Developmental and comparative immunology最新文献

Cloning and RNA interference indicate that the Na/K-ATPase-β gene helps Hong Kong oysters (Crassostrea hongkongensis) resist short-term hypersalinity stress. 克隆和RNA干扰表明，Na/ k - atp酶-β基因有助于香港牡蛎（Crassostrea hongkongensis）抵抗短期高盐胁迫。

IF 2.7 3区农林科学

Developmental and comparative immunology Pub Date : 2025-05-27 DOI: 10.1016/j.dci.2025.105397

Lingxin Kong, Ziao Chen, Yeshao Peng, Qiong Deng, Zhen Jia, Peng Zhu, Youhou Xu, Zhicai She

{"title":"Cloning and RNA interference indicate that the Na/K-ATPase-β gene helps Hong Kong oysters (Crassostrea hongkongensis) resist short-term hypersalinity stress.","authors":"Lingxin Kong, Ziao Chen, Yeshao Peng, Qiong Deng, Zhen Jia, Peng Zhu, Youhou Xu, Zhicai She","doi":"10.1016/j.dci.2025.105397","DOIUrl":"https://doi.org/10.1016/j.dci.2025.105397","url":null,"abstract":"<p><p>Na/K-ATPase is a carrier protein embedded in the cell membrane. The main function of this protein is to maintain osmotic pressure balance inside and outside the cell. To investigate the role of the Na/K-ATPase gene under high-salt stress in the Hong Kong oyster (Crassostrea hongkongensis), full-length cloning and expression analysis of Na/K-ATPase β subunit (ChNa/K-ATPase-β) were performed. Additionally, the gene function was verified using RNA interference (RNAi) technology. In this study, we obtained a 1230 bp sequence of the ChNa/K-ATPase-β gene, including a 5' untranslated region (UTR) of 25 bp; a 3' UTR of 263 bp; and an open reading frame of 942 bp, encoding a protein of 313 amino acids, with a molecular weight 35.452 kDa and a theoretical isoelectric point 5.55. Reverse transcription-quantitative polymerase chain reaction revealed the expression of ChNa/K-ATPase-β in all tissues of Hong Kong oyster, with the highest relative expression level in the blood sinus, followed by the gills, and the lowest level of expression in the labial palps. Under hypersalinity stress (30 PSU), the expression levels of ChNa/K-ATPase-β in the gills significantly upregulated at 8 h, downregulated at 24 h, and showed no significant change between 48-120 h. RNAi-mediated knockdown of ChNa/K-ATPase-β led to a concomitant downregulation of ChNa/K-ATPase-α expression. After interfering with ChNa/K-ATPase-β for 48, 72, 96, and 120 h, the Na/K-ATPase activity in the gills significantly decreased. The survival rate of the RNAi group (75%) was lower than that of the blank control and negative control groups, which proved that Na/K-ATPase dysfunction would seriously affect the resistance of oysters to high salt stress. These results indicate that ChNa/K-ATPase-β plays an important role in enhancing the salt stress resistance in the Hong Kong oyster.</p>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":" ","pages":"105397"},"PeriodicalIF":2.7,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144181651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cellular immune senescence in mosquitoes accelerates when the temperature is warmer 温度升高时，蚊子的细胞免疫衰老会加速

IF 2.7 3区农林科学

Developmental and comparative immunology Pub Date : 2025-05-22 DOI: 10.1016/j.dci.2025.105396

