Cytogenetics and cell genetics最新文献_第7页

Molecular cloning and characterization of a mouse homolog of human TNFSF14, a member of the TNF superfamily. 人类TNF超家族成员TNFSF14小鼠同源物的分子克隆和表征。

Cytogenetics and cell genetics Pub Date : 2000-01-01 DOI: 10.1159/000015570

K Misawa, T Nosaka, T Kojima, M Hirai, T Kitamura

引用次数: 15

Assignment of the human RhoHP1 gene (ARHD) to chromosome 11q14.3 by radiation hybrid mapping. 人类RhoHP1基因(ARHD)在染色体11q14.3上的定位

Cytogenetics and cell genetics Pub Date : 2000-01-01 DOI: 10.1159/000015562

H S Kim, J Y Choi, A R Jung, K L Jang, W H Lee, W C Choi, T J Crow, B H Hyun

引用次数: 0

First report on chicken genes and chromosomes 2000. 鸡基因和染色体首次报道2000。

Cytogenetics and cell genetics Pub Date : 2000-01-01 DOI: 10.1159/000056772

M Schmid, I Nanda, M Guttenbach, C Steinlein, M Hoehn, M Schartl, T Haaf, S Weigend, R Fries, J M Buerstedde, K Wimmers, D W Burt, J Smith, S A'Hara, A Law, D K Griffin, N Bumstead, J Kaufman, P A Thomson, T Burke, M A Groenen, R P Crooijmans, A Vignal, V Fillon, M Morisson, F Pitel, M Tixier-Boichard, K Ladjali-Mohammedi, J Hillel, A Mäki-Tanila, H H Cheng, M E Delany, J Burnside, S Mizuno

引用次数: 363

Assignment of fibroblast growth factor 18 (FGF18) to human chromosome 5q34 by use of radiation hybrid mapping and fluorescence in situ hybridization. 利用辐射杂交定位和荧光原位杂交技术将成纤维细胞生长因子18 (FGF18)定位到人5q34染色体上。

Cytogenetics and cell genetics Pub Date : 2000-01-01 DOI: 10.1159/000056775

T E Whitmore, M F Maurer, S Sexson, F Raymond, D Conklin, T A Deisher

引用次数: 10

Assignment of the 100-kDa subunit of cleavage and polyadenylation specificity factor (CPSF2) to human chromosome 14q31.3 by radiation hybrid mapping. 通过辐射杂交定位将切割和多腺苷化特异性因子(CPSF2)的100 kda亚基定位到人类染色体14q31.3。

Cytogenetics and cell genetics Pub Date : 2000-01-01 DOI: 10.1159/000056798

M Samiotaki, N A Balatsos, N Courtis, C M Tsiapalis

{"title":"Assignment of the 100-kDa subunit of cleavage and polyadenylation specificity factor (CPSF2) to human chromosome 14q31.3 by radiation hybrid mapping.","authors":"M Samiotaki, N A Balatsos, N Courtis, C M Tsiapalis","doi":"10.1159/000056798","DOIUrl":"https://doi.org/10.1159/000056798","url":null,"abstract":"The generation of a new 3) end is part of the eukaryotic premRNA maturation process, in which a poly(A) tail is added by two coupled reactions: endonucleolytic cleavage at the poly(A) site followed by the polyadenylation of the upstream cleaved product (Wahle and Kuhn, 1997). A multicomponent complex sufficient to complete the 3) processing reactions is comprised of Cleavage and Polyadenylation Specificity Factor (CPSF), CstF (Cleavage stimulation Factor), CF Im (Cleavage Factor I), CF IIm (Cleavage Factor II), PAP (Poly(A) polymerase) and PABP2 (PolyA Binding Protein 2). Most of the proteins involved in this finely orchestrated process have been purified and extensively studied (Wahle and Rüegsegger, 1999). CPSF consists of four subunits of 160, 100, 73 and 30 kDa. The exact function of the 100-kDa CPSF subunit is still unknown, while its presence is essential for the maturation process (Jenny et al., 1994). The full length of the human CPSF 100-kDa subunit cDNA was cloned by Nagase et al. (2000) (GenBank accession number AB037788.1), while its bovine homologue was cloned by Jenny et al. (1994) (GenBank accession number X75931).","PeriodicalId":10982,"journal":{"name":"Cytogenetics and cell genetics","volume":"90 3-4","pages":"328-9"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000056798","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21946445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

HP1gamma associates with euchromatin and heterochromatin in mammalian nuclei and chromosomes. hp1γ与哺乳动物细胞核和染色体中的常染色质和异染色质有关。

Cytogenetics and cell genetics Pub Date : 2000-01-01 DOI: 10.1159/000056789

E Minc, J C Courvalin, B Buendia

{"title":"HP1gamma associates with euchromatin and heterochromatin in mammalian nuclei and chromosomes.","authors":"E Minc, J C Courvalin, B Buendia","doi":"10.1159/000056789","DOIUrl":"https://doi.org/10.1159/000056789","url":null,"abstract":"<p><p>Heterochromatin protein 1 (HP1) is a nonhistone chromosomal protein, first identified in Drosophila, that plays a dose-dependent role in gene silencing. Three orthologs, HP1alpha, HP1beta, and HP1gamma, have been characterized in mammals. While HP1alpha and HP1beta have been unambiguously localized in heterochromatin by immunocytochemical methods, HP1gamma has been found either exclusively associated with euchromatin or present in both euchromatin and heterochromatin. Here, using an antibody directed against a peptide epitope at the carboxyl-terminal end of the molecule, we localize HP1gamma in both euchromatin and heterochromatin compartments of interphase nuclei, as well as in the pericentromeric chromatin and arms of mitotic chromosomes of 3T3 cells. This dual location was also observed in nuclei expressing HP1gamma as a fusion protein with green fluorescent protein. In contrast, when the distribution of HP1gamma was analyzed with antibodies directed against an amino-terminal epitope, the protein was detectable in euchromatin and not in heterochromatin, except for transient heterochromatin staining during the late S phase, when the heterochromatin undergoes replication. These data suggest that the controversial immunolocalization of HP1gamma in chromatin is due to the use of antibodies directed against topologically distinct epitopes, those present at the amino-terminal end of the molecule being selectively masked in nonreplicative heterochromatin.</p>","PeriodicalId":10982,"journal":{"name":"Cytogenetics and cell genetics","volume":"90 3-4","pages":"279-84"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000056789","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21947039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 174

Identification and characterization of mouse orthologs of the AMMECR1 and FACL4 genes deleted in AMME syndrome: orthology of Xq22.3 and MmuXF1-F3. AMME综合征中缺失的AMMECR1和FACL4基因小鼠同源基因的鉴定和表征：Xq22.3和MmuXF1-F3的同源基因。

Cytogenetics and cell genetics Pub Date : 2000-01-01 DOI: 10.1159/000015533

F Vitelli, I Meloni, S Fineschi, F Favara, C Tiziana Storlazzi, M Rocchi, A Renieri