Developmental and comparative immunology最新文献_第10页

Immune response and inhibitory effects of quercetin on tilapia parvovirus: an in vitro approach 槲皮素对罗非鱼细小病毒的体外免疫应答和抑制作用。

IF 2.4 3区农林科学

Developmental and comparative immunology Pub Date : 2025-11-01 Epub Date: 2025-10-27 DOI: 10.1016/j.dci.2025.105505

Gani Taju, Allahbagash Badhusha, Seepoo Abdul Majeed, Venkatesan Rajkumar, Mohamed Jaffer Abdul Wazith, Sivaraj Mithra, Kumarasamy Kanimozhi, Azeez Sait Sahul Hameed

{"title":"Immune response and inhibitory effects of quercetin on tilapia parvovirus: an in vitro approach","authors":"Gani Taju, Allahbagash Badhusha, Seepoo Abdul Majeed, Venkatesan Rajkumar, Mohamed Jaffer Abdul Wazith, Sivaraj Mithra, Kumarasamy Kanimozhi, Azeez Sait Sahul Hameed","doi":"10.1016/j.dci.2025.105505","DOIUrl":"10.1016/j.dci.2025.105505","url":null,"abstract":"<div><div>Significant economic losses in global tilapia farming have been attributed to tilapia parvovirus (TiPV). There is an urgent need for therapeutic drug discovery, as no effective drugs currently exist to control TiPV infection. Quercetin, a polyphenolic flavonoid found in natural products, effectively inhibits the growth of several viral pathogens. This study evaluated the ability of quercetin to inhibit TiPV replication in a tilapia heart (TH) cell line. MTT assay was used to assess the cytotoxic effects of quercetin on TH cells. H<sub>2</sub>DCF-DA was used to measure quercetin-induced ROS production in TH cells. Fluorescence microscopy revealed no mitochondrial membrane and nucleus alterations at high quercetin concentrations, as assessed by rhodamine 123 and Hoechst 33,258 staining. Immune-responsive genes (<em>TNF-α, TLR-7, IL-8, MHC-II, IFNγ</em>, and <em>NF-κβ</em>) were studied in TH cells exposed to quercetin test groups (TG1-TG6) at different intervals by RT-qPCR. At 96 h post-exposure, immune-related genes were significantly upregulated in TH cells exposed to 40 and 50 μg/mL quercetin. Quercetin's inhibitory activity against TiPV infection was assessed by morphological changes, cytopathic effects, viral titre quantification by TCID<sub>50</sub>, TiPV detection by PCR, and viral load quantification by qPCR. The results showed a significant reduction in CPE, TCID<sub>50</sub>, PCR bands, and viral copies in TH cells treated with higher quercetin concentrations than in non-quercetin-exposed cells. The increase in immune gene expression and decrease in CPE, TCID<sub>50</sub>, PCR band intensity, and viral load were concentration- and time-dependent manner. This study confirmed the potent antiviral effects of quercetin against TiPV infection, suggesting its potential use as a control drug.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"172 ","pages":"Article 105505"},"PeriodicalIF":2.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145387934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clip-domain serine proteinases and their homologs: Role in crustacean immunity 剪切结构域丝氨酸蛋白酶及其同源物：在甲壳类免疫中的作用。

IF 2.4 3区农林科学

Developmental and comparative immunology Pub Date : 2025-11-01 Epub Date: 2025-09-16 DOI: 10.1016/j.dci.2025.105465

Walaiporn Charoensapsri , Miti Jearaphunt , Chanprapa Imjongjirak , Piti Amparyup

