Developmental Dynamics最新文献_第4页

Sox9 in the second heart field and the development of the outflow tract; implications for cardiac septation and valve formation. 第二心野Sox9与流出道发育；对心脏分隔和瓣膜形成的影响。

IF 2 3区生物学

Developmental Dynamics Pub Date : 2025-03-26 DOI: 10.1002/dvdy.70014

Jenna R Drummond, Raymond N Deepe, Hannah G Tarolli, Renélyn A Wolters, Inara Devji, Andrew B Harvey, Andy Wessels

{"title":"Sox9 in the second heart field and the development of the outflow tract; implications for cardiac septation and valve formation.","authors":"Jenna R Drummond, Raymond N Deepe, Hannah G Tarolli, Renélyn A Wolters, Inara Devji, Andrew B Harvey, Andy Wessels","doi":"10.1002/dvdy.70014","DOIUrl":"https://doi.org/10.1002/dvdy.70014","url":null,"abstract":"Background: Previously, we explored the role of Sox9 in the second heart field (SHF) in atrioventricular septation. For that study, we created a SHF-specific Sox9 knockout mouse. In addition to the presence of primary atrial septal defects in half of the offspring, we found that virtually all specimens also developed a ventricular septal defect. Histological analysis suggested that the ventricular septal defects resulted from developmental perturbation of the mesenchymal structures within the outflow tract. In the current study, we investigated the role of Sox9 in the SHF in the development of these tissues.Results: Sox9 is expressed in all mesenchymal cell populations in the developing outflow tract, including a cohort of endocardial-derived cells that originate from the SHF-derived endocardium. SHF-specific deletion of Sox9 inhibits the formation of this cell population and ultimately leads to truncation of the mesenchymal outlet septum. This prevents complete fusion of this outlet septum with the atrioventricular mesenchymal complex, resulting in ventricular septal defects.Conclusions: In combination with our first paper on the role of Sox9 in atrioventricular septation, data presented in this study demonstrate that Sox9 expression in the SHF is of critical importance for the proper formation of the septal structures in the developing heart.","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrating regenerative biology with developmental psychobiology to understand behavioral recovery. 结合再生生物学和发展心理生物学来理解行为康复。

IF 2 3区生物学

Developmental Dynamics Pub Date : 2025-03-25 DOI: 10.1002/dvdy.70021

Justin A Varholick

{"title":"Integrating regenerative biology with developmental psychobiology to understand behavioral recovery.","authors":"Justin A Varholick","doi":"10.1002/dvdy.70021","DOIUrl":"https://doi.org/10.1002/dvdy.70021","url":null,"abstract":"Developmental psychobiology (DPB) is a sub-discipline of developmental biology investigating the roles of physiology, biomechanics, and the environment on behavioral development. Regenerative biology is also a sub-discipline of developmental biology, studying how tissues and organs heal and regenerate after injury. One aspect of healing and regeneration is the behavioral recovery of the whole organism, involving the nervous system and coordinated movements in three-dimensional space. Behavioral recovery is often a secondary measure in many regeneration studies, primarily focusing on molecular and cellular mechanisms involved in structural recovery. Studies and frameworks in DPB, however, suggest that behaviors may have an active role in the regeneration process, and integrating regenerative biology with DPB would provide a basis for behavioral research on regenerative systems as a separate biological question to increase our understanding of behavioral recovery. Here, I introduce the probabilistic epigenesis framework from DPB and elaborate on how it reveals gaps in our knowledge concerning regeneration and behavioral recovery. I close with an initial regenerative history framework to guide regenerative biologists and bioengineers studying behavioral recovery to address these gaps and optimize behavioral recovery with regenerating tissue.","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143699978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic changes during cardiac regeneration in the axolotl. 蝾螈心脏再生过程中的代谢变化。

IF 2 3区生物学

Developmental Dynamics Pub Date : 2025-03-22 DOI: 10.1002/dvdy.70020

Anita Dittrich, Sofie Amalie Andersson, Morten Busk, Kasper Hansen, Casper Bindzus Foldager, Johan Palmfeldt, Asger Andersen, Michael Pedersen, Mikkel Vendelbo, Kirstine Lykke Nielsen, Henrik Lauridsen

