Cytogenetics and cell genetics最新文献_第5页

Suppressor genes for malignant and anchorage-independent phenotypes located on human chromosome 9 have no dosage effects. 位于人类第9号染色体上的恶性和非锚定表型的抑制基因没有剂量效应。

Cytogenetics and cell genetics Pub Date : 2000-01-01 DOI: 10.1159/000015500

M Q Islam, K Islam

{"title":"Suppressor genes for malignant and anchorage-independent phenotypes located on human chromosome 9 have no dosage effects.","authors":"M Q Islam, K Islam","doi":"10.1159/000015500","DOIUrl":"https://doi.org/10.1159/000015500","url":null,"abstract":"We have previously shown that microcell-mediated transfer of a der(9)t(X;9) human chromosome (HSA), derived from human fibroblast strain GM0705, into the Syrian hamster cell line BHK-191-5C produced only near-tetraploid hybrids, although the recipient cell line contained a 1:1 ratio of near-diploid and near-tetraploid cells. However, the tumorigenicity and the anchorage independence could be suppressed in the near-tetraploid hybrids with one copy of the der(9)t(X;9) chromosome. The introduction of an HSA X chromosome did not suppress either of these phenotypes. We concluded that in addition to two suppressor genes, one for tumorigenicity and another for anchorage independence, HSA 9 might carry a third gene capable of inhibiting cellular growth in vitro, which had dosage effects. In the present study, keeping one copy of the der(9)t(X;9) chromosome, we have increased the hamster background chromosome number beyond hexaploid level by fusing two microcell-generated hybrid cell lines, where both malignant and anchorage-independent phenotypes were suppressed, with the parental malignant BHK-191-5C cell line. Tests with nude mice showed that hybrids containing one copy of the der(9)t(X;9) chromosome against the increased background of chromosomes of malignant parental origin were still suppressed for both phenotypes. These results suggest that the suppressor genes for malignancy and for anchorage independence have no dosage effects, in contrast to the suppressor gene(s) for cellular growth.","PeriodicalId":10982,"journal":{"name":"Cytogenetics and cell genetics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000015500","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21623005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

X;Y translocation revealed by chromosome microdissection and FISH in fertile XY females in the Brazilian rodent Akodon montensis. 染色体显微解剖和FISH在巴西啮齿动物Akodon montensis的可育XY雌性中发现的X;Y易位。

Cytogenetics and cell genetics Pub Date : 2000-01-01 DOI: 10.1159/000015504

V Fagundes, A U Christoff, J Scalzi-Martin, J Hozier, C A Moreira-Filho, Y Yonenaga-Yassuda

{"title":"X;Y translocation revealed by chromosome microdissection and FISH in fertile XY females in the Brazilian rodent Akodon montensis.","authors":"V Fagundes, A U Christoff, J Scalzi-Martin, J Hozier, C A Moreira-Filho, Y Yonenaga-Yassuda","doi":"10.1159/000015504","DOIUrl":"https://doi.org/10.1159/000015504","url":null,"abstract":"In a Brazilian population of the neotropical rodent Akodon montensis we found five sex-reversed XY females. These animals were cytogenetically analyzed by chromosome painting using species-specific DNA probes from the Y chromosome, generated by chromosomal microdissection and subsequent use of the degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR). The results showed a chromosome complement with an apparently normal Y chromosome and an X chromosome carrying a translocation that encompasses a large portion of the Y chromosome (seemingly the entire Y). Ovarian histology suggested that these females are fertile. Amplification of the SRY HMG box sequence by PCR shows that at least one copy of the Sry gene is present in the A. montensis XY females. Based on our findings, we suggest that the breakpoint of the X;Y translocation probably altered an X-linked sex-determining locus (or loci), blocking testicular organogenesis in the XY females. Further studies are necessary to determine the precise location and role of this putative sex-determining chromosomal region. Genetic mechanisms of XY sex reversal in A. montensis populations are discussed.","PeriodicalId":10982,"journal":{"name":"Cytogenetics and cell genetics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000015504","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21623009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Assignment of the kinesin family member 4 genes (KIF4A and KIF4B) to human chromosome bands Xq13.1 and 5q33.1 by in situ hybridization. 通过原位杂交将激酶家族成员4基因(KIF4A和KIF4B)定位到人类染色体Xq13.1和5q33.1条带上。

Cytogenetics and cell genetics Pub Date : 2000-01-01 DOI: 10.1159/000015482

M J Ha, J Yoon, E Moon, Y M Lee, H J Kim, W Kim

引用次数: 18

Cloning and characterization of human FTCD on 21q22.3, a candidate gene for glutamate formiminotransferase deficiency. 谷氨酸甲氨基转移酶缺乏症候选基因21q22.3的克隆与鉴定

Cytogenetics and cell genetics Pub Date : 2000-01-01 DOI: 10.1159/000015483

A Solans, X Estivill, S de la Luna

引用次数: 30

Assignment of the gene GRM1 coding for metabotropic glutamate receptor 1 to human chromosome band 6q24 by in situ hybridization. 代谢性谷氨酸受体1编码基因GRM1在人类染色体6q24带上的原位杂交。

