Developmental and comparative immunology最新文献

{"title":"Interactions between microbiota and immunity shape pathogen acquisition and fitness in Amblyomma spp. ticks","authors":"Solange C. Antão ,&nbsp;Daniel B. Pavanelo ,&nbsp;Eliane Esteves ,&nbsp;Marcelly B. Nassar ,&nbsp;Beatriz I. Alonso ,&nbsp;Pablo Vera ,&nbsp;Marcelo B. Labruna ,&nbsp;Petr Kopáček ,&nbsp;Sirlei Daffre ,&nbsp;Ludek Zurek ,&nbsp;Fernanda Dias da Silva ,&nbsp;Marisa Farber ,&nbsp;Andréa C. Fogaça","doi":"10.1016/j.dci.2025.105493","DOIUrl":"10.1016/j.dci.2025.105493","url":null,"abstract":"<div><div>Besides carrying pathogens, ticks also harbor commensal and mutualistic microorganisms that constitute their microbiota. This microbial community can modulate the tick immune system and influence pathogen acquisition, either facilitating or hindering colonization. Additionally, the microbiota may impact tick fitness. Although the ticks <em>Amblyomma sculptum</em> and <em>Amblyomma aureolatum</em> are important vectors of <em>Rickettsia rickettsii</em>, the causative agent of Brazilian spotted fever, <em>A. sculptum</em> is much less susceptible to infection than <em>A. aureolatum</em>. Intriguingly, while <em>A. aureolatum</em> midgut harbors an abundant microbiota, mostly composed of bacteria of the <em>Francisella</em> genus, <em>A. sculptum</em> presents a markedly reduced bacterial community. In the current study, we quantified the total bacterial load also in the salivary glands and ovaries of adult <em>A. sculptum</em> and <em>A. aureolatum</em>, besides midgut. Across all analyzed organs, bacterial loads were consistently lower in <em>A. sculptum</em> than in <em>A. aureolatum</em>, regardless of whether the ticks had fed on naïve or <em>R. rickettsii</em>-inoculated hosts. High-throughput sequencing of the V3-V4 hypervariable region of the bacterial 16S rRNA gene revealed that <em>Francisella</em> endosymbiont is the dominant taxon in all organs of control <em>A. aureolatum</em>, with the highest relative frequency in the ovaries and the lowest in the midgut. The highest relative frequency of <em>Francisella</em> in the ovaries correlates with the lower susceptibility of this organ to <em>R. rickettsii</em>, suggesting that the endosymbiosis may limit infection. No 16S rRNA gene sequences could be obtained for <em>A. sculptum</em> samples, likely due to their low bacterial content. To investigate the role played by the microbiota on rickettsial acquisition and tick fitness, <em>A. aureolatum</em> engorged females were treated with either tetracycline or ciprofloxacin. Tetracycline treatment significantly reduced bacterial loads and antimicrobial peptide transcript levels in the eggs, and this was followed by a higher acquisition of <em>R. rickettsii</em> by hatched larvae. Additionally, tetracycline negatively impacted tick development, reducing the molt success from the larval to the nymphal stage. These results suggest that maternal microbiota plays a role in shaping offspring immunity, pathogen susceptibility, and tick development. The multifaceted role of tick microbiota in both development and vector competence underscores its potential as a biotechnological resource for developing new strategies to control tick-borne diseases.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"172 ","pages":"Article 105493"},"PeriodicalIF":2.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145299173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of Integrin β1 and its effects on cell adhesion, migration and apoptosis in Sebastes schlegelii","authors":"Xiaohua Yuan ,&nbsp;Xinqing Ma ,&nbsp;Haitao Sun ,&nbsp;Min Zhang ,&nbsp;Jingjing Wang","doi":"10.1016/j.dci.2025.105467","DOIUrl":"10.1016/j.dci.2025.105467","url":null,"abstract":"<div><div>Integrin β1, a transmembrane receptor, plays a pivotal role in regulating cell adhesion, migration, signal transduction, and apoptosis in diverse tissues and cell types. In this study, we identified <em>integrin β1</em> from <em>Sebastes schlegelii</em> (<em>SsITGβ1</em>). The <em>SsITGβ1</em> cDNA spans 4017 bp, including a 332 bp 5′ untranslated region (UTR), a 1285 bp 3′ UTR, and a 2400 bp open reading frame (ORF). The cytoplasmic tail contains two conserved motifs, NPIY and NPKY. Its protein comprises a signal peptide, an INB domain, an EGF-2 domain, an integrin-B-tail domain, a transmembrane domain, and an integrin-β-cyt domain. Phylogenetic and homology analyses revealed that integrin β1 is highly conserved among vertebrates. Tissue-specific expression analysis revealed that <em>SsITGβ1</em> mRNA levels were highest in liver tissue. In the stimulation experiment of <em>Edwardsiella piscicida</em>, the expression of <em>SsITGβ1</em> in spleen tissues changed significantly. Subcellular localization confirmed that <em>SsITGβ1</em> is located in the cell membrane. Furthermore, in the <em>S. schlegelii</em> intestinal cell line, assays for cell-matrix adhesion, wound healing, and flow cytometry demonstrated that the overexpression of <em>SsITGβ1</em> (via the plasmid pcDNA3.1-<em>SsITGβ1</em>) enhanced cell adhesion, promoted migration, and reduced apoptosis. In contrast, knockdown of <em>SsITGβ1</em> using small interfering RNA resulted in the opposite effects. Analysis of mRNA changes in <em>SsITGβ1</em>-regulated genes indicated that <em>SsITGβ1</em> may influence the aforementioned cellular activities via the <em>FAK</em>/<em>ILK</em>/<em>RhoA</em> and <em>Bcl-2</em>/<em>IL-1β</em>/<em>TGF-β1</em> signaling pathways. This study offers a comprehensive characterization and functional analysis of integrin β1 in <em>S. schlegelii</em>, establishing a foundation for investigating its role in the regulatory mechanisms of immune responses and cellular activities.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"172 ","pages":"Article 105467"},"PeriodicalIF":2.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145091349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation and characterization of monoclonal antibodies against porcine caspase-7","authors":"Chenyi Guo ,&nbsp;Lanjie Yu ,&nbsp;Shuangshuang Zhang ,&nbsp;Yongsheng Cao ,&nbsp;Wenlong Zhang","doi":"10.1016/j.dci.2025.105485","DOIUrl":"10.1016/j.dci.2025.105485","url":null,"abstract":"<div><div>Cysteine-aspartic proteases (Caspases) play a central role in programmed cell death (PCD). Caspase-7 is an effector caspase involved in the execution phase of apoptosis and pyroptosis. Determining the expression and activation of caspase-7 is often an essential experiment in studies on PCD. Currently, commercially available antibodies specific to human and mouse caspase-7 have facilitated research on PCD in these species. A specific antibody against porcine caspase-7 (pcaspase-7) is needed, as PCD has been closely linked to porcine diseases. In this study, a recombinant pcaspase-7 protein (rpcaspase-7) with cysteine replaced by alanine at the 186 position was prokaryotically expressed and purified. This recombinant protein was used to immunize BALB/c mice to generate pcaspase-7-specific monoclonal antibodies (mAbs). The prepared 7-4E1-F4-D2-E1 mAb targets the 90–100 amino acid (aa) oligopeptide (TDKDAEALFKC) of pcaspase-7, while the mAbs 7-3F1-E2-H2-D6, 7-3F1-E2-H2-D1, 7-4E5-C11-C9-B5, and 7-4E5-C11-C9-F11 target the 45–55 aa oligopeptide (GSSVKIPRDRE). All prepared mAbs can discriminate pcaspase-7 from pcaspase-1, -3, -8, and -13. The mAbs targeting the 45–55 aa oligopeptide can also distinguish pcaspase-7 from human, hamster, and bovine caspase-7. The 7-4E1-F4-D2-E1 mAb recognizes pcaspase-7 in Western blot assays but not in immunofluorescence assays. These mAbs can be used to purify prokaryotically expressed pcaspase-7 via immunoprecipitation and to detect pcaspase-7 activation in virus-infected porcine cells. Thus, they represent valuable tools for future structural and functional studies of pcaspase-7. Additionally, this study demonstrated that pcaspase-7 can cleave porcine GSDMD, revealing a previously unrecognized function of pcaspase-7.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"172 ","pages":"Article 105485"},"PeriodicalIF":2.