Developmental and comparative immunology最新文献

{"title":"Infectious myonecrosis virus (IMNV) induces upregulation of RNAi-related genes in white shrimp Penaeus vannamei.","authors":"Rubens Galdino Feijó, Jhonatas Teixeira Viana, Rodrigo Maggioni, Luis Fernando Marins","doi":"10.1016/j.dci.2024.105296","DOIUrl":"10.1016/j.dci.2024.105296","url":null,"abstract":"<p><p>Infectious myonecrosis virus (IMNV) still causes significant economic and social losses in American and Asian shrimp farming. In this work, we investigated the transcription patterns of Sid-1, Dicer-2 and Argonaute-2 genes from the RNAi mechanism in Penaeus vannamei naturally infected with IMNV, and injected with inoculum containing 1.02 × 10⁵, 1.02 × 10⁴ or 1.02 × 10³ IMNV copies‧μL^-1. We observed that infection with increasing IMNV concentrations affected the transcription levels of these key genes. However, the viral load did not decrease during the experiment. We suggest that changes in Sid-1 mRNA expression could be used as marker of viral replication for evaluating sanitary status in P. vannamei farming.</p>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":" ","pages":"105296"},"PeriodicalIF":2.7,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142779269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome-wide identification and characterization of Toll pathway genes in Riptortus pedestris (Hemiptera: Alydidae)","authors":"Yipeng Ren ,&nbsp;Wenhao Dong ,&nbsp;Juhong Chen ,&nbsp;Wenjun Bu ,&nbsp;Huaijun Xue","doi":"10.1016/j.dci.2024.105294","DOIUrl":"10.1016/j.dci.2024.105294","url":null,"abstract":"<div><div>The Toll pathway was first identified in <em>Drosophila</em> and plays an essential role in defense against infection by various pathogens. To date, various noncoding RNAs (ncRNAs) have been demonstrated to maintain immune homeostasis by regulating several target genes in the insect Toll pathway. However, the characterization and function of Toll pathway genes involved in the response to environmental changes at the posttranscriptional level associated with gut bacterial changes in <em>Riptortus pedestris</em>, which is a significant pest of soybeans, remain unclear. In this study, we identified and classified six <em>Toll</em> genes into three subtypes with typical Toll domain arrangements, including a Toll/interleukin receptor (TIR) domain, a transmembrane domain, and multiple leucine-rich repeat (LRR) domains; in addition, only one positive selection site was found in hemipteran sPP-Tolls, and a total of five downstream members in the Toll signaling pathway were selected and characterized. The expression patterns revealed that all these genes were widely expressed at all developmental stages of <em>R. pedestris</em>, and they presented variable expression levels among the different feeding treatments in the <em>R. pedestris</em> gut. Our comprehensive prediction analysis revealed that there are sixty miRNA‒mRNA interaction pairs, including fifty-six miRNA and six Toll pathway genes (<em>P‒Toll1</em>, <em>sP‒Toll</em>, <em>Myd88</em>, <em>Pelle</em>, <em>Tube</em>, and <em>Cactus</em>), and a ceRNA network comprising two lncRNA‒miRNA‒Toll pairs was constructed in response to environmental changes. Finally, the expression of some above genes and ncRNAs from the ceRNA network exhibited positive or negative association with the most changes in gut bacterial genera via Pearson correlation analysis. These findings provide valuable insights into how the Toll pathway of <em>R. pedestris</em> is involved in environmental adaptation at the posttranscriptional level and identifies new avenues for developing more effective methods for pest control through integration with gut bacteria.