Cryo lettersPub Date : 2023-07-01DOI: 10.54680/fr23410110512
Y. S. Sales, Jéssica SalesLobato, Carla Tatiana Nascimento Sousa Vieira, João Eudes Faria Cavalcante Filho, Yasmim Maia Ferreira, Marcos Luiz da Silva Apoliano, R. V. Nascimento, Silvio Alencar Căndido Sobrinho, José Ariévilo Gurgel Rodrigues, Carla Pamela Braga Guia, Fernanda Vitória Almeida Magalhães, C. Salmito‐Vanderley
{"title":"Supplementation of sulfate polysaccharides in the seminal cooling medium of common curimata (Prochilodus brevis).","authors":"Y. S. Sales, Jéssica SalesLobato, Carla Tatiana Nascimento Sousa Vieira, João Eudes Faria Cavalcante Filho, Yasmim Maia Ferreira, Marcos Luiz da Silva Apoliano, R. V. Nascimento, Silvio Alencar Căndido Sobrinho, José Ariévilo Gurgel Rodrigues, Carla Pamela Braga Guia, Fernanda Vitória Almeida Magalhães, C. Salmito‐Vanderley","doi":"10.54680/fr23410110512","DOIUrl":"https://doi.org/10.54680/fr23410110512","url":null,"abstract":"BACKGROUND\u0000The use of sulfated polysaccharides (PS) in seminal cooling is known to improve seminal quality.\u0000\u0000\u0000OBJECTIVE\u0000To evaluate the effect of different concentrations of PS, extracted from the macroalgae Gracilaria domigensis as a supplement to the seminal cooling medium of the reophilic fish Prochilodus brevis (common curimatã).\u0000\u0000\u0000MATERIALS AND METHODS\u0000Five semen pools were diluted in ACP-104 (treatment T1), in BTS® (T2) and in BTS® with different concentrations of PS (0.5 [T3]; 1.0 [T4] and 1.5 [T5]). The samples were cooled for different times (0, 6, 24, 48, 72, 96 and 120 h) and after each hour they were analyzed for: morphology, membrane integrity, DNA integrity and sperm kinetics.\u0000\u0000\u0000RESULTS\u0000There were no significant differences between the treatments containing different concentrations of sulfated polysaccharides. Regarding the different cooling times, it was possible to observe that after hour 96, there was a reduction in the parameters of sperm kinetics. For DNA integrity there was no significant difference in relation to the treatments nor in relation to the hours. For membrane integrity, a reduction was noted as of hour 96, but there was no influence of polysaccharides. For the sperm morphology, there was no statistical difference between the hours, however the BTS was better than the ACP-104.\u0000\u0000\u0000CONCLUSION\u0000It is concluded that the use of polysaccharides in seminal cooling has no negative effect on sperm parameters and proves that seminal cooling keeps the material viable for up to 72 hours. Doi: 10.54680/fr23410110512.","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":"2013 1","pages":"208-218"},"PeriodicalIF":1.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86274886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cryo lettersPub Date : 2023-07-01DOI: 10.54680/fr23410110712
Kiran Parasher, Shailika Sharma, Papiya Mukherjee, P. Qazi
{"title":"Cryopreservation of zygotic embryos of Podophyllum hexandrum Royle, an endangered medicinal plant, by vitrification and v cryo-plate techniques.","authors":"Kiran Parasher, Shailika Sharma, Papiya Mukherjee, P. Qazi","doi":"10.54680/fr23410110712","DOIUrl":"https://doi.org/10.54680/fr23410110712","url":null,"abstract":"BACKGROUND\u0000Podophyllum hexandrum is a highly endangered valuable medicinal plant of the Himalayas belonging to family Berberidaceae. This plant needs conservation efforts due to the over-exploitation and unscrupulous harvesting from the wild because of its ever-increasing demand.\u0000\u0000\u0000OBJECTIVE\u0000To establish a long-term cryopreservation method for Podophyllum hexandrum using two techniques: Vitrification and V Cryo-plate.\u0000\u0000\u0000MATERIALS AND METHODS\u0000Zygotic embryos were cryopreserved using vitrification and V cryo-plate by optimization of parameters including preculture time, loading time and PVS2 dehydration time. Recovery of zygotic embryos was performed on different regrowth media for plantlet formation.\u0000\u0000\u0000RESULTS\u0000With V cryo-plate, 90% regrowth was obtained as compared to 73.3% with vitrification. V Cryo-plate conditions were pre-culture of zygotic embryos in 0.3 M sucrose for 4 days, treatment in loading solution with 0.8 M sucrose for 20 min, dehydration in PVS2 for 50 min, LN exposure, unloading in 1.2 M sucrose for 20 min and transfer of zygotic embryos to regrowth medium for recovery. During recovery, the maximum number of shoots (4.2) and highest shoot length (5.1 cm) were observed on regrowth medium with 1.5 mg per liter BAP and 0.1 mg per liter IAA (R7).