Developmental and comparative immunology最新文献_第3页

miR-181c Regulates the process of the Infection of Singapore Grouper Iridovirus via targeting PDCD4 in Epinephelus coioides miR-181c通过靶向石斑鱼体内PDCD4细胞调控新加坡石斑鱼虹膜病毒的感染过程。

IF 2.4 3区农林科学

Developmental and comparative immunology Pub Date : 2026-02-01 Epub Date: 2026-01-06 DOI: 10.1016/j.dci.2026.105548

Hong Qi , Jia-Yang He , Jia-Ru Zhou , Yun-Xiang Lin , Yu-Rong Lei, Hai Zhu He, Wei-Yang Huang, Qi-Wei Qin, Hong-Yan Sun

{"title":"miR-181c Regulates the process of the Infection of Singapore Grouper Iridovirus via targeting PDCD4 in Epinephelus coioides","authors":"Hong Qi , Jia-Yang He , Jia-Ru Zhou , Yun-Xiang Lin , Yu-Rong Lei, Hai Zhu He, Wei-Yang Huang, Qi-Wei Qin, Hong-Yan Sun","doi":"10.1016/j.dci.2026.105548","DOIUrl":"10.1016/j.dci.2026.105548","url":null,"abstract":"<div><div>Recent studies found that non-coding RNA could be involved in the development of pathogen infection. In this study, the role of non-coding RNA miRNA-181c (miR-181c) response to Iridovirus SGIV (an important viral pathogen and can cause huge economic losses in marine fish industry) infection was explored in <em>Epinephelus coioides</em>, an important economic fish in South China. The results showed that SGIV infection inhibited the expression of <em>E</em>. <em>coioides</em> miR-181c. Upregulated miR-181c significantly inhibited the invasion of SGIV, the expressions of key SGIV genes (<em>MCP</em>, <em>ICP18</em>, LITAF and VP19), SGIV-induced CPE, and the titers of SGIV. Programmed cell death 4 (PDCD4) of <em>E</em>. <em>coioides</em> was a direct target of miR-181c. miR-181c could regulate the expressions of the immune- and apoptosis-related factors, and SGIV-induced apoptosis via targeting PDCD4. Downregulated miR-181c could produce the opposite results. These findings would be useful for exploring miRNAs for potentially controlling viral infection.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"175 ","pages":"Article 105548"},"PeriodicalIF":2.4,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145917372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of viral ribonucleoprotein complex genetic stability on pathogenicity in H9N2 and H6N2 avian influenza viruses 病毒核糖核蛋白复合体遗传稳定性对H9N2和H6N2禽流感病毒致病性的影响

IF 2.4 3区农林科学

Developmental and comparative immunology Pub Date : 2026-02-01 Epub Date: 2025-12-27 DOI: 10.1016/j.dci.2025.105544

Yuwen Luo , Mengyi Dong , Yuxi Shen , Xuelian Xiang , Jiadai Lv , Yi Sun , Yongxin Li , Yamei Huang , Min Cui , Xinfeng Han , Jing Xia , Yong Huang

