Developmental Dynamics最新文献_第3页

Anatomy connected 2025: American Association for Anatomy's annual meeting scientific research abstracts 解剖学连接2025：美国解剖学协会年会科学研究摘要

IF 1.5 3区生物学

Developmental Dynamics Pub Date : 2025-07-17 DOI: 10.1002/dvdy.70058

引用次数: 0

Identification of neural crest and melanoma cancer cell invasion and migration genes using high-throughput screening and deep attention networks. 利用高通量筛选和深度关注网络识别神经嵴和黑色素瘤癌细胞的侵袭和迁移基因。

IF 2 3区生物学

Developmental Dynamics Pub Date : 2025-07-10 DOI: 10.1002/dvdy.70059

J C Kasemeier-Kulesa, S Martina Perez, R E Baker, P M Kulesa

{"title":"Identification of neural crest and melanoma cancer cell invasion and migration genes using high-throughput screening and deep attention networks.","authors":"J C Kasemeier-Kulesa, S Martina Perez, R E Baker, P M Kulesa","doi":"10.1002/dvdy.70059","DOIUrl":"10.1002/dvdy.70059","url":null,"abstract":"Background: Cell migration and invasion are well-coordinated in development and disease but remain poorly understood. We previously showed that the neural crest (NC) cell migratory wavefront shares a 45-gene panel with other cell invasion phenomena. To rapidly and systematically identify critical genes, we performed a high-throughput siRNA screen and statistical and deep learning analyses to determine changes in NC- versus non-NC-derived human cell line behaviors.Results: We find 14 out of 45 genes significantly reduced c8161 melanoma cell migration; four of the 14 genes altered leader cell motility (BMP4, ITGB1, KCNE3, and RASGRP1). Deep learning identified marked disruptions in cell-neighbor interactions after BMP4 or RASGRP1 knockdown in c8161 cells. Recombinant proteins added to the culture media revealed five out of the 11 known secreted molecules stimulated c8161 cell migration. BMP4 knockdown severely reduced c8161 in vivo invasion in a chick embryo transplant model. Addition of BMP4 protein to the culture media of BMP4-siRNA-treated c8161 cells rescued cell migratory ability.Conclusion: High-throughput screening and deep learning distilled a 45-gene panel to a small subset of genes critical to melanoma and warrant deeper in vivo functional analysis for their role and potential synergies in driving NC cell migration and invasion.","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144599659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Robinow syndrome DVL1 variants disrupt morphogenesis and appendage formation in a Drosophila disease model. Robinow综合征DVL1变异在果蝇疾病模型中破坏形态发生和附属物形成。

IF 2 3区生物学

Developmental Dynamics Pub Date : 2025-07-02 DOI: 10.1002/dvdy.70056

Gamze Akarsu, Katja R MacCharles, Kenneth Kin Lam Wong, Joy M Richman, Esther M Verheyen

{"title":"Robinow syndrome DVL1 variants disrupt morphogenesis and appendage formation in a Drosophila disease model.","authors":"Gamze Akarsu, Katja R MacCharles, Kenneth Kin Lam Wong, Joy M Richman, Esther M Verheyen","doi":"10.1002/dvdy.70056","DOIUrl":"https://doi.org/10.1002/dvdy.70056","url":null,"abstract":"Background: Robinow syndrome is a rare developmental syndrome caused by variants in genes in Wnt signaling pathways. We previously showed that expression of patient variants in Dishevelled 1 (DVL1) in Drosophila and chicken models disrupts the balance of canonical and non-canonical Wnt signaling.Results: In this study, we further examine morphological changes that occur due to expression of DVL11519ΔT, which serves as a prototype for other pathogenic variants. We show that epithelial imaginal disc development is disrupted in legs and wings and accompanied by increased cell death, without changes in cell proliferation. By inhibiting caspase-dependent cell death, we show that the altered epithelial morphology is not solely due to variant-induced cell death. Furthermore, we find alterations of basement membrane components and modulators. Notably we find ectopic Mmp1 expression and tissue distortion, which is dependent on JNK signaling. We also find an abnormal abundance of Drosophila collagen IV (Viking) in pupal wing development. Due to the complex nature of appendage development, we also examined the Bone Morphogenetic Protein pathway and found elevated signaling activity via the transcriptional readout dad-lacZ.Conclusions: Through these studies, we have gained more insight into the developmental consequences of DVL1 variants implicated in autosomal dominant Robinow syndrome.","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144539512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Endoderm differentiates into a transient epidermis in the mouse perineum. 小鼠会阴内胚层分化为短暂表皮。

