Cryo letters最新文献_第9页

Assessing the benefits of antioxidant and antibiotics treatments for cryopreservation of the model alga Ectocarpus siliculosus and the endemic alga Acinetospora asiatica. 抗氧化和抗生素处理对模式藻硅酸外露和特有藻亚洲动孢的低温保存效果评估。

IF 1 4区生物学

Cryo letters Pub Date : 2023-05-01 DOI: 10.54680/fr23310110212

José Avila-Peltroche, Boo Yeon Won, Tae Oh Cho

{"title":"Assessing the benefits of antioxidant and antibiotics treatments for cryopreservation of the model alga Ectocarpus siliculosus and the endemic alga Acinetospora asiatica.","authors":"José Avila-Peltroche, Boo Yeon Won, Tae Oh Cho","doi":"10.54680/fr23310110212","DOIUrl":"https://doi.org/10.54680/fr23310110212","url":null,"abstract":"BACKGROUND\u0000Cryopreservation in liquid nitrogen is a suitable technique for preserving seaweeds, a group of photosynthetic organisms with many applications. Although there are some standard protocols for seaweed cryopreservation, most rely on expensive controlled-rate coolers. Moreover, several factors, such as the use of antioxidants or antibiotics, remain unexplored.\u0000\u0000\u0000OBJECTIVE\u0000To test the effect of 2-mercapthoethanol (antioxidant) and antibiotic mixtures on the cryopreservation of the model alga Ectocarpus siliculosus and the endemic brown seaweed Acinetospora asiatica using a low-tech passive rate cooler.\u0000\u0000\u0000MATERIALS AND METHODS\u00002-mercaptoethanol was added to the cryoprotectant (CPA) solution, while antibiotic mixtures were included in the culture medium during the recovery process. In addition, two CPA solutions were tested on E. siliculosus.\u0000\u0000\u0000RESULTS\u0000After two weeks of recovery, the treatment comprising PSC antibiotic mixture (Penicillin G, streptomycin, and chloramphenicol) showed a significant increase in post-thaw viability. Antioxidant treatment did not improve viability. The highest viabilities for E. siliculosus and A. asiatica were 64-83%, and 83-87%, respectively, using 10% glycerol + 10% proline as CPA solution.\u0000\u0000\u0000CONCLUSION\u0000E. siliculosus and A. asiatica were successfully cryopreserved using a low-tech passive rate cooler, 10% glycerol + 10% proline solution, and antibiotic treatment. The highest post-thaw viabilities (64-87%) reported for PSC antibiotic mixture suggest the potential benefits of using antibiotics during post-thaw recovery of marine macroalgae. This study is the first report on the cryopreservation of A. asiatica. DOI: 10.54680/fr23310110212.","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82522047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The safety of human embryos following long-term cryopreservation ( >6 years) on vitrification. 玻璃化长期冷冻保存(>6年)后人类胚胎的安全性。

