Developmental and comparative immunology最新文献

{"title":"Immunohistochemistry analyses of the abomasal mucosa show differences in cellular-mediated immune responses to Haemonchus contortus infection in resistant and susceptible young lambs","authors":"","doi":"10.1016/j.dci.2024.105259","DOIUrl":"10.1016/j.dci.2024.105259","url":null,"abstract":"<div><p><em>Haemonchus contortus</em> is known for its high pathogenicity in sheep, and the uncontrolled use of anthelmintics resulted in the emergence of multiple drug-resistant populations. Breeding sheep for gastrointestinal nematode resistance is a sustainable alternative to reduce dependence of anthelmintic drugs, and differences in the degree of resistance between breeds have been reported. Here we compare two sheep breeds (Santa Ines and Ile de France), concerning the differences in innate and adaptive immune response involved in the resistance against <em>H. contortus</em> infection. Immunohistochemical analyses of the abomasum were conducted in naïve Santa Ines (n = 14) and Ile de France (n = 12) lambs randomized into four groups: infected Santa Ines (n = 8), non-infected control Santa Ines (n = 6), infected Ile de France (n = 8), and non-infected control Ile de France (n = 4). The infected lambs were initially infected with <em>H. contortus</em> infective larvae at 14 days of age, and multiple infections were conducted every second day until they reached 66 days of age. There was a significant effect (P &lt; 0.001) of the infection with increase in numbers of CD3⁺ T; CD79α+ B; GATA3+ Th2/ILC2; POU2F3+ tuft cells; FOXP3+ T reg; and IgE + cells in the fundus of the abomasal mucosa in both Santa Ines and Ile de France lambs. Nevertheless, the infected Santa Ines lambs presented the highest averages for CD79α+ B; GATA3+ Th2/ILC; IgE + cells; and POU2F3+ tuft cells and there was a significant association of the breed and infection status with regards to POU2F3+ tuft cells, with the highest mean in the infected Santa Ines group. The infected Santa Ines group had three lambs with high degree of resistance and five lambs that showed a moderate infection. Our results suggest a mechanism of synergistic coordination between different immune-cell types in promoting resistance of suckling lambs under <em>H. contortus</em> infection.</p></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142099488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A personal view on developmental and comparative immunology: What, how and why?","authors":"","doi":"10.1016/j.dci.2024.105258","DOIUrl":"10.1016/j.dci.2024.105258","url":null,"abstract":"<div><p>What are the future directions of the fields of developmental and comparative immunology? In thinking through this question as I write, I find myself marvelling at the very long ways that we have come since I began as a PhD student some 50 years ago. I think that we cannot know what technical and theoretical advances will emerge in the future, nor will our initial aims survive the realities of what appears in our sights, often from unexpected directions. I feel that we should not allow what we already know about some well-studied systems to blind us to the wide range of possibilities, and that remaining a humble seeker helps the uptake of new realities. Finally, it would be good to try answering the whole range of questions about developmental and comparative immunology, from what to how to why.</p></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0145305X24001307/pdfft?md5=7d9bbe4febd75389216643f4ccf96d1f&pid=1-s2.0-S0145305X24001307-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142105377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preface to the special issue","authors":"","doi":"10.1016/j.dci.2024.105255","DOIUrl":"10.1016/j.dci.2024.105255","url":null,"abstract":"","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142105380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of IgD and IgT with their expressional analysis following subtype II megalocytivirus vaccination and infection in rock bream (Oplegnathus fasciatus)","authors":"","doi":"10.1016/j.dci.2024.105248","DOIUrl":"10.1016/j.dci.2024.105248","url":null,"abstract":"<div><p>In this study, heavy chain genes of IgD and IgT were sequenced and characterized their gene expression in rock bream (<em>Oplegnathus fasciatus</em>). Rock bream (RB)-IgD cDNA is 3319 bp in length and encodes a leader region, variable domains, a μ1 domain, and seven constant domains (CH1–CH7). A membrane-bound (mIgT) and secretory form (sIgT) of RB-IgT cDNAs are 1902 bp and 1689 bp in length, respectively, and encode a leader region, variable domains, four constant domains (CH1–CH4) and C-terminus. Their predicted 3D-structure and phylogenetic relation were similar to those of other teleost. In healthy fish, RB-IgD and mIgT gene expressions were higher in major lymphoid organs and blood, while RB-sIgT gene was more highly expressed in midgut. IgT expressing cells were detected in melano-macrophage centers (MMC) of head kidney in immunohistochemistry analysis. Under immune stimulation <em>in vitro</em>, RB-IgD and IgT gene expressions were upregulated in head kidney and spleen cells by bovine serum albumin or a rock bream iridovirus (RBIV) vaccine. <em>In vivo,</em> their expressions were significantly upregulated in head kidney, blood, and gill upon vaccination. Especially, RB-mIgT gene expression in head kidney and blood was upregulated at day 3 after vaccination while upregulated at earlier time point of day 1 by challenge with RBIV. This may suggest that memory cells might be produced during the primary response by vaccination and rapidly proliferated by secondary immune response by viral infection. RB-sIgT gene expression was highly upregulated in peripheral blood in vaccinated fish after viral infection, indicating that IgT plays an important role in systemic immune response as well as mucosal immune system. Our findings provide information on the role of RB-IgT in adaptive immunity during vaccination and viral infection in the vaccinated fish.