Cryo letters最新文献_第9页

In vitro cold acclimation is required for successful cryopreservation of Juglans regia L. shoot tips. 体外冷驯化是成功低温保存核桃茎尖的必要条件。

IF 1 4区生物学

Cryo letters Pub Date : 2023-07-01 DOI: 10.54680/fr23410110612

S. Kushnarenko, Nazgul K. Rymkhanova, Moldir Aralbayeva, N. Romadanova

{"title":"In vitro cold acclimation is required for successful cryopreservation of Juglans regia L. shoot tips.","authors":"S. Kushnarenko, Nazgul K. Rymkhanova, Moldir Aralbayeva, N. Romadanova","doi":"10.54680/fr23410110612","DOIUrl":"https://doi.org/10.54680/fr23410110612","url":null,"abstract":"BACKGROUND\u0000Persian walnut (Juglans regia L.) is one of the most economically important nut crops. In the Western Tien Shan in Kazakhstan, walnut forests are the northernmost in the natural range of this species. Shoot tip cryopreservation is an important strategy to ensure long-term clonal conservation of plant genetic resources.\u0000\u0000\u0000OBJECTIVE\u0000To determine the effect of cold acclimation duration (0-6 weeks) with alternating temperatures (8 h at 22 degree C, light intensity 10 μmol m-2 s-1/16 h at -1 degree C, in the dark) and of plant vitrification solution 2 (PVS2) exposure duration (30, 50, 80 or 100 min) on shoot tip regrowth after cryopreservation by vitrification.\u0000\u0000\u0000MATERIALS AND METHODS\u0000In vitro-grown shoots of three wild Juglans regia accessions from a native population of Sairam-Ugam National Natural Park in the south of Kazakhstan and of cultivar Milotai 10 were used as sources of plant material. Shoot tips (1.8-2.0 mm with five to six leaf primordia) were excised from in vitro-grown shoots and cryopreserved using PVS2 vitrification technique.\u0000\u0000\u0000RESULTS\u0000Regrowth of cryopreserved shoot tips increased and was significantly higher (P < 0.05) after 4-6 weeks cold acclimation with the highest regrowth between 59.9-67.8 degree after 5 weeks for the four genotypes tested. The highest regrowth level was obtained after 80 min of PVS2 exposure, which was significantly better (P < 0.05) compared to 30, 50 or 100 min exposure.\u0000\u0000\u0000CONCLUSION\u0000The PVS2 vitrification protocol established is very effective for cryopreservation of both unique wild J. regia germplasm and of walnut cultivars. Doi: 10.54680/fr23410110612.","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":"159 1","pages":"240-248"},"PeriodicalIF":1.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76970132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative cryopreservation of indian wild orange (Citrus indica Tanaka) embryonic axes. 印度野生柑桔(Citrus indica Tanaka)胚轴的比较冷冻保存。

IF 1 4区生物学

Cryo letters Pub Date : 2023-05-01 DOI: 10.54680/fr23310110512

S. K. Malik, S. Kaur, R. Choudhary, R. Chaudhury, H. Pritchard

{"title":"Comparative cryopreservation of indian wild orange (Citrus indica Tanaka) embryonic axes.","authors":"S. K. Malik, S. Kaur, R. Choudhary, R. Chaudhury, H. Pritchard","doi":"10.54680/fr23310110512","DOIUrl":"https://doi.org/10.54680/fr23310110512","url":null,"abstract":"BACKGROUND\u0000Indian Wild Orange (Citrus indica Tanaka) is an endangered and endemic species from northeast India for which effective ex situ conservation strategies, including embryo cryopreservation, are urgently needed.\u0000\u0000\u0000MATERIALS AND METHODS\u0000Desiccation tolerance and cryopreservation ability for embryonic axes of Citrus indica was determined using three techniques (air desiccation-freezing, PVS2 vitrification-freezing and encapsulation-dehydration-freezing). Success was assessed as survival and recovery in vitro.\u0000\u0000\u0000RESULTS\u0000Successful cryopreservation of embryonic axes was achieved using all three methods, with the highest survival achieved when using air desiccation-freezing (90%) followed by encapsulation-dehydration (85%) and PVS2 vitrification cryopreservation (80%). Regeneration levels were lower than survival levels for all three proceedures. Post-cryo regeneration success was: encapsulation-dehydration (64%) > air desiccation-freezing (55%) > PVS2 vitrification (52%).\u0000\u0000\u0000CONCLUSION\u0000Although there was relatively high post-cryopreservation recovery growth obtained using all the three techniques, the air desiccation-freezing technique is preferred, as it is a simple, practical and reproducible technique for the long-term cryobanking of this important wild species. Doi: 10.54680/fr23310110512.","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":"24 1","pages":"142-150"},"PeriodicalIF":1.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78980826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aqueous extract OF Moringa oleifera Lam leaves added to freezing extenders damages goat sperm membranes. 辣木叶水提物加入冷冻剂中会破坏山羊精子膜。

