Developmental Dynamics最新文献

{"title":"Rodent monocyte-derived macrophages do not express CD163: Comparative analysis using macrophages from living boreoeutherians.","authors":"Yoichi Saito, Yukio Fujiwara, Yasuka L Yamaguchi, Satomi S Tanaka, Kyoko Miura, Yoshiyuki Hizukuri, Kyoko Yamashiro, Yasuhiro Hayashi, Yuta Nakashima, Yoshihiro Komohara","doi":"10.1002/dvdy.70036","DOIUrl":"https://doi.org/10.1002/dvdy.70036","url":null,"abstract":"<p><strong>Background: </strong>CD163 is a scavenger receptor predominantly expressed on the surfaces of macrophages in various mammalian species and is a marker of anti-inflammatory (M2-like) macrophages. High density of CD163-positive tumor-associated macrophages (TAMs) is associated with worse prognosis in various patient tumors. Interestingly, studies on mice have shown that CD163-positive TAMs only infiltrate the margins of tumor tissues, not the center. Based on these observations, we hypothesized that circulating monocyte-derived macrophages (MDMs), which are the origin of most TAMs, do not express CD163 in mice.</p><p><strong>Results: </strong>We examined CD163 expression in MDMs, differentiated from healthy animals in vitro, and in normal, pathogenic, and tumorigenic macrophages infiltrating various tumors and organs across multiple species including primates, rodents, cetartiodactylans, and carnivores. We found that MDMs, including TAMs, do not express CD163 in mice. Our findings also suggest that murine CD163-positive macrophages likely originate from a specific subset of resident macrophages, namely fetal liver monocytes/macrophages, as indicated by fetal analysis. Furthermore, we revealed that the CD163-negative expression pattern in MDMs is a trait shared by the rodent clade.</p><p><strong>Conclusions: </strong>Rodent MDMs do not express CD163, a phenotype not shared with MDMs of other mammals. Our findings caution against the extrapolation of rodent experimental results to other animal models.</p>","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143985069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial highlights","authors":"Paul A. Trainor","doi":"10.1002/dvdy.70030","DOIUrl":"https://doi.org/10.1002/dvdy.70030","url":null,"abstract":"&lt;p&gt;Every organism is a model organism for understanding development, evolution, disease, and regeneration, and we have only begun to scratch the surface of the interdisciplinary genetic, molecular, cellular, and developmental mechanisms that regulate these biological processes. These “Highlights” denote exciting advances recently reported in &lt;i&gt;Developmental Dynamics&lt;/i&gt; that illustrate the complex dynamics of developmental biology.&lt;/p&gt;&lt;p&gt;“Stage-by-stage exploration of normal embryonic development in the Arabian killifish, &lt;i&gt;Aphanius dispar&lt;/i&gt;” by Amena Alsakran, Rashid Minhas, Atyaf Hamied, Rod Wilson, Mark Ramsdale, and Tetsuhiro Kudoh Fan, &lt;i&gt;DevDyn&lt;/i&gt; 254.5, pp. 380–395, https://doi.org/10.1002/dvdy.738.&lt;/p&gt;&lt;p&gt;Teleost fish such as zebrafish and medaka are attractive models for studying developmental processes due to their small size and transparency. In addition, zebrafish and medaka have classically been used to model genetic diseases and infection. However, it is still desirable to develop new fish models that can facilitate the investigation of environmental changes and pollution in estuarine, marine, and high-salinity environments. The Arabian killifish (&lt;i&gt;Aphanius dispar&lt;/i&gt;) is a small teleost fish in which the embryo and chorion are very transparent, which facilitates high resolution and high throughput imaging. &lt;i&gt;A. dispar&lt;/i&gt; has been used as an effective biological control agent for mosquito larvae. This study investigated the step-by-step development of &lt;i&gt;A. dispar&lt;/i&gt; embryos, delineating key developmental milestones from the maternal to hatching stages, and demonstrated that temperature has a significant effect on embryonic development, with accelerated development at higher temperatures. &lt;i&gt;A. dispar&lt;/i&gt; exhibits broad thermal tolerance and extended independent feeding capabilities, making it a promising model organism for ecotoxicology and disease pathogenesis studies.&lt;/p&gt;&lt;p&gt;“Effects of life history strategies and habitats on limb regeneration in plethodontid salamanders” by Vivien Bothe, Hendrik Müller, Neil Shubin, and Nadia Fröbisch Fan, &lt;i&gt;DevDyn&lt;/i&gt; 254.5, pp. 396–419, https://doi.org/10.1002/dvdy.742.