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"162 ","pages":"Article 105294"},"PeriodicalIF":2.7,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142759412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CgANT2 regulates mitophagy of oyster haemocyte response against bacterial stimulation","authors":"Wei Wu ,&nbsp;Xiaoqian Lv ,&nbsp;Jiejie Sun ,&nbsp;Zihan Wang ,&nbsp;Miren Dong ,&nbsp;Lingling Wang ,&nbsp;Linsheng Song","doi":"10.1016/j.dci.2024.105295","DOIUrl":"10.1016/j.dci.2024.105295","url":null,"abstract":"<div><div>Adenine nucleotide translocator (ANT) is a major molecule in the inner membrane of mitochondria that plays an important role in regulating mitophagy. In the present study, a conserved ANT2 homologue (designated as <em>Cg</em>ANT2) was identified and functionally characterized in oyster <em>Crassostrea gigas</em>. There were three typical Mito_carr tandem repeats in <em>Cg</em>ANT2. The mRNA expression levels of <em>Cg</em>ANT2 in haemocytes increased significantly at 24 and 72 h after <em>Vibrio splendidus</em> stimulation. Its protein was abundantly expressed in granulocytes and was observed to be colocalized with mitochondria. When <em>Cg</em>ANT2 expression was suppressed by injection with its dsRNA, there was an increased mitochondrial reactive oxygen species (mtROS) production and mitochondrial permeability transition pore (mPTP) opening, while the mRNA expression levels of mitophagy-related genes (<em>Cg</em>PINK1 and <em>Cg</em>Parkin) and the percentage of mitophagy in haemocytes all decreased significantly. These results indicated that <em>Cg</em>ANT2 regulated mtROS production and mPTP opening, thereby inducing mitophagy in the oyster haemocyte response against <em>V. splendidus</em> stimulation.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"162 ","pages":"Article 105295"},"PeriodicalIF":2.7,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142754770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Parkin is a critical factor in grouper immune response to virus infection","authors":"Xiaoxia Lei ,&nbsp;Siting Wu ,&nbsp;Zhuqing Xu ,&nbsp;Qiongyue Xu ,&nbsp;Helong Cao ,&nbsp;Zhouling Zhan ,&nbsp;Qiwei Qin ,&nbsp;Jingguang Wei","doi":"10.1016/j.dci.2024.105293","DOIUrl":"10.1016/j.dci.2024.105293","url":null,"abstract":"<div><div>Parkin is an E3 ubiquitinated ligase that mainly participates in mitophagy and plays an essential biological role in organisms. To investigate Parkin's function in fish, a Parkin homolog was cloned from <em>Epinephelus coioides</em> (EcParkin). The open reading frame (ORF) of EcParkin consists of 1461 nucleotides and encodes a protein of 486 amino acids, with a predicted molecular weight of 53.32 kDa. EcParkin was highly expressed in the heart, kidney, and head kidney of healthy groupers, especially in the heart. The expression levels of EcParkin were upregulated after Singapore grouper iridovirus (SGIV) and red-spotted grouper nervous necrosis virus (RGNNV) infection. Intracellular localization studies revealed that EcParkin is distributed in both the cytoplasm and nucleus of GS cells. Overexpression of EcParkin promoted SGIV and RGNNV replication <em>in vitro</em>, while knockdown of EcParkin inhibited SGIV and RGNNV replication. EcParkin suppressed the promoter activities of IFN-β, ISRE, and NF-κB, as well as the expression of interferon-related factors and inflammatory cytokines. EcParkin was found to colocalize and interact with EcMDA5, EcMAVS, EcTBK1, EcIRF3, and EcIRF7. Additionally, EcParkin enhanced LC3-II production in GS cells. These findings suggest that EcParkin may play a crucial role in the antiviral innate immunity and cellular autophagy of fish.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"162 ","pages":"Article 105293"},"PeriodicalIF":2.7,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142746448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of the potential long-lasting effects of probiotic Debaryomyces hansenii CBS 8339 on trained immunity in newborn goats","authors":"Miriam Angulo ,&nbsp;Carlos Angulo","doi":"10.1016/j.dci.2024.105292","DOIUrl":"10.1016/j.dci.2024.105292","url":null,"abstract":"<div><div>Trained immunity has been described as the memory capacity of the innate immune system. Several microbial components have been shown to induce trained immunity. Research on the potential of probiotics to trigger these effects has been limited to a few <em>in vitro</em> studies but remains completely unknown <em>in vivo</em>. Components from the