Developmental and comparative immunology最新文献

{"title":"The Wnt/β-catenin signalling pathway regulates ovarian development in the swimming crab Portunus trituberculatus","authors":"Xiaona Zhang ,&nbsp;Huan Wang ,&nbsp;Lei Liu ,&nbsp;Ronghua Li ,&nbsp;Yangfang Ye ,&nbsp;Weiwei Song ,&nbsp;Ce Shi ,&nbsp;Chunlin Wang ,&nbsp;Changkao Mu","doi":"10.1016/j.dci.2025.105492","DOIUrl":"10.1016/j.dci.2025.105492","url":null,"abstract":"<div><div>Wnt/β-catenin signalling regulates many physiological processes in organisms, including development and tissue homeostasis maintenance. Signal transduction in this pathway involves the regulation of its multiprotein complex, which targets β-catenin for proteasomal degradation. The genes encoding the cytoplasmic regulatory proteins β-catenin and GSK-3β in <em>Portunus trituberculatus</em> were successfully cloned and characterized here. <em>Ptβ-catenin</em> was most highly expressed in hemocytes, <em>PtGSK-3β</em> in ovarian tissue, and both genes were significantly upregulated at ovarian stage III, as revealed by quantitative real-time RT-PCR analysis. Continuous injection of the Wnt/β-catenin signalling activator CHIR-98014 resulted in decreased expression of <em>PtGSK-3β</em>, increased expression of <em>Ptβ-catenin</em>, decreased expression of <em>PtVg</em> (vitellogenin) and the content of r<em>PtVn</em> (vitellin). Additionally, it resulted in a significant reduction in both gonadosomatic index (GSI) and oocyte diameter. HE staining revealed diminished vitellogenic granules and the formation of cytoplasmic vacuoles in the oocytes. By contrast, continuous injection ETC-159, a potent inhibitor of Wnt/β-catenin signalling caused increased expression of <em>PtGSK-3β</em>, decreased expression of <em>Ptβ-catenin</em>, increased expression of <em>PtVg</em> and the content of r<em>PtVn</em>. Furthermore, it resulted in an increased GSI, larger oocyte diameter, and HE staining revealed a greater number of yolk granules and a tight arrangement of oocytes. This study elucidates the critical regulatory function of Wnt/β-catenin signaling in <em>P. trituberculatus</em> ovarian development, establishing a foundation for future investigations into the molecular mechanisms governing crustacean reproductive physiology.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"172 ","pages":"Article 105492"},"PeriodicalIF":2.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145307041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peripheral blood mononuclear cell-driven cytokine and transcription factors induction confers resistance to Haemonchus contortus in Black Bengal goats","authors":"Nurnabi Ahmed ,&nbsp;Babul Chandra Roy ,&nbsp;Amitav Biswas ,&nbsp;Md Rajiur Rahaman Rabbi ,&nbsp;Md Mahfuzur Rahman Sajib ,&nbsp;Mohammad Manjurul Hasan ,&nbsp;Hiranmoy Biswas ,&nbsp;MD Hasanuzzaman Talukder","doi":"10.1016/j.dci.2025.105470","DOIUrl":"10.1016/j.dci.2025.105470","url":null,"abstract":"<div><div><em>Haemonchus contortus</em> is a major threat to small ruminant health and productivity. Although early Th2 cytokine and transcription factor expression confers protection in naturally resistant breeds, the immune basis of resistance in Black Bengal goats (BBG) remains unexplored. We compare early PBMC-mediated cytokine and transcriptional responses and their direct effects on larval motility between naive and primed BBG kids during the first seven days of infection. Kids were primed with 2000 L₃ weekly for four weeks or left naive, then challenged with 10,000 L₃. Two kids per group were sacrificed on each time point. Whole blood was collected pre-mortem for differential counts and PBMC isolation, while abomasal mucosa and draining LN were harvested for histology and RNA extraction. Primed kids exhibited a 58 % reduction in abomasal L₄ burden by day 7, elevated PCV (<em>p &lt;</em> 0.05), and a threefold greater increase in LN weight compared to naive kids. Histopathology revealed significantly enhanced eosinophil and neutrophil infiltration in abomasal mucosa of primed kids. Cytokine and gene expression assay showed early upregulation of Interleukin (IL)-4, IL-5, IL-13, IL-33, MCP-1, CXCL-1, TLR-2, and GAL-14 (p &lt; 0.05). <em>In vitro</em>, co-culture with primed PBMCs reduced L₃ motility compared to naive PBMCs (p &lt; 0.01) and L₃ pretreated with primed PBMCs resulted in a 60 % reduction in fecal egg counts by week 5 (p &lt; 0.001). This is the first study to integrate daily PBMC transcriptomics with functional motility and infectivity assays in BBGs. The findings identify novel biomarkers, inform selective breeding and immunoprophylactic strategies for sustainable parasite control.