IF 2 3区生物学

Developmental Dynamics Pub Date : 2025-06-25 DOI: 10.1002/dvdy.70050

Christine E Larkins, Daniel M Grunberg, Gabriel M Daniels, Erik J Feldtmann, Martin J Cohn

{"title":"Endoderm differentiates into a transient epidermis in the mouse perineum.","authors":"Christine E Larkins, Daniel M Grunberg, Gabriel M Daniels, Erik J Feldtmann, Martin J Cohn","doi":"10.1002/dvdy.70050","DOIUrl":"10.1002/dvdy.70050","url":null,"abstract":"Background: In eutherian mammals, the embryonic cloaca is partitioned into genitourinary and anorectal canals by the urorectal septum. In the mouse embryo, the urorectal septum contributes to the perineum, which separates the anus from the external genitalia. During the growth of the urorectal septum, endodermal epithelium of the cloaca is displaced to the surface of the perineum, where endodermal cells are integrated into the developing skin. However, it is unknown whether the endodermal lineage of the perineum acquires true epidermal identity, an enigmatic fate for endodermal cells.Results: We find that endodermal cells that reach the surface of the perineum express markers of basal, spinous, and granular epidermis. During postnatal development, the endodermal lineage of the perineum epidermis undergoes terminal differentiation and desquamation and is replaced by adjacent ectoderm. Live imaging and single-cell tracking show that ectodermal cells move at a faster velocity in a lateral-to-medial direction, indicating convergence toward the narrow band of endoderm that lies between the anus and external genitalia.Conclusions: Cloacal endoderm differentiates into a non-renewing, transient epidermis at the midline of the perineum. Differences in directionality and velocity of cell movement patterns between endodermal and ectodermal cells suggest that the perineum epidermis develops by convergent extension.","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144483555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combinatorial expression of glial transcription factors induces Schwann cell-specific gene expression in mouse embryonic fibroblasts. 神经胶质转录因子组合表达诱导小鼠胚胎成纤维细胞雪旺细胞特异性基因表达。

IF 2 3区生物学

Developmental Dynamics Pub Date : 2025-06-19 DOI: 10.1002/dvdy.70054

Lauren Belfiore, Anjali Balakrishnan, Yacine Touahri, Dawn Zinyk, Humna Noman, Satoshi Okawa, Jeff Biernaskie, Carol Schuurmans

{"title":"Combinatorial expression of glial transcription factors induces Schwann cell-specific gene expression in mouse embryonic fibroblasts.","authors":"Lauren Belfiore, Anjali Balakrishnan, Yacine Touahri, Dawn Zinyk, Humna Noman, Satoshi Okawa, Jeff Biernaskie, Carol Schuurmans","doi":"10.1002/dvdy.70054","DOIUrl":"10.1002/dvdy.70054","url":null,"abstract":"Background: Schwann cells provide peripheral nerve trophic support, myelinate axons, and assist in repair. However, Schwann cell repair capacity is limited by chronic injury, disease, and aging. Schwann cell reprogramming is a cellular conversion strategy that could provide a renewable cell supply to repair injured nerves. Here, we developed a plasmid-based approach to test the Schwann cell conversion potential of four glial transcription factors.Results: We employed four transcription factors implicated in Schwann cell differentiation and repair: Sox10, Sox2, Jun, and Pax3. Expression vectors were generated for Sox10 alone and two triple transcription factor combinations: Jun-Pax3-Sox2 (triple 1, T1) and Sox10-Jun-Sox2 (triple 2, T2). Mouse embryonic fibroblasts (MEFs) were transfected with these vectors, transferred to glial inductive media, and Schwann cell-marker expression was in assessed by immunostaining, flow cytometry, and qPCR. All expression vectors repressed fibroblast-specific gene expression. However, T2 was most efficient at generating O4+ Schwann cell-like cells, which had some capacity to myelinate denervated axons from explanted dorsal root ganglia. In comparison, T1 more efficiently induced repair Schwann cell-marker expression in converted O4+ cells.Conclusions: T1 and T2 convert MEFs to Schwann cells with different efficacies and gene expression profiles, and may provide cell-based therapies for peripheral nerve repair.","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144332577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic evolution of EGF-CFC genes in deuterostomes 后口动物EGF-CFC基因的基因组进化。