&lt;/p&gt;&lt;p&gt;Salamanders are renowned as the only tetrapod capable of fully regenerating its limbs. Indeed, in addition to their limbs and tails, salamanders are the only tetrapod able to regenerate various other anatomical structures, including organs such as the lens, heart, and liver, repeatedly and throughout their entire life. However, salamanders are a highly diverse clade of tetrapods with around 850 currently known species, and yet research on tetrapod regeneration has largely been based on a small number of salamander species, primarily the Mexican axolotl &lt;i&gt;Ambystoma mexicanum&lt;/i&gt; and, to a lesser extent, &lt;i&gt;Pleurodeles waltl&lt;/i&gt; and &lt;i&gt;Notophthalmus viridescens&lt;/i&gt;. Interestingly, some studies have suggested that certain salamanders may not be able to regenerate their limbs at all. Therefore, it remains to be dete","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":"254 5","pages":"378-379"},"PeriodicalIF":2.0,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/dvdy.70030","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143900906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tissue-specific requirement of Polr1D in the prothoracic gland for ecdysone-mediated developmental transitions in Drosophila melanogaster.","authors":"Bridget M Walker, Ryan J Palumbo, Bruce A Knutson","doi":"10.1002/dvdy.70029","DOIUrl":"https://doi.org/10.1002/dvdy.70029","url":null,"abstract":"<p><strong>Background: </strong>POLR1D is a shared subunit of RNA Polymerases I and III, which transcribe the rRNA incorporated into ribosomes. Mutations in POLR1D cause Treacher Collins syndrome, a craniofacial disorder that arises from impaired ribosome biogenesis in neural crest cells. Previously, we found that RNAi knockdown of Polr1D in several non-neural Drosophila tissues caused developmental defects that phenocopy mutations affecting ecdysone signaling. Ecdysone is a steroid hormone produced in the prothoracic gland (PG) of insects that triggers developmental transitions. Here, we show that Polr1D is required for PG development and ecdysone production to facilitate larval developmental transitions.</p><p><strong>Results: </strong>We found that Polr1D RNAi in the PG causes larval developmental arrest due to defective peripheral ecdysone signaling. We also found that Polr1D is required for the growth of PG cells and for maintaining nucleolar structure. We found that Polr1D is required for the synthesis of mature ribosomes and the production of the Pol III-transcribed 7SK RNA. Furthermore, developmental arrest of Polr1D RNAi larvae and Polr1D mutant (G30R) larvae was partially rescued by treatment with exogenous ecdysone.</p><p><strong>Conclusion: </strong>These results demonstrate a role for Drosophila Polr1D in PG development and suggest that disruptions in human Polr1D might impact additional cell types during development.</p>","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143995696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Myomaker and Myomixer are required for craniofacial myoblast fusion in zebrafish.","authors":"Zhanxiong Zhang, Pengzheng Yong, Qiaomu Hu, Shaojun Du","doi":"10.1002/dvdy.70031","DOIUrl":"https://doi.org/10.1002/dvdy.70031","url":null,"abstract":"<p><strong>Background: </strong>Craniofacial and trunk skeletal muscles are derived from different progenitor populations during development. Trunk skeletal muscles contain mostly multinucleated myofibers that are formed through myoblast fusion. However, myoblast fusion in craniofacial muscles and its molecular regulation are not well understood. Recent studies revealed that genetic mutations in MYOMAKER and MYOMIXER fusogens in humans cause Carey-Fineman-Ziter Syndrome (CFZS), characterized by facial weakness and lower jaw deformity.</p><p><strong>Results: </strong>Previous studies in zebrafish revealed that knockout of myomaker and myomixer resulted in deformed craniofacial formation. To establish the causal connection between loss of fusogen function and craniofacial deformities, we characterized myoblast fusion in zebrafish craniofacial muscles. Our results demonstrate that myomaker and myomixer are expressed in both slow and fast craniofacial muscles, and loss of these fusogens results in defects in craniofacial myoblast fusion. Interestingly, unlike trunk muscles of early embryos and larvae that show fast-fiber-specific fusogen expression and fusion while slow muscle fusion only occurs at 3 weeks post-fertilization, both slow and fast craniofacial muscles fuse as early as 3 days post-fertilization.