\u0000\u0000\u0000CONCLUSION\u0000Zygotic embryos of Podophyllum hexandrum were cryopreserved with 90% regrowth using a V cryoplate technique and plantlets were produced directly after cryopreservation. Doi: 10.54680/fr23410110712.","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":"4 1","pages":"219-228"},"PeriodicalIF":1.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89835481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Y S Sales, J S Lobato, C T Nascimento Sousa Vieira, J E Faria Cavalcante Filho, Y M Ferreira, M L da Silva Apoliano, R V do Nascimento, S A Candido Sobrinho, J A Gurgel Rodrigues, C P Braga Guia, F V Almeida Magalhaes, C S Salmito-Vanderley
{"title":"Supplementation of sulfate polysaccharides in the seminal cooling medium of common curimata (Prochilodus brevis).","authors":"Y S Sales, J S Lobato, C T Nascimento Sousa Vieira, J E Faria Cavalcante Filho, Y M Ferreira, M L da Silva Apoliano, R V do Nascimento, S A Candido Sobrinho, J A Gurgel Rodrigues, C P Braga Guia, F V Almeida Magalhaes, C S Salmito-Vanderley","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Background: </strong>The use of sulfated polysaccharides (PS) in seminal cooling is known to improve seminal quality.</p><p><strong>Objective: </strong>To evaluate the effect of different concentrations of PS, extracted from the macroalgae Gracilaria domigensis as a supplement to the seminal cooling medium of the reophilic fish Prochilodus brevis (common curimatã).</p><p><strong>Materials and methods: </strong>Five semen pools were diluted in ACP-104 (treatment T1), in BTS® (T2) and in BTS® with different concentrations of PS (0.5 [T3]; 1.0 [T4] and 1.5 [T5]). The samples were cooled for different times (0, 6, 24, 48, 72, 96 and 120 h) and after each hour they were analyzed for: morphology, membrane integrity, DNA integrity and sperm kinetics.</p><p><strong>Results: </strong>There were no significant differences between the treatments containing different concentrations of sulfated polysaccharides. Regarding the different cooling times, it was possible to observe that after hour 96, there was a reduction in the parameters of sperm kinetics. For DNA integrity there was no significant difference in relation to the treatments nor in relation to the hours. For membrane integrity, a reduction was noted as of hour 96, but there was no influence of polysaccharides. For the sperm morphology, there was no statistical difference between the hours, however the BTS was better than the ACP-104.</p><p><strong>Conclusion: </strong>It is concluded that the use of polysaccharides in seminal cooling has no negative effect on sperm parameters and proves that seminal cooling keeps the material viable for up to 72 hours. Doi: 10.54680/fr23410110512.</p>","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":"44 4","pages":"208-218"},"PeriodicalIF":1.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50160984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cryo lettersPub Date : 2023-07-01DOI: 10.54680/fr23410110212
Khursheed Ahmad Sofi, Beenish Qureshi
{"title":"Strip pulled straw: cost effective alternative cryodevice for the vitrification of oocytes.","authors":"Khursheed Ahmad Sofi, Beenish Qureshi","doi":"10.54680/fr23410110212","DOIUrl":"https://doi.org/10.54680/fr23410110212","url":null,"abstract":"BACKGROUND\u0000Increased cooling and warming rates by using a suitable cryodevice allows the use of lower cryoprotectant concentration and reduces cryoinjuries as a result of the rapid passage through the 'dangerous' temperature zone.\u0000\u0000\u0000OBJECTIVE\u0000To evaluate the effectiveness of newly customized strip pulled straw (SPS) with respect to post warming quality, viability, and in vitro maturation for immature oocytes post-vitrification of.\u0000\u0000\u0000MATERIALS AND METHODS\u0000SPS was prepared using conventional French mini straw to combine the merits of OPS and the Cryotop system. Immature sheep oocytes were treated in 15% EG + 15% DMSO, loaded on SPS and plunged into liquid nitrogen (LN). Post thaw quality, viability, and maturation rates of oocytes were determined after 1 week storage in LN.