Molecular cloning and characterization of a mouse homolog of human TNFSF14, a member of the TNF superfamily.

K Misawa, T Nosaka, T Kojima, M Hirai, T Kitamura
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引用次数: 15

Abstract

A member of the tumor necrosis factor (TNF) superfamily, human TNFSF14 (hTNFSF14)/HVEM-L (herpes virus entry mediator ligand) was isolated as a cellular ligand for HVEM/TR2 and human lymphotoxin beta receptor (LTbetaR). TNFSF14 induces apoptosis and suppresses tumor formation. We have isolated a cDNA clone for a mouse homologue of hTNFSF14 by signal sequence trap (SST) screening which we recently developed. The deduced amino acid sequence of the mouse TNFSF14 (mTNFSF14) cDNA comprised 239 amino acid residues and was 77% identical to the hTNFSF14 protein. In Northern blot analysis, 2.1 kb and 4.2kb mTNFSF14 transcripts were detected in spleen and lung, and in heart, respectively. Fluorescence in situ hybridization analysis localized the mTNFSF14 gene Tnfsf14 to chromosome 17 which is tightly linked with Tnf, Lta, and Ltb.

人类TNF超家族成员TNFSF14小鼠同源物的分子克隆和表征。
作为肿瘤坏死因子(TNF)超家族的成员,人TNFSF14 (hTNFSF14)/HVEM- l(疱疹病毒进入介质配体)作为HVEM/TR2和人淋巴毒素β受体(LTbetaR)的细胞配体被分离出来。TNFSF14诱导细胞凋亡,抑制肿瘤形成。通过信号序列陷阱(SST)筛选,我们分离出了hTNFSF14小鼠同源基因的cDNA克隆。小鼠TNFSF14 (mTNFSF14) cDNA的氨基酸序列包含239个氨基酸残基,与hTNFSF14蛋白的同源性为77%。在Northern blot分析中,在脾脏和肺以及心脏中分别检测到2.1 kb和4.2kb的mTNFSF14转录本。荧光原位杂交分析将mTNFSF14基因定位在17号染色体上,该基因与Tnf、Lta和Ltb紧密相连。
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