{"title":"Metabolic changes during cardiac regeneration in the axolotl.","authors":"Anita Dittrich, Sofie Amalie Andersson, Morten Busk, Kasper Hansen, Casper Bindzus Foldager, Johan Palmfeldt, Asger Andersen, Michael Pedersen, Mikkel Vendelbo, Kirstine Lykke Nielsen, Henrik Lauridsen","doi":"10.1002/dvdy.70020","DOIUrl":"https://doi.org/10.1002/dvdy.70020","url":null,"abstract":"Background: The axolotl is a prominent model organism of heart regeneration due to its ability to anatomically and functionally repair the heart after an injury that mimics human myocardial infarction. In humans, such an injury leads to permanent scarring. Cardiac regeneration has been linked to metabolism and the oxygenation state, but so far, these factors remain to be detailed in the axolotl model. In this descriptive study, we have investigated metabolic changes that occurred during cardiac regeneration in the axolotl.Results: We describe systemic and local cardiac metabolic changes after injury involving an early upregulation of glucose uptake and nucleotide biosynthesis followed by a later increase in acetate uptake. We detect several promising factors and metabolites for future studies and show that, unlike other popular animal models capable of intrinsic regeneration, the axolotl maintains its cardiac regenerative ability under hyperoxic conditions.Conclusions: Axolotls undergo dynamic metabolic changes during the process of heart regeneration and display a robust reparative response to cardiac cryo-injury, which is unaffected by hyperoxia.","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143676914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LncRNA SNHG1 regulates muscle stem cells fate through Wnt/β-catenin pathway. LncRNA SNHG1通过Wnt/β-catenin通路调控肌肉干细胞命运。

IF 2 3区生物学

Developmental Dynamics Pub Date : 2025-03-21 DOI: 10.1002/dvdy.70017

Changying Wang, Wenwen Wu, Junyi Chen, Heng Wang, Pengxiang Zhao

{"title":"LncRNA SNHG1 regulates muscle stem cells fate through Wnt/β-catenin pathway.","authors":"Changying Wang, Wenwen Wu, Junyi Chen, Heng Wang, Pengxiang Zhao","doi":"10.1002/dvdy.70017","DOIUrl":"https://doi.org/10.1002/dvdy.70017","url":null,"abstract":"Background: Skeletal muscle stem cells (MuSCs) played an important role in maintaining the proper function of muscle tissues. In adults, they normally remained in a quiescent state and activated upon stimulation to undergo self-renewal or myogenic differentiation. This process was complexly regulated by cytokines, and the molecular mechanisms that promoted MuSCs activation remained largely unknown.Results: Here, we analyzed transcriptome data from MuSCs activated by different stimuli using weighted gene co-expression network analysis (WGCNA) and identified the key long non-coding RNA SNHG1 (lncSNHG1), which promotes the transition from the quiescent to the activated state of MuSCs. Overexpression of lncSNHG1 was able to promote the proliferation and differentiation of MuSCs, whereas knockdown resulted in the opposite results. Mechanistically, the disruption of the Wnt/β-catenin pathway blocked the quiescence exit induced by lncSNHG1.Conclusions: We conclude that lncSNHG1 is a key factor that promotes the transition from the quiescent to the activated state of MuSCs and promotes cell proliferation and differentiation through the Wnt/β-catenin pathway.","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A head start: The relationship of placental factors to craniofacial and brain development. 领先：胎盘因素与颅面和大脑发育的关系。

IF 2 3区生物学

Developmental Dynamics Pub Date : 2025-03-19 DOI: 10.1002/dvdy.70018

Annemarie Jenna Carver, Martine Dunnwald, Hanna Elizabeth Stevens

{"title":"A head start: The relationship of placental factors to craniofacial and brain development.","authors":"Annemarie Jenna Carver, Martine Dunnwald, Hanna Elizabeth Stevens","doi":"10.1002/dvdy.70018","DOIUrl":"https://doi.org/10.1002/dvdy.70018","url":null,"abstract":"In recent years, the importance of placental function for fetal neurodevelopment has become increasingly studied. This field, known as neuroplacentology, has greatly expanded possible etiologies of neurodevelopmental disorders by exploring the influence of placental function on brain development. It is also well-established that brain development is influenced by craniofacial morphogenesis. However, there is less focus on the impact of the placenta on craniofacial development. Recent research suggests the functional influence of placental nutrients and hormones on craniofacial skeletal growth, such as prolactin, growth hormone, insulin-like growth factor 1, vitamin D, sulfate, and calcium, impacting both craniofacial and brain development. Therefore, interactions between the placenta and both fetal neurodevelopment and craniofacial development likely influence the growth and morphology of the head as a whole. This review discusses the role of placental hormone production and nutrient delivery in the development of the fetal head-defined as craniofacial and brain tissue together-expanding on the more established focus on brain development to also include the skull (or cranium) and face.","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143656302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neural induction: New insight into the default model and an extended four-step model in vertebrate embryos. 神经诱导：对脊椎动物胚胎默认模型和扩展四步模型的新见解。

