Developmental Dynamics最新文献_第2页

DNA repair during embryonic epidermal stratification. 胚胎表皮分层过程中的DNA修复。

IF 2 3区生物学

Developmental Dynamics Pub Date : 2025-05-29 DOI: 10.1002/dvdy.70046

Fumiya Meguro, Katsushige Kawasaki, Yoshito Kakihara, Maiko Kawasaki, Makoto Fukushima, Finsa Tisna Sari, Vanessa Utama, Alex Kesuma, Jun Nihara, Takehisa Kudo, Akira Fujita, Kaya Ichikawa, Kazuaki Osawa, Takeyasu Maeda, Koichi Tabeta, Makio Saeki, Atsushi Ohazama

{"title":"DNA repair during embryonic epidermal stratification.","authors":"Fumiya Meguro, Katsushige Kawasaki, Yoshito Kakihara, Maiko Kawasaki, Makoto Fukushima, Finsa Tisna Sari, Vanessa Utama, Alex Kesuma, Jun Nihara, Takehisa Kudo, Akira Fujita, Kaya Ichikawa, Kazuaki Osawa, Takeyasu Maeda, Koichi Tabeta, Makio Saeki, Atsushi Ohazama","doi":"10.1002/dvdy.70046","DOIUrl":"https://doi.org/10.1002/dvdy.70046","url":null,"abstract":"Background: Genomes are constantly exposed to a myriad of DNA-damaging agents. Robust DNA repair mechanisms protect DNA by removing or tolerating damage. However, it remains unclear whether these mechanisms are required for organogenesis.Results: Multiple epithelial layers are essential for skin function, including body protection. The epidermis is initiated as a single layer and then stratifies in utero. Stratification did not occur in mice with epithelial conditional deletion of the DNA repair molecule Reptin (Reptinfl/fl;K14Cre). DNA damage was observed in the mutant epidermis but not in the wild-type epidermis. The mutant epidermis also showed reduced cell proliferation and upregulated p53 expression. Stratification was restored when p53 was deleted in the Reptin mutant mice by generating Reptin and p53 double mutant mice (Reptinfl/fl;K14Cre;p53-/-).Conclusion: In the wild-type epidermis, DNA is likely damaged at the initiation of embryonic stratification and promptly repaired by DNA repair mechanisms involving Reptin.","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144172914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Streamlining asymmetry quantification in fetal mouse imaging: A semi-automated pipeline supported by expert guidance. 胎儿小鼠成像中的流线型不对称量化：专家指导下的半自动流水线。

IF 2 3区生物学

Developmental Dynamics Pub Date : 2025-05-27 DOI: 10.1002/dvdy.70028

S M Rolfe, D Mao, A M Maga

{"title":"Streamlining asymmetry quantification in fetal mouse imaging: A semi-automated pipeline supported by expert guidance.","authors":"S M Rolfe, D Mao, A M Maga","doi":"10.1002/dvdy.70028","DOIUrl":"10.1002/dvdy.70028","url":null,"abstract":"Background: Asymmetry is a key feature of numerous developmental disorders and in phenotypic screens is often used as a readout for environmental or genetic perturbations. A better understanding of the genetic basis of asymmetry and its relationship to disease susceptibility will help unravel the complex genetic and environmental factors and their interactions that increase risk in a range of developmental disorders. Large-scale imaging datasets offer opportunities to work with sample sizes necessary to detect and quantify differences in morphology beyond severe deformities but also pose challenges to manual phenotyping protocols.Results: We introduce a tool for quantifying asymmetry in 3D images and apply it to explore the role of genes contributing to abnormal asymmetry by deep phenotyping 3D fetal microCT images from knockout strains acquired as part of the Knockout Mouse Phenotyping Program. Four knockout strains: Ccdc186, Acvr2a, Nhlh1, and Fam20c were identified with highly significant asymmetry in craniofacial regions, making them good candidates for further analysis.Conclusion: In this work, we demonstrate an open-source, semi-automated tool to quantify the asymmetry of craniofacial structures that integrates expert anatomical knowledge. This tool can detect abnormally asymmetric phenotypes in fetal mice to explore the relationship between facial asymmetry, perturbed development, and developmental instability.","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144149685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differences in palatal shelf epithelial stiffness between the lingual/nasal and buccal/oral surfaces during palatal shelf elevation in developing mice. 发育中小鼠腭架抬高过程中舌/鼻和颊/口腔表面腭架上皮硬度的差异。

