Cytogenetics and cell genetics最新文献_第6页

Chromosomal localization of the genes encoding ALDH, BMP-2, R-FABP, IFN-gamma, RXR-gamma, and VIM in chicken by fluorescence in situ hybridization. 荧光原位杂交技术在鸡染色体上定位ALDH、BMP-2、R-FABP、ifn - γ、rxr - γ和VIM基因。

Cytogenetics and cell genetics Pub Date : 2000-01-01 DOI: 10.1159/000015535

M Guttenbach, I Nanda, P M Brickell, R Godbout, P Staeheli, Z E Zehner, M Schmid

引用次数: 14

Assignment of type I phosphatidylinositol-4-phosphate 5-kinase (PIP5K1A) to human chromosome bands 1q22--> q24 by in situ hybridization. I型磷脂酰肌醇-4-磷酸5激酶(PIP5K1A)在人类染色体带1q22- > q24上的原位杂交。

Cytogenetics and cell genetics Pub Date : 2000-01-01 DOI: 10.1159/000015545

Y Xie, L Zhu, G Zhao

引用次数: 2

cDNA cloning and genomic structure of a novel gene (C11orf9) localized to chromosome 11q12-->q13.1 which encodes a highly conserved, potential membrane-associated protein. 定位于染色体11q12- >q13.1的新基因C11orf9的cDNA克隆和基因组结构，该基因编码高度保守的潜在膜相关蛋白。

Cytogenetics and cell genetics Pub Date : 2000-01-01 DOI: 10.1159/000015552

H Stöhr, A Marquardt, K White, B H Weber

{"title":"cDNA cloning and genomic structure of a novel gene (C11orf9) localized to chromosome 11q12-->q13.1 which encodes a highly conserved, potential membrane-associated protein.","authors":"H Stöhr, A Marquardt, K White, B H Weber","doi":"10.1159/000015552","DOIUrl":"https://doi.org/10.1159/000015552","url":null,"abstract":"We have cloned and characterized a novel gene (C11orf9) mapping to chromosome 11q12-->q13.1. The transcript was initially identified as a partial cDNA sequence in the course of constructing a transcript map of the region between markers D11S1765 and uteroglobin known to encompass the gene causing Best disease. Using a combination of EST mapping, computational exon prediction, RT-PCR, and 5'-RACE its 5. 7-kb full-length cDNA sequence was subsequently obtained. The C11orf9 gene consists of 26 exons spanning 33.1 kb of genomic DNA and is located about 4.3 kb centromeric to FEN1. Biocomputational analysis predicts that its conceptual translation product of 1,111 amino acids contains two transmembrane helices as well as two proline-rich regions. Alignment reveals significant homology to hypothetical peptides from several other species including C. elegans and D. melanogaster, indicating a high degree of conservation throughout evolution. Northern Blot and RT-PCR analyses demonstrate widespread expression of a single transcript but varying degrees of abundance among the individual tissues tested. Mutation analysis of the entire coding sequence excluded C11orf9 as the Best disease gene.","PeriodicalId":10982,"journal":{"name":"Cytogenetics and cell genetics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000015552","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21673941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Assignment of seta to distal mouse X chromosome by radiation hybrid mapping. 用放射杂交作图法确定远端小鼠X染色体的位置。

Cytogenetics and cell genetics Pub Date : 2000-01-01 DOI: 10.1159/000015634

M A Hyatt, V W Sykes, A D Boyer, K C Arden, O Bögler

引用次数: 2

cDNA cloning of putative rat acetyl-CoA transporter and its expression pattern in brain. 大鼠乙酰辅酶a转运体cDNA克隆及其脑内表达模式。

Cytogenetics and cell genetics Pub Date : 2000-01-01 DOI: 10.1159/000015613

R S Bora, S Ichikawa, A Kanamori, Y Hirabayashi

引用次数: 7

Comparative mapping of seven genes in mouse, rat and Chinese hamster chromosomes by fluorescence in situ hybridization. 荧光原位杂交对小鼠、大鼠和仓鼠染色体7个基因的比较定位。

Cytogenetics and cell genetics Pub Date : 2000-01-01 DOI: 10.1159/000015614

T Ono, S Hirano, S Yonezawa, S Aono, M Osaki, S Masaki, S Yamashita, T Tsukasaki, A Oohira, S T Suzuki, S Sonta