IF 1.5 3区生物学

Developmental Dynamics Pub Date : 2025-06-19 DOI: 10.1002/dvdy.70051

Natalia A. Shylo, Paul A. Trainor

{"title":"Genomic evolution of EGF-CFC genes in deuterostomes","authors":"Natalia A. Shylo, Paul A. Trainor","doi":"10.1002/dvdy.70051","DOIUrl":"10.1002/dvdy.70051","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 EGF-CFC proteins are a bilaterian innovation, but they are best known for their roles in Nodal signaling during gastrulation and left–right patterning in vertebrates. Species with multiple family members show evidence of functional specialization. For example, in mouse, Cripto is required for gastrulation, whereas CFC1 is involved in left–right patterning. However, members of the EGF-CFC family across model organisms exhibit limited sequence conservation beyond the EGF-CFC domain, posing challenges for determining their evolutionary history and functional conservation.\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 In this study, we describe the evolutionary history of the EGF-CFC family of proteins across several branches of deuterostomes, with a particular focus on vertebrates. We trace the EGF-CFC gene family from a single gene in the deuterostome ancestor through its expansion and functional specialization in tetrapods, and subsequent gene loss and translocation in eutherian mammals. Mouse Cripto and CFC1, zebrafish Tdgf1, and each Xenopus EGF-CFC gene (Tdgf1, Tdgf1.2 and Cripto.3) are all descendants of the ancestral deuterostome Tdgf1 gene.\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 We propose that subsequent to EGF-CFC family expansion in tetrapods, Tdgf1B (Xenopus Tdgf1.2) acquired specialization in the left–right patterning cascade, and then after its translocation in eutherians to a different chromosomal location, CFC1 has maintained that specialization.\u0000 </section>\u0000 </div>","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":"254 8","pages":"935-949"},"PeriodicalIF":1.5,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144324707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dental development in the tropical gar (Atractosteus tropicus) and the evolution of vertebrate dentitions. 热带黄鳝（Atractosteus tropicus）牙齿的发育和脊椎动物牙齿的进化。

IF 2 3区生物学

Developmental Dynamics Pub Date : 2025-06-19 DOI: 10.1002/dvdy.70055

Anna Pospisilova, Jan Stundl, Lenin Arias-Rodriguez, Robert Cerny, Vladimír Soukup

{"title":"Dental development in the tropical gar (Atractosteus tropicus) and the evolution of vertebrate dentitions.","authors":"Anna Pospisilova, Jan Stundl, Lenin Arias-Rodriguez, Robert Cerny, Vladimír Soukup","doi":"10.1002/dvdy.70055","DOIUrl":"10.1002/dvdy.70055","url":null,"abstract":"Background: Dentitions have diversified enormously during vertebrate evolution, involving reductions, modifications, or allocations to prey seizing and processing regions. A combination of ancient and novel features related to dental and oropharyngeal apparatuses is found in extant lineages of non-teleost fishes, such as the gars. While relevant to evolutionary-developmental studies, gars have largely been overlooked regarding how their dentition arises, thus leaving our comprehension of the evolutionary history of vertebrate dentitions incomplete.Results: Here, we complement this knowledge gap by studying dental development in the tropical gar, Atractosteus tropicus. We follow ontogenetic changes from the initiation, tooth germ addition to the establishment of the larval replacing dentition. We pay attention to the progressive appearance of tooth fields, the emergence of dental patterns, the development of folded dentin morphology, and features related to tooth resorption and replacement. Furthermore, we identify snout elongation as the critical period when the general dentition layout becomes established.Conclusions: Our study depicts the gar oropharyngeal apparatus as a system that is established based on patterned initiation, differential growth, replacement, and complex shaping of teeth. These features form a reference standpoint for the likely developmental processes employed in dentitions of fossil stem and crown bony vertebrates, including ray-finned fishes and tetrapods.","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144332578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Abstract 摘要。