IF 2 3区生物学

Developmental Dynamics Pub Date : 2025-05-23 DOI: 10.1002/dvdy.70044

Arata Nagasaka, Yasuhiko Bando, Miyuki Toda-Fujii, Go Onozawa, Kaito Suzuki, Takaki Miyata, Osamu Amano

{"title":"Differences in palatal shelf epithelial stiffness between the lingual/nasal and buccal/oral surfaces during palatal shelf elevation in developing mice.","authors":"Arata Nagasaka, Yasuhiko Bando, Miyuki Toda-Fujii, Go Onozawa, Kaito Suzuki, Takaki Miyata, Osamu Amano","doi":"10.1002/dvdy.70044","DOIUrl":"https://doi.org/10.1002/dvdy.70044","url":null,"abstract":"Background: During secondary palate formation, bilateral palatal shelves grow vertically to a horizontal position. This morphological change of the palatal shelves, defined as the palatal shelf elevation, occurs from embryonic day (E)-13.5 to E14 in mice. Palatal shelves show regional differences in elevation patterns along the anterior-posterior (AP) axis; however, the underlying mechanisms remain unclear. Material properties of the lingual/nasal and buccal/oral surfaces, especially stiffness, possibly contribute to different elevation patterns.Results: Indentation test using atomic force microscopy was performed to measure the stiffness at the epithelial surface of the palatal shelf. Measurement of palatal shelf stiffness along the AP axis before and after elevation revealed that the lingual/nasal surface was softer than the buccal/oral surface in the posterior region before elevation and that the palatal shelf was stiffer after elevation than before elevation. Moreover, the thickness of epithelial cells on the lingual/nasal side was lower than that on the buccal/oral side before elevation.Conclusion: Overall, our results suggest that epithelial cell thickness affects epithelial surface stiffness, causing regional differences in elevation patterns.","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144132167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pss knockdown in the midgut causes growth retardation in Drosophila similar to that in human LMHD. Pss在中肠中的敲低导致果蝇的生长迟缓，类似于人类LMHD。

IF 2 3区生物学

Developmental Dynamics Pub Date : 2025-05-22 DOI: 10.1002/dvdy.70039

Kwan-Young Kim, You-Lim Hwang, Sunwoo Yeom, Seung-Hae Kwon, Sang-Hak Jeon

{"title":"Pss knockdown in the midgut causes growth retardation in Drosophila similar to that in human LMHD.","authors":"Kwan-Young Kim, You-Lim Hwang, Sunwoo Yeom, Seung-Hae Kwon, Sang-Hak Jeon","doi":"10.1002/dvdy.70039","DOIUrl":"https://doi.org/10.1002/dvdy.70039","url":null,"abstract":"Background: Phosphatidylserine synthase (PSS), localized in the mitochondrial membrane, synthesizes phosphatidylserine. In humans, mutations in Pss lead to Lenz-Majewski hyperostotic dwarfism, a disorder affecting growth and development. The effects of Pss mutations on the growth of Drosophila melanogaster are not fully known. Hence, this study was conducted to investigate the effects of Pss knockdown on the growth and development of D. melanogaster.Results: Enterocyte (EC)-specific Pss knockdown resulted in reduced cell size in the gut via reduced Akt signaling. EC-specific Pss knockdown was associated with a decrease in gut size, a change in gut pH, and reduced food intake. These abnormalities affected normal nutrient metabolism in larvae, leading to decreased secretion of Drosophila insulin-like peptides. Consequently, the reduced systemic Akt signaling at the organismal level resulted not only in impaired gut growth but also in abnormal organismal growth and development.Conclusion: These findings highlight the significant role of the Pss gene in the growth and development of D. melanogaster.","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144118587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protein turnover downstream of the Nipbl/CRL4 axis contributes to abnormal development in zebrafish embryos. Nipbl/CRL4轴下游的蛋白质周转有助于斑马鱼胚胎的异常发育。