IF 1 4区生物学

Cryo letters Pub Date : 2023-05-01 DOI: 10.54680/fr23310110612

H. He, Rui Jiang, X. Ren, L. Jin, Yaping Jiang

{"title":"The safety of human embryos following long-term cryopreservation ( >6 years) on vitrification.","authors":"H. He, Rui Jiang, X. Ren, L. Jin, Yaping Jiang","doi":"10.54680/fr23310110612","DOIUrl":"https://doi.org/10.54680/fr23310110612","url":null,"abstract":"BACKGROUND\u0000Vitrification of embryos has become the basic means of assisted reproductive technology (ART) therapy in recent years. Concerns have also been raised about the safety of vitrification and the effect of cryopreservation time. Most of the previous studies were on the data within 6 years of cryopreservation.\u0000\u0000\u0000OBJECTIVE\u0000In this study, we aimed to evaluate the impact of long-term cryopreservation (>6 years) on pregnancy and neonatal outcomes.\u0000\u0000\u0000MATERIALS AND METHODS\u0000This research was a single-center, retrospective analysis, including 426 frozen-thawed embryo transfer (FET) cycles. Patients who participated in IVF-FET cycles between January 2013 to December 2020 were analyzed. Preferentially matched participants were divided into three groups according to storage time: group A (>72 months), group B (0-3 months, propensity score matching [PSM] according to the age of oocyte retrieval), and group C (0-3 months, PSM according to the age of embryo transfer).\u0000\u0000\u0000RESULTS\u0000Our results revealed that there were no significant differences in human chorionic gonadotropin [HCG] positive rate, clinical pregnancy rate, miscarriage rate, live birth rate, and neonatal outcomes when the embryo storage duration >72 months. But the proportion of high birth weight was higher in group A (>72 months) when matched according to age at embryo transfer.\u0000\u0000\u0000CONCLUSION\u0000The results of our study showed that long-term cryopreservation had no effect on the pregnancy and neonatal outcomes of vitrification. The results offer evidence for the safety of using long-term cryopreservation embryos after vitrification. DOI: 10.54680/fr23310110612.","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76679147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Cryoprotectant and Pre-Freezing on the Sperm Motility, Viability and Fertility of Goldfish Carassius Auratus (Pisces: Cyprinidae) Post Short-Term Cryopreservation 冷冻保护剂和预冷冻对鲫鱼(Carassius Auratus)短期冷冻后精子活力、活力和生育能力的影响

IF 1 4区生物学

Cryo letters Pub Date : 2023-05-01 DOI: 10.54680/fr23310110412

N. Nurlaili, K. Eriani, I. Salma, S. Maulida, Sri Riska Rahayu, Luvi Syafrida Handayani, F. Kocabaş, M. N. Siti-Azizah, M. Wilkes, Z. Muchlisin

{"title":"Effect of Cryoprotectant and Pre-Freezing on the Sperm Motility, Viability and Fertility of Goldfish Carassius Auratus (Pisces: Cyprinidae) Post Short-Term Cryopreservation","authors":"N. Nurlaili, K. Eriani, I. Salma, S. Maulida, Sri Riska Rahayu, Luvi Syafrida Handayani, F. Kocabaş, M. N. Siti-Azizah, M. Wilkes, Z. Muchlisin","doi":"10.54680/fr23310110412","DOIUrl":"https://doi.org/10.54680/fr23310110412","url":null,"abstract":"BACKGRUND: Goldfish Carassius auratus is a popular ornamental fish extensively cultured worldwide. Sperm cryopreservation is a common fish breeding method that ensures sperm availability around the year. Studies on cryopreservation of goldfish sperm, especially on the\u0000 suitability of cryoprotectant types and pre-freezing time, are scarcely available. OBJECTIVE: To determine the most suitable type of cryoprotectant and pre-freezing for the successful cryopreservation of goldfish sperm. MATERIALS AND METHODS: A completely randomized design with\u0000 two factors was utilized in this study. The first factor is the type of cryoprotectants, which included methanol, ethanol, ethylene glycol, glycerol, and DMSO. The second is pre-freezing times of 10, 20, 30, and 40 min at each of the pre- freezing temperatures of 4°C, −10°C,\u0000 and −79°C, meaning that the total times for the ramping down of temperature were 30, 60, 90 and 120 min, respectively. The Ringer solution and 10% egg yolk were used as extender and extracellular cryoprotectant. The sperm was stored at −179°C for 7 days. RESULTS:\u0000 The ANOVA test showed that cryoprotectants and pre-freezing significantly affected the motility, viability, and fertility of goldfish sperm after freezing in liquid nitrogen for 7 days (ρ<0.05). Furthermore, 10% DMSO combined with 15% egg yolk with an pre-freezing time\u0000 of 20 min can maintain sperm motility, viability, and fertility higher than other treatments, by 79%, 80%, and 33%, respectively. The agarose gel electrophoresis showed no DNA fragmentation in all samples, including fresh sperm. CONCLUSION: We conclude that 10%\u0000 DMSO combined with 15% egg yolk and 20 min pre-freezing is the best treatment for goldfish sperm cryopreservation.","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74828390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of isolation protocols and cryoprotectants on freezing of stallion epididymal spermatozoa. 分离方案和冷冻保护剂对种马附睾精子冷冻的影响。