{"title":"Impact of viral ribonucleoprotein complex genetic stability on pathogenicity in H9N2 and H6N2 avian influenza viruses","authors":"Yuwen Luo , Mengyi Dong , Yuxi Shen , Xuelian Xiang , Jiadai Lv , Yi Sun , Yongxin Li , Yamei Huang , Min Cui , Xinfeng Han , Jing Xia , Yong Huang","doi":"10.1016/j.dci.2025.105544","DOIUrl":"10.1016/j.dci.2025.105544","url":null,"abstract":"<div><div>China has a great demand for poultry, while avian influenza (AI) remains widespread and exhibits high instability, posing a persistent challenge to the poultry industry. Elucidating the genetic stability of avian influenza variants and their implications for pathogenicity is key for pandemic risk mitigation. H9 and H6 AIVs have co-circulated in China due to a specific evolutionary dynamic: H9 AIVs serve as stable genetic reservoirs, whereas H6 AIVs act as platforms for multi-subtype reassortment. <strong>This reassortment process continually generates novel variants with unpredictable virulence, posing a persistent biosecurity threat to poultry production.</strong> Genetic stability analysis of H9/H6 AI viral Ribonucleoprotein (vRNP) complexes (<em>PB2, PB1, PA, NP</em>) revealed clear subtype-specific divergence. H9 AIVs retained highly conserved gene constellations, whereas H6 AIVs exhibited pronounced plasticity, acquiring 42.5 % of <em>PB2</em>, 20.1 % of <em>PA</em>, and 23.7 % of <em>NP</em> genes from other AIV subtypes. The H9vRNP-H6 reassortant (H6N2 backbone carrying H9N2 vRNP) showed enhanced replicability in both MDCK cells (4.7 → 5.7 log<sub>10</sub>TCID<sub>50</sub>/mL) and embryonated eggs (6.7 → 7.7 log<sub>10</sub>EID<sub>50</sub>/mL), with significantly promoted pathogenicity in chicks. These findings <strong>highlight the urgent need to integrate genetic surveillance of H9 and H6 AIVs into targeted prevention frameworks</strong>, thereby forestalling the emergence of pandemic-prone reassortants and mitigating potential losses to the poultry industry and public health. Hence, such research would provide a theoretical basis for the development of novel vaccines and enhance strategies for the control and prevention of avian influenza.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"175 ","pages":"Article 105544"},"PeriodicalIF":2.4,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145855021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional characterization of short-isoform of PARP12 in the immune response to SVCV infection in common carp (Cyprinus carpio) 鲤对SVCV感染免疫应答中PARP12短亚型的功能研究

IF 2.4 3区农林科学

Developmental and comparative immunology Pub Date : 2026-02-01 Epub Date: 2026-01-13 DOI: 10.1016/j.dci.2026.105552

Ting Li, Yingying Zhang, Cuixia Wang, Ruikuan Yu, Dongchun Yan, Lingjun Si, Linrui Chang

{"title":"Functional characterization of short-isoform of PARP12 in the immune response to SVCV infection in common carp (Cyprinus carpio)","authors":"Ting Li, Yingying Zhang, Cuixia Wang, Ruikuan Yu, Dongchun Yan, Lingjun Si, Linrui Chang","doi":"10.1016/j.dci.2026.105552","DOIUrl":"10.1016/j.dci.2026.105552","url":null,"abstract":"<div><div>Poly(ADP-ribose) polymerase 12 (PARP12), which has been identified as an interferon-stimulated gene (ISG), is able to restrict virus replication via PARP-dependent ADP-ribosylation of target molecules. Presently, the antiviral abilities of PARP12 have been reported in mammalian organisms, whereas no data is available on the immune function of PARP12 in fish species. In the present study, a PARP12 gene (CcPARP12) was identified from common carp (<em>Cyprinus carpio</em>), and its role in antiviral immune response was investigated. Structurally, CcPARP12 was shown to consist of ZnF-C3H1 motifs and WWE domain, which was in line with PARP12-short (S) isoform in domain architecture. <em>In vivo</em> gene expression analysis showed that CcPARP12 mRNA expression levels were varied in various tissues of healthy carps with the highest level in head kidney, and spring viraemia of carp virus (SVCV) induced CcPARP12 expression in multiple immune-related tissues. Analysis of subcellular localization showed that CcPARP12 was located in the nucleus region of EPC cells. Additionally, <em>in vitro</em>, it was shown that CcPARP12 could affect SVCV replication in EPC cells, with reduced virus load and relieved cytopathic effects (CPEs), and moreover positively regulated the expressions of antiviral molecules ISG15, IFN and Viperin in response to SVCV infection. Overall, these results indicated that CcPARP12 could play the defensive role in the host immune response to SVCV infection. This study has broadened the understanding of antiviral properties of PARP12 in fish species and even lower vertebrates, providing potential new therapeutic targets against SVCV infection.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"175 ","pages":"Article 105552"},"PeriodicalIF":2.4,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145974723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and functional characterization of a circular RNA circeIF4G3: a negative regulator of innate immunity in miiuy croaker (Miichthys miiuy) 环状RNA circeIF4G3的鉴定和功能表征：小斑马鱼先天免疫负调节因子。

