Developmental and comparative immunology最新文献

{"title":"Allogenic grafting induces PI3K-mediated tissue overgrowth in hydrozoan jellyfish Cladonema radiatum medusae.","authors":"Crystal Tang, Miwa Tamura-Nakano, Kazunori Tachibana","doi":"10.1016/j.dci.2025.105367","DOIUrl":"10.1016/j.dci.2025.105367","url":null,"abstract":"<p><p>Allorecognition, which is the ability of an organism to discriminate between self and non-self, protects multicellular animals from somatic cell/germline parasitism. We reported and characterised allorecognition in the stolons of colonies of the hydrozoan jellyfish Cladonema radiatum (C. radiatum) in our previous publication. C. radiatum has a free-swimming medusa form besides the sessile colonial form. In this study, we investigated the allorecognition responses in the medusa form of C. radiatum. By using grafting experiments, we observed that while C. radiatum medusae show tolerance to both isogenic and allogenic chimerism, allogenic grafting induces the formation of a form of circular scars-we refer to as \"ring-shaped scars\"-around the grafts on the host umbrella. Within the scars, overgrowth of tissues occurs with additional gastrovascular canal development. By pharmaceutical experiments, we found that tissue overgrowth is dependent on phosphoinositide 3-kinase (PI3K) and vascular endothelial growth factor (VEGF), showing a resemblance to mammalian neoplasia.</p>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":" ","pages":"105367"},"PeriodicalIF":2.7,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143788022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FK506 activates the BMP signaling pathway to regulate ovarian development in Portunus trituberculatus","authors":"Xiaocong Chen ,&nbsp;Ce Shi ,&nbsp;Yangfang Ye ,&nbsp;Chunlin Wang ,&nbsp;Ronghua Li ,&nbsp;Huan Wang ,&nbsp;Congcong Hou ,&nbsp;Weiwei Song ,&nbsp;Changkao Mu","doi":"10.1016/j.dci.2025.105365","DOIUrl":"10.1016/j.dci.2025.105365","url":null,"abstract":"<div><div>Bone morphogenic proteins (BMPs) play important regulatory roles in the development of follicles in mammals. However, studies on the roles of BMPs in ovarian development in low-level aquatic animals, especially the swimming crab <em>Portunus trituberculatus</em>, are limited. In this study, a BMP Ⅰ-type receptor-specific activator (tacrolimus, FK506) was administered at different concentrations via in vivo injection, and the effects of FK506 on the regulation of the BMP signaling pathway during ovarian development in <em>P</em>. <em>trituberculatus</em> were examined. The tissue and cell morphology was observed, and a combined transcriptomics, proteomics and metabolomics analysis was carried out. Crabs administered FK506 exhibited elevated GSI alongside reduced HSI compared to control and blank groups. The main biological processes enriched by joint analysis included lipid metabolism, sugar metabolism, and amino acid metabolism. Fatty acid composition analysis revealed that the activator may activate the BMP signaling pathway to promote ovarian development and accelerate the transport of unsaturated fatty acids from the hepatopancreas to the ovaries. Amino acid metabolism and carbohydrate metabolism provide transporter proteins and energy for lipid metabolism. This study is highly important because it reveals the molecular mechanism by which the BMP signaling pathway regulates gonadal development in a crustacean.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"166 ","pages":"Article 105365"},"PeriodicalIF":2.7,"publicationDate":"2025-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143763309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A preliminary study on the regulation and impact of unfolded protein response on circadian genes in Litopenaeus vannamei","authors":"Wan-Yi Chen ,&nbsp;Ze-Yu Yan ,&nbsp;Shu-Chen ,&nbsp;An-Na Liu ,&nbsp;Yu-Tao Miao ,&nbsp;Lei Wang ,&nbsp;Yi-Hong Chen","doi":"10.1016/j.dci.2025.105364","DOIUrl":"10.1016/j.dci.2025.105364","url":null,"abstract":"<div><div>Circadian rhythm serves as an adaptive mechanism to environmental changes and is known to regulate multiple physiological processes, such immune response and metabolism. This study explores how unfolded protein response (UPR) affects circadian rhythm genes and their implications at immune and metabolic systems in <em>Litopenaeus vannamei</em>. Under 12 light-dark cycle conditions, <em>Cry1</em> (<em>LvCry1</em>), <em>Period</em> (<em>LvPeriod</em>), and <em>Vrille</em> (<em>LvVrille</em>) had the highest expression in the eyestalk, while <em>Timeless</em> (<em>LvTimeless</em>) and <em>Shaggy</em> (<em>LvShaggy</em>) were most expressed in the hepatopancreas and nerve. Tunicamycin (TM) injection activated the shrimp UPR and affect the expression of circadian rhythm genes. The time of expression peak of <em>LvCry1</em> getting earlier, while <em>LvPeriod</em> or <em>LvTimeless</em> peak phase is left shift; <em>LvVrille</em> expression appears circadian disorder, the expression level decreases and the amplitude is significantly lower than the control group. Further investigation revealed that PERK-eIF2α pathway of UPR system may be crucial for its influence on the circadian rhythm gene expression. Further investigation showed that knocked-down expression of <em>LvTimeless</em>, which was significantly affected by the UPR, combined with transcriptomic analysis uncovered that some metabolic and immune-related genes was significantly affected by circadian rhythm. These results have laid a foundation for us to continue to deeply understand the relationship between environmental stress, circadian rhythm and health in <em>L. vannamei.</em></div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"166 ","pages":"Article 105364"},"PeriodicalIF":2.7,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143742632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring diversity in avian immune defence: Insights from cathelicidin clusters","authors":"Sergio González-Acosta ,&nbsp;Victoria Baca-González ,&nbsp;Patricia Asensio-Calavia ,&nbsp;Andrea Otazo-Pérez ,&nbsp;Manuel R. López ,&nbsp;Antonio Morales-delaNuez ,&nbsp;José Manuel Pérez de la Lastra","doi":"10.1016/j.dci.2025.105363","DOIUrl":"10.1016/j.dci.2025.105363","url":null,"abstract":"<div><div>Cathelicidins are a family of proteins from which a class of Host Defence Peptides (HDPs) is derived. They are components of the innate immune system of most vertebrates, including birds. Despite their promising activities, the genomic organisation and interspecies diversity of avian cathelicidins has been less studied than in other animal groups. In this research, we investigated the cathelicidin cluster in 72 avian species from 26 different orders by mining the avian genome assemblies available in NCBI database, using bioinformatics tools to analyse the cluster composition, gene structure and phylogenetic relationships. Cathelicidin clusters were found principally on chromosomes 1 and 2, usually located at the ends of the chromosomes, except in Falconiformes and Psittaciformes. The Galloanserae cluster diverged from the rest of avian groups by having cath1 in the Galliformes and a putative pseudogene of cathB1 in Anseriformes. In contrast, the remaining avian species displayed a predominantly cathelicidin cluster comprising cathB1, cath3, and cath2. However, Passeriformes lacked cath3 while Falconiformes exhibited the pseudogenisation of cath3. In addition, we found kelch like family member 18 and transforming growth factor beta 4 (zinc finger protein 777 in Passeriformes) as flanking genes. We identified 190 putative cathelicidins genes, of which 103 were undescribed, that displayed a high percentage of identity across cathelicidin type. Phylogenetic analysis revealed that cathelicidin genes are highly conserved supporting the hypothesis that cathelicidins play a crucial role in avian immunity. This work highlights the use of bioinformatic tools to improve our understanding of avian cathelicidins and the evolution of this important family protein.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"166 ","pages":"Article 105363"},"PeriodicalIF":2.7,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143699977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chitosan-selenium nanoparticles suppress infectious spleen and kidney necrosis virus through immune modulation and viral replication inhibition in zebrafish (Danio rerio)","authors":"Yi-Seol Jeong,&nbsp;Eun-Jin Baek,&nbsp;Kwang-Il Kim","doi":"10.1016/j.dci.2025.105362","DOIUrl":"10.1016/j.dci.2025.105362","url":null,"abstract":"<div><div>Infectious spleen and kidney necrosis virus (ISKNV) is one of the most challenging diseases causing substantial financial losses in global aquaculture. However, effective strategies for controlling ISKNV infections in ornamental fish are lacking. In this study, chitosan-selenium nanoparticles (CTS-SeNPs) were synthesized and evaluated as potential