Developmental and comparative immunology最新文献

{"title":"Mx of Sebastes schlegelii: expression pattern, antibacterial activity and antiviral mechanism","authors":"Min Zhang ,&nbsp;Yue Wang ,&nbsp;Kai Yang ,&nbsp;Ziyue Chen ,&nbsp;Nuo Sun ,&nbsp;Guanghua Wang","doi":"10.1016/j.dci.2025.105374","DOIUrl":"10.1016/j.dci.2025.105374","url":null,"abstract":"<div><div>Myxovirus resistance (Mx) is a classical antiviral molecule that has been well understood in mammals. However, very limited studies on Mx antiviral activities have been documented in teleosts. In the present study, a novel Mx (SsMx) was cloned from black rockfish (<em>Sebastes schlegelii</em>) and the immunological activities of SsMx were examined <em>in vitro</em> and <em>in vivo</em>. SsMx contained conserved structural and functional domains including GTPase domain and GTPase effector domain. Quantitative real-time PCR (qRT-PCR) revealed that SsMx is extensively distributed in the immune cells and tissues examined with higher levels in spleen and liver. The mRNA expression of SsMx was significantly upregulated in head kidney, spleen and head kidney macrophages after pathogen infection. Recombinant SsMx (rSsMx) exhibited apparent binding activities against different bacteria <em>in vitro</em>. <em>In vivo</em> studies showed that rSsMx reduced pathogen dissemination and replication in head kidney and spleen. The subcellular localization results demonstrated that SsMx was predominantly distributed in the cytoplasm of transfected cells. Furthermore, SsMx was observed to inhibit apoptosis in virus-infected cells and reduce viral replication by interfering with the viral entry. SsMx and Spring viremia of carp virus Glycoprotein (SVCV G) were found to interact strongly with each other by co-immunoprecipitation and co-localization. These findings reveal an important role of SsMx in defencing the early stage of SVCV infection, which will be helpful to understand the molecular details of the antiviral mechanisms mediated by Mx proteins in teleosts.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"167 ","pages":"Article 105374"},"PeriodicalIF":2.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143886642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular characterization, expression, evolutionary analysis and molecular docking of the Janus activated kinase family members of largemouth bass (Micropterus salmoides)","authors":"Xiangyu Pi ,&nbsp;Mingzhu Pan ,&nbsp;Qihuan Zhang ,&nbsp;Zisheng Wang ,&nbsp;Zhitao Qi","doi":"10.1016/j.dci.2025.105375","DOIUrl":"10.1016/j.dci.2025.105375","url":null,"abstract":"<div><div>Janus kinases (JAKs) play crucial roles in the immune responses by binding the cytokine receptors. In the present study, five JAKs family members (JAK1, JAK2a, JAK2b, JAK3, and TYK2) were identified in largemouth bass (<em>Micropterus salmoides</em>). The five JAKs members of largemouth bass contained several conserved protein domains, including a FERM domain, an SH2 domain, a pseudokinase (STyrkc) domain, and a tyrosine kinase (Tyrkc) domain. Phylogenetic analysis revealed the JAK2/2a/2b and JAK3 formed a clade and the JAK1/TYK2 formed another separate clade. Realtime qPCR detection showed that all five JAKs genes were constitutively expressed in ten selected tissues, with highly expression in spleen and head kidney (HK). Following <em>Edwardsiella piscicida</em> infection, the five JAKs genes were significantly upregulated in the spleen and primary hepatocytes at different times post infection. Further selection pressure analysis revealed the five JAKs members underwent negative selection pressures during evolution. The FERM and Tyrkc domains of the five JAK members occurred purifying selection, and involved in interaction with STAT3, which confirmed by protein-protein interaction (PPI) network and molecular docking analysis. Also, molecular docking results indicated that the hydrogens bonds and salt bonds had crucial roles in the JAK/STAT3 complexes formation. Our results indicated that JAKs had important roles in the immune response against bacterial in largemouth bass, providing basis for elucidating the mechanism of JAK/STAT signaling pathway in fish.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"167 ","pages":"Article 105375"},"PeriodicalIF":2.