Cytogenetics and cell genetics Pub Date : 2000-01-01 DOI: 10.1159/000015517

S Ganesh, K Amano, K Yamakawa

{"title":"Assignment of the gene GRM1 coding for metabotropic glutamate receptor 1 to human chromosome band 6q24 by in situ hybridization.","authors":"S Ganesh, K Amano, K Yamakawa","doi":"10.1159/000015517","DOIUrl":"https://doi.org/10.1159/000015517","url":null,"abstract":"Metabotropic glutamate receptors (mGluRs) are important modulators of synaptic transmission and have been implicated in epilepsy, neurotoxicity and neurodegenerative disorders (Schoepp and Conn, 1993). Based on amino acid sequence similarity and agonist selectivity, mGluRs are divided into three groups (Pin and Duvoisin, 1995). Subtype mGluR1 classified as group I has been shown to stimulate the phosphoinositide hydrolysis pathway (Schoepp and Conn, 1993). Mice lacking the mGluR1 gene develop ataxic gait and intention tremor (Aiba et al., 1994). Previously, the human gene for mGluR1 (GRM1) was mapped on chromosome 6 by Southern hybridization using human/rodent somatic cell hybrids (Stephan et al., 1996) and radiation hybrid mapping placed this gene between the markers D6S453 and D6S311, about a 4-cM interval on 6q (G3 Map; www.ncbi.nlm.nih.gov/genemap99). Chromosome band 6q24 contains one of the loci for schizoaffective disorder (Kaufmann et al., 1998) and also the locus for Lafora type epilepsy (Sainz et al., 1997). Using a BAC/YAC based physical map constructed for the 6q24 region (Ganesh et al., unpublished) we refined the chromosome position of GRM1. We show that GRM1 is located on chromosome band 6q24, near the genetic marker D6S1480 but distal to EPM2A, a gene recently shown to be involved in Lafora disease (Minassian et al., 1998). Interestingly, GRIK2, the gene for ionotropic glutamate receptor kainate 2, is also located on 6q, at 6q16.3→q21 (Paschen et al., 1994). Materials and methods","PeriodicalId":10982,"journal":{"name":"Cytogenetics and cell genetics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000015517","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21673116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Assignment of mimecan gene (OGN) to human chromosome band 9q22 by in situ hybridization. mimecan基因(OGN)在人染色体9q22带的原位杂交。

Cytogenetics and cell genetics Pub Date : 2000-01-01 DOI: 10.1159/000015521

E S Tasheva, M Pettenati, C Von Kap-Her, G W Conrad

引用次数: 15

Identification and mapping of swine cyclin-dependent kinase inhibitor CDKN2A and CDKN2B exon 2 sequences. 猪周期蛋白依赖性激酶抑制剂CDKN2A和CDKN2B外显子2序列的鉴定和定位。

Cytogenetics and cell genetics Pub Date : 2000-01-01 DOI: 10.1159/000015527

C Le Chalony, H Hayes, G Frelat, C Geffrotin

引用次数: 5

Chromosome mapping of the human genes encoding the MAP kinase kinase MEK1 (MAP2K1) to 15q21 and MEK2 (MAP2K2) to 7q32. 人类基因编码MAP激酶MEK1 (MAP2K1)至15q21和MEK2 (MAP2K2)至7q32的染色体定位。

Cytogenetics and cell genetics Pub Date : 2000-01-01 DOI: 10.1159/000015530

S Meloche, K Gopalbhai, B G Beatty, S W Scherer, J Pellerin

引用次数: 7

Assignment of E-cadherin (CDH1) and KSP-cadherin (CDH16) to chromosome 16q22.1 by radiation hybrid mapping. E-cadherin (CDH1)和KSP-cadherin (CDH16)在16q22.1染色体上的定位

Cytogenetics and cell genetics Pub Date : 2000-01-01 DOI: 10.1159/000015531

D Baudry, C Jeanpierre

{"title":"Assignment of E-cadherin (CDH1) and KSP-cadherin (CDH16) to chromosome 16q22.1 by radiation hybrid mapping.","authors":"D Baudry, C Jeanpierre","doi":"10.1159/000015531","DOIUrl":"https://doi.org/10.1159/000015531","url":null,"abstract":"Cadherins are cellular adhesion molecules. Since disturbance of intracellular adhesion is important for invasion and metastasis of tumor cells, cadherins are considered prime candidates for tumor suppressor genes. A variety of solid tumors show loss of heterozygosity for the long arm of chromosome 16 (Austruy et al., 1995; Driouch et al., 1997), which is indicative of the potential localization of tumor suppressor genes. The homophilic cell adhesion molecule E-cadherin (CDH1) has been involved in gastric (Becker et al., 1994), breast (Berx et al., 1995) and gynecologic carcinomas (Risinger et al., 1994). This report refined localization of: (1) E-cadherin (CDH1), previously mapped to 16q22.1 on a panel of somatic cell hybrids (Callen et al., 1995) and between WI-9392 and D16S496 on the Genebridge 4 radiation hybrid panel (Hunstman et al., 1998); (2) KSP-cadherin (CDH16), previously mapped to chromosome 16q21-proximal 16q22 by in situ hybridization (Thomson et al., 1998). A more precise localization of these two genes in a publicly available radiation hybrid map will facilitate marker selection for linkage and loss of heterozygosity analyses. Materials and methods","PeriodicalId":10982,"journal":{"name":"Cytogenetics and cell genetics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000015531","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21673211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Identification and characterization of mouse orthologs of the AMMECR1 and FACL4 genes deleted in AMME syndrome: orthology of Xq22.3 and MmuXF1-F3. AMME综合征中缺失的AMMECR1和FACL4基因小鼠同源基因的鉴定和表征:Xq22.3和MmuXF1-F3的同源基因。

Cytogenetics and cell genetics Pub Date : 2000-01-01 DOI: 10.1159/000015533

F Vitelli, I Meloni, S Fineschi, F Favara, C Tiziana Storlazzi, M Rocchi, A Renieri

引用次数: 12