IF 2.4 3区农林科学

Developmental and comparative immunology Pub Date : 2026-01-01 Epub Date: 2025-12-03 DOI: 10.1016/j.dci.2025.105537

Yanqiu Cui , Yaqi Tao , Tianjun Xu , Yuena Sun

{"title":"Identification and functional characterization of a circular RNA circeIF4G3: a negative regulator of innate immunity in miiuy croaker (Miichthys miiuy)","authors":"Yanqiu Cui , Yaqi Tao , Tianjun Xu , Yuena Sun","doi":"10.1016/j.dci.2025.105537","DOIUrl":"10.1016/j.dci.2025.105537","url":null,"abstract":"<div><div>In recent years, the unique circular structure of circular RNAs (circRNAs) has been the focus of much attention and research. RNA molecules without coding function weave a complex regulatory network in mammals, affecting the progress of many biological processes. This discovery opens up a new perspective on the function of the genome. However, the powerful regulatory functions of circular RNAs in fish are still unclear. We identified a novel circular RNA, dubbing it circeIF4G3. Through qPCR and dual luciferase assays, we found that circeIF4G3 reduces the production of antiviral genes and inflammatory factors. We discovered that circeIF4G3 both hindered cell proliferation and diminished cell viability concurrently. These results suggest that circeIF4G3 is not only involved in the innate immunity of miiuy croaker, but also plays an inhibitory role in it. This discovery provides a new research approach for a deeper understanding of the immune regulatory mechanism of miiuy croaker. This study enriches the non-coding RNAs regulatory network in innate immunity in teleost fish, and establishes a foundation for investigating the role of circular RNAs in teleost fish's innate immunity.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"174 ","pages":"Article 105537"},"PeriodicalIF":2.4,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145687313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The VIP-VIPR2 axis strengthens intestinal barrier integrity and antimicrobial immunity in Ctenopharyngodon idella VIP-VIPR2轴增强海蛇肠道屏障完整性和抗菌免疫。

IF 2.4 3区农林科学

Developmental and comparative immunology Pub Date : 2026-01-01 Epub Date: 2025-12-16 DOI: 10.1016/j.dci.2025.105539

Xiaofeng Liu , Qian Hou , Zejun Zhou

{"title":"The VIP-VIPR2 axis strengthens intestinal barrier integrity and antimicrobial immunity in Ctenopharyngodon idella","authors":"Xiaofeng Liu , Qian Hou , Zejun Zhou","doi":"10.1016/j.dci.2025.105539","DOIUrl":"10.1016/j.dci.2025.105539","url":null,"abstract":"<div><div>Bacterial pathogens like <em>Aeromonas hydrophila</em> threaten aquaculture by compromising fish immune defenses. The intestinal barrier is a critical frontline defense against pathogenic bacteria in fish, yet its regulation by neuroimmune mechanisms remains poorly understood. In this study, we investigated the role of vasoactive intestinal peptide (VIP) in the immune response of grass carp (<em>Ctenopharyngodon idella</em>) following bacterial challenge. The full-length grass carp VIP gene was 462 bp, encoding a 153-amino acid precursor protein with high sequence conservation across vertebrates. VIP expression was highest in the intestinal tract and significantly upregulated upon <em>A. hydrophila</em> infection. Administration of recombinant grass carp VIP protein enhanced intestinal barrier function by increasing the expression of tight junction molecules (<em>ZO-1</em>, <em>Occludin</em>, <em>Claudin-1</em>) and the immunoregulatory cytokine IL-22, while reducing plasma LPS and D-lactate levels. Furthermore, grass carp VIP treatment promoted the expression of the mucosal barrier component <em>MUC2</em> and antimicrobial peptides (<em>LEAP-2</em>, <em>Lyz1</em>, <em>Hepcidin-1</em>), reduced bacterial load, and significantly improved survival rates. Mechanistic studies confirmed that VIP interacted specifically with the VIP receptor 2 (VIPR2), and knockdown of VIPR2 abolished the protective effects of VIP on barrier integrity and antimicrobial immunity. These findings demonstrate that the VIP-VIPR2 axis plays a crucial role in modulating intestinal immunity in grass carp, offering new insights into neuroimmune regulation in aquatic animals during pathogenic challenge.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"174 ","pages":"Article 105539"},"PeriodicalIF":2.4,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145780393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamic remodeling of gut microbiota and untargeted etabolomics in Sebastes schlegelii during Edwardsiella piscicida infection 鱼毒爱德华菌感染期间施莱格氏塞巴斯菌肠道菌群的动态重塑和非靶向代谢组学研究