Jordyn S. Barr, Saksham R. Saksena, Julián F. Hillyer

{"title":"Cellular immune senescence in mosquitoes accelerates when the temperature is warmer","authors":"Jordyn S. Barr, Saksham R. Saksena, Julián F. Hillyer","doi":"10.1016/j.dci.2025.105396","DOIUrl":"10.1016/j.dci.2025.105396","url":null,"abstract":"<div><div>Mosquitoes are poikilotherms and ectotherms, so their body temperature is predicated by the temperature of their environment. As the temperature rises, development and metabolism quicken. At the same time, immune strength weakens with aging, a process called senescence. Aging can be characterized as a function of time (chronological age) or as a function of how well the body operates (physiological age), and we predict that warmer temperature decouples chronological and physiological aging, accelerating immune senescence. We evaluated how warmer temperature and aging interact to alter cellular immunity in the mosquito, <em>Anopheles gambiae</em>, by rearing them at three temperatures and quantifying the number of immune cells, called hemocytes, and their phagocytic activity at four ages. We discovered that the number of circulating hemocytes decreases with warmer temperature and aging, and that the aging-dependent decrease occurs faster when the temperature is warmer. However, the number of sessile hemocytes attached to the dorsal abdominal wall increases with infection and warmer temperature but decreases with aging. When a mosquito is infected, the aging-dependent decrease in the number of sessile hemocytes occurs faster when the temperature is warmer. Although the number of hemocytes decreases with aging, the phagocytic activity of individual hemocytes increases, with the aging-dependent increase occurring earlier when the temperature is warmer. Overall, warmer temperature accelerates senescence of the cellular immune response in mosquitoes, which has implications for how poikilotherms and ectotherms fight infections as they age in our warming world.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"168 ","pages":"Article 105396"},"PeriodicalIF":2.7,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144125037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The classical MHC class I and II genes of O. m. formosanus exhibit different polymorphism levels 台湾人经典MHCⅰ类和ⅱ类基因表现出不同的多态性水平。

IF 2.7 3区农林科学

Developmental and comparative immunology Pub Date : 2025-05-22 DOI: 10.1016/j.dci.2025.105392

Zhi-Wei Zhang , Lukas A. Fugmann , Shu Yuan Yang , Jin-Chywan Gwo , Sebastian D. Fugmann

{"title":"The classical MHC class I and II genes of O. m. formosanus exhibit different polymorphism levels","authors":"Zhi-Wei Zhang , Lukas A. Fugmann , Shu Yuan Yang , Jin-Chywan Gwo , Sebastian D. Fugmann","doi":"10.1016/j.dci.2025.105392","DOIUrl":"10.1016/j.dci.2025.105392","url":null,"abstract":"<div><div><em>Oncorhynchus masou formosanus</em> (Formosan landlocked salmon) is a salmonid fish endemic to Taiwan with a critically endangered extant small population in high-altitude mountain streams. To begin to assess the remaining genetic diversity we characterized the single classical MHC class I (<em>UBA</em>) and MHC class II (<em>DAA</em> and <em>DAB</em>) genes of teleost fish in a small cohort of eight randomly collected <em>O. m. formosanus</em> individuals whose relationship is unknown. We focused on the exons encoding the peptide binding regions of these complexes as they are considered the most highly polymorphic regions in vertebrate genomes. Surprisingly, the <em>DAA</em> and <em>DAB</em> genes appeared homozygous and identical among all eight fish indicating that the encoded class II complex is monomorphic. In contrast, three distinct <em>UBA</em> alleles were discovered with one dominant allele present in every single individual. Notably, 75 % were heterozygous indicating a selective advantage of heterozygosity at this locus. Lastly, our MHC alleles differ from those present in the genome of the closely related Japanese <em>O. m. masou</em>, suggesting that the loss of DAA/DAB diversity and the emergence of the dominant UBA allele occurred after their populations were isolated. Together we discovered residual genetic diversity at the classical MHC class I locus in <em>O. m. formosanus</em> and maintaining this allelic variation unlike the homozygous <em>DAA</em> and <em>DAB</em> genes is likely important for its survival in its geographically restricted unique habitat.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"168 ","pages":"Article 105392"},"PeriodicalIF":2.7,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144141710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling salivary gland-specific gene expression of Piezo1 and Neuroendocrine in the leech, Helobdella austinensis 揭示水蛭唾液腺特异性基因Piezo1和神经内分泌的表达

IF 2.7 3区农林科学

Developmental and comparative immunology Pub Date : 2025-05-21 DOI: 10.1016/j.dci.2025.105391

In-Hyeok Pyo , Yoo-Bin Yoon , Geon-Hwi Jeong , Soon Cheol Park , Geon-Woo Lee , Yam Prasad Aryal , Hee-Jin Kwak , Sung-Jin Cho