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143916204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Type II interferons activate MHC-I pathway to enhance antigen presentation of grass carp reovirus VP35 DNA vaccine","authors":"Weihua Zhao ,&nbsp;Yueshuang Ji ,&nbsp;Yinghao Huang ,&nbsp;Yanwei Zhang ,&nbsp;Zhao Jia ,&nbsp;Kangyong Chen ,&nbsp;Wa Gao ,&nbsp;Gaoliang Yuan ,&nbsp;Jun Zou","doi":"10.1016/j.dci.2025.105384","DOIUrl":"10.1016/j.dci.2025.105384","url":null,"abstract":"<div><div>Hemorrhagic disease caused by grass carp reovirus (GCRV) poses a significant threat to the health and sustainability of grass carp (<em>Ctenopharyngodon idella</em>) farming. There are no effective measures to control the outbreaks of the disease. While DNA vaccines have proved to be promising to enhance the survival of vaccinated fish to GCRV infection, the protective efficacy is not maximized, and necessitates further improvement. This study explores the immunomodulatory potential of type II interferons (IFNs), including IFN-γ and IFN-γ related molecule (IFN-γrel), as adjuvants for GCRV-VP35 DNA vaccine. Expression plasmids, including pcDNA3.1-VP35, pcDNA3.1-IFN-γ, and/or pcDNA3.1-IFN-γrel, were intramuscularly administered in grass carp, and their effects on the expression of immune genes evaluated. Immunofluorescence microscopy confirmed the localized expression of GCRV-VP35, IFN-γ and IFN-γrel at the injection site, with the persistent expression detected for at least five weeks. Moreover, co-administration of IFN-γ and IFN-γrel plasmids synergistically enhanced the expression of <em>Mx1</em>, <em>Isg15</em> and <em>Viperin</em> to a greater extent than either plasmid alone. The <em>Igm</em> and <em>Cd8</em> genes were also upregulated in the spleen and muscle of fish injected with the IFN-γ/IFN-γrel plasmids. Furthermore, our findings reveal that IFN-γ and IFN-γrel robustly upregulated the expression of <em>Mhc I</em> but not <em>Mhc II</em> to promote antigen presentation of VP35 vaccine. The results indicate that type II IFNs have potential as adjuvants to enhance the immunogenicity and efficacy of DNA vaccines in protecting fish against viral infection.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"167 ","pages":"Article 105384"},"PeriodicalIF":2.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143913166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating transcriptomics and metabolomics revealed pathogenic mechanism of Chinese soft-shell turtle (Trionyx sinensis) infected with Trionyx sinensis hemorrhagic syndrome virus (TSHSV)","authors":"Weifeng Shen ,&nbsp;Shuang Zhao ,&nbsp;Sunjian Lyu ,&nbsp;Mingxing Zhang ,&nbsp;Qi Guo ,&nbsp;Bao Lou ,&nbsp;WenJun Ma ,&nbsp;Jingjing Zhan ,&nbsp;Li Liu ,&nbsp;Liang Li","doi":"10.1016/j.dci.2025.105373","DOIUrl":"10.1016/j.dci.2025.105373","url":null,"abstract":"<div><div><em>Trionyx sinensis</em> Hemorrhagic Syndrome Virus(TSHSV)seriously hinders the aquaculture of Chinese soft-shell turtle (<em>Trionyx sinensis</em>) due to its high mortality. However, the pathogenic mechanisms of TSHSV in <em>T. sinensis</em> are still unclear. In present study, transcriptomic and metabolomic analyses were performed on turtle livers following TSHSV infection. 734 up-regulated and 770 down-regulated differentially expressed genes (DEGs) were identified in different TSHSV challenge groups. These DEGs were categorized into 12 pathways related to virus infection and host immunity. Moreover, 27, 2679, and 4341 differentially expressed metabolites (DEMs) were identified in the D1, D3, and D5 groups, respectively. These DEMs were mapped into the pathways of energy metabolism, amino acid metabolism and fatty acid metabolism. Association analysis revealed TSHSV induced inflammatory responses, hepatocyte apoptosis, and ultimately led to liver tissue damage. Taurine supplementation promoted the survival rate of turtle after TSHSV infection and reduced the inflammatory response of liver by regulating the production of interferons, antioxidases, and the pro-inflammatory cytokine TNF-α. Collectively, our results provide comprehensive profiles of the transcriptome and metabolome in Chinese soft-shell turtle liver after TSHSV invasion, shedding light on the underlying pathogenic mechanism. The method of taurine supplementation might be a promising therapeutic strategy for protecting turtles from TSHSV.