Spontaneous frequencies of aneuploid and diploid sperm in 10 normal Chinese men: assessed by multicolor fluorescence in situ hybridization.

Q Shi, R H Martin
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引用次数: 38

Abstract

Many studies have been published establishing the background frequencies of disomic and diploid sperm in normal men by fluorescence in situ hybridization (FISH) analysis, with highly significant variance among the reports. Besides interdonor heterogeneity and differences in the experimental protocols used, the question of inherent differences in chromosome malsegregation and meiotic arrest among different geographic and ethnic groups of donors has been raised. In this study, multicolor FISH analysis was carried out on semen samples from 10 nonsmoking, nondrinking Chinese men from the People's Republic of China. The results were compared to FISH data on 10 nonsmoking, nondrinking Canadians under the same experimental conditions, in the same laboratory. A total of 200,497 sperm was scored in the Chinese donors and compared to 202,320 sperm from Canadian donors. Approximately 10,000 sperm per chromosome probe per donor were analyzed. The mean hybridization efficiency was 99.99%. The frequencies of X-bearing and Y-bearing sperm were not significantly different from the expected 50% for each individual and for the combined data from all donors (49.73% vs. 49.46%, P = 0.3946). The mean disomy frequencies (range) were 0.07% (0.02%-0.12%) for chromosome 13, 0.18% (0.09%-0.19%) for chromosome 21, 0.05% (0. 01%-0.09%) for 24,XX, 0.02% (0.01%-0.06%) for 24,YY, and 0.29% (0. 13%-0.49%) for 24,XY. The mean diploidy frequency (range) was 0.38% (0.22%-0.73%) for 13-21 hybridizations and 0.32% (0.07%-0.70%) for XY hybridizations. Highly significant interdonor heterogeneity was found for diploidy (P = 0.0000) and for XY disomy (P = 0.0011), but no age effect was observed in any category of disomic or diploid sperm. The data reported here show no marked differences in disomy and diploidy frequencies between the mainland Chinese and Canadian groups, if donor heterogeneity is taken into account.

10例正常中国男性非整倍体和二倍体精子的自发频率:多色荧光原位杂交评估。
已经发表了许多研究,通过荧光原位杂交(FISH)分析建立了正常男性二体和二倍体精子的背景频率,报告之间存在高度显著的差异。除了供体间的异质性和实验方案的差异外,还提出了不同地理和种族的供体在染色体异常分离和减数分裂停止方面的内在差异问题。在这项研究中,对来自中华人民共和国的10名不吸烟、不饮酒的中国男性的精液样本进行了多色FISH分析。研究结果与FISH数据进行了比较,这些数据来自10名不吸烟、不喝酒的加拿大人,他们在相同的实验条件下,在同一个实验室。中国捐献者的精子总数为200,497个,而加拿大捐献者的精子总数为202,320个。每个捐献者的每个染色体探针大约分析了10,000个精子。平均杂交效率为99.99%。携带x染色体和携带y染色体的精子的频率与预期的每个个体和所有供者的组合数据的50%没有显著差异(49.73%对49.46%,P = 0.3946)。13号染色体的平均二体频率为0.07%(0.02% ~ 0.12%),21号染色体的平均二体频率为0.18%(0.09% ~ 0.19%),21号染色体的平均二体频率为0.05%(0.09% ~ 0.19%)。01% - -0.09%) 24, XX, 0.02%(0.01% - -0.06%) 24日,YY, 0.29%(0。13%-0.49%)。13 ~ 21杂交的平均二倍体倍率为0.38% (0.22% ~ 0.73%),XY杂交的平均二倍体倍率为0.32%(0.07% ~ 0.70%)。二倍体(P = 0.0000)和XY二体(P = 0.0011)的供体间异质性非常显著,但在二体和二倍体精子的任何类别中均未观察到年龄效应。这里报告的数据显示,如果考虑供体异质性,中国大陆和加拿大人群在二体和二倍体频率上没有显著差异。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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