Comprehensive observation of histone lysine lactylation during gametogenesis of Drosophila melanogaster.

IF 2 3区生物学 Q2 ANATOMY & MORPHOLOGY

Developmental Dynamics Pub Date : 2025-03-28 DOI:10.1002/dvdy.70010

Yoshiki Hayashi, Ban Sato, Rio Kageyama, Kenji Miyado, Daisuke Saito, Satoru Kobayashi, Natsuko Kawano

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引用次数: 0

Abstract

Background: Histone post-translational modification (PTM) is an important epigenomic regulation content and an essential process regulating gene expression. Histone lysine lactylation is the newly identified histone PTM that utilizes the lactyl moiety for its modification. Although histone lysine lactylation is considered an essential outcome of the Wardburg effects and the interconnection between cellular metabolism and gene regulation, the developmental contexts involving this PTM are largely unknown. In this study, we comprehensively observed histone lysine lactylation during Drosophila oogenesis, one of the developmental contexts in which chromatin regulation plays crucial roles.

Results: Our study revealed that lactylation on the specific histone lysine mainly occurs in the oocyte karyosome and condensed meiotic chromosome, suggesting histone lysine lactylation has a vital role in female meiosis. Interestingly, one of the histone lysine lactylations, lactylation of lysine 14 of histone H3, is intensively observed in the meiotic germline in the mouse ovary, suggesting that lactylation has an evolutionarily conserved role.

Conclusions: Our results revealed that histone lysine lactylation is predominantly present in transcriptionally repressive meiotic chromatin, which contradicts the previously reported function of histone lactylation in transcriptional activation. This study, therefore, provides the first fundamental information to understand the role of histone lysine lactylation in the germline and repressive chromatin.

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黑腹果蝇配子体发生过程中组蛋白赖氨酸乳酸化的综合观察。

背景：组蛋白翻译后修饰（PTM）是一种重要的表观基因组调控内容，是调控基因表达的重要过程。组蛋白赖氨酸乳酸化是新发现的利用乙酰基片段进行修饰的组蛋白PTM。尽管组蛋白赖氨酸乳酸化被认为是Wardburg效应和细胞代谢与基因调控之间相互联系的重要结果，但涉及这种PTM的发育背景在很大程度上是未知的。在这项研究中，我们全面观察了果蝇卵发生过程中的组蛋白赖氨酸乳酸化，这是染色质调控起关键作用的发育背景之一。结果：我们的研究表明，特定组蛋白赖氨酸的乳酸化主要发生在卵母细胞核体和浓缩的减数分裂染色体上，提示组蛋白赖氨酸的乳酸化在雌性减数分裂中起着重要作用。有趣的是，组蛋白赖氨酸的一种乳酸化作用，即组蛋白H3的赖氨酸14的乳酸化作用，在小鼠卵巢减数分裂生殖系中被大量观察到，这表明乳酸化作用具有进化上的保守作用。结论：我们的研究结果表明，组蛋白赖氨酸乳酸化主要存在于转录抑制的减数分裂染色质中，这与先前报道的组蛋白乳酸化在转录激活中的功能相矛盾。因此，这项研究为了解组蛋白赖氨酸乳酸化在种系和抑制染色质中的作用提供了第一个基本信息。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Developmental Dynamics 生物-发育生物学

CiteScore

5.10

自引率

8.00%

发文量

116

审稿时长

3-8 weeks

期刊介绍： Developmental Dynamics, is an official publication of the American Association for Anatomy. This peer reviewed journal provides an international forum for publishing novel discoveries, using any model system, that advances our understanding of development, morphology, form and function, evolution, disease, stem cells, repair and regeneration.