Developmental and comparative immunology最新文献

Identification of a Mnlrig-1 involved in testis reproductive immunity in the oriental river prawn Macrobrachium nipponense 东方对虾Mnlrig-1参与睾丸生殖免疫的鉴定

IF 2.7 3区农林科学

Developmental and comparative immunology Pub Date : 2024-09-24 DOI: 10.1016/j.dci.2024.105273

{"title":"Identification of a Mnlrig-1 involved in testis reproductive immunity in the oriental river prawn Macrobrachium nipponense","authors":"","doi":"10.1016/j.dci.2024.105273","DOIUrl":"10.1016/j.dci.2024.105273","url":null,"abstract":"<div><div>The testis evolves a highly organized testicular microenvironment to support spermatogenesis. However, the knowledge about it is limited in crustacean. In this study, we identified a member of immunoglobulin superfamily (IgSF) from <em>Macrobrachium nipponense</em> testis and explored its roles as a potential pattern recognition receptor (PRR) involved in reproductive immunity. Based on the domains it contains and homology analysis result, we designate it as leucine-rich repeats and immunoglobulin-like domains protein-1 (MnLrig-1). The <em>Mnlrig-1</em> comprises a 3288 bp open reading frame (ORF) encoding a 1095 amino acid protein. MnLrig-1 is consisted of one signaling peptide; one LRR_NT domain; eight LRR domains; five LRR_TYP domains; one LRR_CT domain; three IGc2 regions; one transmembrane region, and C-terminal cytoplasmic tail, sharing similar domains with orthologs in other crustacean species. MnLrig-1 is widely expressed in various tissues of <em>M. nipponense</em>. <em>Mnlrig-1</em> is significantly induced by LPS, PGN, <em>Aeromonas hydrophila</em>, and <em>Vibrio alginolyticus</em> challenge in the testis at 3 h and maintained a high level from 3 h to 24 h. Additionally, two recombinant immunoglobulin domains of MnLrig-1 are obtained, while only one domain shows direct binding affinity towards LPS, PGN, <em>Escherichia coli</em>, <em>A. hydrophila</em>, <em>Staphylococcus aureus</em>, and <em>Bacillus subtilis</em> in vitro. Moreover, silencing <em>Mnlrig-1</em> results in a significant upregulation of three anti-lipopolysaccharide factors (ALFs) in the testis. These results reveal the potential role of MnLrig-1 as a PRR involved in the testis reproductive immunity in <em>M. nipponense</em>. The insights gained from this study will expand our understanding of immune system in crustacean and may have implications for aquaculture and disease management in crustaceans.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142324150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phagocyte dynamics in the blastogenetic cycle of the colonial ascidian Botryllus schlosseri: Cell senescence, segregation and clearance after efferocytosis 无颌腹足类(Botryllus schlosseri)胚泡发生周期中的吞噬细胞动态：细胞衰老、分离和流出后的清除

IF 2.7 3区农林科学

Developmental and comparative immunology Pub Date : 2024-09-19 DOI: 10.1016/j.dci.2024.105271

引用次数: 0

Integrated microRNA study and pathological analysis provides new insights into the immune response of Ruditapes philippinarum under Vibrio anguillarum challenge 微小RNA综合研究和病理分析为菲利宾鱼在鳗弧菌挑战下的免疫反应提供了新见解

IF 2.7 3区农林科学

Developmental and comparative immunology Pub Date : 2024-09-19 DOI: 10.1016/j.dci.2024.105270

{"title":"Integrated microRNA study and pathological analysis provides new insights into the immune response of Ruditapes philippinarum under Vibrio anguillarum challenge","authors":"","doi":"10.1016/j.dci.2024.105270","DOIUrl":"10.1016/j.dci.2024.105270","url":null,"abstract":"<div><div>Manila clam (<em>Ruditapes philippenarum</em>) is an important shellfish aquaculture product. The large-scale breeding of clams is often affected by <em>V. anguillarum</em> and causes large-scale death. However, the pathogenesis, immune response and metabolic pathway of <em>V. anguillarum</em> are still unclear. In this study, we found that the bacterial load in the hepatopancreas of <em>R</em>. <em>philippinarum</em> peaked at 48 h after <em>V. anguillarum</em> infection, and then gradually decreased, while the activity of lysozyme reached the peak at 12 h. Tissue section observation reveals that the infected hepatopancreas cells lost normal structure or necrosis. Additionally, six small RNA libraries were constructed using hepatopancreas of clams. A total of 15 differentially expressed (DE) microRNA (miRNA) were identified at 48 h after <em>V. anguillarum</em> infection, including 8 upregulated and 7 downregulated miRNAs. GO and KEGG enrichment results indicated the prediction of 48 known miRNAs and 127 new miRNAs, with functional annotation suggests that endocytosis pathway and bacterial recognition proteins may play key roles in immune response. The sequencing results were basically consistent with the qRT-PCR validation, indicating the accuracy of the data. This study provides a new idea to explore the immune regulation mechanism of shellfish after <em>V. anguillarum</em> infection, which brings important reference significance for modern immunological research.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142268239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of NK-lysin A, a potent antimicrobial peptide from the zebrafish Danio rerio 斑马鱼NK-lysin A的特征，这是一种来自斑马鱼Danio rerio的强效抗菌肽