\u0000\u0000\u0000RESULTS\u0000SPS achieved a post-thaw morphological survival of 90.9% with 9.0% morphological defects, 96.4% viability and 51% in vitro maturation. In comparison to OPS, SPS had higher post thaw survival (86.5% vs 67.9%) and maturation rate (57.7% vs 50.5%) with lower morphological defects (13.5% vs 32.1%). Cumulus cell loss was the highest among morphological abnormalities of post warm oocytes in SPS (40.9%) and OPS (44.1%). The data showed acceptable post thaw survival, viability and in vitro maturation rate of immature ovine oocytes using SPS as compared to traditional OPS.\u0000\u0000\u0000CONCLUSION\u0000SPS can be used as a cost effective alternative device for oocyte vitrification. Doi: 10.54680/fr23410110212.","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":"31 1","pages":"229-233"},"PeriodicalIF":1.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82174774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Strip pulled straw: cost effective alternative cryodevice for the vitrification of oocytes.","authors":"K A Sofi, B Qureshi","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Background: </strong>Increased cooling and warming rates by using a suitable cryodevice allows the use of lower cryoprotectant concentration and reduces cryoinjuries as a result of the rapid passage through the 'dangerous' temperature zone.</p><p><strong>Objective: </strong>To evaluate the effectiveness of newly customized strip pulled straw (SPS) with respect to post warming quality, viability, and in vitro maturation for immature oocytes post-vitrification of.</p><p><strong>Materials and methods: </strong>SPS was prepared using conventional French mini straw to combine the merits of OPS and the Cryotop system. Immature sheep oocytes were treated in 15% EG + 15% DMSO, loaded on SPS and plunged into liquid nitrogen (LN). Post thaw quality, viability, and maturation rates of oocytes were determined after 1 week storage in LN.</p><p><strong>Results: </strong>SPS achieved a post-thaw morphological survival of 90.9% with 9.0% morphological defects, 96.4% viability and 51% in vitro maturation. In comparison to OPS, SPS had higher post thaw survival (86.5% vs 67.9%) and maturation rate (57.7% vs 50.5%) with lower morphological defects (13.5% vs 32.1%). Cumulus cell loss was the highest among morphological abnormalities of post warm oocytes in SPS (40.9%) and OPS (44.1%). The data showed acceptable post thaw survival, viability and in vitro maturation rate of immature ovine oocytes using SPS as compared to traditional OPS.</p><p><strong>Conclusion: </strong>SPS can be used as a cost effective alternative device for oocyte vitrification. Doi: 10.54680/fr23410110212.</p>","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":"44 4","pages":"229-233"},"PeriodicalIF":1.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50160983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P L Fracaro, C D Corcini, N L Souza Gatti, J S Filho, I B Acosta, J S Filho, F P Hartwig, B D Rosa Curcio, A S Varela Junior
{"title":"Freezing of equine semen is influenced by exposure time and concentration of the cryoprotectant glycerol.","authors":"P L Fracaro, C D Corcini, N L Souza Gatti, J S Filho, I B Acosta, J S Filho, F P Hartwig, B D Rosa Curcio, A S Varela Junior","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Background: </strong>Glycerol is a cryoprotectant widely used in the freezing of mammalian semen, but no study has demonstrated its optimum concentration and the appropriate exposure time for equine species.</p><p><strong>Objective: </strong>To demonstrate that the exposure time (15, 30, 45, 60, 75 and 90 min) versus concentration (2, 3, 4 and 5%) of the cryoprotectant glycerol influences the freezing success of equine semen.</p><p><strong>Materials and methods: </strong>The ejaculate of 12 stallions were frozen in different glycerol concentrations following different exposure times. The thawed sperm was evaluated for kinetic parameters using a Computer Assisted Semen Analysis (CASA) system and cell feature parameters were assessed by flow cytometry.</p><p><strong>Results: </strong>Considering the total and progressive motility of the spermatozoa, we concluded that protocols using 5% glycerol for 15 and 30 min exposure, 4% glycerol for 45 min exposure and 3% glycerol for 90 min exposure generated the best results.</p><p><strong>Conclusion: </strong>We suggest the use of any of these protocols for a better cryopreservation of equine semen. Doi: 10.54680/fr23410110412.</p>","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":"44 4","pages":"234-239"},"PeriodicalIF":1.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50160981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cryo lettersPub Date : 2023-07-01DOI: 10.54680/fr23410110312