IF 1 4区生物学

Cryo letters Pub Date : 2023-05-01 DOI: 10.54680/fr23310110712

Desirée Coelho de Mello Seal, M. M. Monteiro, L. Arruda, Jerônimo Hugo de Souza, Robson Raion de Vasconcelos Alves, Luiz Alberto Lira Soares, T. Napoleão, P. M. Guedes Paiva, Lucas Eduardo Bezerra de Lima, Regina C. Bressan Queiroz de Figueiredo, M. Guerra

{"title":"Aqueous extract OF Moringa oleifera Lam leaves added to freezing extenders damages goat sperm membranes.","authors":"Desirée Coelho de Mello Seal, M. M. Monteiro, L. Arruda, Jerônimo Hugo de Souza, Robson Raion de Vasconcelos Alves, Luiz Alberto Lira Soares, T. Napoleão, P. M. Guedes Paiva, Lucas Eduardo Bezerra de Lima, Regina C. Bressan Queiroz de Figueiredo, M. Guerra","doi":"10.54680/fr23310110712","DOIUrl":"https://doi.org/10.54680/fr23310110712","url":null,"abstract":"BACKGROUND\u0000Semen cryopreservation is a biotechnology used frequently in animal production; however, there are some obstacles, such as those caused by high levels of reactive oxygen species (ROS). Moringa oleifera (MO) is known as a potent source of antioxidants and might be an important adjuvant.\u0000\u0000\u0000OBJECTIVE\u0000The objective of this study was to determine the effect of different concentrations of MO extract supplementation on goat semen cryopreservation efficiency.\u0000\u0000\u0000MATERIALS AND METHODS\u0000Ejaculates (n=6) from four goat breeders were pooled and diluted in skimmed milk (SM) or Tris-egg yolk (TEY)-based extenders and supplemented with different concentrations of MO extract (0, 1, 2 and 5 mg/mL). After the freeze-thaw cycle, sperm kinetics and viability were assessed.\u0000\u0000\u0000RESULTS\u0000With the SM extender, straightness, wobble and plasma membrane integrity were lower than in the control group (P < 0.05). With the TEY extender, wobble was lower in with 5 mg/mL MO extract than in the control group (P < 0.05). As regards sperm ultrastructure, evaluated by SEM, the MO extract, regardless of the diluent used, damaged the membrane of sperm cells in a dose-dependent manner.\u0000\u0000\u0000CONCLUSION\u0000The addition of aqueous extract of MO leaves in both diluents at all concentrations tested affects the parameters of sperm progressivity and damages the plasma membrane in a dose-dependent manner. DOI: 10.54680/fr23310110712.","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":"1 1","pages":"151-159"},"PeriodicalIF":1.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86558628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Motility, viability and fertility of goldfish Carassius auratus (Pisces: Cyprinidae) post short-term cryopreservation. 金鱼短期冷冻保存后的活力、活力和繁殖能力。

IF 1 4区生物学

Cryo letters Pub Date : 2023-05-01

N Nurlaili, K Eriani, I Salma, S Maulida, S R Rahayu, L S Handayani, F K Kocabas, M N Siti-Azizah, M Wilkes, Z A Muchlisin