IF 2.7 3区农林科学

Developmental and comparative immunology Pub Date : 2024-09-18 DOI: 10.1016/j.dci.2024.105266

{"title":"Characterization of NK-lysin A, a potent antimicrobial peptide from the zebrafish Danio rerio","authors":"","doi":"10.1016/j.dci.2024.105266","DOIUrl":"10.1016/j.dci.2024.105266","url":null,"abstract":"<div><div>Antimicrobial peptides (AMPs) are important players of the innate immune system with a major role in the defense against invading pathogens. AMPs belonging to the family of saposin-like proteins (SAPLIPs) include the porcine NK-lysin and the human granulysin. In the zebrafish <em>Danio rerio</em>, transcript analyses of NK-lysin encoding genes have been reported, but biochemical characterizations at the protein level are missing so far. Here, we present the recombinant expression, purification, and characterization of one of these homologs, namely of NK-lysin A (DaNKlA). To remove the affinity tag from DaNKlA, we made use of a self-splicing intein. Recombinant DaNKlA depolarized liposomes over a broad pH range and showed a preference for negatively charged lipids. DaNKlA inhibited the growth of and killed different Gram-positive and Gram-negative bacteria, including the fish pathogenic bacterium <em>Vibrio anguillarum</em>, by membrane permeabilization but displayed substantially lower activity against yeast cells. Structural modelling and bioinformatic comparison of DaNKlA with characterized SAPLIPs suggest membrane destabilization accompanied by strong electrostatic interactions as the mode of action.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0145305X24001381/pdfft?md5=795ad678195bbad403111190dad15cc0&pid=1-s2.0-S0145305X24001381-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142268238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Studies on immunological characteristics and transcriptomic analysis of Litopenaeus vannamei low salt-tolerance family 万年青低耐盐家族的免疫学特征和转录组分析研究

IF 2.7 3区农林科学

Developmental and comparative immunology Pub Date : 2024-09-13 DOI: 10.1016/j.dci.2024.105265

{"title":"Studies on immunological characteristics and transcriptomic analysis of Litopenaeus vannamei low salt-tolerance family","authors":"","doi":"10.1016/j.dci.2024.105265","DOIUrl":"10.1016/j.dci.2024.105265","url":null,"abstract":"<div><p><em>Litopenaeus vannamei</em> is a widely distributed euryhaline aquatic animal, affected by low salinity, which can impact its disease resistance and immunity. However, there is a limited understanding of the adaptation mechanisms of <em>L</em>. <em>vannamei</em> with different genetic backgrounds to low salinity. Therefore, the present study aimed to compare the immunity characteristics and transcriptomics of <em>L</em>. <em>vannamei</em> low salt-tolerant (FG I/J) and low salt-sensitive (control) families. Also, the disease resistance and immune parameters (including [THC], hemolymph cell viability, lysozyme activity [LZM], phenoloxidase content [PO], interleukin-6 [IL-6], and tumor necrosis factor-alpha [TNF-α]) of the FG I/J and control families of <em>L</em>. <em>vannamei</em> under low salinity (5‰) and ambient salinity (24‰) were examined. Additionally, hepatopancreas transcriptomics of the FG I/J and control families were analyzed at a salinity of 5‰. The results showed that the FG I/J family had higher disease resistance to <em>Vibrio parahaemolyticus</em> and stronger immunological capacity than the control family. Transcriptomic analysis showed significantly enriched energy metabolism and immune regulation pathways. Therefore, we speculated that energy metabolism provides sufficient energy for immunological modulation in the FG I/J family to deal with long-term low-salt stress and achieve high growth and survival rates.</p></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142196889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oral administration enhances directly mucosal immune system in intestine of olive flounder (Paralichthys olivaceus) 口服药物可直接增强橄榄鲽肠道粘膜免疫系统的功能