IF 2 3区生物学

Developmental Dynamics Pub Date : 2025-03-19 DOI: 10.1002/dvdy.70002

Mohsen Sagha

引用次数: 0

Interspecific comparisons of anuran embryonic epidermal landscapes and energetic trade-offs in response to changes in salinity. 无尾龙胚胎表皮景观的种间比较和对盐度变化的能量权衡。

IF 2 3区生物学

Developmental Dynamics Pub Date : 2025-03-17 DOI: 10.1002/dvdy.70016

Kourtnie Whitfield, Erica J Crespi

{"title":"Interspecific comparisons of anuran embryonic epidermal landscapes and energetic trade-offs in response to changes in salinity.","authors":"Kourtnie Whitfield, Erica J Crespi","doi":"10.1002/dvdy.70016","DOIUrl":"https://doi.org/10.1002/dvdy.70016","url":null,"abstract":"Background: Freshwater salinization is an emerging stressor in amphibian populations, and embryonic stages are most vulnerable. To better understand the variation in embryonic osmoregulation, we challenged embryos of two phylogenetically diverse anuran species, Xenopus laevis and Lithobates (Rana) sylvaticus, along a gradient of non-lethal salinities. We hypothesized embryos at higher salinities will display epidermal plasticity as a coping response and increase energy expenditure related to osmoregulation demands, thereby reducing energy for growth and development.Results: Scanning electron microscopy revealed an extra mucus-secreting cell type and higher ionocyte proportions in the X. laevis epidermis, suggesting more osmoregulatory machinery than L. sylvaticus. Under elevated salinity, X. laevis displayed greater increases in goblet cell proportions, mucus secretion, and reductions in ionocyte apical area compared with L. sylvaticus. Although both species increased oxygen consumption rates and reduced body length with elevated salinity, these effects were proportionally greater in L. sylvaticus at the highest salinity, and only this species slowed developmental rates.Conclusion: These findings support the hypothesis that frog embryos respond to salinity by altering the cellular landscape of their epidermis. We show that epidermal cell types, as well as the magnitude of epidermal plasticity and energetic trade-offs in response to salinity, vary among amphibian species.","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143647400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of the PTHrP/Ihh feedback loop in the unusual growth plate location in mammalian metatarsals and pisiforms. PTHrP/Ihh反馈回路在哺乳动物跖骨和肾状骨异常生长板位置中的作用

IF 2 3区生物学

Developmental Dynamics Pub Date : 2025-03-15 DOI: 10.1002/dvdy.70013

Philip L Reno, Sherrie Wallace, Sarah N Doelp, Maria Biancaniello, Kelsey M Kjosness

{"title":"The role of the PTHrP/Ihh feedback loop in the unusual growth plate location in mammalian metatarsals and pisiforms.","authors":"Philip L Reno, Sherrie Wallace, Sarah N Doelp, Maria Biancaniello, Kelsey M Kjosness","doi":"10.1002/dvdy.70013","DOIUrl":"https://doi.org/10.1002/dvdy.70013","url":null,"abstract":"Background: Longitudinal skeletal growth takes place in the cartilaginous growth plates. While growth plates are found at either end of conventional long bones, they occur at a variety of locations in the mammalian skeleton. For example, the metacarpals and metatarsals (MT) in the hands and feet form only a single growth plate at one end, and the pisiform in the wrist is the only carpal bone to contain a growth plate. We take advantage of this natural anatomical variation to test which components of the PTHrP/Ihh feedback loop, a fundamental regulator of chondrocyte differentiation, are specific to growth plate function.Results: Parathyroid hormone-like hormone (Pthlh), the gene that transcribes parathyroid hormone-related peptide (PTHrP), is expressed in the reserve zone of the growth plate-forming end of the MT. At the opposite end, the absence of a PTHrP+ reserve zone results in premature chondrocyte differentiation and Indian hedgehog (Ihh) expression. Pthlh is expressed in the reserve zone of the developing pisiform, confirming the existence of a true growth plate.Conclusion: A pool of PTHrP+ reserve zone chondrocytes is a defining characteristic of growth plates, and its patterning may be key to evolved differences in growth plate location in the mammalian skeleton.","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Squamate ventricular cardiomyocytes: Ploidy, proliferation, and heart muscle cell size in the leopard gecko (Eublepharis macularius). 鳞状心室心肌细胞：豹纹壁虎（Eublepharis macularius）的倍性、增殖和心肌细胞大小。

