Mg2+ inhibits infection of spring viraemia of carp virus (SVCV) by enhancing IRF3 expression

IF 2.4 3区农林科学 Q1 FISHERIES

Developmental and comparative immunology Pub Date : 2025-06-22 DOI:10.1016/j.dci.2025.105403

Na Xu , Xue-Li Wang , Zhuo-Cong Li , Xiao Xu , Bao Jie Cui , Can Zhang , Yang-Yang Wang , Yue Wu , Xiao-Li Yang , Dan-Dan Chen , Long-Feng Lu , Shun Li

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引用次数: 0

Abstract

Magnesium ions (Mg²⁺), essential divalent cations, play a pivotal role in orchestrating a myriad of physiological and biochemical processes. However, the intricate relationship between Mg²⁺ homeostasis and antiviral immunity in fish remains largely unexplored. In this study, we found that Mg²⁺ enhances the interferon (IFN) response by increasing the expression of IFN regulatory factor 3 (IRF3) to inhibit spring viraemia of carp virus (SVCV) infection. In vivo studies showed Mg²⁺ increases the survival rate of SVCV-infected zebrafish while reducing viral transcription and load in liver tissues. Consistent results were obtained in vitro. Further investigations revealed that Mg²⁺ can enhance the endogenous expression of IRF3. Additionally, upon SVCV infection, Mg²⁺ rescues the virus-induced degradation of IRF3. These findings underscore the pivotal role of Mg²⁺ in the antiviral response of lower vertebrates, presenting a promising avenue for addressing fish viral diseases.

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Mg2+通过增强IRF3的表达抑制鲤春病毒血症的感染。

镁离子（Mg2+）是一种必需的二价阳离子，在多种生理生化过程中起着关键作用。然而，鱼类体内Mg2+稳态与抗病毒免疫之间的复杂关系在很大程度上仍未被探索。在本研究中，我们发现Mg2+通过增加干扰素调节因子3 （IRF3）的表达来增强干扰素（IFN）应答，从而抑制鲤鱼病毒（SVCV）感染的春季病毒血症。体内研究表明，Mg2+可提高svcv感染斑马鱼的存活率，同时降低肝组织中病毒的转录和载量。在体外得到了一致的结果。进一步研究发现，镁离子可以增强内源性IRF3的表达。此外，在SVCV感染后，Mg2+恢复了病毒诱导的IRF3的降解。这些发现强调了Mg2+在低等脊椎动物抗病毒反应中的关键作用，为解决鱼类病毒性疾病提供了一条有希望的途径。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Developmental and comparative immunology 医学-动物学

CiteScore

6.20

自引率

6.90%

发文量

206

审稿时长

49 days

期刊介绍： Developmental and Comparative Immunology (DCI) is an international journal that publishes articles describing original research in all areas of immunology, including comparative aspects of immunity and the evolution and development of the immune system. Manuscripts describing studies of immune systems in both vertebrates and invertebrates are welcome. All levels of immunological investigations are appropriate: organismal, cellular, biochemical and molecular genetics, extending to such fields as aging of the immune system, interaction between the immune and neuroendocrine system and intestinal immunity.