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"172 ","pages":"Article 105470"},"PeriodicalIF":2.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145102552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A flow cytometric approach to identifying the relative abundance and functional capacities of hemocyte subsets in the American cockroach, Periplaneta americana (L.)","authors":"Faith J. Boyer-Millander ,&nbsp;Aaron T. Martin ,&nbsp;Chadwick A. Hamm ,&nbsp;Arthur G. Appel ,&nbsp;Elizabeth Hiltbold Schwartz","doi":"10.1016/j.dci.2025.105508","DOIUrl":"10.1016/j.dci.2025.105508","url":null,"abstract":"<div><div>There is growing interest in insects as subjects for comparative immunological studies; however, very little has been done to quantitatively characterize insect immune cells using modern techniques such as flow cytometry, and virtually no work of this kind has been done in <em>Periplaneta americana</em>. Here, we use an array of general molecular probes including fluorescent lectins, lysosomal indicators, and functional assays to distinguish and characterize insect immune cells (hemocytes) based on cell markers and functions. We have utilized fluorescent tracers of lysosomal content, ROS production, and phagocytosis, as well as microscopic examination of morphology and melanization to distinguish hemocyte types based on these functions. Our findings support the use of lectins as an additional means of separating at least three populations of cockroach immune cells coupled with morphological measurements such as size and complexity. Our results indicate that many functions are enriched in the more granular hemocyte population as these are the cells that exhibit phagocytosis and melanization.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"172 ","pages":"Article 105508"},"PeriodicalIF":2.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145451150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression and characterization of activation-induced deaminase (AID) with dehydroaminase activity and its localization in IgM+ lymphocytes of spleen of common carp (Cyprinus carpio)","authors":"Guangcai Wei ,&nbsp;Jianhui Zhao ,&nbsp;Qingyun Zuo ,&nbsp;Manting Sun ,&nbsp;Hua Wei ,&nbsp;Haofeng Wang ,&nbsp;Guiwen Yang ,&nbsp;Eakapol Wangkahart ,&nbsp;Lei Wang ,&nbsp;Fumiao Zhang","doi":"10.1016/j.dci.2025.105489","DOIUrl":"10.1016/j.dci.2025.105489","url":null,"abstract":"<div><div>Activation-induced deaminase (AID) is a key enzyme that catalyzes the deamination of cytosine to uracil in DNA, driving somatic hypermutation (SHM) and class switch recombination (CSR) of antibody genes to promote antibody diversification in the adaptive immunity. The AID gene from <em>Cyprinus carpio</em> (CcAID) has a full-length open reading frame of 633 bp and encodes a protein of 210 amino acids. CcAID shares structural and functional similarities with apolipoprotein B mRNA-editing enzyme, catalytic polypeptide-like (APOBEC) family members and contains a conserved catalytic domain characteristic of cytidine deaminases. Notably, the absence of two key phosphorylation sites in CcAID (Ser38 and Tyr184) may be a significant factor contributing to the markedly lower levels of somatic hypermutation (SHM) in teleost fish. A high level of CcAID transcript expression was detected in carp larvae on the sixth day after fertilization. Intraperitoneal injection of T-independent and T-dependent antigens preferentially upregulated CcAID expression in head kidney and splenic lymphocytes. Immunofluorescence analysis confirmed its expression in IgM⁺ spleen lymphocytes. Recombinant CcAID exhibited cytidine deaminase activity. These findings indicate that CcAID is specifically expressed in IgM⁺ spleen lymphocytes, suggesting its potential role in antibody production.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"172 ","pages":"Article 105489"},"PeriodicalIF":2.