{"title":"Clip-domain serine proteinases and their homologs: Role in crustacean immunity","authors":"Walaiporn Charoensapsri , Miti Jearaphunt , Chanprapa Imjongjirak , Piti Amparyup","doi":"10.1016/j.dci.2025.105465","DOIUrl":"10.1016/j.dci.2025.105465","url":null,"abstract":"<div><div>Clip-domain serine proteinases (clip-SPs) and their non-catalytic serine proteinase homologs (SPHs) constitute a highly conserved protein family that plays a critical role in the innate immunity of crustaceans. These extracellular proteolytic enzymes contain one or more N-terminal clip domains and a C-terminal serine proteinase or proteinase-like domain. Their classification as either catalytically active clip-SPs or inactive SPHs is based on the presence or absence of a conserved catalytic triad (His-Asp-Ser). This review provides a comprehensive overview of their structural features, molecular classification, and occurrence across diverse crustacean species. Clip-SPs and SPHs act as key modulators of the innate immune system. They mediate proteolytic activation of the prophenoloxidase (proPO) cascade, which drives the melanization response, and coordinate antimicrobial and antiviral defenses to limit pathogen proliferation and promote immune clearance. They also influence the transcription of antimicrobial peptides (AMPs), likely via molecular cross-talk between the proPO cascade and Toll signaling pathway. This review summarizes current knowledge of clip-SPs and SPHs in crustaceans, emphasizing their integrative function and potential relevance to host defense mechanisms.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"172 ","pages":"Article 105465"},"PeriodicalIF":2.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145085245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in the use of dietary β-glucan in aquaculture: Structural insights, immunological benefits, and feed applications 饲料中β-葡聚糖在水产养殖中的应用进展：结构见解、免疫效益和饲料应用。

IF 2.4 3区农林科学

Developmental and comparative immunology Pub Date : 2025-11-01 Epub Date: 2025-09-21 DOI: 10.1016/j.dci.2025.105476

Yue Kong , Saikun Pan , Shengjun Wu

{"title":"Advances in the use of dietary β-glucan in aquaculture: Structural insights, immunological benefits, and feed applications","authors":"Yue Kong , Saikun Pan , Shengjun Wu","doi":"10.1016/j.dci.2025.105476","DOIUrl":"10.1016/j.dci.2025.105476","url":null,"abstract":"<div><div>β-glucan, a bioactive polysaccharide derived from fungi, yeast, grains, and algae, has become a versatile element in aquaculture feeding. This review thoroughly examines the structural differences, extraction techniques, and purification methods of β-glucans, correlating these characteristics with their biological effectiveness. Particular attention is given to their antioxidant, immunomodulatory, antibacterial, and hypolipidemic properties, along with their integration into functional diets to enhance aquatic animal performance. β-glucan supplementation has demonstrated effectiveness in enhancing development, augmenting disease resistance, and fortifying stress tolerance in many aquatic species by altering innate immunity, oxidative equilibrium, and metabolic functions. The fundamental mechanisms, encompassing receptor-mediated signaling pathways and dose-dependent responses, are meticulously examined. Furthermore, issues pertaining to delivery techniques, structural integrity, and practical use are examined. This investigation underscores β-glucan as a viable and sustainable substitute for antibiotics in enhancing aquaculture health and productivity.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"172 ","pages":"Article 105476"},"PeriodicalIF":2.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145129899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ring Finger protein 34 negatively regulates MyD88-mediated NF-κB signaling via the ubiquitin-proteasome pathway in miiuy croaker (Miichthys miiuy) 环指蛋白34通过泛素-蛋白酶体途径负调控myd88介导的NF-κB信号通路。

IF 2.4 3区农林科学

Developmental and comparative immunology Pub Date : 2025-11-01 Epub Date: 2025-09-23 DOI: 10.1016/j.dci.2025.105477