</p></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0145305X24001204/pdfft?md5=49633063daf7b3041ddedf50cefae733&pid=1-s2.0-S0145305X24001204-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142105378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Invertebrate immunology – some thoughts about past and future research","authors":"","doi":"10.1016/j.dci.2024.105256","DOIUrl":"10.1016/j.dci.2024.105256","url":null,"abstract":"","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0145305X24001289/pdfft?md5=10b888bdd1b66f4af1ce65a882f7645f&pid=1-s2.0-S0145305X24001289-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142105379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TRIM103 activates the RLRs pathway to enhance antiviral response by targeting VP5 and VP7","authors":"","doi":"10.1016/j.dci.2024.105254","DOIUrl":"10.1016/j.dci.2024.105254","url":null,"abstract":"<div><p>Grass carp (<em>Ctenopharyngodon idella</em>), crucial to global inland aquaculture with a production of 5.8 million tones in 2020, faces significant challenges from hemorrhagic disease caused by grass carp reovirus (GCRV). Rapid mutations compromise current vaccines, underscoring the need for a deeper understanding of antiviral mechanisms to enhance molecular marker-assisted selection. This study investigates the role of Tripartite Motif (TRIM) family in the innate immune response of grass carp, focusing on TRIM103 from <em>Ctenopharyngodon Idella</em> (<em>Ci</em>TRIM103), a member of the TRIM-B30.2 family, which includes proteins with the B30.2 domain at the N-terminus, known for antiviral properties in teleosts. <em>Ci</em>TRIM103 bind to the outer coat proteins VP5 and VP7 of GCRV. This binding is theorized to strengthen the function of the RIG-I-like Receptor (RLR) signaling pathway, crucial for antiviral responses. Demonstrations using overexpression and RNA interference (RNAi) techniques have shown that <em>Ci</em>TRIM103 effectively inhibits GCRV replication. Moreover, molecular docking and pulldown assays suggest potential binding interactions of <em>Ci</em>TRIM103's B30.2 domain with GCRV outer coat proteins VP5 and VP7. These interactions impede viral replication, enhance RLR receptor expression, and activate key transcription factors to induce type I interferons (IFNs). These findings elucidate the antiviral mechanisms of <em>Ci</em>TRIM103, provide a foundation for future Molecular genetic breeding in grass carp.</p></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142105382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The future of comparative immunology","authors":"","doi":"10.1016/j.dci.2024.105240","DOIUrl":"10.1016/j.dci.2024.105240","url":null,"abstract":"<div><p>« Prediction is very difficult, especially if it is about the future of comparative immunology” could one say to paraphrase Niels Bohr. Yet, if one avoids mistakes of the past and fashions, if one remains ready to welcome surprises an do not to get drowned in big data while profiting from new technologies, if one keeps common sense between expanding and restricting one's scope of investigation in front of the enormous diversity of the tree of life, comparative immunologists are going, in new areas of research and with new tools, to keep contributing enormously to immunology. They will reveal, with the eyes open to homologies and analogies among multiple species, more variations on the theme of immunity and will put the human immune system in perspective a necessary situation to face the questions that remain to be answered in order to improve health or to understand evolution of immune systems. There will always be room in comparative immunology for fundamental approaches to these subjects. A proper education, aimed at combining competences, will be essential to achieve these goals.</p></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0145305X24001125/pdfft?md5=c3994471c161dde5912cfd13b10cf57e&pid=1-s2.0-S0145305X24001125-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142055217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SRP54 of black carp negatively regulates MDA5-mediated antiviral innate immunity","authors":"","doi":"10.1016/j.dci.2024.105252","DOIUrl":"10.1016/j.dci.2024.105252","url":null,"abstract":"<div><p>Signal Recognition Particle 54 kDa (SRP54) is a subunit of the signal recognition particle (SRP), a cytoplasmic ribonucleoprotein complex guiding the transportation of newly synthesized proteins from polyribosomes to endoplasmic reticulum. In mammals, it has been reported to regulate the RLR signaling pathway negatively by impairing the association between MAVS and MDA5/RIG-I. However, the role of SRP54 in teleost antiviral innate immune response remains obscure. In this study, the SRP54 homolog of black carp (bcSRP54) has been cloned, and its function in antiviral innate immunity has been elucidated. The CDS of <em>bcSRP54</em> gene consists of 1515 nucleotides and encodes 504 amino acids. Immunofluorescence (IF) showed that bcSRP54 was mainly distributed in the cytoplasm. Overexpressed