Developmental and comparative immunology最新文献

{"title":"Biphasic role of L-TGF-β2 in modulating lamprey inflammation: Insights into vertebrate immune evolution","authors":"Hao Wang ,&nbsp;Junfu Guo ,&nbsp;Xuanyi Chen ,&nbsp;Siqi Liu ,&nbsp;Wenna Li ,&nbsp;Lu Yang ,&nbsp;Jianmiao Wang ,&nbsp;Yinglun Han","doi":"10.1016/j.dci.2025.105372","DOIUrl":"10.1016/j.dci.2025.105372","url":null,"abstract":"<div><div>The regulation of inflammatory balance is central to immune homeostasis, with disruptions contributing to autoimmune diseases. As representatives of early vertebrates, lampreys offer a valuable model for investigating the evolutionary foundations of immune regulation. This study examines the dual role of L-TGF-β2 in modulating inflammation in lampreys, providing insights into the evolutionary origins of immune homeostasis mechanisms. Using lipopolysaccharide (LPS)-induced inflammation models, recombinant L-TGF-β2 was found to enhance leukocyte chemotaxis in quiescent states while inhibiting excessive migration during activation. Quantitative PCR revealed that L-TGF-β2 stimulates pro-inflammatory cytokine expression during early inflammatory phases and attenuates it during resolution. Tissue-specific expression patterns of TGF-β receptors indicated their potential role in mediating the distinct regulatory effects of L-TGF-β2 across diverse immune environments. These findings align with the biphasic functions of TGF-β observed in higher vertebrates, underscoring the evolutionary conservation of this signaling pathway. This research enhances understanding the functional versatility of TGF-β signaling and its pivotal role in vertebrate immune evolution, providing insights into ancient mechanisms of immune homeostasis and their modern implications for inflammatory disease research.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"167 ","pages":"Article 105372"},"PeriodicalIF":2.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143898627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and functional analysis of glutathione S-transferase gene family in Trachinotus ovatus: Transcriptomic response to environmental and pathogenic stress","authors":"Jia-Mei Zhou ,&nbsp;Teng-Fei Zhu ,&nbsp;Hua-Yang Guo ,&nbsp;Lin Xian ,&nbsp;Bao-Suo Liu ,&nbsp;Nan Zhang ,&nbsp;Tian-Yue Zhang ,&nbsp;Ke-Cheng Zhu ,&nbsp;Dian-Chang Zhang","doi":"10.1016/j.dci.2025.105371","DOIUrl":"10.1016/j.dci.2025.105371","url":null,"abstract":"<div><div>Glutathione S-transferases (<em>GSTs)</em> are a class of enzymes that perform a wide range of biological functions, particularly playing central roles in detoxification, antioxidant processes, and protecting against environmental and pathogen stresses. In this study, we conducted a comprehensive genomic analysis of the <em>GST g</em>ene family in <em>Trachinotus ovatus</em>, identifying a total of 14 <em>GST</em> genes. Their chromosomal distribution, characterization, evolutionary relationships, evidence of positive selection were studied in detail. It was found that the <em>GST</em> genes are evolutionarily conserved. Phylogenetic analyses revealed that these genes are distributed into three subgroups: cytosolic GSTs, mitochondrial GSTs<em>,</em> and membrane-associated proteins in eicosanoid and glutathione metabolism (MAPEG). We further explored the physiological responses of these genes to hypoxic stress and exposure to bacterial pathogens, particularly noting the significant up-regulation of <em>ToGSTT1</em>, <em>ToGSTT2</em>, <em>ToGSTK1b</em>, and <em>ToGSTT3</em> during reoxygenation of gill tissues, as well as the relevance of <em>ToGSTK1a</em> and <em>ToGSTK1b</em> in immunomodulation in response to both <em>Streptococcus agalactiae</em> and <em>Cryptocaryon irritans</em> infections. This study enhances our understanding of the functional characteristics of the <em>GST</em> gene family in <em>T. ovatus</em> and provides new insights into its roles in antioxidative stress and immune response mechanisms.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"167 ","pages":"Article 105371"},"PeriodicalIF":2.