IF 2 3区生物学

Developmental Dynamics Pub Date : 2025-05-21 DOI: 10.1002/dvdy.70037

Annie C Sanchez, Niusha Banoukh, Fiona Mensching, Robert V Skibbens, M Kathryn Iovine

{"title":"Protein turnover downstream of the Nipbl/CRL4 axis contributes to abnormal development in zebrafish embryos.","authors":"Annie C Sanchez, Niusha Banoukh, Fiona Mensching, Robert V Skibbens, M Kathryn Iovine","doi":"10.1002/dvdy.70037","DOIUrl":"https://doi.org/10.1002/dvdy.70037","url":null,"abstract":"Background: Mutations in cohesins cause cohesinopathies such as Cornelia de Lange Syndrome (CdLS) and Roberts Syndrome (RBS). Prior findings demonstrate that Esco2 (a cohesin activator) and Smc3 (a core cohesin subunit) regulate the CRL4 E3 ubiquitin ligase. SMC3 mutations, however, account for a small percentage of CdLS. Here, we test whether NIPBL, which when mutated is responsible for 65% of CdLS cases, also regulates CRL4.Results: We report that Nipbl knockdown in zebrafish embryos produces developmental abnormalities and reduces the transcription of ddb1, which encodes a key component of CRL4 E3 ligase. The severity of phenotypes in Nipbl knockdown embryos is partially rescued by exogenous ddb1 mRNA, demonstrating that CRL4 ligase function is downstream of Nipbl. These findings suggest that aberrant accumulation of CRL4 ligase substrates contributes to developmental abnormalities. To test this model, we identified candidate CRL4 substrates in zebrafish embryos by LC-MS. The results reveal that elevated expression of one of these candidates, pparαa, is sufficient to produce developmental defects in zebrafish embryos.Conclusions: Nipbl impacts CRL4 ligase activity via regulation of ddb1 expression. We provide evidence that the aberrant accumulation of substrates is sufficient to produce developmental abnormalities consistent with those observed in RBS and CdLS models.","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144110222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Downstream branches of receptor tyrosine kinase signaling act interdependently to shape the face. 受体酪氨酸激酶信号的下游分支相互依赖地起作用，形成面部。

IF 2 3区生物学

Developmental Dynamics Pub Date : 2025-05-20 DOI: 10.1002/dvdy.70041

Nicholas Hanne, Diane Hu, Marta Vidal-García, Charlie Allen, M Bilal Shakir, Wei Liu, Benedikt Hallgrímsson, Ralph Marcucio

{"title":"Downstream branches of receptor tyrosine kinase signaling act interdependently to shape the face.","authors":"Nicholas Hanne, Diane Hu, Marta Vidal-García, Charlie Allen, M Bilal Shakir, Wei Liu, Benedikt Hallgrímsson, Ralph Marcucio","doi":"10.1002/dvdy.70041","DOIUrl":"10.1002/dvdy.70041","url":null,"abstract":"Background: Previously we found that increasing fibroblast growth factor (FGF) signaling in the neural crest cells within the frontonasal process (FNP) of the chicken embryo caused dysmorphology that was correlated with reduced proliferation, disrupted cellular orientation, and lower MAPK activation but no change in PLCγ and PI3K activation. This suggests RTK signaling may drive craniofacial morphogenesis through specific downstream effectors that affect cellular activities. In this study we inhibited three downstream branches of RTK signaling to determine their role in regulating cellular activities and how these changes affect morphogenesis of the FNP.Results: Small molecule inhibitors of MEK1/2, PI3K, and PLCγ were delivered individually and in tandem to the right FNP of chicken embryos. All treatments caused asymmetric proximodistal truncation on the treated side and a mild expansion on the untreated side compared to DMSO control treated FNPs. Inhibiting each pathway caused similar decreased proliferation and disrupted cellular orientation, and only mildly increased apoptosis.Conclusions: Since RTK signaling is a ubiquitous and tightly regulated biochemical system, we conclude that the downstream pathways are robust to developmental perturbation through redundant signaling systems.","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144110221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The endocannabinoid system regulates both ependymoglial and neuronal cell responses to a tail amputation in the axolotl. 内源性大麻素系统调节室管膜胶质细胞和神经元细胞对蝾螈尾巴截肢的反应。

