FK506 activates the BMP signaling pathway to regulate ovarian development in Portunus trituberculatus

IF 2.7 3区农林科学 Q1 FISHERIES

Developmental and comparative immunology Pub Date : 2025-03-30 DOI:10.1016/j.dci.2025.105365

Xiaocong Chen , Ce Shi , Yangfang Ye , Chunlin Wang , Ronghua Li , Huan Wang , Congcong Hou , Weiwei Song , Changkao Mu

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引用次数: 0

Abstract

Bone morphogenic proteins (BMPs) play important regulatory roles in the development of follicles in mammals. However, studies on the roles of BMPs in ovarian development in low-level aquatic animals, especially the swimming crab Portunus trituberculatus, are limited. In this study, a BMP Ⅰ-type receptor-specific activator (tacrolimus, FK506) was administered at different concentrations via in vivo injection, and the effects of FK506 on the regulation of the BMP signaling pathway during ovarian development in P. trituberculatus were examined. The tissue and cell morphology was observed, and a combined transcriptomics, proteomics and metabolomics analysis was carried out. Crabs administered FK506 exhibited elevated GSI alongside reduced HSI compared to control and blank groups. The main biological processes enriched by joint analysis included lipid metabolism, sugar metabolism, and amino acid metabolism. Fatty acid composition analysis revealed that the activator may activate the BMP signaling pathway to promote ovarian development and accelerate the transport of unsaturated fatty acids from the hepatopancreas to the ovaries. Amino acid metabolism and carbohydrate metabolism provide transporter proteins and energy for lipid metabolism. This study is highly important because it reveals the molecular mechanism by which the BMP signaling pathway regulates gonadal development in a crustacean.

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FK506激活BMP信号通路，调控三瘤兔卵巢发育。

骨形态发生蛋白（BMPs）在哺乳动物卵泡发育中起着重要的调节作用。然而，关于bmp在低水平水生动物，特别是梭子蟹（Portunus trituberculatus）卵巢发育中的作用的研究有限。本研究通过体内注射不同浓度的BMP -Ⅰ型受体特异性激活剂（他克莫司，FK506），研究FK506对三瘤假体卵巢发育过程中BMP信号通路的调控作用。观察组织和细胞形态，并进行转录组学、蛋白质组学和代谢组学联合分析。与对照组和空白组相比，给予FK506的螃蟹表现出GSI升高和HSI降低。通过联合分析富集的主要生物过程包括脂质代谢、糖代谢和氨基酸代谢。脂肪酸组成分析显示，激活物可能激活BMP信号通路，促进卵巢发育，加速不饱和脂肪酸从肝脏和胰腺向卵巢的转运。氨基酸代谢和碳水化合物代谢为脂质代谢提供转运蛋白和能量。该研究揭示了BMP信号通路调控甲壳类动物性腺发育的分子机制，具有重要意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Developmental and comparative immunology 医学-动物学

CiteScore

6.20

自引率

6.90%

发文量

206

审稿时长

49 days

期刊介绍： Developmental and Comparative Immunology (DCI) is an international journal that publishes articles describing original research in all areas of immunology, including comparative aspects of immunity and the evolution and development of the immune system. Manuscripts describing studies of immune systems in both vertebrates and invertebrates are welcome. All levels of immunological investigations are appropriate: organismal, cellular, biochemical and molecular genetics, extending to such fields as aging of the immune system, interaction between the immune and neuroendocrine system and intestinal immunity.