechanism for evolutionary innovation. This reinforced my interest in research as career, which led me straight into graduate studies. I stayed in Montreal for my PhD, working at the Clinical Research Institute of Montreal (IRCM) with Doctor David Lohnes, who had trained with Pierre Chambon, the founder of the Institute for Genetics and Cellular and Molecular Biology (INSERM) in Strasbourg, France. David did his postdoctoral research at INSERM during the exciting early days of knockout mouse technology. He had been part of the team that generated some of the first knockout mouse models, including those for the Retinoic Acid Receptors (RARs).&lt;/p&gt;&lt;p&gt;I joined David Lohnes' lab at IRCM after approaching several professors for potential graduate positions, and David happened to be taking on students at the time. My PhD work focused on the role of RARs during development. We engineered chimeric RARs in mice, which was innovative at the time, and I learned techniques such as blastocyst injection and embryonic stem cell culture—initially by studying the standard “mouse Bible” of techniques (Manipulating the Mouse Embryo, CSHL Press) and with careful guidance from David. My work explored craniofacial, skeletal, and cardiac development. One of my key findings as a graduate student was identifying a molecular regulatory network involving Wnt3A, Brachyury (T), and CDX genes as downstream targets of Retinoic Acid signaling in axial elongation. That work became one of my most significant publications (in &lt;i&gt;Developmental Biology&lt;/i&gt;), which continues to be cited, especially now with renewed interest driven by human gastruloid models of embryo segmentation.&lt;/p&gt;&lt;p&gt;After completing my PhD in 2002, I wasn't entirely sure which direction to take next. I interviewed at several places, one of which was a new research institute being established by Robb Krumlauf. I had always been interested in neural patterning, and Robb was a leader in the field, especially in hindbrain and Hox gene research. I was initially excited about the idea of going to the renowned Mill Hill labs in London, where Robb's lab was situated, but he informed me he was moving to Kansas City to head the newly created Stowers Institute for Medical Research. He invited me to visit, and although the labs were still under construction—hard hats req","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":"255 9","pages":"842-844"},"PeriodicalIF":1.5,"publicationDate":"2026-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/dvdy.70139","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147940545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of mutant TIE2 p.L914F during mouse development causes embryonic lethality and defects in vascular remodeling","authors":"Lindsay J. Bischoff,&nbsp;Sandra Schrenk,&nbsp;Kara Soroko,&nbsp;Chhiring Sherpa,&nbsp;Ashok Arasu,&nbsp;Damien Reynaud,&nbsp;Elisa Boscolo","doi":"10.1002/dvdy.70087","DOIUrl":"10.1002/dvdy.70087","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Sporadic venous malformation (VM) is associated with the hyperactivating p.L914F mutation in TIE2, a receptor tyrosine kinase essential for vascular development. This mutation is not found in hereditary VM, suggesting incompatibility with life when expressed during early vascular development. Therefore, we utilized a genetic mouse model that expresses TIE2 p.L914F to determine its phenotypical effects during development.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p><i>B6-Tg(Rosa26-TIE2</i>^<i>L914F</i><i>)</i>^<i>EBos</i> (<i>TIE2</i>^<i>L914F</i>) mice were generated and then validated for the presence of the transgene. The constitutive endothelial-specific <i>Tie2-Cre</i> line was used to activate expression of the mutant gene during early embryonic development. <i>Tie2-Cre;TIE2</i>^<i>L914F</i> embryos experienced lethality at approximately embryonic day (E)9.5. Three-dimensional imaging of embryos and yolk sacs revealed impaired vascular remodeling in mutant animals, resulting in malformed vasculature with disorganized, dilated, and non-functional blood vessels. The abnormal yolk sac vascular phenotype was not associated with total loss of erythroid cells or increased cell proliferation.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The <i>TIE2</i>^<i>L914F</i> mice used in this study represent a novel genetic model of TIE2 p.L914F-driven vascular disease. This study provides the first experimental evidence that this mutation is incompatible with early prenatal development due to its deleterious effects on the vasculature, illustrating the vital role of balanced TIE2 signaling during vessel development and remodeling.</p>\u0000 </section>\u0000 </div>","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":"255 9","pages":"896-914"},"PeriodicalIF":1.5,"publicationDate":"2026-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12853423/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145274189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The segregation of Calb1, Calb2, and Prph neurons reveals distinct and mixed neuronal populations and projections to hair cells in the inner ear and central nuclei","authors":"Jeong Han Lee,&nbsp;Ebenezer N. Yamoah,&nbsp;Jennifer Kersigo,&nbsp;Karen Elliott,&nbsp;Niya LaRoda,&nbsp;Gabriela Pavlinkova,&nbsp;Bernd Fritzsch","doi":"10.1002/dvdy.70093","DOIUrl":"10.1002/dvdy.70093","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Knockin mouse models expressing calbindin (<i>Calb1</i>), calretinin (<i>Calb2</i>), and peripherin (<i>Prph</i>) exhibit changes in hair cells (HCs), spiral ganglion neurons (SGN), vestibular ganglion neurons (VGNs), and their central projections.