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145279227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elucidating the regulatory role of Nrf2 in Beclin1-mediated autophagy in freshwater bivalve Hyriopsis cumingii","authors":"Yuzhuo He,&nbsp;Shaoyu Hu,&nbsp;Qinglin Yang,&nbsp;Xiaobo Yu,&nbsp;Yanhong Li,&nbsp;Zhengli Wu","doi":"10.1016/j.dci.2025.105460","DOIUrl":"10.1016/j.dci.2025.105460","url":null,"abstract":"<div><div><em>Beclin1</em> is a central regulator of autophagy and cellular homeostasis, playing a critical role in the adaptation to environmental stress. Despite its importance, the expression patterns and regulatory mechanisms of <em>Beclin1</em> in bivalves remain poorly understood. In this work, we cloned the <em>Beclin1</em> gene from <em>Hyriopsis cumingii</em> (designated <em>HcBeclin1</em>) and investigated its regulatory interaction with nuclear factor erythroid 2-related factor 2 (<em>Hc</em>Nrf2). The full-length <em>HcBeclin1</em> cDNA was 1365 bp and encoded a conserved open reading frame, sharing high sequence similarity with homologous genes in other species. Reverse transcription quantitative PCR analysis revealed that <em>HcBeclin1</em> expression was significantly upregulated in the gills, hepatopancreas, and hemolymph in response to H₂O₂-induced oxidative stress. Notably, the expression levels of <em>HcBeclin1</em> in the gills and hemolymph correlated positively with those of <em>HcNrf2</em> under stress conditions. RNA interference experiments demonstrated that silencing <em>HcNrf2</em> led to a marked downregulation of <em>HcBeclin1</em>, suggesting a regulatory relationship. The promoter region of <em>HcBeclin1</em> was obtained through high-efficiency Thermal Asymmetric Interlaced PCR, revealing five putative <em>Hc</em>Nrf2 binding sites. Luciferase reporter assays identified a critical binding site within −937 to −663 bp region of the promoter, which was essential for transcriptional activation. Functional assays further confirmed that <em>Hc</em>Nrf2 regulated <em>HcBeclin1</em> expression by binding to specific cis-acting elements within its promoter. These findings offer new insights into the molecular mechanisms underlying autophagy regulation in bivalves and highlight the pivotal role of <em>HcBeclin1</em> in mediating cellular responses to oxidative stress.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"171 ","pages":"Article 105460"},"PeriodicalIF":2.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145047728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neuroanatomical profiling of the rainbow trout brain parenchyma and meninges reveals specialized immune niches and region-specific hubs for bacterial immune surveillance","authors":"Amir Mani,&nbsp;Narmin Musayeva,&nbsp;Irene Salinas","doi":"10.1016/j.dci.2025.105454","DOIUrl":"10.1016/j.dci.2025.105454","url":null,"abstract":"<div><div>Several studies have described immune responses in the teleost brain and meninges during infection, however, fundamental studies that systematically dissect how different regions of the brain maintain immune homeostasis in teleosts are missing. Here we present an in-depth investigation of the immune status of the brain parenchyma and meninges of juvenile rainbow trout (<em>Oncorhynchus mykiss</em>) at the steady state. We dissected four parenchymal brain regions including olfactory bulbs (OB), telencephalon (Tel), optic tectum (OT) and cerebellum (Cer) and its corresponding dorsal meninges. Gene expression analyses revealed higher expression of all studied immune gene markers in the meninges compared to the adjacent parenchymal areas. In the parenchyma, <em>il1b, tnfa, ighd, ighm, ight, c3ra, icam1</em>, and <em>vcam1</em> expression were highest in the OB compared to other regions. Interestingly, <em>il6</em> and <em>il10</em> expression was lowest in the OB and higher in the posterior brain. <em>Nod2a</em> and <em>nod2b</em> expression levels were highest in the OT, a finding that was confirmed <em>by in situ</em> hybridization. <em>cd45 in situ</em> hybridization revealed that most of the cd45^high (immune cells) in the brain are located at the borders of the brain parenchyma (glia limitans superficialis). The present study demonstrates the presence of regional differences in the brain immune system of rainbow trout at homeostasis and identifies previously unknown hubs poised for specialized detection of microbial products.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"171 ","pages":"Article 105454"},"PeriodicalIF":2.