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"166 ","pages":"Article 105373"},"PeriodicalIF":2.7,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143854839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The class A scavenger receptor member 3 (SCARA3) regulates cell apoptosis through X-linked apoptosis inhibitory protein (XIAP) in turbot (Scophthalmus maximus L.)","authors":"Xinghua Zhuang ,&nbsp;Xuan Chen ,&nbsp;Lili Cao ,&nbsp;Beibei Wang ,&nbsp;Zhongyi Wang ,&nbsp;Suwan Li ,&nbsp;Honghong Li ,&nbsp;Chao Li ,&nbsp;Ning Yang","doi":"10.1016/j.dci.2025.105370","DOIUrl":"10.1016/j.dci.2025.105370","url":null,"abstract":"<div><div>Class A scavenger receptor 3 (SCARA3), a macrophage scavenger receptor-like protein, plays important roles in inhibiting cell proliferation, migration, and invasion. In the present study, a SCARA3 gene of turbot (<em>Sm</em>SCARA3) (Gene ID: 118289953) with an 1815 bp ORF encoding 604 amino acids was identified. Phylogenetic analysis revealed that <em>Sm</em>SCARA3 showed the closest relationship to that counterpart of olive flounder (<em>Paralichthys olivaceus</em>). The synteny analysis demonstrated conserved syntenic patterns across selected vertebrates. In addition, <em>Sm</em>SCARA3 was ubiquitously expressed in all the examined tissues, with the highest expression level in intestine and the lowest expression level in the brain. <em>Sm</em>SCARA3 exhibited different expression patterns in mucosal tissues (intestine, gill, skin) after two bacterial infections. Subsequently, recombinant <em>Sm</em>SCARA3 protein (r<em>Sm</em>SCARA3) revealed the strong binding affinity to LPS and responded primarily to LPS stimulation in intestinal cells of turbot. Additionally, the interference and overexpression experiments indicated that <em>Sm</em>SCARA3 was associated with apoptosis related genes, such as Caspase1, Caspase3 and Caspase3a, and it could activate Caspase3 in HEPG2 cells. Moreover, flow cytometry revealed the apoptosis of <em>Sm</em>SCARA3 overexpression group increased by 10.03%, which was consistent with the effect of <em>Sm</em>SCARA3 on proliferation inhibition in intestinal cells of turbot. The cell apoptosis levels in the <em>Sm</em>SCARA3-Flag and XIAP-HA experimental group were significantly lower than that in the control group (51.17% vs 72.72%). Finally, the Co-IP assay showed that <em>Sm</em>SCARA3 could directly interact with XIAP. In conclusion, our results indicated that <em>Sm</em>SCARA3 could activate Caspase3 and modulate apoptosis through XIAP , highlighting its potential roles as a therapeutic target for fish diseases.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"166 ","pages":"Article 105370"},"PeriodicalIF":2.7,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143802725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EXOC8 of Epinephelus coioides involved in SGIV infection via innate immunity and apoptosis","authors":"Wei Huang ,&nbsp;Zi-An Chen ,&nbsp;Qi-Yin Li ,&nbsp;Cui-Fen Huang ,&nbsp;Yun-Xiang Lin ,&nbsp;Yin-Mei Lan ,&nbsp;Ze-Peng Zhang ,&nbsp;Yu-Feng Jiang ,&nbsp;Qi-Wei Qin ,&nbsp;Hong-Yan Sun","doi":"10.1016/j.dci.2025.105368","DOIUrl":"10.1016/j.dci.2025.105368","url":null,"abstract":"<div><div>The exocyst complex (EXOC) plays a major role in the extracellular secretion of organisms. In this study, EXOC8, a member of the EXOC family, was characterized from <em>Epinephelus coioides</em>,an important economical important fish in southern China and Southeast Asia, and its role response to viral infection was explored. The full length of <em>E</em>. <em>coioides</em> EXOC8 is 3091 bp including a 2061 bp open reading frame (ORF) encoding 686 amino acids, with a molecular mass of 79037.42 Da. The mRNA of <em>E</em>. <em>coioides</em> EXOC8 can be detected in all of the tissues examined with different levels. <em>E</em>. <em>coioides</em> EXOC8 is distributed in the cytoplasm. The expression of <em>E</em>. <em>coioides</em> EXOC8 was up-regulated during Singapore grouper iridovirus (SGIV) infection, an important pathogen of <em>E</em>. <em>coioides</em>. Overexpressing <em>E</em>. <em>coioides</em> EXOC8 significantly promoted the formation of cytopathic effects (CPE) caused by SGIV infection and the expressions of SGIV key genes MCP, VP19, LITAF and ICP18; but significantly inhibited the activities of NF-κB/AP-1 promoter, apoptosis induced by SGIV, and the expressions of inflammatory factors (IL-6,IL-8, IL-1<em>β</em> and TNF-α) in <em>E. coioides</em>. The results demonstrated that <em>E. coioides</em> EXOC8 may be involved in SGIV infection, providing a theoretical basis for clearing the mechanisms of viral infection in fish.