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145237860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated analysis of microRNA expression profiles in Takifugu obscurus kidney following Vibrio harveyi challenge","authors":"Rui Shen,&nbsp;Hao Wu,&nbsp;Qian-Hui Yuan,&nbsp;Zhe Zhao,&nbsp;Ying Huang","doi":"10.1016/j.dci.2025.105478","DOIUrl":"10.1016/j.dci.2025.105478","url":null,"abstract":"<div><div>MicroRNAs (miRNAs), a class of small non-coding RNAs critical for post-transcriptional gene regulation, serve as key immune modulators in vertebrates. However, their roles in teleost disease resistance remain underexplored. In the present study, a comprehensive miRNA transcriptome analysis was conducted in <em>Takifugu obscurus</em> under <em>Vibrio harveyi</em> challenge using high-throughput RNA sequencing. Three miRNA libraries (ToCG, ToEG1, and ToEG2) were constructed from kidney tissues of <em>T</em>. <em>obscurus</em> collected at 0 h (non-infected control), 6 h, and 24 h post-infection. Bioinformatics analysis identified 1093 miRNAs, including 195 novel miRNAs, and revealed 175 differentially expressed miRNAs (DEmiRNAs) across infection timepoints. Validation experiments via quantitative real-time PCR confirmed the expression patterns of 16 randomly selected DEmiRNAs, demonstrating high consistency with sequencing data. Functional enrichment analysis of DEmiRNAs highlighted significant associations with immune regulatory pathways, including mitogen-activated protein kinase signaling, Forkhead box O signaling, erythroblastic leukemia viral oncogene homolog signaling, and endocytosis pathways. Notably, several DEmiRNAs exhibited temporal expression patterns, suggesting stage-specific immunoregulatory functions during bacterial pathogenesis. This study suggests a potential miRNA regulatory network in <em>T. obscurus</em> during <em>V. harveyi</em> infection, providing crucial insights into conserved and species-specific miRNA-mediated immune mechanisms in teleosts.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"172 ","pages":"Article 105478"},"PeriodicalIF":2.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145120084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of feed supplementation with Lactobacillus reuteri and Bacillus subtilis on the liver immunity in largemouth bass (Micropterus salmoides)","authors":"Chong Wang ,&nbsp;Xiaodi Hu ,&nbsp;Huijuan Tang ,&nbsp;Wei Ge ,&nbsp;Lijun Di ,&nbsp;Jixing Zou ,&nbsp;Aiguo Zhou ,&nbsp;Zongbin Cui","doi":"10.1016/j.dci.2025.105486","DOIUrl":"10.1016/j.dci.2025.105486","url":null,"abstract":"<div><div>In the culture of largemouth bass (<em>Micropterus salmoides</em>), probiotics play an important role. Probiotics such as <em>Lactobacillus reuteri</em> and <em>Bacillus subtilis</em> can enhance the immune systems of fish like <em>M. salmoides</em> and help them prevent diseases. The largemouth bass in this experiment were fed a diet containing <em>B. subtilis</em> (BS, 10⁷ CFU/g) and <em>L. reuteri</em> (LR, 10⁷ CFU/g) for 35 days. Investigations were conducted into how the two probiotics affected the liver antioxidant capacity and liver immunity of <em>M. salmoides</em>. The findings revealed that both the BS and LR groups activated the <em>Nrf2</em>/<em>keap1</em> signaling pathway, significantly increasing the expression of genes such as <em>Nrf2</em>, <em>SOD1</em>, <em>SOD2</em>, <em>GST</em>, and <em>CAT</em>. Moreover, the BS group notably augmented the levels of AKP, T-AOC, SOD, and CAT in the liver. Importantly, the key immune-related pathways enriched for differentially expressed genes in the BS group for hepatic transcriptional machinery encompassed complement and coagulation cascades, IL-17 signaling pathway, and chemokine signaling pathway. In conclusion, <em>B. subtilis</em> and <em>L. reuteri</em> demonstrate the ability to enhance the hepatic antioxidant capacity and immunocompetence of <em>M. salmoides</em>. However, significant differences exist in the intensity of their effects and the types of immune pathways activated in the liver. Both hold promising application value, and this study lays a foundational groundwork for the utilization of <em>B. subtilis</em> and <em>L. reuteri</em> in <em>M. salmoides</em> aquaculture.