IF 2.4 3区农林科学

Developmental and comparative immunology Pub Date : 2026-01-01 Epub Date: 2025-12-05 DOI: 10.1016/j.dci.2025.105534

Xiantong Liu , Ruixue Wu , Ning Ning Wang , Hua Xu , Xiaojun Rong , Chao Li , Min Cao

{"title":"Dynamic remodeling of gut microbiota and untargeted etabolomics in Sebastes schlegelii during Edwardsiella piscicida infection","authors":"Xiantong Liu , Ruixue Wu , Ning Ning Wang , Hua Xu , Xiaojun Rong , Chao Li , Min Cao","doi":"10.1016/j.dci.2025.105534","DOIUrl":"10.1016/j.dci.2025.105534","url":null,"abstract":"<div><div>The intestinal microbiota is a critical mediator of host immune responses and metabolic homeostasis, especially during the pathogenic challenge. This study investigated the dynamic remodeling of the gut microbiota and metabolome in <em>Sebastes schlegelii</em> following infection with the major aquaculture pathogen <em>Edwardsiella piscicida</em>. Using 16S rRNA gene sequencing and untargeted metabolomics, we analyzed the microbial community composition and metabolic profiles of intestinal samples at multiple time points post-infection. Our results revealed significant temporal shifts in bacterial diversity and structure. Notably, the abundances of genera such as <em>Bacteroides</em>, <em>Bacillus</em>, and <em>Lactobacillus</em> increased, while <em>Comamonas</em> and <em>Cutibacterium</em> decreased. Metabolomic analysis identified 1063 metabolites, with lipids and lipid-like molecules being the most abundant. Differential analysis revealed stage-specific metabolic alterations: early infection was marked by the upregulation of pro-inflammatory mediators such as lithocholic acid and palmitoylethanolamide, whereas late infection featured elevated levels of anti-inflammatory metabolites, including cholic acid and agmatine. KEGG pathway analysis indicated an initial enrichment in general metabolic processes, followed by a shift to steroid biosynthesis later in the infection. These findings suggest a coordinated “metabolic switch\" mechanism that modulates inflammation and promotes recovery. This study provides novel insights into the microbiota–metabolite–immune network in <em>S. schlegelii</em> and highlights potential biomarkers for monitoring fish health status in aquaculture.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"174 ","pages":"Article 105534"},"PeriodicalIF":2.4,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145696210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptomic analysis reveals the immune-related function of miR-122 in Larimichthys crocea 转录组学分析揭示了miR-122在鳜鱼中的免疫相关功能。

IF 2.4 3区农林科学

Developmental and comparative immunology Pub Date : 2026-01-01 Epub Date: 2025-12-23 DOI: 10.1016/j.dci.2025.105541

Lifang Wen , Sisi Wei , Li Wu , Zhenqi Xin , Mingshan Song , Weifeng Wang , Baoying Guo

引用次数: 0

Hypoxia impairs monocyte/macrophage function and host defense via ALAS2-mediated heme biosynthesis in Japanese sea bass (Lateolabrax japonicus) 缺氧通过alas2介导的日本黑鲈血红素生物合成损害单核/巨噬细胞功能和宿主防御

IF 2.4 3区农林科学

Developmental and comparative immunology Pub Date : 2026-01-01 Epub Date: 2025-12-15 DOI: 10.1016/j.dci.2025.105538