{"title":"Unveiling salivary gland-specific gene expression of Piezo1 and Neuroendocrine in the leech, Helobdella austinensis","authors":"In-Hyeok Pyo , Yoo-Bin Yoon , Geon-Hwi Jeong , Soon Cheol Park , Geon-Woo Lee , Yam Prasad Aryal , Hee-Jin Kwak , Sung-Jin Cho","doi":"10.1016/j.dci.2025.105391","DOIUrl":"10.1016/j.dci.2025.105391","url":null,"abstract":"<div><div>Mechanotransduction is a critical biological phenomenon in living organisms, with <em>Piezo1</em> being one of the key mechanotransduction ion channel genes. <em>Piezo1</em> is widely expressed across various tissues and organs, playing critical roles in numerous biological processes, including innate and adaptive immune activation. While most studies on <em>Neuroendocrine</em> and <em>Piezo</em> functions have focused on vertebrates and higher invertebrates (e.g., <em>Drosophila</em>), however research in lophotrochozoan animal models remains scarce. To address this gap, we utilized <em>Helobdella austinensis</em> (phylum: Annelida) to investigate the putative function of <em>Piezo1</em> and uncovered evidence related to the neuroendocrine system through spatiotemporal characterization. Our findings represent the developmental contribution of <em>Piezo1</em> from early to late embryonic stages by demonstrating its expression in a lophotrochozoan. Intriguingly, the expression of <em>Hau-Piezo1</em> was specifically detected in salivary gland-related precursors and tissues during development. Additionally, <em>Neuroendocrine</em> expression was observed in a lophotrochozoan, suggesting the correlation between neuronal stimulation and immune cells along the salivary glands of leeches. Furthermore, the downregulation of <em>Hau-Piezo1</em> following bacterial challenge suggests that Piezo1 plays a role in regulating inflammatory responses. Taken together, we characterized the spatiotemporal expression pattern of <em>Hau-Piezo1</em> in leeches and demonstrated its conserved and diversified functions based on its phylogenetic relationship with other homologs. These results suggest that <em>Piezo1</em> may serve as a salivary gland marker in leeches and provide evidence for the presence of immune cells along the salivary glands in lophotrochozoans.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"168 ","pages":"Article 105391"},"PeriodicalIF":2.7,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144125036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biphasic role of L-TGF-β2 in modulating lamprey inflammation: Insights into vertebrate immune evolution L-TGF-β2在调节七鳃鳗炎症中的双相作用：对脊椎动物免疫进化的见解

IF 2.7 3区农林科学

Developmental and comparative immunology Pub Date : 2025-05-01 DOI: 10.1016/j.dci.2025.105372

Hao Wang , Junfu Guo , Xuanyi Chen , Siqi Liu , Wenna Li , Lu Yang , Jianmiao Wang , Yinglun Han

{"title":"Biphasic role of L-TGF-β2 in modulating lamprey inflammation: Insights into vertebrate immune evolution","authors":"Hao Wang , Junfu Guo , Xuanyi Chen , Siqi Liu , Wenna Li , Lu Yang , Jianmiao Wang , Yinglun Han","doi":"10.1016/j.dci.2025.105372","DOIUrl":"10.1016/j.dci.2025.105372","url":null,"abstract":"<div><div>The regulation of inflammatory balance is central to immune homeostasis, with disruptions contributing to autoimmune diseases. As representatives of early vertebrates, lampreys offer a valuable model for investigating the evolutionary foundations of immune regulation. This study examines the dual role of L-TGF-β2 in modulating inflammation in lampreys, providing insights into the evolutionary origins of immune homeostasis mechanisms. Using lipopolysaccharide (LPS)-induced inflammation models, recombinant L-TGF-β2 was found to enhance leukocyte chemotaxis in quiescent states while inhibiting excessive migration during activation. Quantitative PCR revealed that L-TGF-β2 stimulates pro-inflammatory cytokine expression during early inflammatory phases and attenuates it during resolution. Tissue-specific expression patterns of TGF-β receptors indicated their potential role in mediating the distinct regulatory effects of L-TGF-β2 across diverse immune environments. These findings align with the biphasic functions of TGF-β observed in higher vertebrates, underscoring the evolutionary conservation of this signaling pathway. This research enhances understanding the functional versatility of TGF-β signaling and its pivotal role in vertebrate immune evolution, providing insights into ancient mechanisms of immune homeostasis and their modern implications for inflammatory disease research.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"167 ","pages":"Article 105372"},"PeriodicalIF":2.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143898627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrative analysis of the microRNA and mRNA response of barramundi (Lates calcarifer) under acute cold stress and Vibrio harveyi challenge barramundi （Lates calcarifer）在急性冷应激和哈维弧菌胁迫下的microRNA和mRNA响应综合分析

