Developmental Dynamics最新文献

{"title":"Morphologically defined substages of tail morphogenesis in C. elegans males","authors":"Karin Kiontke,&nbsp;Porfirio Fernandez,&nbsp;Alyssa Woronik,&nbsp;David H. A. Fitch","doi":"10.1002/dvdy.721","DOIUrl":"10.1002/dvdy.721","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Sex-specific morphogenesis occurs in <i>Caenorhabditis elegans</i> in the vulva of the hermaphrodite and in the male tail during the last larval stage. Temporal progression of vulva morphogenesis has been described in fine detail. However, a similar precise description of male tail morphogenesis was lacking.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>We here describe morphogenesis of the male tail at time points matching vulva development with special focus on morphogenesis of the tail tip. Using fluorescent reporters, we follow changes in cell shapes, cell fusions, nuclear migration, modifications in the basement membrane, and formation of a new apical extracellular matrix at the end of the tail.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Our analysis answers two open questions about tail tip morphogenesis (TTM) by showing that one of the four tail tip cells, hyp11, remains largely separate, while the other cells fully fuse with each other and with two additional tail cells to form a ventral tail syncytium. This merger of cells begins at the apical surface early during TTM but is only completed toward the end of the process. This work provides a framework for future investigations of cell biological factors that drive male tail morphogenesis.</p>\u0000 </section>\u0000 </div>","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":"253 12","pages":"1147-1164"},"PeriodicalIF":2.0,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/dvdy.721","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141455860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The dishevelled associated activator of morphogenesis protein 2 (Daam2) regulates neural tube closure","authors":"Kaushik Nama,&nbsp;Baihao Su,&nbsp;Jonathan Marquez,&nbsp;Mustafa K. Khokha,&nbsp;Raymond Habas","doi":"10.1002/dvdy.720","DOIUrl":"10.1002/dvdy.720","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>The Wnt signaling pathway is highly conserved in metazoans and regulates a large array of cellular processes including motility, polarity and fate determination, and stem cell homeostasis. Modulation of the actin cytoskeleton via the non-canonical Wnt pathway regulate cell polarity and cell migration that are required for proper vertebrate gastrulation and subsequent neurulation. However, the mechanism(s) of how the non-canonical pathway mediates actin cytoskeleton modulation is not fully understood.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Herein, we characterize the role of the Formin-homology protein; dishevelled associated activator of morphogenesis 2 (Daam2) protein in the Wnt signaling pathway. Co-immunoprecipitation assays confirm the binding of Daam2 to dishevelled2 (Dvl2) as well as the domains within these proteins required for interaction; additionally, the interaction between Daam2 and Dvl2 was Wnt-regulated. Sub-cellular localization studies reveal Daam2 is cytoplasmic and regulates the cellular actin cytoskeleton by modulating actin filament formation. During <i>Xenopus</i> development, a knockdown or loss of Daam2 specifically produces neural tube closure defects indicative of a role in non-canonical signaling. Additionally, our studies did not identify any role for Daam2 in canonical Wnt signaling in mammalian culture cells or the <i>Xenopus</i> embryo.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Our studies together identify Daam2 as a component of the non-canonical Wnt pathway and Daam2 is a regulator of neural tube morphogenesis during vertebrate development.</p>\u0000 </section>\u0000 </div>","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":"253 12","pages":"1130-1146"},"PeriodicalIF":2.0,"publicationDate":"2024-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141327382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptional regulators with broad expression in the zebrafish spinal cord","authors":"Samantha J. England,&nbsp;Paul C. Campbell,&nbsp;Santanu Banerjee,&nbsp;Richard L. Bates,&nbsp;Ginny Grieb,&nbsp;William F. Fancher,&nbsp;Katharine E. Lewis","doi":"10.1002/dvdy.717","DOIUrl":"10.1002/dvdy.717","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>The spinal cord is a crucial part of the vertebrate CNS, controlling movements and receiving and processing sensory information from the trunk and limbs. However, there is much we do not know about how this essential organ develops. Here, we describe expression of 21 transcription factors and one transcriptional regulator in zebrafish spinal cord.