strategies for controlling ISKNV infection. The characterization of CTS-SeNPs confirmed the conjugation of chitosan with SeNPs, resulting in the formation of spherical particles with an average size of 51.73 nm and exhibiting moderate stability. Furthermore, the morphology and stability were retained for 24 h in the freshwater environment. The CTS-SeNPs exhibited viral suppression efficacy at 40 μg/mL, downregulating major capsid protein gene expression in ISKNV-infected dwarf gourami fin (DGF) cells. The inhibition rates at 48 and 72 hpi in the DGF cells were 94.02 ± 0.39 % and 91.82 ± 3.84 %, respectively. During the ISKNV replication cycle, CTS-SeNPs were found to affect the attachment and replication stage. In zebrafish, the viral suppression efficacy of CTS-SeNPs was evaluated by two different administration methods: intraperitoneal injection (IP) and immersion administration (IM). At safe concentrations (1 μg/mL for IP and 40 μg/mL for IM), CTS-SeNPs demonstrated improved survival rates of 53.33 ± 9.42 % (IP) and 50.00 ± 8.16 % (IM). Additionally, viral loads in survivors were lower than those in ISKNV-infected fish. In terms of immune gene expression, CTS-SeNPs upregulated interferon-inducible genes. Moreover, after ISKNV infection following CTS-SeNPs administration, type I interferon induction (interferon φ2 and interferon φ3) was identified, suggesting that CTS-SeNPs enhance viral suppression through immune modulation, indicating their potential as effective anti-ISKNV agents.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"166 ","pages":"Article 105362"},"PeriodicalIF":2.7,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploration of the differential expression patterns of immunoglobulin heavy chain genes in horses and donkeys","authors":"Yanbo Qiu ,&nbsp;Junyi Jiang ,&nbsp;Xiaohua Yi ,&nbsp;Shuhui Wang ,&nbsp;Xiuzhu Sun","doi":"10.1016/j.dci.2025.105360","DOIUrl":"10.1016/j.dci.2025.105360","url":null,"abstract":"<div><div>This study investigated the immunoglobulins (IG) gene locus structure and expression diversity in local Chinese horse and donkey breeds, including Ningqiang, Guanzhong horses, and varieties such as Guanzhong, Jiami, and Northern Shaanxi donkey using genome alignment and high-throughput sequencing. The aim was to expand understanding of IG expression patterns in horses, donkeys, and their different breeds. The results revealed that the donkey IGH locus contains 117 VH genes (23 functional), 44 DH genes, and 8 JH genes, arranged in a VH-DH-JH sequence on chromosome 7, spanning approximately 1189 kb. Both horses and donkeys exhibited high frequencies of the IGHV4-IGHD2-IGHJ6, IGHV4-IGHD4-IGHJ6, and IGHV4-IGHD2-IGHJ4 combinations during VDJ recombination. Significant differences in V, D, and J junctions between horses and donkeys, as well as among breeds, were observed, mainly due to variations in N-nucleotide insertion length. The CDR3H region in horses exhibited greater length diversity and a higher Cys content than that in donkeys, which may contribute to species-specific differences in IGH spatial structure. Both horses and donkeys showed a clear preference for A &gt; G and G &gt; A mutations during somatic hypermutation (SHM), with consistent trends across breeds and species. In conclusion, this study reveals that V(D)J recombination, junction diversity, and SHM are key mechanisms driving IGH diversity in horses and donkeys. While the basic mechanisms for IGH diversification are similar across species and breeds, their specific manifestations exhibit both distinct and consistent patterns, reflecting differences in immune system adaptations and providing a theoretical basis for understanding IGH expression diversity in equids.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"166 ","pages":"Article 105360"},"PeriodicalIF":2.7,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143669204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An integrated investigation of major environmental stressors on the Pacific white shrimp Litopenaeus vannamei","authors":"Lulu Han ,&nbsp;Jinyu Yang ,&nbsp;Peiyu Yan ,&nbsp;Mengqiang Wang","doi":"10.1016/j.dci.2025.105361","DOIUrl":"10.1016/j.dci.2025.105361","url":null,"abstract":"<div><div>Ammonia, nitrite and sulfide are major environmental stressors in aquaculture. They can injury tissue structure, interfere with the immune system, impair growth and development, and make aquatic animals more vulnerable to infections. In the present study, four