Developmental and comparative immunology最新文献

{"title":"Characterization and functional analysis of interleukin-6 and its receptor subunits (IL-6Rα and IL-6Rβ) in the yellow drum, Nibea alibiflora.","authors":"Jiaxin Liu, Mingming Wenren, Xu Zhou, Dongdong Xu, Changfeng Chi, Zhenming Lü, Huihui Liu","doi":"10.1016/j.dci.2024.105305","DOIUrl":"https://doi.org/10.1016/j.dci.2024.105305","url":null,"abstract":"<p><p>Interleukin 6 (IL-6) is one of the cytokines found to be multifunctional and biologically effective, regulating immune and inflammatory response by interacting with receptors to transmit signals. In this study, the full-length cDNAs of IL-6 (named as NaIL-6) and its receptors IL-6R and gp130 (named as NaIL-6Rα and NaIL-6Rβ) of Nibea albiflora were acquired and they possessed the typical symbolic motifs similar to its teleost orthologues in multiple sequence comparisons. The phylogenetic trees showed that NaIL-6 and its receptors clustered with their counterparts in bony fish, and had the closest affinity to Larimichthys crocea. Real-time PCR indicated that NaIL-6, NaIL-6Rα and NaIL-6Rβ were widely expressed in different tissues, among which NaIL-6 was highly expressed in the liver, NaIL-6Rα showed the highest expression in the kidney and NaIL-6Rβ was reflected in the liver. Following stimulation by Vibrio parahaemolyticus, Vibrio alginolyticus, or Polyinosinic-polycytidylic acid (Poly (I:C)) infection, the mRNA expression of all three genes were greatly up-regulated over time. The cell localization analysis showed that NaIL-6 distributed in cytoplasm and cell membrane, and NaIL-6Rα located on the cell membrane. After co-transfection of NaIL-6- mCherry and NaIL-6Rα-EGFP, they co-expressed as orange at the same position for their possibility of spatial interactions on the cellular membrane. By GST-Pull down with the purified target proteins, the association between NaIL-6-His and NaIL-6Rα-GST was confirmed, which may be utilized for further functional study. Taken together, the results indicated that the biological functions of NaIL-6 and its receptors NaIL-6Rα and NaIL-6Rβ involved in the immunologic mechanism in N.albiflora, which would provide a basis for the research of immune functions and signaling pathway of IL-6 and its receptors in fish.</p>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":" ","pages":"105305"},"PeriodicalIF":2.7,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142881529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CgmiR307 involved in the regulation of Nrf2-dependent oxidative response in the Pacific oyster Crassostrea gigas under high-temperature stress.","authors":"Keli Zhou, Lei Gao, Pingan Ge, Ling Wang, Lu Liu, Jiayu Ye, Hairu Xu, Lingling Wang, Linsheng Song","doi":"10.1016/j.dci.2024.105306","DOIUrl":"https://doi.org/10.1016/j.dci.2024.105306","url":null,"abstract":"<p><p>miRNA, a type of endogenous small non-coding RNA, is involved in the response to various environmental stresses through post-transcriptional regulation. In the present study, the role of CgmiR307 in the regulation of oxidative response under high-temperature stress by targeting CgNrf2 was investigated in the Pacific oyster Crassostrea gigas. The binding site of CgmiR307 were predicted at 1,799-1,818 bp in the 3'-UTR of CgNrf2, and the binding activity of CgmiR307 with the mRNA of CgNrf2 was further proved by the dual-luciferase reporter assay. The expression levels of CgmiR307 and CgNrf2 in gill were significantly higher than in other tissues, and exhibited significant fluctuations and variations after exposure to 28°C. There was a significant reduction in the expression levels of CgSOD, and CgCAT in gill, as well as the activities of SOD, CAT, and T-AOC, while ROS and MDA contents significantly increased in CgNrf2-RNAi oysters. After CgmiR307 agomir injection and high-temperature stress, the expression levels of CgNrf2, CgSOD and CgCAT in gill, the activities of SOD and CAT and T-AOC decreased significantly, while ROS and MDA content significantly increased. After CgmiR307 antagomir injection and high-temperature stress, the changes in the parameters of oxidative response shown exactly the opposite trend. These results demonstrated that CgmiR307 was involved in the regulation of oxidative response by inhibiting the mRNA expression of CgNrf2 under high-temperature stress.