</p><p><strong>Conclusions: </strong>Collectively, this study demonstrates that myomaker and myomixer are expressed in both slow and fast-twitch craniofacial muscles and are essential for myoblast fusion and the development of craniofacial muscles.</p>","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143988809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pre-oviposition development of the brown anole (Anolis sagrei).","authors":"Antonia Weberling, Natalia A Shylo, Bonnie K Kircher, Hannah Wilson, Melainia McClain, Marta Marchini, Katherine B Starr, Thomas J Sanger, Florian Hollfelder, Paul A Trainor","doi":"10.1002/dvdy.70027","DOIUrl":"https://doi.org/10.1002/dvdy.70027","url":null,"abstract":"<p><strong>Background: </strong>The brown anole, Anolis sagrei, has emerged as a representative squamate species for developmental studies during the past decades. Novel functional tools have been established to manipulate embryogenesis through genome editing or the introduction of small molecule inhibitors, and their effective use requires a thorough understanding of early anole embryogenesis. To enable precise and reproducible staging of anole embryos, we need knowledge of the progression of anole embryogenesis and morphogenesis. While post-oviposition development has been described, the pre-oviposition period remains to be explored.</p><p><strong>Results: </strong>We provide the first staging series of pre-oviposition development for the brown anole. Analyzing the follicles and embryos through brightfield imaging, SEM, STEM, histology, and DAPI staining, we define 26 distinct developmental stages. Furthermore, we followed heart development, neural crest cell migration, and central nervous system development using immunofluorescence analyses and provide new comparative insights into the morphogenesis of each of these organ systems.</p><p><strong>Conclusions: </strong>Our dataset reveals that peri-gastrulation morphogenesis up to the initiation of neurulation diverges significantly from chick, the common representative model of reptile embryogenesis. With this study, we establish the brown anole as a squamate model organism for cross-clade evolutionary studies of early embryogenesis.</p>","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143977491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamic expression of lamin B1 during adult neurogenesis in the vertebrate brain.","authors":"Diana Zhilina, Lizbeth A Bolaños Castro, Juan Sebastian Eguiguren, Sara Zocher, Anne Karasinsky, Dimitri Widmer, Alexandre Espinós, Victor Borrell, Michael Brand, Kyoko Miura, Oliver Zierau, Maximina H Yun, Tomohisa Toda","doi":"10.1002/dvdy.70023","DOIUrl":"https://doi.org/10.1002/dvdy.70023","url":null,"abstract":"<p><strong>Background: </strong>In mammals, specific brain regions such as the dentate gyrus (DG) of the hippocampus and the subventricular zone (SVZ) of the lateral ventricles harbor adult neural stem/progenitor cells (ANSPCs) that give rise to new neurons and contribute to structural and functional brain plasticity. In contrast, other vertebrates such as salamanders and zebrafish exhibit a widely distributed neurogenic niches throughout the brain, suggesting a greater neurogenic capacity in adulthood. However, the mechanisms underlying this divergence in neurogenic potential among vertebrates remain elusive. To address this, we examined the expression dynamics of a critical epigenetic regulator for the long-term maintenance of murine ANSPCs, lamin B1, during adult neurogenesis across the vertebrate spectrum.</p><p><strong>Results: </strong>Lamin B1 expression patterns during adult neurogenesis are conserved among mammals including mouse, naked mole-rat, and ferret. However, these patterns differ between mammals and anamniotes. In mammals, neural stem cells and neuroblasts exhibited higher lamin B1 levels, and differentiated neurons possessed lower lamin B1 levels. On the other hand, anamniotes showed the opposite patterns of lamin B1 expression, with higher levels in neurons compared to stem cells.</p><p><strong>Conclusions: </strong>Our study shows that the lamin B1 expression pattern during adult neurogenesis differs between species, and that changes in lamin B1 protein sequence may contribute to the differences in lamin B1 expression patterns. This study highlights potential differences in cell-autonomous epigenetic regulation in the maintenance of ANSPC pools in the adult brain among species.