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145014089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effects of pseudostellaria heterophylla polysaccharide on humoral immunity and gene expression of splenic lymphocytes in chicks","authors":"BingXin Wang ,&nbsp;XiaoRan Wang ,&nbsp;XiaoXiao Li ,&nbsp;YingYue Zhu ,&nbsp;QingFu Meng ,&nbsp;ZhaoYan Zhu ,&nbsp;Yujie Guo ,&nbsp;Yadong Tian ,&nbsp;Ruirui Jiang ,&nbsp;Guirong Sun ,&nbsp;Ruili Han ,&nbsp;Xiangtao Kang ,&nbsp;ChengJun Mei ,&nbsp;FengBin Yan","doi":"10.1016/j.dci.2025.105450","DOIUrl":"10.1016/j.dci.2025.105450","url":null,"abstract":"<div><div>This study aimed to evaluate the effects of dietary Pseudostellaria heterophylla Polysaccharide (PHP) on humoral immunity and spleen lymphocyte gene expression in chicks. A total of 270 one-day-old healthy Gushi chicks were randomly allocated into three groups: Group A was fed the basic diet without vaccination; Group B received the basic diet and underwent immunization; Group C was provided with the basic diet supplemented with 200 mg/kg PHP, coupled with immunization. Samples were collected at 7, 14, 21, 28, 35, 42, and 49 days. Compared to Group A, Group B showed significantly higher specific antibody titers following the second immunization, along with increased spleen and bursa indices at day 35, and elevated serum levels of IgG, C3, and IL-4. Compared with Group B, Group C demonstrated higher specific antibody titers after the first immunization, enhanced immune organ indices at day 35, and increased serum concentrations of IgG, C3, IL-4, IL-6, and IFN-γ when compared to Group B. Additionally, gene expression analyses revealed upregulation of key immune-related genes (IFN-γ, CD40, CD80, CD86) in the spleen and (IL-4, IFN-γ, CD40, CD86) in the bursa (<em>P</em> &lt; 0.05). Transcriptome analysis of spleen lymphocytes at 35 days identified 627 differentially expressed genes between Groups B and C, enriched in pathways related to Th17 differentiation and Toll-like receptor signaling, with EGR-1 and CD40LG identified as critical genes. These findings suggest that PHP enhances humoral immunity by promoting antibody production, increasing immune organ indices, elevating serum factors, and modulating related gene expression.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"171 ","pages":"Article 105450"},"PeriodicalIF":2.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144991708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Putative orthologues of natural killer cell receptors and ligands in shrimp","authors":"Liping Zheng ,&nbsp;Omkar Vijay Byadgi ,&nbsp;Mostafa Rakhshaninejad ,&nbsp;Hans Nauwynck","doi":"10.1016/j.dci.2025.105459","DOIUrl":"10.1016/j.dci.2025.105459","url":null,"abstract":"<div><div>In vertebrates, natural killer (NK) cells play an important role in innate immunity. They detect target cells by receptor-ligand interactions and become activated or inhibited by a variety of cell surface activating and inhibitory receptors. In invertebrates, the defense against pathogens relies entirely on innate immunity, of which haemocytes are the only immune cells. In shrimp, two subpopulations of non-adherent, non-phagocytic, lymphocyte-like haemocytes were identified to have an NK-like activity. It is postulated now that they also may use the NK-like recognition mechanism by receptors and ligands. By searching for NK receptor genes in the shrimp genome database (already identified and described as such or by aligning the sequence of mammalian NK receptors with the shrimp genome), several receptors, such as tumor necrosis factor receptor superfamily (TNFRSF), semaphorins (SEMAs), sialic acid-binding immunoglobulin-like lectins (SIGLECs) and their corresponding ligands were discovered. The domain structure of these receptors and ligands were described and compared with those of mammalian domains, revealing 13 %–64 % similarity between them. The identification of shrimp NK cell surface receptors and their signaling pathways is primarily summarized in this review. Understanding the molecular interactions between NK-like receptors and their corresponding ligands in shrimp will help enhance our understanding of invertebrate immunology and controlling infectious diseases in shrimp aquaculture.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"171 ","pages":"Article 105459"},"PeriodicalIF":2.