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>We analyzed the expression of <i>aurkb</i>, <i>foxb1a</i>, <i>foxb1b</i>, <i>her8a</i>, <i>homeza</i>, <i>ivns1abpb</i>, <i>mybl2b</i>, <i>myt1a</i>, <i>nr2f1b</i>, <i>onecut1</i>, <i>sall1a</i>, <i>sall3a</i>, <i>sall3b</i>, <i>sall4</i>, <i>sox2</i>, <i>sox19b</i>, <i>sp8b</i>, <i>tsc22d1</i>, <i>wdhd1</i>, <i>zfhx3b</i>, <i>znf804a</i>, and <i>znf1032</i> in wild-type and <i>MIB E3 ubiquitin protein ligase 1</i> zebrafish embryos. While all of these genes are broadly expressed in spinal cord, they have distinct expression patterns from one another. Some are predominantly expressed in progenitor domains, and others in subsets of post-mitotic cells. Given the conservation of spinal cord development, and the transcription factors and transcriptional regulators that orchestrate it, we expect that these genes will have similar spinal cord expression patterns in other vertebrates, including mammals and humans.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Our data identify 22 different transcriptional regulators that are strong candidates for playing different roles in spinal cord development. For several of these genes, this is the first published description of their spinal cord expression.</p>\u0000 </section>\u0000 </div>","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":"253 11","pages":"1036-1055"},"PeriodicalIF":2.0,"publicationDate":"2024-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/dvdy.717","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141293280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial highlights","authors":"Paul A. Trainor","doi":"10.1002/dvdy.718","DOIUrl":"10.1002/dvdy.718","url":null,"abstract":"&lt;p&gt;Every organism is a model organism for understanding development, evolution, disease, and regeneration, and we have only begun to scratch the surface of the interdisciplinary genetic, molecular, cellular, and developmental mechanisms that regulate these biological processes. These “Highlights” denote exciting advances recently reported in &lt;i&gt;Developmental Dynamics&lt;/i&gt; that illustrate the complex dynamics of developmental biology.&lt;/p&gt;&lt;p&gt;&lt;b&gt;Mechanical Forces in Development&lt;/b&gt; “Exploring the Role of Mechanical Forces on Tendon Development Using in vivo Model: A Scoping Review,” by Yuna Usami, Hirotaka Iijima, Takanori Kokubun; &lt;i&gt;DevDyn&lt;/i&gt; 253:6, pp. 550-565. https://doi.org/10.1002/dvdy.673. Tendons comprise connective tissue that transmits muscle contraction forces to bones and drives joint movement throughout life. Interestingly, however, mechanical forces also regulate and control key cellular and molecular responses during musculoskeletal tissue development, differentiation, and growth. Scleraxis (Scx), a basic helix–loop–helix (bHLH) transcription factor, is the most representative marker of tendon development and most studies have focused on the loss of muscle, muscle dysfunction, and weight-bearing regulation, but few investigated the effect of increased mechanical force. This review summarizes our current knowledge about animal models and approaches for modulating mechanical forces on tendon development; defines the role of mechanical force through the activity of Scx and other tendon development-associated factors; and raises important questions and directions for the field to address in the future.&lt;/p&gt;&lt;p&gt;&lt;b&gt;Zebrafish Fin Development&lt;/b&gt; “wnt10a is required for zebrafish median fin fold maintenance and adult unpaired fin metamorphosis” by Erica Benard, Ismail Küçükaylak, Julia Hatzold, Kilian Berendes, Thomas J. Carney, Filippo Beleggia, and Matthias Hammerschmidt; &lt;i&gt;DevDyn&lt;/i&gt; 253:6, pp. 566-592. https://doi.org/10.1002/dvdy.672.