{"title":"Motility, viability and fertility of goldfish Carassius auratus (Pisces: Cyprinidae) post short-term cryopreservation.","authors":"N Nurlaili, K Eriani, I Salma, S Maulida, S R Rahayu, L S Handayani, F K Kocabas, M N Siti-Azizah, M Wilkes, Z A Muchlisin","doi":"","DOIUrl":"","url":null,"abstract":"Backgrund: Goldfish Carassius auratus is a popular ornamental fish extensively cultured worldwide. Sperm cryopreservation is a common fish breeding method that ensures sperm availability around the year. Studies on cryopreservation of goldfish sperm, especially on the suitability of cryoprotectant types and pre-freezing time, are scarcely available.Objective: To determine the most suitable type of cryoprotectant and pre-freezing for the successful cryopreservation of goldfish sperm.Materials and methods: A completely randomized design with two factors was utilized in this study. The first factor is the type of cryoprotectants, which included methanol, ethanol, ethylene glycol, glycerol, and DMSO. The second is pre-freezing times of 10, 20, 30, and 40 min at each of the pre-freezing temperatures of 4 degree C, -10 degree C, and -79 degree C, meaning that the total times for the ramping down of temperature were 30, 60, 90 and 120 min, respectively. The Ringer solution and 10% egg yolk were used as extender and extracellular cryoprotectant. The sperm was stored at -179 degree C for 7 days.Results: The ANOVA test showed that cryoprotectants and pre-freezing significantly affected the motility, viability, and fertility of goldfish sperm after freezing in liquid nitrogen for 7 days (P<0.05). Furthermore, 10% DMSO combined with 15% egg yolk with an pre-freezing time of 20 min can maintain sperm motility, viability, and fertility higher than other treatments, by 79%, 80%, and 33%, respectively. The agarose gel electrophoresis showed no DNA fragmentation in all samples, including fresh sperm.Conclusion: We conclude that 10% DMSO combined with 15% egg yolk and 20 min pre-freezing is the best treatment for goldfish sperm cryopreservation. DOI: 10.54680/fr23310110412.","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":"44 3","pages":"169-177"},"PeriodicalIF":1.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50160975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative cryopreservation of indian wild orange (Citrus indica Tanaka) embryonic axes. 印度野橙（Citrus indica Tanaka）胚胎轴的比较冷冻保存。

IF 1 4区生物学

Cryo letters Pub Date : 2023-05-01

S K Malik, S Kaur, R Choudhary, R Chaudhury, H W Pritchard

{"title":"Comparative cryopreservation of indian wild orange (Citrus indica Tanaka) embryonic axes.","authors":"S K Malik, S Kaur, R Choudhary, R Chaudhury, H W Pritchard","doi":"","DOIUrl":"","url":null,"abstract":"Background: Indian Wild Orange (Citrus indica Tanaka) is an endangered and endemic species from northeast India for which effective ex situ conservation strategies, including embryo cryopreservation, are urgently needed.Materials and methods: Desiccation tolerance and cryopreservation ability for embryonic axes of Citrus indica was determined using three techniques (air desiccation-freezing, PVS2 vitrification-freezing and encapsulation-dehydration-freezing). Success was assessed as survival and recovery in vitro.Results: Successful cryopreservation of embryonic axes was achieved using all three methods, with the highest survival achieved when using air desiccation-freezing (90%) followed by encapsulation-dehydration (85%) and PVS2 vitrification cryopreservation (80%). Regeneration levels were lower than survival levels for all three proceedures. Post-cryo regeneration success was: encapsulation-dehydration (64%) > air desiccation-freezing (55%) > PVS2 vitrification (52%).Conclusion: Although there was relatively high post-cryopreservation recovery growth obtained using all the three techniques, the air desiccation-freezing technique is preferred, as it is a simple, practical and reproducible technique for the long-term cryobanking of this important wild species. Doi: 10.54680/fr23310110512.","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":"44 3","pages":"142-150"},"PeriodicalIF":1.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50161062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The safety of human embryos following long-term cryopreservation ( >6 years) on vitrification. 玻璃化冷冻保存（>6年）后人类胚胎的安全性。