</p>","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143991519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial highlights","authors":"Paul A. Trainor","doi":"10.1002/dvdy.70024","DOIUrl":"https://doi.org/10.1002/dvdy.70024","url":null,"abstract":"&lt;p&gt;Every organism is a model organism for understanding development, evolution, disease, and regeneration, and we have only begun to scratch the surface of the interdisciplinary genetic, molecular, cellular, and developmental mechanisms that regulate these biological processes. These “Highlights” denote exciting advances recently reported in &lt;i&gt;Developmental Dynamics&lt;/i&gt; that illustrate the complex dynamics of developmental biology.&lt;/p&gt;&lt;p&gt;“Review on pathogenesis and treatment of Alzheimer's disease” by Jinxia Cai, Yanqing Liu, and Haojun Fan. &lt;i&gt;DevDyn&lt;/i&gt; 254.4, pp. 296–309. https://doi.org/10.1002/dvdy.762&lt;/p&gt;&lt;p&gt;Alzheimer's disease is a progressive neurodegenerative disorder, characterized primarily by memory and visuospatial skills impairment, personality or behavioral changes, and executive dysfunction. Alzheimer's disease, which has been speculated to begin in an individual up to 20 years before the onset of any symptoms, is increasing in prevalence and incidence. Age, genetics, environment, lifestyle habits, emotions, education, disease, and race are all considered to be causative factors in the etiology and pathogenesis of Alzheimer's disease, but despite extensive research and our improved understanding of β-amyloid aggregation, hyperphosphorylated tau, and neuroinflammation in its pathogenesis, there are currently no effective treatments to ameliorate or prevent neurodegeneration. This review article provides a timely and comprehensive analysis of the underlying etiology and pathogenesis of Alzheimer's disease, as well as an overview of potential Alzheimer's disease treatments.&lt;/p&gt;&lt;p&gt;“ARHGAP29 promotes keratinocyte proliferation and migration in vitro and is dispensable for in vivo wound healing” by Lindsey Rhea, Tanner Reeb, Emily Adelizzi, Bailey Garnica, Allison Stein, Alexis Kollash, Elliot Dunnwald, and Martine Dunnwald. &lt;i&gt;DevDyn&lt;/i&gt; 254.4, pp. 310–329. https://doi.org/10.1002/dvdy.759&lt;/p&gt;&lt;p&gt;Skin, which is the body's largest organ, performs many functions. It helps to regulate temperature, prevent water loss, enable tactile sensation, and serve as a protective structural barrier to external environments. Composed of epidermis, dermis, and hypodermis, perturbations of skin development and homeostasis are associated with disease and defective wound healing. Proper maintenance of epithelial integrity requires the coordinated proliferation and migration of keratinocytes, which can rapidly respond to changes in the extracellular environment. The Rho family of GTPases, including Rac, Cdc42, and Rho, is a key regulator of cell morphology, migration, and wound healing. The authors previously showed that interferon regulatory factor 6 (IRF6) promotes keratinocyte migration via RhoA and is required for proper wound healing, especially in the surgical repair of orofacial clefts. In this study, the authors propose that ARHGAP29, which is a RhoGTPase that preferentially regulates RhoA, is a downstream effector of IRF6 and plays an important rol","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":"254 4","pages":"294-295"},"PeriodicalIF":2.0,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/dvdy.70024","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143801698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive observation of histone lysine lactylation during gametogenesis of Drosophila melanogaster.","authors":"Yoshiki Hayashi, Ban Sato, Rio Kageyama, Kenji Miyado, Daisuke Saito, Satoru Kobayashi, Natsuko Kawano","doi":"10.1002/dvdy.70010","DOIUrl":"https://doi.org/10.1002/dvdy.70010","url":null,"abstract":"<p><strong>Background: </strong>Histone post-translational modification (PTM) is an important epigenomic regulation content and an essential process regulating gene expression. Histone lysine lactylation is the newly identified histone PTM that utilizes the lactyl moiety for its modification. Although histone lysine lactylation is considered an essential outcome of the Wardburg effects and the interconnection between cellular metabolism and gene regulation, the developmental contexts involving this PTM are largely unknown. In this study, we comprehensively observed histone lysine lactylation during Drosophila oogenesis, one of the developmental contexts in which chromatin regulation plays crucial roles.