Wei-Wei Fang , Yi-Xin Wu , Yi-Bin Cao , Xin-Jiang Lu , Jian-Rao Hu

{"title":"Hypoxia impairs monocyte/macrophage function and host defense via ALAS2-mediated heme biosynthesis in Japanese sea bass (Lateolabrax japonicus)","authors":"Wei-Wei Fang , Yi-Xin Wu , Yi-Bin Cao , Xin-Jiang Lu , Jian-Rao Hu","doi":"10.1016/j.dci.2025.105538","DOIUrl":"10.1016/j.dci.2025.105538","url":null,"abstract":"<div><div>Hypoxia compromises host defense in fish by impairing macrophage function, but the underlying mechanisms remain unclear. This study elucidates the molecular mechanisms by which hypoxia disrupts monocytes/macrophages (MO/MФ) function in Japanese sea bass (<em>Lateolabrax japonicus</em>), with a particular focus on the regulatory role of heme biosynthesis and its key gene, <em>5′-aminolevulinate synthase 2</em> (<em>ALAS2</em>). Phylogenetic analysis confirmed high evolutionary conservation of <em>ALAS2</em> across vertebrates, supporting its fundamental role in heme metabolism. Transcriptomic analysis revealed that hypoxia induced the upregulation of genes involved in heme biosynthesis of MO/MФ, particularly <em>ALAS2</em>. Functional assays demonstrated that heme accumulation under hypoxic conditions impaired MO/MФ phagocytic and bactericidal activities, while also promoted the production of pro-inflammatory cytokines. These dysfunctions were mediated by the <em>ALAS2</em>-heme signaling axis, which orchestrated inflammatory responses and metabolic reprogramming throughout the study. Knockdown of <em>ALAS2</em> enhanced phagocytic activity and suppressed pro-inflammatory cytokine expression, suggesting its critical role in modulating MO/MФ function under hypoxia. Furthermore, inhibition of heme synthesis improved fish survival rates and reduced bacterial burdens during <em>Vibrio harveyi</em> infection. These findings elucidate the pivotal role of heme in regulating MO/MФ function and host defense under hypoxic conditions, providing mechanistic insights into hypoxia-induced immune suppression in aquaculture.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"174 ","pages":"Article 105538"},"PeriodicalIF":2.4,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145773879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptomic and lipidomic analysis provide insights into ovarian development of the mud crab Scylla paramamosain under high-temperature and strong illumination conditions 转录组学和脂质组学分析为高温强光条件下泥蟹Scylla paramamosain卵巢发育提供了新的思路。

IF 2.4 3区农林科学

Developmental and comparative immunology Pub Date : 2026-01-01 Epub Date: 2025-12-24 DOI: 10.1016/j.dci.2025.105542

Li Lu, Tao Wang, Xiaopeng Wang, Liangjie Liu, An Liu, Haihui Ye

{"title":"Transcriptomic and lipidomic analysis provide insights into ovarian development of the mud crab Scylla paramamosain under high-temperature and strong illumination conditions","authors":"Li Lu, Tao Wang, Xiaopeng Wang, Liangjie Liu, An Liu, Haihui Ye","doi":"10.1016/j.dci.2025.105542","DOIUrl":"10.1016/j.dci.2025.105542","url":null,"abstract":"<div><div>Environmental factors such as temperature, salinity, illumination intensity, and photoperiod exert profound influences on the physiology and reproduction of aquatic crustaceans. In this study, female mud crabs (<em>Scylla paramamosain</em>) were exposed to combined high-temperature and strong illumination stress (30 °C, 6000 lx) or maintained under control conditions (25 °C, 600 lx) for 30 days. To elucidate the impact of combined high-temperature and strong illumination stress on ovarian development, we conducted integrated analyses of transcriptomic data (RNA-Seq) from the hepatopancreas and ovary, alongside lipidomic profiles of the hepatopancreas and hemolymph. The hepatopancreas exhibited downregulation of lipid synthesis genes (e.g., <em>Fasn</em>, <em>SCD5</em>) and upregulation of lipid catabolism genes (e.g., <em>BBOX1</em>, <em>Pnlip</em>), which was associated with reduced lipid storage. Enrichment of sphingolipid signaling and autophagy pathways in hemolymph, along with elevated phosphatidylethanolamine species, indicated activation of protective mechanisms to maintain systemic balance. The ovary contained 1,457 differentially expressed genes, including upregulated stress-related genes (e.g., <em>Lrp1b</em>, <em>Tcab1</em>) and downregulated reproduction-related genes (e.g., <em>Igf1r</em>, <em>LENG9</em>), reflecting a trade-off between reproductive suppression and stress adaptation. In conclusion, these findings provide new insights into the adaptation of the mud crab to dual environmental stressors through coordinated molecular adjustments, offering valuable information for aquaculture management in response to sudden weather changes.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"174 ","pages":"Article 105542"},"PeriodicalIF":2.4,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145833314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study on the regulatory mechanism of apoptosis and expression patterns of key genes (p53, Bax, and Bcl-2) in the crustacean Eriocheir hepuensis under Microcystin-LR stress 微囊藻毒素- lr胁迫下合浦螯蟹凋亡调控机制及关键基因p53、Bax、Bcl-2表达模式的研究