Wenjing Dong , Tianjun Xu , Yuena Sun

{"title":"Ring Finger protein 34 negatively regulates MyD88-mediated NF-κB signaling via the ubiquitin-proteasome pathway in miiuy croaker (Miichthys miiuy)","authors":"Wenjing Dong , Tianjun Xu , Yuena Sun","doi":"10.1016/j.dci.2025.105477","DOIUrl":"10.1016/j.dci.2025.105477","url":null,"abstract":"<div><div>Innate immunity constitutes a fundamental defense mechanism in host immunity, wherein myeloid differentiation factor 88 (MyD88) functions as the central adaptor protein in Toll-like receptor (TLR) signaling pathways, orchestrating teleost innate immune responses the nuclear factor-kappa B (NF-κB) pathway. To elucidate the regulatory mechanism of E3 ligase RNF34 (Ring Finger Protein 34) in this signaling cascade, we employed miiuy croaker (<em>Miichthys miiuy</em>) as a model organism and conducted a series of experiments. Luciferase reporter assays demonstrated that RNF34 exerted dose- and time-dependent inhibition on the MyD88-mediated NF-κB signaling pathway. This inhibitory effect persisted under LPS stimulation, confirming RNF34's stable regulatory function. Western blot analysis further revealed that RNF34 negatively regulated MyD88 protein expression, and this regulatory effect was significantly enhanced under LPS stimulation. Mechanistic investigations showed that cycloheximide (CHX) chase assays indicated RNF34 significantly shortened MyD88 protein half-life; treatment with the proteasome inhibitor MG132 completely reversed RNF34-mediated MyD88 degradation; and ubiquitination assays demonstrated that RNF34 substantially enhanced MyD88 ubiquitination levels. These findings collectively indicate that RNF34 promotes MyD88 ubiquitination, leading to its proteasomal degradation and inhibition of NF-κB signaling pathway activation. This study enriches the understanding of RNF34 as a negative immune regulator in miiuy croaker, providing evidence for the regulatory mechanism of the NF-κB signaling pathway in teleosts and offering insights into the precise maintenance of immune homeostasis in teleost fishes.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"172 ","pages":"Article 105477"},"PeriodicalIF":2.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145148262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Wnt/β-catenin signalling pathway regulates ovarian development in the swimming crab Portunus trituberculatus Wnt/β-catenin信号通路调控三瘤梭子蟹卵巢发育。

IF 2.4 3区农林科学

Developmental and comparative immunology Pub Date : 2025-11-01 Epub Date: 2025-10-15 DOI: 10.1016/j.dci.2025.105492

Xiaona Zhang , Huan Wang , Lei Liu , Ronghua Li , Yangfang Ye , Weiwei Song , Ce Shi , Chunlin Wang , Changkao Mu

{"title":"The Wnt/β-catenin signalling pathway regulates ovarian development in the swimming crab Portunus trituberculatus","authors":"Xiaona Zhang , Huan Wang , Lei Liu , Ronghua Li , Yangfang Ye , Weiwei Song , Ce Shi , Chunlin Wang , Changkao Mu","doi":"10.1016/j.dci.2025.105492","DOIUrl":"10.1016/j.dci.2025.105492","url":null,"abstract":"<div><div>Wnt/β-catenin signalling regulates many physiological processes in organisms, including development and tissue homeostasis maintenance. Signal transduction in this pathway involves the regulation of its multiprotein complex, which targets β-catenin for proteasomal degradation. The genes encoding the cytoplasmic regulatory proteins β-catenin and GSK-3β in <em>Portunus trituberculatus</em> were successfully cloned and characterized here. <em>Ptβ-catenin</em> was most highly expressed in hemocytes, <em>PtGSK-3β</em> in ovarian tissue, and both genes were significantly upregulated at ovarian stage III, as revealed by quantitative real-time RT-PCR analysis. Continuous injection of the Wnt/β-catenin signalling activator CHIR-98014 resulted in decreased expression of <em>PtGSK-3β</em>, increased expression of <em>Ptβ-catenin</em>, decreased expression of <em>PtVg</em> (vitellogenin) and the content of r<em>PtVn</em> (vitellin). Additionally, it resulted in a significant reduction in both gonadosomatic index (GSI) and oocyte diameter. HE staining revealed diminished vitellogenic granules and the formation of cytoplasmic vacuoles in the oocytes. By contrast, continuous injection ETC-159, a potent inhibitor of Wnt/β-catenin signalling caused increased expression of <em>PtGSK-3β</em>, decreased expression of <em>Ptβ-catenin</em>, increased expression of <em>PtVg</em> and the content of r<em>PtVn</em>. Furthermore, it resulted in an increased GSI, larger oocyte diameter, and HE staining revealed a greater number of yolk granules and a tight arrangement of oocytes. This study elucidates the critical regulatory function of Wnt/β-catenin signaling in <em>P. trituberculatus</em> ovarian development, establishing a foundation for future investigations into the molecular mechanisms governing crustacean reproductive physiology.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"172 ","pages":"Article 105492"},"PeriodicalIF":2.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145307041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Peripheral blood mononuclear cell-driven cytokine and transcription factors induction confers resistance to Haemonchus contortus in Black Bengal goats 黑孟加拉山羊外周血单核细胞驱动细胞因子和转录因子诱导对扭曲血蜱的抗性。