S. Diantina, Craig McGill, A. C. McCormick, J. Millner, Hugh W. Pritchard, J. Nadarajan
{"title":"Comparative seed cryopreservation of indonesian and new zealand epiphytic and terrestrial orchids.","authors":"S. Diantina, Craig McGill, A. C. McCormick, J. Millner, Hugh W. Pritchard, J. Nadarajan","doi":"10.54680/fr23410110312","DOIUrl":"https://doi.org/10.54680/fr23410110312","url":null,"abstract":"BACKGROUND\u0000The atypical seed storage behaviour reported in several orchid species justifies cryopreservation as a complementary conservation strategy to conventional seed banking.\u0000\u0000\u0000OBJECTIVE\u0000This study aimed to assess the seed cryopreservation potential of five orchid species; two tropical epiphytic, Indonesian species (Dendrobium strebloceras, D. lineale), one temperate epiphytic, New Zealand species (D. cunninghamii) and two temperate terrestrial, New Zealand species (Pterostylis banksii, Thelymitra nervosa).\u0000\u0000\u0000MATERIALS AND METHODS\u0000Seeds were cryopreserved by direct immersion in liquid nitrogen (LN) and through the application of a cryoprotectant vitrification method. For the latter, seeds were exposed to Plant Vitrification Solution 2 (PVS2) for 0, 20, 50, and 70 min, at either room temperature or on ice, prior to immersion in LN.\u0000\u0000\u0000RESULTS\u0000Seeds of all the studied species germinated well following direct cooling in LN. There was no difference in the seedling development capability between cryopreserved and non-cryopreserved seeds of both tropical epiphytic species and direct immersion in LN enhanced seed germination and shoot formation in both temperate terrestrials.\u0000\u0000\u0000CONCLUSION\u0000Through a range of analyses of germination and post-germination growth, our study shows the potential for cryopreserving epiphytic or terrestrial orchids from tropical and temperate regions. Doi: 10.54680/fr23410110312.","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":"73 1","pages":"197-207"},"PeriodicalIF":1.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84321569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Cryopreservation of zygotic embryos of Podophyllum hexandrum Royle, an endangered medicinal plant, by vitrification and v cryo-plate techniques.","authors":"K Parasher, S Sharma, P Mukherjee, P H Qazi","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Background: </strong>Podophyllum hexandrum is a highly endangered valuable medicinal plant of the Himalayas belonging to family Berberidaceae. This plant needs conservation efforts due to the over-exploitation and unscrupulous harvesting from the wild because of its ever-increasing demand.</p><p><strong>Objective: </strong>To establish a long-term cryopreservation method for Podophyllum hexandrum using two techniques: Vitrification and V Cryo-plate.</p><p><strong>Materials and methods: </strong>Zygotic embryos were cryopreserved using vitrification and V cryo-plate by optimization of parameters including preculture time, loading time and PVS2 dehydration time. Recovery of zygotic embryos was performed on different regrowth media for plantlet formation.</p><p><strong>Results: </strong>With V cryo-plate, 90% regrowth was obtained as compared to 73.3% with vitrification. V Cryo-plate conditions were pre-culture of zygotic embryos in 0.3 M sucrose for 4 days, treatment in loading solution with 0.8 M sucrose for 20 min, dehydration in PVS2 for 50 min, LN exposure, unloading in 1.2 M sucrose for 20 min and transfer of zygotic embryos to regrowth medium for recovery. During recovery, the maximum number of shoots (4.2) and highest shoot length (5.1 cm) were observed on regrowth medium with 1.5 mg per liter BAP and 0.1 mg per liter IAA (R7).</p><p><strong>Conclusion: </strong>Zygotic embryos of Podophyllum hexandrum were cryopreserved with 90% regrowth using a V cryoplate technique and plantlets were produced directly after cryopreservation. Doi: 10.54680/fr23410110712.</p>","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":"44 4","pages":"219-228"},"PeriodicalIF":1.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50160980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cryo lettersPub Date : 2023-07-01DOI: 10.54680/fr23410110412