IF 1 4区生物学

Cryo letters Pub Date : 2023-05-01

T R Talluri, D Jhamb, N Paul, J Singh, R K Dedar, S C Mehta, R A Legha, Y Pal

{"title":"Effect of isolation protocols and cryoprotectants on freezing of stallion epididymal spermatozoa.","authors":"T R Talluri, D Jhamb, N Paul, J Singh, R K Dedar, S C Mehta, R A Legha, Y Pal","doi":"","DOIUrl":"","url":null,"abstract":"Background: The recovery of spermatozoa from the cauda epididymis may be the only option to obtain genetic material from elite stallions that had undergone castration or sudden death due to colic or severe injury.Objective: To evaluate two different protocols for retrieval of stallion epididymal spermatozoa and to evaluate different cryoprotectants on the freezability of the epididymal spermatozoa.Materials and methods: Six epididymides from three stallions were collected immediately after routine castration under general anesthesia. In the first experiment, each epididymis (of two testes) of the same stallion were processed using different methods for retrieval of the epididymal spermatozoa and were pooled and cryopreserved either using 5% glycerol or 5% dimethyl formamide (DMF) as cryoprotectant. The semen quality parameters viz., progressive motility, HOST, viability and acrosome integrity were evaluated at the fresh, pre-freeze and post-thaw stages.Results: Retrograde method of flushing of epididymis yielded significantly (p < 0.05) higher concentration of the stallion sperm than that of the floating method. The qualitative semen parameters i.e., viability, plasma membrane integrity and acrosome integrity were found to be significantly restored using 5% DMF as cryoprotectant in comparison to when 5% glycerol was used.Conclusion: Retrograde flushing method of epididymis yielded significantly higher sperm concentration to that of the floating method, and 5% DMF as cryoprotectant provided acceptable freezability of stallion epididymal spermatozoa. DOI: 10.54680/fr23310110312.","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50160974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of cryopreservation techniques in manufacturing, transport, and storage of Car-T therapy products. 冷冻保存技术在Car-T治疗产品的制造、运输和储存中的作用。

IF 1 4区生物学

Cryo letters Pub Date : 2023-05-01

M Jandova, G N Stacey, M Lanska, I Gregor, P Rozsivalova, L Bekova, Z W Duchacova, D Belada, J Radocha, P Mericka, B Fuller

{"title":"The role of cryopreservation techniques in manufacturing, transport, and storage of Car-T therapy products.","authors":"M Jandova, G N Stacey, M Lanska, I Gregor, P Rozsivalova, L Bekova, Z W Duchacova, D Belada, J Radocha, P Mericka, B Fuller","doi":"","DOIUrl":"","url":null,"abstract":"Several clinical trials have proved the efficacy and safety of T-cells chimeric antigen receptor (CAR-T cells) in treatment of malignant lymphoma and the first products were registered in the European Union in 2018. The shelf-life of CAR-T cell products in the liquid state is short, so cryopreservation offers a significant benefit for logistics in manufacturing and patient management. Direct shipment of the cryopreserved CAR-T cell therapy products to the clinical department is feasible, nevertheless, intermediate storage in the hospital cryostorage facility gives significant advantage in planning of their administration to patients. Moreover, some manufacturers prefer transport of the starting material cryopreserved at the collection site. The cryopreservation protocol used for starting material by the authors is based on combining dimethyl sulphoxide (DMSO) with hydroxyethyl starch (HES) and slow controlled cooling in cryobags housed in metal cassettes. This achieves the mononuclear cell post-thaw viability of 98.8 ± 0.5 % and recovery of 72.8, ± 10.2 %. Transport of the starting material to the manufactures and return transport of the CAR-T therapy product is performed by authorized courier companies. Intermediate cryostorage of the final CAR-T cell therapy product is performed in a separate dry-storage liquid nitrogen container. On the day of infusion, the cryopreserved products are transported to the clinical department in a dry shipper. On the wards the product is removed from the cassette, inserted into a sterile plastic bag, thawed in a 37 degree C water bath followed by immediate intravenous administration. The authors discuss the adherence of the used technology to good manufacturing practice (GMP) principles and genetic safety assurance rules. Doi: 10.54680/fr23310110112.","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50160976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aqueous extract OF Moringa oleifera Lam leaves added to freezing extenders damages goat sperm membranes. 辣木叶的水提取物添加到冷冻填充剂中会破坏山羊精子膜。