probiotic <em>Debaryomyces hansenii</em> CBS 8339 (Dh) have been shown to induce innate immune memory in goat kids and calves. In the present study, stimulating innate immune cells from newborn goats with probiotic Dh increased respiratory burst activity and nitric oxide production, while cell phagocytosis was unaffected. Glucose uptake was enhanced in goat's cells stimulated with Dh, but lactate production was decreased. In newborn goats, after the training scheme (via oral probiotic administration), cell phagocytosis, nitric oxide production and glycolysis — through the upregulation of <em>AKT</em> and <em>HIF1A</em> gene expression, glucose consumption and lactate production— were enhanced. The expression of <em>IL1B</em> gene was similar between the <em>D. hansenii</em> and control groups. Moreover, the potential long-lasting effects were assessed 30 days after initiation of the training scheme. Cell phagocytosis, respiratory burst and myeloperoxidase activity were enhanced, while glycolytic parameters remained unaffected. Altogether, the results of the present study suggest that the immune training scheme may induce trained immunity by the probiotic <em>D. hansenii</em> in newborn goats. However, our findings were not conclusive regarding the long-lasting (one-month) effects of trained immunity by probiotics.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"162 ","pages":"Article 105292"},"PeriodicalIF":2.7,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142692558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of a novel clonal GFP-expressing transgenic ginbuna crucian carp","authors":"Ren Uehara ,&nbsp;Shinji Takeda ,&nbsp;Daichi Oku ,&nbsp;Ryo Sasaki ,&nbsp;Masaru Murakami ,&nbsp;Hajime Shiba ,&nbsp;Fumihiko Katakura ,&nbsp;Tadaaki Moritomo","doi":"10.1016/j.dci.2024.105290","DOIUrl":"10.1016/j.dci.2024.105290","url":null,"abstract":"<div><div>The clonal triploid ginbuna crucian carp <em>Carassius auratus langsdorfii</em>, a naturally occurring gynogenetic fish, is suitable for cell transplantation studies to reveal the roles of stem cells and immune cells. To ensure long-term traceability of donor cells within recipient fish, we have established a transgenic ginbuna line that expresses green fluorescent protein (GFP). The <em>Xenopus laevis ef1a</em> promoter was introduced for regulating GFP expression. <em>Tol2</em> transposon-based transgenesis to ginbuna embryos resulted in producing a putative founder fish (F0) in a mosaic fluorescent fashion; the frequency of germline transmission was 14.9%. All embryos of GFP-positive offspring (F1)-derived F2 generation expressed GFP widely across the body. The result of Southern blot analysis showed that the transgene was present on a single DNA fragment of equivalent size among F1 and F2 individuals tested, indicating that the transgene was stably transmitted without translocation. Analysis of the fluorescence intensity of organs obtained from F1 and F2 juveniles using fluorescence microscope showed that eyes, brain, skeletal muscle, heart and gonad exhibited a strong GFP fluorescence while gill, spleen and intestine gave a weak signal; no fluorescence was observed in erythrocytes. Flow cytometric analyses of peripheral leukocytes from F1 and F2 adult fish revealed all cell populations expressed GFP. Scale grafts from the transgenic fish to the wild-type fish exhibited persistent engraftment. Together, our transgenic line can be a powerful tool for studying cellular dynamics by cell transplantation and provide a solid basis for further immunological research advances in teleost.