IF 2.7 3区农林科学

Developmental and comparative immunology Pub Date : 2024-09-11 DOI: 10.1016/j.dci.2024.105262

{"title":"Oral administration enhances directly mucosal immune system in intestine of olive flounder (Paralichthys olivaceus)","authors":"","doi":"10.1016/j.dci.2024.105262","DOIUrl":"10.1016/j.dci.2024.105262","url":null,"abstract":"<div><p>Aquaculture is notably vulnerable to diseases, with <em>Edwardsiella tarda</em> causing significant mortality across various commercially important fish species in both freshwater and marine environments. In the aquaculture industry, sustainable disease control hinges on the effective development of vaccines. Oral vaccines present an appealing approach to immunization in fish due to their ease of antigen administration, reduced stress compared to non-oral delivery methods, and their potential applicability to both small and large finfish species. In mammals, the exposure of mucosal surfaces to antigens results in the secretion of antigen-specific IgA at these locations. Mammals have a common mucosal immune system, in which stimulation of one epithelium can also give rise to specific IgA or IgM responses in other mucosal organs. Mucosal immunoglobulins are particularly important in developing vaccines that provide mucosal immunity. However, it remains unclear whether fish share a common mucosal system. Moreover, neither Peyer's patches nor intestinal lymph nodes were identified. Nevertheless, oral vaccination remains an attractive method for inducing immunity. We investigated whether the activation of the mucosal immune response was induced by direct injection of the antigen. After oral antigen administration, antigen-specific antibody titers increased in the experimental group (<em>E. tarda</em> FKC vaccine). In the challenge experiment, the cumulative survival rate was 72% (<em>E. tarda</em>). This suggests that oral administration of antigens can activate intestinal mucosal immunity in flounders. Additionally, these results help understand the intestinal mucosal immune system of teleost fish. In the future, research on the signaling mechanisms of these genes is expected to provide helpful information for developing vaccine adjuvants.</p></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142196890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of NLRP3 inflammasome components in the endangered Chinese giant salamander (Andrias davidianus) 濒危中国大鲵体内 NLRP3 炎症小体成分的特征描述

IF 2.7 3区农林科学

Developmental and comparative immunology Pub Date : 2024-09-10 DOI: 10.1016/j.dci.2024.105263

{"title":"Characterization of NLRP3 inflammasome components in the endangered Chinese giant salamander (Andrias davidianus)","authors":"","doi":"10.1016/j.dci.2024.105263","DOIUrl":"10.1016/j.dci.2024.105263","url":null,"abstract":"<div><p>Chinese giant salamander (<em>Andrias davidianus</em>) is the largest extant urodela species and has unique evolutionary position. Studying the immune system of Chinese giant salamander contributes to understanding the evolution of immune systems of vertebrates. The NLR-related protein 3 (NLRP3) inflammasome comprised of NLRP3, ASC and caspase-1 play important roles in the host innate immunity. However, little is know about the NLRP3 inflammasome components in Chinese giant salamander. In this study, the NLRP3, apoptosis-associated speck-like protein (ASC) and caspase-1 (adaNLRP3, adaASC and adaCaspase-1) were characterized from Chinese giant salamander. The proteins of these three genes shared similar motifs and structures with their mammalian counterparts, with a PYD motif, a nucleotide-binding domain (NACHT) motif, and four leucine-rich repeat domain (LRR) motifs identified in adaNLRP3, a pyrin domain (PYD) motif and a caspase recruitment domain (CARD) motif in adaASC, and a CARD motif and a CASc motif in adaCaspase-1. These three genes were constitutively expressed in the skin, heart, lung, kidney, muscle, brain, spleen, and liver of Chinese giant salamander. Following <em>Aeromonas hydrophia</em> infection, all the three genes were up-regulated in various tissues. Molecular docking analysis revealed that the key residues involved in forming the adaNLRP3/adaASC complex were located in the PYD motifs, and that involved in forming the adaASC/adaCaspase-1 complex were located in the CARD motifs. Further analysis revealed that the hydrogen bonds and salt bridges had crucial roles in the formation of adaNLRP3/acaASC and adaASC/adaCaspase-1 complexes. To the best of our knowledge, this is the first report on the NLRP3 inflammasome components in Chinese giant salamander which will be helpful in further understanding the function of the NLRP3 inflammasome and in elucidating its role in the immune response to microbes.</p></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142196888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First report of a chemokine from camelids: Dromedary CXCL8 is induced by poxvirus and heavy metal toxicity 首次报道驼科动物的趋化因子：单峰驼的 CXCL8 由痘病毒和重金属毒性诱导。