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"172 ","pages":"Article 105486"},"PeriodicalIF":2.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145250292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional characterization of complement receptor 3 (CR3) in Nile tilapia (Oreochromis niloticus): Insights into CD11b/CD18-mediated immunity against bacterial infections","authors":"Yang Lei ,&nbsp;Weiheng Shi ,&nbsp;Yanxi Guo ,&nbsp;Yuqing Lin ,&nbsp;Jianmin Ye ,&nbsp;Liting Wu","doi":"10.1016/j.dci.2025.105469","DOIUrl":"10.1016/j.dci.2025.105469","url":null,"abstract":"<div><div>Complement receptor 3 (CR3), also known as integrin αMβ2, CD11b/CD18, or Mac-1, is a heterodimeric leukocyte-specific integrin composed of the αM (CD11b) and β2 (CD18) subunits. To elucidate the role of CR3 in immunity in Nile tilapia (<em>Oreochromis niloticus</em>), we cloned and characterized the αM (<em>On</em>CD11b) and β2 (<em>On</em>CD18) subunits and investigated their functions both <em>in vivo</em> and <em>in vitro</em>. Sequence analysis revealed that <em>On</em>CD11b contains a 3378-bp open reading frame (ORF) encoding a protein of 1128 amino acids (124.7 kDa), while <em>On</em>CD18 comprises a 2337-bp ORF encoding 778 amino acids (85.7 kDa). Structural alignment demonstrated high degree of conservation in the von Willebrand factor type A (vWFA) domains of both subunits, with significant homology to CD11b and CD18 orthologs across species. Phylogenetic analysis confirmed that <em>On</em>CD11b and <em>On</em>CD18 cluster within the teleost-specific CD11b and CD18 clades, respectively. Tissue-specific expression profiling indicated predominant expression of <em>On</em>CD11b in the head kidney and <em>On</em>CD18 in the spleen. Both subunits were significantly upregulated in these tissues following challenges with <em>Streptococcus agalactiae</em> (<em>S. agalactiae</em>) and <em>Aeromonas hydrophila</em> (<em>A. hydrophila</em>), suggesting their involvement in pathogen-induced immune responses. <em>In vitro</em> functional assays demonstrated that the recombinant vWFA domains of <em>On</em>CD11b and <em>On</em>CD18 exhibited specific binding capacity to <em>S. agalactiae</em>, <em>A. hydrophila</em>, and lipopolysaccharide, highlighting their role as pattern recognition receptors. Crucially, <em>in vivo</em> knockdown of <em>On</em>CD11b or <em>On</em>CD18 resulted in a significant increase in bacterial load in tilapia tissues following <em>S. agalactiae</em> infection, underscoring their essential role in host defense. These findings collectively demonstrate that <em>On</em>CD11b and <em>On</em>CD18 are pivotal components of the immune system in Nile tilapia, facilitating bacterial clearance through direct pathogen recognition pathway. This study provides new insights into the evolutionarily conserved mechanisms of CR3-mediated immunity and potential therapeutic targets for bacterial infections in aquaculture species.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"172 ","pages":"Article 105469"},"PeriodicalIF":2.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145098908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comparative analysis of the gut microbiome and immune gene expression in two Penaeus monodon populations","authors":"Pacharaporn Angthong ,&nbsp;Tanaporn Uengwetwanit ,&nbsp;Sopacha Arayamethakorn ,&nbsp;Panyisa Potibut ,&nbsp;Mongkhol Phanthura ,&nbsp;Wanilada Rungrassamee ,&nbsp;Sage Chaiyapechara","doi":"10.1016/j.dci.2025.105514","DOIUrl":"10.1016/j.dci.2025.105514","url":null,"abstract":"<div><div>Maintaining intestinal homeostasis is important for shrimp health in aquaculture, particularly under the constant exposure to environmental and biological stressors. While much research has focused on disease-related dysbiosis, the fundamental mechanisms of homeostasis under normal conditions remain largely uncharacterized. To address this gap, postlarval black tiger shrimp (<em>Penaeus monodon</em>) from two distinct genetic lineages, domestic and imported broodstock, were reared in a shared aquaculture environment