Cytogenetics and cell genetics最新文献_第5页

First report on chicken genes and chromosomes 2000. 鸡基因和染色体首次报道2000。

Cytogenetics and cell genetics Pub Date : 2000-01-01 DOI: 10.1159/000056772

M Schmid, I Nanda, M Guttenbach, C Steinlein, M Hoehn, M Schartl, T Haaf, S Weigend, R Fries, J M Buerstedde, K Wimmers, D W Burt, J Smith, S A'Hara, A Law, D K Griffin, N Bumstead, J Kaufman, P A Thomson, T Burke, M A Groenen, R P Crooijmans, A Vignal, V Fillon, M Morisson, F Pitel, M Tixier-Boichard, K Ladjali-Mohammedi, J Hillel, A Mäki-Tanila, H H Cheng, M E Delany, J Burnside, S Mizuno

引用次数: 363

Assignment of fibroblast growth factor 18 (FGF18) to human chromosome 5q34 by use of radiation hybrid mapping and fluorescence in situ hybridization. 利用辐射杂交定位和荧光原位杂交技术将成纤维细胞生长因子18 (FGF18)定位到人5q34染色体上。

Cytogenetics and cell genetics Pub Date : 2000-01-01 DOI: 10.1159/000056775

T E Whitmore, M F Maurer, S Sexson, F Raymond, D Conklin, T A Deisher

引用次数: 10

HP1gamma associates with euchromatin and heterochromatin in mammalian nuclei and chromosomes. hp1γ与哺乳动物细胞核和染色体中的常染色质和异染色质有关。

Cytogenetics and cell genetics Pub Date : 2000-01-01 DOI: 10.1159/000056789

E Minc, J C Courvalin, B Buendia

{"title":"HP1gamma associates with euchromatin and heterochromatin in mammalian nuclei and chromosomes.","authors":"E Minc, J C Courvalin, B Buendia","doi":"10.1159/000056789","DOIUrl":"https://doi.org/10.1159/000056789","url":null,"abstract":"<p><p>Heterochromatin protein 1 (HP1) is a nonhistone chromosomal protein, first identified in Drosophila, that plays a dose-dependent role in gene silencing. Three orthologs, HP1alpha, HP1beta, and HP1gamma, have been characterized in mammals. While HP1alpha and HP1beta have been unambiguously localized in heterochromatin by immunocytochemical methods, HP1gamma has been found either exclusively associated with euchromatin or present in both euchromatin and heterochromatin. Here, using an antibody directed against a peptide epitope at the carboxyl-terminal end of the molecule, we localize HP1gamma in both euchromatin and heterochromatin compartments of interphase nuclei, as well as in the pericentromeric chromatin and arms of mitotic chromosomes of 3T3 cells. This dual location was also observed in nuclei expressing HP1gamma as a fusion protein with green fluorescent protein. In contrast, when the distribution of HP1gamma was analyzed with antibodies directed against an amino-terminal epitope, the protein was detectable in euchromatin and not in heterochromatin, except for transient heterochromatin staining during the late S phase, when the heterochromatin undergoes replication. These data suggest that the controversial immunolocalization of HP1gamma in chromatin is due to the use of antibodies directed against topologically distinct epitopes, those present at the amino-terminal end of the molecule being selectively masked in nonreplicative heterochromatin.</p>","PeriodicalId":10982,"journal":{"name":"Cytogenetics and cell genetics","volume":"90 3-4","pages":"279-84"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000056789","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21947039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 174

Assignment of CDK5R2 coding for the cyclin-dependent kinase 5, regulatory subunit 2 (NCK5AI protein) to human chromosome band 2q35 by fluorescent in situ hybridization. CDK5R2编码周期蛋白依赖性激酶5，调控亚基2 (NCK5AI蛋白)通过荧光原位杂交定位到人类染色体2q35带。

Cytogenetics and cell genetics Pub Date : 2000-01-01 DOI: 10.1159/000015602

O V Muravenko, R Z Gizatullin, A I Protopopov, V I Kashuba, E R Zabarovsky, A V Zelenin

{"title":"Assignment of CDK5R2 coding for the cyclin-dependent kinase 5, regulatory subunit 2 (NCK5AI protein) to human chromosome band 2q35 by fluorescent in situ hybridization.","authors":"O V Muravenko, R Z Gizatullin, A I Protopopov, V I Kashuba, E R Zabarovsky, A V Zelenin","doi":"10.1159/000015602","DOIUrl":"https://doi.org/10.1159/000015602","url":null,"abstract":"Neuronal CDC2-like kinase (OMIM: 116940) is a heterodimer of CDK5 (OMIM: 123831) and p25 (nck5a), a neuronspecific 25-kDa regulatory subunit derived proteolytically from NCK5A, encoded by CDK5R1 (neuronal CDK5 activator or cyclin-dependent kinase 5 regulatory subunit 1 gene) (Lew et al., 1994; Tsai et al., 1994). By screening a human hippocampus library with a bovine Nck5a cDNA, Tang et al. (1995) isolated cDNAs encoding NCK5AI, a distinct NCK5A isoform. They also referred to the protein as p39 (nck5ai) based on its calculated molecular mass of 39 kDa. This isoform showed a high degree of sequence similarity to p35 (NCK5A) with 57% amino acid identity. A 30-kDa truncated form of p39 (nck5ai) activated both recombinant and native CDK5 in vitro, as does p25 (nck5a). Northern blot analysis of rat tissues indicated that both NCK5A and p39 (nck5ai) are expressed exclusively in brain. In situ hybridization to rat brain sections revealed that p39 (nck5ai) mRNA was highly expressed in the CA1 to CA3 zone of the hippocampal formation, an area highly enriched in neurons. There was no expression in the fimbria hippocampi, where glial cells predominate. Tang et al. (1995) concluded that p39 (nck5ai) shares many common characteristics with NCK5A, including CDK5-activating activity and brainand neuron-specific expression. Both proteins show limited sequence homology to cyclins, suggesting that they define a new family of cyclin-dependent kinase-activating proteins. The gene encoding p39 (nck5ai) was called CDK5R2. Partial sequencing of the NotI linking clone NR3-007 (Zabarovsky et al., 1994) revealed that it is 100% identical to CDK5R2 over 378 bp (aa 110–235). We concluded that NR3-007 contains part of CDK5R2 and mapped this gene using the NR3-007 clone.","PeriodicalId":10982,"journal":{"name":"Cytogenetics and cell genetics","volume":"89 3-4","pages":"160-1"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000015602","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21800883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Characterization and genomic localization of the mouse Extl2 gene. 小鼠Extl2基因的鉴定和基因组定位。

Cytogenetics and cell genetics Pub Date : 2000-01-01 DOI: 10.1159/000015609

W Wuyts, W Van Hul

引用次数: 7

Genomic structure and chromosome mapping of human and mouse RAMP genes. 人类和小鼠RAMP基因的基因组结构和染色体定位。

Cytogenetics and cell genetics Pub Date : 2000-01-01 DOI: 10.1159/000015644

C Derst, H Engel, K Grzeschik, J Daut

引用次数: 14

The SRY gene HMG-box in micro- and megabats. SRY基因HMG-box在微兆位和兆位。

Cytogenetics and cell genetics Pub Date : 2000-01-01 DOI: 10.1159/000015479

M Bullejos, A Sánchez, M Burgos, R Jiménez, R Díaz De La Guardia