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"166 ","pages":"Article 105368"},"PeriodicalIF":2.7,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143794548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ToIκB and ToIKK genes from golden pompano (Trachinotus ovatus): Molecular characterization, expression, and association with tolerance to Streptococcus agalactiae infection","authors":"Jie Gao ,&nbsp;Ming-Jian Liu ,&nbsp;Jin-Min Pan ,&nbsp;Hua-Yang Guo ,&nbsp;Bao-Suo Liu ,&nbsp;Ke-Cheng Zhu ,&nbsp;Nan Zhang ,&nbsp;Dian-Chang Zhang","doi":"10.1016/j.dci.2025.105369","DOIUrl":"10.1016/j.dci.2025.105369","url":null,"abstract":"<div><div>Effective disease management is crucial for sustainable aquaculture, particularly for economically important species like golden pompano (<em>Trachinotus ovatus</em>). <em>Streptococcus agalactiae</em> represents a major threat to this species, leading to severe health issues and significant economic losses. Understanding the immune mechanisms involved is essential to address this challenge. The <em>IκB</em> and <em>IKK</em> genes are known to be key regulators of immune responses, playing pivotal roles in modulating inflammatory pathways during infections. However, their specific roles in golden pompano immunity are not well characterized. In this study, we used bioinformatics analysis and tissue-specific expression profiling to investigate the roles of <em>IκB</em> and <em>IKK</em> genes in golden pompano during bacterial infection. The results demonstrated that <em>ToIKK</em> was significantly upregulated during the early stages of infection, indicating rapid immune activation, while <em>ToIκB</em> showed an initial decrease followed by recovery, suggesting its involvement in inflammation modulation. These genes were found to regulate the NF-κB signaling pathway, which is crucial for coordinating the immune response to bacterial infection. This research provides valuable insights into the molecular basis of golden pompano immune response against <em>S. agalactiae</em>, offering a foundation for developing targeted anti-infection strategies and improving disease resistance and health management practices in aquaculture.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"166 ","pages":"Article 105369"},"PeriodicalIF":2.7,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143786024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The molecular mechanism of ferroptosis in the Pacific oyster Crassostrea gigas under Erastin treatment or high temperature stress","authors":"Jinyuan Leng ,&nbsp;Jiejie Sun ,&nbsp;Zhicheng Guo ,&nbsp;Lingling Wang ,&nbsp;Linsheng Song","doi":"10.1016/j.dci.2025.105366","DOIUrl":"10.1016/j.dci.2025.105366","url":null,"abstract":"<div><div>Ferroptosis is an iron- and lipotoxicity-dependent form of programmed cell death, and it is distinct from apoptosis, pyroptosis, and autophagy. In the present study, the hemocytes were found to be shrunken under Erastin treatment or high temperature stress. The mitochondrial atrophy, crest loss and fracture were observed in hemocytes under high temperature stress. In addition, the fluorescence intensity of mitochondrial probe JC-1 monomers increased significantly in hemocytes under high temperature stress. Hemocytes were found to be wrinkled under ultrastructure and the contents of LPO, ROS and GSH increased significantly under Erastin treatment or high temperature stress. The band intensity of <em>Cg</em>VDAC2 also decreased under Erastin treatment or high temperature stress. The mRNA expressions of genes involved in enhancing the antioxidation system as well as genes involved in promoting the iron metabolism all decreased significantly under Erastin treatment or high temperature stress. Those of genes involved in impairing the antioxidation system, genes involved in inhibiting the iron metabolism, as well as genes involved in reducing the lipid peroxidation all increased significantly under Erastin treatment or high temperature stress. These results indicated that Erastin could activate the three key ferroptotic signaling pathways in oyster and the activation mechanism of ferroptosis in oyster under high temperature stress was similar with that under Erastin treatment.