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143892110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mx of Sebastes schlegelii: expression pattern, antibacterial activity and antiviral mechanism","authors":"Min Zhang ,&nbsp;Yue Wang ,&nbsp;Kai Yang ,&nbsp;Ziyue Chen ,&nbsp;Nuo Sun ,&nbsp;Guanghua Wang","doi":"10.1016/j.dci.2025.105374","DOIUrl":"10.1016/j.dci.2025.105374","url":null,"abstract":"<div><div>Myxovirus resistance (Mx) is a classical antiviral molecule that has been well understood in mammals. However, very limited studies on Mx antiviral activities have been documented in teleosts. In the present study, a novel Mx (SsMx) was cloned from black rockfish (<em>Sebastes schlegelii</em>) and the immunological activities of SsMx were examined <em>in vitro</em> and <em>in vivo</em>. SsMx contained conserved structural and functional domains including GTPase domain and GTPase effector domain. Quantitative real-time PCR (qRT-PCR) revealed that SsMx is extensively distributed in the immune cells and tissues examined with higher levels in spleen and liver. The mRNA expression of SsMx was significantly upregulated in head kidney, spleen and head kidney macrophages after pathogen infection. Recombinant SsMx (rSsMx) exhibited apparent binding activities against different bacteria <em>in vitro</em>. <em>In vivo</em> studies showed that rSsMx reduced pathogen dissemination and replication in head kidney and spleen. The subcellular localization results demonstrated that SsMx was predominantly distributed in the cytoplasm of transfected cells. Furthermore, SsMx was observed to inhibit apoptosis in virus-infected cells and reduce viral replication by interfering with the viral entry. SsMx and Spring viremia of carp virus Glycoprotein (SVCV G) were found to interact strongly with each other by co-immunoprecipitation and co-localization. These findings reveal an important role of SsMx in defencing the early stage of SVCV infection, which will be helpful to understand the molecular details of the antiviral mechanisms mediated by Mx proteins in teleosts.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"167 ","pages":"Article 105374"},"PeriodicalIF":2.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143886642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating transcriptomics and metabolomics revealed pathogenic mechanism of Chinese soft-shell turtle (Trionyx sinensis) infected with Trionyx sinensis hemorrhagic syndrome virus (TSHSV)","authors":"Weifeng Shen ,&nbsp;Shuang Zhao ,&nbsp;Sunjian Lyu ,&nbsp;Mingxing Zhang ,&nbsp;Qi Guo ,&nbsp;Bao Lou ,&nbsp;WenJun Ma ,&nbsp;Jingjing Zhan ,&nbsp;Li Liu ,&nbsp;Liang Li","doi":"10.1016/j.dci.2025.105373","DOIUrl":"10.1016/j.dci.2025.105373","url":null,"abstract":"<div><div><em>Trionyx sinensis</em> Hemorrhagic Syndrome Virus(TSHSV)seriously hinders the aquaculture of Chinese soft-shell turtle (<em>Trionyx sinensis</em>) due to its high mortality. However, the pathogenic mechanisms of TSHSV in <em>T. sinensis</em> are still unclear. In present study, transcriptomic and metabolomic analyses were performed on turtle livers following TSHSV infection. 734 up-regulated and 770 down-regulated differentially expressed genes (DEGs) were identified in different TSHSV challenge groups. These DEGs were categorized into 12 pathways related to virus infection and host immunity. Moreover, 27, 2679, and 4341 differentially expressed metabolites (DEMs) were identified in the D1, D3, and D5 groups, respectively. These DEMs were mapped into the pathways of energy metabolism, amino acid metabolism and fatty acid metabolism. Association analysis revealed TSHSV induced inflammatory responses, hepatocyte apoptosis, and ultimately led to liver tissue damage. Taurine supplementation promoted the survival rate of turtle after TSHSV infection and reduced the inflammatory response of liver by regulating the production of interferons, antioxidases, and the pro-inflammatory cytokine TNF-α. Collectively, our results provide comprehensive profiles of the transcriptome and metabolome in Chinese soft-shell turtle liver after TSHSV invasion, shedding light on the underlying pathogenic mechanism. The method of taurine supplementation might be a promising therapeutic strategy for protecting turtles from TSHSV.