IF 2.4 3区农林科学

Developmental and comparative immunology Pub Date : 2025-11-01 Epub Date: 2025-09-19 DOI: 10.1016/j.dci.2025.105470

Nurnabi Ahmed , Babul Chandra Roy , Amitav Biswas , Md Rajiur Rahaman Rabbi , Md Mahfuzur Rahman Sajib , Mohammad Manjurul Hasan , Hiranmoy Biswas , MD Hasanuzzaman Talukder

{"title":"Peripheral blood mononuclear cell-driven cytokine and transcription factors induction confers resistance to Haemonchus contortus in Black Bengal goats","authors":"Nurnabi Ahmed , Babul Chandra Roy , Amitav Biswas , Md Rajiur Rahaman Rabbi , Md Mahfuzur Rahman Sajib , Mohammad Manjurul Hasan , Hiranmoy Biswas , MD Hasanuzzaman Talukder","doi":"10.1016/j.dci.2025.105470","DOIUrl":"10.1016/j.dci.2025.105470","url":null,"abstract":"<div><div><em>Haemonchus contortus</em> is a major threat to small ruminant health and productivity. Although early Th2 cytokine and transcription factor expression confers protection in naturally resistant breeds, the immune basis of resistance in Black Bengal goats (BBG) remains unexplored. We compare early PBMC-mediated cytokine and transcriptional responses and their direct effects on larval motility between naive and primed BBG kids during the first seven days of infection. Kids were primed with 2000 L<sub>3</sub> weekly for four weeks or left naive, then challenged with 10,000 L<sub>3</sub>. Two kids per group were sacrificed on each time point. Whole blood was collected pre-mortem for differential counts and PBMC isolation, while abomasal mucosa and draining LN were harvested for histology and RNA extraction. Primed kids exhibited a 58 % reduction in abomasal L<sub>4</sub> burden by day 7, elevated PCV (<em>p <</em> 0.05), and a threefold greater increase in LN weight compared to naive kids. Histopathology revealed significantly enhanced eosinophil and neutrophil infiltration in abomasal mucosa of primed kids. Cytokine and gene expression assay showed early upregulation of Interleukin (IL)-4, IL-5, IL-13, IL-33, MCP-1, CXCL-1, TLR-2, and GAL-14 (p < 0.05). <em>In vitro</em>, co-culture with primed PBMCs reduced L<sub>3</sub> motility compared to naive PBMCs (p < 0.01) and L<sub>3</sub> pretreated with primed PBMCs resulted in a 60 % reduction in fecal egg counts by week 5 (p < 0.001). This is the first study to integrate daily PBMC transcriptomics with functional motility and infectivity assays in BBGs. The findings identify novel biomarkers, inform selective breeding and immunoprophylactic strategies for sustainable parasite control.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"172 ","pages":"Article 105470"},"PeriodicalIF":2.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145102552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A flow cytometric approach to identifying the relative abundance and functional capacities of hemocyte subsets in the American cockroach, Periplaneta americana (L.) 流式细胞术方法鉴定美洲大蠊血细胞亚群的相对丰度和功能能力。

IF 2.4 3区农林科学

Developmental and comparative immunology Pub Date : 2025-11-01 Epub Date: 2025-11-04 DOI: 10.1016/j.dci.2025.105508

Faith J. Boyer-Millander , Aaron T. Martin , Chadwick A. Hamm , Arthur G. Appel , Elizabeth Hiltbold Schwartz

{"title":"A flow cytometric approach to identifying the relative abundance and functional capacities of hemocyte subsets in the American cockroach, Periplaneta americana (L.)","authors":"Faith J. Boyer-Millander , Aaron T. Martin , Chadwick A. Hamm , Arthur G. Appel , Elizabeth Hiltbold Schwartz","doi":"10.1016/j.dci.2025.105508","DOIUrl":"10.1016/j.dci.2025.105508","url":null,"abstract":"<div><div>There is growing interest in insects as subjects for comparative immunological studies; however, very little has been done to quantitatively characterize insect immune cells using modern techniques such as flow cytometry, and virtually no work of this kind has been done in <em>Periplaneta americana</em>. Here, we use an array of general molecular probes including fluorescent lectins, lysosomal indicators, and functional assays to distinguish and characterize insect immune cells (hemocytes) based on cell markers and functions. We have utilized fluorescent tracers of lysosomal content, ROS production, and phagocytosis, as well as microscopic examination of morphology and melanization to distinguish hemocyte types based on these functions. Our findings support the use of lectins as an additional means of separating at least three populations of cockroach immune cells coupled with morphological measurements such as size and complexity. Our results indicate that many functions are enriched in the more granular hemocyte population as these are the cells that exhibit phagocytosis and melanization.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"172 ","pages":"Article 105508"},"PeriodicalIF":2.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145451150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression and characterization of activation-induced deaminase (AID) with dehydroaminase activity and its localization in IgM+ lymphocytes of spleen of common carp (Cyprinus carpio) 鲤脾脏IgM+淋巴细胞中具有脱氢氨酶活性的激活诱导脱氨酶（AID）的表达、表征及其定位