&lt;/p&gt;&lt;p&gt;Wnt signaling regulates critical cell-to-cell interactions in multiple developmental processes during embryogenesis and in the homeostasis of adult tissues. Mutations in human &lt;i&gt;WNT10A&lt;/i&gt; are associated with odonto-ectodermal dysplasia syndromes, which are primarily characterized by severe oligodontia of permanent teeth, and skin anomalies. In this study, the authors generate &lt;i&gt;wnt10a&lt;/i&gt; mutant zebrafish embryos, which display impaired tooth development and a collapsing median fin fold (MFF), making them a good model for odonto-onycho-dermal dysplasia. Focusing on the MFF, &lt;i&gt;dlx2a&lt;/i&gt; activity was found to be decreased in the distal-most cells, together with perturbed expression of &lt;i&gt;col1a1a&lt;/i&gt; and other extracellular matrix proteins encoding genes. Consequently, positioning of actinotrichia within the cleft of distal MFF cells become compromised, coinciding with actinotrichia shrinkage and MFF collapse. Rescue experiments demonstrate that wnt10a is essential for MFF maintenance, both during em","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":"253 6","pages":"548-549"},"PeriodicalIF":2.5,"publicationDate":"2024-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/dvdy.718","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141199453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteomic profiling of TBC1 domain family member 21-null sperms reveals the critical roles of TEKT 1 in their tail defects","authors":"Pei-Yi Pan,&nbsp;Chih-Chun Ke,&nbsp;Ya-Yun Wang,&nbsp;Yu-Hua Lin,&nbsp;Wei-Chi Ku,&nbsp;Chin-Fong Au,&nbsp;Chying-Chyuan Chan,&nbsp;Chia-Yen Huang,&nbsp;Ying-Hung Lin","doi":"10.1002/dvdy.716","DOIUrl":"10.1002/dvdy.716","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Approximately 7% of the males exhibit reduced fertility; however, the regulatory genes and pathways involved remain largely unknown. TBC1 domain family member 21 (TBC1D21) contains a conserved RabGAP catalytic domain that induces GDP/GTP exchange to inactivate Rabs by interacting with microtubules. We previously reported that <i>Tbc1d21-null</i> mice exhibit severe sperm tail defects with a disrupted axoneme, and that TBC1D21 interacts with RAB10. However, the pathological mechanisms underlying the <i>Tbc1d21</i> loss<i>-</i>induced sperm tail defects remain unknown.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Murine sperm from wild-type and <i>Tbc1d21</i>-null mice were comparatively analyzed using proteomic assays. Over 1600 proteins were identified, of which 15 were significantly up-regulated in <i>Tbc1d21</i>-null sperm. Notably, several tektin (TEKT) family proteins, belonging to a type of intermediate filament critical for stabilizing the microtubular structure of cilia and flagella, were significantly up-regulated in <i>Tbc1d21</i>^{<i>−/−</i>} sperm. We also found that TBC1D21 interacts with TEKT1. In addition, TEKT1 co-localized with RAB10 during sperm tail formation. Finally, we found <i>Tbc1d21</i>-null sperm exhibited abnormal accumulation of TEKT1 in the midpiece region, accompanied by disrupted axonemal structures.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>These results reveal that TBC1D21 modulates TEKTs protein localization in the axonemal transport system during sperm tail formation.</p>\u0000 </section>\u0000 </div>","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":"253 11","pages":"1024-1035"},"PeriodicalIF":2.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141186478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of node architecture and emergence of molecular organizer characteristics in the pig embryo.","authors":"Braah Harmoush, Christoph Viebahn, Nikoloz Tsikolia","doi":"10.1002/dvdy.715","DOIUrl":"https://doi.org/10.1002/dvdy.715","url":null,"abstract":"<p><strong>Background: </strong>The avian node is the equivalent of the amphibian Spemann's organizer, as indicated by its ability to induce a secondary axis, cellular contribution, and gene expression, whereas the node of the mouse, which displays limited inductive capacities, was suggested to be a part of spatially distributed signaling. Furthermore, the structural identity of the mouse node is subject of controversy, while little is known about equivalent structures in other mammals.</p><p><strong>Results: </strong>We analyzed the node and emerging organizer in the pig using morphology and the expression of selected organizer genes prior to and during gastrulation. The node was defined according to the \"four-quarter model\" based on comparative consideration. The node of the pig displays a multilayered, dense structure that includes columnar epithelium, bottle-like cells in the dorsal part, and mesenchymal cells ventrally. Expression of goosecoid (gsc), chordin, and brachyury, together with morphology, reveal the consecutive emergence of three distinct domains: the gastrulation precursor domain, the presumptive node, and the mature node. Additionally, gsc displays a ventral expression domain prior to epiblast epithelialization.