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"162 ","pages":"Article 105290"},"PeriodicalIF":2.7,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142686405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First report of Peroxiredoxin-5 (PRDX5) in starry flounder (Platichthys stellatus): Molecular features and expression analysis.","authors":"Min-Young Sohn, Kwang-Min Choi, Ji-Min Jeong, Gyoungsik Kang, Won-Sik Woo, Kyung-Ho Kim, Ha-Jeong Son, Hye-Seong Kim, Jun-Ichi Hikima, Chan-Il Park","doi":"10.1016/j.dci.2024.105291","DOIUrl":"10.1016/j.dci.2024.105291","url":null,"abstract":"<p><p>In this study, the Peroxiredoxin-5 (PRDX5) gene from starry flounder (Platichthys stellatus, PsPRDX5) was identified through next-generation sequencing (NGS) and verified via cloning and sequencing. The predicted amino acid sequence, derived from the cDNA, was confirmed by multiple sequence alignment and phylogenetic analysis, establishing its homology with related sequences. PsPRDX5 mRNA was expressed across all examined tissues in healthy starry flounder, with distinct tissue-specific expression profiles. Following artificial infection with viral hemorrhagic septicemia virus (VHSV) and Streptococcus parauberis PH0710, significant alterations in PsPRDX5 mRNA expression were observed. VHSV infection resulted in a pronounced downregulation of PsPRDX5 in most major tissues, except for a significant upregulation in the intestine at 7 days post-infection. Conversely, infection with S. parauberis PH0710 led to a significant upregulation of PsPRDX5 in nearly all tissues. These results suggest that the differential expression of PsPRDX5 in response to VHSV and S. parauberis PH0710 reflects pathogen-specific immune and antioxidant defense mechanisms, highlighting the role of PsPRDX5 in the immune response of starry flounder to both viral and bacterial infections.</p>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":" ","pages":"105291"},"PeriodicalIF":2.7,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142686407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A recombinant sPLA2 protein promotes gut mucosal barrier against bacterial infection in fish","authors":"Qiongyao Zeng ,&nbsp;Yiyang Tang ,&nbsp;Yujun Liu ,&nbsp;Ye Yang ,&nbsp;Pingyuan Li ,&nbsp;Zejun Zhou ,&nbsp;Qinbo Qin","doi":"10.1016/j.dci.2024.105288","DOIUrl":"10.1016/j.dci.2024.105288","url":null,"abstract":"<div><div>Secreted phospholipase A2 family protein (sPLA2) is associated with immune response and plays a critical role in the regulation of gut homeostasis. However, whether sPLA2 is involved in innate immunity in teleost is essentially unknown. For this purpose, we reported the identification of a classical sPLA2 in grass carp (<em>Ci</em>sPLA2) and elucidated its role in the antibacterial immunity in this study. The result of bioinformatics analysis showed that mammalian sPLA2-IIA is the most similar homologue to <em>Ci</em>sPLA2. <em>CisPLA2</em> is expressed in a variety of tissues, including liver and gut, and is significantly upregulated in response to <em>Aeromonas hydrophila</em> infection. Recombinant <em>Ci</em>sPLA2 protein (r<em>Ci</em>sPLA2) showed significant antibacterial activity against <em>A</em>. <em>hydrophila</em> by enhancing the phagocytosis of host phagocytes <em>in vitro</em>. Moreover, r<em>Ci</em>sPLA2 induces significant expression of the antimicrobial molecules and tight junctions in the gut during bacterial infection. Fish administered with r<em>Ci</em>sPLA2 significantly alleviates the gut permeability and apoptosis. In addition, r<em>Ci</em>sPLA2 preserves the morphology of the gut mucosa and limits the colonization of <em>A</em>. <em>hydrophila</em> in systemic immune organs. These results indicate that <em>Ci</em>sPLA2 plays a crucial role in the regulation of gut mucosal barrier, and thus has a potential application for antimicrobial immunity in fish.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"162 ","pages":"Article 105288"},"PeriodicalIF":2.7,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142616551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zebrafish use conserved CLR and TLR signaling pathways to respond to fungal PAMPs in zymosan","authors":"Erin Glass ,&nbsp;Stephan L. Robinson ,&nbsp;Emily E. Rosowski","doi":"10.1016/j.dci.2024.105286","DOIUrl":"10.1016/j.dci.2024.105286","url":null,"abstract":"<div><div>Pattern recognition receptors (PRRs) such as C-type lectin receptors (CLRs) and Toll-like receptors (TLRs) are used by hosts to recognize pathogen-associated molecular patterns (PAMPs) in microorganisms and to initiate innate immune responses. While