IF 2.4 3区农林科学

Developmental and comparative immunology Pub Date : 2025-11-01 Epub Date: 2025-10-10 DOI: 10.1016/j.dci.2025.105489

Guangcai Wei , Jianhui Zhao , Qingyun Zuo , Manting Sun , Hua Wei , Haofeng Wang , Guiwen Yang , Eakapol Wangkahart , Lei Wang , Fumiao Zhang

{"title":"Expression and characterization of activation-induced deaminase (AID) with dehydroaminase activity and its localization in IgM+ lymphocytes of spleen of common carp (Cyprinus carpio)","authors":"Guangcai Wei , Jianhui Zhao , Qingyun Zuo , Manting Sun , Hua Wei , Haofeng Wang , Guiwen Yang , Eakapol Wangkahart , Lei Wang , Fumiao Zhang","doi":"10.1016/j.dci.2025.105489","DOIUrl":"10.1016/j.dci.2025.105489","url":null,"abstract":"<div><div>Activation-induced deaminase (AID) is a key enzyme that catalyzes the deamination of cytosine to uracil in DNA, driving somatic hypermutation (SHM) and class switch recombination (CSR) of antibody genes to promote antibody diversification in the adaptive immunity. The AID gene from <em>Cyprinus carpio</em> (CcAID) has a full-length open reading frame of 633 bp and encodes a protein of 210 amino acids. CcAID shares structural and functional similarities with apolipoprotein B mRNA-editing enzyme, catalytic polypeptide-like (APOBEC) family members and contains a conserved catalytic domain characteristic of cytidine deaminases. Notably, the absence of two key phosphorylation sites in CcAID (Ser38 and Tyr184) may be a significant factor contributing to the markedly lower levels of somatic hypermutation (SHM) in teleost fish. A high level of CcAID transcript expression was detected in carp larvae on the sixth day after fertilization. Intraperitoneal injection of T-independent and T-dependent antigens preferentially upregulated CcAID expression in head kidney and splenic lymphocytes. Immunofluorescence analysis confirmed its expression in IgM<sup>+</sup> spleen lymphocytes. Recombinant CcAID exhibited cytidine deaminase activity. These findings indicate that CcAID is specifically expressed in IgM<sup>+</sup> spleen lymphocytes, suggesting its potential role in antibody production.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"172 ","pages":"Article 105489"},"PeriodicalIF":2.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145279227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Elucidating the regulatory role of Nrf2 in Beclin1-mediated autophagy in freshwater bivalve Hyriopsis cumingii 阐明Nrf2在淡水双壳类三角帆蚌beclin1介导的自噬中的调控作用

IF 2.4 3区农林科学

Developmental and comparative immunology Pub Date : 2025-10-01 Epub Date: 2025-09-11 DOI: 10.1016/j.dci.2025.105460