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Developing cristae HCs show strong Calb1-positive expression, but adult HCs are mainly Calb2-positive. Utricle and saccule initially have Calb2-positive HCs and later develop Calb1-positive HCs in the striola region. Inner hair cells (IHCs) and outer hair cells (OHCs) in the cochlea express Calb2 early on. Calb1 expression in OHCs overlaps with Calb2; the expression of Myo7a, Calb1, and Calb2 reaches the apex later. SGNs and VGNs exhibit distinct Calb1 and Calb2 patterns but include a subpopulation with mixed expression. Central fibers are Calb1- and Calb2-positive early in the developing cochlear nuclei (CN) and vestibular nuclei (VN) but remain highly Prph-positive. VGNs innervate the lateral and VN, which are positive for Calb2 and Prph. Distinct Calb1-positive neurons overlap with the anterior (A) and ventral (V) cochlear nuclei (AVCN, PVCN) with Calb2, while the dorsal cochlear nucleus (DCN) shows segregation of Calb2 and Calb1.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>We offer insights into the timing of how neuronal identity and connectivity are regulated in the auditory and vestibular systems, as shown by the expression of Calb1, Calb2, and Prph.</p>\u0000 </section>\u0000 </div>","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":"255 9","pages":"845-866"},"PeriodicalIF":1.5,"publicationDate":"2026-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12907763/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145400166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Orthodentine-osteodentine and osteocytic-anosteocytic transition inferred from Silurian stem chondrichthyan dermal jawbones with ever-white teeth overgrown by brown denticles.","authors":"Jia-Cih Kang, Henning Blom, Tiiu Märss, Donglei Chen","doi":"10.1002/dvdy.70184","DOIUrl":"https://doi.org/10.1002/dvdy.70184","url":null,"abstract":"<p><strong>Background: </strong>The dentigerous jawbones of a 423-million-year-old stem chondrichthyan (ischnacanthid acanthodian) carry a characteristic combination of multi-row non-shedding white teeth with brownish overgrowing denticles. The color distinction makes it a good model for observing the interaction between two odontode lineages, but the nature of the opaque white material and its relationship to other tissues is unknown.</p><p><strong>Results: </strong>Synchrotron microtomography demonstrates that the white tissue is a lightly mineralized orthodentine. By contrast, the orthodentine on the denticles is highly mineralized and almost vitreous. The brownish color of the denticles is from the osteodentine that fills the pulp of both teeth and denticles. The continuation of dentine tubules through orthodentine and osteodentine indicates their production by the same population of odontoblasts. The teeth are added sequentially, while the same generation of denticles is added synchronously, both associated with bone apposition. The overgrowth of denticles at the growing front of a tooth row will terminate its tooth addition, and the timing of this event determines the number and length of the tooth rows. Osteocyte lacunae in the latest bone layers appear to be infilled by bone matrix, which explains the scarcity of lacunae in the matured bone layers.</p><p><strong>Conclusions: </strong>Heterochronic shift of the mineralization processes could be a potential mechanism to produce the peculiar white tissue. It suggests the invention of novel dental tissues may only require modification of the developmental process, which may provide an explanation for why vertebrates have already acquired a great diversity of dental tissues during their early evolution.</p>","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2026-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148954945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cryopreservation of sea urchin (Lytechinus pictus) embryos and development through metamorphosis","authors":"Victor D. Vacquier,&nbsp;Amro Hamdoun","doi":"10.1002/dvdy.70081","DOIUrl":"10.1002/dvdy.70081","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Sea urchins have contributed to knowledge of fertilization, embryonic development, and cell physiology for 150 years. Their evolutionary position, as basal deuterostomes, and their long background in developmental biology motivate establishing a genetically enabled sea urchin species. Because of its relatively short generation time of 4–6 months and ease of culture, our lab has focused on the California sea urchin <i>Lytechinus pictus</i> as a multigenerational model and produced knockout and transgenic lines using this species. To ensure that diverse genetic lines can be preserved, methods must be developed to cryopreserve gametes and embryos. We have previously reported methods for cryopreservation of sperm, but robust methods to preserve embryos remain lacking.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Here, we describe a relatively simple method to cryopreserve late gastrulae embryos of <i>L. pictus.</i> Importantly, we show that, after thawing and culturing, the embryos progress through larval development, undergo metamorphosis, and yield juvenile adults, indicating the method is robust.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The cryopreservation of embryos is an important advance that will facilitate the biobanking, sharing, and long-term preservation of diverse genetic lines. This method may also eventually prove useful for cryopreservation of embryos of other marine invertebrates.</p>\u0000 </section>\u0000 </div>","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":"255 9","pages":"915-919"},"PeriodicalIF":1.5,"publicationDate":"2026-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145274136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial highlights","authors":"Paul A. Trainor","doi":"10.1002/dvdy.70185","DOIUrl":"https://doi.org/10.1002/dvdy.70185","url":null,"abstract":"&lt;p&gt;Every organism is a model organism for understanding development, evolution, disease, and regeneration, and we have only begun to scratch the surface of the interdisciplinary genetic, molecular, cellular, and developmental mechanisms that regulate these biological processes. These “Highlights” denote exciting advances recently reported in &lt;i&gt;Developmental Dynamics&lt;/i&gt; that illustrate the complex dynamics of developmental biology.