IF 2.7 3区农林科学

Developmental and comparative immunology Pub Date : 2025-05-01 DOI: 10.1016/j.dci.2025.105385

Roisin Sullivan , Joy A. Becker , Francisca Samsing

{"title":"Integrative analysis of the microRNA and mRNA response of barramundi (Lates calcarifer) under acute cold stress and Vibrio harveyi challenge","authors":"Roisin Sullivan , Joy A. Becker , Francisca Samsing","doi":"10.1016/j.dci.2025.105385","DOIUrl":"10.1016/j.dci.2025.105385","url":null,"abstract":"<div><div>Barramundi (<em>Lates calcarifer</em>) are emerging as a key species in warm-water aquaculture worldwide; however, disease outbreaks caused by <em>Vibrio</em> spp. are impeding industry expansion. Climate change is expected to exacerbate this issue by intensifying extreme weather events, including unusually cold temperatures, thereby increasing the risk of disease. In this study, we investigated the combined effect of cold stress and <em>V. harveyi</em> infection on the early transcriptome (mRNA) and microRNA responses of juvenile barramundi to enhance our understanding of host-pathogen interactions. High levels of differential gene expression were observed in fish subjected to cold stress (22 °C) post-infection with <em>V. harveyi</em>, with 3231 differentially expressed genes and an extensive pro-inflammatory immune response. In contrast, most differentially expressed microRNAs were associated with fish infected with <em>V. harveyi</em> housed under optimal temperature conditions (30 °C). MicroRNAs play a crucial role in regulating gene expression, typically through downregulation of target mRNAs. The significant upregulation of miRNAs in barramundi kept at 30 °C, and the lack of miRNA upregulation in cold stressed fish, suggests that cold stress impaired the immune-regulatory capacity of affected fish, resulting in a hyper-inflammatory response that may account for the increased mortality observed. This study is the first dual study of the transcriptome and microRNA response of barramundi to <em>V. harveyi</em> infection and expands understanding of the innate immune response in barramundi and the regulatory role of microRNAs in teleost fish.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"167 ","pages":"Article 105385"},"PeriodicalIF":2.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143935514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel complement C1q A chain from marbled flounder, Pseudopleuronectes yokohamae: genome characterization, expression and potential role in antibacterial immunity 一种新的补体C1q A链来自大理石比目鱼，Pseudopleuronectes yokohamae：基因组表征，表达及其在抗菌免疫中的潜在作用

IF 2.7 3区农林科学

Developmental and comparative immunology Pub Date : 2025-05-01 DOI: 10.1016/j.dci.2025.105389

Shuai Wang , Hui Liu , Zheng Zhang , Tingting He , XuYa Wei , Yaming Zhang , Yan Chen , Xuejie Li , Wei Wang