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"166 ","pages":"Article 105373"},"PeriodicalIF":2.7,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143854839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The class A scavenger receptor member 3 (SCARA3) regulates cell apoptosis through X-linked apoptosis inhibitory protein (XIAP) in turbot (Scophthalmus maximus L.)","authors":"Xinghua Zhuang ,&nbsp;Xuan Chen ,&nbsp;Lili Cao ,&nbsp;Beibei Wang ,&nbsp;Zhongyi Wang ,&nbsp;Suwan Li ,&nbsp;Honghong Li ,&nbsp;Chao Li ,&nbsp;Ning Yang","doi":"10.1016/j.dci.2025.105370","DOIUrl":"10.1016/j.dci.2025.105370","url":null,"abstract":"<div><div>Class A scavenger receptor 3 (SCARA3), a macrophage scavenger receptor-like protein, plays important roles in inhibiting cell proliferation, migration, and invasion. In the present study, a SCARA3 gene of turbot (<em>Sm</em>SCARA3) (Gene ID: 118289953) with an 1815 bp ORF encoding 604 amino acids was identified. Phylogenetic analysis revealed that <em>Sm</em>SCARA3 showed the closest relationship to that counterpart of olive flounder (<em>Paralichthys olivaceus</em>). The synteny analysis demonstrated conserved syntenic patterns across selected vertebrates. In addition, <em>Sm</em>SCARA3 was ubiquitously expressed in all the examined tissues, with the highest expression level in intestine and the lowest expression level in the brain. <em>Sm</em>SCARA3 exhibited different expression patterns in mucosal tissues (intestine, gill, skin) after two bacterial infections. Subsequently, recombinant <em>Sm</em>SCARA3 protein (r<em>Sm</em>SCARA3) revealed the strong binding affinity to LPS and responded primarily to LPS stimulation in intestinal cells of turbot. Additionally, the interference and overexpression experiments indicated that <em>Sm</em>SCARA3 was associated with apoptosis related genes, such as Caspase1, Caspase3 and Caspase3a, and it could activate Caspase3 in HEPG2 cells. Moreover, flow cytometry revealed the apoptosis of <em>Sm</em>SCARA3 overexpression group increased by 10.03%, which was consistent with the effect of <em>Sm</em>SCARA3 on proliferation inhibition in intestinal cells of turbot. The cell apoptosis levels in the <em>Sm</em>SCARA3-Flag and XIAP-HA experimental group were significantly lower than that in the control group (51.17% vs 72.72%). Finally, the Co-IP assay showed that <em>Sm</em>SCARA3 could directly interact with XIAP. In conclusion, our results indicated that <em>Sm</em>SCARA3 could activate Caspase3 and modulate apoptosis through XIAP , highlighting its potential roles as a therapeutic target for fish diseases.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"166 ","pages":"Article 105370"},"PeriodicalIF":2.7,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143802725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EXOC8 of Epinephelus coioides involved in SGIV infection via innate immunity and apoptosis","authors":"Wei Huang ,&nbsp;Zi-An Chen ,&nbsp;Qi-Yin Li ,&nbsp;Cui-Fen Huang ,&nbsp;Yun-Xiang Lin ,&nbsp;Yin-Mei Lan ,&nbsp;Ze-Peng Zhang ,&nbsp;Yu-Feng Jiang ,&nbsp;Qi-Wei Qin ,&nbsp;Hong-Yan Sun","doi":"10.1016/j.dci.2025.105368","DOIUrl":"10.1016/j.dci.2025.105368","url":null,"abstract":"<div><div>The exocyst complex (EXOC) plays a major role in the extracellular secretion of organisms. In this study, EXOC8, a member of the EXOC family, was characterized from <em>Epinephelus coioides</em>,an important economical important fish in southern China and Southeast Asia, and its role response to viral infection was explored. The full length of <em>E</em>. <em>coioides</em> EXOC8 is 3091 bp including a 2061 bp open reading frame (ORF) encoding 686 amino acids, with a molecular mass of 79037.42 Da. The mRNA of <em>E</em>. <em>coioides</em> EXOC8 can be detected in all of the tissues examined with different levels. <em>E</em>. <em>coioides</em> EXOC8 is distributed in the cytoplasm. The expression of <em>E</em>. <em>coioides</em> EXOC8 was up-regulated during Singapore grouper iridovirus (SGIV) infection, an important pathogen of <em>E</em>. <em>coioides</em>. Overexpressing <em>E</em>. <em>coioides</em> EXOC8 significantly promoted the formation of cytopathic effects (CPE) caused by SGIV infection and the expressions of SGIV key genes MCP, VP19, LITAF and ICP18; but significantly inhibited the activities of NF-κB/AP-1 promoter, apoptosis induced by SGIV, and the expressions of inflammatory factors (IL-6,IL-8, IL-1<em>β</em> and TNF-α) in <em>E. coioides</em>. The results demonstrated that <em>E. coioides</em> EXOC8 may be involved in SGIV infection, providing a theoretical basis for clearing the mechanisms of viral infection in fish.