IF 1 4区生物学

Cryo letters Pub Date : 2023-05-01

D C de Mello Seal, M Monteiro, L C Pereira Arruda, J H de Souza, R R de Vasconcelos Alves, L A Lira Soares, T H Napoleao, P M Guedes Paiva, P E Bezerra de Lima, R C Bressan Queiroz de Figueiredo, M M Pessoa Guerra

{"title":"Aqueous extract OF Moringa oleifera Lam leaves added to freezing extenders damages goat sperm membranes.","authors":"D C de Mello Seal, M Monteiro, L C Pereira Arruda, J H de Souza, R R de Vasconcelos Alves, L A Lira Soares, T H Napoleao, P M Guedes Paiva, P E Bezerra de Lima, R C Bressan Queiroz de Figueiredo, M M Pessoa Guerra","doi":"","DOIUrl":"","url":null,"abstract":"Background: Semen cryopreservation is a biotechnology used frequently in animal production; however, there are some obstacles, such as those caused by high levels of reactive oxygen species (ROS). Moringa oleifera (MO) is known as a potent source of antioxidants and might be an important adjuvant.Objective: The objective of this study was to determine the effect of different concentrations of MO extract supplementation on goat semen cryopreservation efficiency.Materials and methods: Ejaculates (n=6) from four goat breeders were pooled and diluted in skimmed milk (SM) or Tris-egg yolk (TEY)-based extenders and supplemented with different concentrations of MO extract (0, 1, 2 and 5 mg/mL). After the freeze-thaw cycle, sperm kinetics and viability were assessed.Results: With the SM extender, straightness, wobble and plasma membrane integrity were lower than in the control group (P < 0.05). With the TEY extender, wobble was lower in with 5 mg/mL MO extract than in the control group (P < 0.05). As regards sperm ultrastructure, evaluated by SEM, the MO extract, regardless of the diluent used, damaged the membrane of sperm cells in a dose-dependent manner.Conclusion: The addition of aqueous extract of MO leaves in both diluents at all concentrations tested affects the parameters of sperm progressivity and damages the plasma membrane in a dose-dependent manner. DOI: 10.54680/fr23310110712.","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50161060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of isolation protocols and cryoprotectants on freezing of stallion epididymal spermatozoa. 分离方案和冷冻保护剂对种马附睾精子冷冻的影响。