</p>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":" ","pages":"105306"},"PeriodicalIF":2.7,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142876537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel digestive protease chymotrypsin-like serine contributes to anti-BmNPV activity in silkworm (Bombyxmori).","authors":"Wei-Wei Kong, Yu-Liang Yan, Cai-Ping Hou, Tao Hong, Yi-Sheng Wang, Xin Xu, Shi-Huo Liu, Jia-Ping Xu","doi":"10.1016/j.dci.2024.105301","DOIUrl":"10.1016/j.dci.2024.105301","url":null,"abstract":"<p><p>Serine proteases (SPs) are important proteases in the digestive system of lepidopteran insects. They play important roles in protein digestion, coagulation, signal transduction, hormone activation, inflammation and development. Blood-borne pyosis caused by Bombyx mori nuclear polyhedrosis virus (BmNPV) has caused serious harm to sericulture. At present, the scientific problems of BmNPV infection and silkworm resistance to BmNPV infection have been the focus of many scientists, but the molecular mechanism needs further research and exploration. Based on the results of label-free quantitative protein proteomics of the midgut digestive juice of different resistant strains in our laboratory, we successfully screened a differentially expressed candidate protein (DEP), B. mori chymotrypsin-like serine protease (BmCLSP), and comprehensively analyzed the biological characteristics and anti-BmNPV function of BmCLSP. The open reading frame (ORF) of BmCLSP is 891 bp, encoding 296 amino acid residues. The analysis of the domain structure showed that there was a signal peptide and a trypsin-like serine protease domain, Tryp_SPC, in the BmCLSP protein. Semi-quantitative and real-time fluorescence quantitative PCR analysis showed that the BmCLSP gene was highly expressed in the fifth instar larvae of silkworm, and specifically expressed in the midgut. The expression level of BmCLSP in the BmNPV resistant strain A35 was higher than that in the sensitive strain P50. Virus amplification analysis showed that the relative expression level of VP39 was significantly lower than that of the control group after infection of silkworm larvae and BmN cells with BmNPV treated with recombinant BmCLSP at an appropriate concentration. Furthermore, our overexpression of BmCLSP in BmN cells significantly inhibited the expansion of BmNPV. In summary, the results of this study indicate that BmCLSP has anti-BmNPV activity in silkworm, and can significantly inhibit the proliferation of BmNPV in silkworm. It offers a promising avenue for silkworm anti-virus breeding.</p>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":" ","pages":"105301"},"PeriodicalIF":2.7,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142823987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Haemolymphatic tissues of captive boa constrictor (Boa constrictor): morphological features in healthy individuals and with Boid Inclusion Body Disease.","authors":"E Dervas, E Michalopoulou, J Hepojoki, T Thiele, F Baggio, U Hetzel, A Kipar","doi":"10.1016/j.dci.2024.105302","DOIUrl":"https://doi.org/10.1016/j.dci.2024.105302","url":null,"abstract":"<p><p>Knowledge on the structure and composition of the haematopoietic tissue (HT) is essential to understand the basic immune functions of the immune system in any species. For reptiles, it is extremely limited, hence we undertook an in-depth in situ investigation of the HT (bone marrow, thymus, spleen, lymphatic tissue of the alimentary tract) in the common boa (Boa constrictor). We also assessed age- and disease-related changes, with a special focus on Boid Inclusion Body Disease , a highly relevant reptarenavirus-associated disease in boid snakes. The HT was subjected to gross, histological and ultrastructural examination, including special stains to highlight collagen and reticulin fibers and iron, immunohistochemistry, in situ hybridization and morphometric analyses. In general, the HT was dominated by T cells and lacked a clear structural organization, such as follicle formation. BIBD was associated with significantly higher cellularity and a granulomatous response in the spleen, and the presence of virus-infected haematopoietic cells in the bone marrow, suggesting the latter as a persistent source of viremia.