&lt;/p&gt;&lt;p&gt;&lt;b&gt;Meet the Editorial Team&lt;/b&gt; “An interview with Angelo Iulianella, assistant editor, Dalhousie University” by Paul Trevorrow and Angelo Iulianella.&lt;span&gt;&lt;sup&gt;1&lt;/sup&gt;&lt;/span&gt; Dr Iulianella has had a long-standing interest in the underlying mechanisms guiding the formation of complex anatomies. This began with studies of axial elongation and patterning and skeletal development during his PhD, followed by analyses of neuron formation and signaling and patterning in the nervous system during his post-doctoral training. Dr Iulianella established his research laboratory focused on uncovering the mechanisms regulating neurogenesis and neuronal connectivity and has recently focused on a protein unique to vertebrates, called MLLT11. MLLT11 represents a new type of cytoskeletal-interacting protein that is critical for neuronal migration and may also be important in the pathogenesis of cancer. Dr Iulianella's long-term fascination with how neural stem cells decide which cell types to become, how they migrate, and how their movements contribute to the complex architecture of the nervous system continues to reveal novel regulators of neural development and identify molecular pathways implicated in the pathogenesis of neurodevelopmental disorders.&lt;/p&gt;&lt;p&gt;&lt;b&gt;Hearing and Neurogenesis in the Inner Ear&lt;/b&gt; “The segregation of Calb1, Calb2, and Prph neurons reveals distinct and mixed neuronal populations and projections to hair cells in the inner ear and central nuclei” by Jeong Han Lee, Ebenezer Yamoah, Jennifer Kersigo, Karen Elliott, Niya LaRoda, Gabriela Pavlinkova, and Bernd Fritzsch.&lt;span&gt;&lt;sup&gt;2&lt;/sup&gt;&lt;/span&gt; The complex perception of sound and head movements, together with responses to body position and gravity, depends on a diverse collection of cochlear and vestibular hair cells, spiral ganglion neurons, and vestibular ganglion neurons. The authors used mouse models expressing &lt;i&gt;Calb1-mCherry, Calb2-eGFP&lt;/i&gt;, and &lt;i&gt;Prph-eGFP&lt;/i&gt; to determine the dynamics of neuronal identity in auditory and vestibular hair cells, sensory neurons, and their central projections extending from the auditory and vestibular ganglia to the brainstem nuclei. The authors uncovered novel interactions between cochlear and vestibular hair cells, the spiral and vestibular ganglia, and the cochlear and vestibular nuclei. The work underscores the importance of age-dependent protein expression, offering crucial insights into the temporal regulation of neuronal and hair cell identity and connectivity in the auditory and vestibular systems, which are esse","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":"255 9","pages":"840-841"},"PeriodicalIF":1.5,"publicationDate":"2026-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/dvdy.70185","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148943362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biophysical processes of morphogenesis in lizard lungs","authors":"Kaleb Hill,&nbsp;Aaron H. Griffing,&nbsp;Michael A. Palmer,&nbsp;Bezia Lemma,&nbsp;Aria S. Lupo,&nbsp;Tony Gamble,&nbsp;Natalia A. Shylo,&nbsp;Andrej Košmrlj,&nbsp;Paul A. Trainor,&nbsp;Celeste M. Nelson","doi":"10.1002/dvdy.70097","DOIUrl":"10.1002/dvdy.70097","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>The lungs of squamate reptiles (lizards and snakes) are highly diverse, exhibiting single chambers, multiple chambers, transitional forms with two to three chambers, along with a suite of other anatomical features, including finger-like epithelial projections into the body cavity known as diverticulae. During embryonic development of the simple, sac-like lungs of anoles, the epithelium is pushed through the openings of a pulmonary smooth muscle mesh by the forces of luminal fluid pressure. This process of stress ball morphogenesis generates the faveolar epithelium typical of squamate lungs.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Here, we compared embryonic lung development in brown anoles, leopard geckos, and veiled chameleons to determine if stress ball morphogenesis is conserved across squamates and to understand the physical processes that generate transitional-chambered lungs with diverticulae. We found that epithelial protrusion through the holes in a pulmonary smooth muscle mesh is conserved across squamates. Surprisingly, however, we found that luminal inflation is not conserved. Instead, experimental and computational evidence suggests that leopard geckos and veiled chameleons may generate their faveolae via epithelial folding downstream of epithelial proliferation. Our data also suggest that the transitional chambers and diverticulae of veiled chameleon lungs may develop via apical constriction, a process known to be crucial for airway branching in the bird lung.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Distinct morphogenetic mechanisms generate epithelial diversity in squamate lungs, which may underpin their species-specific physiological and ecological adaptations.</p>\u0000 </section>\u0000 </div>","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":"255 9","pages":"867-880"},"PeriodicalIF":1.5,"publicationDate":"2026-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13553195/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145512001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}