IF 1 4区生物学

Cryo letters Pub Date : 2023-05-01 DOI: 10.54680/fr23310110312

T. Talluri, D. Jhamb, N. Paul, J. Singh, R. Dedar, S. C. Mehta, R. Legha, Y. Pal

{"title":"Effect of isolation protocols and cryoprotectants on freezing of stallion epididymal spermatozoa.","authors":"T. Talluri, D. Jhamb, N. Paul, J. Singh, R. Dedar, S. C. Mehta, R. Legha, Y. Pal","doi":"10.54680/fr23310110312","DOIUrl":"https://doi.org/10.54680/fr23310110312","url":null,"abstract":"BACKGROUND\u0000The recovery of spermatozoa from the cauda epididymis may be the only option to obtain genetic material from elite stallions that had undergone castration or sudden death due to colic or severe injury.\u0000\u0000\u0000OBJECTIVE\u0000To evaluate two different protocols for retrieval of stallion epididymal spermatozoa and to evaluate different cryoprotectants on the freezability of the epididymal spermatozoa.\u0000\u0000\u0000MATERIALS AND METHODS\u0000Six epididymides from three stallions were collected immediately after routine castration under general anesthesia. In the first experiment, each epididymis (of two testes) of the same stallion were processed using different methods for retrieval of the epididymal spermatozoa and were pooled and cryopreserved either using 5% glycerol or 5% dimethyl formamide (DMF) as cryoprotectant. The semen quality parameters viz., progressive motility, HOST, viability and acrosome integrity were evaluated at the fresh, pre-freeze and post-thaw stages.\u0000\u0000\u0000RESULTS\u0000Retrograde method of flushing of epididymis yielded significantly (p < 0.05) higher concentration of the stallion sperm than that of the floating method. The qualitative semen parameters i.e., viability, plasma membrane integrity and acrosome integrity were found to be significantly restored using 5% DMF as cryoprotectant in comparison to when 5% glycerol was used.\u0000\u0000\u0000CONCLUSION\u0000Retrograde flushing method of epididymis yielded significantly higher sperm concentration to that of the floating method, and 5% DMF as cryoprotectant provided acceptable freezability of stallion epididymal spermatozoa. DOI: 10.54680/fr23310110312.","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89585374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessing the benefits of antioxidant and antibiotics treatments for cryopreservation of the model alga Ectocarpus siliculosus and the endemic alga Acinetospora asiatica. 评估抗氧化剂和抗生素处理对模式藻类Ectocarpus siliculosus和地方藻类亚洲酸藻冷冻保存的益处。

IF 1 4区生物学

Cryo letters Pub Date : 2023-05-01

J Avila-Peltroche, B Y Won, T O Cho

{"title":"Assessing the benefits of antioxidant and antibiotics treatments for cryopreservation of the model alga Ectocarpus siliculosus and the endemic alga Acinetospora asiatica.","authors":"J Avila-Peltroche, B Y Won, T O Cho","doi":"","DOIUrl":"","url":null,"abstract":"Background: Cryopreservation in liquid nitrogen is a suitable technique for preserving seaweeds, a group of photosynthetic organisms with many applications. Although there are some standard protocols for seaweed cryopreservation, most rely on expensive controlled-rate coolers. Moreover, several factors, such as the use of antioxidants or antibiotics, remain unexplored.Objective: To test the effect of 2-mercapthoethanol (antioxidant) and antibiotic mixtures on the cryopreservation of the model alga Ectocarpus siliculosus and the endemic brown seaweed Acinetospora asiatica using a low-tech passive rate cooler.Materials and methods: 2-mercaptoethanol was added to the cryoprotectant (CPA) solution, while antibiotic mixtures were included in the culture medium during the recovery process. In addition, two CPA solutions were tested on E. siliculosus.Results: After two weeks of recovery, the treatment comprising PSC antibiotic mixture (Penicillin G, streptomycin, and chloramphenicol) showed a significant increase in post-thaw viability. Antioxidant treatment did not improve viability. The highest viabilities for E. siliculosus and A. asiatica were 64-83%, and 83-87%, respectively, using 10% glycerol + 10% proline as CPA solution.Conclusion: E. siliculosus and A. asiatica were successfully cryopreserved using a low-tech passive rate cooler, 10% glycerol + 10% proline solution, and antibiotic treatment. The highest post-thaw viabilities (64-87%) reported for PSC antibiotic mixture suggest the potential benefits of using antibiotics during post-thaw recovery of marine macroalgae. This study is the first report on the cryopreservation of A. asiatica. DOI: 10.54680/fr23310110212.","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50161061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Perspective: The Role of Cryopreservation Techniques in Manufacturing, Transport, and Storage of Car-T Therapy Products 展望:低温保存技术在Car-T治疗产品的制造、运输和储存中的作用