for three months to observe baseline interaction between intestinal microbiota and immune responses. Intestinal microbiota was examined using 16S rRNA gene (V3-V4) sequencing, and transcriptomic profiles of the intestines were analyzed using RNA sequencing. Average final weight was not significantly different (<em>p</em> = 0.66) between two sources. Microbiota from the intestines of shrimp from different sources showed significant differences at all sampling periods throughout the feeding trial (<em>p</em> &lt; 0.05), based on PERMANOVA results. <em>Vibrio, Colwellia, Pseudoalteromonas, Shewanella, and Tenacibaculum</em> were the dominant bacterial genera in the intestines of shrimp from both groups. Several discriminant genera between the two groups were also identified, including <em>Pseudoalteromonas, Mesoflavibacter, Maribacter, Spongiimonas, Desulfobulbus</em>, and uncultured JGI o_JGI 0000069-P22 (Patescibacteria group). Transcriptomic analysis revealed that while both groups expressed immune-related genes across broad functional categories like pattern recognition proteins (PRPs), proteinase and protease inhibitors (PPIs), and antimicrobial peptides (AMPs), they appeared to employ different sets of individual genes or isoforms within these functional groups. These findings suggest that shrimp's immune systems during normal conditions can utilize different pathways within the same functional categories to control the intestinal microbiome. The pathways preference could be due to their genetic background and baseline microbiota. A better understanding of the unstressed host–microbiota interaction could help guide the microbiome management strategies to maintain gut homeostasis for sustainable disease control in shrimp aquaculture.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"172 ","pages":"Article 105514"},"PeriodicalIF":2.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145511874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening and validation of Galectin3-interacting proteins from Japanese flounder (Paralichthys olivaceus)","authors":"Lirong Jiang ,&nbsp;Baoshan Guo ,&nbsp;Yiping Lin ,&nbsp;Qingyue Xu ,&nbsp;Dong Zheng ,&nbsp;Shun Zhou ,&nbsp;Yunji Xiu","doi":"10.1016/j.dci.2025.105504","DOIUrl":"10.1016/j.dci.2025.105504","url":null,"abstract":"<div><div>Galectins are proteins that play a crucial role in the innate immune response against pathogenic microorganisms. Galectin3, the only chimera-type galectin found in vertebrates, have been demonstrated a variety of biological activities in vitro, such as activation of cells, modulation of cell adhesion and regulation of apoptosis. In addition, galectin3 has been shown to be associated with inflammatory responses. However, it is unknown exactly how galectin3 contributes to the immunological response. In this research, to find the interacting proteins of galectin3, TMT liquid chromatography-tandem mass spectrometry (TMT LC-MS/MS), co-immunoprecipitation and liquid chromatography-tandem mass spectrometry (Co-IP LC-MS/MS) technologies and Co-IP Western blot (Co-IP WB) were applied. Intestinal epithelial cells of <em>Paralichthys olivaceus</em> (PoIEC) were transfected with pcDNA3.1-Galectin3 to obtain an overexpression plasmid and validated it by Western blot. Then we searched the proteins that may interact with galectin3 by TMT LC-MS/MS and Co-IP LC-MS/MS experiments, which suggest that cathepsin B (CTSB) and heat shock cognate 71 kDa protein (HSC71) are potential interacting proteins of galectin3. Then, HSC71 was verified as an interacting protein with galectin3 by Co-IP WB. In conclusion, our findings suggest that HSC71 acts as a target protein for galectin3, helping to elucidate the mechanism of galectin3 involvement in the inflammatory response and further exploring the cellular activation of fish pathogen signaling recognition, transmission and inflammatory cascade response.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"172 ","pages":"Article 105504"},"PeriodicalIF":2.