groups were set up as Control group (no treatment), Ammonia group (30 mg/L ammonia-N), Nitrite group (60 mg/L nitrite-N) and Sulfide group (4 mg/L sulfide), so as to examine the effects of different environmental stressors on histopathology, immune indices and transcriptome of <em>Litopenaeus vannamei</em>. After 48 h stimulation, the results showed that all the three environmental stressors caused damage to the hepatopancreas, midgut, muscle and gill tissues of the shrimp, and that the hepatopancreas and midgut were the most seriously damaged and the muscle was the least affected. Superoxide dismutase (SOD) activity increased and total antioxidant capacity (T-AOC) activity decreased in all the three treatment groups. Glutathione peroxidase (GSH-PX) activity decreased significantly in Sulfide group and alkaline phosphatase (AKP) activity increased significantly in Ammonia and Nitrite groups. In the hepatopancreas of the Ammonia, Nitrite, and Sulfide groups, transcriptome analysis revealed 709, 715 and 289 differential expressed gene (DEGs), respectively. GO and KEGG enrichment demonstrated that the three environmental stressors had an impact on the shrimp's immune response, metabolic processes, growth and development. The combination of histopathology, immune indicators, and transcriptome yielded that the Nitrite group had the most serious impact on the shrimp, followed by Ammonia group, while Sulfide group had the least impact.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"166 ","pages":"Article 105361"},"PeriodicalIF":2.7,"publicationDate":"2025-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143644945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of two immunoglobulin light chain types and expression of immunoglobulin diversity in Chinese giant salamander (Andrias davidianus)","authors":"Xiangyu Liu ,&nbsp;Xiuzhu Sun ,&nbsp;Huyang Bao ,&nbsp;Zhanjun Ren ,&nbsp;Shuhui Wang","doi":"10.1016/j.dci.2025.105358","DOIUrl":"10.1016/j.dci.2025.105358","url":null,"abstract":"<div><div>Lacking research on immunoglobulins in the Chinese giant salamanders (<em>Andrias davidianus</em>) has left their populations vulnerable to pathogen infections, contributing to a sharp decline in their numbers. In this study, we employed the rapid amplification of cDNA ends (RACE) technique along with paired-end 300 bp read length (PE300) sequencing. This approach was used to construct a DNA library, which enabled us to investigate the diversity of immunoglobulin gene expression. Through this approach, we identified structural features of immunoglobulin light chains. Our results revealed the presence of Ig<em>λ</em> and Ig<em>σ</em>. Similar to other vertebrates, the immunoglobulin light chains of Chinese giant salamanders are composed of variable (V) and constant (C) domains connected by recombination activating gene (RAG) mediated V-J (joining) recombination. This canonical gene organization allows combinatorial diversity through rearrangement of multiple V and J gene segments. The <em>IgLC</em> features FPPS and FYP motifs, showing high similarity to both mammalian <em>IgLC</em> sequences and the <em>IgLC</em> of the Chinese Alligator <em>(Alligator sinensis)</em>. The <em>IgSC,</em> characterized by SSYL structures, showed strong homology with fish and amphibian <em>IgSC</em> sequences, notably the axolotl <em>(Ambystoma mexicanum) IgSC</em>. Both the <em>IgLV</em> and <em>IgSV</em> sequences exhibit a YYCXX fold in the last five residues of framework region 3 (FR3). FR3 is a critical framework region within the V domain that anchors the antigen binding complementarity determining regions. Notably, the FPPS/FYP motifs in <em>Igλ</em> and SSYL motifs in <em>Igσ</em> exhibited evolutionary conservation patterns consistent with those in other vertebrates. In terms of gene expression diversity, the <em>IgH</em> is composed of 7 <em>IgHV</em>, 7 <em>IgHD</em>, and 6 <em>IgHJ</em> subgroups, while the <em>Igλ</em> consists of 10 <em>IgLV</em> and 7 <em>IgLJ</em> subgroups, and the <em>Igσ</em> comprises 5 <em>IgSV</em> and 7 <em>IgSJ</em> subgroups. Dominant <em>IgH</em> combinations are <em>IgHV4-IgHD3-IgHJ4</em> and <em>IgHV4-IgHD2-IgHJ4</em>. The <em>Igλ</em> shows high usage of <em>IgLV8</em>, <em>IgLV3</em>, <em>IgLJ7</em>, and <em>IgLJ3</em>, while the <em>Igσ</em> is predominantly characterized by <em>IgSV3-IgSJ3</em>. Notably, Cys utilization in the complementarity determining region 3 (CDR3) region was extremely low, suggesting that gene conversion plays a significant role in immune adaptation. This research enriches the immune genetic map of the Chinese giant salamanders and enhances our understanding of immunoglobulin evolution in tetrapods.