IF 2.4 3区农林科学

Developmental and comparative immunology Pub Date : 2026-01-01 Epub Date: 2025-12-05 DOI: 10.1016/j.dci.2025.105532

Boyu Chen , Zihang Ma , Jinxia Liu , Lei Yan , Zhenyu Lan , Fanxi Gao , Zizhuo Liu , Shimiao Li , Zhenwen Zhang , Yan Zhang , Peng Zhu , Yongyan Liao

{"title":"Study on the regulatory mechanism of apoptosis and expression patterns of key genes (p53, Bax, and Bcl-2) in the crustacean Eriocheir hepuensis under Microcystin-LR stress","authors":"Boyu Chen , Zihang Ma , Jinxia Liu , Lei Yan , Zhenyu Lan , Fanxi Gao , Zizhuo Liu , Shimiao Li , Zhenwen Zhang , Yan Zhang , Peng Zhu , Yongyan Liao","doi":"10.1016/j.dci.2025.105532","DOIUrl":"10.1016/j.dci.2025.105532","url":null,"abstract":"<div><div>Microcystin-LR (MC-LR), a cyanobacterial toxin, poses a considerable threat to aquatic organisms. Herein, we focused on the economically important crustacean <em>Eriocheir hepuensis</em> to examine the acute toxic effects of MC-LR stress and its molecular mechanisms. The half-lethal concentration (LD<sub>50</sub>) of MC-LR for the <em>E</em>. <em>hepuensis</em> was determined to be 64.78 μg/kg, with a calculated safety concentration of 6.48 μg/kg through a 96-h acute toxicity test (n = 240). Exposure to a sublethal concentration (60 μg/kg) for 48 h resulted in 56.7 % survival rate (n = 60) and caused substantial hepatopancreatic pathological damage, including cell vacuolation, chromatin condensation, and mitochondrial fragmentation. To elucidate the underlying mechanisms, further cloning and identification of the apoptosis-related genes <em>p53</em> (open reading frame (ORF 1284 bp), <em>Bax</em> (ORF 1482 bp), and <em>Bcl-2</em> (ORF 732 bp) showed that their expression levels were the highest in the hepatopancreas (2.9, 3.7, and 3.5 times that of the control group, respectively). Time-series analysis further showed that the <em>p53</em> and <em>Bax</em> gene expression rapidly increased at stress onset but gradually declined, whereas the <em>Bcl-2</em> gene expression was temporarily downregulated, and a substantial negative correlation was observed with <em>Bax</em> expression. These molecular dynamics reveal that MC-LR-induced hepatopancreatic damage and mortality in <em>E. hepuensis</em> are mediated through coordinated apoptotic gene regulation, with the <em>p53</em>-<em>Bax</em>-<em>Bcl2</em> pathway orchestrating the cellular stress response. This study providing crucial scientific insights into the molecular responses of crustaceans to algal toxins.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"174 ","pages":"Article 105532"},"PeriodicalIF":2.4,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145699500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0