bcSRP54 significantly reduced bcMDA5-mediated transcription of interferon (IFN) promoter in reporter assay. Co-expression of bcSRP54 and bcMDA5 significantly suppressed bcMDA5-mediated IFN signaling and antiviral activity, while bcSRP54 knockdown increased the antiviral ability of host cells. In addition, the results of the immunofluorescence staining demonstrated the subcellular overlapping between bcSRP54 and bcMDA5, and the co-immunoprecipitation (co-IP) experiment identified their association. Furthermore, the over-expression of bcSRP54 did not influence the protein expression and ubiquitination modification level of bcMDA5, however, hindered the binding of bcMDA5 to bcMAVS. In summary, our results conclude that bcSRP54 targets bcMDA5 and inhibits the interaction between bcMDA5 and bcMAVS, thereby negatively regulating antiviral innate immunity, which provides insight into how teleost SRP54 regulates IFN signaling.</p></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142035524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dragon fruit-derived oligosaccharides alter hemocyte-mediated immunity and expression of genes related to innate immunity and oxidative stress in Daphnia magna","authors":"","doi":"10.1016/j.dci.2024.105251","DOIUrl":"10.1016/j.dci.2024.105251","url":null,"abstract":"<div><p>Dragon fruit oligosaccharide (DFO) is an indigestible prebiotic that enhances the growth and reproduction of <em>Daphnia magna</em>, increases the expression of genes involved in immunity, and reduces oxidative stress. This study investigated the effects of DFO on the expression of innate immunity- (<em>Toll</em>, <em>Pelle</em>, <em>proPO</em>, <em>A2M</em>, and <em>CTL</em>), oxidative stress- (<em>Mn-SOD</em>), and nitric oxide (NO) synthesis-related genes (<em>NOS1</em>, <em>NOS2</em>, and <em>arginase</em>) as well as NO localization and number of hemocytes in <em>D</em>. <em>magna</em>. For this ten-day-old <em>D. magna</em> were treated with 0 or 9 mg l⁻¹ of DFO for 24 and 85 h. Gene expression levels, NO intensity and localization, and total hemocytes were evaluated. After 24 h, the expression of <em>Toll</em> and <em>proPO</em> increased significantly (p &lt; 0.05), while that of C-type lectins (CTL) was reduced (p &lt; 0.05). At 85 h, <em>Mn-SOD</em> and <em>CTL</em> expressions were markedly suppressed (p &lt; 0.05). NO was mostly localized in the foregut, midgut, hindgut, and carapace. The expression of <em>NOS1</em> was reduced after 24 h (p &lt; 0.05). In addition, NO intensity at 24 h was insignificantly lower than the control (p &gt; 0.05). At 85 h, the expression of <em>NOS1, NOS2</em>, and <em>arginase</em> was higher than control, but NO intensity did not differ significantly (p &gt; 0.05). Furthermore, the total hemocyte count elevated remarkably at 85 h (p &lt; 0.05). Our study suggested that 9 mg l⁻¹ of DFO could alter the expression of the genes related to innate immunity, oxidative stress, and NO synthesis in <em>D. magna</em> and significantly stimulate hemocyte production.</p></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142016692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular characterization and gene expression of pattern recognition receptors in brown-marbled grouper (Epinephelus fuscoguttatus) fingerlings responding to vibriosis infection","authors":"","doi":"10.1016/j.dci.2024.105253","DOIUrl":"10.1016/j.dci.2024.105253","url":null,"abstract":"<div><p>The pathogen recognition system involves receptors and genes that play a crucial role in activating innate immune response in brown-marbled grouper (<em>Epinephelus fuscoguttatus</em>) as a control agent against various infections including vibriosis. Here, we report the molecular cloning of partial open reading frames, sequences characterization, and expression profiles of Pattern Recognition Receptors (PRRs) in brown-marbled grouper. The PRRs, namely <em>pglyrp5</em>, <em>tlr5</em>, <em>ctlD,</em> and <em>ctlE</em> in brown-marbled grouper, possess conserved domains and showed shared evolutionary relationships with other fishes, humans, mammals, birds, reptilians, amphibians, and insects. In infection experiments, up to 50% mortality was found in brown-marbled grouper fingerlings infected with <em>Vibrio alginolyticus</em> compared to 27% mortality infected <em>Vibrio parahaemolyticus</em> and 100% survival of control groups. It is also demonstrated that all four PRRs had higher expression in samples infected with <em>V. alginolyticus</em> compared to <em>V. parahaemolyticus</em>. This PRRs gene expression analysis revealed that all four PRRs expressed rapidly at 4-h post-inoculation even though the <em>Vibrio</em> count was only detected earliest at 12-h post-inoculation in samples. The highest expression recorded was from <em>V</em>. <em>alginolyticus</em> inoculated fish spleen with up to 73-fold change for <em>pglyrp5</em> gene, followed by 14 to 38-fold expression for the same treatment in spleen, head kidney, and blood samples for other PRRs, namely <em>tlr5</em>, <em>ctlD,</em> and <em>ctlE</em> genes. Meanwhile less than a 10% increase in expression of all four genes was detected in spleen, head kidney, and blood samples inoculated with <em>V. parahaemolyticus</em>. These findings indicated that <em>pglyrp5</em>, <em>tlr5</em>, <em>ctlD,</em> and <em>ctlE</em> play important roles in the early immune response to vibriosis infected, brown-marbled grouper fingerlings.</p></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142016693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}