Yuzhuo He, Shaoyu Hu, Qinglin Yang, Xiaobo Yu, Yanhong Li, Zhengli Wu

{"title":"Elucidating the regulatory role of Nrf2 in Beclin1-mediated autophagy in freshwater bivalve Hyriopsis cumingii","authors":"Yuzhuo He, Shaoyu Hu, Qinglin Yang, Xiaobo Yu, Yanhong Li, Zhengli Wu","doi":"10.1016/j.dci.2025.105460","DOIUrl":"10.1016/j.dci.2025.105460","url":null,"abstract":"<div><div><em>Beclin1</em> is a central regulator of autophagy and cellular homeostasis, playing a critical role in the adaptation to environmental stress. Despite its importance, the expression patterns and regulatory mechanisms of <em>Beclin1</em> in bivalves remain poorly understood. In this work, we cloned the <em>Beclin1</em> gene from <em>Hyriopsis cumingii</em> (designated <em>HcBeclin1</em>) and investigated its regulatory interaction with nuclear factor erythroid 2-related factor 2 (<em>Hc</em>Nrf2). The full-length <em>HcBeclin1</em> cDNA was 1365 bp and encoded a conserved open reading frame, sharing high sequence similarity with homologous genes in other species. Reverse transcription quantitative PCR analysis revealed that <em>HcBeclin1</em> expression was significantly upregulated in the gills, hepatopancreas, and hemolymph in response to H<sub>2</sub>O<sub>2</sub>-induced oxidative stress. Notably, the expression levels of <em>HcBeclin1</em> in the gills and hemolymph correlated positively with those of <em>HcNrf2</em> under stress conditions. RNA interference experiments demonstrated that silencing <em>HcNrf2</em> led to a marked downregulation of <em>HcBeclin1</em>, suggesting a regulatory relationship. The promoter region of <em>HcBeclin1</em> was obtained through high-efficiency Thermal Asymmetric Interlaced PCR, revealing five putative <em>Hc</em>Nrf2 binding sites. Luciferase reporter assays identified a critical binding site within −937 to −663 bp region of the promoter, which was essential for transcriptional activation. Functional assays further confirmed that <em>Hc</em>Nrf2 regulated <em>HcBeclin1</em> expression by binding to specific cis-acting elements within its promoter. These findings offer new insights into the molecular mechanisms underlying autophagy regulation in bivalves and highlight the pivotal role of <em>HcBeclin1</em> in mediating cellular responses to oxidative stress.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"171 ","pages":"Article 105460"},"PeriodicalIF":2.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145047728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neuroanatomical profiling of the rainbow trout brain parenchyma and meninges reveals specialized immune niches and region-specific hubs for bacterial immune surveillance 虹鳟鱼脑实质和脑膜的神经解剖学分析揭示了专门的免疫龛和区域特异性中心的细菌免疫监测。

IF 2.4 3区农林科学

Developmental and comparative immunology Pub Date : 2025-10-01 Epub Date: 2025-09-05 DOI: 10.1016/j.dci.2025.105454

Amir Mani, Narmin Musayeva, Irene Salinas

{"title":"Neuroanatomical profiling of the rainbow trout brain parenchyma and meninges reveals specialized immune niches and region-specific hubs for bacterial immune surveillance","authors":"Amir Mani, Narmin Musayeva, Irene Salinas","doi":"10.1016/j.dci.2025.105454","DOIUrl":"10.1016/j.dci.2025.105454","url":null,"abstract":"<div><div>Several studies have described immune responses in the teleost brain and meninges during infection, however, fundamental studies that systematically dissect how different regions of the brain maintain immune homeostasis in teleosts are missing. Here we present an in-depth investigation of the immune status of the brain parenchyma and meninges of juvenile rainbow trout (<em>Oncorhynchus mykiss</em>) at the steady state. We dissected four parenchymal brain regions including olfactory bulbs (OB), telencephalon (Tel), optic tectum (OT) and cerebellum (Cer) and its corresponding dorsal meninges. Gene expression analyses revealed higher expression of all studied immune gene markers in the meninges compared to the adjacent parenchymal areas. In the parenchyma, <em>il1b, tnfa, ighd, ighm, ight, c3ra, icam1</em>, and <em>vcam1</em> expression were highest in the OB compared to other regions. Interestingly, <em>il6</em> and <em>il10</em> expression was lowest in the OB and higher in the posterior brain. <em>Nod2a</em> and <em>nod2b</em> expression levels were highest in the OT, a finding that was confirmed <em>by in situ</em> hybridization. <em>cd45 in situ</em> hybridization revealed that most of the cd45<sup>high</sup> (immune cells) in the brain are located at the borders of the brain parenchyma (glia limitans superficialis). The present study demonstrates the presence of regional differences in the brain immune system of rainbow trout at homeostasis and identifies previously unknown hubs poised for specialized detection of microbial products.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"171 ","pages":"Article 105454"},"PeriodicalIF":2.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145014089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0