IF 2 3区生物学

Developmental Dynamics Pub Date : 2025-03-15 DOI: 10.1002/dvdy.70015

Kathy Jacyniak, Karemna Barrera Jaimes, Minh Hanh Doan, Jordyn M Chartrand, Matthew K Vickaryous

{"title":"Squamate ventricular cardiomyocytes: Ploidy, proliferation, and heart muscle cell size in the leopard gecko (Eublepharis macularius).","authors":"Kathy Jacyniak, Karemna Barrera Jaimes, Minh Hanh Doan, Jordyn M Chartrand, Matthew K Vickaryous","doi":"10.1002/dvdy.70015","DOIUrl":"https://doi.org/10.1002/dvdy.70015","url":null,"abstract":"Background: While heart function is broadly conserved across vertebrates, the cellular phenotype of muscle cells (cardiomyocytes) varies across taxa and throughout ontogeny. Emerging evidence suggests that some attributes may correlate with the capacity for spontaneous cardiomyocyte replacement following injury. For example, among non-regenerating taxa like adult mammals and birds, cardiomyocytes are polyploid, rarely proliferate, and are large in size. In contrast, in regeneration-competent zebrafish and amphibians, cardiomyocytes are diploid, spontaneously proliferate, and are comparatively small. For other species, less is known.Results: Here, we investigate these attributes in the squamate Eublepharis macularius, the leopard gecko. Using the nuclear counterstain DAPI to measure fluorescence intensity as a proxy for DNA content, we found that >90% of adult cardiomyocytes are diploid. Using serial histology and immunostaining for markers of DNA synthesis and mitosis, we determined that adult gecko cardiomyocytes spontaneously proliferate, albeit at significantly lower levels than previously reported in subadults. Furthermore, using wheat germ agglutinin, we found that the cross-sectional area is maintained across ontogeny and that gecko cardiomyocytes are 10× smaller than those of mice.Conclusions: Taken together, our data show that gecko cardiomyocytes share several key cellular attributes with regeneration-competent species and that postnatal ventricular growth occurs via cardiomyocyte hyperplasia.","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamics of primary cilia in endothelial and mesenchymal cells throughout mouse lung development. 内皮细胞和间充质细胞中原代纤毛在小鼠肺发育过程中的动态。

IF 2 3区生物学

Developmental Dynamics Pub Date : 2025-03-08 DOI: 10.1002/dvdy.70008

Stephen Spurgin, Ange Michelle Nguimtsop, Fatima N Chaudhry, Sylvia N Michki, Jocelynda Salvador, M Luisa Iruela-Arispe, Jarod A Zepp, Saikat Mukhopadhyay, Ondine Cleaver

{"title":"Dynamics of primary cilia in endothelial and mesenchymal cells throughout mouse lung development.","authors":"Stephen Spurgin, Ange Michelle Nguimtsop, Fatima N Chaudhry, Sylvia N Michki, Jocelynda Salvador, M Luisa Iruela-Arispe, Jarod A Zepp, Saikat Mukhopadhyay, Ondine Cleaver","doi":"10.1002/dvdy.70008","DOIUrl":"10.1002/dvdy.70008","url":null,"abstract":"Cilia are specialized structures found on a variety of mammalian cells, with variable roles in the transduction of mechanical and biological signals (by primary cilia, PC), as well as in the generation of fluid flow (by motile cilia). Their critical role in the establishment of a left-right axis in early development is well described, as well as in the defense immune function of multiciliated upper airway epithelium. By contrast, detailed analysis of the ciliary status of specific cell types during organogenesis and postnatal development has received less attention. In this study, we investigate the progression of ciliary status within the endothelium and mesenchyme of the lung. Remarkably, we find that pulmonary endothelial cells (ECs) lack PC at all stages of development, except in low numbers in the proximal portions of older pulmonary arteries. Mesenchymal cells, by contrast, widely exhibit PC in early development, and a large subset of PDGFRα+ fibroblasts maintain PC into adulthood. The dynamic and differential ciliation of multiple cellular populations in the developing lung both challenges prior assertions that PC are found on all cells and highlights a need to understand their spatiotemporal functions.","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143583722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0