Pablo Luis Fracaro, C. Corcini, Norton Luis Souza Gatti, Jorge Squeff Filho, I. B. Acosta, Felipe Pires Hartwig, Bruna Da Rosa Curcio, A. S. V. Junior
{"title":"Freezing of equine semen is influenced by exposure time and concentration of the cryoprotectant glycerol.","authors":"Pablo Luis Fracaro, C. Corcini, Norton Luis Souza Gatti, Jorge Squeff Filho, I. B. Acosta, Felipe Pires Hartwig, Bruna Da Rosa Curcio, A. S. V. Junior","doi":"10.54680/fr23410110412","DOIUrl":"https://doi.org/10.54680/fr23410110412","url":null,"abstract":"BACKGROUND\u0000Glycerol is a cryoprotectant widely used in the freezing of mammalian semen, but no study has demonstrated its optimum concentration and the appropriate exposure time for equine species.\u0000\u0000\u0000OBJECTIVE\u0000To demonstrate that the exposure time (15, 30, 45, 60, 75 and 90 min) versus concentration (2, 3, 4 and 5%) of the cryoprotectant glycerol influences the freezing success of equine semen.\u0000\u0000\u0000MATERIALS AND METHODS\u0000The ejaculate of 12 stallions were frozen in different glycerol concentrations following different exposure times. The thawed sperm was evaluated for kinetic parameters using a Computer Assisted Semen Analysis (CASA) system and cell feature parameters were assessed by flow cytometry.\u0000\u0000\u0000RESULTS\u0000Considering the total and progressive motility of the spermatozoa, we concluded that protocols using 5% glycerol for 15 and 30 min exposure, 4% glycerol for 45 min exposure and 3% glycerol for 90 min exposure generated the best results.\u0000\u0000\u0000CONCLUSION\u0000We suggest the use of any of these protocols for a better cryopreservation of equine semen. Doi: 10.54680/fr23410110412.","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":"12 1","pages":"234-239"},"PeriodicalIF":1.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87084319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S Diantina, C McGill, A C McCormick, J Millner, H W Pritchard, J Nadarajan
{"title":"Comparative seed cryopreservation of indonesian and new zealand epiphytic and terrestrial orchids.","authors":"S Diantina, C McGill, A C McCormick, J Millner, H W Pritchard, J Nadarajan","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Background: </strong>The atypical seed storage behaviour reported in several orchid species justifies cryopreservation as a complementary conservation strategy to conventional seed banking.</p><p><strong>Objective: </strong>This study aimed to assess the seed cryopreservation potential of five orchid species; two tropical epiphytic, Indonesian species (Dendrobium strebloceras, D. lineale), one temperate epiphytic, New Zealand species (D. cunninghamii) and two temperate terrestrial, New Zealand species (Pterostylis banksii, Thelymitra nervosa).</p><p><strong>Materials and methods: </strong>Seeds were cryopreserved by direct immersion in liquid nitrogen (LN) and through the application of a cryoprotectant vitrification method. For the latter, seeds were exposed to Plant Vitrification Solution 2 (PVS2) for 0, 20, 50, and 70 min, at either room temperature or on ice, prior to immersion in LN.</p><p><strong>Results: </strong>Seeds of all the studied species germinated well following direct cooling in LN. There was no difference in the seedling development capability between cryopreserved and non-cryopreserved seeds of both tropical epiphytic species and direct immersion in LN enhanced seed germination and shoot formation in both temperate terrestrials.</p><p><strong>Conclusion: </strong>Through a range of analyses of germination and post-germination growth, our study shows the potential for cryopreserving epiphytic or terrestrial orchids from tropical and temperate regions. Doi: 10.54680/fr23410110312.</p>","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":"44 4","pages":"197-207"},"PeriodicalIF":1.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50160978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}