Genomic evolution of EGF-CFC genes in deuterostomes.

IF 1.5 3区 生物学 Q2 ANATOMY & MORPHOLOGY
Natalia A Shylo, Paul A Trainor
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引用次数: 0

Abstract

Background: EGF-CFC proteins are a bilaterian innovation, but they are best known for their roles in Nodal signaling during gastrulation and left-right patterning in vertebrates. Species with multiple family members show evidence of functional specialization. For example, in mouse, Cripto is required for gastrulation, whereas CFC1 is involved in left-right patterning. However, members of the EGF-CFC family across model organisms exhibit limited sequence conservation beyond the EGF-CFC domain, posing challenges for determining their evolutionary history and functional conservation.

Results: In this study, we describe the evolutionary history of the EGF-CFC family of proteins across several branches of deuterostomes, with a particular focus on vertebrates. We trace the EGF-CFC gene family from a single gene in the deuterostome ancestor through its expansion and functional specialization in tetrapods, and subsequent gene loss and translocation in eutherian mammals. Mouse Cripto and CFC1, zebrafish Tdgf1, and each Xenopus EGF-CFC gene (Tdgf1, Tdgf1.2 and Cripto.3) are all descendants of the ancestral deuterostome Tdgf1 gene.

Conclusions: We propose that subsequent to EGF-CFC family expansion in tetrapods, Tdgf1B (Xenopus Tdgf1.2) acquired specialization in the left-right patterning cascade, and then after its translocation in eutherians to a different chromosomal location, CFC1 has maintained that specialization.

后口动物EGF-CFC基因的基因组进化。
背景:EGF-CFC蛋白是一种双边创新,但它们最为人所知的作用是在脊椎动物原肠形成和左右模式的节点信号传导中发挥作用。具有多个家族成员的物种显示出功能特化的证据。例如,在小鼠中,原肠胚形成需要Cripto,而CFC1则参与左右模式。然而,模型生物中EGF-CFC家族成员在EGF-CFC结构域之外表现出有限的序列保守性,这为确定其进化史和功能保守性带来了挑战。结果:在这项研究中,我们描述了EGF-CFC家族蛋白在几个后口动物分支中的进化史,特别关注脊椎动物。我们追踪了EGF-CFC基因家族,从后口动物祖先的单个基因开始,经过其在四足动物中的扩展和功能特化,以及随后在真兽哺乳动物中的基因丢失和易位。小鼠Cripto和CFC1、斑马鱼Tdgf1和爪蟾EGF-CFC基因(Tdgf1、Tdgf1.2和Cripto.3)都是祖先后口动物Tdgf1基因的后代。结论:我们认为,随着EGF-CFC家族在四足动物中的扩展,Tdgf1B (Xenopus Tdgf1.2)在左-右模式级联中获得了特化,然后在真足动物中易位到不同的染色体位置后,CFC1保持了这种特化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Developmental Dynamics
Developmental Dynamics 生物-发育生物学
CiteScore
5.10
自引率
8.00%
发文量
116
审稿时长
3-8 weeks
期刊介绍: Developmental Dynamics, is an official publication of the American Association for Anatomy. This peer reviewed journal provides an international forum for publishing novel discoveries, using any model system, that advances our understanding of development, morphology, form and function, evolution, disease, stem cells, repair and regeneration.
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