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"166 ","pages":"Article 105368"},"PeriodicalIF":2.7,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143794548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ToIκB and ToIKK genes from golden pompano (Trachinotus ovatus): Molecular characterization, expression, and association with tolerance to Streptococcus agalactiae infection","authors":"Jie Gao ,&nbsp;Ming-Jian Liu ,&nbsp;Jin-Min Pan ,&nbsp;Hua-Yang Guo ,&nbsp;Bao-Suo Liu ,&nbsp;Ke-Cheng Zhu ,&nbsp;Nan Zhang ,&nbsp;Dian-Chang Zhang","doi":"10.1016/j.dci.2025.105369","DOIUrl":"10.1016/j.dci.2025.105369","url":null,"abstract":"<div><div>Effective disease management is crucial for sustainable aquaculture, particularly for economically important species like golden pompano (<em>Trachinotus ovatus</em>). <em>Streptococcus agalactiae</em> represents a major threat to this species, leading to severe health issues and significant economic losses. Understanding the immune mechanisms involved is essential to address this challenge. The <em>IκB</em> and <em>IKK</em> genes are known to be key regulators of immune responses, playing pivotal roles in modulating inflammatory pathways during infections. However, their specific roles in golden pompano immunity are not well characterized. In this study, we used bioinformatics analysis and tissue-specific expression profiling to investigate the roles of <em>IκB</em> and <em>IKK</em> genes in golden pompano during bacterial infection. The results demonstrated that <em>ToIKK</em> was significantly upregulated during the early stages of infection, indicating rapid immune activation, while <em>ToIκB</em> showed an initial decrease followed by recovery, suggesting its involvement in inflammation modulation. These genes were found to regulate the NF-κB signaling pathway, which is crucial for coordinating the immune response to bacterial infection. This research provides valuable insights into the molecular basis of golden pompano immune response against <em>S. agalactiae</em>, offering a foundation for developing targeted anti-infection strategies and improving disease resistance and health management practices in aquaculture.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"166 ","pages":"Article 105369"},"PeriodicalIF":2.7,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143786024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The molecular mechanism of ferroptosis in the Pacific oyster Crassostrea gigas under Erastin treatment or high temperature stress","authors":"Jinyuan Leng ,&nbsp;Jiejie Sun ,&nbsp;Zhicheng Guo ,&nbsp;Lingling Wang ,&nbsp;Linsheng Song","doi":"10.1016/j.dci.2025.105366","DOIUrl":"10.1016/j.dci.2025.105366","url":null,"abstract":"<div><div>Ferroptosis is an iron- and lipotoxicity-dependent form of programmed cell death, and it is distinct from apoptosis, pyroptosis, and autophagy. In the present study, the hemocytes were found to be shrunken under Erastin treatment or high temperature stress. The mitochondrial atrophy, crest loss and fracture were observed in hemocytes under high temperature stress. In addition, the fluorescence intensity of mitochondrial probe JC-1 monomers increased significantly in hemocytes under high temperature stress. Hemocytes were found to be wrinkled under ultrastructure and the contents of LPO, ROS and GSH increased significantly under Erastin treatment or high temperature stress. The band intensity of <em>Cg</em>VDAC2 also decreased under Erastin treatment or high temperature stress. The mRNA expressions of genes involved in enhancing the antioxidation system as well as genes involved in promoting the iron metabolism all decreased significantly under Erastin treatment or high temperature stress. Those of genes involved in impairing the antioxidation system, genes involved in inhibiting the iron metabolism, as well as genes involved in reducing the lipid peroxidation all increased significantly under Erastin treatment or high temperature stress. These results indicated that Erastin could activate the three key ferroptotic signaling pathways in oyster and the activation mechanism of ferroptosis in oyster under high temperature stress was similar with that under Erastin treatment.