</p><p><strong>Conclusion: </strong>Our study defines the morphological and molecular context of the emerging organizer equivalent in the pig and suggests a sequential development of its function.</p>","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140908490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stress granule related-genes during blastogenetic cycle of two colonial ascidians: Botryllus schlosseri compared to Botryllus primigenus.","authors":"Laura Drago, Takumi Sasada, Takeshi Sunanaga, Loriano Ballarin","doi":"10.1002/dvdy.714","DOIUrl":"https://doi.org/10.1002/dvdy.714","url":null,"abstract":"","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140897699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Embryology of the fat-tailed dunnart (Sminthopsis crassicaudata): A marsupial model for comparative mammalian developmental and evolutionary biology.","authors":"Axel H Newton, Jennifer C Hutchison, Ella R Farley, Emily L Scicluna, Neil A Youngson, Jun Liu, Brandon R Menzies, Thomas B Hildebrandt, Ben M Lawrence, Angus H W Sutherland, David L Potter, Gerard A Tarulli, Lynne Selwood, Stephen Frankenberg, Sara Ord, Andrew J Pask","doi":"10.1002/dvdy.711","DOIUrl":"https://doi.org/10.1002/dvdy.711","url":null,"abstract":"<p><strong>Background: </strong>Marsupials are a diverse and unique group of mammals, but remain underutilized in developmental biology studies, hindering our understanding of mammalian diversity. This study focuses on establishing the fat-tailed dunnart (Sminthopsis crassicaudata) as an emerging laboratory model, providing reproductive monitoring methods and a detailed atlas of its embryonic development.</p><p><strong>Results: </strong>We monitored the reproductive cycles of female dunnarts and established methods to confirm pregnancy and generate timed embryos. With this, we characterized dunnart embryo development from cleavage to birth, and provided detailed descriptions of its organogenesis and heterochronic growth patterns. Drawing stage-matched comparisons with other species, we highlight the dunnarts accelerated craniofacial and limb development, characteristic of marsupials.</p><p><strong>Conclusions: </strong>The fat-tailed dunnart is an exceptional marsupial model for developmental studies, where our detailed practices for reproductive monitoring and embryo collection enhance its accessibility in other laboratories. The accelerated developmental patterns observed in the Dunnart provide a valuable system for investigating molecular mechanisms underlying heterochrony. This study not only contributes to our understanding of marsupial development but also equips the scientific community with new resources for addressing biodiversity challenges and developing effective conservation strategies in marsupials.</p>","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140890605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial highlights","authors":"","doi":"10.1002/dvdy.713","DOIUrl":"https://doi.org/10.1002/dvdy.713","url":null,"abstract":"&lt;p&gt;Every organism is a model organism for understanding development, evolution, disease, and regeneration, and we have only begun to scratch the surface of the interdisciplinary genetic, molecular, cellular, and developmental mechanisms that regulate these biological processes. These “Highlights” denote exciting advances recently reported in &lt;i&gt;Developmental Dynamics&lt;/i&gt; that illustrate the complex dynamics of developmental biology.&lt;/p&gt;&lt;p&gt;&lt;b&gt;Preimplantation Mammalian Development.