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"166 ","pages":"Article 105366"},"PeriodicalIF":2.7,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143788026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FK506 activates the BMP signaling pathway to regulate ovarian development in Portunus trituberculatus","authors":"Xiaocong Chen ,&nbsp;Ce Shi ,&nbsp;Yangfang Ye ,&nbsp;Chunlin Wang ,&nbsp;Ronghua Li ,&nbsp;Huan Wang ,&nbsp;Congcong Hou ,&nbsp;Weiwei Song ,&nbsp;Changkao Mu","doi":"10.1016/j.dci.2025.105365","DOIUrl":"10.1016/j.dci.2025.105365","url":null,"abstract":"<div><div>Bone morphogenic proteins (BMPs) play important regulatory roles in the development of follicles in mammals. However, studies on the roles of BMPs in ovarian development in low-level aquatic animals, especially the swimming crab <em>Portunus trituberculatus</em>, are limited. In this study, a BMP Ⅰ-type receptor-specific activator (tacrolimus, FK506) was administered at different concentrations via in vivo injection, and the effects of FK506 on the regulation of the BMP signaling pathway during ovarian development in <em>P</em>. <em>trituberculatus</em> were examined. The tissue and cell morphology was observed, and a combined transcriptomics, proteomics and metabolomics analysis was carried out. Crabs administered FK506 exhibited elevated GSI alongside reduced HSI compared to control and blank groups. The main biological processes enriched by joint analysis included lipid metabolism, sugar metabolism, and amino acid metabolism. Fatty acid composition analysis revealed that the activator may activate the BMP signaling pathway to promote ovarian development and accelerate the transport of unsaturated fatty acids from the hepatopancreas to the ovaries. Amino acid metabolism and carbohydrate metabolism provide transporter proteins and energy for lipid metabolism. This study is highly important because it reveals the molecular mechanism by which the BMP signaling pathway regulates gonadal development in a crustacean.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"166 ","pages":"Article 105365"},"PeriodicalIF":2.7,"publicationDate":"2025-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143763309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A preliminary study on the regulation and impact of unfolded protein response on circadian genes in Litopenaeus vannamei","authors":"Wan-Yi Chen ,&nbsp;Ze-Yu Yan ,&nbsp;Shu-Chen ,&nbsp;An-Na Liu ,&nbsp;Yu-Tao Miao ,&nbsp;Lei Wang ,&nbsp;Yi-Hong Chen","doi":"10.1016/j.dci.2025.105364","DOIUrl":"10.1016/j.dci.2025.105364","url":null,"abstract":"<div><div>Circadian rhythm serves as an adaptive mechanism to environmental changes and is known to regulate multiple physiological processes, such immune response and metabolism. This study explores how unfolded protein response (UPR) affects circadian rhythm genes and their implications at immune and metabolic systems in <em>Litopenaeus vannamei</em>. Under 12 light-dark cycle conditions, <em>Cry1</em> (<em>LvCry1</em>), <em>Period</em> (<em>LvPeriod</em>), and <em>Vrille</em> (<em>LvVrille</em>) had the highest expression in the eyestalk, while <em>Timeless</em> (<em>LvTimeless</em>) and <em>Shaggy</em> (<em>LvShaggy</em>) were most expressed in the hepatopancreas and nerve. Tunicamycin (TM) injection activated the shrimp UPR and affect the expression of circadian rhythm genes. The time of expression peak of <em>LvCry1</em> getting earlier, while <em>LvPeriod</em> or <em>LvTimeless</em> peak phase is left shift; <em>LvVrille</em> expression appears circadian disorder, the expression level decreases and the amplitude is significantly lower than the control group. Further investigation revealed that PERK-eIF2α pathway of UPR system may be crucial for its influence on the circadian rhythm gene expression. Further investigation showed that knocked-down expression of <em>LvTimeless</em>, which was significantly affected by the UPR, combined with transcriptomic analysis uncovered that some metabolic and immune-related genes was significantly affected by circadian rhythm. These results have laid a foundation for us to continue to deeply understand the relationship between environmental stress, circadian rhythm and health in <em>L. vannamei.</em></div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"166 ","pages":"Article 105364"},"PeriodicalIF":2.7,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143742632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}