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"166 ","pages":"Article 105358"},"PeriodicalIF":2.7,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143639493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of a target polypeptide of the CD169 receptor of bovine macrophage using a phage display peptide library","authors":"Chunyu Tong ,&nbsp;Qi Liu ,&nbsp;Zhelin Zhang ,&nbsp;Yimin Liang,&nbsp;Wenzhi Feng,&nbsp;Honghao Yu,&nbsp;Di Lan,&nbsp;Qianyi Liu,&nbsp;Bocui Song","doi":"10.1016/j.dci.2025.105359","DOIUrl":"10.1016/j.dci.2025.105359","url":null,"abstract":"<div><div>CD169, a salivary acid adhesion receptor on macrophages, plays a crucial role in enhancing the phagocytic response to pathogenic bacteria and in antibacterial immunity. To explore its potential in targeted veterinary drug applications, we used phage display technology to biopan peptide fragments specific to CD169. After several rounds of screening, 45 phage clones were selected for ELISA testing, resulting in 21 high-affinity clones. DNA sequencing revealed that 65 % of the peptides shared a common amino acid sequence (APRL∗∗∗HHH). A 12-amino acid peptide, CD169-T1, was synthesized with rhodamine B labeling at the N-terminal to assess its targeting capability. Flow cytometry and immunofluorescence assays confirmed that CD169-T1 specifically binds to HEK293T cells expressing bovine CD169 and murine macrophages, showing red fluorescence at the cell membrane. Moreover, the fluorescent marker of CD169-T1 was detected in the flow cytometry test results, thus confirming that the CD169-T1 has a significant targeting effect. The CD169-T1 obtained in this study can serve as the targeted part of the immune preparations to prevent and treat a wide variety of pathogenic bacteria, thus significantly facilitating the antigen presentation and increasing the utilization rate and the immune protection effect. Furthermore, this study provides a reference for the targeted research on CD169.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"166 ","pages":"Article 105359"},"PeriodicalIF":2.7,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143628679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characteristics of different scuticociliates from an infected olive flounder (Pararlichthys olivaceus) farm in South Korea","authors":"Junewoo Park ,&nbsp;Kyun-Woo Lee ,&nbsp;Yeun Park ,&nbsp;Jeong Hyeon Yeo ,&nbsp;Jae-Ho Jung ,&nbsp;Hye-Min Kang","doi":"10.1016/j.dci.2025.105357","DOIUrl":"10.1016/j.dci.2025.105357","url":null,"abstract":"<div><div>Parasitic diseases are considered to be a major problem in olive flounder (<em>Paralichthys olivaceus</em>) farms in South Korea, with scuticociliate infections being especially problematic due to their year-round occurrence and difficulty in control. In this study, we collected scuticociliates from flounder showing typical symptoms of scuticociliasis, such as ulcers, hemorrhages, and necrosis, at farms in Busan and Jeju, South Korea. Four species of scuticociliates were identified: <em>Mesanophrys carcini</em>, <em>Miamiensis avidus</em>, <em>Paralembus digitiformis</em>, and <em>Pseudocohnilembus persalinus</em>, the <em>M. carcini</em> and <em>P. digitiformis</em> being recorded for the first time in this study. Pathogenicity tests on healthy and wounded fish revealed that <em>M. avidus</em> was highly lethal, while the other species had minimal effects on healthy fish. However, <em>P. digitiformis</em> and <em>P. persalinus</em> impeded wound healing and showed high infection rates in wounded fish without causing high mortality. These two species also triggered early up-regulation of stress-related genes, such as complement C3, followed by a decrease in pro-inflammatory cytokine expression in the olive flounder. These findings emphasize the varied pathogenicity depending on scuticociliate species and suggest that, in addition to the known scuticociliates such as <em>M. avidus</em>, other potential parasites may pose significant risks to olive flounder farms in South Korea.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"166 ","pages":"Article 105357"},"PeriodicalIF":2.7,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143610891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}