&lt;/b&gt; “Maternal exposure to hyperbaric oxygen at the preimplantation stages increases apoptosis and ectopic &lt;i&gt;Cdx2&lt;/i&gt; expression and decreases &lt;i&gt;Oct4&lt;/i&gt; expression in mouse blastocysts via Nrf2-Notch1 upregulation and Nf2 downregulation” by Yu-Ming Li, Yu Lang Chung, Yung-Fu Wu, Chien-Kuo Wang, Chieh-Min Chen, and Yi-Hui Chen; &lt;i&gt;DevDyn&lt;/i&gt; 253:5, pp. 467–489. https://doi.org/10.1002/dvdy.671. Environmental oxygen tension during preimplantation development in vivo and in vitro is a critical regulator of blastomere cleavage, blastocyst implantation, and pregnancy. Therefore, the use of hyperbaric oxygen in pregnant women is limited due to potential adverse risks. In this study, maternal hyperbaric oxygen exposure during preimplantation embryo development, resulted in increased DNA damage and apoptosis in the inner cell mass, abnormal lineage specification, and impaired lineage segregation between the inner cell mass and trophectoderm. The domain and levels of both &lt;i&gt;Nf2&lt;/i&gt; and &lt;i&gt;Yap&lt;/i&gt; gene expression, are pivotal regulators of early lineage segregations in both mouse and human preimplantation embryos and here the authors show that Nf2-Yap and Nrf2-Notch1 signaling are two critical regulatory pathways that mediate hyperbaric oxygen-induced aberrant lineage specification. Thus, hyperbaric oxygen-induced oxidative stress is associated with aberrant first lineage segregation in the preimplantation embryo development.&lt;/p&gt;&lt;p&gt;&lt;b&gt;Retina Development.&lt;/b&gt; “&lt;i&gt;Kdm7a&lt;/i&gt; expression is spatiotemporally regulated in developing &lt;i&gt;Xenopus laevis&lt;/i&gt; embryos, and its overexpression influences late retinal development” by Davide Martini, Matteo Digregorio, Ilaria Anna Pia Voto, Giuseppe Morabito, Andrea Degl'Innocenti, Guido Giudetti, Martina Giannaccini and Massimiliano Andreazzoli; &lt;i&gt;DevDyn&lt;/i&gt; 253:5, pp. 508–518. https://doi.org/10.1002/dvdy.670. Epigenetics is the study of complex and dynamically reversible chemical modification of DNA and histone proteins that remodel heterochromatin and euchromatin, and their effects on gene transcription. Posttranslational histone modifications are among the most common epigenetic modifications that regulate gene activity during embryonic development and in the pathogenesis of disease. KDM7A is a histone lysine demethylase that catalyzes the demethylation of H3K9me1/2 and H3K27me1/2. This study shows that &lt;i&gt;kdm7a&lt;/i&gt; is dynamically expressed during embryonic development, and that overexpression of &lt;i&gt;kdm7a&lt;/i&gt; alters the late stages of ret","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":"253 5","pages":"452"},"PeriodicalIF":2.5,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/dvdy.713","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140820497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Muscular remodeling and anteroposterior patterning during tapeworm segmentation","authors":"Francesca Jarero,&nbsp;Andrew Baillie,&nbsp;Nick Riddiford,&nbsp;Jimena Montagne,&nbsp;Uriel Koziol,&nbsp;Peter D. Olson","doi":"10.1002/dvdy.712","DOIUrl":"10.1002/dvdy.712","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Tapeworms are parasitic flatworms that independently evolved a segmented body plan, historically confounding comparisons with other animals. Anteroposterior (AP) patterning in free-living flatworms and in tapeworm larvae is associated with canonical Wnt signaling and positional control genes (PCGs) are expressed by their musculature in regionalized domains along the AP axis. Here, we extend investigations of PCG expression to the adult of the mouse bile-duct tapeworm <i>Hymenolepis microstoma</i>, focusing on the growth zone of the neck region and the initial establishment of segmental patterning.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>We show that the adult musculature includes new, segmental elements that first appear in the neck and that the spatial patterns of Wnt factors are consistent with expression by muscle cells. Wnt factor expression is highly regionalized and becomes AP-polarized in segments, marking them with axes in agreement with the polarity of the main body axis, while the transition between the neck and strobila is specifically demarcated by the expression domain of a Wnt11 paralog.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>We suggest that segmentation could originate in the muscular system and participate in patterning the AP axis through regional and polarized expression of PCGs, akin to the gene regulatory networks employed by free-living flatworms and other animals.</p>\u0000 </section>\u0000 </div>","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":"253 11","pages":"998-1023"},"PeriodicalIF":2.0,"publicationDate":"2024-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/dvdy.712","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140836419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}