{"title":"Comparative mapping of seven genes in mouse, rat and Chinese hamster chromosomes by fluorescence in situ hybridization.","authors":"T Ono, S Hirano, S Yonezawa, S Aono, M Osaki, S Masaki, S Yamashita, T Tsukasaki, A Oohira, S T Suzuki, S Sonta","doi":"10.1159/000015614","DOIUrl":"https://doi.org/10.1159/000015614","url":null,"abstract":"By fluorescence in situ hybridization (FISH) using mouse probes, we assigned homologues for cathepsin E (Ctse), protocadherin 10 (Pcdh10, alias OL-protocadherin, Ol-pc), protocadherin 13 (Pcdh13, alias protocadherin 2c, Pcdh2c), neuroglycan C (Cspg5) and myosin X (Myo10) genes to rat chromosomes (RNO) 13q13, 2q24-->q25, 18p12-->p11, 8q32.1 and 2q22.1-->q22.3, respectively. Similarly, homologues for mouse Ctse, Pcdh13, Cspg5 and Myo10 genes and homologues for rat Smad2 (Madh2) and Smad4 (Madh4) genes were assigned to Chinese hamster chromosomes (CGR) 5q28, 2q17, 4q26, 2p29-->p27, 2q112-->q113 and 2q112-->q113, respectively. The chromosome assignments of homologues of Ctse and Cspg5 reinforced well-known homologous relationships among mouse chromosome (MMU) 1, RNO 13 and CGR 5q, and among MMU 9, RNO 8 and CGR 4q, respectively. The chromosome locations of homologues for Madh2, Madh4 and Pcdh13 genes suggested that inversion events were involved in chromosomal rearrangements in the differentiation of MMU 18 and RNO 18, whereas most of MMU 18 is conserved as a continuous segment in CGR 2q. Furthermore, the mapping result of Myo10 and homologues suggested an orthologous segment of MMU 15, RNO 2 and CGR 2.","PeriodicalId":10982,"journal":{"name":"Cytogenetics and cell genetics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000015614","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21800821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

The p53-inducible gene EI24/PIG8 localizes to human chromosome 11q23 and the proximal region of mouse chromosome 9. p53诱导基因EI24/PIG8定位于人类染色体11q23和小鼠染色体9的近端区域。

Cytogenetics and cell genetics Pub Date : 2000-01-01 DOI: 10.1159/000015620

Z Gu, D J Gilbert, V A Valentine, N A Jenkins, N G Copeland, G P Zambetti

引用次数: 17

Assignment of CDK5R2 coding for the cyclin-dependent kinase 5, regulatory subunit 2 (NCK5AI protein) to human chromosome band 2q35 by fluorescent in situ hybridization. CDK5R2编码周期蛋白依赖性激酶5，调控亚基2 (NCK5AI蛋白)通过荧光原位杂交定位到人类染色体2q35带。

Cytogenetics and cell genetics Pub Date : 2000-01-01 DOI: 10.1159/000015602

O V Muravenko, R Z Gizatullin, A I Protopopov, V I Kashuba, E R Zabarovsky, A V Zelenin

{"title":"Assignment of CDK5R2 coding for the cyclin-dependent kinase 5, regulatory subunit 2 (NCK5AI protein) to human chromosome band 2q35 by fluorescent in situ hybridization.","authors":"O V Muravenko, R Z Gizatullin, A I Protopopov, V I Kashuba, E R Zabarovsky, A V Zelenin","doi":"10.1159/000015602","DOIUrl":"https://doi.org/10.1159/000015602","url":null,"abstract":"Neuronal CDC2-like kinase (OMIM: 116940) is a heterodimer of CDK5 (OMIM: 123831) and p25 (nck5a), a neuronspecific 25-kDa regulatory subunit derived proteolytically from NCK5A, encoded by CDK5R1 (neuronal CDK5 activator or cyclin-dependent kinase 5 regulatory subunit 1 gene) (Lew et al., 1994; Tsai et al., 1994). By screening a human hippocampus library with a bovine Nck5a cDNA, Tang et al. (1995) isolated cDNAs encoding NCK5AI, a distinct NCK5A isoform. They also referred to the protein as p39 (nck5ai) based on its calculated molecular mass of 39 kDa. This isoform showed a high degree of sequence similarity to p35 (NCK5A) with 57% amino acid identity. A 30-kDa truncated form of p39 (nck5ai) activated both recombinant and native CDK5 in vitro, as does p25 (nck5a). Northern blot analysis of rat tissues indicated that both NCK5A and p39 (nck5ai) are expressed exclusively in brain. In situ hybridization to rat brain sections revealed that p39 (nck5ai) mRNA was highly expressed in the CA1 to CA3 zone of the hippocampal formation, an area highly enriched in neurons. There was no expression in the fimbria hippocampi, where glial cells predominate. Tang et al. (1995) concluded that p39 (nck5ai) shares many common characteristics with NCK5A, including CDK5-activating activity and brainand neuron-specific expression. Both proteins show limited sequence homology to cyclins, suggesting that they define a new family of cyclin-dependent kinase-activating proteins. The gene encoding p39 (nck5ai) was called CDK5R2. Partial sequencing of the NotI linking clone NR3-007 (Zabarovsky et al., 1994) revealed that it is 100% identical to CDK5R2 over 378 bp (aa 110–235). We concluded that NR3-007 contains part of CDK5R2 and mapped this gene using the NR3-007 clone.","PeriodicalId":10982,"journal":{"name":"Cytogenetics and cell genetics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000015602","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21800883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Characterization and genomic localization of the mouse Extl2 gene. 小鼠Extl2基因的鉴定和基因组定位。

Cytogenetics and cell genetics Pub Date : 2000-01-01 DOI: 10.1159/000015609

W Wuyts, W Van Hul

引用次数: 7

Genomic structure and chromosome mapping of human and mouse RAMP genes. 人类和小鼠RAMP基因的基因组结构和染色体定位。

Cytogenetics and cell genetics Pub Date : 2000-01-01 DOI: 10.1159/000015644

C Derst, H Engel, K Grzeschik, J Daut

引用次数: 14