IF 2 3区生物学

Developmental Dynamics Pub Date : 2025-05-16 DOI: 10.1002/dvdy.70035

Michael Tolentino, Sarah E Walker, Gaynor E Spencer, Robert Carlone

{"title":"The endocannabinoid system regulates both ependymoglial and neuronal cell responses to a tail amputation in the axolotl.","authors":"Michael Tolentino, Sarah E Walker, Gaynor E Spencer, Robert Carlone","doi":"10.1002/dvdy.70035","DOIUrl":"https://doi.org/10.1002/dvdy.70035","url":null,"abstract":"Background: The endocannabinoid system is a neuromodulatory system implicated in cellular processes during both development and regeneration. The Mexican axolotl, one of only a few vertebrates capable of central nervous system regeneration, was used to examine the role of the endocannabinoid system in the regeneration of the tail and spinal cord following amputation.Results: The endocannabinoid receptor CB1 was upregulated in the regenerating axolotl spinal cord by 4 hours following tail amputation, and this upregulation persisted for at least 14 days. The endocannabinoid receptor CB2 was upregulated later, between 7 and 14 days after tail amputation. Both CB1 and CB2 were located in ependymoglia and neurons within the regenerating spinal cord. Treatment with inverse agonists to inhibit CB1 (AM251) or CB2 (AM630) inhibited spinal cord and tail regeneration. During the first 7 days after injury, CB1 and CB2 expression was also necessary for the proliferation of ependymoglial cells and the regeneration of axons into the newly regenerated tail tissue. However, only CB1 was necessary for the differentiation of ependymoglia into immature neurons.Conclusions: These studies are the first to examine the role of the endocannabinoid system during spinal cord regeneration in a regeneration-competent vertebrate.","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144076812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in culturing of the sea star Patiria miniata. 海星的培养研究进展。

IF 2 3区生物学

Developmental Dynamics Pub Date : 2025-05-15 DOI: 10.1002/dvdy.70040

Vanessa Barone, Luisa Coronado, Deka Ismail, Sareen Fiaz, Deirdre C Lyons

{"title":"Advances in culturing of the sea star Patiria miniata.","authors":"Vanessa Barone, Luisa Coronado, Deka Ismail, Sareen Fiaz, Deirdre C Lyons","doi":"10.1002/dvdy.70040","DOIUrl":"https://doi.org/10.1002/dvdy.70040","url":null,"abstract":"Background: The use of the sea star Patiria miniata as a model system has produced groundbreaking advances in a disparate set of biomedical research fields, including embryology, immunology, regeneration, cell biology, and evolution of development. Nonetheless, the life cycle of P. miniata has not yet been closed in the laboratory, precluding the generation of stable transgenic and mutant lines, which would greatly expand the toolset for experimentation with this model system. Rearing P. miniata in the laboratory has been challenging due to limited knowledge about metamorphosis cues, feeding habits of juveniles, and their relatively long generation time.Results: Here we report protocols to rear P. miniata embryos through sexual maturity in a laboratory setting. We provide detailed staging of early embryonic development at different temperatures, and show that larvae can be raised to competence in as little as 15 days. We find that retinoic acid induces metamorphosis effectively and present methods to rear juveniles on commercially available foods. We show that in a flow-through system, juveniles double in size every 2 months and reach sexual maturity in approximately 2 years.Conclusions: We report the first example of P. miniata raised through sexual maturity in a laboratory setting, paving the way for the generation of stable mutant sea star lines.","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144076743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Therapeutic potential of stem cells in colorectal cancer management: Current trends and future prospects. 干细胞在结直肠癌治疗中的治疗潜力：当前趋势和未来前景。