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145387910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic analysis reveals the mechanism of viral modulation of host immunity facilitating calicivirus replication in Pelteobagrus fulvidraco","authors":"Siyu Liu ,&nbsp;Linyun Lin ,&nbsp;Yue Zou ,&nbsp;Dong Liu ,&nbsp;Qiongyao Guo ,&nbsp;Xin Chu ,&nbsp;Jinyu Shen ,&nbsp;Xiaoyi Pan","doi":"10.1016/j.dci.2025.105503","DOIUrl":"10.1016/j.dci.2025.105503","url":null,"abstract":"<div><div>Yellow catfish (<em>Pelteobagrus fulvidraco</em>), an economically significant aquaculture species in East Asia, faces substantial threats from pathogens, with yellow catfish calicivirus (YcCV) being the primary causative agent of spring outbreaks leading to massive mortality and economic losses. Although the YcCV genome has been fully characterized, the immune response mechanisms of <em>P. fulvidraco</em> during infection remain poorly understood. Here, we conducted histopathological and transcriptomic analyses of head kidney from YcCV-infected fish at 1 and 5 days post-infection (dpi). Histopathological analysis revealed severe tissue damage, including liver coagulative necrosis, splenic hemorrhage, and widespread inflammatory infiltration in the kidney and heart. Compared to controls, 142 and 3402 differentially expressed genes (DEGs) were identified at 1 dpi and 5 dpi, respectively. GO and KEGG enrichment analyses revealed these DEGs were predominantly involved in signal transduction, cellular growth, apoptosis, immune responses, and metabolic processes. Notably, significant upregulation of <em>IL4i1</em> suggested its potential role in modulating host immunity to control viral replication. siRNA-mediated knockdown of <em>IL4i1</em> in yellow catfish brain cells suppressed YcCV replication, as evidenced by reduced viral load and mRNA levels, indicating a proviral regulatory function of <em>IL4i1</em>. Collectively, these findings provide novel insights into virus-host interactions, advancing our understanding of YcCV pathogenesis and informing future antiviral strategies.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"172 ","pages":"Article 105503"},"PeriodicalIF":2.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145408435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pentraxin family members of largemouth bass (Micropterus salmoides): Cloning, characterization and expression responses to LPS, Poly (I:C) and Nocardia seriolae","authors":"Yawen Yang ,&nbsp;Yushuai Xie ,&nbsp;Zhaosheng Sun ,&nbsp;Zihan Zhang ,&nbsp;Chuanguo Cai ,&nbsp;Zhitao Qi ,&nbsp;Qian Gao","doi":"10.1016/j.dci.2025.105475","DOIUrl":"10.1016/j.dci.2025.105475","url":null,"abstract":"<div><div>Pentraxins (PTXs) are highly conserved soluble pattern recognition molecules, and play important roles in the innate immunity and autoimmune diseases. In present study, ten PTXs including CRP1, SAP, NPTX1-like, NPTX2a, NPTX2b, NPTXR-like, NPTXRb, PTX3a, PTX3-like and PTX4 were identified from largemouth bass (<em>Micropterus salmoides</em>). These PTXs exhibited high sequence identities with their counterparts of other fish species, and contained conserved motifs and structures of the PTX family, except of PTX3-like. Phylogenetic analysis revealed that these largemouth bass PTXs were closely clustered with their counterparts of other fish species, confirming the correctness of the obtained sequences. Realtime qPCR analysis showed that these PTX genes were widely expressed in all examined tissues, and lipopolysaccharide (LPS), Poly (I:C), and <em>Nocardia seriolae</em> stimulation significantly induced their expressions in head kidney (HK), spleen, gill, and intestine. These results demonstrated the crucial roles of PTXs members in the immune response of largemouth bass against pathogen invasion. To the best of our knowledge, this study represents the first identification of NPTX1, NPTX2b, NPTXRa, NPTXRb, and PTX4 in fish species, as well as the initial documentation of PTXs in largemouth bass.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"172 ","pages":"Article 105475"},"PeriodicalIF":2.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145185001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}