IF 1.5 3区生物学

Developmental Dynamics Pub Date : 2025-06-17 DOI: 10.1002/dvdy.70043

引用次数: 0

Differential sensitivity of midline development to mitosis during and after primitive streak extension. 原始条纹延伸期间和之后中线发育对有丝分裂的不同敏感性。

IF 1.5 3区生物学

Developmental Dynamics Pub Date : 2025-06-12 DOI: 10.1002/dvdy.70045

Zhiling Zhao, Rieko Asai, Takashi Mikawa

{"title":"Differential sensitivity of midline development to mitosis during and after primitive streak extension.","authors":"Zhiling Zhao, Rieko Asai, Takashi Mikawa","doi":"10.1002/dvdy.70045","DOIUrl":"10.1002/dvdy.70045","url":null,"abstract":"Background: Midline establishment is a fundamental process during early embryogenesis for Bilaterians. Midline morphogenesis in non-amniotes can occur without mitosis, through Planar Cell Polarity (PCP) signaling. By contrast, amniotes utilize both cellular processes for developing the early midline landmark, the primitive streak (PS). This study focused on the role of cell proliferation for midline development at pre- and post-PS-extension stages and analyzed PCP signaling components at post-PS-extension stages.Results: In contrast to pre-PS-extension stages, embryos under mitotic arrest during the post-PS-extension preserved notochord (NC) extension and Hensen's node (HN)/PS regression judged by both morphology and marker genes; although they became shorter, their lengths remained proportional to the embryo length. Laterality and segmentation of paraxial mesoderm were lost upon mitotic arrest. Accompanied by mitotic arrest-induced embryonic size reduction, cells including midline tissue displayed hypertrophy.Conclusion: This study has identified at least two distinct mitosis sensitivity phases during early midline development: One is PS extension that requires both mitosis and PCP, and the other is mitotic arrest-resistant midline development at post-PS-extension stages, with a still undefined influence by PCP signaling components.","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12354007/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144274442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ontogeny of RSPO1, FOXL2, and RUNX1 during ovarian differentiation in the marsupial tammar wallaby. 有袋袋鼠卵巢分化过程中RSPO1、FOXL2和RUNX1的个体发育。

IF 2 3区生物学

Developmental Dynamics Pub Date : 2025-06-07 DOI: 10.1002/dvdy.70048

Monika R Paranjpe, Yu Chen, Geoff Shaw, Marilyn B Renfree

{"title":"Ontogeny of RSPO1, FOXL2, and RUNX1 during ovarian differentiation in the marsupial tammar wallaby.","authors":"Monika R Paranjpe, Yu Chen, Geoff Shaw, Marilyn B Renfree","doi":"10.1002/dvdy.70048","DOIUrl":"https://doi.org/10.1002/dvdy.70048","url":null,"abstract":"Background: RSPO1 and FOXL2 are female sex-determining genes involved in the differentiation and organization of the ovary in some eutherian mammals. Mutations or loss of function of these genes are associated with partial to full sex reversal in mice, humans, and goats. RUNX1 may also play a role in ovarian development, but its expression in marsupials has not been examined as yet. We studied the conservation and protein localization of RSPO1, FOXL2, and RUNX1 orthologs in the marsupial tammar wallaby (Notamacropus eugenii) compared to other vertebrates.Results: RSPO1, FOXL2, and RUNX1 were highly conserved in their sequences across all vertebrates examined. The localization of these proteins in the tammar ovary was studied from Day 18 postpartum to adulthood. RSPO1, FOXL2, and RUNX1 were expressed in the granulosa cells of the early ovary and granulosa cells of the mature ovary, while RSPO1 expression was also found in the intra-ovarian rete and cell membrane of germ cells during the period of germ cell meiosis and meiotic arrest.Conclusions: RSPO1, FOXL2, and RUNX1 are highly conserved in the vertebrate ovary-determining pathway and were expressed in the tammar wallaby in a manner consistent with their role in the ovarian differentiation of eutherian mammals.","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144246970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0