</p>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":" ","pages":"105302"},"PeriodicalIF":2.7,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142853501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the immunological functions of thioredoxin domain-containing protein 17 (TXNDC17) in chub mackerel (Scomber japonicus): Immune response and cellular redox homeostasis.","authors":"Arthika Kalaichelvan, Jeongeun Kim, Gaeun Kim, Ji Hun Lee, H M V Udayantha, Y K Kodagoda, W A D L R Warnakula, G A N P Ganepola, Yuhwan Jo, U P E Arachchi, B P M Vileka Jayamali, Qiang Wan, Sumi Jung, Jehee Lee","doi":"10.1016/j.dci.2024.105303","DOIUrl":"10.1016/j.dci.2024.105303","url":null,"abstract":"<p><p>All organisms have evolved sophisticated antioxidant networks and enzymes to counteract reactive radicals, among which thioredoxin (Trx) systems are especially noteworthy. Thioredoxin domain-containing protein 17 (TXNDC17) is a ubiquitously expressed enzyme with oxidoreductase activity belonging to the Trx protein family. This study successfully uncovered and analyzed the TXNDC17 gene in Scomber japonicus (SjTXNDC17). The gene consists of a 372-base-pair coding sequence that encodes a protein of 123 amino acids, with an estimated molecular weight of 14.1 kDa. Structural analysis revealed that SjTXNDC17 contains a TRX-related protein 14 domain with two redox-responsive cysteine residues in the ⁴²WCPDC⁴⁶ motif. Spatial expression analysis indicated that SjTXNDC17 had the highest constitutive expression in the brain. Stimulation with polyinosinic-polycytidylic acid (poly I:C), Vibrio harveyi, and Streptococcus iniae, significantly upregulated the mRNA levels of SjTXNDC17 in the head kidney. The antioxidant activity of the recombinant SjTXNDC17 protein was evidenced by 2,2-Diphenyl-1-picryl-hydrazyl-hydrate (DPPH) radical scavenging, insulin reduction, and cupric ion-reducing antioxidant capacity assays. SjTXNDC17 overexpression in fathead minnow (FHM) cells significantly reduced reactive oxygen species (ROS) levels and decreased apoptosis. The anti-apoptotic effect was driven by the upregulation of the Bcl2 gene and the downregulation of the Bax gene, as well as the suppression of JNK signaling pathway genes. Moreover, overexpression of SjTXNDC17 facilitated M2 polarization and suppressed nitric oxide production in macrophages. Collectively, these results demonstrate that SjTXNDC17 plays a crucial role in both the immune response and cellular redox balance in Scomber japonicus.</p>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":" ","pages":"105303"},"PeriodicalIF":2.7,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142827908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of FcμR by bovine mononuclear blood leukocytes.","authors":"Florence B Gilbert, Rodrigo P Martins, Pascal Rainard","doi":"10.1016/j.dci.2024.105304","DOIUrl":"10.1016/j.dci.2024.105304","url":null,"abstract":"<p><p>The receptor for IgM has been identified a few years ago, but its expression by bovine mononuclear cells has not yet been studied. We used rabbit antibodies against bovine FcμR to begin to fill this gap. Anti-FcμR antibodies bound to B lymphocytes and monocytes, although less than to neutrophils. Nonclassical and intermediate monocytes (CD172a^pos CD16^pos) bound nonspecifically to rabbit antibodies, complicating analysis, but they bound more anti-FcμR antibodies than control antibodies, indicating that they also express the FcμR. They also express more C5a receptors than classical monocytes. Anti-FcμR antibodies did not bind to CD3^pos αβT lymphocytes (both CD4^pos and CD8^pos) and γδT cells. At low temperature but not at physiological temperature, purified bovine IgM bound to all monocytes and strongly to all B cells, but hardly to CD3^pos T cells. Monocytes and B cells bound human IgA, but IgA did not compete, whereas unlabeled bovine IgM competed for binding of labeled IgM. This supports the role of the FcμR, and not the FαμR, in IgM binding. Finally, we showed that monocytes were able to ingest bacteria opsonized with serum deprived of IgG, indicating their ability to perform IgM-dependent phagocytosis. In conclusion, surface expression of FcμR by unstimulated blood leukocytes was demonstrated on B cells and monocytes, but not on T cells.