IF 1 4区生物学

Cryo letters Pub Date : 2023-05-01

H He, R Jiang, X Ren, L Jin, Y Jiang

{"title":"The safety of human embryos following long-term cryopreservation ( >6 years) on vitrification.","authors":"H He, R Jiang, X Ren, L Jin, Y Jiang","doi":"","DOIUrl":"","url":null,"abstract":"Background: Vitrification of embryos has become the basic means of assisted reproductive technology (ART) therapy in recent years. Concerns have also been raised about the safety of vitrification and the effect of cryopreservation time. Most of the previous studies were on the data within 6 years of cryopreservation.Objective: In this study, we aimed to evaluate the impact of long-term cryopreservation (>6 years) on pregnancy and neonatal outcomes.Materials and methods: This research was a single-center, retrospective analysis, including 426 frozen-thawed embryo transfer (FET) cycles. Patients who participated in IVF-FET cycles between January 2013 to December 2020 were analyzed. Preferentially matched participants were divided into three groups according to storage time: group A (>72 months), group B (0-3 months, propensity score matching [PSM] according to the age of oocyte retrieval), and group C (0-3 months, PSM according to the age of embryo transfer).Results: Our results revealed that there were no significant differences in human chorionic gonadotropin [HCG] positive rate, clinical pregnancy rate, miscarriage rate, live birth rate, and neonatal outcomes when the embryo storage duration >72 months. But the proportion of high birth weight was higher in group A (>72 months) when matched according to age at embryo transfer.Conclusion: The results of our study showed that long-term cryopreservation had no effect on the pregnancy and neonatal outcomes of vitrification. The results offer evidence for the safety of using long-term cryopreservation embryos after vitrification. DOI: 10.54680/fr23310110612.","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":"44 3","pages":"178-184"},"PeriodicalIF":1.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50160977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessing the benefits of antioxidant and antibiotics treatments for cryopreservation of the model alga Ectocarpus siliculosus and the endemic alga Acinetospora asiatica. 抗氧化和抗生素处理对模式藻硅酸外露和特有藻亚洲动孢的低温保存效果评估。

IF 1 4区生物学

Cryo letters Pub Date : 2023-05-01 DOI: 10.54680/fr23310110212

José Avila-Peltroche, Boo Yeon Won, Tae Oh Cho

{"title":"Assessing the benefits of antioxidant and antibiotics treatments for cryopreservation of the model alga Ectocarpus siliculosus and the endemic alga Acinetospora asiatica.","authors":"José Avila-Peltroche, Boo Yeon Won, Tae Oh Cho","doi":"10.54680/fr23310110212","DOIUrl":"https://doi.org/10.54680/fr23310110212","url":null,"abstract":"BACKGROUND\u0000Cryopreservation in liquid nitrogen is a suitable technique for preserving seaweeds, a group of photosynthetic organisms with many applications. Although there are some standard protocols for seaweed cryopreservation, most rely on expensive controlled-rate coolers. Moreover, several factors, such as the use of antioxidants or antibiotics, remain unexplored.\u0000\u0000\u0000OBJECTIVE\u0000To test the effect of 2-mercapthoethanol (antioxidant) and antibiotic mixtures on the cryopreservation of the model alga Ectocarpus siliculosus and the endemic brown seaweed Acinetospora asiatica using a low-tech passive rate cooler.\u0000\u0000\u0000MATERIALS AND METHODS\u00002-mercaptoethanol was added to the cryoprotectant (CPA) solution, while antibiotic mixtures were included in the culture medium during the recovery process. In addition, two CPA solutions were tested on E. siliculosus.\u0000\u0000\u0000RESULTS\u0000After two weeks of recovery, the treatment comprising PSC antibiotic mixture (Penicillin G, streptomycin, and chloramphenicol) showed a significant increase in post-thaw viability. Antioxidant treatment did not improve viability. The highest viabilities for E. siliculosus and A. asiatica were 64-83%, and 83-87%, respectively, using 10% glycerol + 10% proline as CPA solution.\u0000\u0000\u0000CONCLUSION\u0000E. siliculosus and A. asiatica were successfully cryopreserved using a low-tech passive rate cooler, 10% glycerol + 10% proline solution, and antibiotic treatment. The highest post-thaw viabilities (64-87%) reported for PSC antibiotic mixture suggest the potential benefits of using antibiotics during post-thaw recovery of marine macroalgae. This study is the first report on the cryopreservation of A. asiatica. DOI: 10.54680/fr23310110212.","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":"19 1","pages":"160-168"},"PeriodicalIF":1.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82522047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The safety of human embryos following long-term cryopreservation ( >6 years) on vitrification. 玻璃化长期冷冻保存(>6年)后人类胚胎的安全性。

