Putative orthologues of natural killer cell receptors and ligands in shrimp

Abstract

In vertebrates, natural killer (NK) cells play an important role in innate immunity. They detect target cells by receptor-ligand interactions and become activated or inhibited by a variety of cell surface activating and inhibitory receptors. In invertebrates, the defense against pathogens relies entirely on innate immunity, of which haemocytes are the only immune cells. In shrimp, two subpopulations of non-adherent, non-phagocytic, lymphocyte-like haemocytes were identified to have an NK-like activity. It is postulated now that they also may use the NK-like recognition mechanism by receptors and ligands. By searching for NK receptor genes in the shrimp genome database (already identified and described as such or by aligning the sequence of mammalian NK receptors with the shrimp genome), several receptors, such as tumor necrosis factor receptor superfamily (TNFRSF), semaphorins (SEMAs), sialic acid-binding immunoglobulin-like lectins (SIGLECs) and their corresponding ligands were discovered. The domain structure of these receptors and ligands were described and compared with those of mammalian domains, revealing 13 %–64 % similarity between them. The identification of shrimp NK cell surface receptors and their signaling pathways is primarily summarized in this review. Understanding the molecular interactions between NK-like receptors and their corresponding ligands in shrimp will help enhance our understanding of invertebrate immunology and controlling infectious diseases in shrimp aquaculture.