</p><p><strong>Results: </strong>Our study revealed that lactylation on the specific histone lysine mainly occurs in the oocyte karyosome and condensed meiotic chromosome, suggesting histone lysine lactylation has a vital role in female meiosis. Interestingly, one of the histone lysine lactylations, lactylation of lysine 14 of histone H3, is intensively observed in the meiotic germline in the mouse ovary, suggesting that lactylation has an evolutionarily conserved role.</p><p><strong>Conclusions: </strong>Our results revealed that histone lysine lactylation is predominantly present in transcriptionally repressive meiotic chromatin, which contradicts the previously reported function of histone lactylation in transcriptional activation. This study, therefore, provides the first fundamental information to understand the role of histone lysine lactylation in the germline and repressive chromatin.</p>","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143729298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gfi1 in the inner ear: A retrospective review.","authors":"Zhuo Li, Hongzhi Chen, Hao Feng","doi":"10.1002/dvdy.70019","DOIUrl":"https://doi.org/10.1002/dvdy.70019","url":null,"abstract":"<p><p>Gfi1 plays an important role in the development of hair cells (HCs), as indicated by its ability to regulate the expression of HC-related genes while the organ of Corti is developing. Given that the HCs and the supporting cells (SCs) are coming from a common stem/progenitor cell pool, it is conceivable to regenerate HCs from SCs that ectopically express Gfi1. The focus of this review was to elucidate the role of Gfi1 in controlling the development of HCs by dissecting the phenotypes of the inner ear in Gfi1-mutated mouse lines. In addition, we reviewed studies of regeneration in the mammalian inner ear, by which we discussed the novel function of Gfi1 as an essential factor in guiding non-HCs toward an HC destiny in coordination with Atoh1 and Pou4f3. Finally, we summarized the known Gfi1-specific Cre/CreER/reporter mouse lines and highlighted the pros and cons of each line, with the aim of providing insights for use in future studies. In summary, a better understanding of Gfi1 and its diverse roles is beneficial for advancing studies of HC regeneration in the inner ear.</p>","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lineage labeling with zebrafish hand2 Cre and CreERT2 recombinase CRISPR knock-ins.","authors":"Zhitao Ming, Fang Liu, Hannah R Moran, Robert L Lalonde, Megan Adams, Nicole K Restrepo, Parnal Joshi, Stephen C Ekker, Karl J Clark, Iddo Friedberg, Saulius Sumanas, Chunyue Yin, Christian Mosimann, Jeffrey J Essner, Maura McGrail","doi":"10.1002/dvdy.70022","DOIUrl":"10.1002/dvdy.70022","url":null,"abstract":"<p><strong>Background: </strong>The ability to generate endogenous Cre recombinase drivers using CRISPR-Cas9 knock-in technology allows lineage tracing, cell type-specific gene studies, and in vivo validation of inferred developmental trajectories from phenotypic and gene expression analyses. This report describes endogenous zebrafish hand2 Cre and CreERT2 drivers generated with GeneWeld CRISPR-Cas9 precision targeted integration.</p><p><strong>Results: </strong>hand2-2A-cre and hand2-2A-creERT2 knock-ins crossed with ubiquitous loxP-based Switch reporters led to broad labeling in expected mesodermal and neural crest-derived lineages in branchial arches, cardiac, fin, liver, intestine, and mesothelial tissues, as well as enteric neurons. Novel patterns of hand2 lineage tracing appeared in venous blood vessels. CreERT2 induction at 24 h reveals hand2-expressing cells in the 24- to 48-h embryo contribute to the venous and intestinal vasculature. Induction in 3 dpf larvae restricts hand2 lineage labeling to mesoderm-derived components of the branchial arches, heart, liver, and enteric neurons.</p><p><strong>Conclusions: </strong>hand2 progenitors from the lateral plate mesoderm and ectoderm contribute to numerous lineages in the developing embryo. At later stages, hand2-expressing cells are restricted to a subset of lineages in the larva. The endogenous hand2 Cre and CreERT2 drivers establish critical new tools to investigate hand2 lineages in zebrafish embryogenesis and larval organogenesis.</p>","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}