IF 1 4区生物学

Cryo letters Pub Date : 2023-05-01 DOI: 10.54680/fr23310110612

H. He, Rui Jiang, X. Ren, L. Jin, Yaping Jiang

{"title":"The safety of human embryos following long-term cryopreservation ( >6 years) on vitrification.","authors":"H. He, Rui Jiang, X. Ren, L. Jin, Yaping Jiang","doi":"10.54680/fr23310110612","DOIUrl":"https://doi.org/10.54680/fr23310110612","url":null,"abstract":"BACKGROUND\u0000Vitrification of embryos has become the basic means of assisted reproductive technology (ART) therapy in recent years. Concerns have also been raised about the safety of vitrification and the effect of cryopreservation time. Most of the previous studies were on the data within 6 years of cryopreservation.\u0000\u0000\u0000OBJECTIVE\u0000In this study, we aimed to evaluate the impact of long-term cryopreservation (>6 years) on pregnancy and neonatal outcomes.\u0000\u0000\u0000MATERIALS AND METHODS\u0000This research was a single-center, retrospective analysis, including 426 frozen-thawed embryo transfer (FET) cycles. Patients who participated in IVF-FET cycles between January 2013 to December 2020 were analyzed. Preferentially matched participants were divided into three groups according to storage time: group A (>72 months), group B (0-3 months, propensity score matching [PSM] according to the age of oocyte retrieval), and group C (0-3 months, PSM according to the age of embryo transfer).\u0000\u0000\u0000RESULTS\u0000Our results revealed that there were no significant differences in human chorionic gonadotropin [HCG] positive rate, clinical pregnancy rate, miscarriage rate, live birth rate, and neonatal outcomes when the embryo storage duration >72 months. But the proportion of high birth weight was higher in group A (>72 months) when matched according to age at embryo transfer.\u0000\u0000\u0000CONCLUSION\u0000The results of our study showed that long-term cryopreservation had no effect on the pregnancy and neonatal outcomes of vitrification. The results offer evidence for the safety of using long-term cryopreservation embryos after vitrification. DOI: 10.54680/fr23310110612.","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":"10 1","pages":"178-184"},"PeriodicalIF":1.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76679147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Cryoprotectant and Pre-Freezing on the Sperm Motility, Viability and Fertility of Goldfish Carassius Auratus (Pisces: Cyprinidae) Post Short-Term Cryopreservation 冷冻保护剂和预冷冻对鲫鱼(Carassius Auratus)短期冷冻后精子活力、活力和生育能力的影响

IF 1 4区生物学

Cryo letters Pub Date : 2023-05-01 DOI: 10.54680/fr23310110412

N. Nurlaili, K. Eriani, I. Salma, S. Maulida, Sri Riska Rahayu, Luvi Syafrida Handayani, F. Kocabaş, M. N. Siti-Azizah, M. Wilkes, Z. Muchlisin