IF 1 4区生物学

Cryo letters Pub Date : 2023-05-01 DOI: 10.54680/fr23310110112

Miroslava Jandová, G. Stacey, M. Lánská, Jií Gregor, P. Rozsívalová, L. Beková, Zuzana Woidigová Ducháová, D. Belada, J. Radocha, P. Měřička, B. Fuller

{"title":"Perspective: The Role of Cryopreservation Techniques in Manufacturing, Transport, and Storage of Car-T Therapy Products","authors":"Miroslava Jandová, G. Stacey, M. Lánská, Jií Gregor, P. Rozsívalová, L. Beková, Zuzana Woidigová Ducháová, D. Belada, J. Radocha, P. Měřička, B. Fuller","doi":"10.54680/fr23310110112","DOIUrl":"https://doi.org/10.54680/fr23310110112","url":null,"abstract":"Several clinical trials have proved the efficacy and safety of T-cells chimeric antigen receptor (CAR-T cells) in treatment of malignant lymphoma and the first products were registered in the European Union in 2018. The shelf-life of CAR-T cell products in the liquid state is short,\u0000 so cryopreservation offers a significant benefit for logistics in manufacturing and patient management. Direct shipment of the cryopreserved CAR-T cell therapy products to the clinical department is feasible, nevertheless, intermediate storage in the hospital cryostorage facility gives significant\u0000 advantage in planning of their administration to patients. Moreover, some manufacturers prefer transport of the starting material cryopreserved at the collection site. The cryopreservation protocol used for starting material by the authors is based on combining dimethyl sulphoxide (DMSO) with\u0000 hydroxyethyl starch (HES) and slow controlled cooling in cryobags housed in metal cassettes. This achieves the mononuclear cell post-thaw viability of 98.8 ± 0.5 % and recovery of 72.8, ± 10.2 %. Transport of the starting material to the manufactures and return\u0000 transport of the CAR-T therapy product is performed by authorized courier companies. Intermediate cryostorage of the final CAR-T cell therapy product is performed in a separate dry-storage liquid nitrogen container. On the day of infusion, the cryopreserved products are transported to the\u0000 clinical department in a dry shipper. On the wards the product is removed from the cassette, inserted into a sterile plastic bag, thawed in a 37 °C water bath followed by immediate intravenous administration. The authors discuss the adherence of the used technology to good manufacturing\u0000 practice (GMP) principles and genetic safety assurance rules.","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79967892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cryopreservation of organoids. 类器官的冷冻保存。

IF 1 4区生物学

Cryo letters Pub Date : 2023-03-01

O Rogulska, J Havelkova, Y Petrenko

{"title":"Cryopreservation of organoids.","authors":"O Rogulska, J Havelkova, Y Petrenko","doi":"","DOIUrl":"","url":null,"abstract":"Organoids represent indispensable opportunities for biomedicine, including drug discovery, cancer biology, regenerative and personalised medicine or tissue and organ transplantation. However, the lack of optimized preservation strategies limits the wide use of organoids in research or clinical fields. In this review, we present a short outline of the recent developments in organoid research and current cryopreservation strategies for organoid systems. While both vitrification and slow controlled freezing have been utilized for the cryopreservation of organoid structures or their precursor components, the controlled-rate slow freezing under protection of Me2SO remains the most common approach. The application of appropriate pre- or post-treatment strategies, like the addition of Rho-kinase or myosin inhibitors into cell culture or cryopreservation medium, can increase the recovery of complex organoid constructs post-thaw. However, the high complexity of the organoid structure and heterogeneity of cellular composition bring challenges associated with cryoprotectant distribution, distinct response of cells to the solution and freezing-induced injuries. The deficit of adequate quality control methods, which may ensure the assessment of organoid recovery in due term without prolonged re-cultivation process, represents another challenge limiting the reproducibility of current cryobanking technology. In this review, we attempt to assess the current demands and achievements in organoid cryopreservation and highlight the key questions to focus on during the development of organoid preservation technologies. Doi: 10.54680/fr23210110112.","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50161054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0