</p>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":" ","pages":"105304"},"PeriodicalIF":2.7,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142823989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HSC70 functions as a negatively regulator in IFN signaling pathway via suppressing K63-linked ubiquitination of RIG-I in black carp.","authors":"Jiaxin Fu, Nianfeng Chen, Tian Qin, Yixin Chen, Ji Liu, Hui Wu, Jun Yan, Jun Xiao, Jun Zou, Hao Feng","doi":"10.1016/j.dci.2024.105300","DOIUrl":"10.1016/j.dci.2024.105300","url":null,"abstract":"<p><p>Heat shock cognate 70 (HSC70), a highly conserved molecular chaperone in the heat shock protein 70 (HSP70) family, plays an essential role in maintaining the homeostasis of the cellular environment. Furthermore, although previous studies have investigated potential function of HSC70 in innate antiviral immunity, further research is still required to fully elucidate its role. In this study, we cloned and characterized the HSC70 homolog gene from black carp (Mylopharyngodon piceus), which consists of 1950 nucleotides encoding 650 amino acids, migrates at approximately 71 kDa on SDS-PAGE, and is distributed in the cytoplasm. In response to different stimuli (SVCV, poly (I:C) and LPS), the transcription level of black carp HSC70 (bcHSC70) all increased to a certain extent. Luciferase reporter assay demonstrated that co-transfected bcHSC70 obviously reduced activity of interferon (IFN) promoters mediated by most factors in the RLRs pathway, and further qRT-PCR and plaque assay indicated that co-transfection of bcHSC70 with bcRIG-I decreased the bcRIG-I-mediated IFN transcription and antiviral ability resisting spring viremia of carp virus (SVCV), whereas knockdown of bcHSC70 improves the host cellular antiviral activity. Noteworthily, co-immunoprecipitation (co-IP) assay and immunofluorescence (IF) assay confirmed bcHSC70 interacts with bcRIG-I, and weaken K63-linked polyubiquitination of bcRIG-I. In summary, our study revealed that HSC70 negatively regulates IFN signaling pathway through impairing K63-linked ubiquitination of RIG-I in black carp, which provides an important basis for exploring innate immune regulatory mechanisms in teleost fish.</p>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":" ","pages":"105300"},"PeriodicalIF":2.7,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142794190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomics reveals crowding stress inhibit the immune defense of the head kidney of the pearl gentian grouper juvenile through NF-κB signal pathway.","authors":"Wenyang Li, Ao Li, Xianhong Zhang, Fan Fei, Xiaoqiang Gao, Yingying Fang, Shuquan Cao, Hongjun Yang, Wensheng Li, Baoliang Liu","doi":"10.1016/j.dci.2024.105299","DOIUrl":"10.1016/j.dci.2024.105299","url":null,"abstract":"<p><p>Crowding stress is a significant welfare factor affecting aquatic animals in recirculating aquaculture systems. Little is known regarding the influence of prolonged crowding stress on the immunity of juvenile pearl gentian groupers. However, research exploring the potential mechanisms through which crowding stress affects fish immune function is limited. Therefore, this study aims to investigate the effect of crowding stress on the immune stress of the pearl gentian grouper juvenile (♀Epinephelus fuscoguttatus × ♂ Epinephelus lanceolatus) under prolonged conditions. We focused on the pearl gentian grouper juvenile and selected low- and high-density groups as the experimental breeding densities. Research shows that crowding stress increases the activities of alkaline acid plum and acid phosphatase, reduces the activities of lysozyme and immunoglobulin M content. RNA sequencing and comparative transcriptomic analyses were employed to explore changes in the gene expression of juvenile pearl gentian groupers subjected to crowding stress. Differential gene expression analyses between the low- and high-density groups identified 5777 unigenes that were differentially expressed following crowding stress, with 3216 and 2561 upregulated and downregulated, respectively. In the GO and KEGG enrichment analyses, many of the enriched signaling pathways related to genes were associated with immunity and oxidative stress. In addition, the combined analyses of enzyme activity and transcriptomics indicated that crowding stress suppressed the immune function of juvenile pearl gentian groupers, reducing their immune ability. Overall, these findings offer new insights into the molecular mechanisms underlying crowding stress tolerance in juvenile pearl gentian grouper, suggesting that the NF-κB pathway plays a crucial role in the immune response of the head kidney of the pearl gentian grouper to long-term crowding stress.