PRRs exist across invertebrate and vertebrate species, the functional homology of many of these receptors is still unclear. In this study, we investigate the innate immune response of zebrafish larvae to zymosan, a β-glucan-containing particle derived from fungal cell walls. Macrophages and neutrophils robustly respond to zymosan and are required for zymosan-induced activation of the NF-κB transcription factor. Full activation of NF-κB in response to zymosan depends on Card9/Syk and Myd88, conserved CLR and TLR adaptor proteins, respectively. Two putative CLRs, Clec4c and Sclra, are both required for maximal sensing of zymosan and NF-κB activation but not required for inflammatory gene expression. Altogether, we identify conserved PRRs and PRR signaling pathways in larval zebrafish that promote recognition of fungal PAMPs. These results inform modeling of human fungal infections in zebrafish and increase our knowledge of the evolution and conservation of PRR pathways in vertebrates.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"162 ","pages":"Article 105286"},"PeriodicalIF":2.7,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142616554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional characterization of peroxiredoxin 5 from yellowtail clownfish (Amphiprion clarkii): Immunological expression assessment, antioxidant activities, heavy metal detoxification, and nitrosative stress mitigation","authors":"D.C.G. Rodrigo ,&nbsp;H.M.V. Udayantha ,&nbsp;D.S. Liyanage ,&nbsp;W.K.M. Omeka ,&nbsp;Y.K. Kodagoda ,&nbsp;H.A.C.R. Hanchapola ,&nbsp;M.A.H. Dilshan ,&nbsp;G.A.N.P. Ganepola ,&nbsp;W.A.D.L.R. Warnakula ,&nbsp;Gaeun Kim ,&nbsp;Jeongeun Kim ,&nbsp;Jihun Lee ,&nbsp;Qiang Wan ,&nbsp;Jehee Lee","doi":"10.1016/j.dci.2024.105289","DOIUrl":"10.1016/j.dci.2024.105289","url":null,"abstract":"<div><div>Peroxiredoxin 5 (<em>Prdx5</em>) is the last recognized member of Prdx family. It is a unique, atypical, 2-Cys antioxidant enzyme, protecting cells from death caused by reactive oxygen species (ROS). In this study, the Prdx5 ortholog of <em>Amphiprion clarkii</em> (<em>AcPrdx5</em>) was identified and characterized to explore its specific structural features and functional properties. The open reading frame of AcPrdx5 is 573 bp long and encodes 190 amino acids containing a mitochondrial targeting sequence, thioredoxin domain, and two conserved cysteine residues responsible for antioxidant function. The predicted molecular weight and theoretical isoelectric point of AcPrdx5 are 20.3 kDa and 9.01, respectively. AcPrdx5 sequences were found to be highly conserved across the other orthologs from various organisms and it distinctively clustered within the fish <em>Prdx5</em> subclade of the phylogenetic tree. The expression of <em>AcPrdx5</em> was ubiquitously detected among twelve tested tissues, with the highest level in the brain. Furthermore, the mRNA levels of <em>AcPrdx5</em> in the blood and head-kidney tissues were significantly (<em>p &lt; 0.05</em>) upregulated following polyinosinic-polycytidylic acid (Poly I:C), lipopolysaccharide (LPS), and <em>Vibrio harveyi</em> immune challenge<em>.</em> A concentration-dependent antioxidant potential of recombinant AcPrdx5 was observed in insulin disulfide bond reduction, heavy metal detoxification, free radical and hydrogen peroxide (H₂O₂) scavenging assays. Additionally, AcPrdx5 overexpression in fathead minnow (FHM) cells upregulated the antioxidant-associated gene (Rrm1, MAPK, SOD2, and PRDX1) expression after H₂O₂ treatment, and promoted cell viability upon arsenic (As) exposure. In macrophages, AcPrdx5 overexpression effectively suppressed substantial nitric oxide production under lipopolysaccharide treatment. Collectively, our results suggest that AcPrdx5 may play roles in both antioxidant defense system and innate immune response against pathogenic invasions in <em>A</em>. <em>clarkii</em>.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"162 ","pages":"Article 105289"},"PeriodicalIF":2.7,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142616552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}