{"title":"Effect of Cryoprotectant and Pre-Freezing on the Sperm Motility, Viability and Fertility of Goldfish Carassius Auratus (Pisces: Cyprinidae) Post Short-Term Cryopreservation","authors":"N. Nurlaili, K. Eriani, I. Salma, S. Maulida, Sri Riska Rahayu, Luvi Syafrida Handayani, F. Kocabaş, M. N. Siti-Azizah, M. Wilkes, Z. Muchlisin","doi":"10.54680/fr23310110412","DOIUrl":"https://doi.org/10.54680/fr23310110412","url":null,"abstract":"BACKGRUND: Goldfish Carassius auratus is a popular ornamental fish extensively cultured worldwide. Sperm cryopreservation is a common fish breeding method that ensures sperm availability around the year. Studies on cryopreservation of goldfish sperm, especially on the\u0000 suitability of cryoprotectant types and pre-freezing time, are scarcely available. OBJECTIVE: To determine the most suitable type of cryoprotectant and pre-freezing for the successful cryopreservation of goldfish sperm. MATERIALS AND METHODS: A completely randomized design with\u0000 two factors was utilized in this study. The first factor is the type of cryoprotectants, which included methanol, ethanol, ethylene glycol, glycerol, and DMSO. The second is pre-freezing times of 10, 20, 30, and 40 min at each of the pre- freezing temperatures of 4°C, −10°C,\u0000 and −79°C, meaning that the total times for the ramping down of temperature were 30, 60, 90 and 120 min, respectively. The Ringer solution and 10% egg yolk were used as extender and extracellular cryoprotectant. The sperm was stored at −179°C for 7 days. RESULTS:\u0000 The ANOVA test showed that cryoprotectants and pre-freezing significantly affected the motility, viability, and fertility of goldfish sperm after freezing in liquid nitrogen for 7 days (ρ<0.05). Furthermore, 10% DMSO combined with 15% egg yolk with an pre-freezing time\u0000 of 20 min can maintain sperm motility, viability, and fertility higher than other treatments, by 79%, 80%, and 33%, respectively. The agarose gel electrophoresis showed no DNA fragmentation in all samples, including fresh sperm. CONCLUSION: We conclude that 10%\u0000 DMSO combined with 15% egg yolk and 20 min pre-freezing is the best treatment for goldfish sperm cryopreservation.","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":"77 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74828390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of isolation protocols and cryoprotectants on freezing of stallion epididymal spermatozoa. 分离方案和冷冻保护剂对种马附睾精子冷冻的影响。

IF 1 4区生物学

Cryo letters Pub Date : 2023-05-01

T R Talluri, D Jhamb, N Paul, J Singh, R K Dedar, S C Mehta, R A Legha, Y Pal

{"title":"Effect of isolation protocols and cryoprotectants on freezing of stallion epididymal spermatozoa.","authors":"T R Talluri, D Jhamb, N Paul, J Singh, R K Dedar, S C Mehta, R A Legha, Y Pal","doi":"","DOIUrl":"","url":null,"abstract":"Background: The recovery of spermatozoa from the cauda epididymis may be the only option to obtain genetic material from elite stallions that had undergone castration or sudden death due to colic or severe injury.Objective: To evaluate two different protocols for retrieval of stallion epididymal spermatozoa and to evaluate different cryoprotectants on the freezability of the epididymal spermatozoa.Materials and methods: Six epididymides from three stallions were collected immediately after routine castration under general anesthesia. In the first experiment, each epididymis (of two testes) of the same stallion were processed using different methods for retrieval of the epididymal spermatozoa and were pooled and cryopreserved either using 5% glycerol or 5% dimethyl formamide (DMF) as cryoprotectant. The semen quality parameters viz., progressive motility, HOST, viability and acrosome integrity were evaluated at the fresh, pre-freeze and post-thaw stages.Results: Retrograde method of flushing of epididymis yielded significantly (p < 0.05) higher concentration of the stallion sperm than that of the floating method. The qualitative semen parameters i.e., viability, plasma membrane integrity and acrosome integrity were found to be significantly restored using 5% DMF as cryoprotectant in comparison to when 5% glycerol was used.Conclusion: Retrograde flushing method of epididymis yielded significantly higher sperm concentration to that of the floating method, and 5% DMF as cryoprotectant provided acceptable freezability of stallion epididymal spermatozoa. DOI: 10.54680/fr23310110312.","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":"44 3","pages":"134-141"},"PeriodicalIF":1.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50160974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0