{"title":"A novel complement C1q A chain from marbled flounder, Pseudopleuronectes yokohamae: genome characterization, expression and potential role in antibacterial immunity","authors":"Shuai Wang , Hui Liu , Zheng Zhang , Tingting He , XuYa Wei , Yaming Zhang , Yan Chen , Xuejie Li , Wei Wang","doi":"10.1016/j.dci.2025.105389","DOIUrl":"10.1016/j.dci.2025.105389","url":null,"abstract":"<div><div>This study systematically analyzed the molecular characteristics, tissue expression, and function of the marbled flounder (<em>Pseudopleuronectes yokohamae</em>) C1qA protein (<em>Py</em>C1qA) in fish immunity. <em>Py</em>C1qA contains a signal peptide, collagen-like region, and globular head. Its amino acid sequence and globular structure are highly conserved among multiple species, especially at key sites for pathogen recognition. Phylogenetic analysis showed that <em>Py</em>C1qA has a close evolutionary relationship with other marine fish (such as marbled flounder and European flounder) but is different from freshwater fish. Tissue expression analysis showed that <em>Py</em>C1qA is highly expressed in the skin, and pathogen stimulation experiments showed that its expression changes dynamically in multiple immune-related tissues, indicating that it plays an important role in humoral immunity. Functional studies have shown that recombinant <em>Py</em>C1qA (r<em>Py</em>C1qA) can bind to a variety of fish pathogens and significantly enhance the hemolytic and bactericidal abilities of fish serum. Co-immunoprecipitation experiments verified the specific interaction between <em>Py</em>C1qA and IgM, further supporting its role in regulating humoral immunity through the classical complement pathway. In addition, in vivo experiments showed that r<em>Py</em>C1qA inhibited the proliferation of pathogens in immune-related tissues, demonstrating its potential anti-infection ability. This study revealed for the first time the molecular and functional characteristics of marbled flounder C1qA protein, expanded our understanding of the fish complement system in immune defense, and provided an important theoretical basis for the prevention and control of aquaculture diseases.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"167 ","pages":"Article 105389"},"PeriodicalIF":2.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143935515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long-term and non-specific immune memory induced by muramyl dipeptide and its protective effect against Vibrio anguillarum in rainbow trout (Oncorhynchus mykiss) 虹鳟muramyl二肽诱导的长期非特异性免疫记忆及其对鳗弧菌的保护作用

IF 2.7 3区农林科学

Developmental and comparative immunology Pub Date : 2025-05-01 DOI: 10.1016/j.dci.2025.105386

Kyung Min Lee, Ki Hong Kim

{"title":"Long-term and non-specific immune memory induced by muramyl dipeptide and its protective effect against Vibrio anguillarum in rainbow trout (Oncorhynchus mykiss)","authors":"Kyung Min Lee, Ki Hong Kim","doi":"10.1016/j.dci.2025.105386","DOIUrl":"10.1016/j.dci.2025.105386","url":null,"abstract":"<div><div>Muramyl dipeptide (MDP), a minimal bioactive peptidoglycan motif derived from the bacterial cell wall, is known to trigger a wide range of signaling cascades related to inflammatory and antibacterial responses, thereby enhancing the antimicrobial function of various innate immune cells including monocytes, macrophages, neutrophils, natural killer (NK) cells, and lymphocytes. To date, several studies have demonstrated that MDP has the capacity to stimulate non-specific immunity in fish. However, the long-term effect of MDP on fish remains unclear. In this study, we aimed to investigate the long-term protective effect of MDP against <em>Vibrio anguillarum</em> in rainbow trout (<em>Oncorhynchus mykiss</em>). MDP was administered to the rainbow trout by intraperitoneal (IP) injection, and the bacterial challenge was conducted at 4 weeks post-administration. The MDP-injected fish exhibited significantly higher survival rates than fish injected with PBS. Following bacterial infection, significantly reduced bacterial loads were shown in the head kidney of MDP-injected fish, accompanied by elevated expression levels of TNF-α, IL-1β, IL-6, IL-12, and HIF-1α. Furthermore, a significant increase of acetylation of histone 3 at lysine 27 (H3K27ac) was evident at the promoter regions of TNF-α and IL-1β in the fish of the MDP group at 4 weeks post-administration. These results suggest that MDP-induced histone acetylation in immune-related genes’ promoters enhanced immune gene expression upon infection, possibly contributing to the observed long-term antibacterial effect and protection against <em>V. anguillarum</em>.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"167 ","pages":"Article 105386"},"PeriodicalIF":2.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143916205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Myd88 functions as a negative regulator of IRF11-mediated IFN production in teleosts Myd88在硬鱼中作为irf11介导的IFN产生的负调节因子

IF 2.7 3区农林科学

Developmental and comparative immunology Pub Date : 2025-05-01 DOI: 10.1016/j.dci.2025.105388

Chao Xiang , Wenxing Li , Xiaoling Zhou , DongLi Li , Wenshu Huang , Pin Nie , Bei Huang