IF 2 3区生物学

Developmental Dynamics Pub Date : 2025-05-13 DOI: 10.1002/dvdy.70042

Manya D Desai, Namrata B Parmar, Isha T Shah, Priyajeet S Parekh, Rajanikant Patel, Mehul R Chorawala

{"title":"Therapeutic potential of stem cells in colorectal cancer management: Current trends and future prospects.","authors":"Manya D Desai, Namrata B Parmar, Isha T Shah, Priyajeet S Parekh, Rajanikant Patel, Mehul R Chorawala","doi":"10.1002/dvdy.70042","DOIUrl":"https://doi.org/10.1002/dvdy.70042","url":null,"abstract":"Colorectal cancer (CRC) ranks among the leading causes of cancer-related morbidity and mortality worldwide. Despite progress in understanding its molecular intricacies, the management of CRC, especially in advanced stages, remains a significant clinical hurdle. This review delves into the evolving landscape of stem cell-based therapeutic strategies in CRC, with a specific focus on the interplay between cancer stem cells (CSCs) and CRC pathogenesis and treatment resistance. Highlighting the pivotal roles of CSCs in tumor initiation, progression, metastasis, and recurrence, the review comprehensively examines their involvement in CRC, ranging from normal colonic tissue to cancer initiation. The potential of stem cells for medicinal purposes in CRC management is explored, encompassing diverse modalities such as transplantation, differentiation therapy, immunotherapy, and gene/cell-based approaches. Challenges and opportunities associated with these strategies are also evaluated, providing insights into their clinical potential and limitations. The review also appraises preclinical investigations contributing to the understanding of CRC and stem cells. Current clinical trials, patient stratification strategies, and regulatory considerations related to stem cell-based therapies in CRC are scrutinized. Furthermore, the review explores emerging trends and future directions, including developments in stem cell technologies and ethical considerations. It highlights the transformative potential of stem cell-based therapeutic strategies in CRC.","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143997103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamics of postnatal bone development and epiphyseal synostosis in the caprine autopod. 羊自足动物出生后骨发育和骨骺结膜的动态变化。

IF 2 3区生物学

Developmental Dynamics Pub Date : 2025-05-13 DOI: 10.1002/dvdy.70038

Christopher J Panebianco, Maha Essaidi, Elijah Barnes, Ashley Williams, Karin Vancíková, Margot C Labberté, Pieter Brama, Niamh C Nowlan, Joel D Boerckel

{"title":"Dynamics of postnatal bone development and epiphyseal synostosis in the caprine autopod.","authors":"Christopher J Panebianco, Maha Essaidi, Elijah Barnes, Ashley Williams, Karin Vancíková, Margot C Labberté, Pieter Brama, Niamh C Nowlan, Joel D Boerckel","doi":"10.1002/dvdy.70038","DOIUrl":"10.1002/dvdy.70038","url":null,"abstract":"Background: Bones develop to structurally balance strength and mobility. Bone developmental dynamics are influenced by whether an animal is ambulatory at birth. Precocial species, which are ambulatory at birth, develop advanced skeletal maturity in utero and experience postnatal development under mechanical loading. Here, we characterized postnatal bone development in the lower forelimbs of precocial goats using microcomputed tomography and histology. Our analysis focused on the two phalanges 1 (P1) bones and the partially fused metacarpal bone of the goat autopod from birth through adulthood.Results: P1 cortical bone densified rapidly after birth, but cortical thickness increased continually through adulthood. Upon normalization by body mass, the P1 normalized polar moment of inertia was constant over time, suggestive of changes correlating with ambulatory loading. P1 trabecular bone increased in trabecular number and thickness until sexual maturity (12 months), while metacarpal trabeculae grew primarily through trabecular thickening. Unlike prenatal synostosis (i.e., bone fusion) of the metacarpal diaphysis, synostosis of the epiphyses occurred postnatally, prior to growth plate closure, through a unique fibrocartilaginous endochondral ossification.Conclusions: These findings implicate ambulatory loading in postnatal bone development of precocial goats and identify a novel postnatal synostosis event in the caprine metacarpal epiphysis.","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143987352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0