IF 2.7 3区农林科学

Developmental and comparative immunology Pub Date : 2024-09-05 DOI: 10.1016/j.dci.2024.105261

{"title":"First report of a chemokine from camelids: Dromedary CXCL8 is induced by poxvirus and heavy metal toxicity","authors":"","doi":"10.1016/j.dci.2024.105261","DOIUrl":"10.1016/j.dci.2024.105261","url":null,"abstract":"<div><p>Low molecular weight proteins, known as chemokines, facilitate the migration and localization of immune cells to the site of infection and injury. One of the first chemokines identified, CXCL8 functions as a key neutrophil activator, recruiting neutrophils to sites of inflammation. Several viral infections, including zoonotic coronaviruses and poxviruses, have been reported to induce the expression of CXCL8. Dromedary camels are known to harbor several potentially zoonotic pathogens, but critical immune molecules such as chemokines remain unidentified. We report here the identification of CXCL8 from the dromedary camel - the first chemokine identified from camelids. The complete dromedary CXCL8 cDNA sequence as well as the corresponding gene sequence from dromedary and two New World camelids - alpaca and llama were cloned. CXCL8 mRNA expression was relatively higher in PBMC, spleen, lung, intestine, and liver. Poly(I:C) and lipopolysaccharide stimulated CXCL8 expression <em>in vitro</em>, while interferon treatment inhibited it. <em>In vitro</em> infection with potentially zoonotic camelpox virus induced the expression of CXCL8 in camel kidney cells. Toxicological studies on camelids have been limited, and no biomarkers have been identified. Hence, we also evaluated CXCL8 mRNA expression as a potential biomarker to assess heavy metal toxicity in camel kidney cells <em>in vitro</em>. CXCL8 expression was increased after <em>in vitro</em> exposure to heavy metal compounds of cobalt and cadmium, suggesting potential utility as a biomarker for renal toxicity in camels. The results of our study demonstrate that camel CXCL8 plays a significant role in immunomodulatory and induced toxicity responses in dromedary camels.</p></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142145374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancement of immunity and disease resistance in Litopenaeus vannamei through injection of tyramine formulated with polyethylene glycol 通过注射用聚乙二醇配制的酪胺增强万年青的免疫力和抗病性。

IF 2.7 3区农林科学

Developmental and comparative immunology Pub Date : 2024-09-03 DOI: 10.1016/j.dci.2024.105260

{"title":"Enhancement of immunity and disease resistance in Litopenaeus vannamei through injection of tyramine formulated with polyethylene glycol","authors":"","doi":"10.1016/j.dci.2024.105260","DOIUrl":"10.1016/j.dci.2024.105260","url":null,"abstract":"<div><p>This study investigates the prolonged effect of immune disease resistance in <em>Litopenaeus vannamei</em> through the administration of tyramine (TA) formulated with polyethylene glycol (PEG). Facing the challenges of intensive farming, environmental stress, and global climate changes, innovative approaches to improve shrimp health are essential. The research focuses on the role of biogenic amines in stress response and immune regulation, demonstrating that TA, especially when combined with PEG, significantly prolongs immunity and resistance against <em>Vibrio alginolyticus</em>. The experimental design included administering TA, PEG, and TA-PEG, followed by evaluations of immunity, lactate and glucose levels, and immune-related gene expressions. Results showed notable prolonged effects in total hemocyte count, phenoloxidase activity, and phagocytic activity in the TA-PEG group, indicating enhanced immune activation period. Additionally, the expression of prophenoloxidase system-related genes was significantly upregulated in the TA-PEG group. Furthermore, the TA-PEG group exhibited a significantly higher survival rate in a susceptibility test against <em>V. alginolyticus</em>. The results of this study confirm that the combined use of PEG can effectively extend the immunostimulatory duration of TA.</p></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142139592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The bright future of developmental and comparative immunology: Views from heroes of the field 发育和比较免疫学的光明前景：该领域英雄们的观点

IF 2.7 3区农林科学

Developmental and comparative immunology Pub Date : 2024-08-30 DOI: 10.1016/j.dci.2024.105257

引用次数: 0