{"title":"Myd88 functions as a negative regulator of IRF11-mediated IFN production in teleosts","authors":"Chao Xiang , Wenxing Li , Xiaoling Zhou , DongLi Li , Wenshu Huang , Pin Nie , Bei Huang","doi":"10.1016/j.dci.2025.105388","DOIUrl":"10.1016/j.dci.2025.105388","url":null,"abstract":"<div><div>MyD88 is an intracellular adaptor protein that mediates signals from many TLRs and IL-1/IL-18 receptors to downstream kinases, by recruiting transcription factors, such as IFN regulatory factor (IRF) family members, to evoke the activation of a series of target genes. Recent studies imply a role of MyD88 in the regulation of type IFN signaling through MyD88-IRF interaction in teleosts. In this study, we explore the mechanistic role of MyD88 on IFN production mediated by IRF11, a fish-specific positive regulator in this pathway. Overexpression of MyD88 suppressed IRF11-induced activation of the IFN promoter. Conversely, MyD88 knockdown in zebrafish liver cells further increased IRF11-induced expression of IFN and IFN-stimulated genes (ISGs). Co-immunoprecipitation experiments identified the Toll/IL-1 receptor (TIR) domain of MyD88 and the region between amino acids 114 and 155 of IRF11 as critical for their interaction. Confocal microscopy and subcellular fractionation revealed that MyD88 co-localizes with IRF11 in the nucleus upon co-expression. These results suggest MyD88 negatively regulates IRF11-mediated IFN production through protein-protein interaction and nuclear co-localization. Thus our present study places MyD88 as negative regulator participating in IRF11-mediated IFN production and provides a novel mechanism for regulating the fish antiviral response.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"167 ","pages":"Article 105388"},"PeriodicalIF":2.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143949065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and functional analysis of glutathione S-transferase gene family in Trachinotus ovatus: Transcriptomic response to environmental and pathogenic stress 卵形沙眼谷胱甘肽s转移酶基因家族的鉴定和功能分析：对环境和致病胁迫的转录组反应

IF 2.7 3区农林科学

Developmental and comparative immunology Pub Date : 2025-05-01 DOI: 10.1016/j.dci.2025.105371

Jia-Mei Zhou , Teng-Fei Zhu , Hua-Yang Guo , Lin Xian , Bao-Suo Liu , Nan Zhang , Tian-Yue Zhang , Ke-Cheng Zhu , Dian-Chang Zhang

{"title":"Identification and functional analysis of glutathione S-transferase gene family in Trachinotus ovatus: Transcriptomic response to environmental and pathogenic stress","authors":"Jia-Mei Zhou , Teng-Fei Zhu , Hua-Yang Guo , Lin Xian , Bao-Suo Liu , Nan Zhang , Tian-Yue Zhang , Ke-Cheng Zhu , Dian-Chang Zhang","doi":"10.1016/j.dci.2025.105371","DOIUrl":"10.1016/j.dci.2025.105371","url":null,"abstract":"<div><div>Glutathione S-transferases (<em>GSTs)</em> are a class of enzymes that perform a wide range of biological functions, particularly playing central roles in detoxification, antioxidant processes, and protecting against environmental and pathogen stresses. In this study, we conducted a comprehensive genomic analysis of the <em>GST g</em>ene family in <em>Trachinotus ovatus</em>, identifying a total of 14 <em>GST</em> genes. Their chromosomal distribution, characterization, evolutionary relationships, evidence of positive selection were studied in detail. It was found that the <em>GST</em> genes are evolutionarily conserved. Phylogenetic analyses revealed that these genes are distributed into three subgroups: cytosolic GSTs, mitochondrial GSTs<em>,</em> and membrane-associated proteins in eicosanoid and glutathione metabolism (MAPEG). We further explored the physiological responses of these genes to hypoxic stress and exposure to bacterial pathogens, particularly noting the significant up-regulation of <em>ToGSTT1</em>, <em>ToGSTT2</em>, <em>ToGSTK1b</em>, and <em>ToGSTT3</em> during reoxygenation of gill tissues, as well as the relevance of <em>ToGSTK1a</em> and <em>ToGSTK1b</em> in immunomodulation in response to both <em>Streptococcus agalactiae</em> and <em>Cryptocaryon irritans</em> infections. This study enhances our understanding of the functional characteristics of the <em>GST</em> gene family in <em>T. ovatus</em> and provides new insights into its roles in antioxidative stress and immune response mechanisms.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"167 ","pages":"Article 105371"},"PeriodicalIF":2.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143892110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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