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"166 ","pages":"Article 105366"},"PeriodicalIF":2.7,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143788026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Allogenic grafting induces PI3K-mediated tissue overgrowth in hydrozoan jellyfish Cladonema radiatum medusae.","authors":"Crystal Tang, Miwa Tamura-Nakano, Kazunori Tachibana","doi":"10.1016/j.dci.2025.105367","DOIUrl":"https://doi.org/10.1016/j.dci.2025.105367","url":null,"abstract":"<p><p>Allorecognition, which is the ability of an organism to discriminate between self and non-self, protects multicellular animals from somatic cell/germline parasitism. We reported and characterised allorecognition in the stolons of colonies of the hydrozoan jellyfish Cladonema radiatum (C. radiatum) in our previous publication. C. radiatum has a free-swimming medusa form besides the sessile colonial form. In this study, we investigated the allorecognition responses in the medusa form of C. radiatum. By using grafting experiments, we observed that while C. radiatum medusae show tolerance to both isogenic and allogenic chimerism, allogenic grafting induces the formation of a form of circular scars-we refer to as \"ring-shaped scars\"-around the grafts on the host umbrella. Within the scars, overgrowth of tissues occurs with additional gastrovascular canal development. By pharmaceutical experiments, we found that tissue overgrowth is dependent on phosphoinositide 3-kinase (PI3K) and vascular endothelial growth factor (VEGF), showing a resemblance to mammalian neoplasia.</p>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":" ","pages":"105367"},"PeriodicalIF":2.7,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143788022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FK506 activates the BMP signaling pathway to regulate ovarian development in Portunus trituberculatus","authors":"Xiaocong Chen ,&nbsp;Ce Shi ,&nbsp;Yangfang Ye ,&nbsp;Chunlin Wang ,&nbsp;Ronghua Li ,&nbsp;Huan Wang ,&nbsp;Congcong Hou ,&nbsp;Weiwei Song ,&nbsp;Changkao Mu","doi":"10.1016/j.dci.2025.105365","DOIUrl":"10.1016/j.dci.2025.105365","url":null,"abstract":"<div><div>Bone morphogenic proteins (BMPs) play important regulatory roles in the development of follicles in mammals. However, studies on the roles of BMPs in ovarian development in low-level aquatic animals, especially the swimming crab <em>Portunus trituberculatus</em>, are limited. In this study, a BMP Ⅰ-type receptor-specific activator (tacrolimus, FK506) was administered at different concentrations via in vivo injection, and the effects of FK506 on the regulation of the BMP signaling pathway during ovarian development in <em>P</em>. <em>trituberculatus</em> were examined. The tissue and cell morphology was observed, and a combined transcriptomics, proteomics and metabolomics analysis was carried out. Crabs administered FK506 exhibited elevated GSI alongside reduced HSI compared to control and blank groups. The main biological processes enriched by joint analysis included lipid metabolism, sugar metabolism, and amino acid metabolism. Fatty acid composition analysis revealed that the activator may activate the BMP signaling pathway to promote ovarian development and accelerate the transport of unsaturated fatty acids from the hepatopancreas to the ovaries. Amino acid metabolism and carbohydrate metabolism provide transporter proteins and energy for lipid metabolism. This study is highly important because it reveals the molecular mechanism by which the BMP signaling pathway regulates gonadal development in a crustacean.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"166 ","pages":"Article 105365"},"PeriodicalIF":2.7,"publicationDate":"2025-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143763309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}