</p>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":" ","pages":"105299"},"PeriodicalIF":2.7,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142791303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ontogeny of the organized nasopharynx-associated lymphoid tissue in rainbow trout.","authors":"Benjamin J Garcia, Narmin Musayeva, Alexis Reyes, Chrysler Martinez, Yago Serra Dos Santos, Irene Salinas","doi":"10.1016/j.dci.2024.105298","DOIUrl":"10.1016/j.dci.2024.105298","url":null,"abstract":"<p><p>Understanding the ontogeny of teleost mucosa-associated lymphoid tissues (MALT) is critical for determining the earliest timepoint for effective mucosal vaccination of young fish. Here, we describe the developmental sequence that leads to the formation of an organized MALT structure in rainbow trout, the organized nasopharynx-associated lymphoid tissue (O-NALT). Control rainbow trout were sampled between 340 and 1860 degree days (DD) and routine histology and immunofluorescence staining were used to determine cellular changes in immune cells in the nasal cavity as well as O-NALT formation. We identified that O-NALT is first seeded by CD8α⁺ T cells at 700 DD followed by IgM⁺ B cells and cd4-2b⁺ cells at 1000 DD. Histomorphologically, trout O-NALT is fully formed at 1400 DD. Whole body gene expression analyses uncovered waves of igmh, cd4-2b, and cd8a expression that recapitulate the cellular seeding sequence of O-NALT by specific lymphocyte subsets. Our results indicate that 1) O-NALT formation results from a specific sequence of lymphocyte subset colonization pioneered by CD8α⁺ T cells and 2) the presence of the full O-NALT structure at 1400 DD may mark this timepoint as the earliest developmental stage at which mucosal vaccines can induce long lasting, specific immune responses.</p>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":" ","pages":"105298"},"PeriodicalIF":2.7,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142791298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular dynamics and spatial response of proliferation and apoptosis in wound healing and early intestinal regeneration of sea cucumber Apostichopusjaponicus.","authors":"Fang Su, Igor Yu Dolmatov, Wei Cui, Hongsheng Yang, Lina Sun","doi":"10.1016/j.dci.2024.105297","DOIUrl":"10.1016/j.dci.2024.105297","url":null,"abstract":"<p><p>The sea cucumber, Apostichopus japonicus, exhibits significant regenerative capabilities. To ensure survival and reduce metabolic costs under adverse conditions, A. japonicus can expel intestine, respiratory trees and other internal organs. It takes only 14 days to regenerate a fully connected, lumen-containing intestine. Despite numerous reports characterizing the cellular events in intestinal regeneration, limited investigation has been conducted on the molecular events that occur during wound healing and the initial stages of regeneration after evisceration. Here, we identified differentially expressed genes (DEGs) during wound healing (6 h post-evisceration, Aj6hpe) and early intestinal regeneration (Aj1dpe, Aj3dpe, Aj7dpe). Cell proliferation and apoptosis were detected by EdU and TUNEL assays, respectively. Results demonstrated that calcium ion and neuroactive ligand-receptor interaction were involved in the transmission of injury signals from evisceration to Aj1dpe. The main events occurring in the wound healing and early regeneration process were autophagy, apoptosis, dedifferentiation, migration and shutdown of feeding. Cell proliferation was primarily observed during the lumen formation stage. Maximal number of apoptotic cells were found during wound healing stage (6 hpe - 1 dpe). Consequently, the immune response is mainly mobilized by neural regulation after evisceration. Our findings bridge the gap between evisceration and regeneration, illuminating the molecular events that mediate damage response and initiate regeneration. This study significantly advances our understanding of the mechanisms underlying intestinal regeneration.</p>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":" ","pages":"105297"},"PeriodicalIF":2.7,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142783711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}