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145047727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evolutionary and molecular characterization of fish specific decoy receptor 3 isoforms (TNFRSF6B.1 and 2) in rock bream (Oplegnathus fascatus)","authors":"Sungjae Ko,&nbsp;Suhee Hong","doi":"10.1016/j.dci.2025.105453","DOIUrl":"10.1016/j.dci.2025.105453","url":null,"abstract":"<div><div>TNFRSF6B, commonly referred to as decoy receptor 3, interacts with TNFSF6, TNFSF14, and TNFSF15, thereby imparting anti-apoptotic and anti-inflammatory properties. This study identifies two isoforms, TNFRSF6B.1 and TNFRSF6B.2, in rock bream (RB) and explores their evolutionary and molecular attributes. Phylogenetic analysis indicates that the teleost isoforms TNFRSF6B.1 and TNFRSF6B.2 have diverged from ancestral forms of TNFRSF6B and TNFRSF11B. Notably, the coelacanth TNFRSF6B is positioned phylogenetically closer to frogs, while TNFRSF6B.2 branches off from TNFRSF11B. Synteny analysis reveals that the TNFRSF6B isoforms in rainbow trout are situated within the conserved mammalian TNFRSF6B locus, whereas the teleost TNFRSF6B.1 and TNFRSF6B.2 genes are located within the conserved TNFRSF11B locus. However, the amino acid sequences of TNFRSF6B.1 and TNFRSF6B.2 exhibit greater similarity to TNFRSF6B than to TNFRSF11B, with homology percentages of 33.92 % and 36.31 % for TNFRSF6B, and 27.94 % and 29.98 % for TNFRSF11B, respectively. Furthermore, protein-protein interaction analyses demonstrate associations with typical ligands of TNFRSF6B, including TNFSF14, TNFSF6, TNFSF5, and TNFSF15. Tertiary structure modeling of receptor-ligand interactions has also identified conserved TNFSF15-binding residues in both TNFRSF6B.1 and TNFRSF6B.2. In healthy rock bream, TNFRSF6B.1 expression is predominantly observed in gill and body kidney, while TNFRSF6B.2 expression is highest in fin, followed by skin and gill. <em>In vitro,</em> experiment reveals that TNFRSF6B.1 expression is significantly upregulated by calcium ionophores at 4 and 8 h, as well as by LPS at 8 or 12 h. Conversely, TNFRSF6B.2 exhibits a more pronounced response to concanavalin A and phytohemagglutinin at 4 or 12 h compared to TNFRSF6B.1. Both isoforms are upregulated in response to recombinant RB-TNFSF15 protein (RB-rTNFSF15). <em>In vivo</em>, expression levels of these genes are not significantly altered by RB-rTNFSF15 in head kidney, spleen, and blood, but are significantly upregulated in liver. In gill, RB-TNFRSF6B.1 expression is upregulated, whereas RB-TNFRSF6B.2 expression is downregulated. These findings suggest that TNFRSF6B.1 and TNFRSF6B.2 play roles in inflammatory responses and mucosal immunity, particularly in relation to TNFSF15, akin to mechanisms observed in humans.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"171 ","pages":"Article 105453"},"PeriodicalIF":2.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144999930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"VPAHPND pathogenesis and its mediated immune response in shrimp","authors":"Hourong Liu ,&nbsp;Junjin Lin ,&nbsp;Da Guo ,&nbsp;Lijie Jiang ,&nbsp;Xincang Li","doi":"10.1016/j.dci.2025.105429","DOIUrl":"10.1016/j.dci.2025.105429","url":null,"abstract":"<div><div>Shrimp aquaculture is vital for global seafood production but is severely threatened by diseases like Acute Hepatopancreatic Necrosis Disease (AHPND). Understanding AHPND-mediated immunity in shrimp requires an integrated approach drawing from various research contexts.This work described here include 1) the pathogenicity of VP_AHPND, PirA^<em>vp</em>, PirB^<em>vp</em> and PirAB^<em>vp</em> toxins, 2) the immune responses of shrimp's main immune organs, the role of miRNAs in regulating immune-related genes against VP_AHPND, heat shock proteins and small GTPases in combating VP_AHPND, 3) current knowledge on shrimp response toward PirA^<em>vp</em>, PirB^<em>vp</em>, and PirAB^<em>vp</em> Toxins, 4) knowledge of the virulence of different Vibrio spps., 5) the differential responses of various crustacean species to <em>V. parahaemolyticus</em> induced AHPND. Despite advances, gaps remain in understanding the molecular pathways and immune evasion strategies of AHPND pathogens. We provide an overview of functional reports on AHPND induced immune response benefits fundamental and applied studies.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"170 ","pages":"Article 105429"},"PeriodicalIF":2.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144687360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}